LOCUS       NZ_CP020846            12540 bp    DNA     circular CON 25-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KPN1482 plasmid pKPN1482-4, complete
            sequence.
ACCESSION   NZ_CP020846
VERSION     NZ_CP020846.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06438459
            Assembly: GCF_002156745.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12540)
  AUTHORS   Long,S.W., Olsen,R.J., Eagar,T.N., Beres,S.B., Zhao,P., Davis,J.,
            Brettin,T., Xia,F. and Musser,J.M.
  TITLE     Population Genomic Analysis of 1,777 Extended-Spectrum
            Beta-Lactamase Producing Klebsiella pneumoniae, Houston, Texas:
            Unexpected Abundance of Clonal Group 307
  JOURNAL   MBio (2017) In press
REFERENCE   2  (bases 1 to 12540)
  AUTHORS   Long,S.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2017) Pathology & Genomic Medicine, Houston
            Methodist Hospital, 6565 Fannin St, Houston, TX 77030, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 201x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2023 02:43:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,021
            CDSs (total)                      :: 5,894
            Genes (coding)                    :: 5,710
            CDSs (with protein)               :: 5,710
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 89 of 184
            Pseudo Genes (incomplete)         :: 95 of 184
            Pseudo Genes (internal stop)      :: 19 of 184
            Pseudo Genes (multiple problems)  :: 19 of 184
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020846.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12540
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN1482"
                     /isolation_source="respiratory"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPN1482-4"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7107 N 95.4006 W"
                     /collection_date="2014-06-11"
                     /collected_by="Houston Methodist Hospital"
                     /genotype="ST37; Capsule locus KL105"
     gene            6..589
                     /locus_tag="B8O09_RS30685"
                     /old_locus_tag="B8O09_30640"
                     /pseudo=""
     CDS             6..589
                     /locus_tag="B8O09_RS30685"
                     /old_locus_tag="B8O09_30640"
                     /inference="COORDINATES: protein motif:HMM:NF013704.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /name="site-specific DNA-methyltransferase"
     gene            complement(640..1317)
                     /locus_tag="B8O09_RS30690"
                     /old_locus_tag="B8O09_30645"
     CDS             complement(640..1317)
                     /locus_tag="B8O09_RS30690"
                     /old_locus_tag="B8O09_30645"
                     /inference="COORDINATES: protein motif:HMM:NF036585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_086893477.1"
                     /translation="MSREHELLKLWRQLNETQQDNFLKWMKAYEIEKIYVNHNKGYFNQ
                     KFIDNFGDRLITHYFNSNRPLTKTLFEHAFNDSLNESGMQSQLAESRTNPGYDITIQNI
                     KASLKTEAARNISQKNIHVSKWMELGKGKWVLEELLARFLAHLNNYDKIFTLRYIKPTY
                     LTFKYQLIEIPKTLLLESKNAHLVVMENSTQDPKPGYGYVFDQNGEKKFSLYFDGAQRE
                     NFK"
                     /name="hypothetical protein"
     gene            complement(1310..1552)
                     /locus_tag="B8O09_RS30695"
                     /old_locus_tag="B8O09_30650"
     CDS             complement(1310..1552)
                     /locus_tag="B8O09_RS30695"
                     /old_locus_tag="B8O09_30650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012575772.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_086893475.1"
                     /translation="MQPFDDDFSNIKTVLSENIKRYRNKNNLSQEDLAELSGLHRTYIG
                     SVERKERNVTLSTLIFIASALKTSVPDLLRKKEDE"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(2634..5621)
                     /locus_tag="B8O09_RS30710"
                     /old_locus_tag="B8O09_30660"
     CDS             complement(2634..5621)
                     /locus_tag="B8O09_RS30710"
                     /old_locus_tag="B8O09_30660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241615.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_032723026.1"
                     /translation="MARRQILSLSERESLLALPDDELTLTRMAYFSEHDLALISAHRKP
                     ASRFGFAVLLCYLKNVGFAPDKKIPPSDALLKHIASRLKLTGDLWPAYLSGRETTRREH
                     LTELYRYLGVKAFTGKIQQDCITHLLPMATRTDKGILLAEELLVWLRKNNVIIPAIDVV
                     ERTCAEAMAGGDKIVFQTLNAPLTPAHRDALDRLLESSDNQPSRLTWLLQPPGKINGKN
                     VLQHLDRLSSIESLALPEGIDRTIHQNRLLKLAREGRKMSSRDLTRFSAARRHAILVCV
                     LEEARATLTDEVIELHERMLNSLFSKAKRTQAERLQQTGKLIQSKLRQYIDVGQALSEA
                     RDSGGDPWLAIENILPWPEFVASLEETRHLARKNNFDPLHIITEKYSTLRKYAPRMLSA
                     LQLIATPAAQPLADALVVIKDMYRKQSRKVPATAPLEFVPESWRKVVITPAGIDRQYYE
                     FCALSELKGALRSGDIWVKGSRRYKNFDDYLIPEKDFDKLTPALPLPVSADYHEYITSR
                     KTLLQSRLEEVNAMAALGELPDVEISDRGVKVSPLDNCVPVQVSPLAELVYSMLPRPKI
                     TEILDEVNSWTAFTRHFSHIKNDITRPDTRLLLTTILADGINLGLTKMAEACPGSTKSS
                     LEDIQAWYIRDETYSAALAELVNAQGKRPLAAFWGDGTTSSSDGQNFRTGSSGRYAGQV
                     NPKYGQEPGRQFYTHISDQYSPFYTCIISRVRDSTHVLDGLLYHESDLEIREHYTDTAG
                     FTDHVFALMHLLGFAFCPRIRDLHDKKLFIKGKADQYPALQSLISTTSLNLKEIEIHWR
                     EVLRLATSIKQGTVTASLMLKKLASYPKQNGLAKALREIGRIERTLFMLDWFRDPALRR
                     RVQAGLNKGEARNALARAVFLHRLGEIRDRKPENQSYRASGLTLLTAAISLWNTVYMER
                     AVDALKRKGVKINEQLLSHLSPLGWEHINLTGDYIWKSNRIPASGKFRRLRPAKVERSK
                     NSLNVQ"
                     /name="Tn3 family transposase"
     gene            5788..6423
                     /locus_tag="B8O09_RS30715"
                     /old_locus_tag="B8O09_30665"
     CDS             5788..6423
                     /locus_tag="B8O09_RS30715"
                     /old_locus_tag="B8O09_30665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470624.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000470624.1"
                     /translation="MFIRAYLRASTEDQFADRAKEMLEQFVQERGHKIASYYRENISGT
                     KLDRPELGRLLMDSHRNDILLVEQIDRLTRLSNSDWMTLKKQIEQHELRIVSLDVPTSW
                     QALSDKDPSQADPITRAVITAINNMLIDLMASMSHKDWLSRRHRQKQGIERAHTLGKYR
                     GKQADQERHKKVLYYRQVKKLSIRETAEATGYSTSQVCRIQALFRPEN"
                     /name="recombinase family protein"
     gene            complement(6451..7287)
                     /gene="fghA"
                     /locus_tag="B8O09_RS30720"
                     /old_locus_tag="B8O09_30670"
     CDS             complement(6451..7287)
                     /gene="fghA"
                     /locus_tag="B8O09_RS30720"
                     /old_locus_tag="B8O09_30670"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008813493.1"
                     /GO_function="GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="WP_009652884.1"
                     /translation="MELIEQHASAGGWQNVYRHNSQTLNCGMNFGVYLPPKAATEKLPV
                     LYWLSGLTCTEQNFITKSGMQHYAARNNIIVVVPDTSPRGEHVADADSYDLGQGAGFYL
                     NATEHPWNTHYRMYDYILHELPDVVMEHLPVTSRKSISGHSMGGLGALVLSLRNPDEYV
                     SVSAFSPIVSPSQVPWGQQAFTAYLGENKKTWEHYDPVSLILQGAKLPEIFIDQGLSDA
                     FYEDQLRTKSLERVCNEMNINTSFRYHTGYDHSYYFISSFIGEHIAYHANRLRLSA"
                     /name="fghA"
     gene            complement(7353..7751)
                     /locus_tag="B8O09_RS30725"
                     /old_locus_tag="B8O09_30675"
     CDS             complement(7353..7751)
                     /locus_tag="B8O09_RS30725"
                     /old_locus_tag="B8O09_30675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="WP_000235177.1"
                     /translation="MATPVISGNDVFSHVFIGASDVEKSADFYDAVLGTLGINNLGPFG
                     NGWVLYGREKPAFIIAHPGNGEAPSANGVTVGFAAASPEEVDAFHAAGLAAGGKDEGQP
                     GPRSHLPGAYAAYLRDPAGNKITAYTFI"
                     /name="VOC family protein"
     gene            complement(7793..8902)
                     /gene="frmA"
                     /locus_tag="B8O09_RS30730"
                     /old_locus_tag="B8O09_30680"
     CDS             complement(7793..8902)
                     /gene="frmA"
                     /locus_tag="B8O09_RS30730"
                     /old_locus_tag="B8O09_30680"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="WP_000842086.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKNTHTGVCHTDAF
                     TLSGNDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCQSGKTN
                     LCVSVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEANHEH
                     VCLLGCGVTTGIGAVHNTAKVQPGDSVAIFGLGAIGLAAVQGARQAKAGRIIAIDTNPA
                     KFELARQFGATDCINPNDHDKPIKDVLLEINKWGIDHTFECIGNVNVMRAALESAHRGW
                     GQSIVIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRTQLPAMVEDAMKGEIELEPF
                     VTHTMTLDEINEAFDLMHEGKSIRTVIRY"
                     /name="frmA"
     gene            complement(8933..9208)
                     /gene="frmR"
                     /locus_tag="B8O09_RS30735"
                     /old_locus_tag="B8O09_30685"
     CDS             complement(8933..9208)
                     /gene="frmR"
                     /locus_tag="B8O09_RS30735"
                     /old_locus_tag="B8O09_30685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional repressor
                     FrmR"
                     /protein_id="WP_001141270.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERALENGAECRSILQQIAAV
                     RGAANGLMAEVLESHIRETFDQNDSYSHEISKSVDDTIELVRAYLK"
                     /name="frmR"
     gene            9564..9725
                     /locus_tag="B8O09_RS30740"
                     /old_locus_tag="B8O09_30690"
     CDS             9564..9725
                     /locus_tag="B8O09_RS30740"
                     /old_locus_tag="B8O09_30690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_086893471.1"
                     /translation="MAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDAEHLFRTLTSR
                     LDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            9725..10126
                     /locus_tag="B8O09_RS30745"
                     /old_locus_tag="B8O09_30695"
     CDS             9725..10126
                     /locus_tag="B8O09_RS30745"
                     /old_locus_tag="B8O09_30695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_086893472.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSICSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            11026..11874
                     /locus_tag="B8O09_RS30750"
                     /old_locus_tag="B8O09_30700"
     CDS             11026..11874
                     /locus_tag="B8O09_RS30750"
                     /old_locus_tag="B8O09_30700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011346608.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="WP_225368678.1"
                     /translation="MFGEDLNFSKANNNCEIVIGDSREVIKTLGDKVFDCIVTSPPYWG
                     LRDYGVGNQIGAEINIDDYIFDLVSLFKEAKRTLKDDGTLWVNIGDSYTSGGRTWRDKD
                     DKNKGRAMSYRPPTPEGLKPKDLIGVPWKLAFALQQDGWYLRSDIIWNKPNCQPESVKD
                     RPTRSHEYIFLFSKNKKYHYDSDAIKEPSMDKKQGKKNRRTVWNINTEPYPGSHFAVYP
                     REMARLCVLAGSRPGGLVLDPFFGSGTTGVVCNSLGRKCVGIELNEDYAKLAKERILSG
                     R"
                     /name="site-specific DNA-methyltransferase"
     gene            complement(11863..12528)
                     /locus_tag="B8O09_RS30755"
                     /old_locus_tag="B8O09_30705"
     CDS             complement(11863..12528)
                     /locus_tag="B8O09_RS30755"
                     /old_locus_tag="B8O09_30705"
                     /inference="COORDINATES: protein motif:HMM:NF036585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_086893478.1"
                     /translation="MKAYEIEKIYVNHNKGYFNQKFIDNFGDRLITHYFNSNRPLTKTL
                     FEHAFNDSLNESGMQSQLAESRTNPGYDITIQNIKASLKTEAARNISQKNIHVSKWMEL
                     GKGKWVLEELLARFLAHLNNYDKIFTLRYIKPTYLTFKYQLIEIPKTLLLESKNAHLVV
                     MENSTQDPKPGYGYVFDQNGEKKFSLYFDGGSERKLQIKHLNVSLCIIHAEWCYTLPS"
                     /name="hypothetical protein"
     Typing          complement(1844..2251)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.355"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttaacattgg tgatagttat acatctggag gaagaacctg gagagataag gatgataaaa
       61 ataaaggtag ggctatgtca tataggcctc ctacacctga agggctaaag ccgaaggatt
      121 taattggcgt tccttggaag cttgcatttg ctttacagca agatggatgg tacctacgat
      181 ctgatattat atggaataaa ccaaattgcc agcctgaaag tgtaaaagat aggcctacaa
      241 ggtctcatga gtatatattt ctcttttcta aaaacaaaaa ataccattat gattccgatg
      301 ccataaaaga accatccatg gacaaaaaac aaggaaaaaa aatcggagga cagtttggaa
      361 tataaataca gagccttacc caggtagtca ttttgcagtt tatccaagag aaatggcaag
      421 gttatgtgtt ttagctggat caagacctgg aggtttggtt cttgatcctt tctttggttc
      481 tggtactaca ggtgttgtgt gtaattcctt gggtagaaaa tgcgttggga ttgagctaaa
      541 tgaagattac gctaagcttg ccaaagagcg tatcctatca ggaaggtagt gtgtaacacc
      601 attcagcgtg aattatgcac aaacttacat ttagatgttt tatttgaagt tttctctctg
      661 agccccatca aagtagagag aaaacttttt ttcaccattt tgatcaaaaa cgtaaccata
      721 tcctggtttt gggtcttgag tactattttc cattacaact agatgtgcat tttttgactc
      781 caaaagtagt gtttttggta tctcaataag ctgatattta aatgtaagat aggttggttt
      841 aatataccgg agtgtgaata ttttatcgta gttatttaaa tgcgctaaga atctcgctag
      901 taattcttct aaaacccact ttcctttacc aagttccatc catttgctta catgaatgtt
      961 tttttgactt atattcctgg ctgcctctgt ttttaaacta gctttaatgt tttgtatcgt
     1021 tatatcgtac cccggattag ttcgactttc agctagttga gattgcatcc cagattcgtt
     1081 taaagaatca ttgaaagcat gctcgaataa tgtcttggtt aatggtctgt tgctgttgaa
     1141 ataatgagtt attagcctgt cgccaaaatt atcaataaat ttttgattga aataaccttt
     1201 attgtggtta acatatattt tttcgatttc atacgctttc atccatttta agaaattatc
     1261 ttgctgcgtt tcatttagct gtctccatag cttaagtaat tcatgctctc tactcatctt
     1321 cttttttcct taataaatcc ggtaccgatg ttttaagcgc gctagcaata aaaattaaag
     1381 tgctgagggt tacatttctt tcctttcttt caactgaacc aatatatgta cggtggagtc
     1441 cagaaagctc cgctaaatct tcttgtgata aattattttt atttctatat cttttgatat
     1501 tttcagaaag tactgttttt atatttgaaa aatcatcatc aaagggttgc atttcttatt
     1561 tgcctgtaaa taaattaatt tataaatgca tgatgattat gagtcgacag actataagta
     1621 gcttttgttc tttaacgagc ttgaggatga aaagaaaatg aggtatgttc tgcttgtttt
     1681 aaaacgaaaa aaccgcctgg ggaggcggtt ttttcgaggg atcagtaagt tggggaactg
     1741 actgatccga ggtaactggc tagtagagga gcacagtcac caaatctcgt tctctcagtg
     1801 tagccttaac aggtacataa gttcaagact caactcctct aatcagttac cagtggctgc
     1861 tgccagtggc gcttttgcat gtcttaccgg gttggactca agatgatagt taccggataa
     1921 ggcgcagcag tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc
     1981 cttcccggaa ctgagtgtca ggcgtggaat gagataaacg cggccataac agcggaatga
     2041 caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccatca gggggaaacg
     2101 cctggtatct ttaaagtcct gtcgggtttc gccaccactg atttgagcgt caaattctgt
     2161 gatgcttgtc aggggggcgg agcctatgga aaaaccgccg cggtgcggcc tttccttttc
     2221 cccatcacgc gattgctcgg ctttcccgag cttttatacg catgctcctg ccgctcgccg
     2281 cagccgaacg gcaggagcgt agcgactgag tgagcgagga agcggaaaag agaacatccc
     2341 cacactgagc actgacgctc tgaggcctct tatgacgaaa caacagaaaa cggggtttgg
     2401 ggagtagtgg aacccaaaag acagttaagc ggttcaattg aatggtcaat aacaggtaaa
     2461 tcaatctctt gagttcaact gcgtaagttg ctctgcaaac ggccaagatg agtagagcca
     2521 atttggctac cgcaaacgag atagccaaaa gagtaaattc acgatgtaaa ttggccggtt
     2581 gatttttggc catttaaaat aaccgcttaa ggggtcagtt tggatatcga aaattattgt
     2641 acgttaaggc tattttttga cctttcgact ttagctggtc tcagccgacg aaacttaccg
     2701 gaagccggta tccggttgct tttccatata taatcgcctg tcagattgat gtgttcccag
     2761 cctaacgggg acaaatgcga cagcagttgc tcgttgatct tcaccccttt gcgcttcagg
     2821 gcatcgaccg ctctttccat atagacggta ttccacaggg atatggcagc cgtcagcagc
     2881 gtcagtccac tggcgcgata gctctgattt tccggttttc tgtccctgat ttcacccagc
     2941 cggtgtagga ataccgcacg cgccagcgcg ttacgggctt ctcctttatt cagtcccgcc
     3001 tgtacccgcc gacgaagtgc cggatcgcgg aaccagtcga gcataaacag agttcgttca
     3061 atacgaccaa tttccctcag tgctttggca agtccgtttt gtttggggta gctggcaagc
     3121 tttttcaaca tcagggatgc cgtcacggtt ccctgcttta ttgaagtggc cagacgaagt
     3181 acttcacgcc agtgaatttc gatctctttc agattcaggc tggtcgttga tatcagtgac
     3241 tgaagcgccg ggtattggtc tgctttccct ttgataaaca gctttttgtc gtggagatcc
     3301 ctgatccgcg gacagaacgc aaatcccagc agatgcatca gggcaaagac atgatcggtg
     3361 aaaccagcgg tatcggtgta atgctccctg atttccaggt cgctttcgtg gtacagcaag
     3421 ccatcgagca catgggttga atccctgacc cggctgatta tgcaggtgta aaacgggctg
     3481 tattgatccg aaatatgggt ataaaactga cgtccaggct cttgcccata tttcgggtta
     3541 acctgccctg cgtagcggcc tgagctgcct gttctgaaat tctgtccatc tgatgatgac
     3601 gttgtcccgt ctccccagaa tgccgccagt ggtcttttcc cctgcgcgtt taccagctct
     3661 gcgagggcgg ctgaataggt ttcgtcgcgg atgtaccatg cctggatatc ttccagtgat
     3721 gatttggtgc ttccggggca ggcttccgcc attttggtca ggccaagatt gatgccatcc
     3781 gcaaggatgg tggtgaggag caggcgggta tcgggacggg taatgtcatt ttttatatgc
     3841 gaaaaatgtc gggtaaacgc cgtccagctg tttacctcat cgagaatttc cgtgatttta
     3901 gggcgcggta gcatgctgta aaccagctct gccagcggtg aaacctgtac cgggacgcag
     3961 ttatccagcg gagagacttt tactcccctg tcagatattt cgacatcggg cagctcacca
     4021 agggcagcca tggcattgac ctcttcaagt ctggactgaa gcagagtctt acggctggta
     4081 atgtactcat gataatcagc agatacgggc aggggcagcg ccggtgtgag tttgtcaaag
     4141 tccttttcgg gaatgaggta atcatcaaag tttttgtagc ggcgtgagcc tttaacccag
     4201 atgtccccgg agcgcaatgc acccttaagc tcgctgagcg cacaaaattc atagtactgc
     4261 cggtcaatac ccgcaggtgt aatcaccact ttgcgccagc tttcagggac aaactcaagc
     4321 ggcgctgtcg ccggaacttt acgcgactgc ttccggtaca tatccttgat cacaaccagc
     4381 gcatcagcca gaggttgcgc tgccggggtt gcgattaact gtaaagcgga caacatgcgc
     4441 ggggcatatt tccgtaatgt gctgtatttc tcggtaataa tatgtaacgg gtcaaaatta
     4501 ttttttctgg caagatggcg tgtttcctcc agactggcga caaattcagg ccatgggagt
     4561 atgttttcta ttgcgagcca tggatcgcca ccggaatcac gagcttcaga cagtgcctgg
     4621 ccgacatcta tgtactgcct cagttttgac tggattagct ttccggtctg ttgaaggcgc
     4681 tcagcctgtg ttcttttggc tttgctgaac aggctgttca gcatgcgttc atgcagctca
     4741 atcacttcat ctgtcagtgt ggctctggct tcttccagca cgcacaccag gatcgcatga
     4801 cggcgggctg ctgaaaatcg ggtcaaatcc cggctactca tctttcggcc ttcccgcgcc
     4861 agtttcagca accggttctg gtgaatggta cgatctatac cttcaggtaa tgccagcgat
     4921 tcaatgctac tgagccgatc aaggtgttgc agcacgttct taccattgat tttacccggc
     4981 ggttgcagaa gccatgtcag cctggaaggc tgattatctg aggactcaag taaacgatcc
     5041 agggcatccc tgtgagccgg agttaacggg gcattcaggg tctgaaatac gattttatcg
     5101 ccgccagcca tggcctccgc acaggtacgc tccacgacat caatcgcggg aattatcacg
     5161 ttgttcttcc ggagccagac cagcagttct tcggccagga gaataccttt atcggtgcgc
     5221 gtcgccatcg gcagcaggtg tgtaatacag tcctgctgta tttttccggt aaacgctttt
     5281 acgccaagat agcggtacag ttcggttaaa tgttcgcgtc gcgtggtttc cctgccggaa
     5341 aggtaagcag gccagagatc cccggtaagt tttagcctgc tggcgatatg cttcagcagc
     5401 gcatcagagg gtgggatttt tttatccggt gcaaagccga catttttcag atagcaaaga
     5461 aggacggcaa aaccaaaacg actggcgggt ttacggtgag cactgataag cgccagatca
     5521 tgctcactaa aatacgccat tcgtgtcagc gttaactcat cgtccggtaa tgccagtaat
     5581 gattctcttt ccgacagaga aagaatctgc ctccttgcca tatagtttcc ctcataaatt
     5641 atttgatgcg gaatttttaa caaaaatggt atcgcataag ggtacaccct gacgccatat
     5701 tcaaaacgtg acggcacacg atgaatgaca taacctatat gcgatagatt aaatgcaata
     5761 acttaaatgc gataacgtgg tggaaaaatg ttcatcagag cttatttaag ggcatcgacg
     5821 gaagatcagt tcgccgatcg ggcaaaagag atgctggagc agtttgttca ggagcgggga
     5881 cataaaatag ccagttacta ccgggagaac atcagcggta caaaacttga ccgcccggaa
     5941 ctggggcgct tactgatgga cagtcaccgc aatgatattt tactggtcga gcagatagac
     6001 cgtctgaccc gtctgagcaa cagcgactgg atgacgctga aaaagcaaat cgagcaacac
     6061 gagctgcgga ttgtcagcct cgatgtaccc acctcatggc aggcgctgtc agataaagat
     6121 ccttcgcagg ctgacccaat cacccgcgcc gtgataaccg ccatcaataa tatgcttatc
     6181 gacctgatgg cctcgatgtc gcataaggac tggctgagcc gccgccatcg gcagaagcag
     6241 ggaattgaac gggcacatac gctgggaaaa taccggggca aacaggccga tcaggaacga
     6301 cataagaaag ttctgtatta ccgacaggtc aagaaactga gcataagaga aactgctgaa
     6361 gccaccggtt acagcacatc tcaggtctgc aggattcagg ccctgttcag gcctgaaaat
     6421 taagcacatt ttctgaggct ggaaatcaac tcatgctgac agcctgagcc tgttggcatg
     6481 gtaagcaatg tgctcaccaa taaagctgga aatgaaataa tagctgtggt cgtaaccagt
     6541 atgatatcgg aatgatgtgt tgatgttcat ctcgttgcac actctttcaa gacttttagt
     6601 gcgtaactga tcttcataaa aggcgtcact taatccctgg tcaataaaaa tttctggtaa
     6661 tttcgcaccc tgtaaaatga gactgacggg atcgtagtgc tcccacgttt ttttattttc
     6721 acccaggtac gcggtgaatg cctgttgtcc ccatggcacc tgagaaggtg acactattgg
     6781 tgaaaatgcc gatacactca catattcatc aggatttcgc agcgacagaa ccagcgctcc
     6841 cagccccccc atggagtgac cggatattga tttccttgat gttacaggta aatgctccat
     6901 gacaacatcc ggaagctcat ggagaatata gtcatacatc ctgtaatgcg tgttccacgg
     6961 atgttccgtc gcgttcagat aaaatccggc accctggccc agatcgtagc tgtcagcatc
     7021 tgcgacatgc tctccacggg gactggtatc aggaacaacg acgatgatgt tgttcctggc
     7081 ggcataatgc tgcatacccg atttggtgat gaaattctgc tccgtacagg tcagacccga
     7141 gagccagtac aggaccggca gtttttctgt tgctgctttg gggggaagat aaacaccgaa
     7201 attcatccca caattaagtg tttgtgaatt atggcggtat acgttttgcc agccgccagc
     7261 gctggcatgc tgctcgatga gttccatcag cctctgtctc atggttattt cccctgacag
     7321 ggagtcaggg gaagtctctt tatagccgga cgtcagatga acgtataagc cgtgattttg
     7381 ttacccgcag ggtctcgcag gtaggctgca taggctccag ggagatggct gcgcgggcca
     7441 ggctgacctt catctttccc tccggcggca aggcccgcgg cgtggaaagc atcaacttct
     7501 tccggacttg cagctgcaaa gccgacagta acgccgttgg ccgatggcgc ttcaccgttg
     7561 cccggatgtg caataataaa ggctggcttt tcgcgaccat aaagtaccca gccattgcca
     7621 aacgggccga ggttattgat gccaagcgta ccgaggacag catcgtaaaa atcagcggat
     7681 ttttcaacat cggatgcacc gatgaagacg tgggaaaaaa cgtcattacc agaaattaca
     7741 ggtgttgcca tgatccattt ccttcagttg gtttgactca gttaagacgc tatcagtaac
     7801 ggattaccgt acggattgat ttaccttcat gcataagatc gaaggcctca ttgatttcat
     7861 ccagcgtcat cgtgtgggta acaaatggct ccagttcaat ttcacccttc atggcatctt
     7921 ctaccatggc cggaagttgc gtacggcctt tcactccgcc gaaagcagaa cctttccata
     7981 cgcgcccggt aacgagctgg aatggacggg tggatatctc ctgcccggcg ccggccacgc
     8041 cgatgacaat ggactggccc cagccacggt gagcactttc cagcgccgca cgcataacgt
     8101 tgacgttacc gatacattca aaagtgtggt caatgcccca tttgttgatt tcaagcagca
     8161 catctttaat cggtttgtca tggtcgttcg ggttgatgca gtcggttgca ccgaactgac
     8221 gggcaagttc gaatttggcc gggttggtgt cgatagcgat aatgcgtcct gcttttgcct
     8281 ggcgagcccc ctgaacggca gccagaccga ttgcgccaag accaaaaata gcgacagaat
     8341 ctccaggctg tactttcgct gtgttgtgaa cagcgccaat cccggtagtc acgccacagc
     8401 ccagcaggca gacgtgttca tggtttgcct ccgggttaat ttttgccaga gaaacctcgg
     8461 ccactaccgt atactcactg aaggtggagc atcccatgta gtggtaaaga ggctggccat
     8521 tgtaagaaaa gcgggtggtt ccgtcaggca tcagtccttt tccctgagtt tcgcgaaccg
     8581 atacacagag gttcgttttc ccggactgac agaattcaca ctccccgcat tcggcggtgt
     8641 aaagcggaat cacatggtct cccggcttca cgctggtcac gccttcaccg acctccacga
     8701 cgacaccggc cccttcgtgg ccgagaacca ccgggaaaac gccctcagga tcgttcccgg
     8761 agagggtaaa ggcatcggtg tggcagacac cggtgtgggt atttttaatc agcacctcgc
     8821 cttttttagg tggtgcaaca tcaatttcaa caatctccag gggcttccct ggtgcaaatg
     8881 caacggcagc acgtgatttc atatgtaaat tatcctcaat gtgatggctt agctatttaa
     8941 gataggcgcg aaccagttca atcgtatcgt caacggattt actgatttca tggctgtagc
     9001 tgtcattctg gtcaaaggtt tcccgtatgt ggctctcgag cacctctgcc ataagaccgt
     9061 tagctgctcc gcgaacggca gctatctgct gaagaatgga acggcattca gcaccatttt
     9121 ccagcgctct ttccagagca tcaatctgac cccgaatgcg gcgaacacgg gtaaggacct
     9181 tttttttctc ttcaggagta ctgggcatta tttacctcac catttttgat tttatatact
     9241 atagtggagt atataattat cgtcaatgca tactccacta tagtataact aagaataaac
     9301 ataactacct ttatttacga tataaatcat atatttatat tatttttggc tgtgatactg
     9361 tcggaaggaa agttactaca aaaatgatat ctaccgatac gggatttggg gcataggcgg
     9421 ttctgttaaa tttcgccaga tattggctca tcattgatgg tcaaaaaatg agcctaacgt
     9481 acaataattt tcgatatcca aactgacccc cttaagggcg tttactgttc cgttgctccc
     9541 gaaaccccaa aacagcgccg aacatggcta aattcatcca gaatcagtct ctgctcctgc
     9601 tggagaagct taacgagctt gatctcgatg tcgaggccga cctttgtgag aaactccacg
     9661 atgatgcaga gcatcttttc cgtacactga cctcgagact ggatgcatta caggatggta
     9721 actgatggcc agagtgtcta ttagtgaggc tgcccgactg acgggtaaga gtagaacaac
     9781 actgcacagg ttaattaaag caggtgacct gtccatctgt agcggtgagc gtaatgcaaa
     9841 aatggtagat acttcagagc ttttacgtgt ctttggtccc tttgagcaac cgaagggtga
     9901 acaccattct ggacaggtct ctggacagcg tgatacagcc tctgctgacc agagtgaaca
     9961 ggtgattcgt catctgaaac aggagattga acacctgaac accttacttt tgtccaagga
    10021 ttcgcacatt gacagcctga aacaggccat gctccttctt gaaaatcacc aggcaaaatt
    10081 aacggaatcc ccggcgccat ggtggaaatt ctggaaaaaa tcctgacccc gtcatgcttc
    10141 agttttcctc tccggccatc ccggggagtt cctgaacctt cccaaaaatc gtcagatctc
    10201 tgaacgctcc tcacgactaa aaatacccac acactacgca aaattaaaat tttgcagatt
    10261 tgtatttaat atcagtagct tactttcctg aaatttcggt ttttaaattt tatttacatg
    10321 gatccgactc tatgacgagt cgtcatagag tccccggcga gtcgtcatag agtcggtaac
    10381 gactcagggc tataaaaaca atgagttaat aacgacccga ctctagaccc gactccccgt
    10441 ttggttgttt tttagactcg gcgactctag gtacgactct agtgaagagt cgccggtgaa
    10501 tcgtgtggtg agtcgtccgg cgacttgcca taacgtgatt gccggccgtc gacgaacccg
    10561 attcctgaac gtgctgaaaa ccggttttcc ggctgcgccg gcaacgcttc acggccacgc
    10621 aataaattgc gtccccgttc accgttttcg gatgggaaga acgccggaaa caggacttac
    10681 cctggctgaa tggggtgaat ttccgcgcta tgtacttgcg cgcatactca tgcatgccgt
    10741 aaaaacagag cctgcgcgtt tctggcgggt tttcgggtgg tttgttgcct gttttaccgg
    10801 tttcccgtca gaaacgccct gagggcctct caggcggtgc tgataacgga tgttatggta
    10861 aataagtcaa agtatgaggt tgctactact gttctgatgc tcttgatgaa tagatacgtt
    10921 gcttaagtca gcttgttgtc tttttatgtt tttttgaata ctataagtag catcagtcat
    10981 taaataaata gtgtgtatat gtacaaaaac tgcacagagt tagatttgtt tggagaagat
    11041 ttaaactttt ctaaagctaa taataattgc gaaatagtta ttggtgattc aagagaagta
    11101 ataaaaacac taggtgataa ggtgtttgat tgcatcgtaa cttcacctcc atactggggc
    11161 ttgcgagatt atggtgttgg aaatcaaata ggtgctgaga taaatattga tgattatata
    11221 tttgatcttg tctctctttt taaagaagca aaaagaacat taaaagacga tggtactctt
    11281 tgggttaaca ttggtgatag ttatacatct ggaggaagaa cctggagaga taaggatgat
    11341 aaaaataaag gtagggctat gtcatatagg cctcctacac ctgaagggct aaagccgaag
    11401 gatttaattg gcgttccttg gaagcttgca tttgctttac agcaagatgg atggtaccta
    11461 cgatctgata ttatatggaa taaaccaaat tgccagcctg aaagtgtaaa agataggcct
    11521 acaaggtctc atgagtatat atttctcttt tctaaaaaca aaaaatacca ttatgattcc
    11581 gatgccataa aagaaccatc catggacaaa aaacaaggaa aaaaaaatcg gaggacagtt
    11641 tggaatataa atacagagcc ttacccaggt agtcattttg cagtttatcc aagagaaatg
    11701 gcaaggttat gtgttttagc tggatcaaga cctggaggtt tggttcttga tcctttcttt
    11761 ggttctggta ctacaggtgt tgtgtgtaat tccttgggta gaaaatgcgt tgggattgag
    11821 ctaaatgaag attacgctaa gcttgccaaa gagcgtatcc tatcaggaag gtagtgtgta
    11881 acaccattca gcgtgaatta tgcacaaact tacatttaga tgttttattt gaagttttct
    11941 ctctgagccc ccatcaaagt agagagaaaa ctttttttca ccattttgat caaaaacgta
    12001 accatatcct ggttttgggt cttgagtact attttccatt acaactagat gtgcattttt
    12061 tgactccaaa agtagtgttt ttggtatctc aataagctga tatttaaatg taagataggt
    12121 tggtttaata taccggagtg tgaatatttt atcgtagtta tttaaatgcg ctaagaatct
    12181 cgctagtaat tcttctaaaa cccactttcc tttaccaagt tccatccatt tgcttacatg
    12241 aatgtttttt tgacttatat tcctggctgc ctctgttttt aaactagctt taatgttttg
    12301 tatcgttata tcgtaccccg gattagttcg actttcagct agttgagatt gcatcccaga
    12361 ttcgtttaaa gaatcattga aagcatgctc gaataatgtc ttggttaatg gtctgttgct
    12421 gttgaaataa tgagttatta gcctgtcgcc aaaattatca ataaattttt gattgaaata
    12481 acctttattg tggttaacat atattttttc gatttcatac gctttcatcc attttaagaa
//
