LOCUS       NZ_CP019148             5518 bp    DNA     circular CON 07-APR-2024
DEFINITION  Acinetobacter lwoffii strain ZS207 plasmid pZS-7, complete sequence.
ACCESSION   NZ_CP019148
VERSION     NZ_CP019148.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06191591
            Assembly: GCF_002119785.2
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Izak,D., Jurkowski,M., Gawor,J. and Zielenkiewicz,U.
  TITLE     Draft genome sequence of Acinetobacter lwoffii ZS207 strain,
            isolated from arsenic compounds waste
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Izak,D., Jurkowski,M., Gawor,J. and Zielenkiewicz,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2017) Microbial Biochemistry, Institute of
            Biochemistry and Biophysics PAS, Pawinskiego, Warsaw 02-106, Poland
  REMARK    Annotation added by database at submitter's request
REFERENCE   3  (bases 1 to 5518)
  AUTHORS   Izak,D., Jurkowski,M., Gawor,J. and Zielenkiewicz,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2017) Microbial Biochemistry, Institute of
            Biochemistry and Biophysics PAS, Pawinskiego, Warsaw 02-106, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 135.0x
            Sequencing Technology             :: Illumina MiSeq; 454; Sanger
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002119785.2-RS_2024_04_07
            Annotation Date                   :: 04/07/2024 16:03:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,515
            CDSs (total)                      :: 3,403
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 55 of 137
            Pseudo Genes (incomplete)         :: 83 of 137
            Pseudo Genes (internal stop)      :: 39 of 137
            Pseudo Genes (multiple problems)  :: 35 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019148.1.
            Bacteria and source DNA available from Urszula Zielenkiewicz,
            Department of Microbial Biochemistry, Institute of Biochemistry and
            Biophysics PAS, Warsaw, Poland, 02-106, Pawinskiego 5a.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="ZS207"
                     /isolation_source="microbial mats from Zloty Stok gold
                     mine"
                     /db_xref="taxon:28090"
                     /plasmid="pZS-7"
                     /geo_loc_name="Poland: lower Silesia"
                     /collection_date="2012"
     gene            complement(109..753)
                     /locus_tag="BVG18_RS17095"
                     /old_locus_tag="BVG18_16865"
     CDS             complement(109..753)
                     /locus_tag="BVG18_RS17095"
                     /old_locus_tag="BVG18_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253921.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="WP_086045002.1"
                     /translation="MIYFNEFLLLAIIHFLAVVAPGPDFAVVVQQSISYGRKIGAITAL
                     GIGAGISVHVIYTILGFGLLIQTNAIVFNTVKIISALYLIYLGINSIKGNLKKEKSNVN
                     DSEITINNNDLTSKKSAFLKGFITNATNPKATIFFLGIFTTVVNQNTPMQVQFLYGIWM
                     CMVNAGWFILVAYLFTTKNIRNVFLKKKGLFEIFMGIVLIGLALRLLLTFN"
                     /name="LysE family transporter"
     gene            complement(818..1240)
                     /locus_tag="BVG18_RS17100"
                     /old_locus_tag="BVG18_16870"
     CDS             complement(818..1240)
                     /locus_tag="BVG18_RS17100"
                     /old_locus_tag="BVG18_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_005253919.1"
                     /translation="MNNDWLSEKITYISALKNPSDAQKLLLELAKIQYRTSDQEKKINA
                     LIKAEKAIDRANKQKVAVRKLLNAEKEAERKARTRHLIQLGALFEIAKLDQRDPAELLG
                     VLIKTAEIDPNDMKWQIWKDLGQEMLNKRSNENKKS"
                     /name="conjugal transfer protein TraD"
     gene            1521..1676
                     /locus_tag="BVG18_RS17105"
     CDS             1521..1676
                     /locus_tag="BVG18_RS17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004812002.1"
                     /translation="MQLKQVLGKIKDITFGVIAAPLIIIIMMWASILDQRAFKKERNRK
                     KEQDKT"
                     /name="hypothetical protein"
     gene            1676..2605
                     /locus_tag="BVG18_RS17110"
                     /old_locus_tag="BVG18_16875"
     CDS             1676..2605
                     /locus_tag="BVG18_RS17110"
                     /old_locus_tag="BVG18_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_086045003.1"
                     /translation="MAIARLSVKVGKAGKAAPHAEYIDRDEEKKLKQEQAETDLEHSAY
                     GNMPKWAEHNPITFWQAADLYERKNGSTYREYEIALPREMNAEQRLELVEDFIQSEIGS
                     KYPYQFAIHNPKAMDGKDQPHVHLMFNERLQDGIERDPEQYFKRYNSKNPERGGAKKDN
                     TGKSYQERKTDIKDLRQRWEDLCNSHLEKHQIDSRIDMRSYKEQGIEKEPEKKLLPSQA
                     KDPEIREALQQSRTAYKELERLDLGDPKKDLKELKDSPISDKEIKQGIESFKADFDSFK
                     QLALEQYKQQQKLEREQQKTMKFRGMSR"
                     /name="MobA/MobL family protein"
     gene            2602..3102
                     /locus_tag="BVG18_RS17115"
                     /old_locus_tag="BVG18_16880"
     CDS             2602..3102
                     /locus_tag="BVG18_RS17115"
                     /old_locus_tag="BVG18_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004282297.1"
                     /translation="MTPDEKKLYALMAVAEQQQNLVNQAVKELQITKEHIQSTIAGQTH
                     HTISKAVNEGLQDGTAELIKTAKALGRLKNSIESANDQFSWKLITTFLGVFSVLILTMT
                     WLFASYIKPLENIPKIESLRQQGIQFDIQSCAVGEESKPCVRVMKEQCGYGKHGDLCVI
                     DPK"
                     /name="hypothetical protein"
     gene            complement(3173..3924)
                     /locus_tag="BVG18_RS17120"
                     /old_locus_tag="BVG18_16885"
     CDS             complement(join(3173..3696,3696..3924))
                     /locus_tag="BVG18_RS17120"
                     /old_locus_tag="BVG18_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011789342.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WP_101627202.1"
                     /translation="MARTLLTDDIWQQIQDTMRLHGCYCSKNSRNIMEAILWKLRTGAT
                     WRDIPQEFCPWQTAYNRFNRWASKGLWDKFFFRLRGVLDQEWVFIDGSYIRVHQHASGA
                     RNGFERAIGQSRGGRTTKIHLATDANGLPIDFKITGGGVHDSQVAKQLIDTVGEATYLI
                     ADKGYDAEHIRIYAQNKDMIPIIPMRSNSKRSNKEFDKYLYKLRHLVENVFARLKHFRA
                     IATRFDKLARNYQSMIYIACIFIWCKAK"
                     /name="IS5 family transposase"
     gene            4025..4840
                     /locus_tag="BVG18_RS17125"
                     /old_locus_tag="BVG18_16890"
     CDS             4025..4840
                     /locus_tag="BVG18_RS17125"
                     /old_locus_tag="BVG18_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005268593.1"
                     /translation="MLKQSSLERTEFKEEELAPIKKLDLNDLKVPMPKNLDQLLIWTSE
                     LAQYNYAQFLNQSEQLKLVDEDNLEEQAEISQKLRFYRGNALIENQIKDFYLKKLLDQG
                     EATVKGYIKSPTKKNTRYAVISYHDYDFVSFATPEILNKFPADLINFVQKETPKTLADI
                     YPEEAVFLSAIKKFIKPFKEQYKKLADKNREIKEHNTVAHKIYIKIKNKEATLQKPTES
                     KPKESNPKTTENSAVPVEMKQGTIQKNMAPQEKPLTVIKKRKFALNKNK"
                     /name="hypothetical protein"
     Typing          1677..2602
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.469"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgaagtgcga cgctttggag gcgagttgag aagttgagag cacttgcttt tagcttttat
       61 tgtgtttagt agtcgaggta gttgttttta cttttctgct taggctattc agttgaatgt
      121 taagagaagt cgtaacgcta agcctattaa cacgataccc ataaaaattt cgaataatcc
      181 ttttttcttt aagaatacat ttctaatatt tttagtagta aataaatacg ctactaaaat
      241 aaaccatcca gcatttacca tgcacatcca aattccatat aaaaattgaa cttgcatagg
      301 ggtattttga tttacaactg ttgtaaaaat tcctaagaag aaaattgttg cttttggatt
      361 tgtagcattt gttataaatc cttttaagaa tgctgatttt tttgaagtta agtcattatt
      421 attaattgtt atttctgaat catttacgtt agatttctct tttttaagat tacctttaat
      481 tgagttaatt cctagatata ttagatataa ggcagatatt attttaactg tattaaatac
      541 aatagcattt gtttgaatca gtaatccaaa ccctaaaatt gtataaatta catggactga
      601 aattcctgct cctataccta aggctgttat tgctcctatt tttctaccgt aagaaatact
      661 ttgttgaaca actacagcga agtctggtcc cggtgctaca actgctaaaa aatgaataat
      721 tgctaaaagt aggaactcat taaaataaat cattgaagtc ttttgtcata tgaattgaac
      781 gcaatcataa attaatatgg taaacatcaa ctaaacttta agatttttta ttttcattac
      841 ttcttttatt taacatttct tgtcctaaat ctttccaaat ttgccacttc atatcatttg
      901 gatcaatttc agcagttttt ataagtactc caagcaattc agcaggatca cgctgatcta
      961 gcttagcaat ctcaaaaagt gcgcctaatt ggataaggtg gcgtgtacga gctttacgtt
     1021 cagcttcctt ttctgcattc aataattttc taacagcaac tttttgctta tttgctcgat
     1081 ctatggcttt ttcagctttg atcaaggcat taattttttt ctcttggtca gatgttctgt
     1141 actgaatttt ggctaattca agtaaaagtt tttgtgcatc actagggttc tttaaagcag
     1201 aaatataggt gattttttca gacagccaat cattattcat attcaattcc attaattcag
     1261 ataccgcaaa agtacaggct taattctcaa agaacgataa caaaataatt acgtcaaatg
     1321 tacgcaattt tgttaaagtc gttctagaga ttgttgcctg cacttccatc aaaaatttga
     1381 tggcgcgctt agacgtttct caatttcatt gagaaaccta agcacgttag ggacttcatc
     1441 cctaaaaccc tgaaacttat ttttttcgtt caattgaaaa ttgaatgaaa aaaataagta
     1501 ccaaagctag gagcaacagc atgcaactaa aacaggtatt gggaaaaatc aaagatatta
     1561 cttttggcgt gatcgcagca ccgctaatca tcatcattat gatgtgggct tcgatacttg
     1621 atcaaagagc gtttaagaaa gagcgcaacc gaaaaaagga acaggataaa acctaatggc
     1681 gattgctcgt ttaagtgtca aagtcggtaa ggcaggtaaa gcagcaccgc atgccgaata
     1741 tattgatagg gatgaagaaa agaagctgaa acaggaacag gcagaaactg atcttgaaca
     1801 tagtgcttat ggaaacatgc cgaaatgggc tgaacataat ccgattacct tttggcaggc
     1861 agccgacctt tacgagcgta aaaatggcag tacctatcga gaatatgaaa ttgccctgcc
     1921 tagagagatg aatgccgagc aacgcctaga actggttgag gacttcattc agtctgagat
     1981 cggctcaaaa tatccgtatc aatttgcgat tcataaccct aaagcaatgg acggcaagga
     2041 tcagcctcac gttcatctca tgtttaatga acgtctgcaa gatggaatcg aacgagatcc
     2101 ggagcagtat ttcaaacgct ataacagcaa aaatcctgaa cgtggcggag ctaaaaagga
     2161 caataccggt aagagttacc aggaacgaaa aactgatatt aaagacctac gccaaagatg
     2221 ggaagattta tgcaacagcc atttagaaaa acaccaaatt gatagccgta ttgatatgcg
     2281 aagctacaaa gagcaaggca tagagaaaga acctgaaaag aaactattac caagccaagc
     2341 caaagaccct gaaatcaggg aagccttaca acagtcacgt acagcctata aagagcttga
     2401 gcgactagac ttaggtgacc caaaaaaaga cctgaaagaa ctcaaagata gcccaatttc
     2461 agataaagaa attaaacagg gtattgagag ctttaaggct gattttgaca gctttaaaca
     2521 gcttgctttg gaacagtaca aacagcagca gaaattagaa agagagcaac agaaaacaat
     2581 gaagtttaga gggatgagcc gatgacacct gatgaaaaaa agctatatgc actgatggca
     2641 gttgcagaac aacagcagaa cctagtcaat caggccgtta aagagcttca aatcacgaaa
     2701 gaacacattc aaagcactat tgctgggcaa acccaccaca ctatatcaaa agcggtaaat
     2761 gagggcttac aggacggtac agcagaactt attaagaccg ctaaagcgct tggacgcttg
     2821 aaaaactcga ttgaatcagc caatgatcaa ttttcatgga agttaatcac cactttctta
     2881 ggtgtttttt cagttctcat actgactatg acgtggctat ttgcatctta tattaagcct
     2941 ttagagaata ttccaaagat cgagagttta aggcagcaag gtattcagtt tgatattcaa
     3001 agttgtgccg ttggagaaga gtccaagccc tgcgtccgag tgatgaaaga gcagtgtggc
     3061 tatgggaagc atggggattt atgcgttatt gaccccaaat agctgaatct gaaaatgaag
     3121 gctctaagca ctcatttata atttttatta tgtttaaaat tagggcgtgt cctcatttgg
     3181 ctttacacca aataaatatg caagcaatgt aaatcataga ctggtaattt cgtgcaagct
     3241 tatcaaatcg ggttgcaatc gctcggaaat gcttcaatct cgcaaacaca ttttcaacta
     3301 aatgtcttaa tttatataga tatttatcaa actccttatt cgatctttta ctatttgatc
     3361 tcattggaat gatgggaatc atatccttgt tctgggcata tattctaatg tgctcagcat
     3421 catacccctt atcagcaatg agataagttg cctcgcctac agtatcaatc agttgttttg
     3481 caacttgact gtcgtggacg ccacccccag tgattttaaa atcaatcggt aatccattcg
     3541 cgtcggttgc aagatgtatt tttgttgttc gtccaccacg tgattgtcca attgctcttt
     3601 cgaaaccatt ccgagctcca cttgcatgtt gatgcacgcg tatgtagctt ccgtcaatga
     3661 atacccattc ttgatccaag acgcctcgta atctaaaaaa aatttatccc ataatccctt
     3721 acttgcccag cgattaaagc gattgtaagc agtttgccaa gggcaaaatt cttgaggaat
     3781 atcgcgccat gtggcgcctg tgcgcagttt ccataagatg gcttccatga tatttctact
     3841 gttttttgaa cagtaacaac catgtaatcg catagtatct tgaatttgct gccagatatc
     3901 atcggtaaga agagtacgtg ccattgaaaa tatagaaata aaaataactc aaaggaggat
     3961 tttaagtatt taaaaccaat tcttcaaatg aggacacgcc ctagtggaag ttaaggaaat
     4021 tattatgtta aagcaatcaa gtttagaaag aacagagttt aaggaagaag aacttgcgcc
     4081 cattaaaaag cttgatttga atgatttgaa agttcctatg cctaaaaacc ttgatcagtt
     4141 actgatttgg acatccgaac tggctcaata taattatgct caattcctta accaatccga
     4201 acagctaaaa cttgttgatg aagacaacct ggaagaacag gcggaaatca gtcagaagct
     4261 taggttctat agaggtaatg cgcttataga aaaccaaatc aaagatttct atttgaaaaa
     4321 gttacttgat caaggagagg caacagtcaa aggttatatt aaaagtccga ctaagaaaaa
     4381 tacccgatat gctgtaattt cttatcatga ctatgatttt gtaagctttg ctactccaga
     4441 gattcttaat aaatttccag ccgatctcat caatttcgtg caaaaggaaa cacctaagac
     4501 attagcggac atatatcctg aagaagccgt gttcttatca gctatcaaaa aatttataaa
     4561 accttttaag gaacagtata aaaaattggc tgataagaac agggaaatta aggagcacaa
     4621 tacagttgct cataaaattt atataaaaat taaaaataag gaagcaactc tgcaaaagcc
     4681 aacagagtca aagcctaaag aatctaatcc aaagactact gaaaatagtg ctgtcccagt
     4741 agagatgaag cagggaacca ttcagaaaaa tatggctcca caagaaaagc cacttactgt
     4801 gattaagaag cgaaaatttg ctttaaacaa gaataagtaa cttgtaatgt aaaattctaa
     4861 acaaaaaccc ctctaagtct tgcaacttag aggggttttc aagtaaattc agcggtacgc
     4921 agttatttga tggaactaac atcaagtaac gaagatcata aggtttggcg accgttatga
     4981 tttcaccaaa aggcttgtac ctgaactcta ttttcacaat aaacagtgtt tatatcaagc
     5041 ctcaaaatga taagtcttcg ttaattcgtg ttttaaacca tcaaataagc atcctagatc
     5101 gagctttgcc cgcttctgcg tgtccgtttt acggtcggca tgcagtacgt gtggtaggaa
     5161 taagtgtcac tggcgtattg tagctatagt cgaaaggcta aaccgcttac aggggaactt
     5221 cacataggcg gagcgttggg agtagttaca taggacttgt taagttgcat gttggggaaa
     5281 ccctcgcaga gatacaggca atgtaacgac agggagcgcg tggagcgaag tgcaagactt
     5341 tgagtccttg tctgcatagt ttgcaaaact atgtgcggat acgaccgata tacgtggctc
     5401 cgaagccaaa tatatacagg taaatcggca atacttaggg gatatgtcat tttttcaaat
     5461 ggctgccctt agggtgagta ttgccgaaac tctctcaacc gtttccaaag ccaaagca
//
