LOCUS       NZ_CP019843             4667 bp    DNA     circular CON 11-APR-2024
DEFINITION  Enterobacter roggenkampii strain R11 plasmid pASM4, complete
            sequence.
ACCESSION   NZ_CP019843
VERSION     NZ_CP019843.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06323809
            Assembly: GCF_002007805.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Zhong,C., Cao,G., Zhang,C., Jiang,T., Chen,W. and Zong,G.
  TITLE     Complete Genome Sequence of Enterobacter cloacae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Zhong,C., Cao,G., Zhang,C., Jiang,T., Chen,W. and Zong,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2017) Shandong Medicinal Biotechnology Center,
            Shandong Academy of Medical Sciences, 18877 JINSHI ROAD, Jinan,
            Shandong 250062, China
COMMENT     ##Taxonomic-Update-Statistics-START##
            This Genome (query)               :: GCA_002007805.1
            Current Name                      :: Enterobacter roggenkampii
            Previous Name                     :: Enterobacter cloacae
            Date Updated                      :: 2019-03-07
            Analysis Type :: Average Nucleotide Identity (ANI)
            Analysis 1 (A1) :: Query vs. TYPE genome for current name
            A1 Genome (subject)               :: GCA_001729805.1
            A1 Name                           :: Enterobacter roggenkampii
            A1 ANI                            :: 98.6683%
            A1 Query Coverage                 :: 88.48%
            A1 Subject Coverage               :: 90.16%
            Analysis 2 (A2) :: Query vs. TYPE genome for previous name
            A2 Genome (subject)               :: GCA_000025565.1
            A2 Name                           :: Enterobacter cloacae
            A2 ANI                            :: 89.13348%
            A2 Query Coverage                 :: 79.89%
            A2 Subject Coverage               :: 71.25%
            ##Taxonomic-Update-Statistics-END##
            ##Genome-Assembly-Data-START##
            Assembly Method :: Newbler v. 2.8; other v. Jan-2017
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 181.0x
            Sequencing Technology             :: Illumina MiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002007805.1-RS_2024_04_11
            Annotation Date                   :: 04/11/2024 13:33:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,807
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,619
            CDSs (with protein)               :: 4,619
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 20 of 74
            Pseudo Genes (incomplete)         :: 58 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            Pseudo Genes (short protein)      :: 1 of 74
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019843.1.
            Bacteria and source DNA available from Dr GC at Shandong Academy of
            Medical Sciences.
            The organism was changed from 'Enterobacter cloacae' to
            'Enterobacter roggenkampii' in March 2019.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="R11"
                     /isolation_source="sewage water"
                     /db_xref="taxon:1812935"
                     /plasmid="pASM4"
                     /geo_loc_name="China:Shandong"
                     /collection_date="2016-12-17"
     gene            965..1288
                     /locus_tag="B1H21_RS24230"
                     /old_locus_tag="B1H21_24530"
     CDS             965..1288
                     /locus_tag="B1H21_RS24230"
                     /old_locus_tag="B1H21_24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1497..>1931
                     /locus_tag="B1H21_RS25115"
                     /pseudo=""
     CDS             1497..>1931
                     /locus_tag="B1H21_RS25115"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1966..2451
                     /locus_tag="B1H21_RS24865"
     CDS             1966..2451
                     /locus_tag="B1H21_RS24865"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_045326458.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            2455..2670
                     /locus_tag="B1H21_RS24870"
     CDS             2455..2670
                     /locus_tag="B1H21_RS24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015589619.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3016..4572
                     /locus_tag="B1H21_RS24240"
                     /old_locus_tag="B1H21_24540"
     CDS             3016..4572
                     /locus_tag="B1H21_RS24240"
                     /old_locus_tag="B1H21_24540"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013199864.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_048290311.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLD
                     ANSSYAMKMSDVMSGYIEMAQGELSYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVFV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLSHPIYD
                     KNGNYIGYVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGTVIFNNDNSAVGRPMV
                     MSDDLKSKLTESDRGEGEFFFSGNRYLLGYAHMKNTAWNVLVYSHADSATTILITYAKS
                     VLVLLIVIIITFSLLSYFIASRISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTHVKVMMERVDSLNEVALKDPLTGVNNRLGFSTKIQNNKYGAGASVIAIDVDFF
                     KKINDSFGHGVGDEVLVSLAQIIKSCCRSEDIICRFGGEEFVVFLPNTSVVTAECVAER
                     IRSVIERTVFPNNLRVTVSAGVATQDDPLGGIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     Typing          680..852
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.126"
                     /coverage="100.0"
                     /evalue="1.99e-77"
     Typing          complement(203..755)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.639"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          966..1282
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="2.47e-61"
     Typing          1279..2774
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcgtaatctc atgccctgca aacaaaaaaa ccaccgctac caacggtggt tcgtttgccg
       61 gaatcaagag ctaccaactc tttcttccga aggtaactgg ctttcacgga gcgcaggtac
      121 caaaactgtc cttccagtgt agccgcagtc aggccaccac ttcaagaact ctgtagaaca
      181 ctctactact ccgctccgtg aatcctctta ctaatggcta ccgccactgg cgatttttcg
      241 tgtctgttcc gggttggact caagatgata gttaccggga caacgtggta gtcgggctga
      301 acggggggtt cgtgcataca gtccagcttg gagcgaactg ccttcccgga accgagtgtc
      361 aggcgtggaa tgagataaac gcggccataa cagcggattg acaccggtaa accgaaaggc
      421 aggaacagga gagcgcacga gggagccacc agggggaaac gcctggtatc tttatagtcc
      481 tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg
      541 gagcctatgg aaaaacggct tcgccgtggc cttatcgctt ccctgttaag cttcttcctg
      601 gcatctccca ggaaatttcc gccccgcctg taagccggta ccgctcgccg cagccgaacg
      661 accgagcgca gcgagtcagt gagcgaggaa gcggaatata tcttgtatca catattctgc
      721 tgacgctccg ctgctgcatt tttctcctgc cacatgaagc atttcactga cactcgcatc
      781 cgtgccaaca tagtaagcca gtatacactc cgctagcgct acgtgactgg ttcagggctg
      841 cgccccgaaa cccgccaaaa gcctgacgcg cctgcggctt gtccaacccc gcgccgaccg
      901 ggaaaggctt tcccgcccgt cccggcgtta tcaggaggcc ggtttgtcag agaaacggaa
      961 caagatgctc accatgtggg tgacggagga cgagcaccgg cggctgcttg agcgctgcga
     1021 cggcaggcag ctggcagcgt ggatgcggca gacctgcctg gacgaaaagc cggcacgttc
     1081 cggaaagctt ccctcaatct cgccggcgct gctgcgccag cttgccggca tgggcaataa
     1141 cctgaaccag atagcccgcc gtgttaacgc cggcggcggt accggccatg acagggtgca
     1201 gattgttgcg gcgctgatgg ccatcgatgc cggacttgag cggctgcggc acgccgttct
     1261 tgagaaagga acggacgatg atcgttaaat ttcatccacg cggtcgcggc ggtggcgccg
     1321 ggccggtgga ctatctgctg ggtaaagacc ggcagcgtga aggcgccacc gtcctgcagg
     1381 ggaagccgga agaagtccgg gagctgattg acgcgtcgcc ttacgttaag aaatacacct
     1441 ccggggtact gtcgtttgcg gaagcggatt taccctccgg ccagcgggaa aagctgatgg
     1501 caagctttga gcgggttctg atgcccggac ttgataaaga ccagtacagc atcctgtggg
     1561 tggagcatcg tgacaagggg cggcttgagc tgaattttct gatcccgaac acggagctgc
     1621 tgaccggcag acggctccag ccgtattacg atcgcgccga ccgtccacgc atcgatgcct
     1681 ggcagaccat cgtgaacggc agactggggc tgcacgaccc gaacgcaccg gagaaccggc
     1741 gggcactggt cacaccgtct gcactgccta aagcgaagca ggaggctgca gaagcgatta
     1801 cacgcggtct gcttgctctt gcctcatccg gggagctgaa aacccgtcag gatgtcactg
     1861 aggcgctgga aagcgcaggt tttgaggtgg tccgcaccac aaaaagcagt atcagcattg
     1921 ccgacccgga cggggggcga aacatccgac tgaagggagc catttatgaa cagtctttta
     1981 acgctggcga aggacttaga gcagaaatcg aaagcgcagc agcagagtac cggcgagatg
     2041 ctgaaagccg cattcagcga gcacgagaaa tctgtaaaag cggaactgaa cgaaagcgcg
     2101 aagagaatca gcgccgccat cctcgaccac gaccggaaac tgtcctcagc catgagccag
     2161 cgcacgaaag gcatggtgcg catggtcagc cagacgtggc tgaccatcgt cctggtctcc
     2221 gcgctgctga tcgcctcgag cgcgggcatt ctgtggtggc aggggcagca gatactcgac
     2281 aactacacga ccatccggga gcagaagagc acgcaggcca tgctgtcaga gaggaacagc
     2341 ggcgtgcagc tctcgacctg cggcgagcag agacgccgct gcgtgaaggt gaacccggaa
     2401 gcgggacggt tcggagagga ttcgagctgg atgatactgg cggggaaata gcacatgacg
     2461 gagctggaaa aacagttgct gagcgcatta gagcagctac agcaggacta ctcgaaaagg
     2521 ctggacgagt gggagagcgc cttcgcggaa tggcggacga tgtctggtct tatgcaacgg
     2581 gagaacgcag cgctgagcga gcgcgtcacg ggcttgagca ggcaggtgca gagtttgagc
     2641 gagcagctgc gccggttgtc gagcgactga atgccgtcga gctgcaccgg gagcaggagc
     2701 gcgtggtgca gcatcagaaa gcgctggagc tggagcgatc tcagcgacag cggacgtata
     2761 acagcccttc actgtgataa cctgataaaa cggggctttc ggtggacatc tgcgctgaaa
     2821 gccggagcca ttcacccgac tgcatagcca tgcagccggg tgaaatttat ctggcgcaga
     2881 atgcgcggtt tagcggagag tttgctgccg gttttaccgg gctgacgccg catttgttcc
     2941 gaacccgtcc ggggcggtgc tgataaggga tgttatggta aatgcggcat tttttattat
     3001 atggagtgta tttttatgag aattaaaaag cctcaccttc gtccgatagt cataaccttg
     3061 tccgttggtg ggattttgtt aacttctatt tttctgattg ccgttattat ggtttttcag
     3121 cgcgaaaata tagaaaaaga tttgcttgat gctaattcat cgtacgctat gaaaatgtca
     3181 gatgtaatga gtggttatat tgaaatggct caaggtgaat tatcatatgg agcaaaaaaa
     3241 ataaaatcaa ctaatgatat tgactcactt aaagatgagg cggataggtt aagattgcag
     3301 tctggtatgt ttaattcagt ttttgttgtt agtaatgatg ctgttgttct ggcgacttcg
     3361 ccagaatctc ttgatttagt tggtacccgc ttgaattcaa gtgccagcaa attggcgatt
     3421 gaggaaaaga aaccattcat atcttcaccg ttcaaatcag ttactgggaa tttgattatt
     3481 ttgttgtcac atcctattta tgataagaat ggcaattaca tagggtatgt cggtgggtct
     3541 atatatctaa aaaaacatag tttatttagc gatgttttga gcaggcattt tttcaagagc
     3601 gacactgaaa ttagcattgt tagtgatgat ggaactgtga tctttaataa cgataacagt
     3661 gctgttggtc ggcccatggt tatgtctgat gatttgaaat caaagttaac ggagtctgat
     3721 agaggtgagg gggaattttt tttttcgggg aatagatatc ttctgggtta tgctcatatg
     3781 aaaaatactg cttggaatgt gttggtttat agccatgctg acagcgcgac tactattctt
     3841 attacttatg ctaaaagtgt acttgttctg ctaattgtaa taattattac attttcactt
     3901 ctttcatatt tcattgcctc ccgtatttca cttcctttag aaaagcttgc tatatctacc
     3961 aatgctaaag atataggggc ttcgttagtt tatattaaag ctatcaatgc ttggtatgcc
     4021 gaagctgatc gacttaaaaa ggcacttttt acgcatgtta aggtaatgat ggaaagggtt
     4081 gattctttaa atgaggtggc gcttaaagat ccccttaccg gcgtgaacaa tagattgggg
     4141 ttttcaacta aaatacagaa taacaagtat ggggcaggag cttcagttat tgctattgat
     4201 gttgattttt ttaaaaaaat aaatgatagt ttcgggcatg gggtcggaga tgaggtgctt
     4261 gtttctttgg ctcagattat taagtcttgt tgccgtagtg aagatattat ttgtcggttt
     4321 ggtggagaag agtttgttgt ttttctgccg aatacatctg tggtaacagc ggagtgtgtt
     4381 gcggagcgta ttaggagtgt tattgaacgt actgttttcc ctaataactt gcgtgtcacc
     4441 gtatctgctg gtgtggcaac acaagatgat cctcttggtg ggatcgattt tttgctgaaa
     4501 aatgctgacg atgctcttta tcaagctaag agagaggggc gtaataaagt gattgtatat
     4561 agcgctattt gagttttttt gatgacgctt tacgcgctag tgtgccgttt ttttttgtgg
     4621 ggaggggggt tcaagaagcg gatcctcttg ttatcccttc gatgtcg
//
