LOCUS       NZ_CP019842            11748 bp    DNA     circular CON 11-APR-2024
DEFINITION  Enterobacter roggenkampii strain R11 plasmid pASM3, complete
            sequence.
ACCESSION   NZ_CP019842
VERSION     NZ_CP019842.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06323809
            Assembly: GCF_002007805.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 11748)
  AUTHORS   Zhong,C., Cao,G., Zhang,C., Jiang,T., Chen,W. and Zong,G.
  TITLE     Complete Genome Sequence of Enterobacter cloacae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11748)
  AUTHORS   Zhong,C., Cao,G., Zhang,C., Jiang,T., Chen,W. and Zong,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2017) Shandong Medicinal Biotechnology Center,
            Shandong Academy of Medical Sciences, 18877 JINSHI ROAD, Jinan,
            Shandong 250062, China
COMMENT     ##Taxonomic-Update-Statistics-START##
            This Genome (query)               :: GCA_002007805.1
            Current Name                      :: Enterobacter roggenkampii
            Previous Name                     :: Enterobacter cloacae
            Date Updated                      :: 2019-03-07
            Analysis Type :: Average Nucleotide Identity (ANI)
            Analysis 1 (A1) :: Query vs. TYPE genome for current name
            A1 Genome (subject)               :: GCA_001729805.1
            A1 Name                           :: Enterobacter roggenkampii
            A1 ANI                            :: 98.6683%
            A1 Query Coverage                 :: 88.48%
            A1 Subject Coverage               :: 90.16%
            Analysis 2 (A2) :: Query vs. TYPE genome for previous name
            A2 Genome (subject)               :: GCA_000025565.1
            A2 Name                           :: Enterobacter cloacae
            A2 ANI                            :: 89.13348%
            A2 Query Coverage                 :: 79.89%
            A2 Subject Coverage               :: 71.25%
            ##Taxonomic-Update-Statistics-END##
            ##Genome-Assembly-Data-START##
            Assembly Method :: Newbler v. 2.8; other v. Jan-2017
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 181.0x
            Sequencing Technology             :: Illumina MiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002007805.1-RS_2024_04_11
            Annotation Date                   :: 04/11/2024 13:33:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,807
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,619
            CDSs (with protein)               :: 4,619
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 20 of 74
            Pseudo Genes (incomplete)         :: 58 of 74
            Pseudo Genes (internal stop)      :: 11 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            Pseudo Genes (short protein)      :: 1 of 74
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019842.1.
            Bacteria and source DNA available from Dr GC at Shandong Academy of
            Medical Sciences.
            The organism was changed from 'Enterobacter cloacae' to
            'Enterobacter roggenkampii' in March 2019.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11748
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="R11"
                     /isolation_source="sewage water"
                     /db_xref="taxon:1812935"
                     /plasmid="pASM3"
                     /geo_loc_name="China:Shandong"
                     /collection_date="2016-12-17"
     gene            508..831
                     /locus_tag="B1H21_RS24150"
                     /old_locus_tag="B1H21_24445"
     CDS             508..831
                     /locus_tag="B1H21_RS24150"
                     /old_locus_tag="B1H21_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_176686566.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKSARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1040..>1480
                     /locus_tag="B1H21_RS25100"
                     /old_locus_tag="B1H21_24450"
                     /pseudo=""
     CDS             1040..>1480
                     /locus_tag="B1H21_RS25100"
                     /old_locus_tag="B1H21_24450"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1509..2006
                     /locus_tag="B1H21_RS24160"
                     /old_locus_tag="B1H21_24455"
     CDS             1509..2006
                     /locus_tag="B1H21_RS24160"
                     /old_locus_tag="B1H21_24455"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_063930303.1"
                     /translation="MSNLFTASRDFEEKLKAQQQSTGEMLKAAFSEHEQSVGAELSASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTTLRQQEDALAKMSAKTWGVSYQSDSNGRFLVLPKGAKAETGWTVDSGKRNAVKLV
                     QE"
                     /name="MbeB family mobilization protein"
     gene            2013..2228
                     /locus_tag="B1H21_RS24165"
                     /old_locus_tag="B1H21_24460"
     CDS             2013..2228
                     /locus_tag="B1H21_RS24165"
                     /old_locus_tag="B1H21_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2833..3273)
                     /locus_tag="B1H21_RS24170"
                     /old_locus_tag="B1H21_24465"
     CDS             complement(2833..3273)
                     /locus_tag="B1H21_RS24170"
                     /old_locus_tag="B1H21_24465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045337632.1"
                     /translation="MAWHTVQDALKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRVYGELRTHETPKRHNKGHPENGSIHEENPQLEAIQQKLDALQKTITMMLEDKIARE
                     TEKRVQEEERNQLQAEISRLTDALEQEKKRGFWLRLFRSGSE"
                     /name="hypothetical protein"
     gene            3590..5878
                     /locus_tag="B1H21_RS25160"
                     /old_locus_tag="B1H21_24470"
     CDS             3590..5878
                     /locus_tag="B1H21_RS25160"
                     /old_locus_tag="B1H21_24470"
                     /inference="COORDINATES: protein motif:HMM:NF018642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="WP_053089601.1"
                     /translation="MSGNGDDNAHNSAFGGGKNPGMGNTGSGSNSGSSGSGNTGSHFNG
                     PAVTYGPDGSPRINLSDASSSQPGGSGGNNGGDRGGNSGSQSGNNNSSDPSKGNGTGGV
                     PTTWDRTANYYSSSDQVSRKALKNMYEEAVRTGNIPAAARGSLRANVQAMLDEDKRLAD
                     ARAAAEAEQRRLAAETERQRQAYDAAVKADAGAMAQAAAALKSADDARLAAENALQVAR
                     SRQASIGNLPGTWDRTANYYSSSDQVSRKALKNMYEKAVSTGSIPAAARGNLREKVQAM
                     LDEDKRLANARAAADAEVQRLTIALSNAQQAVVAAQKQQADTIAQAAEKARQRQAEEAE
                     RQRQAAAAEQKRLADIAAEQARIAAEQEKVRQARQAAVNKLKSSGIQSVRGLPTTAAPA
                     ASPLTWSVASAGGISLGSDVAGAVWSRISAALAELRGIMAASMAGPVAVTVAGLLYSRD
                     VGVGSDVVPGRDISAMMPGDAFSLPDTATLDAAADGKTGVSMPVRGRMVLRDDGTLETQ
                     LVRTTVAGNVPVVRGVLDPATGYWSYALPAMAGVPGQTILVSPSDAPGINGPLGLAGPV
                     PLPEFIVHTGGEDSAPQGVTVTTTPVTEDLDFNDLILIFPSDSGLKPLYIMLRNPRNIP
                     GTASGNGQQTGDNWLGSAGTGDGAPIPSQIADKLRGREFGSFDAFRRAVWGEVGKEATL
                     TSQFNESNKKALRKGYSPFPPEAEQVGGRTRYELHHITPIKDGGAVYNIDNIRILTPKR
                     HIDLHKNGE"
                     /name="S-type pyocin domain-containing protein"
     gene            5880..6137
                     /locus_tag="B1H21_RS24180"
                     /old_locus_tag="B1H21_24475"
     CDS             5880..6137
                     /locus_tag="B1H21_RS24180"
                     /old_locus_tag="B1H21_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003082343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_045337636.1"
                     /translation="MDKKNITEYTEAEFLAFVKKICDADYDTENKHTEAVLLFKKLTEH
                     PDGSDLIYYPKPGHDDSPEGIVKTVKEWRAANGKPGFKSE"
                     /name="bacteriocin immunity protein"
     gene            6205..6360
                     /locus_tag="B1H21_RS24185"
                     /old_locus_tag="B1H21_24480"
     CDS             6205..6360
                     /locus_tag="B1H21_RS24185"
                     /old_locus_tag="B1H21_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_049224158.1"
                     /translation="MKRVLFLDVIVVSMMLAACQVNNVRDTGGGAVSPSSTVTGVTLGS
                     ETDGNP"
                     /name="colicin release lysis protein"
     gene            7321..7644
                     /locus_tag="B1H21_RS24195"
                     /old_locus_tag="B1H21_24495"
     CDS             7321..7644
                     /locus_tag="B1H21_RS24195"
                     /old_locus_tag="B1H21_24495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_021243036.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7853..>8287
                     /locus_tag="B1H21_RS25110"
                     /pseudo=""
     CDS             7853..>8287
                     /locus_tag="B1H21_RS25110"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8322..8813
                     /locus_tag="B1H21_RS24850"
     CDS             8322..8813
                     /locus_tag="B1H21_RS24850"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_047720453.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            8820..9035
                     /locus_tag="B1H21_RS24855"
     CDS             8820..9035
                     /locus_tag="B1H21_RS24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            9963..11249
                     /locus_tag="B1H21_RS24205"
                     /old_locus_tag="B1H21_24505"
     CDS             9963..11249
                     /locus_tag="B1H21_RS24205"
                     /old_locus_tag="B1H21_24505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899670.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_223230178.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWERLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTRKGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     Typing          223..395
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.276"
                     /coverage="100.0"
                     /evalue="9.21e-81"
     Typing          7035..7207
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.571"
                     /coverage="100.0"
                     /evalue="4.3800000000000006e-64"
     Typing          complement(6532..7110)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.859"
                     /coverage="99.0"
                     /evalue="1.5100000000000002e-163"
     Typing          complement(11291..11748)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.656"
                     /coverage="99.0"
                     /evalue="4.900000000000001e-133"
     Typing          822..2320
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.283"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7322..7638
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="100.0"
                     /evalue="9.11e-61"
     Typing          7635..9133
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.099"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt
       61 tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gcttcgccgt ggccttatcg
      121 cttccctgtt aagcttcttt ctggcatctc ccaggaaatt tccgccccgc ctgtaagccg
      181 gtaccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaat
      241 atatcctgta tcacatattc tgctgacgct ccgctgctgc atttttctcc tgccagatga
      301 agcacttcac tgatattcgc atccgtgcca atatagtaag ccagtataca ctccgctagc
      361 gctacgtgac tggttcaggg ctgcgccccg aaacccgcca aaagcctgac gcgcctgcgg
      421 cttgtccaac cccgcgccga ccgggaaagg ctttcccgcc cgtcccggcg ttatcaggag
      481 gccggtttgt cagagaaacg gaacaagatg ctcaccatgt gggtgacgga ggacgagcac
      541 cggcggctgc ttgagcgctg tgacggcagg cagctggcgg cgtggatgcg gcagacctgc
      601 ctggacgaaa agtctgcgcg caccggaaag ctcccctcaa tctcgccggc gctgctgcgc
      661 cagcttgccg gcatggggaa taacctgaac cagatagccc gccgtgttaa cgccggcggc
      721 ggcaccggcc atgacagggt gcagattgtg gcggcgctga tggccatcga tgccgggctt
      781 gagcggctgc ggcacgccgt tcttgagaaa ggaacggacg atgatcgtta aatttcatcc
      841 acgtggccgc ggcggtggcg ccgggccggt ggactatctg ctgggtaaag accggcagcg
      901 tgaaggcgcc agcgtcctgc aggggaagcc ggaagaagtc cgggagctga ttgacgcgtc
      961 gccttacgtg aagaaataca cctccggcgt cctgtcgttt gcggaggccg atctgcctcc
     1021 cggccagcgg gaaaaactga tggccagctt cgagcgggtt ctgatgcccg gactcgataa
     1081 agatcagtac agcattctgt gggtggagca cgcggacaag gggcgtctgg agctgaactt
     1141 cctgatcccc aataccgagc tgctgaccgg cagacggctc cagccatact acgaccgagc
     1201 cgaccggccg cgcatcgatg cctggcagac catcgtgaac ggcagactgg ggctgcacga
     1261 cccgaacgcg ccggaaaacc gccgggcgct ggtcacgccg tccggcctgc cgaaaacgaa
     1321 gcaggaggcc gcagaagcca ttacccgggg gctgctgagc ttagcctcgt caggggagct
     1381 gaaaagccgt caggacgtca ctgacactct tgaaggtgca ggttttgagg tggtgcgcac
     1441 cacgaaaagc agtatcagca tcgccgaccc ggacgggggg cgaaacatcc gactgaaggg
     1501 agccatctat gagcaatctt tttacggcct cgagggactt cgaggagaaa ttgaaagcgc
     1561 agcagcagag taccggcgag atgctgaagg ccgcattcag cgagcacgag cagtctgtcg
     1621 gagcggaact gagcgcaagc gcgaggagaa tcagcgacgc catcagcgcc cacgagcaga
     1681 gtatgagcga ggcgatggag aaaaaccggc ggagcgtgct gctgacggca gggaggacat
     1741 ggcttaccat cctgatggtt tcagcgctgc tgatcggcac gagcgggagc attctgtggt
     1801 ggcaggggca gcagataacc gacaattaca cgacccttcg ccagcaggag gacgccctgg
     1861 cgaagatgag cgcgaaaaca tggggtgtga gctaccagag cgacagcaac ggccggttcc
     1921 tggtgctgcc gaagggagcg aaagccgaaa caggctggac agtggacagc gggaagcgga
     1981 acgcggtgaa gctggtacag gagtagagca gaatgacaga gctggaaaag cagttgctga
     2041 gcgcattaga gcagctacag caggactact cgcaaaggct ggacgaatgg gagagcgcct
     2101 tcgcggaatg gcggagcatg tgtgggctta tgcaacggga gaacgcggtg ctgaacgagc
     2161 gcgtgtcgga cttgagcacg caggtgctga gtttaagcga gcagctgcgc cgcttgtccg
     2221 ggaactgaat gccatcgagc tccggcagag ggagaatgag tatcagaaaa cactggagct
     2281 ggagcgccag ccgcagaaaa cctaccgcgg gccgacactt taaaccatcc aggcagctga
     2341 caacccgcac gaagaggggc tcttatttct cccgctaaat aggttcgtga tcaaaaaggt
     2401 cacgacttat tcaggagcgg gatatgaagc tgttaaatct tattgaatca ttgcttaact
     2461 gcctgtatct ggcggcggag ctgtgggact ggctggagaa gcatttgtcg gtttttaagg
     2521 cattggcgcg gattttagtg tgactttaac atccatgcat ctggctgcat gactatgcac
     2581 cagtgtaata ttcagcggct gcgtttctgg tgttttttcg ttatgtctgt cgccactttc
     2641 tcgcgtctgg caccggaaac gcagccaacc ccgtttcagc ggtgcgccca acgggtgtta
     2701 tggtaaatca ggtcagcttt ctttgggggg ctgcggtttt tttgggagtc cccctgcatt
     2761 ctttacacca gctttgtatg accatctttt ccgcctaaac cttccgataa cggatgttag
     2821 tcaccggtat ggttactctg aacctgagcg aaacagccgc aaccagaaac cccgcttttt
     2881 ttcctgctcc agcgcgtccg tcaaccgact gatttccgcc tgcagttgat tgcgctcctc
     2941 ttcctgcact cgtttttctg tttcccgcgc gattttatcc tcgagcatca tggtgatggt
     3001 tttctgaagt gcatccagct tctgctgaat ggcctctaac tgcggatttt cctcgtggat
     3061 actaccattt tcagggtgtc ccttattgtg cctttttggt gtctcatgag tgcgcagctc
     3121 accatagact cgaatcagtt cactggtctc gaactcccgg cgatcatttt tccctgtgcg
     3181 atagctgaca aggcctttag ccatgtcacg atataattgc gaacgcgatt ttcctgttaa
     3241 tttcagtgcg tcctgaactg tgtgccatgc cataaaagtg acagtgtctc ctgtgtgtcc
     3301 cgtatgccag tgtgtgagca tacatccggt tttaacacgg aatgtcatag gttgtcagtc
     3361 ggtggtgaaa aatgggaaag aaaaccttgt tgacgatatg actttttcgg gtgtagcatc
     3421 aaacaactgt atataaacac atgtttttat gtacagtatt tgtggtgtgg cagagtactt
     3481 ataacagcct gcagggaacc cgcccggtgg cttccggcag gcaataaacc gcacacctaa
     3541 tttctcagaa gaacaagtca aacaaaaaaa ataagaagag gatgaagtca tgagtggtaa
     3601 cggtgacgat aacgcccaca acagcgcatt cggtggcggg aagaatcccg gcatggggaa
     3661 tacaggctca ggcagtaatt caggatcatc cggcagcgga aatacaggca gtcattttaa
     3721 tggcccggca gtaacatacg gtccggacgg gtctccgcgt attaacctgt ctgatgccag
     3781 ctcttcacag cccggtggca gcggtggtaa taacggcggt gatcgcggtg gaaattcagg
     3841 ctctcagtcg ggtaacaata attcttctga tccgtcgaaa ggaaatggca ccggcggggt
     3901 acccacgaca tgggaccgta cggcaaacta ttacagcagc agcgatcagg tctcacgtaa
     3961 ggcgcttaaa aatatgtacg aggaagcggt caggacggga aatatcccag cagccgcccg
     4021 tggtagtctc cgtgcaaacg tgcaggcaat gctggatgaa gacaaacgtc tggcggatgc
     4081 ccgggccgct gcagaagccg agcagcgtcg tctggctgct gaaactgagc gtcagcggca
     4141 ggcttatgac gctgcggtta aagctgatgc cggtgccatg gctcaggcag cggcagcgct
     4201 aaaatcagca gatgatgctc gactggcagc cgaaaacgca cttcaggtag cgcgttcccg
     4261 tcaggcttcg attggtaatc tacccggtac gtgggaccgt acagctaatt attacagcag
     4321 cagcgatcag gtctcacgca aggcacttaa aaacatgtat gaaaaagcgg tcagtacggg
     4381 gagtattcct gctgccgccc gcggtaatct tcgtgaaaaa gtgcaggcga tgctggatga
     4441 agacaaacgt ctggctaatg cccgcgcggc agctgatgct gaagttcaac gtctgaccat
     4501 cgctctgtcg aacgcccagc aggcagttgt cgccgcccag aaacagcagg cggatactat
     4561 cgctcaggct gcggaaaaag cgcgtcagcg tcaggccgaa gaggcggaac gtcagcggca
     4621 ggctgcggcc gcagagcaaa agcgcctggc agacattgcg gctgaacagg cccggattgc
     4681 agctgaacag gaaaaagtcc gtcaggccag acaggctgcg gtgaataaac tgaaatccag
     4741 cggtatccag tccgtgcgcg ggctgccgac gacggcggcg ccggcagcct ctccactaac
     4801 ctggtccgta gcctctgccg gtggcatttc tctaggcagt gatgtggcag gtgcggtctg
     4861 gtcacgtatc tctgcggcac ttgctgaact gcgtggcatt atggccgcca gcatggccgg
     4921 gccggtggcg gtaaccgttg ctggcctgct gtattcacgt gatgttggtg tgggaagtga
     4981 cgtcgttccc ggccgtgata tcagcgcgat gatgccgggg gatgcgtttt cactgcctga
     5041 tactgcgacg ctggacgcgg cggctgacgg aaaaaccggt gtcagcatgc cggtgcgtgg
     5101 tcgtatggta ttgcgtgatg atggcaccct ggagacgcag ttggtcagaa cgactgttgc
     5161 cggtaatgtt ccggtggtgc ggggcgtgct ggatcctgcc acaggctact ggagttatgc
     5221 gttgccggcg atggctggtg ttcccggtca gaccattctg gtcagcccgt cggatgcgcc
     5281 cggtatcaat ggtccactgg gactggcggg gcctgttccg ctgccggaat tcattgtgca
     5341 taccggtggt gaggattccg caccgcaggg agtgactgtt acaacgaccc cggtgacgga
     5401 agatctggat tttaacgacc ttattctgat attcccgtca gactccggcc tgaagccact
     5461 gtacatcatg ctgcgtaacc cgcgtaacat tccgggtacc gcgagtggta acggtcagca
     5521 aactggtgat aactggctgg gtagtgctgg tactggtgat ggtgctccta ttccgagtca
     5581 gattgcagat aagctgcgcg ggcgtgagtt cgggagtttt gatgcgttcc gccgtgccgt
     5641 atggggtgag gttggaaaag aagcaacgtt gactagtcag ttcaatgaaa gtaacaagaa
     5701 agcccttcgt aaaggatatt ctcctttccc tcctgaagct gagcaggttg gtgggcgcac
     5761 tcgttacgaa ctccaccaca tcacgcctat taaagatggt ggggccgttt ataatattga
     5821 caatattaga attttaacgc caaaaaggca catcgatctt cataagaatg gtgaatgata
     5881 tggacaaaaa gaacattact gaatataccg aagctgaatt tttggctttt gttaagaaaa
     5941 tttgtgatgc agattatgac accgaaaata agcatacaga agctgtcctg ttgtttaaaa
     6001 aattaactga acaccctgac ggttctgatc tcatctatta tccaaaaccc ggtcatgacg
     6061 acagccctga aggtattgtt aaaacagtaa aagagtggcg agccgcaaat ggtaagcctg
     6121 gctttaagtc agagtaatga ttttcaggag ccttcattga aggctccttt catccggata
     6181 attataaaaa taaaaggact gaatatgaaa agagtacttt tcttagatgt aatagtggtt
     6241 tcgatgatgc tggcagcatg ccaggttaat aatgtccgcg ataccggtgg cggggcagtc
     6301 tcaccgtcat caacagtgac tggtgtcacg ctcggcagtg aaacagatgg caatccataa
     6361 aggatcttct tgagatcctt ttcaatgagt cgtaatctct tgctctgtaa acgaaaaaac
     6421 cgcctgggga ggcggttttt cgaaggttaa gtcagttggg gaactgctta acccggtaac
     6481 agggttcacg agaccgccat taccaaaact gttcttccag tgtagcctct gttaggccac
     6541 tacttcaaga cctcttatct atcttctcgt gtactcctgt taccagtggc tgctgccagt
     6601 ggcgttttgt cgtgtctttc cgggttggac tcaagacgat agttaccgga taaggcgcag
     6661 cggtcgggct gaacgggggg ttcttgcaca cagcccagct tggagcgaac tgtctaaacg
     6721 gaacgggacg tggtgattta ggtaaagctt ccaccacaac acggatgccg catgacggga
     6781 acaggaaagc gcaagaggga gccaccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6841 gggtttcgcc accactgatg tgagcgtctg atttcgtgat gcttgtcagg ggggcggagc
     6901 ctatggaaaa acggcaacgc cgtggcctta tcgtttcctg ctaagttctt tcctggcatc
     6961 tactaggaaa tttccgcccc gcctgtaagt tggtaccgct cgccgcagcc gaacgaccga
     7021 gcgcagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg
     7081 ctccgctgct gcatttttct cctgccacat gaagcacttt cctgaacccc caccccgtcg
     7141 taacatacta agccagtata cactccgcta gcgctacgtg actggttcag ggctgcgccc
     7201 cgaaacccgc taaaagccac tgacgcgcct gcggcttgtc caaccccggc cgaccgggaa
     7261 cagatttccc gcccgtcccg gcgttaagag gagaccggtt tgtcagagaa acggaacaag
     7321 atgctcacca tgtgggtgac ggaggacgag caccggcggc tgcttgagcg ctgcgacggc
     7381 aggcagctgg cggcgtggat gcggcagacc tgcctggacg aaaagcctgc gcgtaccgga
     7441 aagcttccct ctatctcgcc ggcgctgctg cgccagcttg ctggcatggg caacaacctg
     7501 aaccagatag cccgccgcgt taacgccggc ggcggtaccg gtcatgacag ggtgcagatt
     7561 gtggcggcgc tgatggccat cgatgccgga cttgagcggc tgcggcacgc cgttcttgag
     7621 aaaggaacgg acgatgatcg ttaaatttca tcctcggggt cgcggcggtg gcgccggtcc
     7681 agtggactat ctgctgggta aagaccggca gcgtgaaggc gccagcgtcc tgcaggggaa
     7741 gccggaagag gtccgggagc tgattgacgc gtcgccttac gtgaagaaat acacctccgg
     7801 cgtcctgtcg tttgcggagg ccgatctgcc tcccggccag cgggaaaaac tgatggccag
     7861 cttcgagcgg gttctgatgc ccggacttga taaagatcag tacagcattc tgtgggtgga
     7921 gcacgaggac aaggggcgtc tggagctgaa ctttctgatc cccaataccg agctgctgac
     7981 cggcaggcgg ctccagccgt actacgaccg cgccgaccgg ccgcgtatcg atgcctggca
     8041 gaccatcgtg aacggcagac tggggctgca cgatccgaac gcgccggaaa accgccgggc
     8101 gctggtcacg ccggccggac tgccgaaaac gaagcaggaa gccgcagaag ccatcacccg
     8161 tggactgctg agcttagcct cgtccgggga gctgaaaagc cgtcaggacg tcactgaggc
     8221 tcttgaaggt gcaggttttg aggtggtgtg tacaacgaaa agcagtatca gcattgccga
     8281 ccctgacggg gggcgaaaca tccgactgaa gggagccatc tatgagcagt cttttaacgc
     8341 tggcgaagga cttagagcag caatcgaaag cgcagcagaa gagtaccggc gagatgctga
     8401 aagccgcatt cagcgagcac gagcagtctg tcagagcgga actgaacgca agcgcgagga
     8461 gaatcagcga cgccatcagc gcccacgagc agagtatgag cgaggcgatg gagaaaaacc
     8521 ggcggagcgt gttgctgacg gccgggagga catggctcac catcctgatg gtgtcagcgc
     8581 tgctgatcgc cacgagcggg agcattctgt ggtggcaggg gcagcagata accgacaatt
     8641 acacgcatct tcgtcagcag gaagataccc tggcgaaaat gaccgcccgg acgtggggag
     8701 tgcgctatca ggagagcagc gacggccgga ggtttcttat cctgccgccg gggatgcaag
     8761 ccgaagccat cccctacgac gggacgacat ggatccgcct gaaacaggag tagcggaaaa
     8821 tgacagagct ggaaaagcag ttgctgagcg cattagagca gctacagcag gactactcgc
     8881 aaaggctgga cgaatgggag agcgccttcg cggaatggcg gagcatgtgt ggtcttatgc
     8941 aacgggagaa cgcggtgctg aacgagcgcg tgtcggactt gagcacgcag gtgctgagtt
     9001 taagcgagca gctgcgccgc ttgtccggga actgaatgcc atcgaggaga aggctgcact
     9061 ggagtatcag cgtcagaacg agctggagct gacgctccga cagaagcaga agagtttcga
     9121 tggtccgaag atgaggtgac agcccgcacg aagaagggct catcgcgttc ctgctaaata
     9181 ggctgactac cggaatggta gtgacttatc ccggaggaag tgatgaaaat tctcaacgtt
     9241 attgaagccc tgctgaactg tttttacctg gtgatggagc tgtgtgagtt gccggagagg
     9301 catttgccgg tttttaaggc gttcgcgctg attttagtgt gactttacgc tgcatgcatc
     9361 tggctgcatg actatgcatc agtgtaatat ccagcggctg cgtttttggt gttttttcgt
     9421 catgtctgtc gccactttct cgcgtctggc accggaaacg cagccaaccc cgtttctgcg
     9481 gtgcgggcaa ggggtgttat gttaaaagcc gaggtctggc ggatacggaa aaaattatgc
     9541 cggccgggct tatgcccgcg gaaatccggc cgatacttaa attatccttc acaaaggata
     9601 tcctgcgatc cagtcagttg agtgttttcc gtctcccgga tgggagccat ttttttgcct
     9661 tctgatgcgt gttaaggctt gcggagctgc gctggttctt ccaagattga gagacgccag
     9721 cttcctgctg gagccggcgg atttgccgta aatatgaaca ttttcttatt gttcagcccg
     9781 ctaaagatgg cgggtttttt acgcgtgaaa tccggagccg gacatcatgc cggcatggct
     9841 gaagagcggg ctttttcccg ccctgtcgca gtaatatata aagctacata aggagtagcc
     9901 gataaccatg gcagtactta cattatccgg gcaatctgat tcatatgttt tcacttaaat
     9961 atatgttctc cctgaacctc cagcgatcag ccatttcgta tgacgcaatt tgtcagaata
    10021 agcctgtcat tgagttcagt ccggaggggg tgataaataa agccagcccg ctttttctct
    10081 ccactatggg gtacagggct gatgaaataa tcggtcacca ccatcgtata ttctgcccgc
    10141 cctcgctggt aagttcaccg gaatacagcc agttctggca gcgccttgcc aggggagaaa
    10201 gctttagtgg caagtacctg cggctggcaa agggaaatcg ttccgtatgg cttgaagcca
    10261 gttatatccc cgtttctgac aggcgcggcc gtgttatcag agtcattaaa attgctgccg
    10321 atatttctga gcgcgtgcat tctgctcttg agcaggaagc agtcgtaaat gccataaacc
    10381 gttctatggc catcatcacc ttcaatcctg aagggatcgt gcttgaagca aatgaaaatt
    10441 tcgtcaatgc cactggctat aagcgggatg aaatcatagg taagcatcat cgtctgttct
    10501 gtgccgagac gctttacaaa agtgatgaat accgacattt ctgggagaga ctgaatcaag
    10561 gtgagttttt ttctggtctg tttccacgcc ttaaccgtca gggagaccct ctgtggttcc
    10621 gtgcaactta taaccctgtc tttaacagtg atgggcagct ttataaaatt gtgaaattcg
    10681 ctacagatgt tacggctgat gtcctgcgaa accagagaga gcaagaggct gctgtgcatg
    10741 catgggatat ggccgtgcag acccgagaaa gtgcgcagaa cggtgccaat gttattgaaa
    10801 atagcatcct tatgattgac agaattgcgc aggggatggg ggctgtctcc accgatatct
    10861 cacggctcaa caatcagtct gaaagtattg acgatatggt ggaaaccatc cggaagtttg
    10921 ccatgcagac aagacttatt gcactgaatg ccgcgattga agcagcaagg gctggcgcat
    10981 ccggaagaag ttttgcggtt gttgccgcag aagtgcgtaa tcttgcggcg agcgtcagta
    11041 gcgcgactga agaaattgag caagtcgtgg ccagcaatag tcaactggcc aaggatgttc
    11101 tctgtggcat tgaaaacagc ctgatgaaca ctcggaaagg ggttacactc atgcgcgaag
    11161 cgggtgaagt tatgaccagc attcagagga atgctgcagg ggttgagact gcggttaagg
    11221 atgtcgccat ttcagttaaa gccggatagg ccatggtgca tatccccata tctgtcgaac
    11281 atcacagatg atcttcttga gatcctttcc gttgcgtcgt aatctcttgc tctgaaaacg
    11341 aaaaaaccac ccggcaaggt ggctttttcg aaggttcgca agagttgacg ctcttttgaa
    11401 ccgcggtaac tggcttggag gagcacagtc accaaaactg tcctttcagt gtagccttat
    11461 cgcacccgaa acttcaagac tctcctcctc taaacctgtt accagtggct gctgccagtg
    11521 gcgctttgtc gtgtttttcc ggattggact caagttgata gttaccggat aaggcgcagc
    11581 ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcccgg
    11641 aactgagtgt caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta
    11701 aaccgaaagg caggaacagg agagcgcacg agggagccac caggggga
//
