LOCUS       NZ_CP019907            20225 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain MDR_56 plasmid unnamed2, complete sequence.
ACCESSION   NZ_CP019907
VERSION     NZ_CP019907.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06344815
            Assembly: GCF_002009315.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20225)
  AUTHORS   Lawsin,A., Zhu,W., Batra,D. and Laufer-Halpin,A.
  TITLE     Complete genome sequence of Escherichia coli containing MCR-1 gene
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20225)
  AUTHORS   Lawsin,A., Zhu,W., Batra,D. and Laufer-Halpin,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2017) Clinical and Environmental Microbiology
            Branch, Centers for Disease Control and Prevention, 1600 Clifton
            Road NE, Atlanta, GA 30333, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: PacBio Hierarchical Genome Assembly Process v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 90.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002009315.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:30:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,369
            Genes (coding)                    :: 5,046
            CDSs (with protein)               :: 5,046
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 323
            CDSs (without protein)            :: 323
            Pseudo Genes (ambiguous residues) :: 1 of 323
            Pseudo Genes (frameshifted)       :: 112 of 323
            Pseudo Genes (incomplete)         :: 220 of 323
            Pseudo Genes (internal stop)      :: 61 of 323
            Pseudo Genes (multiple problems)  :: 59 of 323
            Pseudo Genes (short protein)      :: 1 of 323
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019907.1.
            Bacteria and source DNA available from CDC-DHQP-MDR-56.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MDR_56"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA:NY"
                     /collection_date="2015-05"
     gene            complement(join(20208..20225,1..2226))
                     /locus_tag="B1200_RS27805"
                     /old_locus_tag="B1200_28550"
     CDS             complement(join(20208..20225,1..2226))
                     /locus_tag="B1200_RS27805"
                     /old_locus_tag="B1200_28550"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="WP_249533336.1"
                     /translation="MVALLKPKTFRDEPSVNEKYLQHSQHLDDYIISMRNGDLLAFFKV
                     DGRTHDCASDRELITWNDDLNTLIKSFGTDHVEVWSHEYHHQSKEYPDSEFTQFFPRYL
                     DEYNRRLQTGSNQMVNDLYLTVLYRQIGDKTQKFLAKFERPSADELAQMQAEAFTGMEE
                     VCEQIMEGMKGYGIKRLGIYYRDKNGEEIALTDAEEKQSLLEVDEADIFDEPVMLNVAD
                     TVPPTAHAYSKALEFLYFLVNLEWSIVPVCRDRIGSYIMENRVVSSVWGDIVQVRTLDS
                     NFYTTAIEIRDYEKNTEPGQFNLLKEADFEYLLTQTFSCISEASAKTLLERQEKSMMET
                     GDRSQSQLEQLNTALDMLLSKEIVMGYHHATIHVFDTDQRAVQRKARKAKVMLSQCGFV
                     GGGVGLASEAAYFAKIPGNQKWAPRPTPINSWNFFHFSPYHNFMRGKPDNNPWGPAVTM
                     FRTANGTPLYFNFHVTPLEERSIGKRPLGHMLLTGTSGEGKTTLLDFLLCQCMKFNPRM
                     FCYDRDRGMEPFIRSVGGYYKVLQQGVKTNFSPLKQKGTPRNIATTKNIMRVCAEITNN
                     GPLDKDMGMELNKAVDAVMGEHSLVPPEARDITRLNGYINHPYLKDLIKDWTNEGQYGW
                     LFDNDEDALDLDAHDVFGFDLTEFIAAEGEIPSPARTPLLMYLLYRIRNSIDGKRRVIQ
                     CFDEFHAYLDDPVLKLEIKRGIKTDRKKDAIYLFSTQEPNDALASSIGNTCNR"
                     /name="conjugal transfer protein TraB"
     gene            complement(2227..2544)
                     /locus_tag="B1200_RS27810"
                     /old_locus_tag="B1200_28555"
     CDS             complement(2227..2544)
                     /locus_tag="B1200_RS27810"
                     /old_locus_tag="B1200_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000496058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="WP_000462629.1"
                     /translation="MFENGQRPLFKGATRVPRLAGVPRTVALVIFMISATLFMTLHLWS
                     LLVFAVLWSIAAALTKYDDRMFRILSLWLQTKFRNAHDTPFKQWSGSSYSPVDYKKKEL
                     K"
                     /name="VirB3 family type IV secretion system protein"
     gene            complement(2584..2880)
                     /locus_tag="B1200_RS27815"
                     /old_locus_tag="B1200_28560"
     CDS             complement(2584..2880)
                     /locus_tag="B1200_RS27815"
                     /old_locus_tag="B1200_28560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_015060075.1"
                     /translation="MVTFFKNNGFALLVALLCIAAPELALAAGGADTGASTGNNIKSWL
                     DTWIPIACTIGVIVSFLVWLFHLISAGFMFRLAIAMVGIGSASYLVSLTGIGS"
                     /name="TrbC/VirB2 family protein"
     gene            complement(3127..3909)
                     /locus_tag="B1200_RS27825"
                     /old_locus_tag="B1200_28570"
     CDS             complement(3127..3909)
                     /locus_tag="B1200_RS27825"
                     /old_locus_tag="B1200_28570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_078081123.1"
                     /translation="MKKSWCLIAVCISGLSFQGVAHAASASDTIASLAARCAPDVSPLT
                     MAYIVGHESGNNPYFINVNRGANLKRQPQTEAEAVEVANRLIKNLKKDESIDMGLAMIN
                     SNNLSSLGITVEQVFKPCINLRASQTILKDCYSTALKSNPPGQVALRHALSCYNTGSQV
                     RGISNGYVTKVINVAKQSDLKIPTLLPDGQNEEAESQPVSAKGATFEGEHDAFTGESDS
                     GGDVFSQDNTDAFLTKAEQKGSEVMNDKNLSPVAVSNN"
                     /name="lytic transglycosylase domain-containing protein"
     gene            4009..4326
                     /locus_tag="B1200_RS27830"
                     /old_locus_tag="B1200_28575"
     CDS             4009..4326
                     /locus_tag="B1200_RS27830"
                     /old_locus_tag="B1200_28575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="WP_078081124.1"
                     /translation="MLVPLQSEKRPKGTPVYRDPENPFNTWSGYGKRPVWLQERLSDGY
                     LLDEMKLPGAVVDRDTKSTRYRDPANPDNTWTGVGRRPKWLVELLENGVDLDDLKIRER
                     E"
                     /name="H-NS histone family protein"
     gene            4330..4680
                     /locus_tag="B1200_RS27835"
                     /old_locus_tag="B1200_28580"
     CDS             4330..4680
                     /locus_tag="B1200_RS27835"
                     /old_locus_tag="B1200_28580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032416642.1"
                     /translation="MGNVIEFKRPKGERNQKDKMPKRGILLWFKKTALSLKNNLFTYSK
                     SCCCICLFFVSAASRKLLKLIATLSIVLFLVEWFMGRLGAPEFYNLLFLMGLLLVINVI
                     STSCIKWIRGNV"
                     /name="hypothetical protein"
     gene            4677..5015
                     /locus_tag="B1200_RS27840"
                     /old_locus_tag="B1200_28585"
     CDS             4677..5015
                     /locus_tag="B1200_RS27840"
                     /old_locus_tag="B1200_28585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223226685.1"
                     /translation="MSKLRTVAKILLMYCIFSSASAFAEGNTKGDPCLAFTCMAGKLQG
                     NEATSGPDCVMGRQSFFEIRIYTPYYNPGATAAARKVFLSSCPMTTISENKNTLELIIQ
                     RYGRSFQE"
                     /name="hypothetical protein"
     gene            5019..5327
                     /locus_tag="B1200_RS27845"
                     /old_locus_tag="B1200_28590"
     CDS             5019..5327
                     /locus_tag="B1200_RS27845"
                     /old_locus_tag="B1200_28590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032416643.1"
                     /translation="MKKLIPAIIAAALLSGCATQNDAVALSNRISDQQKQINALSVRLQ
                     SAEHRLSQQESAFRSEQTQNSSYCYLNGVKYSQGSVFAGRICDGSSGSAAWRLYSHR"
                     /name="hypothetical protein"
     gene            5612..5797
                     /locus_tag="B1200_RS27850"
                     /old_locus_tag="B1200_28595"
     CDS             5612..5797
                     /locus_tag="B1200_RS27850"
                     /old_locus_tag="B1200_28595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006118530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="WP_024562121.1"
                     /translation="MAEPYLKNRRRFTSSLDNQLVPLFDALAAKTRIPKSRLLDEAIED
                     LLVKHGVPGKKVDSQN"
                     /name="ribbon-helix-helix domain-containing protein"
     gene            complement(5794..6402)
                     /locus_tag="B1200_RS27855"
                     /old_locus_tag="B1200_28600"
     CDS             complement(5794..6402)
                     /locus_tag="B1200_RS27855"
                     /old_locus_tag="B1200_28600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029810.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_032449362.1"
                     /translation="MYIFGYLRASAHDQDASRAQQRLKAFALEHNHRVAGWYVENASGA
                     SLQRPELMRLLNDAEKGDLILIEQVDRLSRLDDEGWQQLKRMMQEKNLAIISLDLPTSY
                     AALMPHGSDEFTRSMLTAINSMMLDMLAAIARKDYTDRRRRQQEGVRKAKEKGVYRGRM
                     PDQNKHDLIRSLRHQGKSISETARLAGVSERTVTRVSKS"
                     /name="recombinase family protein"
     gene            complement(7047..7286)
                     /locus_tag="B1200_RS27865"
                     /old_locus_tag="B1200_28610"
     CDS             complement(7047..7286)
                     /locus_tag="B1200_RS27865"
                     /old_locus_tag="B1200_28610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_078081126.1"
                     /translation="MTERQQNRNVCAVGKAREGMTIDVSVNPFQSTGTVWLYDYSRNTV
                     IEKRSDGTNITHISPQGGKRFFLPVEPVKPKSDT"
                     /name="hypothetical protein"
     gene            7426..8307
                     /locus_tag="B1200_RS27870"
                     /old_locus_tag="B1200_28615"
     CDS             7426..8307
                     /locus_tag="B1200_RS27870"
                     /old_locus_tag="B1200_28615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020323144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="WP_078081127.1"
                     /translation="MAEDSKQLTKRQQKAIDTAALIRQEPPQGDDMAFTHSILCQVGLP
                     RSKVEGREFMRRSGDAWLVVQAGWLDEGNGPVEQPLPYGAMPRLTFAWISSYALRNKTR
                     EISIGHSASEFLRLMGMELQGARHRTLRTQMQALAACRLQLGFKGRTYNGQPVEQFDAW
                     LKDGDTKQLTLWPGTLTLSEGYYNGLIESAVPLDNRALHVLKGSALALDIYAWLAHRLH
                     RIEGRPVMLYWMKLREQFAQEYSGKNADKDFKRAFMPALKQVLSVYPAAKVDQVKGGLL
                     LYSSPPPIPYKS"
                     /name="replication protein RepA"
     gene            8881..9219
                     /locus_tag="B1200_RS27875"
                     /old_locus_tag="B1200_28620"
     CDS             8881..9219
                     /locus_tag="B1200_RS27875"
                     /old_locus_tag="B1200_28620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006118533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrfB-related DNA-binding protein"
                     /protein_id="WP_024562321.1"
                     /translation="MARKQTRSMTQEEWDRLLPAMQTFTHLSTEIGHSVLVKGESNKDV
                     AERVGRTKQNVGSTVKRIWDLYQSLAVDIEGEKLRKVDVWIPEKLALKVLKEAEKYAIN
                     QSKVEQSE"
                     /name="TrfB-related DNA-binding protein"
     gene            9327..9857
                     /gene="ssb"
                     /locus_tag="B1200_RS27880"
                     /old_locus_tag="B1200_28625"
     CDS             9327..9857
                     /gene="ssb"
                     /locus_tag="B1200_RS27880"
                     /old_locus_tag="B1200_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020323149.1"
                     /GO_function="GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA];
                     GO:0006281 - DNA repair [Evidence IEA]; GO:0006260 - DNA
                     replication [Evidence IEA]; GO:0006351 - DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="WP_078081128.1"
                     /translation="MASRGVNKVILVGNLGQDPEMRYMPNGGAVANLRLATSESWRDKA
                     TGEQKEVTEWHTVVIFGKLAEVAGEYLRKGSQVYIEGQLRTRKWQDKDQQDRYSTEVVV
                     SMNGVMQMLGGRQGGGAPAGQGQPQQQFSGGGAPAAAPGPQQPQQGAMPDFDDDIPFAP
                     VTLPFPNHAIHAL"
                     /name="ssb"
     gene            complement(9887..10675)
                     /locus_tag="B1200_RS27885"
                     /old_locus_tag="B1200_28630"
     CDS             complement(9887..10675)
                     /locus_tag="B1200_RS27885"
                     /old_locus_tag="B1200_28630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174710.1"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]; GO:0004222 - metalloendopeptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="WP_078081129.1"
                     /translation="MTLPTPETYEELQHAYDFFDERLFNNELPPCLITLQREKRTYGYC
                     SFQRFAGRESGQMVDEIAMNPVYFSIRTIKATLSTLVHEMVHQWQFHFGKAGRRGYHNK
                     EWAALMERVGLMPSDTGQPGGRKVGQSMTHYIITGGAFDRACDELLTRNFQLSWMDRFP
                     PYQPKPGAPQSPSGKGYIDDEDDDGEYPPDDDYTDEPGELDNEIIEAMKFVSPPPETPV
                     NKSNRAKYTCPVCHVNLWGKPGLVVFCGGEHCEKAELQPS"
                     /name="SprT family zinc-dependent metalloprotease"
     gene            10854..11276
                     /locus_tag="B1200_RS27890"
                     /old_locus_tag="B1200_28635"
     CDS             10854..11276
                     /locus_tag="B1200_RS27890"
                     /old_locus_tag="B1200_28635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="WP_024562320.1"
                     /translation="MANIVTAKIYGPAQKMRFLPYAFAEFYLQAENYVCSFMKKYAVGY
                     DGGCWEYITYENGACAFIAPDGYQLTLPNYFDEPVSAKEAGIIVALYAYSHFCCVAFER
                     GWQRVNETMAERYHTLRDFTETLPPESQSRIFRAID"
                     /name="antirestriction protein"
     gene            11321..11584
                     /locus_tag="B1200_RS27895"
                     /old_locus_tag="B1200_28640"
     CDS             11321..11584
                     /locus_tag="B1200_RS27895"
                     /old_locus_tag="B1200_28640"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227650696.1"
                     /translation="MLMVTTDEVWFRYLDYSGQTKAVRVASVRFWPDIQETIFPPLQVP
                     EGKRRVVRCRCGSNDWNEDGRWLGEYCCASCGQYIQVFEKKD"
                     /name="hypothetical protein"
     gene            11864..12139
                     /locus_tag="B1200_RS30885"
                     /old_locus_tag="B1200_28645"
     CDS             11864..12139
                     /locus_tag="B1200_RS30885"
                     /old_locus_tag="B1200_28645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020302556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdR"
                     /protein_id="WP_249533337.1"
                     /translation="MKQSGFFSRLRFAAEAAAYREAWPGHDGGVVLIWEGKAYGWKNCL
                     RDAGGERPGAIAIDTDGHVFIAEGGNEYDGAKCWVAMIDRENEKNG"
                     /name="antirestriction protein ArdR"
     gene            12123..12542
                     /locus_tag="B1200_RS27905"
                     /old_locus_tag="B1200_28650"
     CDS             12123..12542
                     /locus_tag="B1200_RS27905"
                     /old_locus_tag="B1200_28650"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162781990.1"
                     /translation="MKRMVSLFYAARGLWRVVANFIREAVRSSLLAVMHMVSVHIPVLI
                     FLAVFVLAFSGFGLLVIKVLAIVLHPVTQGPIVGSIANVLDALNQALSPMVYFKIAVPV
                     FFVVVLTSLFGSVRVNWKKIVTRWQRLVEVGKDKR"
                     /name="hypothetical protein"
     gene            complement(12592..12687)
                     /gene="dqlB"
                     /locus_tag="B1200_RS31125"
     CDS             complement(12592..12687)
                     /gene="dqlB"
                     /locus_tag="B1200_RS31125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_274611345.1"
                     /translation="MINVLIVVLRAVVAVSNAVIAVLELIRQFFD"
                     /name="dqlB"
     gene            13717..14508
                     /locus_tag="B1200_RS27915"
                     /old_locus_tag="B1200_28660"
     CDS             13717..14508
                     /locus_tag="B1200_RS27915"
                     /old_locus_tag="B1200_28660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020323375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD29 domain-containing protein"
                     /protein_id="WP_078081133.1"
                     /translation="MTTLTVGQCLTSFNNEYVVSAVNLADDKISYTILGLNAPTCAPLL
                     ETSLRFYQVIDKTLPLDELRARRQVVQSVTDQREARHQAKEDARQLENERASADPENAG
                     LLTTATESNTTKLAAKNIRILLKKHFPGVKFSVRMRDYNALYVSWTDGPTKEAVEAITD
                     KFEEGSVNSMEDMYEYNITGFHRVYGGVKYLFCSRDLTDALIAESIELLRKEYGETTIP
                     ADVTLEAYKSGALAGRGHDCFTWGLAAQIRINAGKVDKSSR"
                     /name="LPD29 domain-containing protein"
     gene            14677..14946
                     /locus_tag="B1200_RS27920"
                     /old_locus_tag="B1200_28665"
     CDS             14677..14946
                     /locus_tag="B1200_RS27920"
                     /old_locus_tag="B1200_28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020323374.1"
                     /translation="MNYLIIIPIAFILAAAALALPWFMVRQGYREQVQLGGACTTVLGL
                     AASTGVFSYADNMPLALLYGSASVVSFLYALDCFLPLYLSRKSH"
                     /name="hypothetical protein"
     gene            14989..15189
                     /locus_tag="B1200_RS27925"
                     /old_locus_tag="B1200_28670"
     CDS             14989..15189
                     /locus_tag="B1200_RS27925"
                     /old_locus_tag="B1200_28670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020323376.1"
                     /translation="MENGKLAQQQGFDLALQILKSQRGYYIGTATSEGYPVSRESAEYF
                     PDSSTAQQALTDNRWTQREDC"
                     /name="hypothetical protein"
     gene            15244..15741
                     /locus_tag="B1200_RS27930"
                     /old_locus_tag="B1200_28675"
     CDS             15244..15741
                     /locus_tag="B1200_RS27930"
                     /old_locus_tag="B1200_28675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020323377.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="WP_078081134.1"
                     /translation="MADHDDAPEKIKCLECGKEFSFLAPHLSKAHQMNARQYRERWGIP
                     LHTPLASAEHSRQCRENVLRRIRRGEIRPADQLALMAEGRKNAPERATSTRLHKVAAAN
                     VARVHQIWKHSPVVKVVSDTLRDEAVQRMTARKVTGEKVKDIAADLNLSVGCLYKWVAD
                     AK"
                     /name="MucR family transcriptional regulator"
     gene            16519..16725
                     /locus_tag="B1200_RS27935"
                     /old_locus_tag="B1200_28680"
     CDS             16519..16725
                     /locus_tag="B1200_RS27935"
                     /old_locus_tag="B1200_28680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020323152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="WP_020323152.1"
                     /translation="MKQHIAAIIREYNTPTVTVEVANTDRYDSEQIEIRHVVDGRLAWR
                     AWDYETGFENDLHRELAYYHIPA"
                     /name="DUF905 family protein"
     gene            16799..16981
                     /locus_tag="B1200_RS27940"
                     /old_locus_tag="B1200_28685"
     CDS             16799..16981
                     /locus_tag="B1200_RS27940"
                     /old_locus_tag="B1200_28685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000170167.1"
                     /translation="MTKVTTPSQLKAELESQKTYLLEACLMAFNQLPNQRTKGAFPSTY
                     ALAAKIDHLLQQEKK"
                     /name="hypothetical protein"
     gene            complement(17040..17390)
                     /locus_tag="B1200_RS30890"
     CDS             complement(17040..17390)
                     /locus_tag="B1200_RS30890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_249533334.1"
                     /translation="MDKTERERMLARRRQARRRERLRAEGVSVTVTLTHDEAAQLDELR
                     QVRRMGKQPYSPNEFFQLLLIRNWQQWEQEKAALGTCQHCGKSKAGGGCGGEFQKETYQ
                     CWLAQDANALNL"
                     /name="hypothetical protein"
     gene            complement(17454..17822)
                     /gene="stbC"
                     /locus_tag="B1200_RS27950"
                     /old_locus_tag="B1200_28695"
     CDS             complement(17454..17822)
                     /gene="stbC"
                     /locus_tag="B1200_RS27950"
                     /old_locus_tag="B1200_28695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein StbC"
                     /protein_id="WP_227650693.1"
                     /translation="MDKQPDKLDVLMDWFLGDAKEILEAMKLMKAEQADMLQQLGELKS
                     ALELTADDSRAEIIGSLRDIQAAMKEENKARSDFLARWQSLQHNNASTIVNRVVIMTAV
                     CSIVGAAIGTALTLLILK"
                     /name="stbC"
     gene            complement(17825..18541)
                     /gene="stbB"
                     /locus_tag="B1200_RS27955"
                     /old_locus_tag="B1200_28700"
     CDS             complement(17825..18541)
                     /gene="stbB"
                     /locus_tag="B1200_RS27955"
                     /old_locus_tag="B1200_28700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011279206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StbB family protein"
                     /protein_id="WP_020323147.1"
                     /translation="MKVAVINYSGSVGKTLVSTYLLAPRLKDAKFFAVETINQSASDLG
                     IENVTSFKGDDFSRLIEDVVFEDAAIIDIGASNVEPFLMAMSRFDGGANEFDMYFIPVT
                     PEDKAIKESMKTAHTLSKAGVDNKKIIFVPNRVNPGDDVENVFGPIFDFVKQTKVGRIN
                     KNSAIYNSEVYEYLAHHKISFETLTAEDAEEFKARAKASADADERRKMARRYTYMKQAI
                     PVKSNLDKTFSLLIGD"
                     /name="stbB"
     gene            complement(18550..18981)
                     /gene="stbA"
                     /locus_tag="B1200_RS27960"
                     /old_locus_tag="B1200_28705"
     CDS             complement(18550..18981)
                     /gene="stbA"
                     /locus_tag="B1200_RS27960"
                     /old_locus_tag="B1200_28705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020323173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein StbA"
                     /protein_id="WP_078081135.1"
                     /translation="MKPKSIRAAIQMMLPEVEEMLSLGVSREEVFKAVSERFGLENVNV
                     RSFDTALYRARQNRAKHATPDSVLHNTESAGASEQQKKPVLHNTEKPAKSEQVKKDVLH
                     NTQSATEGDETKQSPGIIGKEFFDEARAEFDPNEFIKKY"
                     /name="stbA"
     gene            19451..19858
                     /locus_tag="B1200_RS30895"
                     /old_locus_tag="B1200_28710"
     CDS             19451..19858
                     /locus_tag="B1200_RS30895"
                     /old_locus_tag="B1200_28710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traK protein"
                     /protein_id="WP_249533335.1"
                     /translation="MPIITCKVTDEEKELIDRLAGTNRSDYMRRCLLAGSGGRIGGLSQ
                     VTEQINEVNRRLDILFDQVSETDMSAFSSELKGLVKQLSQLKLPPPGQMMLHESLAIET
                     VILLRSMATPANMKAAWGELERNGYKVWESK"
                     /name="traK protein"
     Typing          7427..8307
                     /name="IncN"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.073"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..2226)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.922"
                     /coverage="87.0"
                     /evalue="0.0"
     Typing          complement(2231..2544)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3e-64"
ORIGIN
        1 accaatgctg cttgcgagcg cgtcatttgg ctcttgcgtg gaaaacaaat aaatcgcatc
       61 tttcttacgg tctgttttaa taccgcgctt tatttccagc tttaaaacag gatcgtcaag
      121 ataagcatga aattcatcga agcactgaat gacacggcgc tttccgtcaa ttgaattacg
      181 gattcggtaa agcagataca ttaacaatgg cgttctggcc gggctgggaa tctcaccttc
      241 agcagcaatg aactcggtca ggtcgaagcc gaatacatca tgagcatcga gatctaacgc
      301 atcttcatca ttatcaaaaa gccagccata ctggccttca tttgtccagt ctttgataag
      361 gtctttaagg tatgggtgat tgatgtatcc gttaagacga gtaatatcgc gagcttcagg
      421 aggaaccaat gaatgctcac ccatcacagc atctactgcc ttattgagtt ccatccccat
      481 atctttatct aacgggccgt tatttgtaat ttcagcacag acacgcataa tatttttagt
      541 ggttgcaata ttacgcggtg tgcctttctg tttgagcggt gagaagttag tttttacgcc
      601 ctgctgtaaa actttatagt agccgccgac actgcggata aacggctcca ttccccggtc
      661 acggtcatag cagaacatac gcggattaaa cttcatgcac tggcataaca ggaaatccag
      721 cagcgtagtt ttaccttcac cagatgtacc tgtaagcaac atatgcccca gcgggcgctt
      781 cccgattgag cgttcttcca gtggggtgac gtggaagtta aaatacagcg gtgtaccgtt
      841 cgcggtacgg aacatcgtca cagccggacc ccacggatta ttgtccggtt tgccgcgcat
      901 aaaattatgg tacggggaga aatggaagaa attccatgag ttgatcggcg ttgggcgtgg
      961 tgcccatttc tgattacccg gaatcttcgc aaagtaagcc gcttcagagg ccagccctac
     1021 gccgcccccc acgaagccac actgacttag catgacttta gctttacggg ccttgcgctg
     1081 cactgctcgc tgatcggtat cgaaaacgtg aatagtggca tggtgataac ccatcacaat
     1141 ttctttagaa agcagcatat ccagcgctgt attcaactgt tcaagctggg actggcttct
     1201 gtcgccagtt tccatcatgg acttctcctg gcgctccaga agcgttttag ctgaagcctc
     1261 ggatatgcaa gaaaacgttt gagtcagcag atattcaaag tcagcctctt tcagcaaatt
     1321 gaactggccc ggttccgtgt ttttttcgta gtcacggatt tcgatagctg tagtataaaa
     1381 attgctgtcc agcgttctga cctgaacaat atccccccat acagaactga caacacggtt
     1441 ttccatgatg taagaaccga tacggtcacg gcaaacagga acaatagacc attccagatt
     1501 gaccaggaaa taaagaaact ccagcgcttt agaataagcg tgagcagtcg gcgggacggt
     1561 atcagcgacg ttgagcatta ccggttcgtc gaaaatatct gcttcgtcca cttcaagcag
     1621 cgactgtttt tcttcagcgt cagttagcgc tatttcctca ccgtttttat cccggtaata
     1681 aatacccagg cgtttgatgc catagccctt catcccctcc atgatctgct cacagacttc
     1741 ttccattccg gtgaaggctt cagcctgcat ttgagccagc tcgtcagctg acggtcgctc
     1801 aaacttagcc aggaacttct gcgtcttatc gcctatctgg cgataaagca ccgtcaggta
     1861 caagtcatta accatctggt tagaccccgt ttgcaggcgg cggttgtatt catccaggta
     1921 gcgcgggaag aattgggtaa actcagagtc aggatactct ttagactggt gatgatactc
     1981 atgtgaccag acctctacgt ggtcagtccc gaaggactta atcaacgtat taagatcatc
     2041 gttccatgta atcagctcac ggtcagaggc acaatcatgt gtccggccat cgactttaaa
     2101 gaatgcgagc aaatcgccat tacgcatcga gataatataa tcatcaaggt gctggctatg
     2161 ctgcaaatac ttttcattaa cgctcggttc gtctctgaac gttttaggtt taagcaatgc
     2221 caccatttat ttcagctcct tttttttgta atccacgggg gaataacttg aaccactcca
     2281 ttgtttaaat ggcgtgtcgt gtgcattacg aaacttagtc tgtagccata atgaaaggat
     2341 gcgaaacata cggtcgtcgt atttagttag cgctgctgca attgaccaca gaaccgcaaa
     2401 tacaaggagt gaccataaat gtaacgtcat aaacagcgtg gccgagatca tgaagataac
     2461 cagcgccact gtacgtggaa cgccagcaag ccgggggacg cgtgttgcac ctttaaataa
     2521 tggccgttgt ccattttcaa acataaaacc tcacttaatg ggggatgtac tcccccccat
     2581 taatcagctg ccgataccag tcagggatac cagatatgat gcggagccaa tgccaaccat
     2641 tgcgattgcg agacggaaca taaaaccagc ggaaataaga tggaacagcc aaacgaggaa
     2701 cgacacgatc acgccgatgg tacaggcgat aggaatccag gtatccagcc atgacttgat
     2761 gttgttacct gtactggccc cggtgtctgc cccgccagct gcgagggcca gttctggcgc
     2821 tgcgatacat aacagcgcaa ccagcagcgc aaacccgtta tttttgaaaa aagtaaccat
     2881 atatacctct acctttcctt tgttgatttc aatatcaatc ggcactcgcc gacattgcac
     2941 ttatccagaa gaaactccgt caggagtctc aggtctgccc atgtgcgaag ttcgtgatct
     3001 ttattcaacc gaatggagca acgaacctca cccaaatttt cgtcctttac gtcaaatatg
     3061 ggataccact gctttagtga aatcctttca attacaacct cctgtatttt tccggcttcg
     3121 cataaatcaa ttatttgaga ctgcaacagg agatagattt ttgtcattca taacctcact
     3181 ccctttttgc tctgctttgg tcagaaacgc gtcagtgtta tcctgcgaaa acacatcacc
     3241 accactatca ctttcacccg taaacgcatc gtgctcaccc tcaaaggttg cgcccttcgc
     3301 actcacaggc tggctctccg cttcctcgtt ctgcccatca ggtagcaacg tagggatctt
     3361 taaatcagac tgtttagcca cgttaataac tttcgtgaca tacccgtttg aaatcccccg
     3421 cacttgtgac ccggtgttgt agcaggacag cgcgtgtctt aatgctacct gaccgggcgg
     3481 gttggatttc agggccgtac tataacagtc ttttaaaatg gtctgactgg cgcggagatt
     3541 gatgcagggt ttaaatacct gctcaaccgt gatacctaac gaactcaaat tgttagaatt
     3601 aatcatcgca agccccatat cgatgctttc atctttcttg aggtttttga ttagccggtt
     3661 ggcaacttct accgcttcag cttcggtttg cggttgacgt ttcaggtttg cgccacggtt
     3721 aacgttaata aaatacgggt tgttcccgga ttcatggccg acgatatagg ccattgttag
     3781 cggtgaaacg tctggtgcgc atcgggcagc aaggctggcg atcgtatccg aggctgaagc
     3841 cgcgtgagcg accccctgaa aagaaaggcc ggagatacat accgctatca agcaccatga
     3901 ttttttcatt tttgtatgtc cgcgtaattg taattttgac gacttttatt tatcataagg
     3961 gccaatagaa tcgtgtcaag agaaaaagac gttaaaggga agaaacctat gcttgttcct
     4021 ttgcagtcgg agaagcgacc aaaagggacg cctgtatacc gtgacccgga gaatccattt
     4081 aatacctgga gcgggtacgg aaagcgccct gtctggttac aggaacgatt gagtgatggg
     4141 tatcttctgg atgaaatgaa gctgcctggt gcagtggtgg atcgggatac caaatccact
     4201 cgataccgtg acccggctaa cccggataat acgtggactg gcgttggccg tcgtcctaag
     4261 tggttggttg agctgctgga aaatggtgtg gatttggacg atctgaagat tcgagaaagg
     4321 gagtgaacga tgggcaatgt gattgagttt aagaggccga aaggcgaaag aaaccaaaaa
     4381 gacaaaatgc ctaaaagggg gatactgctg tggttcaaaa agactgccct gtccctcaaa
     4441 aacaacctct ttacctactc taagtcatgt tgctgtattt gtttgttttt tgtaagcgct
     4501 gcgtcaagaa agctattgaa gttgatcgca acactatcaa tcgtgctgtt tcttgtcgag
     4561 tggtttatgg gccgtttggg agcgccagag ttctataacc tgttgttcct aatgggattg
     4621 ctgttggtta tcaatgtgat ttcaacaagc tgtatcaaat ggataagagg gaacgtatga
     4681 gtaagctaag aaccgttgca aaaatattat tgatgtactg catcttcagt agcgccagcg
     4741 cctttgcgga gggaaatact aagggtgatc cgtgtctcgc gttcacctgt atggccggaa
     4801 agttacaagg taacgaagcc acatctggcc ctgactgtgt aatgggccgc cagagcttct
     4861 ttgagatacg aatttacacc ccctactaca accccggcgc gaccgctgcg gctcgtaaag
     4921 tatttctaag cagctgccct atgaccacta tttcagaaaa caaaaacaca ctggagctga
     4981 ttattcagcg atacggacgt tcatttcagg agtaacacat gaagaaacta atcccagcca
     5041 tcattgccgc cgccctgctc tccgggtgcg ccactcagaa cgacgcggta gcgctctcaa
     5101 accgaatcag cgatcagcag aagcaaatca acgcgctttc cgttcgcctc cagtctgccg
     5161 agcatcgctt gagtcagcag gagtcggctt ttcgctcaga acaaacgcag aacagcagct
     5221 actgctacct gaacggcgtc aaatactctc agggatcggt gttcgctggc cgtatctgtg
     5281 atggtagttc cggcagtgcc gcctggcgtc tttactccca ccgctaataa gcaaggcttc
     5341 cgggcttgcc ccggaagcgc ttcctccaga catggatatt gagctgcaca atccatgttt
     5401 atcacaacaa tcatattgtt aaagaacata aaaaagtcgc catgaaggcg acttaaacga
     5461 gctggaatcg tgcatatttt atttttgtaa gtgccctggt gctgtattca gggtgaattt
     5521 ttgcactata taggcactat ctgcaaccac tttaatctat ctgacagcca catacactgc
     5581 ttgcacctgt aactgtgtgg aaacggatgt aatggccgaa ccctatttaa aaaatcgccg
     5641 acgcttcacc tcgtcccttg ataatcagct tgtcccattg tttgacgctt tagcggccaa
     5701 aacgcggata cctaaaagcc gcctgctcga tgaagctata gaggatttgc tggtcaaaca
     5761 cggcgttcct ggtaaaaagg tcgatagcca gaattaactt ttgctcaccc gcgtaaccgt
     5821 ccgttccgat acgccagcga gtctggccgt ctcgctgata gatttcccct ggtgccgcaa
     5881 agaacggatc aggtcatgct tattctggtc gggcatccgg ccacgataaa cccccttctc
     5941 tttggctttc cttacacctt cctgctggcg acggcggcga tctgtatagt cttttcgggc
     6001 gatggccgcg agcatatcaa gcatcatgct attgatggcc gtcagcatac tccgtgtaaa
     6061 ttcatcgcta ccgtggggca ttaacgccgc gtagctggtt ggcaggtcca gactgataat
     6121 cgccaggttc ttttcctgca tcattcgctt cagctgctgc cagccttcat catccaggcg
     6181 tgaaaggcgg tctacctgct caatcaggat aagatcgccc ttctcagcat catttagcag
     6241 gcgcataagc tcagggcgtt gcagggacgc gccagacgca ttctcaacgt accatccggc
     6301 tacccggtga ttatgctcaa gtgcaaaggc tttaagccgc tgctgggcgc gtgacgcatc
     6361 ctgatcgtgc gcagaagccc ggaggtatcc aaatatgtac atgaccgcct cttatgtcat
     6421 tagggttatg tcactataac ctatgtcatt agagttatgc cattaggcgc gaggggaagg
     6481 attcagaggg tgacagaagg gtatacccaa aagacataat ggtcttattg ccgacaatgt
     6541 aactctgttt tgattccaaa aggatgaaag catttatcgg gttgcagctt tggactattt
     6601 tttcaaccat tatattactc tacgtgttat agagcaaaac tcacatatca ttctaatata
     6661 gatggcgtta ccataattaa tattgcataa ctcattattt gcaatattaa aattgtggct
     6721 cttattgtca gagtgttttt atctgaattt ttatactcat gcttatcaag gagtcgtaag
     6781 aatgcgagcg ttgccataaa cataagaatc aataagccta taacattaga attgaacgcc
     6841 tgcgccagag taaaaccaac ctccctaagc agaacatata tggataacga aattatatat
     6901 atgagagaga agattaatat tggtttattt gacagtctgt ataatttttc catcattaac
     6961 acctttattt aatgattttc gttttccctg ccagcacgta gccaactggt cattactttg
     7021 aatttgatcg tgacagtcac gatgtgctac gtatcgcttt tgggtttaac tggttccaca
     7081 ggcagaaaaa aacgcttgcc tccctgcggg gatatgtgag tgatatttgt accatcagat
     7141 cgcttctcta ttaccgtgtt acggctgtaa tcatagagcc aaactgtgcc tgtactttga
     7201 aaaggattaa ccgaaacatc aatagtcatg ccttctctgg ccttccctac tgcacaaaca
     7261 tttctattct gctgacgttc tgtcattgcg tcctcctgtt gaaattaata ctaccgtaca
     7321 accaatccca gtcaaagtaa atgctagtca catttatttt tactggtaat tactaaagcg
     7381 ccgtgctagt gttttctcag cgttacatta ctacggaaaa tgacaatggc agaggacagc
     7441 aaacagctaa ccaaacggca acaaaaagcc attgatacag cggcactaat ccggcaagaa
     7501 ccgccgcagg gtgatgatat ggcgttcacc cactctattc tgtgccaggt cggtttgcct
     7561 cgctctaaag tggaaggccg cgagtttatg cgccgctctg gtgatgcctg gcttgttgta
     7621 caggctggat ggcttgatga aggtaatggt ccggtagagc agcctctacc ctatggggct
     7681 atgccgcggc tcacgttcgc ctggatttca tcgtatgcgc tgcgcaacaa aacgcgggaa
     7741 atctccatcg gccacagtgc cagcgaattt ctacgcctta tggggatgga acttcagggg
     7801 gcaaggcaca gaaccttgcg tacacagatg caggcgctgg cagcatgtcg cttacagctg
     7861 ggcttcaaag gccgtaccta taacggccag ccagtcgaac agtttgatgc ctggctgaaa
     7921 gatggagaca caaagcagct tacgttatgg ccggggacac tcactctgtc agagggctat
     7981 tataacggcc tgattgagag cgccgttccg ctcgacaaca gggcgttgca tgtgctgaaa
     8041 ggctccgctc tggcactcga tatatacgca tggcttgctc accgtctcca ccgcatcgag
     8101 ggccgcccgg tcatgctgta ctggatgaag ctacgggagc agttcgccca ggagtacagc
     8161 gggaagaatg ccgacaagga ttttaaacgg gcattcatgc cagcgctgaa acaggtgctt
     8221 tcagtgtatc cggcggcaaa ggttgatcag gtcaaaggtg gcctgctgct gtacagttcc
     8281 cctcccccta taccgtacaa atcgtgaaga aaaggcccgg actgggcctt ttcttttgtg
     8341 gataaccctg ttcattcagc aagttatcca agataaatta ccccccctaa accgagataa
     8401 attacccccc ctagcaagat aaattgcccc cctaaccgag atatatcacc ccccctaaac
     8461 cgagatatat tccccccccc ctaaacctgt aaggtgatcc tgtatgtttt tcctttaatt
     8521 tctacctata catatatcct gtaattaaca aagcctatct gtgagtaagg cacgtccggt
     8581 gtatgactta gcaacagtgg aaattacgct tcctcgacct gacagactcg ccgcgctgcg
     8641 gctccctaat caggtcttgc ggcgagcgtt gcggactctc ttttaaaagc gataactttc
     8701 gctataatcc agagctatca gcatttcctt cgctgattca cccggaggcc agttgctgcc
     8761 gcgacggcct ccccctctcc ttctcattag ttcaatagag taaaatcatt atttactaac
     8821 attgaccgtt agcggtggcc gctatatcat gggcttaatc gttatcaatc aggaatgact
     8881 atggcccgca aacagacccg cagcatgacg caggaagaat gggatcgcct cttacccgca
     8941 atgcagacgt ttacccactt atcgaccgaa atcggtcatt cagtgttggt taaaggggaa
     9001 agcaataaag acgtggccga acgtgtcgga cggaccaagc agaacgtggg ttccacggtg
     9061 aagcgcattt gggatctgta ccagtcgtta gccgtcgata ttgagggcga aaagctacgc
     9121 aaggttgacg tgtggatacc tgaaaaatta gccctgaagg tgctgaagga agcagagaag
     9181 tacgccatca atcagagcaa agttgaacaa tccgaataag aaataatagt aaatatatag
     9241 tttacttaga ttgactatta cccttcaaat tgcgataatt atttctccgg gaagctcaca
     9301 gcttcccatt atcaggagga taaattatgg cttcacgcgg tgttaacaaa gtaattctcg
     9361 ttggcaattt aggccaggac cccgaaatgc gctacatgcc taatggcggc gcggtcgcta
     9421 acctgcgcct tgctacatcc gaatcatggc gagataaagc cactggcgag cagaaagaag
     9481 tcacggaatg gcatacggta gtcatcttcg gcaagttggc cgaggttgca ggcgaatacc
     9541 tccgcaaagg ttcgcaggtg tacattgaag gccagctgcg tacccgcaaa tggcaggata
     9601 aggaccagca ggatcgctac tcaacagagg ttgtggttag catgaatggg gttatgcaga
     9661 tgctgggcgg tcgtcagggt ggcggtgctc ccgctggtca ggggcaaccg caacaacagt
     9721 ttagcggtgg tggtgcgcca gctgctgcgc cggggccgca acagccacag caaggcgcga
     9781 tgccagactt tgacgatgat attccatttg ccccggtcac actccccttt cctaaccatg
     9841 cgattcacgc actgtaatga aaataaagcc ccgtcagggg cttttcttat gagggctgaa
     9901 gttcggcctt ctcgcaatgc tccccgccgc agaacacgac cagacccggc ttaccccaca
     9961 gattcacatg acacacaggg caggtgtatt ttgcgcggtt ggacttgttt accggggttt
    10021 ctggcggcgg cgaaacaaac ttcattgcct ctatgatttc attatccagt tctccgggtt
    10081 cgtcggtgta gtcgtcgtcc ggcgggtatt ccccatcatc atcttcatca tcgatgtaac
    10141 cttttccgct gggtgactgc ggcgctccgg gcttcggctg gtacggagga aagcggtcca
    10201 tccaggacag ctgaaaattg cgtgttaaca gttcatcaca cgcccggtca aaagcgccgc
    10261 cggtgataat gtagtgcgtc atgctttggc ctactttccg ccctccgggt tgcccggtat
    10321 ccgatggcat taaccctacc cgctccatta gcgcggccca ctctttattg tgatagccac
    10381 gtctcccggc cttgccaaaa tgaaactgcc actggtgaac catttcatgc accagggtag
    10441 ataacgtcgc cttgatggtg cgtatggaga aatacaccgg attcatggct atttcatcga
    10501 ccatttgccc gctctcacgg cccgcgaagc gctgaaatga gcaatagcca taagtacgct
    10561 tttcacgttg cagggtgatc aggcatggcg gcagctcgtt attaaaaagc cgttcatcaa
    10621 aaaaatcgta ggcgtgctgg agttcttcgt aggtttcggg tgttggcaat gtcatgtggc
    10681 cttccttctg tattgcgcaa tacagaaaat atacttccgg cagagagtaa agtccaacaa
    10741 aatttgtatt tactaatatt gactttgtga gtggaaaaag cgatactaat ttcatcggga
    10801 ggcaataccg ccccgttaac acagcccaaa gcggcaacct cggagtaaat actatggcta
    10861 atatcgtcac tgcaaaaatc tatggccccg ctcagaaaat gcgttttctt ccttacgcgt
    10921 tcgcagaatt ttatcttcag gcagaaaact acgtttgcag ctttatgaaa aaatacgcag
    10981 ttggctatga tggcggctgc tgggagtaca tcacttatga aaacggcgcg tgtgcgttca
    11041 tcgcgccgga tggctaccag cttacgctcc ctaactattt tgatgaacct gttagcgcca
    11101 aagaagccgg gattatcgtt gcgctgtatg cctacagtca cttttgctgc gtggcctttg
    11161 agcgtggctg gcagcgcgtg aatgagacga tggccgagcg ctatcacacg ctgcgggact
    11221 ttaccgaaac actcccgccg gagtcacaga gccgtatttt tcgggcaata gattaaccag
    11281 gagcggcggg catttgcccg ccttactcgt tcaggaagaa atgctgatgg ttacgacaga
    11341 tgaagtctgg tttcggtatc tcgactattc gggccaaaca aaggcggtcc gggtggcatc
    11401 cgtgcggttc tggccggaca ttcaggaaac gatattcccc cctctccagg tgccggaagg
    11461 caagcgccgc gttgtacgct gccgctgcgg cagtaatgac tggaatgagg atggccgctg
    11521 gctgggtgaa tactgctgcg ccagctgcgg gcaatacata caggtatttg agaaaaagga
    11581 ctgacatatg gctttcgact tcaaaaaaga ggatgcggcc aagtatggcc gcgaggttta
    11641 ccgggcgttt cgtggtaagg gcaatcaccg ctgggatacg tgcgttttcg tgaatgaatc
    11701 cggggcttac tctgccgttt tccggcactc gttccgcaaa aaggttatcg aggatggcaa
    11761 agagatccgg cgcaacgtca ttgatgatga aatcgtggtg gccgcgccgg acgcgggcag
    11821 ctttaccagg gcaaaattcc cacaactggc cgacgcaaaa gagctgaagc aatcaggctt
    11881 tttctcccgc ttacggtttg ccgctgaagc agctgcttat cgtgaagcat ggccggggca
    11941 tgatggcggc gtggtgctga tatgggaagg gaaagcctat ggctggaaaa attgcctgcg
    12001 tgatgccggg ggtgagcgcc ccggcgcgat agcgatcgac actgacggcc atgtttttat
    12061 tgctgaaggt ggaaacgagt atgatggtgc aaaatgctgg gttgcgatga ttgacaggga
    12121 gaatgaaaag aatggttagt ttgttttatg ccgcaagggg attatggcgc gttgttgcaa
    12181 actttatccg tgaagccgtc cgtagttcgc tacttgccgt tatgcacatg gttagtgtcc
    12241 atatccccgt actcattttt ttagctgtgt tcgtgttggc cttcagtggt tttgggcttc
    12301 tggtgatcaa agtattagct atcgtgttgc accctgtaac gcaggggcca atagtgggaa
    12361 gtattgcaaa cgtgctggat gctctgaatc aggctctttc gccaatggta tattttaaaa
    12421 ttgccgtacc tgtctttttt gtggtggtcc ttacatcact gtttggctcg gttagagtca
    12481 actggaaaaa gatagtgacc agatggcaga ggctggttga ggttgggaaa gataagcgtt
    12541 gatttgttaa agggcgggga gtggtccccc cacccttact tacgtttccg gtcagtcaaa
    12601 aaactgtcgg atcagttcca gaacagctat tacagcgtta gacaccgcaa caaccgctcg
    12661 cagaacgacg attaacacgt taatcatccg tttgctccat acagagccgg aacgtcgctg
    12721 attgccctta cattgcctgt cacagcgact acccaacgtc aattgtttgg tcgttcccgc
    12781 tccagcctgg gcattgaaac agcaccacct gtttttcaac caggacttta atccgttcat
    12841 gccgcataag cgaggagctg gagcgaggac gtcaacagat tgacccgcgc agtataccct
    12901 attccgcgct gtttgcattt acgctaccca tgacgtaata tttcgttgtc aattgtttgg
    12961 tcgttcccgc ctcagtctgg ggcaaaacat taaagcccga tctcaccagt cgggtttttt
    13021 tgtgcctgtc gtttaccttt ccgcgctggg cgtcgccctc cgggccgcta cgttgtgctg
    13081 cgcatcgagc cagccttgct gtctcgcccc ttcgggcttc catcgctgac gcatcaaaaa
    13141 tactgaatac ctgtttttcc tgccttctgt cgctaactca ctgaaataac gcaaatcgta
    13201 aaatgggtgc ccgccagctg cagctgcttc tcagctgctg ccggctgcgt gtgtcgttca
    13261 gcaaatttga tcgtatcggc cagcgtcacc agcggcgctg taagcccgtt tacagcccat
    13321 ccgggcgcgt cagctgcgct gacccaccac ggacccggaa atcacgccca gcgcgtcgca
    13381 gagcgccgca caggccgctc accgggacag ggctaatgtg tggccgtggc gtgccgttgg
    13441 gggcggtgga aaagagggct ttgcagggag ggggattatt acggctaaag ccgtaacgct
    13501 ttttaagtcc tcgcttcgct gcggggcttc cggcgcgttg cgcctcccat gcgctccgcg
    13561 catcttctta actgccgtag ctacatgaaa tatatgtaat aaatgtattt actaatattg
    13621 actttgaagg caaaaaaggc gatacttata ttcatcggga ggcaatgccg ccgcccggta
    13681 acacagccca aagcggcgat tatcaggagt aatattatga ctactttaac tgtcggccag
    13741 tgcctcacct cttttaacaa cgagtatgta gtaagcgcgg ttaatctggc tgacgacaaa
    13801 atcagctata ccatcctcgg cctgaatgcc ccgacctgcg ccccgctgct ggaaacctcc
    13861 ctgcgctttt accaggtgat cgacaagacc cttccactcg atgaactccg cgcccgccgt
    13921 caggttgtgc agagtgtgac cgaccagcga gaagcgcgac accaggcgaa agaagatgcc
    13981 cgccagctgg agaacgaacg cgccagtgct gacccggaga acgccggatt gctgaccacg
    14041 gccaccgaaa gcaacaccac caagctagcc gctaaaaaca tccgtattct gctgaaaaag
    14101 catttccccg gcgtgaagtt ttctgtccgt atgcgtgact ataacgcgct ctatgtttcg
    14161 tggacggatg gcccaaccaa agaagctgta gaagcgatta cagacaagtt tgaggaaggt
    14221 agcgttaact caatggagga catgtacgaa tacaacatta cgggcttcca ccgtgtttat
    14281 ggtggcgtga aatacctgtt ttgctcacgc gacctgaccg acgcgctgat cgctgaatct
    14341 atcgaactgc ttcgcaaaga atacggcgaa accaccatcc cggctgatgt gacgcttgag
    14401 gcgtacaaat ccggggcgct ggcggggcgc gggcatgact gctttacctg ggggcttgca
    14461 gcacaaatcc gcatcaatgc gggaaaggtg gataaatcca gccgctgaaa ttagcgccct
    14521 tcggggcgct ttttgttggt agaatcgaat gaaatatatg taataattgt atttactaat
    14581 attgactttt aaggcaaaaa aagcgatact tatttcatcg ggaggcaata ccgccgcccg
    14641 ataacacagc ccaaagtggc atctaaggag ttatctatga actatctgat catcatccca
    14701 atcgcgttta ttctcgccgc cgccgcgctg gcccttccgt ggtttatggt tcgccagggc
    14761 taccgcgagc aagtccagct gggcggcgcg tgtactaccg tgctggggct ggccgcatca
    14821 acaggcgtgt ttagctatgc cgataatatg ccgctggcgc tgctgtacgg ctctgcgtcg
    14881 gtcgtttcat tcctgtatgc gcttgattgc ttcctgccgc tttacctgtc acgtaaatct
    14941 cactaaccca caggcggggg gaaacccccg ccagcctgga gaaaaaaaat ggaaaacgga
    15001 aaactcgccc agcaacaggg ttttgacctg gcgttgcaga tcctgaaaag ccagagaggc
    15061 tattacattg gcactgcgac cagtgagggc tacccggtat caagggaatc cgctgaatac
    15121 ttcccggaca gcagcactgc gcagcaggct ttgacggata accgctggac acagcgcgag
    15181 gattgttaat taaattgcag ggcggctaat cgccgccctg cataacgaaa aggaggccgg
    15241 acgatggctg atcatgacga cgcaccggaa aaaattaagt gcctggagtg cgggaaggag
    15301 ttttctttcc tggcccctca tttgagtaaa gcccaccaga tgaacgccag acagtaccgg
    15361 gaacgctggg gcataccact acacacgccg cttgccagcg ccgagcattc ccgccagtgt
    15421 cgggaaaatg ttttgcgccg tatccggcgg ggcgaaattc gccccgctga ccagctcgcg
    15481 ctcatggccg aaggccggaa gaatgctcct gaacgtgcga ccagcaccag gttgcataaa
    15541 gtggcggcgg cgaacgtggc gcgggtgcat cagatatgga agcattctcc ggtggtgaag
    15601 gtcgtttcag atacgctcag ggatgaagcc gtgcagagaa tgacggccag aaaggtgact
    15661 ggtgaaaaag ttaaggatat tgccgccgac ctgaatctgt cggttgggtg tctgtacaaa
    15721 tgggtggcgg acgctaaata gggcgtctcc ctccgggccg ctacgtcgtg ctgcgcatcg
    15781 agccagcctt gctgtctcgc cccttcgggc ttccatcgct gacgcttgtg ataaacatgg
    15841 attgtgcagc tcaacgggta tctgctcgtc aaacccggcg cttgcagcgg tcaaaggcca
    15901 cgccgcgcca accgccccgc aacggggcta taaggcatca aaaatactga atgcctgttt
    15961 ttcctgcctg tcgtcgctaa ctcactgaaa taacgtaaaa cccgaaatgg gcgctcgcca
    16021 gctgcagctg cttctcagct gctgccggct gcgtgtgtcg ttcagcaaat ttgatcgtat
    16081 cggctaacgt taccagcggc gctgtaagcc cgtttacagc ccatccgggc gcgtcagctg
    16141 cgctgaccca ccacggaccc ggaaatcacg cccagcgcgt cgcagagcgc cgcacaggcc
    16201 gctcaccggg acagggctaa tgtgtggccg tggcgtgccg ttgggggcgg tggaaaagag
    16261 ggctttgcag ggagggggat tattacggct aaagccgtaa cgctttttaa gtcctcgctt
    16321 cgctgcgggg cttccggcgc gttgcgcctc ccatgcgctc cgcgcatctt cttaactgcc
    16381 gtagctacat gaaatatatg taataaatgt atttactaat attgactttg agggcaaaaa
    16441 aggcgatact tatattcatc gggaggcaat gccgccgccc ggtaacacag cccaaagcgg
    16501 caacccacgg agtcccaaat gaaacaacat atcgctgcaa ttattcgcga atacaacacc
    16561 cctacagtaa ccgtcgaagt agccaataca gaccgctacg atagcgagca aatcgagatc
    16621 cgccacgttg ttgatggtcg ccttgcctgg agagcgtggg actatgaaac aggttttgaa
    16681 aatgacctgc atcgcgagct ggcctattac catattccgg cctgattgag cataaacacc
    16741 ccgcccggtg aaagccgggc aatacagccc aaagcggcac ttatcaggag taagcctaat
    16801 gacaaaagtc acaacaccgt cccagctgaa agcagagctg gagagccaga aaacctacct
    16861 tctcgaagcc tgcctgatgg cctttaatca gttacctaac cagcgtacaa aaggcgcgtt
    16921 cccgtcaaca tacgcgcttg cagcaaagat tgatcacctg ttgcagcagg aaaaaaaata
    16981 aaccttccga caacgacaaa gcccggtatt aaccgggctt ttctgtattg cacaatacac
    17041 taaaggttaa gcgcgtttgc gtcctgcgcc agccagcatt ggtatgtttc cttttgaaac
    17101 tccccgccgc agccgccacc ggcttttgat tttccgcagt gctggcatgt acccagcgcg
    17161 gctttttctt gctcccactg ttgccagttg cggatcagca gcagctggaa aaactcattt
    17221 ggtgaatagg gctgtttacc catccggcgc acctgccgta gttcgtccag ttgcgccgct
    17281 tcatcgtgcg ttagcgtgac ggtcacgcta acgccttcag cgcgtaaccg ttcgcggcgg
    17341 cgagcttgtc gccgtctggc taacatcctt tcgcgttcag ttttatccat ttttatcaca
    17401 gtgactgtca cgattgaaaa gaaagaaaaa ggccgggaat ccggccttgc gtgttacttc
    17461 aggatcagca aggtcagcgc cgtcccgatg gccgcgccga cgatggaaca aacagccgtc
    17521 atgatcacga cgcggttaac gatggtgctg gcgttgttgt gctggagaga ttgccagcgc
    17581 gccagaaagt ctgaccgcgc cttgttttcc tccttcatcg ccgcctgtat atctctcagg
    17641 ctgccgatga tttccgctct cgaatcgtct gccgtcaatt caagtgcgct tttgagttcg
    17701 cccagctgct gaagcatgtc ggcttgttcg gccttcatta acttcatggc ttctaaaatt
    17761 tccttcgcgt ctcccaaaaa ccagtccatc aatacgtcta atttatcggg ctgcttatcc
    17821 atgcttaatc tccgattaac agggagaatg ttttatccag gttgctctta accgggatgg
    17881 cctgcttcat gtaagtataa cgacgcgcca ttttgcggcg ttcgtctgcg tcagctgatg
    17941 ctttagcgcg ggctttaaat tcttccgcat cttcagcggt cagcgtttca aaagaaattt
    18001 tatggtgtgc aagatattcg tacacctcac tattatagat cgcagagttt ttattaatgc
    18061 ggcctacttt agtttgtttc acaaagtcga aaatcgggcc gaaaacattt tcaacatcat
    18121 cacccggatt aacgcggttt ggaacaaaga taatcttttt gttatccacg ccagccttgc
    18181 ttaacgtgtg cgccgttttc atactttctt taatggcttt atcttccggc gttaccggaa
    18241 tgaaatacat atcaaattca tttgcgccgc catcgaagcg ggacattgcc attaaaaacg
    18301 gttccacgtt tgacgcgcca atatcaataa tcgctgcatc ttcaaaaacc acatcttcaa
    18361 tcagacggga gaaatcatca cctttaaaac tggtgacatt ttcaatccct aagtcagacg
    18421 ctgattggtt aatggtttcg accgcgaaaa atttcgcatc tttcaggcgc ggggcgagaa
    18481 ggtaagttga aactaaagtt ttgccaacgc tgccactgta attaataacc gctactttca
    18541 ttgcacacct caatattttt tgatgaactc attaggatcg aactcggctc ttgcctcgtc
    18601 aaaaaattcc tttccgatga tacctggtga ctgtttcgtt tcgtctccct cggtcgcact
    18661 ttgtgtattg tgcaatacat cttttttcac ctgctcagac ttcgcgggtt tttctgtatt
    18721 gtgcaataca ggtttcttct gttgctcact ggctccggct gattctgtat tgtgcaatac
    18781 agaatcaggg gtggcatgtt ttgcccggtt ttgcctggcc cgatacaggg cagtgtcaaa
    18841 acttcgaacg ttcacattct ccaggccgaa gcgttcagat accgccttga aaacctcctc
    18901 tctggagact cccagcgaca gcatttcttc tacttccggc aacatcatct gaattgctgc
    18961 cctgatactt ttgggcttca tagtaccctc atgtagaatg atgtattttt gatgtaatag
    19021 tgatgtattt atgtcgtatc aactatccca tgacgtaaga gtgatgtaat tgtggtgtaa
    19081 tgtcaaccat gaattgacgt aatagtggtg tatttgtggt gtattggtat tcatgcaagc
    19141 atgaataccg ggatgttgaa cgctttttca ccaaaatacc ccttaacctc cgtagatcgc
    19201 ccctaccaaa ctgattattt tttagccctt aacctgctat acacctaacg cagtgcgatc
    19261 aatcgcacta actaactgat ttaaaaaaca aaaaacgcaa tattcctgat tgtgggtatt
    19321 aaaaaggtat taaaaaaata tcataagagt atggtttata tggaaatgta ggtattactt
    19381 tgttaatact tatctcatgc ggttaaacta ccgccagatt aaatacgaag aatcaccagt
    19441 tgaggacata atgccgatca tcacatgcaa ggtaacggac gaggaaaaag agcttatcga
    19501 caggctggcc ggaacgaaca gatcggacta tatgcgccgt tgtttgttgg ccggatcagg
    19561 tggccggata ggtggattga gccaggttac ggaacagata aatgaagtaa accgacgcct
    19621 cgatattctg tttgatcagg tatcagagac agacatgagc gcgttttcat cggagctgaa
    19681 aggcctggtc aaacagctgt cacaactcaa gttgccgcct cccggtcaga tgatgctgca
    19741 tgaatcgctg gcaatcgaga cggtgatcct gctgcgttct atggcgactc cagccaatat
    19801 gaaagccgcc tggggagagc tggaacgtaa cgggtacaag gtatgggaaa gtaaataagg
    19861 aggaaggaac aatgactgaa caagaaagag gaattatatt tttatgctcg ctgctggggc
    19921 caataccctt tgtgtggttt attactgcaa agtttgttta tggtattaac agtgaaactg
    19981 cacactatct tgtcccctat ttgctaaaaa atacgttcaa cctgtggccg ttatgggcgg
    20041 cgattattgc aggcgcggtt gtaggtttgt gtggtttcat agggttcatt atttacgata
    20101 aaggaagggt gtataaagga ccgagattta aacgtatact ccgtggcact aaattagtca
    20161 gagcttcatc tttggccgag aaaacaaaag aacgcggtca taagcaatta acggttgcag
    20221 gtgtt
//
