LOCUS       NZ_CP020840            11984 bp    DNA     circular CON 25-NOV-2023
DEFINITION  Klebsiella pneumoniae strain BK13043 plasmid pBK13043-3, complete
            sequence.
ACCESSION   NZ_CP020840
VERSION     NZ_CP020840.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06701573
            Assembly: GCF_002156725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11984)
  AUTHORS   Long,S.W., Olsen,R.J., Eagar,T.N., Beres,S.B., Zhao,P., Davis,J.,
            Brettin,T., Xia,F. and Musser,J.M.
  TITLE     Population Genomic Analysis of 1,777 Extended-Spectrum
            Beta-Lactamase Producing Klebsiella pneumoniae, Houston, Texas:
            Unexpected Abundance of Clonal Group 307
  JOURNAL   MBio (2017) In press
REFERENCE   2  (bases 1 to 11984)
  AUTHORS   Long,S.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2017) Pathology & Genomic Medicine, Houston
            Methodist Hospital, 6565 Fannin St, Houston, TX 77030, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 121x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2023 02:42:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,865
            CDSs (total)                      :: 5,741
            Genes (coding)                    :: 5,588
            CDSs (with protein)               :: 5,588
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 85 of 153
            Pseudo Genes (incomplete)         :: 81 of 153
            Pseudo Genes (internal stop)      :: 17 of 153
            Pseudo Genes (multiple problems)  :: 26 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020840.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11984
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BK13043"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pBK13043-3"
                     /geo_loc_name="USA: New York, New York City"
                     /lat_lon="40 N 74.5 W"
                     /collection_date="2004"
     gene            complement(join(11835..11984,1..>18))
                     /locus_tag="B9G35_RS31460"
                     /pseudo=""
     CDS             complement(join(11835..11984,1..>18))
                     /locus_tag="B9G35_RS31460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(<22..237)
                     /locus_tag="B9G35_RS31455"
                     /pseudo=""
     CDS             complement(<22..237)
                     /locus_tag="B9G35_RS31455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /GO_function="GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing protein"
                     /name="nucleotidyltransferase domain-containing protein"
     gene            complement(307..861)
                     /gene="aac(6')-Ib"
                     /locus_tag="B9G35_RS29960"
                     /old_locus_tag="B9G35_29965"
     ARG             complement(311..861)
                     /name="aac(6')-Ib"
                     /gene="aac(6')-Ib"
                     /gene_name="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1095..1652)
                     /locus_tag="B9G35_RS29965"
                     /old_locus_tag="B9G35_29970"
     CDS             complement(1095..1652)
                     /locus_tag="B9G35_RS29965"
                     /old_locus_tag="B9G35_29970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001217881.1"
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            1814..4819
                     /locus_tag="B9G35_RS29970"
                     /old_locus_tag="B9G35_29975"
     CDS             1814..4819
                     /locus_tag="B9G35_RS29970"
                     /old_locus_tag="B9G35_29975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143775.1"
                     /translation="MPVDFLTTEQVESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHDEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISQSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            4896..5315
                     /locus_tag="B9G35_RS29975"
                     /old_locus_tag="B9G35_29980"
     CDS             4896..5315
                     /locus_tag="B9G35_RS29975"
                     /old_locus_tag="B9G35_29980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            5621..5884
                     /locus_tag="B9G35_RS29980"
                     /old_locus_tag="B9G35_29985"
     CDS             5621..5884
                     /locus_tag="B9G35_RS29980"
                     /old_locus_tag="B9G35_29985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            5874..6173
                     /locus_tag="B9G35_RS29985"
                     /old_locus_tag="B9G35_29990"
     CDS             5874..6173
                     /locus_tag="B9G35_RS29985"
                     /old_locus_tag="B9G35_29990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6235..6612)
                     /locus_tag="B9G35_RS29990"
                     /old_locus_tag="B9G35_29995"
     CDS             complement(6235..6612)
                     /locus_tag="B9G35_RS29990"
                     /old_locus_tag="B9G35_29995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            6681..6860
                     /locus_tag="B9G35_RS29995"
                     /old_locus_tag="B9G35_30000"
     CDS             6681..6860
                     /locus_tag="B9G35_RS29995"
                     /old_locus_tag="B9G35_30000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6888..7418)
                     /locus_tag="B9G35_RS30000"
                     /old_locus_tag="B9G35_30005"
     CDS             complement(6888..7418)
                     /locus_tag="B9G35_RS30000"
                     /old_locus_tag="B9G35_30005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7425..8156)
                     /locus_tag="B9G35_RS30005"
                     /old_locus_tag="B9G35_30010"
     CDS             complement(7425..8156)
                     /locus_tag="B9G35_RS30005"
                     /old_locus_tag="B9G35_30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(8156..10120)
                     /locus_tag="B9G35_RS30010"
                     /old_locus_tag="B9G35_30015"
     CDS             complement(8156..10120)
                     /locus_tag="B9G35_RS30010"
                     /old_locus_tag="B9G35_30015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(11601..11750)
                     /locus_tag="B9G35_RS30020"
                     /old_locus_tag="B9G35_30025"
     CDS             complement(11601..11750)
                     /locus_tag="B9G35_RS30020"
                     /old_locus_tag="B9G35_30025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     ARG             complement(2..225)
                     /name="aadA15"
                     /gene="aadA15"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.14"
                     /coverage="28.52"
     ARG             complement(308..912)
                     /name="AAC(6')-Ib10"
                     /gene="AAC(6')-Ib10"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="98.97"
                     /coverage="99.01"
     Typing          10920..11498
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7429..8156)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.33e-150"
ORIGIN
        1 gactatttgc aacagtgccg tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc
       61 atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc
      121 atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat
      181 gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcatgat
      241 gtttaacgtt tgacatgagg ggcggccaag ggcgccagcc cttggacgtc cccctcgatg
      301 gaagggttag gcaacactgc gtgttcgctc gaatgcctgg cgtgtttgaa ccatgtacac
      361 ggctggacca tctggggtgg ttacggtacc ttgcctctca aaccccgctt tctcgtagca
      421 tcggatcgct cgcaagttgc tcggcgacgg gtccgtttgg atcttggtga cctcgggatc
      481 attgaacagc aactcaacca gagctcgaac cagcttggtt cccaagcctt tgcccagttg
      541 tgatgcattc gccagtaact ggtctattcc gcgtactcct ggatcggttt cttcttccca
      601 ccatccgtcc ccgcttccaa gagcaacgta cgactgggca tacccaatcg gctctccatt
      661 cagcattgca atgtatggag tgacggactc ttgcgctaaa acgcttggca agtactgttc
      721 ctgtacgtca gcaagtgtcg ggcgtgcttc ttctccgccc caccactcga cgatatgaga
      781 tcgatttagc cactcataga gcatcgcaag gtcatgctca gtcatgaggc gcagtgtgac
      841 ggaatcgttg ctgttggtca cgatgctgta ctttgtgatg cctaactttg tttggaaatg
      901 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
      961 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     1021 ttttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     1081 taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttttataaac cgtggagcgg
     1141 gcaatactga gctgatgagc aatttccgtt gcaccagtgc ccttctgatg aagcgtcagc
     1201 acgacgttcc tgtccacggt acgcctgcgg ccaaatttga ttcctttcag ctttgcttcc
     1261 tgtcggccct cattcgtgcg ctctaggatc ctccggcgtt cagcctgtgc cacagccgac
     1321 aggatggtga ccaccatttg ccccatatca ccgtcggtac tgatcccgtc gtcaataaac
     1381 cgaaccgcta caccctgagc atcaaactct tttatcagtt ggatcatgtc ggcggtgtcg
     1441 cggccaagac ggtcgagctt cttcaccaga atgacatcac cttcctccac cttcatcctc
     1501 agcaaatcca gcccttcccg atctgttgaa ctgccggatg ccttgtcggt aaagatgcgg
     1561 ttagctttta cccctgcatc tttgagcgct ctgatctgaa tatcgaggga ctgctggctg
     1621 gttgagaccc gcgcataacc aaaaattcgc ataaaaatgt accttaaatc gaatatcaga
     1681 cacgatgtgt ctattatgcc aaaatgacga tttaatggac actcaaacga agccgtttta
     1741 ctatgtctga taatttataa tatttcgaac ggttgcagtt gtgttaaaaa agccgtcagg
     1801 cagggaggcc gatatgcccg ttgatttttt gaccactgag caggttgaga gttatggcag
     1861 gttcactggc gaacccgatg aacttcagct ggcgcgttat tttcatcttg atgaagcgga
     1921 taaagaattt atcgggaaaa gccggggtga tcacaatcga cttggtattg ccctgcaaat
     1981 cgggtgtgtg cgttttctgg gcacttttct tactgacatg aatcatattc cttccggcgt
     2041 ccggcatttt accgccagac agctcgggat tcgtgatatc accgttcttg cagaatacgg
     2101 tcagagggaa aatacccgcc gtgagcatgc agcgctgata cgtcagcact atcagtatcg
     2161 tgaatttgcc tggccctgga catttcgcct tacccgtctt ttatataccc ggagctggat
     2221 aagcaacgaa cgtcctggcc tgcttttcga cctggcgaca gggtggctta tgcaacatcg
     2281 tattattctc cccggagcca ccacgctgac ccggttgatt tcagaggtaa gggaaaaggc
     2341 gacgttgcgc ctgtggaaca aactggcact gataccgtca gccgaacagc gttcacagct
     2401 ggagatgctg ctggggccaa ctgattgcag ccgcctgtct ttactggaat cactgaaaaa
     2461 aggccctgtg accatcagtg gtccggcgtt taatgaagca attgaacgct ggaaaactct
     2521 gaacgatttt ggcctgcatg ctgaaaacct gagtacactc ccggctgtgc gcctgaaaaa
     2581 tctcgcacgt tatgctggta tgacttcggt gttcaatatt gccaggatgt caccgcagaa
     2641 aaggatggcg gttctggttg cctttgtcct tgcatgggaa acgctggcgc tggatgatgc
     2701 actggacgtt ctggacgcca tgctggccgt tatcatccgt gacgccagaa agattgggca
     2761 gaaaaaacgg ctccgctcgc tgaaggatct ggataaatct gcattggcgc tcgccagcgc
     2821 atgttcgtac ttgctgaaag aagaaacacc ggacgaatcg attcgtgctg aggtgttcag
     2881 ctacatccct aggcaaaagc tggctgaaat catcacgctt gtccgtgaaa ttgcccggcc
     2941 ctcagacgat aattttcatg acgaaatggt ggagcagtac gggcgcgttc gtcgtttcct
     3001 gccccatctg ctgaataccg ttaaattttc atccgcacct gccggggtta ccactctgaa
     3061 tgcctgtgac tacctcagcc gggagttcag ctcacggcgg cagttttttg acgacgcacc
     3121 aacggaaatc atcagtcagt catggaaacg gctggtgatt aacaaggaaa aacatatcac
     3181 ccgaagggga tacacgctct gctttctcag taaactgcag gatagtctga gacggaggga
     3241 tgtctacgtt accggcagta accggtgggg agatcctcgt gcaagattac tacagggtgc
     3301 tgactggcag gcaaatcgga ttaaggttta tcgttctttg gggcacccga cagacccgca
     3361 ggaagcaata aaatctctgg gccatcagct tgatagtcgt tacagacagg ttgctgcacg
     3421 tcttggcgaa aatgaggctg tcgaactcga tgtttctggc ccgaagcccc ggttgacaat
     3481 ttctcccctc gccagtcttg atgagccgga cagtctgaaa cgactgagca aaatgatcag
     3541 tgatctgctc cctccggtgg atttaacgga gttgctgctc gaaattaacg cccataccgg
     3601 atttgctgat gagtttttcc atgccagtga agccagtgcc agagttgatg atctgcccgt
     3661 cagcatcagc gccgtgctga tggctgaagc ctgcaatatc ggtctggaac cactgatcag
     3721 atcaaatgtt cctgcactga cccgacaccg gctgaactgg acaaaagcga actatctgcg
     3781 ggctgaaact atcaccagcg ctaatgccag actggttgat tttcaggcaa cgctgccact
     3841 ggcacagata tggggtggag gagaagtggc atctgcagat ggaatgcgct ttgttacgcc
     3901 agtcagaaca atcaatgccg gaccgaaccg caaatacttt ggtaataaca gagggatcac
     3961 ctggtacaac tttgtgtccg atcagtattc cggctttcat ggcatcgtta taccggggac
     4021 gctgagggac tctatctttg tgctggaagg ccttctggaa caggagaccg ggctgaatcc
     4081 aaccgaaatt atgaccgata cggcaggtgc cagcgatctt gtctttggcc ttttctggct
     4141 gctgggatac cagttttctc cacgcctggc tgatgccggt gcttcggttt tctggcgaat
     4201 ggaccatgat gccgactatg gcgtgctgaa tgatattgcc agagggcaat cagatccccg
     4261 aaaaatagtc cttcagtggg acgaaatgat ccggaccgca ggctccctga agctgggcaa
     4321 agtacaggcc tcagtgctgg tccgttcatt gctgaaaagt gaacgtccct ccggactgac
     4381 tcaggcaatc attgaagtgg ggcgcatcaa caaaacgctg tatctgctta attatattga
     4441 tgatgaagat taccgccggc gcattctgac ccagcttaat cggggagaaa gccgtcatgc
     4501 agttgccaga gccatctgtc acggtcaaaa aggtgagata agaaaacgat ataccgacgg
     4561 tcaggaagat cagttgggag ctctggggct ggtcactaac gccgtcgtgt tatggaacac
     4621 tatttatatg caggcagctc tggatcatct ccgggcgcag ggtgaaacac tgaatgatga
     4681 agatatcgca cgcctctccc cgctttgcca cggacatatc aatatgctcg gccattattc
     4741 cttcacgctg gcagaactgg tgaccaaagg tcatctgaga ccattaaaag aggcgtcaga
     4801 ggcagaaaac gttgcttaac gtgagttttc gttccactga gcgtcagacc cctataactc
     4861 aatgagaaaa aagaattaaa ggtaaggaga cgctcatgag acgttccggc agcaaactgt
     4921 gtcttatcgc catgataaca gcgacagcaa ctctcactgc ctgtgctcca aagggcagcg
     4981 tggaacagca tacccggcat tacgtatatg cttcagatga cggttttgat cccaattttt
     5041 ccacccagaa agctgacaca acacgaatga tggtgccttt tttccggcag ttctgggata
     5101 tgggcgcaaa ggataaagcg accggaaaat cacggagtga tgtgcagcaa cgcattcagc
     5161 agtttcacag tcaggaattt ttaaactcac tccaaggcac aacacaattt gcgggtaccg
     5221 attaccgcag caaagacctc acaccgaaaa aatcaaggct actggctgac accatttcag
     5281 ctgtttatct cgatggctat gaaggcagac agtaagtgga tttaccataa cgcgtcatta
     5341 cgcgcaccgc tcagacgggc tcagggcgtt tccggcggct acgggataaa acaggcaaca
     5401 acccccccgg aaaactgccg gaaacgcgcc cgataaattt tatcctaatg catgcctatg
     5461 cacgcaactg catggcatat gttttgacgt aaaatggatg ccgtaagccc ttttttaccg
     5521 actttctgct tcatcagcct gactgacacg accatattct ttcagaccgc cccctgctaa
     5581 acttccttca tattgccgca ttaacccggg aggctccgca atgagcaaca ctacacacta
     5641 cgaaaacgcc aactttcttc gggaactggc tgaaagcctg ccccgcatac tgccggaagg
     5701 tggcccggac aaagctgcgc tgttgcagcg actggctaac gaagaactgg cccaggcaga
     5761 gtatgaagat caggttcgcg ccaaagtgac cgccgcccgt gctgacacgc gtccgggtat
     5821 gaccacggaa caactccgcc agcgcctgca tggccgttat caggaactgc gcgatgccgt
     5881 atgacgtgga atggaagcag ggagccatcg aggatgtaac gacgctgttt gactatatcg
     5941 cggaaaattc atccctgtgg gatgcgcaga acgttaccga ccgggttctc gctgcggcgg
     6001 acaaactggc cgacttcccg cgactctatg aagtggactc acgctatggc gaggacgttc
     6061 gccggatcag tctgatggga cagcatgtcc tctatgatgt tgatgaccag accagaaaag
     6121 tccgggttct ggccgtggtc ggccagcgcc agaatcccca tattgttctc taataaccgg
     6181 tgacggggac gcaattcatt gcgtggccgt cgccggttgg acaagcacag cgcgtcagcg
     6241 ttttccccac ggccaccacc ggcgcggtgt tccggccggg gtctctgctg tctccggttt
     6301 gtgttccagc agtctcatgg actgcatttc ctgccgtagc ccggccacct cctccctgag
     6361 tgcttttatc tccgcgatca acgcgtcgga ggccattttt tcagtctcac ccgacagtct
     6421 caactcgtcc cgctcgtgag tctcatcctg tcttaactca ccgtacaccc ggataagctc
     6481 gctggtatcc accacccggc cgccatcagc ttccgtacgg tatgatacgc ggcctttcgc
     6541 catatcccga tacagggaac ttcgggcttt acccgtcagt tccgtcgcct gtctcaccgt
     6601 cacccacgcc attgtctcac cccgctattt ttgagacagt atacctgaca ggcgcacacc
     6661 atgcccggaa ggaaagaatc atgacaaaac aggaaaaaac agccctcaat atggctaaat
     6721 ttattaagga gcagtcgctg ctattgctcg ataaactgaa tgagctggat atggataccc
     6781 cggccgacat gtgcgagcgg ctacacgagg atgcggaaac actctggcag gtgatgcggg
     6841 ataagctcgg ggaggaataa cagcaccggc atggccggtg ctggcattta ttttttgccg
     6901 aacggccacc accggcgggc aggtttgtcc tccggcagtt caggtaccgg cacgttaaaa
     6961 tagcggctgt tatattcccg catcagctcc gctttcagct tctcgttcac cgcgctttcc
     7021 ccgtgccgct cactcaccac gtcaatcatc gcgttaatct gctccggtga gcacttcccg
     7081 gcatcaacca cctgcacacc gttctcgatg gcgaaccgct ccaccttgcg atagaggtaa
     7141 tcacacagct tcattcctgt gggtttgttc cgttcccatt gcggattttt accagtgaaa
     7201 aagtacgtgg ctttttcgtc gctgttgagc cgctcgtcat acatcccgat ggatttcatc
     7261 atcgccagat gctgggcccg gtcgacaaaa cagaaaacat acgccccggt ctgccaggcg
     7321 agcaccacca tatcttccag atgctcgctg gtcggcatga tgcagcgctc gccgttccgg
     7381 tcctccgcgt cttcaataaa taccatgtac agcgccataa tccctcactc cgttgtcata
     7441 aatttaaagc acgcaaacaa ccgttcacga tccagctctg ccgttttgcg ggcctcgccg
     7501 gtctgtgggt tcacgatttc atccaccgac aggattgccc gctgcaccct gacctgcgtc
     7561 gcccggtccg ggcagatgta ccagatttca tcgatgccgt tagctttgcg gttgaacaga
     7621 tacccggaca ccacttcctg ataccggcga cggctcttga cggtgcgctc cacctcaaac
     7681 gccaccgttt taccgtccag ctccgccagg gcgtccggca ctttcatgcc tttaagactc
     7741 agacggtgca gaatgcgcca gccggtggcc cctctggctt ccatatcaag gcgcagggat
     7801 tgcacggcca gccggtgcgg cagatgtgct gccgacaccc ttgacggctg aaacggaata
     7861 acatccgcca gcggttcgtc ctcgtcgaac gagaacgcca cgccatgcgg cgtcagcccc
     7921 cacacgctga cctgacggcc gtccaccgga tatttggtcc ggaccagcag ccctttcgcc
     7981 accaggctgt cgaggaagcg gtagaccggc tgcaccgccg caatccccag ccgccgcgca
     8041 accagctccg ccgtggaaca cagctcttcc cgcaaccagc gcagggtcag ccgggttttc
     8101 tccgcgttcc gcgcctgtcg tttcgcgtgg ctgacgttgt acagctccag cgccatcagt
     8161 aaatccccgc aaaatcatca tccgctttcg gcccgccgcc gtcggccggg ggccggaact
     8221 ccgccagggg atcggttttc gccagcaccg ggaaacgctg caacgggatt tcccggcgct
     8281 caacccgcag cggactgctg aacgccatcc gggccacacc cagcccggtc atgaccgcga
     8341 acccgtccgg caggtgttgc agggtgttca cgtcaaacag cggccgcgcc tccttctgga
     8401 tgaagctgtc cccgctgata tgctccacgt tccccgcctc ccggctggca cggcggcgct
     8461 ccacgtccac cctgatttcc ccggtctgcc ccgccgccca ctgcgcggac tccaggctgg
     8521 ccgcccggta aaaccagcgg atcggcgtgt tatccagtac cgtggttttc acaaattcag
     8581 cgggcagatc ctgcccgcac tgcccgaaat cgcccagcga ctggtgcgcg agcaacagat
     8641 tgcagtcccg gctgcgcagc gtccccagtg cgttcagtac atacttcgac agcagatatt
     8701 taatttcatc gagcatcacg ctgacgtgtg gccactcccg gaactcgtcc cgcgcaatga
     8761 tgatttgcgc gcagcgggca aacagcattt tttgcacacg gatcaccgcc tcgtcgtcca
     8821 tcgagccgat aacgtacagg cagccgccgc cgttgataat ccccgccaca tcgatgccac
     8881 tgtccgtctg caggacgctc aggtcagcga cgttttccag cctagtgatc aaccccttca
     8941 cgtccttttt cagggcctcc ggcagcgcgt acgccgccgc cagcacctgc tgaatgtttg
     9001 ccccctgcgg aaactgctcc gcaatcagct tcgccgcttt ctgctcgctg atgcggtagt
     9061 ggtccgccac gtcgccggtc tcgcccaggt taagcccggc aatcagcagg tttttcagcg
     9121 cgtactggtc gagatcgcgg aacagattca gctgcgccgg tttccccggc cgcagatcca
     9181 gcagggtgaa gtccggacag tgcgcccgga agacgtgcgg cgcccatgcg tcccctttgg
     9241 ggtcgaagta caccaccgcg tccccgaaat cgcgtacgca gcgaatgagc gcgttggtgg
     9301 ccatcacgct cttgccgctc ccgggcaggc cgaggatctg aatgttggtc ttgcgccagg
     9361 tatcacgcgg aaccagcacc ggcttgccct gctcatcctt acccagaaac accgcctgct
     9421 cctgccgggc ctgctccgag gaaaaatcgg ccagcgcatt aaccggtacc ggctccccga
     9481 aatgcaggtg gcgcacgtcg gtcatctctc ctgtccgctg gctgctgcgc cggctctcct
     9541 gattcagttt cgcgttcacc gcctcgtcag cagccgcaaa atgaccgtca agcagcagga
     9601 acgccagcag ccgcgccaac acaatcccga cagccagccc gcccgcgcac caggcaatcc
     9661 cgaacaggtt agccggctgc agcaggtaat aatctgcgtt tgcgtagagg tccggacgca
     9721 ggacggtttc tccccagctc tcgataaaca cggcgacaac cagccccccc agaagggcca
     9781 gaccggccgc gccggtcagc cagtacgcgt agttccagac atgtcgacgt gccctgacca
     9841 tccgcccggg cgaatccagc gaggccagca ccgaaaatgg cgctggccgt ttattatcgc
     9901 gcttatagcg atcggcatac tcccgtcgct ccttatgact cagccccaca aaacgcatcc
     9961 gtccggccca aagttcagcc agaccgaaaa acaggccgat aactaggaaa gccccggcca
    10021 gaccgtaata ctcctggtac agccagctga cccacggata aatctgccgg gccagctccg
    10081 gaatgtgcca cttccaggca agcgaatccg tattaaacat gttctctctc cttcagacga
    10141 tggtttgccg gcgtgatgtg cagcccccgc tgcacaagcc gcggacatgc cccaaggcat
    10201 ggcggcggcg gtcacgtccg tggtgattgc gttcagcttt caccacggag gccagcctct
    10261 ttccgcccca tgcggaaaag gcgcagcccc ggaggaacaa cgagcacgcc ccagcgtgtg
    10321 agaggaaaag gcggcacccc agtgcggtct tttccggtgt tccggccccc gtcagagttc
    10381 gccatagcga gcggctgacc acccgcaaaa tcaggagcag ctttgctgct tcgccaggtg
    10441 cgtccagcac cgtggatttt tgtgggtggt caggcgtgag cctgacgggg gccgggtgaa
    10501 tttctggcgt ggccggcgtc cagccggacg gagccagaaa gagggggaga gaccgtgcgt
    10561 cggtggcgtc cagccagcgt aagcagggga tatccccctg aggtcagcca cgccctcgcg
    10621 gggatgacct gtatttctcc gtaaggagaa aaaagcaaca gagataggtt aacgtaaaat
    10681 gttcagcgaa aaaccgatat gattcaccta tcctggtaat ttattatcag ggaatataaa
    10741 gacttaatca ggggacaata tgccacatca gcgcgtccgg tgtccgtctt gtaggtgccg
    10801 gggaaattgc gcctcctcgc tcactcggtc gctacgctcc ggccgtgaga ctgcggcggg
    10861 cgctgcggac acatgcaaag ttacccacag attccgtgaa taagcagggg actaacatgt
    10921 gaggcaaaac ggcaaggcca cgcaggtggc gtttttccat aggctccgcc ctcctgactg
    10981 agttcacata aacagacgct ttttcggtgc atctgtggga gccggtggct caaccatgaa
    11041 tcagtcagga gtggcgaaac ccgacaggac ttaaagatca tcaccgtttc cgatgaatcg
    11101 ctccctcttg cgctctcctg ttccaaccct gccgtttccc ggatacctgt tccgcctttc
    11161 tcccttgcgg gaagtgtggc gctttctcat agctcacact ctggtatctc ggctcggtgt
    11221 aggtcgttcg ctccaagctg ggctgtaagc aagaaccccc cgttcagccc gactgctgcg
    11281 ccttatccgg taactgttca cttgagtcca acccggaaaa gcacgcaaaa cgccactggc
    11341 agcagccatt ggtaactggg agttcgcaga ggatttgttt agctaaacac gcggttgctc
    11401 ttgaagtgtg cgccaaagtc cggctacact ggaaggacag atttggttgc tgtgctctgc
    11461 gaaagccagt taccacggtt aagcagttcc ccaactgact taaccttcga tcaaaccacc
    11521 tccccaggtg gttttttcgt ttacagggca aaagattacg cgcagaaaaa aaggatctca
    11581 agaagatcct ttgatctttt ctactgaacc gcgatccccg tcagttcaga ggaggacgat
    11641 ggtgccaccg ttccaccctg aacatcccgg atatagtttg cctgacatgc gaccagaaga
    11701 aagcctgaaa aaatcaatat aaaaagaaaa acaaccttta cctttttcat ataccttccc
    11761 tgtatttaaa aaccccggta ttcatccctg aacaccggaa gtctgtattt caaatcatcc
    11821 ttgataactt ttgcttacca gttaccatcc cggtaaacaa aggatacaaa gtaatcaaat
    11881 ttactaatat cgattacatt tttaaagtgt ggctgtaata tcgaaaccca tgatttttca
    11941 acatcaaacc aaccattatt aatattatcc tttaaaggca tccc
//
