LOCUS       NZ_CP019278            11397 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Escherichia coli strain 13P477T plasmid p13P477T-5, complete
            sequence.
ACCESSION   NZ_CP019278
VERSION     NZ_CP019278.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06219553
            Assembly: GCF_002951655.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11397)
  AUTHORS   Ho,P.L., Lau,A., Liu,C.J., Lai,E.L.Y., Chow,K.H., Tse,C.W.S. and
            Que,T.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2017) Microbiology, The University of Hong Kong,
            102 Pok Fu Lam Road, Hong Kong, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 30-NOV-2016
            Assembly Method                   :: HGAP v. NOV-2016
            Assembly Name                     :: 13P477T
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 04:11:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,487
            CDSs (total)                      :: 5,362
            Genes (coding)                    :: 5,063
            CDSs (with protein)               :: 5,063
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 299
            CDSs (without protein)            :: 299
            Pseudo Genes (ambiguous residues) :: 0 of 299
            Pseudo Genes (frameshifted)       :: 156 of 299
            Pseudo Genes (incomplete)         :: 162 of 299
            Pseudo Genes (internal stop)      :: 54 of 299
            Pseudo Genes (multiple problems)  :: 69 of 299
            Pseudo Genes (short protein)      :: 4 of 299
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019278.1.
            Bacteria and source DNA available from Ho Pak-Leung, the University
            of Hong Kong.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="13P477T"
                     /isolation_source="stool"
                     /host="pig"
                     /db_xref="taxon:562"
                     /plasmid="p13P477T-5"
                     /geo_loc_name="Hong Kong"
                     /collection_date="2013-04"
     gene            128..493
                     /locus_tag="BWI89_RS29465"
     CDS             128..493
                     /locus_tag="BWI89_RS29465"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_236919639.1"
                     /translation="MQLNRKKTVVIQPLPENLNSHFLPSLTENSVSICFVSFVLHWLSV
                     TEWQWMPLSMLRVKRVTSVIIMLMFLCSTRRLSEEGFKDKTRELDDFKSGEVNYWRSAW
                     SEMTNRHLEKSWPFRAY"
                     /name="MobA/MobL family protein"
     gene            1310..1744
                     /locus_tag="BWI89_RS28040"
                     /old_locus_tag="BWI89_28020"
     CDS             1310..1744
                     /locus_tag="BWI89_RS28040"
                     /old_locus_tag="BWI89_28020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_148119650.1"
                     /translation="MRKSGAYEMSTPGERLADQLAAKENPILAGQIKSLNAQKNAEDMA
                     AYRTRVQSRKQEKADMKLETIEELKQRGDARRDKADKAERAELEKASHQASLQNETIGE
                     ETKELESGEKEKLSKRDQVLEKFKAKFERDKQNERGGRGR"
                     /name="hypothetical protein"
     gene            1917..2306
                     /locus_tag="BWI89_RS28045"
                     /old_locus_tag="BWI89_28025"
     CDS             1917..2306
                     /locus_tag="BWI89_RS28045"
                     /old_locus_tag="BWI89_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618753.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_236919638.1"
                     /translation="MKAEASLRLEGIDPSGIPRYESIKARVISGEITYEQGRAEILAFH
                     TGKCAEPTTAGEMLAEEFLKPLNMSQGELAQATGIPEHHIDDIISNRRRLSAEEPYKLA
                     EQFQTDKDFWLNLQALHDAWLTHQK"
                     /name="HigA family addiction module antitoxin"
     gene            complement(2466..2819)
                     /locus_tag="BWI89_RS30295"
     CDS             complement(2466..2819)
                     /locus_tag="BWI89_RS30295"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227664254.1"
                     /translation="MITQLKGRKIALVSFCWYELICTQMNGNQFSQEKKVKGSKPSIWS
                     ITEGEVIMATINLSLLEHDCGEAAREALLSGVPVYYADETTPENCVIKEYPDGRRELVS
                     FMTGMEKLVGANT"
                     /name="hypothetical protein"
     gene            complement(3208..3348)
                     /locus_tag="BWI89_RS29470"
     CDS             complement(3208..3348)
                     /locus_tag="BWI89_RS29470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071994383.1"
                     /translation="MQEPPVQPDCCAFPGNYRLESNPGRHDKAPLAAAIGNWKFAENLF
                     S"
                     /name="replication initiation protein"
     gene            3707..4069
                     /locus_tag="BWI89_RS28060"
                     /old_locus_tag="BWI89_28040"
     CDS             3707..4069
                     /locus_tag="BWI89_RS28060"
                     /old_locus_tag="BWI89_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_113398075.1"
                     /translation="MAWHSVSQAQNMTGKSRRTLYRDMASGRLSWRTNASGHRELETSE
                     LIRVYGEIKKDGTAERHIVAQPDDTVPSRVMMDELRALRQEVSDLKSMLLRLEHKPEKP
                     PEVAAPWWRFWQKKST"
                     /name="entry exclusion protein 1"
     gene            complement(4322..6805)
                     /locus_tag="BWI89_RS30300"
                     /old_locus_tag="BWI89_28050"
     CDS             complement(4322..6805)
                     /locus_tag="BWI89_RS30300"
                     /old_locus_tag="BWI89_28050"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_025769090.1"
                     /translation="MKKNIQAGIFFVTMLLPVWHAAAETFTRCHPNETVWAMIQDEQLK
                     NIARLQETKRCQAVNDTYDQWRQNFQAMGDAENAIRSARDNQNWNAVKSTFDNIAPQIN
                     SLHALAEKNSSLLGAGNILALFGGDLNYLYQNAGLVAPASADGLTDNVSQMLTEPDKQV
                     KAAGIAQQLVYQTRVRAQSFIQGVAQNAGNEAQKEYREQSDKIVEDKAHNFKGDSVEGY
                     FGGFLARISQVYAPVLLFLIIVGIIGALTARRVRLDAFTASVKAVMAAVVPAVALALML
                     IFMPFLPSWVLYTAALAGTFAIYSRSKQILGGLASRCGRFPTLASGLRWMGGFVSGIDA
                     DRKQETGAASVNPASHHTHGSSRWGTAAEMRENNHLLPTEKQAGFTLARELSPPPGVDG
                     KFCFTGHVVTVAPTGSGKGIGAVIPNLLTYQGSALVFDPKGENAEVTGRRRREMGQSVH
                     VIDPFGITSGIKESVNILDRLDVTSPDCVAESAVLAESLVVSEKGGDSNHFDESARTLL
                     QGLMLHVSGLPDESRRNLAELRRLITSDEENLLALLADMAASEDVAFGIPARAANTLMG
                     MADRERGSVLSTARKNTAFLDDPRIAAALSHSDFNLSAIKAEPMTVYLVLPANRIAANT
                     RFIRLFIGSVISAVTESKAKPAHNVAFILDEFNVLGYMKSIEDAVSLLRGYGLSFWVFL
                     QDLSQLKSAYPKWQTFLANSAKTFYGTDDYDTAKYVSDTLGKSTVEFETQNSGRNSGSG
                     VSGGGGSMNRGRSSGSSQQFAGRELLTPDEVMRLGPTRPIVILKGEYPYQLTRINYLTD
                     PEYAGMADPNPYYSA"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     gene            complement(6853..7155)
                     /locus_tag="BWI89_RS28075"
                     /old_locus_tag="BWI89_28055"
     CDS             complement(6853..7155)
                     /locus_tag="BWI89_RS28075"
                     /old_locus_tag="BWI89_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023139325.1"
                     /translation="MAKSRAEKLEELRQKEAQIKAQIQQLKQREQTEERKKDTRRKILI
                     GGAVLAKIKRGEWNYHQLVSLLDNELKNDRDRALFDEMGLKAVDARKESFNPGNK"
                     /name="hypothetical protein"
     gene            7315..9138
                     /gene="mobQ"
                     /locus_tag="BWI89_RS28080"
                     /old_locus_tag="BWI89_28060"
     CDS             7315..9138
                     /gene="mobQ"
                     /locus_tag="BWI89_RS28080"
                     /old_locus_tag="BWI89_28060"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_023139326.1"
                     /translation="MAIFHLSVKTISRSAGRSATAAIAYRTGSRIVDERTGITHDYTRK
                     SGVEHSEIFLPQNSPGWASNRETLWNAVEQKENRRNSTVAREFELAFPAELDGKQRLDL
                     LREFCSSLVERHGMAVDAAIHAPGKEGDQRNYHAHVLCSTRRLSEEGFKDKTRELDDFK
                     SGEVNYWRSAWSEMTNRHLEKAGRSERIDHRSLEAQRESAISQGDSAKAAELDRVPTIH
                     IGYAAAQMEKRGIETERGNEHRQIILRNAEIQPLSRAIQLIENWRATHDNNSRAGHGSI
                     APVPARDTGRDRRGVRTAQSNTGRAGAVPPPSARGRLRNLSELDMVRFGERSEMLLPGN
                     VSRDVEHQRTDRNQPLRRENTVTSKPKRPEIIRLEWQRLRRDKLTFVHDKAGRIAARGA
                     AQVERQRQKISSHERQRPEQPSGLIGMFKRSAYEQAASTWQTIKNGLDRRLVQLKNRLG
                     FVRGYMRKSGAYEMSTPGERLADQLAAKENPILAGQIKSLNAQKNAEDMAAYRTRVQSR
                     KQEKADMKLETIEELKQRGDARRDKADKAERAELEKASHQASLQNETIGEETKELESGE
                     KEKLSKRDQVLEKFKAKFERDKQNERGGRGR"
                     /name="mobQ"
     gene            9311..9700
                     /locus_tag="BWI89_RS28085"
                     /old_locus_tag="BWI89_28065"
     CDS             9311..9700
                     /locus_tag="BWI89_RS28085"
                     /old_locus_tag="BWI89_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618753.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_236919638.1"
                     /translation="MKAEASLRLEGIDPSGIPRYESIKARVISGEITYEQGRAEILAFH
                     TGKCAEPTTAGEMLAEEFLKPLNMSQGELAQATGIPEHHIDDIISNRRRLSAEEPYKLA
                     EQFQTDKDFWLNLQALHDAWLTHQK"
                     /name="HigA family addiction module antitoxin"
     gene            complement(9861..10214)
                     /locus_tag="BWI89_RS30305"
     CDS             complement(9861..10214)
                     /locus_tag="BWI89_RS30305"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227664254.1"
                     /translation="MITQLKGRKIALVSFCWYELICTQMNGNQFSQEKKVKGSKPSIWS
                     ITEGEVIMATINLSLLEHDCGEAAREALLSGVPVYYADETTPENCVIKEYPDGRRELVS
                     FMTGMEKLVGANT"
                     /name="hypothetical protein"
     gene            complement(10604..10744)
                     /locus_tag="BWI89_RS29475"
     CDS             complement(10604..10744)
                     /locus_tag="BWI89_RS29475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071994383.1"
                     /translation="MQEPPVQPDCCAFPGNYRLESNPGRHDKAPLAAAIGNWKFAENLF
                     S"
                     /name="replication initiation protein"
     Typing          complement(3240..3607)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.566"
                     /coverage="85.0"
                     /evalue="8.49e-135"
     Typing          complement(10636..11003)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.566"
                     /coverage="85.0"
                     /evalue="8.49e-135"
ORIGIN
        1 ttcaagaatt gttgatgagc gaactgggat taccacgact acacccgtaa atcaggcgtc
       61 gagcattcag agatttttct ccctcaaaac tcaccaggtt gggcgagtaa tcgggagact
      121 ctctggaatg cagttgaaca gaaagaaaac cgtcgtaatt caaccgttgc cagagaattt
      181 gaactcgcat ttcctgccga gcttgacgga aaacagcgtc tcgatctgct tcgtgagttt
      241 tgttcttcac tggttgagcg tcacggaatg gcagtggatg ccgctatcca tgctccgggt
      301 aaagagggtg accagcgtaa ttatcatgct catgttttta tgcagtaccc gccgactgtc
      361 ggaggaaggt ttcaaggata agacccgtga acttgatgat tttaagagcg gcgaagtcaa
      421 ctactggcgt tctgcatggt cagaaatgac gaaccgtcat ctggaaaaaa gctggccgtt
      481 cagagcgtat tgaccatcgt tcacttgagg cgcaacggga atcagctatt tctcaggggg
      541 attccgccaa ggcagcggag ctggatcggg ttccgactat tcatatcggt ttacgcagct
      601 gcgcagatgg aaaagcgcgg catagagacc gaaccgaggc aatgaacatc gccagataat
      661 attgcgtaat gctgaaatcc agccgctttc acgggcaatt cagctgattg aaaactggaa
      721 gggcaaccca tgacaacaac agcagagcag gacatggaag cattgctcca gtccctgcac
      781 gagacacagg gcgagatagg agaggagtcc gcaccgctca gtccaacact ggccgcgctg
      841 gagccgtccc gccgccagcg caaggggtcg cctgcgcaac ctgtccgaac tcgattatgg
      901 ttcgcttcgg agaacgaagt gagatgttat tgcgggcaat gtatctcgtg acgtggagca
      961 ccagagaacc gacagaaatc agcctctgcg acgggaaaac actgtaacca gcaagccaaa
     1021 gcgaccggaa attatccggc ttgaatggca acgtttacgc cgggataagc tgactttcgt
     1081 tcacgataag gccggacgga ttgccgcccg tggggcggca caggttgaac gccagagaca
     1141 aaaaatatcc tcacatgagc gacaaagacc agaacagcca gcggcctgat aggaatgttt
     1201 aagcgcagcg cctatgaaca ggccgccagt acctggcaga ccatcaaaaa tggtctcgat
     1261 cgtcgtctgg tgcaactgaa aaatcggctt ggctttgtca gaggctacaa tgcgcaagtc
     1321 tggcgcttac gaaatgtcta cgccgggaga acggctggcc gaccagctgg ccgcgaaaga
     1381 aaatccgatc ctggctgggc agataaaatc gctcaatgct cagaagaacg cggaagatat
     1441 ggccgcatac cgtacccgcg tacagtcccg caaacaggag aaagctgata tgaagctgga
     1501 aactatcgaa gaactcaaac agcgtggtga tgcccggaga gataaagccg acaaagccga
     1561 acgtgcagag ctggaaaaag cgtctcatca ggcttcactg cagaatgaaa cgatcggcga
     1621 agagacaaaa gaactggagt ccggagagaa agagaaactc agcaagcgtg atcaggttct
     1681 ggagaagttt aaagcaaaat tcgagaggga taagcagaat gaacgtggtg gccgtgggcg
     1741 ttagcggtat gcaacacagc gccaaccatg ctgcatacct gacccggcca gactgatcag
     1801 gcgtcttttc gggctgaaag cattgtactg gttaacagta tttcatgaca tatgaggtac
     1861 gcctgatatt gacatatgag gtagatatga gtgaagaaca gcgcaggcgt ggcttcatga
     1921 aggctgaggc aagcctgaga ctggaaggta tagatcccag cggaatcccc cgctacgagt
     1981 cgattaaagc gagagttatt tcgggtgaga taacctatga gcagggacga gctgaaattc
     2041 tggctttcca taccgggaag tgtgcggagc cgacaactgc gggggaaatg ctcgcagagg
     2101 aatttttaaa acctctgaac atgtctcagg gggaactggc gcaggctaca ggaattcctg
     2161 agcatcatat cgatgatatt atcagtaacc gccgtcgcct cagtgctgag gagccttaca
     2221 agctggccga acagttccag accgataaag atttctggct caacctgcag gctttgcatg
     2281 acgcatggct gacgcaccag aaatagcagc caggggcgcg attagatata taatgttcca
     2341 gtctaatgta ggatgttcca tgcaggtcag agtattaaaa ataaagcccc gtccttacca
     2401 ggcggggctt tattttttcc tgcccctcaa gtgtcataat ggttctgaaa tccggacatc
     2461 caaaatcatg tattagcccc aacgagtttc tccataccag tcatgaagct caccagttcc
     2521 ctgcggccat ccggatattc tttgataacg cagttttctg gtgtcgtctc atcggcgtaa
     2581 tagacaggaa cgccgctgag aagagcttcg cgggcggctt cgccgcagtc atgttccagt
     2641 agtgatagat taatggtcgc catgatgact tctccttcgg taatactcca aatggatggt
     2701 tttgaccctt tgaccttctt ttcctggcta aattgattgc cattcatttg tgtgcatatc
     2761 aattcatacc aacaaaaact caccagtgcg attttacggc cttttaattg ggtaatcatg
     2821 gtcttttgag gcttagggca taaaaacgcg ctgagggcct ctgaggcggt tctgctaacg
     2881 gatgttatgg taaatgcgag tgaccggcat ggtgactctt aatttatttt tcgacttctc
     2941 ccccccctcc ttgcccctac aaccccatgg agcccatgcc acttgtcaac ttcacgggtt
     3001 tgtcatgatc ttcttgagat tctttgttta tcgtcgtagt ctcttgtcct gtaaacgaaa
     3061 aaaccacccg gccaggtggt ttttcgaagg ttcagtaaac cggcaagttt ctgaaccgtg
     3121 gtaactggct tacgcagagc acagcaacca aatctgtcct tccagtgtag ccgtactttg
     3181 gcgcacactt caagagcaac cgcgtgttta gctaaacaaa ttctctgcga acttccagtt
     3241 accaatggct gctgccagtg gcgctttgtc gtgtcttccc gggttggact caagacgata
     3301 gttaccggga aaggcgcagc agtcgggctg aacggggggt tcttgcatac agcccagctt
     3361 ggagcgaacg acctacatcg agctgagata cctacagtgt gagctatgag aaagcgccac
     3421 atgtcccgta agggaaaaag gcggtcaggt atccggtaaa gcgacacggt cggaacagga
     3481 gagcgcaaga gggagcgatt caccggaaac ggtgatgatc tttatagtcc tgtcaggttt
     3541 cgccgcctct ggcttgagcg tctgtttttg tgatgctcgc caggggggcg gagcctatgg
     3601 aaaaacgggt ctgccgcttt tttttcccct tcttgccgcg tgtcaggatg ccgttagcct
     3661 gctagagtgc agcccgtaga gtgtgtcact taatcaaggg ggtgttatgg catggcacag
     3721 tgtgtcacaa gcacaaaaca tgacagggaa aagtcgcaga accctttatc gggatatggc
     3781 gtctggccgc ttgtcatggc gaactaatgc tagcggccat cgcgaacttg agacctcaga
     3841 gctgatccgt gtttatggcg agattaaaaa agatggcaca gctgaacggc acatcgtggc
     3901 acagcctgat gacaccgtgc ccagtagggt catgatggat gaattgcgtg ctttacggca
     3961 ggaagtttct gaccttaaat ctatgctgtt aaggcttgag cataaaccgg aaaagccgcc
     4021 tgaagtagcg gctccgtggt ggcgtttctg gcagaaaaaa tctacttaac tcttttctct
     4081 ttttcgcggc taaagttgat tacgttatcc ggtagagttt caggctttgt ggtcttttgt
     4141 gtctctattg tcatagcact acgtcttctg ttttgcgtca ccagagccca gataaaggca
     4201 cagataaagc ggaaagagaa gtatatcggt atgcctatca caaaggcgac ggcggttaag
     4261 gccgggagca agacccggcc aaacatcaga gctaacagta cgccgacaac gattccaaat
     4321 atcatgcgct gtagtagggg ttaggatctg ccataccggc gtattctggg tctgtcagat
     4381 agttaatacg ggtgagctga taagggtatt cccctttcag aatcacaatt ggtcgggttg
     4441 gtccaagcct catgacttcg tcaggtgtca gtaattccct cccagcaaac tgctgacttg
     4501 atccagacga acgaccccga ttcatggagc cgccaccccc ggaaacaccg ctgccagagt
     4561 tgcggcctga attctgggtt tcaaattcaa ccgtcgattt acctagggtg tcgctgacgt
     4621 attttgcagt gtcatagtcg tctgtgccgt agaacgtctt cgctgagttg gccagaaacg
     4681 tctgccattt agggtaagca cttttcagct gtgacagatc ctgcaggaat acccagaaag
     4741 acagcccgta tccgcgtaac aggcttacgg catcctcgat tgatttcata taccccagca
     4801 cgttgaactc atcgaggatg aatgcgacat tatgagctgg cttcgccttc gattctgtta
     4861 cagcggagat cacagaaccg ataaacagcc ttataaatcg ggtatttgct gcaatgcgat
     4921 tagctggtag cacgagataa actgtcatcg gttcagcttt gatggctgac aggttgaagt
     4981 ctgagtgtga cagcgcagct gctatacgcg ggtcgtccag aaatgctgtg tttttacggg
     5041 cggtagacaa tactgagcct cgttcccggt ctgccatccc catgagcgtg ttggccgctc
     5101 gggcggggat accgaaagca acgtcttcac ttgcggccat atcggccagc agggctaaca
     5161 ggttttcttc atcactggtg atcagtcgac gtagttccgc gagattacgt cgggactcat
     5221 ccggtaatcc ggaaacatgc agcatcaggc cctgtaacag ggtccgggct gattcatcaa
     5281 aatgattact gtcaccacct ttctctgata ccacaaggct ttcggccaga actgcagatt
     5341 cagcgacgca atccgggctg gtcacatcga gacgatccag aatgttcact gattctttta
     5401 tgccgctggt tatgccgaat gggtcaatta cgtggacgct ctgacccatt tcacgacggc
     5461 ggcgacctgt cacctcggcg ttttcccctt ttggatcaaa gacaagcgct gaaccttgat
     5521 aagtcagaag attgggaatt actgcgccaa tcccttttcc gctgcccgta ggggcaacag
     5581 tcacaacgtg cccggtgaaa cagaattttc catccactcc gggcggtggg gacagttctc
     5641 gtgccagggt gaatccggct tgcttttccg ttggtaacag gtggttattt tcccgcattt
     5701 ctgcagcggt tccccagcgt gatgagccgt gagtgtgatg tgaggccggg ttgacggatg
     5761 ccgcgcccgt ttcctgcttt ctgtcagcat cgatgccaga cacaaaaccg cccatccagc
     5821 gaagcccgga ggccagtgtg gggaaacgcc cacagcgaga ggccagccct ccaagaattt
     5881 gtttactgcg ggaataaatg gcgaatgttc cggccagcgc tgccgtgtac aatacccatg
     5941 atggtaggaa aggcatgaat atcagcatca gcgccagtgc tactgcaggt accactgcgg
     6001 ccataactgc tttcactgat gccgtgaaag catccagcct tacccgccgt gcggtaagtg
     6061 caccaataat accaacgatg ataaggaaca gcagaactgg agcatatacc tggcttatcc
     6121 gggcaagaaa gccgccgaaa tatccttcca cgctgtcacc tttaaaatta tgggctttat
     6181 cctcgacaat cttatctgat tgctcacgat actctttctg ggcttcgtta ccggcgttct
     6241 gtgccacgcc ttggataaaa ctctgtgccc gaacccgagt ctgataaacc agctgttgcg
     6301 cgatacctgc agctttgacc tgtttatccg gttcagtcag catttgactg acgttatctg
     6361 tcagtccgtc agcgctggcc ggggcaacga gccctgcatt ctggtacagg taattcagat
     6421 cgccgccgaa aagcgccagg atgttacccg cgcccaataa cgatgaattt ttttctgcca
     6481 gtgcatgcag gctattaatc tggggagcaa tgttatcgaa cgtggatttt acggcgttcc
     6541 agttctgatt atcgcgggcg ctgcggatgg cgttttctgc atctcccatc gcctggaaat
     6601 tttggcgcca ctggtcataa gtgtcattaa cggcctgaca ccgctttgtt tcctgtagtc
     6661 tggcgatgtt ttttagctgc tcatcctgga tcattgccca gacagtctca tttggatggc
     6721 agcgggtgaa tgtttcggcc gctgcatgcc atactggcaa tagcattgtg acgaagaaaa
     6781 tcccggcctg tatatttttt ttcatgctaa atctcctgat ctgattcatc atccgagaat
     6841 aagtaattgg cgtcacttat taccggggtt aaaagattct tttctggcgt caaccgcctt
     6901 taaacccatt tcatcaaata gtgcccggtc tctgtcattt ttcagctcat tgtccagaag
     6961 actcaccagc tgatgataat tccattcccc acgctttatt tttgcgagta cggcaccacc
     7021 gattaggatt tttcgtcggg tatccttctt gcgttcttct gtctgctctc tttgctttaa
     7081 ttgctgaatt tgcgccttta tctgagcttc tttctgacgc aactcttcca acttttccgc
     7141 tcttgatttt gccattattc acctctttga ttcaaatttg aacgataact ccattgctga
     7201 tatatttcaa cccgaagcga aagcaaaggg cgcacttatg caaactcttc gagtttgcgt
     7261 gcggcagggt taaaaccctg cacccaaggt caaaggaaaa acaggaatac taaattggca
     7321 atttttcatc ttagcgttaa gacaataagc cgtagtgctg gccgttcggc cactgcggca
     7381 attgcttatc gtacaggttc aagaattgtt gatgagcgaa ctgggattac ccacgactac
     7441 acccgtaaat caggcgtcga gcattcagag atttttctcc ctcaaaactc accaggttgg
     7501 gcgagtaatc gggagactct ctggaatgca gttgaacaga aagaaaaccg tcgtaattca
     7561 accgttgcca gagaatttga actcgcattt cctgccgagc ttgacggaaa acagcgtctc
     7621 gatctgcttc gtgagttttg ttcttcactg gttgagcgtc acggaatggc agtggatgcc
     7681 gctatccatg ctccgggtaa agagggtgac cagcgtaatt atcatgctca tgttttatgc
     7741 agtacccgcc gactgtcgga ggaaggtttc aaggataaga cccgtgaact tgatgatttt
     7801 aagagcggcg aagtcaacta ctggcgttct gcatggtcag aaatgacgaa ccgtcatctg
     7861 gaaaaagctg gccgttcaga gcgtattgac catcgttcac ttgaggcgca acgggaatca
     7921 gctatttctc agggggattc cgccaaggca gcggagctgg atcgggttcc gactattcat
     7981 atcggttacg cagctgcgca gatggaaaag cgcggcatag agaccgaacg aggcaatgaa
     8041 catcgccaga taatattgcg taatgctgaa atccagccgc tttcacgggc aattcagctg
     8101 attgaaaact ggagggcaac ccatgacaac aacagcagag caggacatgg aagcattgct
     8161 ccagtccctg cacgagacac agggcgagat aggagaggag tccgcaccgc tcagtccaac
     8221 actggccgcg ctggagccgt cccgccgccc agcgcaaggg gtcgcctgcg caacctgtcc
     8281 gaactcgata tggttcgctt cggagaacga agtgagatgt tattgccggg caatgtatct
     8341 cgtgacgtgg agcaccagag aaccgacaga aatcagcctc tgcgacggga aaacactgta
     8401 accagcaagc caaagcgacc ggaaattatc cggcttgaat ggcaacgttt acgccgggat
     8461 aagctgactt tcgttcacga taaggccgga cggattgccg cccgtggggc ggcacaggtt
     8521 gaacgccaga gacaaaaaat atcctcacat gagcgacaaa gaccagaaca gcccagcggc
     8581 ctgataggaa tgtttaagcg cagcgcctat gaacaggccg ccagtacctg gcagaccatc
     8641 aaaaatggtc tcgatcgtcg tctggtgcaa ctgaaaaatc ggcttggctt tgtcagaggc
     8701 tacatgcgca agtctggcgc ttacgaaatg tctacgccgg gagaacggct ggccgaccag
     8761 ctggccgcga aagaaaatcc gatcctggct gggcagataa aatcgctcaa tgctcagaag
     8821 aacgcggaag atatggccgc ataccgtacc cgcgtacagt cccgcaaaca ggagaaagct
     8881 gatatgaagc tggaaactat cgaagaactc aaacagcgtg gtgatgcccg gagagataaa
     8941 gccgacaaag ccgaacgtgc agagctggaa aaagcgtctc atcaggcttc actgcagaat
     9001 gaaacgatcg gcgaagagac aaaagaactg gagtccggag agaaagagaa actcagcaag
     9061 cgtgatcagg ttctggagaa gtttaaagca aaattcgaga gggataagca gaatgaacgt
     9121 ggtggccgtg ggcgttagcg gtatgcaaca cagcgccaac catgctgcat acctgacccg
     9181 gccagactga tcaggcgtct tttcgggctg aaagcattgt actggttaac agtatttcat
     9241 gacatatgag gtacgcctga tattgacata tgaggtagat atgagtgaag aacagcgcag
     9301 gcgtggcttc atgaaggctg aggcaagcct gagactggaa ggtatagatc ccagcggaat
     9361 cccccgctac gagtcgatta aagcgagagt tatttcgggt gagataacct atgagcaggg
     9421 acgagctgaa attctggctt tccataccgg gaagtgtgcg gagccgacaa ctgcggggga
     9481 aatgctcgca gaggaatttt taaaacctct gaacatgtct cagggggaac tggcgcaggc
     9541 tacaggaatt cctgagcatc atatcgatga tattatcagt aaccgccgtc gcctcagtgc
     9601 tgaggagcct tacaagctgg ccgaacagtt ccagaccgat aaagatttct ggctcaacct
     9661 gcaggctttg catgacgcat ggctgacgca ccagaaatag cagccagggg cgcgattaga
     9721 tatataatgt tccagtctaa tgtaggatgt tccatgcagg tcagagtatt aaaaataaag
     9781 ccccgtcctt accaggcggg gctttatttt ttcctgcccc tcaagtgtca taatggttct
     9841 gaaatccgga catccaaaaa tcatgtatta gccccaacga gtttctccat accagtcatg
     9901 aagctcacca gttccctgcg gccatccgga tattctttga taacgcagtt ttctggtgtc
     9961 gtctcatcgg cgtaatagac aggaacgccg ctgagaagag cttcgcgggc ggcttcgccg
    10021 cagtcatgtt ccagtagtga tagattaatg gtcgccatga tgacttctcc ttcggtaata
    10081 ctccaaatgg atggttttga ccctttgacc ttcttttcct ggctaaattg attgccattc
    10141 atttgtgtgc atatcaattc ataccaacaa aaactcacca gtgcgatttt acggcctttt
    10201 aattgggtaa tcatggtctt ttgaggctta gggcataaaa acgcgctgag ggcctctgag
    10261 gcggttctgc taacggatgt tatggtaaat gcgagtgacc ggcatggtga ctcttaattt
    10321 atttttcgac ttctcccccc cctccttgcc cctacaaccc catggagccc atgccacttg
    10381 tcaacttcac gggtttgtca tgatcttctt gagattcttt gtttatcgtc gtagtctctt
    10441 gtcctgtaaa cgaaaaaacc acccggccag gtggtttttt cgaaggttca gtaaaccggc
    10501 aagtttctga accgtggtaa ctggcttacg cagagcacag caaccaaatc tgtccttcca
    10561 gtgtagccgt actttggcgc acacttcaag agcaaccgcg tgtttagcta aacaaattct
    10621 ctgcgaactt ccagttacca atggctgctg ccagtggcgc tttgtcgtgt cttcccgggt
    10681 tggactcaag acgatagtta ccgggaaagg cgcagcagtc gggctgaacg gggggttctt
    10741 gcatacagcc cagcttggag cgaacgacct acatcgagct gagataccta cagtgtgagc
    10801 tatgagaaag cgccacatgt cccgtaaggg aaaaaggcgg tcaggtatcc ggtaaagcga
    10861 cacggtcgga acaggagagc gcaagaggga gcgattcacc ggaaacggtg atgatcttta
    10921 tagtcctgtc aggtttcgcc gcctctggct tgagcgtctg tttttgtgat gctcgccagg
    10981 ggggcggagc ctatggaaaa acgggtctgc cgcttttttt tccccttctt gccgcgtgtc
    11041 aggatgccgt tagcctgcta gagtgcagcc cgtagagtgt gtcacttaat caagggggtg
    11101 ttatggcatg gcacagtgtg tcacaagcac aaaacatgac agggaaaagt cgcagaaacc
    11161 ctttatcggg atatggcgtc tggccgcttg tcatggcgaa ctaatgctag cggccatcgc
    11221 gaacttgaga cctcagagct gatccgtgtt tatggcgaga ttaaaaaaga tggcacagct
    11281 gaacggcaca tcgtggcaca gcctgatgac accgtgccca gtagggtcat gatggatgaa
    11341 ttgcgtgctt tacggcagga agtttctgac cttaaatcta tgctgttaag gcttgag
//
