LOCUS       NZ_CP020845             9510 bp    DNA     circular CON 25-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KPN1482 plasmid pKPN1482-5, complete
            sequence.
ACCESSION   NZ_CP020845
VERSION     NZ_CP020845.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06438459
            Assembly: GCF_002156745.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Long,S.W., Olsen,R.J., Eagar,T.N., Beres,S.B., Zhao,P., Davis,J.,
            Brettin,T., Xia,F. and Musser,J.M.
  TITLE     Population Genomic Analysis of 1,777 Extended-Spectrum
            Beta-Lactamase Producing Klebsiella pneumoniae, Houston, Texas:
            Unexpected Abundance of Clonal Group 307
  JOURNAL   MBio (2017) In press
REFERENCE   2  (bases 1 to 9510)
  AUTHORS   Long,S.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2017) Pathology & Genomic Medicine, Houston
            Methodist Hospital, 6565 Fannin St, Houston, TX 77030, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 201x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2023 02:43:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,021
            CDSs (total)                      :: 5,894
            Genes (coding)                    :: 5,710
            CDSs (with protein)               :: 5,710
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 89 of 184
            Pseudo Genes (incomplete)         :: 95 of 184
            Pseudo Genes (internal stop)      :: 19 of 184
            Pseudo Genes (multiple problems)  :: 19 of 184
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020845.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9510
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN1482"
                     /isolation_source="respiratory"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPN1482-5"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7107 N 95.4006 W"
                     /collection_date="2014-06-11"
                     /collected_by="Houston Methodist Hospital"
                     /genotype="ST37; Capsule locus KL105"
     gene            join(8934..9510,1..8)
                     /locus_tag="B8O09_RS30635"
                     /old_locus_tag="B8O09_30590"
     CDS             join(8934..9510,1..8)
                     /locus_tag="B8O09_RS30635"
                     /old_locus_tag="B8O09_30590"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_086893476.1"
                     /translation="MFIRAYLRASTEDQFADRAKEMLEQFVQERGHKIASYYRENISGT
                     KLDRPELGRLLMDSHRNDILLVEQIDRLTRLSNSDWMTLKKQIEQHELRIVSLDVPTSW
                     QALSDKDPSQADPITRAVITAINNMLIDLMASMSHKDWLSRRHRQKQGIERAHTLGKYR
                     GKQADQERHKKVLYYRQVKKLSIRETAEAIC"
                     /name="recombinase family protein"
     gene            complement(join(<9508..9510,1..762))
                     /locus_tag="B8O09_RS31140"
                     /old_locus_tag="B8O09_30595"
                     /pseudo=""
     CDS             complement(join(<9508..9510,1..762))
                     /locus_tag="B8O09_RS31140"
                     /old_locus_tag="B8O09_30595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic domain-containing
                     protein"
                     /name="alcohol dehydrogenase catalytic domain-containing
                     protein"
     gene            complement(793..1068)
                     /gene="frmR"
                     /locus_tag="B8O09_RS30640"
                     /old_locus_tag="B8O09_30600"
     CDS             complement(793..1068)
                     /gene="frmR"
                     /locus_tag="B8O09_RS30640"
                     /old_locus_tag="B8O09_30600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional repressor
                     FrmR"
                     /protein_id="WP_001141270.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERALENGAECRSILQQIAAV
                     RGAANGLMAEVLESHIRETFDQNDSYSHEISKSVDDTIELVRAYLK"
                     /name="frmR"
     gene            1424..1585
                     /locus_tag="B8O09_RS30645"
                     /old_locus_tag="B8O09_30605"
     CDS             1424..1585
                     /locus_tag="B8O09_RS30645"
                     /old_locus_tag="B8O09_30605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_086893471.1"
                     /translation="MAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDAEHLFRTLTSR
                     LDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1585..1986
                     /locus_tag="B8O09_RS30650"
                     /old_locus_tag="B8O09_30610"
     CDS             1585..1986
                     /locus_tag="B8O09_RS30650"
                     /old_locus_tag="B8O09_30610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_086893472.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSICSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            2886..3734
                     /locus_tag="B8O09_RS30655"
                     /old_locus_tag="B8O09_30615"
     CDS             2886..3734
                     /locus_tag="B8O09_RS30655"
                     /old_locus_tag="B8O09_30615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011346608.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="WP_225368678.1"
                     /translation="MFGEDLNFSKANNNCEIVIGDSREVIKTLGDKVFDCIVTSPPYWG
                     LRDYGVGNQIGAEINIDDYIFDLVSLFKEAKRTLKDDGTLWVNIGDSYTSGGRTWRDKD
                     DKNKGRAMSYRPPTPEGLKPKDLIGVPWKLAFALQQDGWYLRSDIIWNKPNCQPESVKD
                     RPTRSHEYIFLFSKNKKYHYDSDAIKEPSMDKKQGKKNRRTVWNINTEPYPGSHFAVYP
                     REMARLCVLAGSRPGGLVLDPFFGSGTTGVVCNSLGRKCVGIELNEDYAKLAKERILSG
                     R"
                     /name="site-specific DNA-methyltransferase"
     gene            complement(3723..4463)
                     /locus_tag="B8O09_RS30660"
                     /old_locus_tag="B8O09_30620"
     CDS             complement(3723..4463)
                     /locus_tag="B8O09_RS30660"
                     /old_locus_tag="B8O09_30620"
                     /inference="COORDINATES: protein motif:HMM:NF036585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_086893474.1"
                     /translation="MSREHELLKLWRQLNETQQDNFLKWMKAYEIEKIYVNHNKGYFNQ
                     KFIDNFGDRLITHYFNSNRPLTKTLFEHAFNDSLNESGMQSQLAESRTNPGYDITIQNI
                     KASLKTEAARNISQKNIHVSKWMELGKGKWVLEELLARFLAHLNNYDKIFTLRYIKPTY
                     LTFKYQLIEIPKTLLLESKNAHLVVMENSTQDPKPGYGYVFDQNGEKKFSLYFDGGSER
                     KLQIKHLNVSLCIIHAEWCYTLPS"
                     /name="hypothetical protein"
     gene            complement(4456..4698)
                     /locus_tag="B8O09_RS30665"
                     /old_locus_tag="B8O09_30625"
     CDS             complement(4456..4698)
                     /locus_tag="B8O09_RS30665"
                     /old_locus_tag="B8O09_30625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012575772.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_086893475.1"
                     /translation="MQPFDDDFSNIKTVLSENIKRYRNKNNLSQEDLAELSGLHRTYIG
                     SVERKERNVTLSTLIFIASALKTSVPDLLRKKEDE"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(5780..8767)
                     /locus_tag="B8O09_RS30680"
                     /old_locus_tag="B8O09_30635"
     CDS             complement(5780..8767)
                     /locus_tag="B8O09_RS30680"
                     /old_locus_tag="B8O09_30635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241615.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_032723026.1"
                     /translation="MARRQILSLSERESLLALPDDELTLTRMAYFSEHDLALISAHRKP
                     ASRFGFAVLLCYLKNVGFAPDKKIPPSDALLKHIASRLKLTGDLWPAYLSGRETTRREH
                     LTELYRYLGVKAFTGKIQQDCITHLLPMATRTDKGILLAEELLVWLRKNNVIIPAIDVV
                     ERTCAEAMAGGDKIVFQTLNAPLTPAHRDALDRLLESSDNQPSRLTWLLQPPGKINGKN
                     VLQHLDRLSSIESLALPEGIDRTIHQNRLLKLAREGRKMSSRDLTRFSAARRHAILVCV
                     LEEARATLTDEVIELHERMLNSLFSKAKRTQAERLQQTGKLIQSKLRQYIDVGQALSEA
                     RDSGGDPWLAIENILPWPEFVASLEETRHLARKNNFDPLHIITEKYSTLRKYAPRMLSA
                     LQLIATPAAQPLADALVVIKDMYRKQSRKVPATAPLEFVPESWRKVVITPAGIDRQYYE
                     FCALSELKGALRSGDIWVKGSRRYKNFDDYLIPEKDFDKLTPALPLPVSADYHEYITSR
                     KTLLQSRLEEVNAMAALGELPDVEISDRGVKVSPLDNCVPVQVSPLAELVYSMLPRPKI
                     TEILDEVNSWTAFTRHFSHIKNDITRPDTRLLLTTILADGINLGLTKMAEACPGSTKSS
                     LEDIQAWYIRDETYSAALAELVNAQGKRPLAAFWGDGTTSSSDGQNFRTGSSGRYAGQV
                     NPKYGQEPGRQFYTHISDQYSPFYTCIISRVRDSTHVLDGLLYHESDLEIREHYTDTAG
                     FTDHVFALMHLLGFAFCPRIRDLHDKKLFIKGKADQYPALQSLISTTSLNLKEIEIHWR
                     EVLRLATSIKQGTVTASLMLKKLASYPKQNGLAKALREIGRIERTLFMLDWFRDPALRR
                     RVQAGLNKGEARNALARAVFLHRLGEIRDRKPENQSYRASGLTLLTAAISLWNTVYMER
                     AVDALKRKGVKINEQLLSHLSPLGWEHINLTGDYIWKSNRIPASGKFRRLRPAKVERSK
                     NSLNVQ"
                     /name="Tn3 family transposase"
     Typing          complement(4990..5397)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.355"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tttgttgatt tcaagcagca catctttaat cggtttgtca tggtcgttcg ggttgatgca
       61 gtcggttgca ccgaactgac gggcaagttc gaatttggcc gggttggtgt cgatagcgat
      121 aatgcgtcct gcttttgcct ggcgagcccc ctgaacggca gccagaccga ttgcgccaag
      181 accaaaaata gcgacagaat ctccaggctg tactttcgct gtgttgtgaa cagcgccaat
      241 cccggtagtc acgccacagc ccagcaggca gacgtgttca tggtttgcct ccgggttaat
      301 ttttgccaga gaaacctcgg ccactaccgt atactcactg aaggtggagc atcccatgta
      361 gtggtaaaga ggctggccat tgtaagaaaa gcgggtggtt ccgtcaggca tcagtccttt
      421 tccctgagtt tcgcgaaccg atacacagag gttcgttttc ccggactgac agaattcaca
      481 ctccccgcat tcggcggtgt aaagcggaat cacatggtct cccggcttca cgctggtcac
      541 gccttcaccg acctccacga cgacaccggc cccttcgtgg ccgagaacca ccgggaaaac
      601 gccctcagga tcgttcccgg agagggtaaa ggcatcggtg tggcagacac cggtgtgggt
      661 atttttaatc agcacctcgc cttttttagg tggtgcaaca tcaatttcaa caatctccag
      721 gggcttccct ggtgcaaatg caacggcagc acgtgatttc atatgtaaat tatcctcaat
      781 gtgatggctt agctatttaa gataggcgcg aaccagttca atcgtatcgt caacggattt
      841 actgatttca tggctgtagc tgtcattctg gtcaaaggtt tcccgtatgt ggctctcgag
      901 cacctctgcc ataagaccgt tagctgctcc gcgaacggca gctatctgct gaagaatgga
      961 acggcattca gcaccatttt ccagcgctct ttccagagca tcaatctgac cccgaatgcg
     1021 gcgaacacgg gtaaggacct tttttttctc ttcaggagta ctgggcatta tttacctcac
     1081 catttttgat tttatatact atagtggagt atataattat cgtcaatgca tactccacta
     1141 tagtataact aagaataaac ataactacct ttatttacga tataaatcat atatttatat
     1201 tatttttggc tgtgatactg tcggaaggaa agttactaca aaaatgatat ctaccgatac
     1261 gggatttggg gcataggcgg ttctgttaaa tttcgccaga tattggctca tcattgatgg
     1321 tcaaaaaatg agcctaacgt acaataattt tcgatatcca aactgacccc cttaagggcg
     1381 tttactgttc cgttgctccc gaaaccccaa aacagcgccg aacatggcta aattcatcca
     1441 gaatcagtct ctgctcctgc tggagaagct taacgagctt gatctcgatg tcgaggccga
     1501 cctttgtgag aaactccacg atgatgcaga gcatcttttc cgtacactga cctcgagact
     1561 ggatgcatta caggatggta actgatggcc agagtgtcta ttagtgaggc tgcccgactg
     1621 acgggtaaga gtagaacaac actgcacagg ttaattaaag caggtgacct gtccatctgt
     1681 agcggtgagc gtaatgcaaa aatggtagat acttcagagc ttttacgtgt ctttggtccc
     1741 tttgagcaac cgaagggtga acaccattct ggacaggtct ctggacagcg tgatacagcc
     1801 tctgctgacc agagtgaaca ggtgattcgt catctgaaac aggagattga acacctgaac
     1861 accttacttt tgtccaagga ttcgcacatt gacagcctga aacaggccat gctccttctt
     1921 gaaaatcacc aggcaaaatt aacggaatcc ccggcgccat ggtggaaatt ctggaaaaaa
     1981 tcctgacccc gtcatgcttc agttttcctc tccggccatc ccggggagtt cctgaacctt
     2041 cccaaaaatc gtcagatctc tgaacgctcc tcacgactaa aaatacccac acactacgca
     2101 aaattaaaat tttgcagatt tgtatttaat atcagtagct tactttcctg aaatttcggt
     2161 ttttaaattt tatttacatg gatccgactc tatgacgagt cgtcatagag tccccggcga
     2221 gtcgtcatag agtcggtaac gactcagggc tataaaaaca atgagttaat aacgacccga
     2281 ctctagaccc gactccccgt ttggttgttt tttagactcg gcgactctag gtacgactct
     2341 agtgaagagt cgccggtgaa tcgtgtggtg agtcgtccgg cgacttgcca taacgtgatt
     2401 gccggccgtc gacgaacccg attcctgaac gtgctgaaaa ccggttttcc ggctgcgccg
     2461 gcaacgcttc acggccacgc aataaattgc gtccccgttc accgttttcg gatgggaaga
     2521 acgccggaaa caggacttac cctggctgaa tggggtgaat ttccgcgcta tgtacttgcg
     2581 cgcatactca tgcatgccgt aaaaacagag cctgcgcgtt tctggcgggt tttcgggtgg
     2641 tttgttgcct gttttaccgg tttcccgtca gaaacgccct gagggcctct caggcggtgc
     2701 tgataacgga tgttatggta aataagtcaa agtatgaggt tgctactact gttctgatgc
     2761 tcttgatgaa tagatacgtt gcttaagtca gcttgttgtc tttttatgtt tttttgaata
     2821 ctataagtag catcagtcat taaataaata gtgtgtatat gtacaaaaac tgcacagagt
     2881 tagatttgtt tggagaagat ttaaactttt ctaaagctaa taataattgc gaaatagtta
     2941 ttggtgattc aagagaagta ataaaaacac taggtgataa ggtgtttgat tgcatcgtaa
     3001 cttcacctcc atactggggc ttgcgagatt atggtgttgg aaatcaaata ggtgctgaga
     3061 taaatattga tgattatata tttgatcttg tctctctttt taaagaagca aaaagaacat
     3121 taaaagacga tggtactctt tgggttaaca ttggtgatag ttatacatct ggaggaagaa
     3181 cctggagaga taaggatgat aaaaataaag gtagggctat gtcatatagg cctcctacac
     3241 ctgaagggct aaagccgaag gatttaattg gcgttccttg gaagcttgca tttgctttac
     3301 agcaagatgg atggtaccta cgatctgata ttatatggaa taaaccaaat tgccagcctg
     3361 aaagtgtaaa agataggcct acaaggtctc atgagtatat atttctcttt tctaaaaaca
     3421 aaaaatacca ttatgattcc gatgccataa aagaaccatc catggacaaa aaacaaggaa
     3481 aaaaaaatcg gaggacagtt tggaatataa atacagagcc ttacccaggt agtcattttg
     3541 cagtttatcc aagagaaatg gcaaggttat gtgttttagc tggatcaaga cctggaggtt
     3601 tggttcttga tcctttcttt ggttctggta ctacaggtgt tgtgtgtaat tccttgggta
     3661 gaaaatgcgt tgggattgag ctaaatgaag attacgctaa gcttgccaaa gagcgtatcc
     3721 tatcaggaag gtagtgtgta acaccattca gcgtgaatta tgcacaaact tacatttaga
     3781 tgttttattt gaagttttct ctctgagccc ccatcaaagt agagagaaaa ctttttttca
     3841 ccattttgat caaaaacgta accatatcct ggttttgggt cttgagtact attttccatt
     3901 acaactagat gtgcattttt tgactccaaa agtagtgttt ttggtatctc aataagctga
     3961 tatttaaatg taagataggt tggtttaata taccggagtg tgaatatttt atcgtagtta
     4021 tttaaatgcg ctaagaatct cgctagtaat tcttctaaaa cccactttcc tttaccaagt
     4081 tccatccatt tgcttacatg aatgtttttt tgacttatat tcctggctgc ctctgttttt
     4141 aaactagctt taatgttttg tatcgttata tcgtaccccg gattagttcg actttcagct
     4201 agttgagatt gcatcccaga ttcgtttaaa gaatcattga aagcatgctc gaataatgtc
     4261 ttggttaatg gtctgttgct gttgaaataa tgagttatta gcctgtcgcc aaaattatca
     4321 ataaattttt gattgaaata acctttattg tggttaacat atattttttc gatttcatac
     4381 gctttcatcc attttaagaa attatcttgc tgcgtttcat ttagctgtct ccatagctta
     4441 agtaattcat gctctctact catcttcttt tttccttaat aaatccggta ccgatgtttt
     4501 aagcgcgcta gcaataaaaa ttaaagtgct gagggttaca tttctttcct ttctttcaac
     4561 tgaaccaata tatgtacggt ggagtccaga aagctccgct aaatcttctt gtgataaatt
     4621 atttttattt ctatatcttt tgatattttc agaaagtact gtttttatat ttgaaaaatc
     4681 atcatcaaag ggttgcattt cttatttgcc tgtaaataaa ttaatttata aatgcatgat
     4741 gattatgagt cgacagacta taagtagctt ttgttcttta acgagcttga ggatgaaaag
     4801 aaaatgaggt atgttctgct tgttttaaaa cgaaaaaacc gcctggggag gcggtttttt
     4861 cgagggatca gtaagttggg gaactgactg atccgaggta actggctagt agaggagcac
     4921 agtcaccaaa tctcgttctc tcagtgtagc cttaacaggt acataagttc aagactcaac
     4981 tcctctaatc agttaccagt ggctgctgcc agtggcgctt ttgcatgtct taccgggttg
     5041 gactcaagat gatagttacc ggataaggcg cagcagtcgg actgaacggg gggttcgtgc
     5101 atacagtcca gcttggagcg aactgccttc ccggaactga gtgtcaggcg tggaatgaga
     5161 taaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg
     5221 cacgagggag ccatcagggg gaaacgcctg gtatctttaa agtcctgtcg ggtttcgcca
     5281 ccactgattt gagcgtcaaa ttctgtgatg cttgtcaggg gggcggagcc tatggaaaaa
     5341 ccgccgcggt gcggcctttc cttttcccca tcacgcgatt gctcggcttt cccgagcttt
     5401 tatacgcatg ctcctgccgc tcgccgcagc cgaacggcag gagcgtagcg actgagtgag
     5461 cgaggaagcg gaaaagagaa catccccaca ctgagcactg acgctctgag gcctcttatg
     5521 acgaaacaac agaaaacggg gtttggggag tagtggaacc caaaagacag ttaagcggtt
     5581 caattgaatg gtcaataaca ggtaaatcaa tctcttgagt tcaactgcgt aagttgctct
     5641 gcaaacggcc aagatgagta gagccaattt ggctaccgca aacgagatag ccaaaagagt
     5701 aaattcacga tgtaaattgg ccggttgatt tttggccatt taaaataacc gcttaagggg
     5761 tcagtttgga tatcgaaaat tattgtacgt taaggctatt ttttgacctt tcgactttag
     5821 ctggtctcag ccgacgaaac ttaccggaag ccggtatccg gttgcttttc catatataat
     5881 cgcctgtcag attgatgtgt tcccagccta acggggacaa atgcgacagc agttgctcgt
     5941 tgatcttcac ccctttgcgc ttcagggcat cgaccgctct ttccatatag acggtattcc
     6001 acagggatat ggcagccgtc agcagcgtca gtccactggc gcgatagctc tgattttccg
     6061 gttttctgtc cctgatttca cccagccggt gtaggaatac cgcacgcgcc agcgcgttac
     6121 gggcttctcc tttattcagt cccgcctgta cccgccgacg aagtgccgga tcgcggaacc
     6181 agtcgagcat aaacagagtt cgttcaatac gaccaatttc cctcagtgct ttggcaagtc
     6241 cgttttgttt ggggtagctg gcaagctttt tcaacatcag ggatgccgtc acggttccct
     6301 gctttattga agtggccaga cgaagtactt cacgccagtg aatttcgatc tctttcagat
     6361 tcaggctggt cgttgatatc agtgactgaa gcgccgggta ttggtctgct ttccctttga
     6421 taaacagctt tttgtcgtgg agatccctga tccgcggaca gaacgcaaat cccagcagat
     6481 gcatcagggc aaagacatga tcggtgaaac cagcggtatc ggtgtaatgc tccctgattt
     6541 ccaggtcgct ttcgtggtac agcaagccat cgagcacatg ggttgaatcc ctgacccggc
     6601 tgattatgca ggtgtaaaac gggctgtatt gatccgaaat atgggtataa aactgacgtc
     6661 caggctcttg cccatatttc gggttaacct gccctgcgta gcggcctgag ctgcctgttc
     6721 tgaaattctg tccatctgat gatgacgttg tcccgtctcc ccagaatgcc gccagtggtc
     6781 ttttcccctg cgcgtttacc agctctgcga gggcggctga ataggtttcg tcgcggatgt
     6841 accatgcctg gatatcttcc agtgatgatt tggtgcttcc ggggcaggct tccgccattt
     6901 tggtcaggcc aagattgatg ccatccgcaa ggatggtggt gaggagcagg cgggtatcgg
     6961 gacgggtaat gtcatttttt atatgcgaaa aatgtcgggt aaacgccgtc cagctgttta
     7021 cctcatcgag aatttccgtg attttagggc gcggtagcat gctgtaaacc agctctgcca
     7081 gcggtgaaac ctgtaccggg acgcagttat ccagcggaga gacttttact cccctgtcag
     7141 atatttcgac atcgggcagc tcaccaaggg cagccatggc attgacctct tcaagtctgg
     7201 actgaagcag agtcttacgg ctggtaatgt actcatgata atcagcagat acgggcaggg
     7261 gcagcgccgg tgtgagtttg tcaaagtcct tttcgggaat gaggtaatca tcaaagtttt
     7321 tgtagcggcg tgagccttta acccagatgt ccccggagcg caatgcaccc ttaagctcgc
     7381 tgagcgcaca aaattcatag tactgccggt caatacccgc aggtgtaatc accactttgc
     7441 gccagctttc agggacaaac tcaagcggcg ctgtcgccgg aactttacgc gactgcttcc
     7501 ggtacatatc cttgatcaca accagcgcat cagccagagg ttgcgctgcc ggggttgcga
     7561 ttaactgtaa agcggacaac atgcgcgggg catatttccg taatgtgctg tatttctcgg
     7621 taataatatg taacgggtca aaattatttt ttctggcaag atggcgtgtt tcctccagac
     7681 tggcgacaaa ttcaggccat gggagtatgt tttctattgc gagccatgga tcgccaccgg
     7741 aatcacgagc ttcagacagt gcctggccga catctatgta ctgcctcagt tttgactgga
     7801 ttagctttcc ggtctgttga aggcgctcag cctgtgttct tttggctttg ctgaacaggc
     7861 tgttcagcat gcgttcatgc agctcaatca cttcatctgt cagtgtggct ctggcttctt
     7921 ccagcacgca caccaggatc gcatgacggc gggctgctga aaatcgggtc aaatcccggc
     7981 tactcatctt tcggccttcc cgcgccagtt tcagcaaccg gttctggtga atggtacgat
     8041 ctataccttc aggtaatgcc agcgattcaa tgctactgag ccgatcaagg tgttgcagca
     8101 cgttcttacc attgatttta cccggcggtt gcagaagcca tgtcagcctg gaaggctgat
     8161 tatctgagga ctcaagtaaa cgatccaggg catccctgtg agccggagtt aacggggcat
     8221 tcagggtctg aaatacgatt ttatcgccgc cagccatggc ctccgcacag gtacgctcca
     8281 cgacatcaat cgcgggaatt atcacgttgt tcttccggag ccagaccagc agttcttcgg
     8341 ccaggagaat acctttatcg gtgcgcgtcg ccatcggcag caggtgtgta atacagtcct
     8401 gctgtatttt tccggtaaac gcttttacgc caagatagcg gtacagttcg gttaaatgtt
     8461 cgcgtcgcgt ggtttccctg ccggaaaggt aagcaggcca gagatccccg gtaagtttta
     8521 gcctgctggc gatatgcttc agcagcgcat cagagggtgg gattttttta tccggtgcaa
     8581 agccgacatt tttcagatag caaagaagga cggcaaaacc aaaacgactg gcgggtttac
     8641 ggtgagcact gataagcgcc agatcatgct cactaaaata cgccattcgt gtcagcgtta
     8701 actcatcgtc cggtaatgcc agtaatgatt ctctttccga cagagaaaga atctgcctcc
     8761 ttgccatata gtttccctca taaattattt gatgcggaat ttttaacaaa aatggtatcg
     8821 cataagggta caccctgacg ccatattcaa aacgtgacgg cacacgatga atgacataac
     8881 ctatatgcga tagattaaat gcaataactt aaatgcgata acgtggtgga aaaatgttca
     8941 tcagagctta tttaagggca tcgacggaag atcagttcgc cgatcgggca aaagagatgc
     9001 tggagcagtt tgttcaggag cggggacata aaatagccag ttactaccgg gagaacatca
     9061 gcggtacaaa acttgaccgc ccggaactgg ggcgcttact gatggacagt caccgcaatg
     9121 atattttact ggtcgagcag atagaccgtc tgacccgtct gagcaacagc gactggatga
     9181 cgctgaaaaa gcaaatcgag caacacgagc tgcggattgt cagcctcgat gtacccacct
     9241 catggcaggc gctgtcagat aaagatcctt cgcaggctga cccaatcacc cgcgccgtga
     9301 taaccgccat caataatatg cttatcgacc tgatggcctc gatgtcgcat aaggactggc
     9361 tgagccgccg ccatcggcag aagcagggaa ttgaacgggc acatacgctg ggaaaatacc
     9421 ggggcaaaca ggccgatcag gaacgacata agaaagttct gtattaccga caggtcaaga
     9481 aactgagcat aagagaaact gctgaagcca
//
