LOCUS       NZ_CP019263            24727 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Escherichia coli strain 13C1065T plasmid p13C1065T-4, complete
            sequence.
ACCESSION   NZ_CP019263
VERSION     NZ_CP019263.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06219550
            Assembly: GCF_002951775.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24727)
  AUTHORS   Ho,P.L., Lau,A., Liu,C.J., Lai,E.L.Y., Chow,K.H., Tse,C.W.S. and
            Que,T.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2017) Microbiology, The University of Hong Kong,
            102 Pok Fu Lam Road, Hong Kong, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 30-NOV-2016
            Assembly Method                   :: HGAP v. NOV-2016
            Assembly Name                     :: 13C1065T
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 161.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 04:40:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,348
            CDSs (total)                      :: 5,230
            Genes (coding)                    :: 4,871
            CDSs (with protein)               :: 4,871
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 359
            CDSs (without protein)            :: 359
            Pseudo Genes (ambiguous residues) :: 0 of 359
            Pseudo Genes (frameshifted)       :: 137 of 359
            Pseudo Genes (incomplete)         :: 226 of 359
            Pseudo Genes (internal stop)      :: 58 of 359
            Pseudo Genes (multiple problems)  :: 59 of 359
            Pseudo Genes (short protein)      :: 4 of 359
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019263.1.
            Bacteria and source DNA available from Ho Pak-Leung, the University
            of Hong Kong.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..24727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="13C1065T"
                     /isolation_source="stool"
                     /host="chicken"
                     /db_xref="taxon:562"
                     /plasmid="p13C1065T-4"
                     /geo_loc_name="Hong Kong"
                     /collection_date="2013-08"
     gene            complement(join(23825..24727,1..4))
                     /locus_tag="BWI86_RS29260"
                     /old_locus_tag="BWI86_27580"
                     /pseudo=""
     CDS             complement(join(23825..24727,1..4))
                     /locus_tag="BWI86_RS29260"
                     /old_locus_tag="BWI86_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185180.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /name="tyrosine-type recombinase/integrase"
     gene            complement(448..934)
                     /gene="mobI"
                     /locus_tag="BWI86_RS27380"
                     /old_locus_tag="BWI86_27405"
                     /pseudo=""
     CDS             complement(448..934)
                     /gene="mobI"
                     /locus_tag="BWI86_RS27380"
                     /old_locus_tag="BWI86_27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000045622.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein MobI(A/C)"
                     /name="mobI"
     gene            1863..2102
                     /locus_tag="BWI86_RS29725"
     CDS             1863..2102
                     /locus_tag="BWI86_RS29725"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236923347.1"
                     /translation="MTDNAFEVETRPGSFLVPRSLGKEISWDNFLWLLSMVTLTLFENM
                     TVTMLTSVFTMLGKSYFTVAGKTFIIRQNSAVTL"
                     /name="hypothetical protein"
     gene            2146..2316
                     /locus_tag="BWI86_RS29730"
     CDS             2146..2316
                     /locus_tag="BWI86_RS29730"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236923348.1"
                     /translation="MSMLAPEFVSIFFGLKPATADAIKDVGVSSLKAINDVVLFPRTIA
                     FTPGLGMIALR"
                     /name="hypothetical protein"
     gene            2483..2776
                     /locus_tag="BWI86_RS29735"
     CDS             2483..2776
                     /locus_tag="BWI86_RS29735"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236923349.1"
                     /translation="MAKVDQTKASRLLSATKTMSLGANKNSIRILYGNHVAENIDFRSE
                     PQQHVKKGIRGWSRRADNRRAFSAVIDVAVNYFGFDPIHPESSFRNIDFNAS"
                     /name="hypothetical protein"
     gene            complement(<3813..5008)
                     /locus_tag="BWI86_RS27400"
                     /old_locus_tag="BWI86_27425"
                     /pseudo=""
     CDS             complement(<3813..5008)
                     /locus_tag="BWI86_RS27400"
                     /old_locus_tag="BWI86_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424631.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /name="IS4-like element ISVsa5 family transposase"
     gene            5170..5775
                     /locus_tag="BWI86_RS27410"
                     /old_locus_tag="BWI86_27435"
     CDS             5170..5775
                     /locus_tag="BWI86_RS27410"
                     /old_locus_tag="BWI86_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001615655.1"
                     /translation="MNTYSDKIHNLIDIAKLAVAMREHSYFFALRRGIDVNFCADLNGS
                     GTQGIFIRKKSFNAYEPSFIEVIFEPTHKNDDSFLYEEDLTTDQRKDYEPSINRGKHRF
                     VAQRAKLNLDWDSNEIQQWRLDIERLSKPHNTLNDWLENDSEIMIIHLCGGYRFREPVI
                     LSQRDIKQYVASGLTLEDLKNRLKCSICGERNAKIKVF"
                     /name="hypothetical protein"
     gene            complement(5888..6202)
                     /locus_tag="BWI86_RS27415"
                     /old_locus_tag="BWI86_27440"
     CDS             complement(5888..6202)
                     /locus_tag="BWI86_RS27415"
                     /old_locus_tag="BWI86_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_077150898.1"
                     /translation="MQIKIAAPLGGDAIIEFDDNEEVSGRLSIISGDITEDMIAEAIAG
                     ANPNSYMGFVNTLDAPASDVLRTLHLYAGWFVDWPAVDGGDDDDDDDDDFGDHVDQIVY
                     "
                     /name="hypothetical protein"
     gene            6263..6955
                     /locus_tag="BWI86_RS27425"
                     /old_locus_tag="BWI86_27450"
                     /pseudo=""
     CDS             6263..6955
                     /locus_tag="BWI86_RS27425"
                     /old_locus_tag="BWI86_27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            7427..10921
                     /locus_tag="BWI86_RS30160"
                     /old_locus_tag="BWI86_27460"
                     /pseudo=""
     CDS             7427..10921
                     /locus_tag="BWI86_RS30160"
                     /old_locus_tag="BWI86_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344508.1"
                     /GO_component="GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /GO_function="GO:0015474 - autotransporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0046819 - protein secretion by the type V
                     secretion system [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /name="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(11096..>11301)
                     /locus_tag="BWI86_RS27435"
                     /old_locus_tag="BWI86_27465"
                     /pseudo=""
     CDS             complement(11096..>11301)
                     /locus_tag="BWI86_RS27435"
                     /old_locus_tag="BWI86_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            <11300..>11413
                     /locus_tag="BWI86_RS29755"
                     /pseudo=""
     CDS             <11300..>11413
                     /locus_tag="BWI86_RS29755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752107.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /name="alkaline phosphatase"
     gene            complement(11781..12068)
                     /locus_tag="BWI86_RS27445"
                     /old_locus_tag="BWI86_27475"
     CDS             complement(11781..12068)
                     /locus_tag="BWI86_RS27445"
                     /old_locus_tag="BWI86_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000222771.1"
                     /translation="MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAKLR
                     GLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYHLASERMK"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(12065..12257)
                     /locus_tag="BWI86_RS27450"
                     /old_locus_tag="BWI86_27480"
                     /pseudo=""
     CDS             complement(12065..12257)
                     /locus_tag="BWI86_RS27450"
                     /old_locus_tag="BWI86_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006688247.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /name="antitoxin"
     gene            <12894..16871
                     /locus_tag="BWI86_RS29270"
                     /pseudo=""
     CDS             <12894..16871
                     /locus_tag="BWI86_RS29270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569357.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /name="helicase-related protein"
     gene            16905..17346
                     /locus_tag="BWI86_RS27495"
                     /old_locus_tag="BWI86_27520"
                     /pseudo=""
     CDS             16905..17346
                     /locus_tag="BWI86_RS27495"
                     /old_locus_tag="BWI86_27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /name="peptide-binding protein"
     gene            17343..17591
                     /locus_tag="BWI86_RS27500"
                     /old_locus_tag="BWI86_27525"
     CDS             17343..17591
                     /locus_tag="BWI86_RS27500"
                     /old_locus_tag="BWI86_27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000747846.1"
                     /translation="MKKRYYTVKHGTLRALQEFADKHNVEVRREGGSKALRMYRPDGKW
                     RTVVDFKTNSVPQGVRDRAFEEWEQIIIDNALLLNAD"
                     /name="hypothetical protein"
     gene            17644..18684
                     /locus_tag="BWI86_RS27505"
                     /old_locus_tag="BWI86_27530"
     CDS             17644..18684
                     /locus_tag="BWI86_RS27505"
                     /old_locus_tag="BWI86_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="WP_024191723.1"
                     /translation="MLVKYIGESYMEKPWPGMHIRTSCLEDGFFRATQPKYLNDPSSES
                     RLLPFFNKFSPADYAWARNEFKKMQRDPSYVPSIQELEYYLKPCGKRYGEDFPHLLINE
                     GFTSMDSYDESKLQEIVTYLNDYLVEAVSCHLGVFSLSKSDCNLHMWTHYASIGKGFAV
                     VFDETHDFFKIYRPCDVSYNPEDRASVTYYKGAVRFNGYPAPSRNIDIKNKDNTLHGIL
                     NRDSVRELFINRLLYTKGEEWLPENESRIIFPLADCERKIGSIVSPSIDDCLLNEYPDV
                     FHDYHEINLKKIPFSAFKQITLGYNMTEKDKNIILDKVSSNKELSHVNVLHSKLDIYGK
                     VVVKPM"
                     /name="DUF2971 domain-containing protein"
     gene            complement(18782..19423)
                     /locus_tag="BWI86_RS27510"
                     /old_locus_tag="BWI86_27535"
     CDS             complement(18782..19423)
                     /locus_tag="BWI86_RS27510"
                     /old_locus_tag="BWI86_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016246529.1"
                     /translation="MDKKICVVSMSVGKPASMTAAWINNELIMAERTSYPERRRDMELQ
                     LLRELREKEEKGFIVLVEEENSFITGRVGQRVRLRDPFMNGRPVLIEAMQIYKELERQK
                     AIKLPRKESGKYILHQSIFDSEHDKKGDEFFNINWSEITTEHVLTLLCCFATEYNNVAS
                     ADYIRAMACEVDSRDDSSLIASYINIIKKTQIMGCARVPQGILTGKNNIL"
                     /name="hypothetical protein"
     gene            complement(19612..>19944)
                     /locus_tag="BWI86_RS27515"
                     /old_locus_tag="BWI86_27540"
                     /pseudo=""
     CDS             complement(19612..>19944)
                     /locus_tag="BWI86_RS27515"
                     /old_locus_tag="BWI86_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848366.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Ref family protein"
                     /name="Ref family protein"
     gene            20217..20991
                     /locus_tag="BWI86_RS27520"
                     /old_locus_tag="BWI86_27545"
                     /pseudo=""
     CDS             20217..20991
                     /locus_tag="BWI86_RS27520"
                     /old_locus_tag="BWI86_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691857.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /name="MBL fold metallo-hydrolase"
     gene            21007..21988
                     /locus_tag="BWI86_RS27525"
                     /old_locus_tag="BWI86_27550"
                     /pseudo=""
     CDS             21007..21988
                     /locus_tag="BWI86_RS27525"
                     /old_locus_tag="BWI86_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646431.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]; GO:0043565 - sequence-specific DNA
                     binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            22068..22397
                     /locus_tag="BWI86_RS27530"
                     /old_locus_tag="BWI86_27555"
     CDS             22068..22397
                     /locus_tag="BWI86_RS27530"
                     /old_locus_tag="BWI86_27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792637.1"
                     /GO_process="GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="WP_236923346.1"
                     /translation="MKNLCHPDFKFYSQVDTPLNSEQFIVQEKGHMDAFPGFTMRIHEI
                     FARDDKVACYLIFEGVQTREFLGHLASGRKVRFSLMFMITLKDGKYIEKRAHYNTADIL
                     RQLSA"
                     /name="ester cyclase"
     gene            complement(22504..23133)
                     /locus_tag="BWI86_RS28905"
                     /pseudo=""
     CDS             complement(22504..23133)
                     /locus_tag="BWI86_RS28905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722340.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            complement(23481..23774)
                     /locus_tag="BWI86_RS27550"
                     /old_locus_tag="BWI86_27575"
     CDS             complement(23481..23774)
                     /locus_tag="BWI86_RS27550"
                     /old_locus_tag="BWI86_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysogeny establishment protein"
                     /protein_id="WP_148116473.1"
                     /translation="MRKDFNIDGKYVVLSVSTNIQSPAVIVTVKLSDRMPDIDSISVAF
                     PVKSMRGAEHFVMNATEKAKVVSDFGELLDKVNNVLSISSARSKALTASMMK"
                     /name="lysogeny establishment protein"
ORIGIN
        1 gcattgtatt ttgattgttg aagtttaact ggaccgattc aaatcggaac ggagaaaact
       61 cagttgatga gaagaatgga tcactttcat caacgtgaat agtggcgata tggtgttcca
      121 gattctcctt cattaaatta ttttcgtcgt gcaattcatt aaaccagtcc tgaattgcct
      181 gcaccaattc ggggtgcctt accgcattaa ccgtcctgaa taatgatgaa atagcaatac
      241 ttacatcatc aaagcctcta attacggaac gattccgagc cgtaaggaaa cggttaaaat
      301 cgggagtacc tggcttaatg gttactgtgg ccatgccgta aagttcattt ttactttgca
      361 cgacttttcg ggtattgtca gatgcctgag aggtgcgggc atttacatca ggagttttct
      421 gctcggtttc atttattgat acgtcggtca ttgtgccacc tcatattttg agtatttacg
      481 gattttccgt cccagttcga tatatgcagc atgatagaat tcaagtaatt ctctgtaggg
      541 ttccagtaat ccctccgttt tttcgatcat ttcatattcc caagacgcgt gattaacaaa
      601 gcagcttttt gaatattttc cgtttttcat cgcagaaacc ccgtttcgac atatagctgg
      661 gtgccctggt tttaaacgga ctgaacttat gccaggtgat agtgactttt ttcgcattac
      721 caattaattc tagtttcggt gcatacgaac ccagatactc acgttttagt gtattgctag
      781 gccggggtaa attaatctcc agatgattcc gttctttcca ctcgatccag taatttgtac
      841 acaagtcttc agcctcctcc cgcagagcct cgatttgtga acgcattaag ctgcgcgata
      901 attcaagtga ctttattagt ttcgaattag acatgtgacc tcctgatgtg aaatcactat
      961 accgaagtgg tttttatttt tttttcacaa acaggacttt tttcagtgat ttttttctga
     1021 gaattatgag gggcgtgtcg ttggtgcgat gcaccgatag tgaataacac cagatgagct
     1081 cgggaacggc atagcggggt gtacttatgt tcggcagaaa agtagctttt tctgcaatgg
     1141 gtattcctga tccggtgatt tgaaaagtgc tgttttactt actaagttat acatatatct
     1201 agtgccactt tttaatcaaa agtgacccta ggttttttgc gcatagtgcc actttttaaa
     1261 aaagtgaccc tatgtcctgt ctacataggg tcacttttca caaagaagtg accctatgtc
     1321 gaggctccat agtgccactt ttcagcaaaa agtgacccta tgtaatagcc attgttaatc
     1381 aatcggaaaa atattcatgc tagctcgctc ttatcgctaa ggttattgct gctttctgcc
     1441 gataacagtg caagttttgc gattttttta agcgcttcac agttcgttag caagctcagt
     1501 ttttttgata aaattctggt cagtttgttt aaaaagtgtt acagtaaggc gaatggttga
     1561 atggttagtt ttaagactca aatcggcagt attaaaattc caaaagtagc ttttcatact
     1621 tcaaaaaacc aaactttaat ttcatgtaga agagttgtac tattgtattg actcaaggac
     1681 aatccgacat gtcagaatca gataacataa cgcgtaactt gctcggtaaa ctattacgta
     1741 ccgaggtttc gatttcgtag ttaccgtgca ttcctaatgt tattgcctat ccatgatctt
     1801 cttcgtatca atcagggtct ccactcttgc aacgccgtct atttggagac ggatttggga
     1861 caatgactga taatgcattt gaagttgaaa ctcgtccggg aagtttcctc gttccacgtt
     1921 ctttaggcaa agagatttcc tgggataatt ttttatggct gttgtcgatg gtgacactaa
     1981 cgttattcga gaatatgaca gtgacgatgc tgacttcggt atttacaatg ctgggaaaaa
     2041 gttactttac tgtggccggg aagactttta taatccgaca aaattcagca gttacgctct
     2101 aagtgtgtag atatacaaaa tgattacttt attcaaatct tctttatgtc aatgcttgca
     2161 cctgagtttg tcagcatatt ttttggatta aagcctgcaa ccgcagatgc aataaaagat
     2221 gttggcgttt catcacttaa agcaattaac gatgttgttc tctttccgcg aacaattgcc
     2281 tttacgccag gactggggat gatagcacta cgttaaaatc taaagtattc gcctgggcat
     2341 acgaattatc tgccgacatt cgtttgggta aagttagtga tgacttaatg gaactactgc
     2401 gttacgacac tatgtttacg tgtcaccgtc aggatgtgtt taactcatta gcaaataaag
     2461 tgctcttgaa ggattattga atatggctaa agtcgatcaa actaaagcta gcaggttatt
     2521 gagcgctaca aaaacgatgt cacttggtgc aaataaaaat tcaatcagaa tactttatgg
     2581 caatcatgtt gcagaaaata ttgatttcag gtccgaacct caacaacacg ttaaaaaagg
     2641 cattcgtggg tggagtcgtc gcgcggataa ccgcagagcg ttttctgctg ttattgatgt
     2701 cgctgtaaac tatttcggtt ttgatcctat tcatccggaa tccagctttc gcaatattga
     2761 ctttaacgca tcatgagcgt gctgcataac cttggttatt tcgtatgcgc cctgaggtaa
     2821 ataaataatt gaaattctta cgtcaatgaa agaaattgag gagctagaaa cttgtgcgaa
     2881 atgtaagtgc ccttacattg ttccatatac caacgatact cattgccctc ggtgtagatt
     2941 gtcgtcaacg gcatctgcaa tagtacggta ttcccaggag gagttagatt agttattact
     3001 ttatgtgagt ggttttaagc agagatcaaa aatctaaaga cggtttcgtc tttatctata
     3061 aatttttgac ttgcgtactt ttccacatgt actacatgtt gctgtttatt ttcagatagc
     3121 ttccgttctc acgcatatca agcatattgt gttaaatata actaccaagc gtagttgttc
     3181 tttgtttact ctatccttct tgccttcatt ttatgtaagc attactaaca taatttctca
     3241 ttctttaatt caatactgac tatgataaga cgatgttttg tggtgagact gcccaaccat
     3301 tacttctcgc tagtaatcaa ttataaaaat ccctacgtgg taataagcaa gttggggggg
     3361 cattcttaat ctgtgatttt gactgaatga tattgcggat tcacagttct gtactcttaa
     3421 atatttttat ccacttctct cttagagata aagttttacc ggggcacggt attaactgcc
     3481 gtttagtggg ggtttgtagt cacatttctt gttttttaac tcatgaaatc tacaccgtta
     3541 tcgaactctt ggtgtttttt tttatgccat cataccctat tccatgagga gatcggtgaa
     3601 tgactcgatt aacgttaata ccttcaaaga tgttagtgct gatgtctttg tttataactg
     3661 gggtcatgca aatgaagacc agtcccttat caaagacacc ccttcgtgtc aggacaggct
     3721 tttaagaaag gctttttctg gtatgacgac gcggcccgga aaactgaaga agaaattatg
     3781 gagaccacgc cctgagagat cccctcataa tttcccaaag cgtaaccatg tgtgaataaa
     3841 ttttgagcta gtagggttgc agccacgagt aagtcttcct tgttattgtg tagccagaat
     3901 gccgcaaaac ttccatgcct aagcgaactg ttgagagtac gtttcgattt ctgactgtgt
     3961 tagcctggaa gtgcttgtcc caaccttgtt tctgagcatg aacgcccgca agccaacatg
     4021 ttagttgaag catcagggcg attagcagca tgatatcaaa cgctctgagc tgctcgttcg
     4081 gctatggcgt aggcctagtc cgtaggcagg acttttcaag tctcggaagg tttcttcaat
     4141 ctgcattcgc ttcgaataga tattaacaag ttgtttgggt gttcgaattt caacaggtaa
     4201 gttagttgct agaacccatg gctcctttgc cgacgctgag tagattttag gtgacgggtg
     4261 gtgacaatga gtccgtgtcg agcgctgatt ttttcggcct ttagagcgag atttatacaa
     4321 tagaatttgg cattagattg gattgctttt agtcagcctc ttatagccta aagtctttga
     4381 gtgactagat gacatatcat gtaagttgct gataggtttc cagttttccg ctcctaggtc
     4441 tgcatattgt acttttcctc ttactcgact taaccagtac caacccagct tctcaacgga
     4501 tttataccat ggcactttaa agccagcatc actgacaatg agcggtgggt gttactcggt
     4561 agaatgctcg caaggtcggc tagaattggt catgagcttt ctttgaacat tgctctgaaa
     4621 gcgggaacgc tttctcataa agagtaacag aacgaccgtg tagtgcgact gaagctcgca
     4681 ataccataag tcgtttttgc tcacgaatat cagaccagtc aacaagtaca atgggcatcg
     4741 tattgcccga acagataaag ctagcatgcc aacggtatac agcgagtcgc tctttgtgga
     4801 ggtgacgatt acctaacaat cggtcgattc gtttgatgtt atgttttgtt ctcgctttgg
     4861 ttggcaggtt acggccaagt tcggtaagag tgagagtttt acagtcaagt aatgcgtggc
     4921 aagccaacgt taagctgttg agtcgtttta agtgtaattc ggggcagaat tggtaaagag
     4981 agtcgtgtaa aatatcgagt tcgcacatct tgttgtctga ttattgattt ttcgcgaaac
     5041 catttgatca tatgacaaga tgtgtatcca ccttaactta atgattttta ccaaaatcat
     5101 tagggattca tcagtgcgta acattacttt aacactttat gcttgtcatg atatttgctt
     5161 ggaagcctca tgaatacata ttctgataaa atacacaacc ttatagatat agccaaatta
     5221 gctgtggcca tgagggaaca tagctatttt ttcgcacttc gtcgaggaat agatgttaat
     5281 ttttgtgctg atttaaacgg gagtggtaca caagggatat tcataagaaa aaaaagcttt
     5341 aacgcgtatg agccaagttt tattgaagta atttttgaac ccactcataa aaacgatgat
     5401 tcatttctat acgaagagga ccttactacc gatcaacgaa aagactacga acctagtatt
     5461 aacagaggaa aacatcggtt cgttgcccaa cgtgctaaac taaatctcga ctgggacagt
     5521 aatgaaatcc aacaatggcg tttggatatt gagcgacttt ctaaaccaca caacactctt
     5581 aatgactggt tagaaaatga ttctgagata atgataatac atctctgcgg cggttatagg
     5641 ttccgtgaac cagtgatact ctctcaaaga gacattaaac aatatgtagc ttcaggattg
     5701 actctggaag acttaaaaaa tcggctaaag tgctcaatat gtggtgagcg aaacgccaaa
     5761 attaaagttt tttgatctac tttaatagga aatggcttgc ttccataacc taaagcaagc
     5821 cactacgcgt ttactttaag tacacaatta acatatcaga acagattatt tgtgcacaat
     5881 cagggagtta atatacgatc tggtctacat gatcaccaaa atcatcgtcg tcgtcgtcgt
     5941 catcatcgcc accatctact gctggccaat caacaaacca gccagcgtaa agatgcagcg
     6001 ttcggagaac atcacttgcg ggagcatcaa gggtgttaac gaatcccata tagctattgg
     6061 gatttgcccc agctatggct tcagcgatca tgtcctcggt aatgtcaccg gaaataatgc
     6121 ttaaacgccc ggaaacttct tcattatcat caaattcgat aatggcatct ccgcctaatg
     6181 gcgctgcgat tttaatctgc attatttggt gatgctgcca acttactgat ttagtgtatg
     6241 atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc
     6301 tactgacggg gtggtgcgta acggcaaaag cactgccgga catcagcgct atctctgctc
     6361 tcactgccgt aaaacatggc aactgcagtt cacttacacc gcttctcaac ccggtacgca
     6421 ccagaaaatc attgatatgg ccatgaatgg cgttggatgc cgggcaaccg cccgcattat
     6481 gggcgttggc ctcaacacga ttttccgcca tttaaaaaac tcaggccgca gtcggtaact
     6541 cgcgcataca gccgggcagt gacgtcatcg tctgcgcgga aatggacgaa cagtggggat
     6601 acgtcggggc taaatcgcgc cagcgctggc tgttttacgc gtatgacagg ctccggaaga
     6661 cgttgttgcg cacgtattcg gtgaacgcac tatggcgacg ctggggcgtc ttatgagcct
     6721 ttctgtcacc ctttgacgtg gtgatatgga tgacggatgg ctggccgctg tatgaatccc
     6781 gcctgaaggg aaagctgcac gtaatcagca agcgatatac gcagcgaatt gagcgcatac
     6841 ctgaatctga ggcagacctg gcacggctgg acggaagtcg ctgtcgttct caaaatcggt
     6901 ggagctgcat gacaaagtca tcgggcatta tctgaacata aaacactatc aataagttgg
     6961 agtcattacc gtaaccagag ttgaataaac atccattttt ttgtgttttt ttaacctccc
     7021 gggtttttaa ttcctgtcga ttaaaagtgt tagattattt ggttaagagt tctctcgtcc
     7081 tatttattca ggcgttatca cggatatgca gatcgctaag tcagttgtaa gaaaaccaga
     7141 cagtgcacat cattatacct aggtcttctc tggaaaaata cttagaataa tttatgtaaa
     7201 acaggttgtc gtcttcatga ttttaaaacg cagtacatat atatactcta taataaatac
     7261 agggagtatc acgagacgtc tggataagag gaataacaga ataaatggta aggaataaag
     7321 acacttattt ctattgagaa aaaaacattc atcctgatgt gattagtatc aatgaaataa
     7381 tgtgcttgta tggttgatga aaaatgcatc actaaagaaa aacagtatga ataagaatat
     7441 acgaatttta caatttctgg tcagtataat ttattctgtg cagtctcatt tttctggtgc
     7501 gcaaacaata caactgaatg gtaatggtat acctgaaagt ataaccagga gtattacagg
     7561 tgttgatgga aacgcggcgc ttaatatcag cgtgccgtat aaaacaagtt atactcaaaa
     7621 tatactatct gttgaaagca gtattaacat tcaaaggagg aacaagcaat acatcaatcg
     7681 gtggtgcagg tgtctacggt gaaaacttta cgctaaataa taatggaagt gtttggggag
     7741 gagatggata taatggtgga attgctgtta gtggcaacaa aatatctata aacaattaca
     7801 gaaatgtata tgggggtaat ggtcttggtg gctcaggaag tagcggaggt gcagggttaa
     7861 gcggggatga tattatagtt gataattaca gaagtatata cggaggtgat gatgtaggtg
     7921 ggacagcgtg gttccggtgt aaccggtagc aatattacag tgcataattc cggaggaata
     7981 ttgggcggta atggcgtaac ggtggtgatg gtattaatgg tagtaatctt ttcattacta
     8041 acgacaacat gatatctgga ggatatggaa taaaacaagg ggagatgcta tttctggaaa
     8101 tcaaatcact ttgaataata acggtattgt tcagggggat atggccccga cggtggttgc
     8161 tctgtttatg gagaagatat ccatattaat aatcatggta atctttcagg attatataat
     8221 agccaaaaag atgcttataa tacatcaata attttttctg gcgggtataa ttcattagat
     8281 attcattcag gctctgtgat taatggtgat attaaactag ctagtatacc tgttaatggt
     8341 acaaatgaat taattattaa aaacatcaat aaacgcaaca gcaattaatg gtgggctaat
     8401 gattgggaat ggctcatctg tttatctgtc aggcaagaac tccattttta acggaaatat
     8461 aagtattgat gaagacgcat ctatgaacct gtctgtagga aatgctaatg ttcacgcaat
     8521 actattacat taaaaagtga ttcatggctt aatatagaca catcaattaa gaactggact
     8581 caggaactat tacacattat tgtcgtcaga cacaggtatc tcgatcgctg attaatagtc
     8641 acattgtaca atacaatgca ttactgacgg aaggtgctga aagttatgtt tatacgtctt
     8701 taaatgacga cgataacaaa ctgatatcca tgctgagatg gaataataca aaagcatggg
     8761 atatggaacc tttaatatag aaaaagatgc cactctgaac ataggcgttt ctctttccga
     8821 taatctttca cctttattat atgatggctg ggacggcaaa agtctgacaa aatcaggtaa
     8881 tggtactctt atactttctg caacaaacaa ttatacagga aatacagagg ttaaatctgg
     8941 cgtattaatt cttgctgcac ctgatgctct tggtcggact gagtatttat atttatcccg
     9001 tggcgcagaa ctggatatga atgggtatcc tcagacaata aagcaaacta ctgacggctg
     9061 caggctctgt gctgaacatt catggcggaa gcctgataac tgaataatgg aggagaatct
     9121 gcaggtacta ttgcagggga tggttctctg aacataaatg gggaatgctt gatataacgg
     9181 gtaataatcg taatttttcc ggtgttttta ccgtgaataa gggggctcat ttggctgtat
     9241 ccacggctga taatctggga cagcctttgt tgataactat ggcacattaa ctctgaacag
     9301 taacatcagc atgcagctta ccaacaatat cagtggttat ggtaatgttc gcaagacgga
     9361 gcaggtgcac tgaacattag cgataacgca aaaatggacc gggatgacag atattattca
     9421 gggacagtga tactggggaa cgcagattca ccggtgatgc tcggcagtaa ccaggtcatt
     9481 gttgaagagc agggcaaact ctccgggttt gggggcgttg caggaaatct gagcaatagt
     9541 ggtatagtcg atctcactac atatatgccg ggtaatatac tgactgtcgg agggaattac
     9601 actggcagaa atggacttat tctcctccag acagaaacag gtggtgacaa ttcgaaaaac
     9661 agatcgtctg gtgattaaag gtaatgccag tggccgtacc cgtgtcgctg ttactcaggc
     9721 cggtggtact ggtgcagaga cacttaatgg attgaagtga ttcacgtcag tgcaatgctg
     9781 ataatgctga attcattcag acggaacgta ttacagccgg agcttatgat tacatactga
     9841 aacgtggtca ggggattaac agcactaact ggtatctgat tagcagaaaa gacattcctg
     9901 taccacaacc tgaagctgta ccggaaaacc atgataataa tttgcgtcct gaggcaggta
     9961 gctatgttgc cagtattgct gctgcaaata atctgtttgt aacgaatctg tatgaacgac
    10021 agggacagga gttgtatatc agccacatga caggagaaga aaatgaagca ggtatctgga
    10081 tgtataataa aggaaaacat aatcgctggc gtgacaacag tagtcagctg agaacccggg
    10141 gaatagctac gttgtgttaa tagggggaga tatagctcag tggagcctga atggtaccga
    10201 tcgctggcat acaggtatga tggctggcta tggtcataat aataacagta cgaatgccct
    10261 gagcaccgga taccattcgg aaggaagaat gaatggatac acagcgggtc tttatgcaac
    10321 atggtatgcc aatgatgaaa cacacaatgg ttcttatctt gatagctggc tgcaatacag
    10381 ctggtttgat aatcatataa atggagaacg gctgcctgct gagtcatgga agtcaaaagg
    10441 gtttacggta tctctggaag caggatattc atggaaggct ggagagttta ccgacaatta
    10501 caagggaagt catgaatggt atgttcagcc gcagcttcag gttgtccgga tgaatgtaaa
    10561 atcagacaaa tatcatgaaa gtaacggaac cagtattgaa aataccggta acggaaatat
    10621 tctcacccgc ctgggagcaa gaacatggct taccagcaaa aacggtaaaa atacgcggta
    10681 tgcggttccg ttcagaccat ttgtggaggc acactggttg cacaatagtc gtgttttcgg
    10741 caccagtatg aatggtgtaa gtatatacca ggatggtgcg cgtgatatcg gagaaataaa
    10801 tggtggtgtt gtgggaatga taacaccaga agtagcattc cgggctgatg caggcattca
    10861 acttggagaa catggatacc ataatacatc tgccatgttg agtgtggaat atcgtttctg
    10921 atgtgtcttt acattataaa tatccttcac aaaatattgt tgataagaca tagttaggtg
    10981 gtgagctgcc tcggaaaata tgacgacagt aatgatttct aagagatttg aaatgcgttg
    11041 ataacaaaat ctgaaatata ggagtctgtt taggattgtg tgtaaatgcc ttttctcaaa
    11101 agtaaccgtc caggcggtca ctgaatccga taataaagcg gcttattgcc ataccccagt
    11161 ccttcaacgg catcgtccat ttcggggacg atgcctgtat tgccagacac accacttttt
    11221 tcaccggctc gtctgtcgga aacacttacg ctttttgatc gcatggcgga tcacgctgtt
    11281 tagcgacccg atggcattcg tcagcgacag cgacaactct taccgctgga acgaaatgga
    11341 taagcgcttc agtgtcgacg acacgccgaa cgagccgaac cgcttcggct gggtggggga
    11401 aatcgatcca tatggttatt tttcagaaaa taataagaac atctgtgctt tatataacca
    11461 gataaaacca cgcaataagc ataaatatta aaggtgataa tacttttatt tatcacttcg
    11521 gctatattca ataaacataa taactttctc tgaaatcaca ggtccggcca ttgcagttgt
    11581 ttcagacgtt gattaggatg ccaatgcatt tgcagggatg gtttgtgtat ccaccgggta
    11641 taatgcgtga catcattaat ttcaacactg actgtgcgat tgtctgttat gcctgcccac
    11701 agggtaggct ttttttctgc atgcaggaaa agaatcactg aaaaaatgga agcaggtaag
    11761 aaagagtgct gaccaggata ttatttcatc cgttcactgg cgagatgata aacgttgctg
    11821 cgttcacgtt tacctactgc gacgaccgca ataattaaca tatcatcaat aacctgataa
    11881 accagacgaa aacctgatgc gcgaagtttg attttatagc agtcctttaa tccacgtagt
    11941 tttgcagaag ctatatgagg attttcactg cattttttca gtttttttgc gaactgctgc
    12001 tgaatggtct tgtccagtct gagccattct ttcagtgcat catcccggaa ttttactgta
    12061 taagtcataa ataactgtcc aggtccacat catgcagagg ctgactgctg cgttcagtaa
    12121 ccagttttac cagctcctgg tcatcccaag gcatcaagca ttctttcata gagctcagca
    12181 gggacacagt agaatgctgg ctgattccgt taagaatggc aacaggatac cccctctccg
    12241 gcactgacag ttgccatcgg attttttttc agctcactga cgcttgcact ggtgtcagac
    12301 aaaatgatac tggcataaca cacctctgag actatttaac agtcttcaga ttacaccctg
    12361 aaagactgct taacaagtct gttgatgtta atgtgcaccg tcactcttca attgtcctga
    12421 tgccgcgcca gcggcatgga aggcggcact ctgttgtttc atatgatacc ggagtaaaac
    12481 cgccgaagcc cggcgtaagc cggtactgac tgatagattt caccttaccc accccggccc
    12541 tgctaagcca tacccgtttt caagccatga cagagccttt atgcgcggtc ggagtggtcc
    12601 cgacgagggt ttacccgaag tcgggcgtat ctccgcgtta gcgggccgtg agggccgctt
    12661 acgagcgtgt tactgaggac ttccagccag aagactgaca gcgatgacaa tgtacttaca
    12721 acattcataa ttaaaagcga tgttgttccc gcccttcggg cgggacggcg gcgcttttca
    12781 gttatgaggg ggggtacgtg ctgtgtttgt gtggtgtttt ttattcctga tgtttttttt
    12841 ttgttgtggt ttcagaacgg tgagattcgg ccgcgcgtta gcgtggaggt gctcctgttc
    12901 ttggtactga tggcactcca gttatgcgcg atgccctaaa ctggcagatg aagccactat
    12961 cacggcgcgg atgaacgcga tcccgcactc aaattaccgt gcagtcgtgt ttaccaaaga
    13021 acaatacgcc cgcactccgc tacgtgatga caccgtagat gagcatgcac aggatatgct
    13081 ttatgacttc gttgccgccg ggcgcgtagc cagcgcatgg actccgactc cccaccgcaa
    13141 agaggccgcg cgtcgccggg tattgtcgga gtattcagat accggtaccg aaaaaagcag
    13201 agaagtatcc gtactttgag gatatgggct tcgatagtgt gatcgctgac gaaggtcaca
    13261 actaccgcaa tagctataaa aatggtcgcg aagcgtcaca actggcctat ctgccaccag
    13321 cgcggtggcg caatcggcgc gggatatggc aattaaaaac gcgtacctga tgaaaaagaa
    13381 tggtgggcgc ggaccggttc tcctgactgc aacgccagtc gttaacaccc caatcgatgc
    13441 atacaacatg ctttctcatg tgctgccgaa ggaatactgc agaagatggg gatctacggt
    13501 cctgatgact tcgttaaatt cttcgggcaa gaccaggctg gaaacgtaca gaaaatcagc
    13561 ggtgaagttg aagaaaaaat ggcgctggtg ggctttgaaa accttgatgc gctgcgcggc
    13621 aatattccat cgctggacaa cgcttaaaac ggcggaagac gttaaggata ccgtggagat
    13681 cccggaactg gacgaacacc agcaggatgc accacttact gaagaaacaa ctggcggcgt
    13741 atgaagaatt acgccagcag gcggaagcgg tggccaaagc caacaatggc gtaacgacct
    13801 cggtcgatga agacggagtg attgagcacg agaaagcccg ccgatcttct caataatcag
    13861 ggatatggac cgcgtatgta ctgacatgga cctgtactat cgccggatca cctatcgttt
    13921 cctgccggag tacgccgatg cggtgcagca gctggcggac agtttgccta aacaagcacc
    13981 agcgaagacg acgacagtga tgattcaatc acgcagcaat cgcaatactc cctgatagat
    14041 aaggcgagtt tattcagttg caggttccgg aagcgttcga gcaggaaagt gaataagcgc
    14101 ctggccaggt ttggcattga cgaacagacc gtaactcacc ccgttacgcc caaatacgcg
    14161 aagctgattg ccacgctgaa ggagtttttc ccggaaggga aagcaaatca tcttcaccga
    14221 cgaaaaaacg cagcaccaga agctcaagcg cattatctgc caatgctctt aaccttgaac
    14281 cttcaaaggt aaggatcctg aatgctcaga cggtttgccg aggcaggtaa aaccggtaag
    14341 aaactgaaag cggttaaacc gccgaaagaa gttaccggat gaaccaacag atgcacagat
    14401 agcgaaatac aacgagcaaa tggctctgta tgacgcctat atcgcgcagc aaaaatgaaa
    14461 tgtcgttggg cggtctggaa aagattgcag ccgacttcca ggagggccgg actccgatca
    14521 tcatctgcaa caaaaaggca gaggtgggta tcaacctgca tcgaggaacg actgacatcc
    14581 atcatctgac gttgccatgg acaccagcca gtgtcgcaca gcgtaacggt ccgcggtgcc
    14641 cgagttggct ccaaccgtgc aagcgtttcg cgttcattac tactgcggca agggttcttt
    14701 cgatgaatac cgactgaaga cgctgaagcg taaagcaggc tggatctccg atatcctccg
    14761 ttcagataag tcagaaatga gaacgccgac gccaacgata tgatcgaaat gcagatgtat
    14821 accgctaagg atgatggcga acgtctggca atgatgcagg ttcaaatgga taagcgaaag
    14881 ccgcgcaacg cgccgccaga aagaacaggc cgctatcgac cttcagaact aacatcaagg
    14941 cgcaacatgc agctggcgag gatgttgcgg ctcttaccgc tgaattagag cgaagtaaaa
    15001 gcggaacttg aaaagaccac cgccgaggta gctaaattca aaacaggcgg taatggccaa
    15061 agcagctgat aatgcagact ggaagcccgc tggggtagcg tccatcacac agaccgtacg
    15121 ttgttagcac attatcgcgg cgtcgttgaa aagcgccatt cagcgcaagg ctaatatcac
    15181 tcaagccatc tcccgctatg agaaattatt gaaccgtact cagaaggccg cgacggatat
    15241 caaacgcctg cgcccgctgg tggaggatgc aataaataaa ggcattctgg atgttgatcc
    15301 tgatctggtt aaccatgcga atgagttcct tgttatcggc gatcgctcat ggcgtgtagg
    15361 ccaatactac gatttgtgcc ggtgatatcg ttcgcattaa gtcgctggac ttcgacagcc
    15421 agcgcgcaga cgtggagatc atctttacct tcaaaggcac caaatcgggt aactgggatg
    15481 tgaagacgct ggataaacag gtggatgtaa ctcccgatga agatgctgtt atgcagaaaa
    15541 tcagtggtgg cgtctccatc gccgggatta acgacatcat ttcctgtgac gatttctacc
    15601 gtttccagca gcgcggcatg atcaaaatca ctgactcata cggcgttcag actacagagt
    15661 caggctatag cattgatttt gttggtacct atacggaccc actgaagcat gcggtttacc
    15721 cggatcgccg tgacggcgcg ctgaagtcgt caattgcaaa atgggtgctt ggtatgatgt
    15781 cggaagggaa taaccgccag atccgttcgg cagaaacatt cctggttgaa ctgtttggct
    15841 ccaattatgg cgatgtaatc gcgtcatacg gagattacgc tatcccctga agcaattcag
    15901 gagaaaatag cggatgcgat cgccagaatg ccggaaaaaa caagccaggg ggctactcgt
    15961 aacggggatt ctgaacttga agtcaccaat gccattttcg gtaccaatga gttccgggcg
    16021 tcagattatg agatcaccac agcacagttt ggcaccattg gcatttacag caataaagac
    16081 gagatcaagc aggcaatgga cgcagcaagt gcgcgcattg cagcagaacg ggaagccaat
    16141 ctgaatcatg cagtcgccgc gctgactcaa tcgtgggtaa cagcaatcag ggaggccgcc
    16201 accacaggga aaatcacacc ggcaattgcg gatgtcgtaa acgacggctc taaatttatg
    16261 gatgcctatc aaatggatgc ggtgaagttg ccatcagcct atggtcaact cactatcgca
    16321 tgacctacaa cctggtatca atgttttctg accttgcatt cttgggctgg tggaccttaa
    16381 acgaggttta cgccggaatt gctcagcatg cgcaagaatc atgtggagat attgcagaga
    16441 attaacacgg cacttgccgg tcgcaccgat gaagagaaac aggccgacgc tgatcggata
    16501 aaacctggcc cttggcaaca tcacggagga agaaattgcc gccagaaacg agaaacaaga
    16561 agagttatca tcaatacagg tgatgccacc agcatagctc agtctcttgg tctgaattat
    16621 cgcgtatcca ccgccgacct gaagatgatg tacgcaccaa aattcgccgc tggcgaggta
    16681 tttgggcttc aggaagcctc cggcatgaaa aggcattctt ttccgtgcga aagacgcaat
    16741 caaggcgaaa ttcggcgctc gctggctgcc agcgaaggcg aagaacagcg atttcccggg
    16801 caactggtgg attatcgaga caaaacacaa cgtggcggac gttctggccg tcaatccaac
    16861 aatacgcata acaggagcgc ccggttcgcc gggcgtcgca taatatggcc acactatctg
    16921 atacaataaa accgaataaa acatatcttg aggcggtact ccgtacagcg ttgtttagga
    16981 aagacagaag acgaatacgt tgatttcttc ctgtcagggc tacgcgggcg attactgaaa
    17041 aatccccgcc tgtaccgcag ctatggtcca tactggccgg aaattaaaaa attattactg
    17101 gagcgcggtt atggtaattt cggtcgtctc gttgaccgtg acgttcgcaa aatttaccgt
    17161 tatgaccgcc cggcgctaac actcatagcc gcgacgctct acagccagga gcgttttgat
    17221 aatggtcaga tatactcagc ctggcattta ctgccagtgc ctgaagaagt tgacgaccag
    17281 gactatgagt ttgagtctta cgatttggaa gttgaagcct tggcacaggc tggagagaaa
    17341 acttgaaaaa gcgatactac acagtaaagc atgggacgct acgagcattg caagagtttg
    17401 ctgataagca taacgttgag gtgcgcaggg aagggggaag taaagctctg cgcatgtacc
    17461 gtcctgacgg aaaatggcgg acggtcgtcg atttcaaaac taacagtgtt ccccagggcg
    17521 ttcgcgaccg ggcattcgaa gaatgggagc agatcatcat agataacgca ttgcttctga
    17581 acgctgatta ataatcattt aaagcccata tgatatgggc ttaaacaaca ggatcgatat
    17641 acagtgttag ttaaatatat aggcgagagt tatatggaaa aaccttggcc gggcatgcac
    17701 attagaacat cttgtctgga ggatggtttc tttagagcaa cgcaaccaaa gtatcttaat
    17761 gatccgtcga gtgagtcgcg acttcttcca ttttttaata aattttcacc agccgattac
    17821 gcatgggcta gaaatgaatt taagaaaatg caacgagatc catcttatgt cccatctata
    17881 caagagttag agtattattt aaaaccttgc ggtaaacgat acggagagga ttttccacac
    17941 ttgttaatca atgaaggttt tacctcaatg gattcatatg atgaatctaa actccaagaa
    18001 atagtaacgt accttaacga ttatttagta gaagccgtaa gctgtcatct tggtgtattc
    18061 tctctatcaa aaagtgattg caatctacac atgtggactc attatgcatc gataggaaag
    18121 gggtttgctg tagttttcga tgaaacacac gattttttca aaatatatcg gccgtgtgat
    18181 gttagttata atccagaaga tagagcatct gtaacctatt ataaaggcgc tgtgcgattt
    18241 aatggctatc ctgcgccatc gaggaatata gatataaaga ataaagataa tacattgcat
    18301 gggatcttaa atcgagattc agttcgcgag ctttttatta ataggctcct ttatacaaaa
    18361 ggtgaagagt ggcttcctga aaatgaaagt agaattattt ttcctttagc tgattgcgag
    18421 agaaagatag gaagtattgt ctctccatcg attgatgatt gcctattaaa tgagtatcct
    18481 gatgtttttc atgactatca tgaaattaac ttaaagaaaa ttcctttttc tgcattcaag
    18541 caaattacct tagggtataa catgactgaa aaagataaaa atataatttt agataaagtt
    18601 agttcgaata aagaattgtc tcacgtcaac gtattacatt caaaacttga tatatacgga
    18661 aaggtcgtgg ttaagcccat gtaataatac gcttatgtct acacttcatg ataaatacct
    18721 tccttaactt aggaaggtta gttgtcggtt tacgcagaaa gtaacaaata accttcgtag
    18781 gttatagaat attattcttt ccggtgagta ttccttgagg aactctagcg catcccataa
    18841 tttgagtttt cttgatgatg tttatatatg acgctatcag cgatgagtca tcacgactat
    18901 ccacttcaca agccattgcc ctgatgtagt cggcgctggc aacgttgttg tattccgtcg
    18961 caaagcaaca tagtaacgtc agaacatgct ctgtcgttat ttcgctccag ttgatgttga
    19021 aaaattcatc gcctttttta tcgtgttcgg aatcgaagat gctttggtgg aggatgtatt
    19081 tgccggattc cttgcgcggt aacttgattg ctttctggcg ttccagctcc ttataaatct
    19141 gcattgcctc aattagtacc ggcctgccgt tcatgaaggg atcgcgcaac cttacacgct
    19201 ggccaactct accggtaata aagctgtttt cctcttccac cagcacgata aaaccttttt
    19261 cctctttctc acgcaattcg cgcagcaact ggagttccat atcgcggcgg cgttcagggt
    19321 agctggtccg ctcagccatt atcagctcgt tgttgatcca tgcagcagtc attgacgccg
    19381 gtttgccgac gctcatcgaa acaacgcata ttttcttatc catagcgccc ctacaaaaaa
    19441 gaaaagccac cagcggcggc ttagcaatac aactgaaggt agcgcccggt actcagactg
    19501 tgccgtccat ggaatatttg aaaagggatc catccgtacc gggcatgtga tgattctgac
    19561 tgaagtcact tgtcagttgt caattatttc agattaaaaa taatatattt attagtgcat
    19621 gatgtttgcc atctcatagg cgtcagccag caactccatc tctgacttgt tcagcaaggt
    19681 gaattctttc ttgcctccaa ccacaccatc ggcatgaaca gggaccagcc aggggtattt
    19741 ttctcttact tcagccggtg ctgcatgctg gtggtgccat ctacaaaggg gcaattgctt
    19801 tttgtgacaa cccggcgcgg tacgaccgga gatatggtgc agagatacct cttctgatat
    19861 tactccatgc atgtagcagg caatgcaggg gagagcgcca agagcattgg cgatgcgccg
    19921 ttcctccgtc gttggtgggc tccgtttggg aaacggaaga tattgtacgt taagatgatt
    19981 ttttgtactg gctgacatca ataagcctta aaggacgata cttcccttca atgcgtcaga
    20041 tctgtacccg ctttcagcag acatattcct gaaacctgag agccagaaga atggcctgat
    20101 atcatccaaa tatggcatta tttcagtggc aggcaggcct gatttaatca taattatcac
    20161 attaggccat catgatatgg ctctgatggt tattaaccaa tacaggactt gctaaaatga
    20221 agatcatcca ggtacgtaac gccacacaac tcatttctta cgctggtaaa aaatttcttg
    20281 tcgatccgat gcttgcgaaa aaagaagcct atccgggatt cgaaggaacg gcccggtcag
    20341 atatccgcat cccgatgact gagctgcctt ttgaccttga tgtactgctg gatgtggatg
    20401 ccattattgt cacccacaca caccctgacc actgggatga agcggctgtt gcgcacatcc
    20461 cgaaagaaac tgatttatgt tcagaatgaa aatgatgaaa agatcctgcg ttcccagggc
    20521 ttcagcaacc ttgttattct gtctgacaaa agcacatttg gcgaaatttc acttattaaa
    20581 accgtttgtc agcatggttc ggacgaagcc tatgctaacc cgcaactcgc agcaatcctt
    20641 ggggatgcct gtggggtggt cttccagcat ccgtccgaaa aaacgctcta tctggtcggg
    20701 gataccatct ggatcaaggc tgtagaggat aatttacgtc ggttcaatcc tggcgtcgtt
    20761 attatgaata cgggctgggc gcatgttctc ggattcggcc ccattatttt cggcagggaa
    20821 gatgtcctga aagcccatcg tatcgtacca gaggcactga ttgtggcaac gcacatggag
    20881 gccgtaaacc actgtctgct gacccgtcag gaactgctgg aatatgcccg cgataaccag
    20941 atccaggcgt cagttgccgc tcctgctgat ggtgaatcag ttacgattta acccggaggc
    21001 catggcatgt ccaccatcac tgtcgctata gtgatcaccg aagggttcag tacatttcat
    21061 gtctccgttc cactaatcct gttcggggag atactggaag ggaaggctct cttcagccgc
    21121 cgggtatgtg cggaagagcc cgggcttatc tggtctgctg aaggtacggc gatgagggca
    21181 gaatacgggc ttgatgcact ggcagaggcc gatatcgtta ttcctttctg gaaacacatc
    21241 catgaaaggc cttctgaaac tctgctcagt gccctggttg cagcctggaa aaatggagct
    21301 caggtggtgg gcctgtgggg agttttgttc tggcttacac cggtctgctg gatggtaaac
    21361 gggcggccac gcactgggag gctgaacggg atttccagat gcttttcccg gcggttcatc
    21421 tggacgtgaa cgtgctttat gttgaggacg agcggattat cacctcagcc ggtacagccg
    21481 cagcactgga ctgttgtctc catgtgatcc ggcaacgcct gggcagatat acggctaatc
    21541 aaatagcccg ccggatggtt gctcctcctt accgggaagg cggacaggca cagtaccggc
    21601 aactgcttat tcccaaaagt acgtccgata accgcataaa ccagctgctg gaatacctgt
    21661 ccgcaaatct gggattacca catgatattg acactctggc ccatttagcc tccatgagcc
    21721 gaagaacgct tacacccatt ttcattcggc cacaggaatg acggtctggg agtggatcac
    21781 cacagagcgt ctccggcgga gtcaggaact gctggaaagt acagatattg cagtggagcg
    21841 ggttgccgaa ctggcaggct ttcagtccac cattactttc cggcagatat tccggcagcg
    21901 gatgggagtc agtcccagtg aatggcgaaa aacatttaac ggaacgccgg aaggcaatat
    21961 tccggcaggt aaagaaactg aaaaatgagg tgcaatatgt ctgttgaaga aatatcaaaa
    22021 cggttaaaaa gttttatcgt ctgattgaag aaaataaata tgatgaggtg aaaaacctct
    22081 gccatccgga ttttaaattc tattctcagg tggatacacc gctgaacagt gagcagttta
    22141 tcgtacagga aaaaggccat atggatgctt ttccgggatt tacaatgcgt atccacgaaa
    22201 tctttgccag agacgataaa gtcgcctgct atcttatctt tgaaggcgtg cagacccgtg
    22261 aattccttgg gcatcttgcc agcggcagga aagtgcgctt ttctctgatg tttatgatca
    22321 ctctgaaaga cgggaaatat atagaaaaac gggctcatta taatacagcg gatatcctcc
    22381 gacagttgtc agcctgagtg gcaacacatt gcccagtctg tattattaca ggcagggctg
    22441 cgtatctgac aggagcaatg agcttagcct ggaaaggtaa tccattaacg cctgggctac
    22501 tttttattcc gattcaggct ctctataaag ggcttgaatg cggcatatct gtgaaggact
    22561 gtaccctgtc gcatctgccg tttcatggat acttagtttc ttgacctgcc ggtagtacag
    22621 gactttctga tgccgttcct gatcggcctg ttatccccgg tatttaccca gcgtatgagc
    22681 ccgttcaatt ccctgttgtt gccgttggcg acggctcagc cagtccttat gcgacatcgc
    22741 ggccatcagg tcgataagca tgttattgat ggcggttatc acggcgcggg tgattgggtc
    22801 agcctgcgaa gggtctttat ctgacagcgc ctgccatgag gtggatacat ccaggcgaca
    22861 atccgcagtt cgtgttgttc tatctgcttt ttcagcgtaa tccagtcact gttactcaga
    22921 cgggtcaggc ggtcaatttg ctcgaccagt aaaatatcat tgcggtggct gtccatcagt
    22981 aagcgtccca gttccgggcg gtcgagtttt gttctcccgg tagtaactgg ctattttatg
    23041 tccccgatcc tgaacaaact gccccagcat ctcttttgcc cgatcggcaa actgatcttc
    23101 cgttgatgcc cttaaataag ttctgatgaa catttttcca ccacgttatc gcttttaggt
    23161 tattgcattt aatctatcgc aatggcagag tcctgtccgg gaacctcgcg ggcttctctg
    23221 gaaaacatgc aggcatggta cttccgagat gaaacctact ctgctgccct ggcggaactg
    23281 gttaagtagg taatggccac gtcttcgtag ttgactaacg ccacggctca attataacaa
    23341 tcaattagag caatgttatt tgtttatctt cagtcacatt ttttcacttt agtatctatg
    23401 tgctatactc ctttctgatt gattggatgc ggaacacaaa cccgctcttt tgtgcagcct
    23461 ggttccttgc caggcttttt ttatttcatc atggaagctg ttaacgcttt ggaccttgct
    23521 gaactgattg aaaggacatt gttaacctta tccaagagtt cgccaaagtc agacactact
    23581 ttagctttct cggtggcatt catcacgaaa tgttcagcac cacgcatact tttgacaggg
    23641 aacgcaacgg atattgagtc aatatcaggc attctatcgc tcagctttac agtgacaatg
    23701 acggctggcg actgaatatt agtgcttaca gacagcacta catattttcc gtcgatgttg
    23761 aaatcctttc tcatatgtca ccataaatat caaaaaatta gaacaatcat ttgcgcgtta
    23821 atggctaatc gccatcttcc agcaggcgca ccattgcccc tgtttcacta tccaggttac
    23881 ggatatagtt catgacaata tttacgttgg tccagccacc agcttgcatg atctccggta
    23941 ttgaaactcc ggcgcgggcc atatctcgcg cggcaccgac acgggcacta tgtccagacc
    24001 aggccaggta tcgctgacca gagtcatctt ttgccccgta aatcaatcgg tgagttgctt
    24061 caaaaatccc ttccagggcg cgagttgata gctggctggt ggatgatggc gcggtaacac
    24121 cattttttct gacgcggcaa aacaggtagt tattcggatc atcagctaca ccagagacag
    24181 aaatccatcg ctcaaccagt ttagttaccc ccaagctaag tgccttctct acaccagcgg
    24241 agctaaccag cgttttcgtt ctgccaatat ggattaacat tctcccaccg tcagtacgtg
    24301 agattcttta accctgatcc tggaaatttc ggctatacgt aacagggtgt tataagcaat
    24361 ccccagaaat gccagattac gtatatcctg gcagcgatcg ctatttttca tgagtgaacg
    24421 aacctggtcg aaatcagtgc gttcgaacgc cagcgcctgt tttgcacgct caccggcatc
    24481 aacgttttct tttcggatcc gtcgcatgac cagtgaaaca gcattgctgt cacttggtcg
    24541 tggcagcccg gaccgacgat gaagcatgtt tagctggccc aaatgttgct ggatagtttt
    24601 tactgccaga ccgcgcgcct gaagatatag aagataatcg cgaacatctt caggttctgc
    24661 ggaaaccatt tccggttatt caacttgcac catgccgccc atgaccggca aacggacaga
    24721 agcattt
//
