LOCUS       NZ_CP019567             4817 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Staphylococcus aureus strain SR434 plasmid pSR04, complete sequence.
ACCESSION   NZ_CP019567
VERSION     NZ_CP019567.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06277866
            Assembly: GCF_001986135.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Chen,Y.
  TITLE     Complete Genome Sequence of a Community-Associated
            Methicillin-Resistant Staphylococcus aureus Strain of Sequence type
            88 from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Chen,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2017) Infectious Diseases, Zhejiang University, 3
            East Qingchun Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: DEC-2016
            Assembly Method                   :: HGAP v. Dec-2016
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 01:39:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,860
            CDSs (total)                      :: 2,778
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 39 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 15 of 79
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019567.1.
            Bacteria and source DNA available from Yunsong Yu.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="SR434"
                     /isolation_source="skin"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pSR04"
                     /geo_loc_name="China: Hangzhou"
                     /lat_lon="30.26 N 120.20 E"
                     /collection_date="2015-11-26"
                     /collected_by="Yan Chen"
     gene            13..498
                     /gene="lnu(A)"
                     /locus_tag="BZP34_RS14850"
                     /old_locus_tag="BZP34_14850"
     ARG             14..495
                     /name="lnu(A)"
                     /gene="lnu(A)"
                     /gene_name="lincosamide nucleotidyltransferase Lnu(A)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1234..2169)
                     /locus_tag="BZP34_RS14855"
                     /old_locus_tag="BZP34_14855"
     CDS             complement(1234..2169)
                     /locus_tag="BZP34_RS14855"
                     /old_locus_tag="BZP34_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440612.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_060554450.1"
                     /translation="MSRENYSKYLQNKDSDILHSTGYSNSRLGVHSVHPQNPKLSFDAM
                     TIVGNLSRDNAQKLSSFMSIEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWDRRN
                     MRVEFNPNKLTHEEMLWLKQNIINYMEDDGFTRIDLAFDFEDDLSNYYAMTDKAVKKTV
                     FYGRDGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVMSDHLWRVEIELKRDMVDYW
                     NDCFNDLHILKPDWTSPEKLNEQAMVYMLIHEEGKWGELNKRTKYKYKNLIKEISPVDL
                     TELMKSTLKENEKQLQKQIEFWQTKRKKLF"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(3607..4611)
                     /locus_tag="BZP34_RS14865"
                     /old_locus_tag="BZP34_14865"
     CDS             complement(3607..4611)
                     /locus_tag="BZP34_RS14865"
                     /old_locus_tag="BZP34_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020368559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_077156147.1"
                     /translation="MQYNTTKYIDENQDNETLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNVLEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQRVI
                     EEVVKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTESFRRLFKYKKVSKNLIGFMRST
                     EVTVNKNDGSYNQHMHVLLSVENSYFKNKANYITQEEWVSLWQKALQVDYRPVANIKAI
                     KPNQKGDKDIQAAIKETSKYSVKSSDFLTDDDERNQEIVNDLEKGLYQKRMLSYGGLLK
                     QKHKILNLDDAEDGNLINTSDEDKTTDEEEKAHSITAIWNFEKQNYYLKNLKR"
                     /name="protein rep"
     ARG             14..498
                     /name="lnuA"
                     /gene="lnuA"
                     /gene_name="lincosamide nucleotidyltransferase (LNU)"
                     /tool="rgi"
                     /drug_class="lincosamide antibiotic"
                     /identity="97.52"
                     /coverage="100.0"
     Typing          complement(1260..2082)
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.083"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(3608..4611)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.109"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aggagggata aaatgaaaaa taataatgta acagaaaaag atttatttta tattttagat
       61 ttatttgaac acatgaaagt aacttattgg ttagatggtg gctggggggt agatgtatta
      121 actggaaaac aacaaagaga acacagagat atagatatag attttgacgc tcaacacact
      181 caaaaagtta tacaaaaatt agaagatatc ggatacaaaa tagaagttga ttggatgcct
      241 tcacgcatgg aacttaagca tgaagaatat gggtatttag atattcatcc tataaatcta
      301 aatgatgatg gatcaattac ccaagcaaac ccagaaggtg gtaattatgt tttccaaaat
      361 gactggtttt cagaaactaa ttacaaaggt cgaaaaatac catgtatttc aaaagaagct
      421 caacttcttt ttcattctgg ttatgattta acagaaaaag accattttga tataaaaaat
      481 ttaaaatcaa taacataatt gctttattcc aattgcttta ttgacgttga gcctcggaac
      541 ccttaacaat ccctaaactt gtcgaatcgt cggcttaata gctcacgcta tgccgacatt
      601 cgtctccaag tttagttaag ggttcttctc aacatcaata aattttctcg gcataaatgc
      661 gtgtgatacg attaatgtat tactcctttt catttaatat atcaaaaact attccctggc
      721 tactcaaaaa atgagtagtc tttttatttt gcattttaag ccctttctag gacatttaat
      781 cattccacgt ataaaaataa ctaaaaactt ttttcgttca acagagaagc tataaatcat
      841 tttaaaattc attttctaaa tcttttagaa tacgaaatat gtttttctat ctcggttatt
      901 cttcttaata aaaagctaaa gccttaaaaa ataggtgtat aatgtaactc ttgtatttta
      961 aagagcataa gaggaatcct gcacagaaat gtctccgtat gtatccattc ggtagccatt
     1021 tctgtgcaaa atattgattt gacagtgtca aatgggattc ctatttatgc gataaaaatt
     1081 gttatatgac tcccgaacgg ctacacaaaa gataccgaat agctacacaa aattaaaaat
     1141 ggctcccaaa tggctacata aaaatggata ccaaatggct ccatttttat gtagccattt
     1201 gggagctaaa agggagccga atggtagcca tttttaaaat agcttcttcc tttttgtctg
     1261 ccaaaattct atttgctttt gtaattgttt ttcattttct tttaaagtcg atttcattaa
     1321 ttccgttaaa tcaactggag atatttcttt aattaaattt ttatatttat atttagttct
     1381 tttattcaat tctccccatt tgccttcttc atgaattaac atgtaaacca ttgcttgttc
     1441 atttaatttt tctggcgaag tccaatctgg tttcaagata tgtaaatcat taaaacaatc
     1501 attccagtaa tccaccatat ctcttttaag ttcaatttct acacgccata agtgatcaga
     1561 cataacttcg acatctgcgt tatctttacg ttcttgtttt ttgttataaa ttctaataaa
     1621 tctatcacta tcacgaacgc caaaatattt tgtttctggt ttcccgtcac gaccataaaa
     1681 tacagttttc ttaactgctt tatcagtcat tgcataataa ttacttaaat catcttcaaa
     1741 atcaaaagct aaatctattc ttgtaaaacc gtcatcttcc atgtagttga taatgttttg
     1801 ttttaaccaa agcatttctt catgcgtaag tttattcgga ttaaattcaa cacgcatatt
     1861 acgtctatcc catgtatctg cttttacttt gtcgtattca atataaactt tttcttgtag
     1921 tgctttagct ttaaatttcg tttgtagtat atcccaaagt cgaatttgtg gttcgatact
     1981 cataaatgac gatagctttt gagcgttgtc acgactgaga tttccaacta ttgtcatagc
     2041 gtcaaaactt aatttaggat tttgtgggtg cacagaatgc actcctaacc ggctattaga
     2101 gtagccggtt gagtgtaaaa tgtcactatc cttattttgt aagtactttg aataattttc
     2161 tctactcata aaaaatcgac tccttaattt gttttaagaa gtcgctcacc cgaatatata
     2221 tcttgaagaa aaaactaata tcgtttaata tctagatata caaaatataa aaaacaactt
     2281 aacgtttttc cttaggtaag tcgcattcac aaactttggt cggggagtga gcgacttctt
     2341 ttttattttg ttattaatat aacactatca aaaagcttgg tctattatga ccaagctttt
     2401 tggtgtttat aaacctatgt caaaatactt atattatcaa gacaagaaga aactcgtttc
     2461 aaaaaagcca aaaaatctca tcgttaaata cttttgaatg cactatgatt aaataaatgt
     2521 taaagtgaaa atataattct caaacatcat taattctttt aacatgaaat agttcttcta
     2581 ccaacccata gtcactcaat aattgagtga ctatttttaa tggttttgga tgagatgaaa
     2641 ataaataagt gttaaagtaa aaatatagca ttatcctcat tcacatttag tcttttaatg
     2701 tgaagtgatc ttcgcccaaa aatagctact cacaaattga gtggctattt tttaatgctt
     2761 tattccaatt gctttattga cgttgagcct cggaaccctt aacaatccca aaacttgtcg
     2821 aatggtcggc ttaatagctc acgctatgcc gacattcgtc tccaagttta gttaagggtt
     2881 cttctcaaca tcaataaatt ttctcggcat aaatgcgtgt tctatttttt attatcgtct
     2941 tgataattat aaaaataaaa cactcctttt tttagcaaac tttatcacaa tagttatcgc
     3001 tttacagatc tatttttcat cgttataatc tagttaattc ccgtaagggc gcacttatgc
     3061 gttctaaaag aacgcgtgta aaaaccataa tcgaccttcg tcgatttttt tatttcaaaa
     3121 ttaaagaaac ggcttgagat attccgagca gttataaaaa ttaatttgtc ttgaatattg
     3181 ctactacatt cttgaaaaag tgatgtagat ttatagccca taactttttt gaattccatc
     3241 gacgatacgg cgtcgaaaga aaaataaaca gaccgtacga aagaaaaaag ctatctcaat
     3301 tttggggtag cttttatttt ttatgccttt attttgaatt ttaagggggc attttaaaga
     3361 attaggggta aatcatatag ttttatacca aaaaacttat ataccctctt aaaacgcaaa
     3421 tatgagccaa ataaatatat cttaatttca caaactaaaa ttcgagcaaa tccagtgttg
     3481 attttttaag acactgccca tttacatgca aacttaaaag ttggcatgaa aagtggtcaa
     3541 ccatgccggt tgaacgctat agttcccgca ggggcaaaaa tacataaaaa aacgctagct
     3601 tttaagctaa cgtttcaaat tttttaaata ataattttgt ttttcaaaat tccaaattgc
     3661 cgtaatcgaa tgtgcttttt cttcttcgtc tgttgtttta tcttcgtcac ttgtattaat
     3721 caaattacca tcttcggcat catctaaatt taaaatctta tgtttttgtt taagtaatcc
     3781 accataactc aacatacgct tttggtataa acctttttct aaatcattca cgatttcttg
     3841 atttctttca tcatcatcag ttaaaaaatc agatgactta accgaatatt tagaggtttc
     3901 tttaatagct gcttgaatat ctttatcgcc tttttgattt ggtttgatcg ctttaatatt
     3961 tgctacgggt cgataatcaa cttgtaatgc tttttgccat aaactaaccc attcttcttg
     4021 agttatataa ttagctttat tcttaaaata actattttca acacttaaca aaacatgcat
     4081 atgttgatta taactgccgt catttttatt aactgttact tctgttgaac gcataaaacc
     4141 aattaaattt ttactaactt ttttatattt aaataatctt ctaaatgatt cagttaagtg
     4201 tttcaaactt tgttctaaag tatccccatc tatagcattt tttgttgaaa gtgttagaaa
     4261 taaccaacgt gctttcggtt tttccttaac cacttcttca atcactcttt gagcttgata
     4321 actatttttc atagcacgcc tccaattaca aactggacat aattttgatt tacaaaacca
     4381 tgtcttatgt aacttcaaat aaccttcatc agtcggcttg aactctaaga cgttaccaca
     4441 ttgttttacg ttaaaagcct ttttaatttt taaaatttcc agtatgtctg cataacttac
     4501 attatcaatt ttcttttctc tccatgggcg ttgcttccca cttttcgtca tatcttttaa
     4561 tgtttcgtta tcttgatttt cgtcaatata tttagtagta ttatattgca tataaaaagc
     4621 ccccagtttt atttaagatt cgacacctta ataatataac tggggttttt tcatgtcaaa
     4681 ttttcttttc aactccaaaa atacataaaa acactgtcat atcaacgaca aatcatgttt
     4741 ttaaaaaaga acgttatttc tatatagtat caagacaaga agaaactcgt tttcaactcg
     4801 tttcaaattc ccaagtt
//
