LOCUS       NZ_CP019145            13816 bp    DNA     circular CON 07-APR-2024
DEFINITION  Acinetobacter lwoffii strain ZS207 plasmid pZS-3, complete sequence.
ACCESSION   NZ_CP019145
VERSION     NZ_CP019145.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06191591
            Assembly: GCF_002119785.2
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 13816)
  AUTHORS   Izak,D., Jurkowski,M., Gawor,J. and Zielenkiewicz,U.
  TITLE     Draft genome sequence of Acinetobacter lwoffii ZS207 strain,
            isolated from arsenic compounds waste
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13816)
  AUTHORS   Izak,D., Jurkowski,M., Gawor,J. and Zielenkiewicz,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2017) Microbial Biochemistry, Institute of
            Biochemistry and Biophysics PAS, Pawinskiego, Warsaw 02-106, Poland
  REMARK    Annotation added by database at submitter's request
REFERENCE   3  (bases 1 to 13816)
  AUTHORS   Izak,D., Jurkowski,M., Gawor,J. and Zielenkiewicz,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2017) Microbial Biochemistry, Institute of
            Biochemistry and Biophysics PAS, Pawinskiego, Warsaw 02-106, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 135.0x
            Sequencing Technology             :: Illumina MiSeq; 454; Sanger
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002119785.2-RS_2024_04_07
            Annotation Date                   :: 04/07/2024 16:03:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,515
            CDSs (total)                      :: 3,403
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 55 of 137
            Pseudo Genes (incomplete)         :: 83 of 137
            Pseudo Genes (internal stop)      :: 39 of 137
            Pseudo Genes (multiple problems)  :: 35 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019145.1.
            Bacteria and source DNA available from Urszula Zielenkiewicz,
            Department of Microbial Biochemistry, Institute of Biochemistry and
            Biophysics PAS, Warsaw, Poland, 02-106, Pawinskiego 5a.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13816
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="ZS207"
                     /isolation_source="microbial mats from Zloty Stok gold
                     mine"
                     /db_xref="taxon:28090"
                     /plasmid="pZS-3"
                     /geo_loc_name="Poland: lower Silesia"
                     /collection_date="2012"
     gene            join(13614..13816,1..781)
                     /locus_tag="BVG18_RS16945"
                     /old_locus_tag="BVG18_16715"
     CDS             join(13614..13816,1..781)
                     /locus_tag="BVG18_RS16945"
                     /old_locus_tag="BVG18_16715"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015224220.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_004780213.1"
                     /translation="MRTPTAISLFSGCGGMDLGFVQAGFDVLWANDLNGFACKTYAHNI
                     GNHIVHGDITEIDYAGIPSADIIIGGFPCQDFSMLWKRGGVNTDRGNLYKNFVDLVAQK
                     NPKVFVAENVKGILTANKKQAIQQIIEDFSNTGDIGYNTKAILVNFANYGVSQLRERVL
                     IIGVRKDLNQEFEVPVPTHNAENYVPSKVALEGVEKVPFNNEHQKIQPATVEKLKLIPP
                     GGNFTDIPKDSPYYVKGMISHVYRRLHPEKPSTTIIAAGGGGTWGYHYEEPRPLTNRER
                     ARLFGYPDDFEFIGSITEVRRQIGNSVPPLGVLPFAKKIKELLEKL"
                     /name="DNA cytosine methyltransferase"
     gene            778..1785
                     /locus_tag="BVG18_RS16950"
                     /old_locus_tag="BVG18_16720"
     CDS             778..1785
                     /locus_tag="BVG18_RS16950"
                     /old_locus_tag="BVG18_16720"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease PLD domain-containing
                     protein"
                     /protein_id="WP_004780212.1"
                     /translation="MMFTNLDRHHGGNFRDVLERNLIAAEKVRIASGYVSLHTIQAYST
                     QLEDIARIHGNVQLMLGMAFYEGLSIKQYDACLDLNNRLSAYSNSGVYVAHGRRYHGKI
                     YDFNQGDKNKVFVGSSNFSPSGLAGNIECTVEIVDSIQKGQVNSFLDSLFNNHSEKIDN
                     VVINTGTRKVVTLTVADKYRKLLKHARTINTSLPNVVIDLERIAEKPSSNLNVYFGKGR
                     ENMSTGKIIPRPWYEIEIISSNDINSLPNYPKGDFYAYTDDGLIIPMRTQGDNFKNLRS
                     KDSLQIFGMWLKGKLERSGSLKKYDPVTIDTLREYGNSQLKLYKISDTEYFMSF"
                     /name="restriction endonuclease PLD domain-containing
                     protein"
     gene            1980..2591
                     /locus_tag="BVG18_RS16955"
                     /old_locus_tag="BVG18_16725"
     CDS             1980..2591
                     /locus_tag="BVG18_RS16955"
                     /old_locus_tag="BVG18_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889724.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_010700378.1"
                     /translation="MIKMNTRIYLRASTKDQDAERALQILQDLNQNLNLGETIVYVENY
                     SGTKLDRPELNKLLSEANQGDTLLVESIDRLSRLTQQDFQELKRRIQEKGLRLIVADLP
                     TTYQMIQTSDSITHSILELINNMLIDLLATMARLDNEKRIERIKQGLERSGYKPTGKKA
                     NEAKHKRIKELLASGNMTKEEIAKAVNCGVATVYRVAKVI"
                     /name="recombinase family protein"
     gene            3365..3574
                     /locus_tag="BVG18_RS16960"
                     /old_locus_tag="BVG18_16735"
     CDS             3365..3574
                     /locus_tag="BVG18_RS16960"
                     /old_locus_tag="BVG18_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004282305.1"
                     /translation="MFNFKIFNKVSTEVLTIKNDLQLNAELQLINKYKTAISEDYKQAI
                     VLIFKERGYTRLEIGQLLGELKAS"
                     /name="hypothetical protein"
     gene            3826..5280
                     /locus_tag="BVG18_RS16965"
                     /old_locus_tag="BVG18_16740"
     CDS             3826..5280
                     /locus_tag="BVG18_RS16965"
                     /old_locus_tag="BVG18_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995702.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="WP_005098943.1"
                     /translation="MLSNVREQWFSNIRGDVLAGLVVGLALIPEAIAFSIIAGVDPKVG
                     LYASFCIAVVISFVGGRPAMISAATGAMALLMITLVKEHGLEYLLAATILTGVIQILAG
                     YLKLAKLMRFVSKSVVIGFVNALAILIFMAQLPELINVTWHVYALVALGLAIIYLFPLV
                     PKLGKLLPSPLVCIIAITLLAIALGIDVRTVGDMGSLPDTLPMFLIPNIPLNIETLMII
                     LPYSVALAAVGLLESMMTATIVDEMTDTPSDKYQECKGQGIANIASGFMGGMAGCAMIG
                     QSMINVKSGGLTRLSTFSAGIFLLILVVFISDWLKVIPMAALVAVMIMVSISTFEWSSV
                     TQLKDNPKSSNIVMIATVIVVVATHNLALGVLTGVLLSALFLANKLENDIQVTASFEGT
                     ARLYDLRGQIFFSSSEKFMQGFDFKEDVKDVIIDLTHSHIWDVTSVAMLDSVVKKFQKN
                     GVNVTVRGLNEASSIMIDKYGTHARI"
                     /name="SulP family inorganic anion transporter"
     gene            complement(5495..6013)
                     /locus_tag="BVG18_RS16970"
                     /old_locus_tag="BVG18_16745"
     CDS             complement(5495..6013)
                     /locus_tag="BVG18_RS16970"
                     /old_locus_tag="BVG18_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_004779644.1"
                     /translation="MKTLTVLELSKLYNINRQTIYNNIKKGILSKNSDNKIDLAEAIRV
                     FGEPNKKHDVKESVKVDSPNSAEVLLLRQQIDMLKNQLDDAKDRESFYQNQIETMQRLL
                     EAPKPHITQSGDHGSVQPEPATEAKEEVIAMPPKEPEVIENKRIPVPEHVEPEPEKRGF
                     WSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(6135..7214)
                     /locus_tag="BVG18_RS16975"
                     /old_locus_tag="BVG18_16750"
     CDS             complement(6135..7214)
                     /locus_tag="BVG18_RS16975"
                     /old_locus_tag="BVG18_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004779643.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_004779643.1"
                     /translation="MNYLTYKVGVIIIKQPPHNSNNSDSFYMAISLKKIKNFEEHDKSF
                     FPLSAPAKRYPKDFAVFQNIFWQLKHERSPKDDILKLLFLFSPKVRLASDPNETTEFTV
                     TAKEYAELTGLKLNSAYVALERVVDALYEHSVIFYHPEKDREIRTRLISTCSYKDGCFY
                     IGFTHFALYIMYVFNKQNSYTKFKVKSAVALHGHGLKLYPFLIQNEFRSNFDVSIRELK
                     SALGLAENSYPEYRDFKTTILKPHIDAINAKTELTVEFKALKKEGRKASHVNFIVRKKK
                     TIKTEQQDEIGNNQIRSKVTAKMVYLTIIQSEELSLRLKQSNETTDELVERVKNDFKNN
                     EGSRWIEKLAEFNVRFETH"
                     /name="replication initiation protein"
     gene            complement(7981..9132)
                     /locus_tag="BVG18_RS16980"
                     /old_locus_tag="BVG18_16755"
     CDS             complement(7981..9132)
                     /locus_tag="BVG18_RS16980"
                     /old_locus_tag="BVG18_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_086044969.1"
                     /translation="MTASNETIDLLTAIVDTIESSDEKAQQAKSELLEQLKLLTSKTDV
                     VSTNFPKLIETLSTFDNKIASSFSNIENIDKRLSTVTNQIKSIENSVLNTQQQSKNLQL
                     DHIIEKLSNADQQAQQLSNHLRNSSTKYLENMNAATNIARKKIEELETEVANIADSFSK
                     IVSTRIADNVVTDVSSRLKNHVFRETVAGIEIGANEVAQTLTNKAFKNIDSTLKHTEKT
                     ILSDLQLFKKESDTTHQNWIQHIKKQQAEAIAITKESHEMHMQREQDYQKHVSKEKAAV
                     KRNWIVIFLIAWFGFTATAVGIAYAVKNASESTVNEALGYIKLKDALNNFGFHPENSDY
                     CQSMLPKKQFELTAGPKVSRCFYLESPTYEYTVNSRNLMIFTK"
                     /name="hypothetical protein"
     gene            complement(9156..10382)
                     /locus_tag="BVG18_RS16985"
                     /old_locus_tag="BVG18_16760"
     CDS             complement(9156..10382)
                     /locus_tag="BVG18_RS16985"
                     /old_locus_tag="BVG18_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_086044970.1"
                     /translation="MSDKIIIRNQYSCRISAIKKSNGHSPIRAIAYIQARKMQNSLSGD
                     EHNFSNKKGVIDTGFLMPNGIETAMNEEQFYNHLENNSHASTNILAYSSIMSLPAELDI
                     NNQKKLVEDFCKYFTDTYQTAVSFAIHEPDNKKKKRAKLEEIEKHNLPSDDDDLRMQMQ
                     NNHVHLVIPYCKISALTESDLTAKRKKKAIADTFKLGNQVKDFNPIKFGSEIKKDKPDL
                     MNTQQNFLQFMRQEWCRFINNSLELNNRIERYTHKSYKDLGLQISATVHEGEARKNILA
                     SGQKTAVNEYNKRQKAKQQTEADYLNFINVPLPFELANKILSGQKLAEKELLNSLKPPT
                     QTAEDPKPGPPELREKLDRLKSTNSPGPTPGHESLQQKLDRLKQKQADPPTPLAEPKQQ
                     DNDNDYSPF"
                     /name="MobA/MobL family protein"
     gene            complement(11128..11373)
                     /locus_tag="BVG18_RS16990"
                     /old_locus_tag="BVG18_16765"
     CDS             complement(11128..11373)
                     /locus_tag="BVG18_RS16990"
                     /old_locus_tag="BVG18_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004282220.1"
                     /translation="MYSYPDSNTEKKIALMIINDFFIQKAHELWIFLQLDQCFNDYEAT
                     LIWTRRYLEKHPECEYSDIQKAFVSCFPEHFFSFDC"
                     /name="hypothetical protein"
     gene            11527..11751
                     /locus_tag="BVG18_RS16995"
                     /old_locus_tag="BVG18_16770"
     CDS             11527..11751
                     /locus_tag="BVG18_RS16995"
                     /old_locus_tag="BVG18_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004758944.1"
                     /translation="MPYTEFQRLVGKAGLSIKEFAALLDMKPNSITNYSKQGVVPTHIA
                     VIVALISTMKDEGLDFYPIFEKVKSYSQE"
                     /name="hypothetical protein"
     gene            12204..13334
                     /locus_tag="BVG18_RS17000"
                     /old_locus_tag="BVG18_16775"
     CDS             12204..13334
                     /locus_tag="BVG18_RS17000"
                     /old_locus_tag="BVG18_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004780214.1"
                     /translation="MLEPKFKLIPLTNYRHPCQVEKVSYMETGDSRFTYAVISLIPTNK
                     NIKNTKYFIRVLMMNELLKSEVEFNIASGLFTSFIHPDVSVATKGKLIPVQDSQLYESL
                     VQTAMDTFAQEYSYESIIENQDMARLFGFLISSMIGIEFNPNPVNSEVAVASELLKNLD
                     DVVYIYKNYSALNLSTELRAEINYQFWSAITMMTRSNALVYADSSKGNDFSSLADKKAD
                     LTAKNLKRAIETSLPYFNKAHIQREIRDLVAHTDFDLSNQKITCRDMIKPSHQHSKVNM
                     HGLMKYKNTIAILYRAYLTFIEIKTNELEIKKLNTVLDESIFRLSTTPQNILTIKILKD
                     LFTVVKRPLNAYGSHDVEVIFSEEIFNLIKSQALVS"
                     /name="hypothetical protein"
     Typing          complement(6136..7133)
                     /name="rep_cluster_1041"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cattggtggg tttccatgcc aagacttttc aatgttgtgg aagcgtggag gagtaaacac
       61 agatcgcggt aacttataca aaaatttcgt tgatttagta gcccagaaaa atcctaaggt
      121 ttttgttgca gaaaatgtta aggggatttt aacggcaaat aaaaaacaag caattcaaca
      181 aattattgaa gacttttcga atacaggtga tattggttat aacactaaag caattttagt
      241 taattttgcg aactatggtg tttctcaatt acgtgaacga gtcttgatta ttggtgtacg
      301 taaagatcta aatcaagagt ttgaagtacc agtaccgact cataatgctg aaaattatgt
      361 cccttctaag gtggctcttg aaggagttga aaaggtacct ttcaataacg aacatcaaaa
      421 aattcagcct gctactgttg aaaagttaaa gcttattcct ccaggtggta attttacgga
      481 tataccaaag gactcacctt actatgttaa gggaatgatt tcacacgtat atcgtcgcct
      541 acacccagaa aagccttcga caaccattat tgccgcaggt ggtggtggta cttggggcta
      601 tcattatgaa gaaccacgtc ctttaacaaa tagagagcgc gctcgtttat ttggttatcc
      661 tgatgatttt gagtttattg ggagtattac agaagttcgt cgtcaaattg gaaattctgt
      721 accaccttta ggtgtgttac cttttgctaa gaaaattaag gaattactag aaaaactatg
      781 atgtttacaa atttagatag gcatcatggc ggaaacttcc gtgatgtatt agaacgtaat
      841 ttgatagctg ctgaaaaagt tagaatcgca tcaggttatg tttcattaca taccatacag
      901 gcatatagca ctcagctaga agatattgct cgtatacatg gaaacgttca acttatgtta
      961 ggaatggctt tttatgaagg tctttctatt aagcaatatg atgcttgtct agatttgaat
     1021 aatagattat cggcatattc taattctggt gtttatgttg ctcatgggcg acgatatcac
     1081 ggaaaaattt atgattttaa tcaaggtgat aaaaataaag tttttgttgg atcatccaat
     1141 ttttcaccta gtggcttagc tggaaacatt gagtgtacag ttgagattgt tgattccata
     1201 caaaaaggac aagtgaatag ttttcttgat agtttattca ataatcattc tgaaaaaatt
     1261 gataatgtag tcataaatac tggtactaga aaagtagtca cattaacggt agctgataaa
     1321 tatagaaaat tgttaaaaca tgcgagaaca ataaacactt cactaccaaa tgtagtgatt
     1381 gatttagaaa gaattgcgga aaaaccaagt tcaaatctaa atgtgtattt cggtaagggc
     1441 agagaaaata tgagtacagg aaaaattatt cctcgtcctt ggtatgagat agaaattata
     1501 tcttctaatg atatcaatag cttgccgaac tatcctaagg gagattttta tgcttataca
     1561 gatgatgggt taattattcc aatgagaacc caaggagata atttcaagaa tttaagatct
     1621 aaagatagtc ttcaaatatt tggcatgtgg ttaaaaggga aattggaaag atctggttct
     1681 ctgaaaaagt atgatcctgt aactattgat accctaagag aatatggaaa tagccaactt
     1741 aagctttata aaattagtga tactgaatat tttatgagtt tttaacatta aagtttttct
     1801 aaaattaata taatacacgt tatacgaatt ccccaaggca tggcttttag tcatgctttt
     1861 tttatcaaaa gagtatagct gaatgataac gagccaaata acataaaata tctatcatta
     1921 aacttaattc aataattaaa tgatagtgtt atcattaatc taatagaatt aatgattaaa
     1981 tgataaaaat gaatacacgt atttatctac gagcttcaac taaagatcaa gatgcagaaa
     2041 gggctttgca gattcttcag gatctaaacc aaaacttgaa tttgggcgaa accattgtgt
     2101 acgtggaaaa ctatagtggt acgaagttag accgacctga attgaataag ctactgtcag
     2161 aagcaaatca aggtgacacc ttgttggttg aaagtattga ccgattatca cgcctaaccc
     2221 aacaggattt tcaagagctg aagcgcagga ttcaggagaa aggacttcga ttaattgtgg
     2281 cagatcttcc tactacctac caaatgattc aaaccagtga cagcattact cattcaatct
     2341 tggaactcat caacaatatg ttgattgatc tcctggcaac aatggcccgt ctagacaatg
     2401 agaaacgtat agaacgtatt aagcagggcc tggaacgttc gggttacaag ccaacaggta
     2461 agaaagccaa tgaggctaag cacaaacgaa taaaagaatt gctagcatct ggcaatatga
     2521 ctaaggaaga aattgccaaa gcagtgaatt gtggagttgc aactgtctat cgagttgcta
     2581 aagtcattta aaaaggcttg tatcagtgct tcccttatgt catatctatt tacaaaggag
     2641 caaggcattc ttaaaggtca aaagacggaa ttaatgatga aaaagcattt gattttcctt
     2701 gtttttatag tgatggtttt tattttagct ctagttgtaa agattaataa tgatcataaa
     2761 aaagggcaag cattaaagaa attggaacta gttgaaaagc aaatccatta ggtaaaaatt
     2821 gacttatact tatttaaata acgctaataa ctaagcccaa aaaacaccta gacccagtgc
     2881 tatacacgct tagtttttta tatttcaaaa gagtatttta gcaaatatca taaaagaccg
     2941 gtctattctc aagccggttt tttatatcgc agagtatcat ggcttcatca tagatatatg
     3001 aatattaaga taaataaaaa gccctatagt ctctcccgtt actataaggc ttataaaacg
     3061 ctgtaatttt ttcttaatta cctacttttc gatgtaggtt atgaatatat tttacctatt
     3121 ggtaaaaata aagatataag acaaaagatg aataccttag gaattccgcg taaaaactac
     3181 taatttcgta taaccgccat tatgttaaat agatagtact ttagtggatt attcatgctt
     3241 cccctttaga aaaacttgca gatccctttt taatttgcaa ttcttgctac caagggtgtc
     3301 tagctaagct aaggcaggtg tttgttgtag tcgggccgct acaatattga tgaagagcac
     3361 ctgtatgttt aatttcaaga tttttaataa agtctctacg gaagtattga cgatcaaaaa
     3421 tgaccttcag cttaatgctg agctgcaatt aattaataaa tataaaacgg ctatttctga
     3481 agattataaa caggctattg ttttgatctt taaagaacgt ggctatacac gtttggaaat
     3541 aggtcaatta cttggggagc taaaagcttc ataaaatacc gattaaacaa agcttggata
     3601 taacatccag gctttataaa aaagtaccta gctgttaccc ttgtcctgat ttttttttac
     3661 tctaaaaagt tccttcatct tatgacactt acgccacctt aagtttattt accgtcttaa
     3721 atctgtaaat aactcatcac tgtctagtga tgtggtgtag tcgcttgtgt gttgtatata
     3781 tcacatcact agcctatctg aaaaattaaa aaacaggcta tgtttatgtt atctaatgtc
     3841 cgagaacagt ggttctctaa catccgtggg gatgtacttg ccggattagt cgtcggtctt
     3901 gcactcatcc ctgaagccat cgcattttca attattgcag gtgtagatcc taaagtcggt
     3961 ctatatgcat ccttttgtat tgccgtcgtg atttctttcg taggtggtcg tcctgccatg
     4021 atttcagctg ccaccggggc tatggcactg cttatgatca cattggtcaa agagcatggg
     4081 cttgaatatc tgctggcagc aacaattttg acaggcgtga ttcaaatatt ggcaggctat
     4141 ttaaagcttg ccaagttaat gcggttcgta tctaaatctg tggttattgg attcgtaaat
     4201 gcgttagcga tcttaatttt tatggctcag ttgcctgaac tgattaatgt gacctggcat
     4261 gtatatgctc tagttgcttt aggtttggcg attatttatc tcttccctct ggttcccaaa
     4321 ttaggaaaat tactcccttc tccactagtc tgcatcattg caatcacatt attggcaatc
     4381 gctttaggaa ttgatgtacg gaccgtgggc gatatggggt ctttacctga cacacttcca
     4441 atgttcttga ttccaaatat tcctttgaat attgaaacat tgatgattat tttgccttat
     4501 tcagtcgcac tggcggctgt aggtttactt gaatcgatga tgacagcaac tatcgtggat
     4561 gaaatgacag atacgccaag tgataaatat caggaatgta agggacaagg gattgcgaat
     4621 attgcttctg gctttatggg tggcatggct ggctgtgcga tgattggtca atctatgatt
     4681 aacgtaaaat caggtggcct tacacgctta tcaacattct ctgcgggtat ttttcttctc
     4741 attttagttg tgtttatcag tgattggctc aaagtgattc caatggctgc attggtagcg
     4801 gtaatgatca tggtctctat cagtaccttt gaatggagtt ctgtcactca gttaaaagat
     4861 aatcctaaga gcagtaatat cgttatgatt gcgactgtga ttgtagttgt tgctacgcat
     4921 aacctggctt tgggtgtgtt aacaggcgta ttactttcag ccttgttcct ggcgaataaa
     4981 cttgaaaatg atatccaggt aacagcatct tttgaaggaa cggcccgttt atatgatttg
     5041 agaggtcaga tcttctttag ttcttctgaa aaattcatgc aaggttttga ctttaaagag
     5101 gatgtaaagg atgtgattat tgatttgacc cattctcaca tttgggatgt aacatcagtc
     5161 gccatgcttg attctgttgt aaaaaaattt caaaagaatg gtgtgaatgt cactgtgcgt
     5221 ggcttaaatg aagccagctc tatcatgatt gataagtatg gaacacatgc cagaatctaa
     5281 gcgcagaaga ttttacgttt acgtagaatc tctaaaatta acataataca ccttatacga
     5341 aatgcgttat aattccactt agaaatcaat gctgtagttt ctagttgata gcccatcttc
     5401 cccaaggtgg gtttttttta tgatttttca cgttccacgc ctattctttg accaaagttt
     5461 cacgactttg ttcatattag ttaaacaatc gtggttaatc atagggtcta aaaaaacggc
     5521 tccaaaaccc tcttttttcc ggttcaggct ctacgtgttc aggaactgga atgcgcttgt
     5581 tctcgattac ttcaggttct ttaggtggca ttgctataac ctcttctttt gcttctgtcg
     5641 ctggttctgg ctgtaccgat ccatgatcac cagattgagt gatatggggt tttggtgctt
     5701 ccaatagacg ttgcatcgtt tcaatttgat tctgataaaa agactcgcgg tctttggcat
     5761 catcaagctg atttttcagc atgtctatct gctgtctaag cagtaaaact tctgccgaat
     5821 ttggactgtc tacttttaca gactctttta catcatgttt tttattaggc tccccaaata
     5881 cacggatagc ttcggctaga tcaatcttat tatcagaatt tttgcttaaa attcctttct
     5941 ttatgttgtt gtaaatagtt tgtctattaa tattgtaaag cttggaaagt tcaagaaccg
     6001 taagggtttt catcctgtaa acctatgcct aaacttgtaa aaagttagtg tctattagac
     6061 tgtctaatag acaaatgatc aagtttaaat tttaagcaag gtaaatgata atttttaaaa
     6121 taaattatga tgttttagtg agtttcaaac cgcacattaa attccgctaa tttttctatc
     6181 catctgctac cttcattatt cttaaaatca ttttttactc gttcgactaa ttcatctgtt
     6241 gtctcattgg attgtttaag ccgtaaactt aattcttcac tttgaattat cgttaagtaa
     6301 accatttttg cagtgacttt agatcgtatt tggttattac cgatttcatc ttgttgttct
     6361 gtttttatag tcttcttttt tctcactata aaattaacat gactagcttt acgtccctct
     6421 tttttaagag ctttaaattc aacagtaagt tctgttttcg cgttaattgc atcaatgtga
     6481 ggtttaagaa tagtagtttt aaaatctcgg tactcagggt aggaattttc agcaaggcct
     6541 aaagcacttt ttagttccct aatggaaaca tcaaaattag agcgaaattc attttgtatt
     6601 aggaatggat agagttttaa gccatggcca tgtaaggcaa ctgctgattt cactttgaat
     6661 tttgtatacg aattttgctt gttaaaaaca tacataatgt atagggcaaa gtgcgtgaaa
     6721 ccaatataaa agcatccatc cttatatgag catgtactaa tgagtcttgt acgaatttct
     6781 ctatcttttt caggatgata aaaaataaca ctatgttcat aaagagcatc tacgactctc
     6841 tctaatgcta cataagcact gtttagcttt aatcctgtta attccgcata ttccttagct
     6901 gtcacagtaa attcagttgt ttcatttggg tctgatgcta gtcgaacttt tgggctgaat
     6961 aagaacagta attttaaaat atcatcttta ggagaccgct catgttttaa ttgccagaaa
     7021 atattttgaa agacagcaaa atcttttggg taacgtttag ctggtgctga aagaggaaaa
     7081 aaagatttgt catgttcctc aaaattctta attttcttta atgatatagc catgtagaag
     7141 gagtcactat tattactatt atgtgggggt tgcttgataa taattacccc cactttataa
     7201 gtcaagtaat tcatataacc taacaatcca cccccaccaa cctaacaatc cacccccacc
     7261 aacctaacaa tccaccccca ccaacctaac aatccacccc cactttaagc caaaaaccta
     7321 acaatccacc cccactttaa gccaaaaacc taacaatcca cccccacttt gatgtctaaa
     7381 agccttatct atcaatacat tcatactatc cgtaaagctt tttaaaagct ttttaaaagc
     7441 tattaaaagc ttttaataaa gcttttaaaa taaatctaaa taaaaattgc ccttggtttt
     7501 cgctatgctc aaactctatt gaatctcgct ttcgctcgat tctgtggggc aattttttgc
     7561 ttaattcagt tgtaaattaa aggaaaaaca aaagctccat cgttcgcgca cttcgtgacg
     7621 ctcttcatct cccgtttaga ccaataatgg gagcctgtaa tcagggaaaa agtggtggca
     7681 aaattcactt acaaccgttc gctcgtagac actcgctctg taagttattg ttattattta
     7741 aatattctta ataacggtga gattgatagt aatttattta attgaaaagt attttttaat
     7801 ttattaacaa catcacttaa aacacaagat gaatctatct ctatttcttc accatttcag
     7861 ccacattgaa tttttattgt tttttggtat caatgtaccg attttattgt ttcgttcaat
     7921 ctgagcgatt ctgtgcgttt ttagagatcc aaaaataagc cctcaatcga gggcctaaat
     7981 ttattttgtg aaaatcatta gatttcgact gttcacagta tattcatagg ttggactttc
     8041 taaataaaaa cacctagata cttttggacc agcagttaac tcaaattgtt tcttcggcaa
     8101 catactttga caataatcac tattttcagg atgaaaaccg aaattgttga gagcatcttt
     8161 caatttgata tagccaagag cttcgtttac tgttgattca gatgcatttt ttactgcata
     8221 agcaatacca acggcagttg cagtaaaacc gaaccaagca ataaggaaaa ttacaatcca
     8281 atttctcttt acagcggctt tttctttgct gacatgcttt tgataatcct gttctcgttg
     8341 catgtgcatt tcgtgtgatt ctttcgttat tgctattgct tctgcttgtt gttttttgat
     8401 gtgttgtatc caattttgat gtgttgtatc actttctttt ttgaataact ggaggtcgct
     8461 taagattgtt ttttcagtgt gtttgagagt tgaatcaatg tttttaaacg ctttgtttgt
     8521 aagtgtttga gctacttcgt tcgctcctat ttcaatgcct gctacggttt ctctgaaaac
     8581 atgattcttc aatcgtgatg aaacatctgt gacgacatta tctgcaattc ttgttgaaac
     8641 gatttttgaa aaactatctg caatgtttgc tacttctgtt tctaattctt cgattttttt
     8701 tcgtgcaatg tttgttgctg cattcatatt ttctaaatat ttcgtgcttg aattacgtaa
     8761 gtgattgctt agctgttgtg cttgttgatc tgcattactg agcttttcga tgatgtgatc
     8821 tagttgtaaa ttttttgatt gttgctgagt attaagaact gagttttcta ttgatttgat
     8881 ttgattagtg actgtactta aacgcttgtc gatgttctcg atattgctga aactcgatgc
     8941 aatcttgttg tcgaaagttg atagcgtttc gatcaatttt ggaaaatttg tgctgactac
     9001 atctgttttt gatgtaagca atttcaattg ttcgagtagc tctgattttg cttgctgtgc
     9061 tttttcatct gagctttcaa tcgtgtcgac aattgcagtc aaaagatcaa tagtttcgtt
     9121 acttgcagtc atgtgacttc cttatttatt aataattaaa agggtgagta atcgttgtcg
     9181 ttgtcttgtt gcttcggctc ggccagggga gttggtggat cagcttgctt ttgctttaaa
     9241 cgatctaatt tttgttgcaa tgattcgtgt ccgggtgtgg gaccagggga gttggtggat
     9301 tttagacgat ctaatttttc acgcagctca ggtgggccag gctttggatc ttctgccgtt
     9361 tgggtcggtg gtttgagact gtttaataat tctttttcag caagtttttg accgctcaag
     9421 attttatttg ctaactcgaa aggcaaaggc acattgataa aattgagata atccgcctct
     9481 gtttgttgct ttgctttttg acgtttgttg tattcattca cagccgtttt ttgaccgctt
     9541 gctagaatgt tttttcgtgc ttcaccttcg tgaacagtgg ctgaaatctg caatccgagg
     9601 tctttatatg atttatgtgt atagcgttca attctgttgt ttagctctaa cgaattatta
     9661 ataaatctgc accactcttg acgcatgaat tgcaaaaaat tttgctgtgt attcatcaaa
     9721 tcaggtttgt cttttttgat ctctgagccg aatttgattg gattaaaatc tttcacttgg
     9781 ttgccgagct tgaacgtgtc agcaatcgct tttttcttac gctttgctgt taagtcgctt
     9841 tctgtgagtg ctgaaatttt gcagtacgga atcactaaat gaacatgatt gttctgcatt
     9901 tgcatacgca aatcgtcatc atcagacggc aaattatgct tttcgatttc ctctagtttt
     9961 gcccgtttct tttttttgtt gtctggttcg tgaatcgcaa aactaactgc tgtttgatat
    10021 gtgtctgtga aatatttgca aaaatcttcg actaattttt tttgattatt tatgtctaat
    10081 tccgcaggca atgacatgat tgacgaatat gcaagaatgt ttgttgatgc gtgagagttg
    10141 ttctctaaat gattataaaa ttgttcttcg ttcatagctg tttcaatgcc gttaggcatt
    10201 aaaaaacctg tgtcgatcac gcctttttta ttagaaaaat tatgctcgtc accactcaaa
    10261 ctgttttgca tttttcgtgc ttgaatatat gcaattgctc gaattgggct atgaccattt
    10321 gattttttaa ttgcagaaat tcgacaagaa tattgattac gaataatgat tttatcagac
    10381 atgcttttgc gcttttgctt tgtactttta acacgttagc tgacataaaa aaattggcaa
    10441 agcacaatga tttttagatc agaaaaaagg tttaaaagtc cccccccttc cctggttttt
    10501 taggggggcg cacgactcaa aacgaacatg agcgaagcga aatgtgcgtt ttgggttaaa
    10561 gtgcgcttca ttctacgtca tttctacgtt tttacagtct acgcttgcgc ttgactgtaa
    10621 aaacgtgaaa tgtcgtgaat gaagttgtca cgtaattcga ctatacacta caaaaaaaac
    10681 tgacctgtat ttggtgttat ttttcgattt ttttcacttg tgataatttc aattaattgt
    10741 tttttccgtg atagtaatat gaatattgat tactctaaat gtgtcttgat gaatactaag
    10801 acaaaatctt ttttacagta tcatttcaat aatgagaaag tgaaagactt agggactcaa
    10861 atcaaatttt atgcatcatt taactgactt atcgtatttt ataagttttt atttttgtat
    10921 ctttaaagta caccctaaag taacgtcatg gcggccccaa tcctgaacct aaaatcaatg
    10981 tcttagatgc cgtggaacat caattttagt atgctcaaag aaagtcttag atttatacgt
    11041 gacaataaaa agccagttgt atgaactggc tttttctatt tgggatttac ttacccattg
    11101 cgattttttt accgtgcctc tcataaatta acagtcaaag ctgaagaagt gttctgggaa
    11161 acaagaaaca aaggcttttt gaatatcgct gtactcacac tcaggatgct tctctaaata
    11221 acgtcgagtc caaatcagtg tggcttcata atcgttaaag cactgatcga gttgtaaaaa
    11281 aatccacagt tcatgggctt tttgaatgaa aaagtcattg atgatcatga gagcaatttt
    11341 cttttcagtg tttgaatccg ggtatgagta catattacat ttctccgata gtaaggataa
    11401 ataggttagt tgataagcta tttataacac ataaaaatat gtgtttacca cataatttta
    11461 tgtgttttta tgtaaatata accctatgct ataatctcaa aacaccattt ctataaattt
    11521 aattaaatgc catatactga atttcaacgt cttgtcggta aagcagggct ttcaattaag
    11581 gaatttgctg ccttattgga tatgaaacct aattcgatta cgaattacag taaacaaggt
    11641 gtggttccta cacatattgc tgtaatcgtt gcgttgattt ctacgatgaa agatgaggga
    11701 ttagatttct atccaatttt tgaaaaagtt aagtcgtatt cacaagaata aaactctcgt
    11761 tttttgattg ttgtaagctt tttagtagat attgatgatg taaaaaatgc ctgaacttca
    11821 ggcatttttt tgtttcaggg acttataagc ctcgatagaa cttttcaata aagtatcgag
    11881 ttttgagaac tagggtgatc atcaaactct tatgttctga gatcttaggt cgagcaatcg
    11941 gtaaaccgga catttctatt tgggggttac aactgaattt cgcataagaa attttatgtt
    12001 aaagaagagt caaagatacc tagaaaatta aagtcataat tatttcgcca aatacaaata
    12061 agttatagat tgtgaatact aatattcttc ttgttctgga aaaatgttgc gtcagtgtgc
    12121 ttaaagactt gataattcat caacttgata tctacatcat ctagatatgt attatcattc
    12181 cttagagtcc tttaggaatc aaaatgttag agccaaaatt taagttaata cctcttacta
    12241 attatcgtca tccttgtcag gttgaaaaag tctcttacat ggaaacgggt gattcaagat
    12301 ttacctacgc tgtgattagc ctgataccca ctaataagaa tattaaaaat acaaaatact
    12361 ttatccgtgt ccttatgatg aatgagctgc ttaaatctga ggttgaattt aatattgcat
    12421 ccggcttatt caccagtttc atacatcctg atgtttctgt tgcaactaaa ggcaaattga
    12481 ttccagtcca agattcacag ttatacgaat cattggttca aactgctatg gatacttttg
    12541 ctcaggaata tagctatgaa tccattattg aaaaccagga tatggctcgt ctgttcggat
    12601 ttttaatatc ttcaatgata ggaattgagt ttaatcctaa tcctgttaat tctgaggtag
    12661 ctgttgcttc ggaactttta aaaaatcttg atgatgttgt ctatatttat aaaaactact
    12721 cagctctcaa tcttagtact gagcttagag ctgaaataaa ttatcaattc tggtcagcca
    12781 ttacgatgat gacccgtagt aatgccttgg tatatgcaga tagttcgaaa ggcaatgatt
    12841 tctcaagcct ggcagataag aaggctgatc taactgctaa aaatcttaaa agagcaattg
    12901 agaccagcct cccttacttt aataaagcac atattcaacg tgaaattcga gatttagttg
    12961 cacatacgga ttttgactta tctaaccaaa agatcacatg cagagacatg attaagcctt
    13021 cacaccaaca tagtaaagtg aatatgcatg gattaatgaa atataaaaat acaatagcaa
    13081 ttctttaccg tgcctatctt acttttatag agataaagac caatgaactt gagattaaaa
    13141 agctaaatac tgttttagat gaatccattt ttcgtctttc aaccacccct caaaatattc
    13201 ttacgattaa aatacttaaa gatctgttta ctgtagtgaa aagaccttta aatgcttatg
    13261 gaagtcatga tgttgaggtt atttttagtg aagaaatttt taatttaatt aagagtcaag
    13321 cacttgtatc ttgatcaaca taaaatcttt ttgaaggata tcaagcgtat gatattccta
    13381 cgctttttta attcctataa gagtttttat gttaaatgaa gtcttagggt taatatgtga
    13441 aagaacattg ttctggatca acaatttcat tgtcttatga ataagggagc attaaaagct
    13501 cccttattca tttctgttta cgacataact tttcgcatag atgtataaca ttaatcaact
    13561 ttctgtgttc tacggtgtat tatcaacgct atttttattg ttgttgattt tatatgagaa
    13621 ctccgaccgc tatttctcta ttctctggtt gtggaggaat ggatttaggc ttcgtccaag
    13681 ctggttttga tgtcttatgg gcaaatgatt taaacgggtt tgcttgtaaa acttatgctc
    13741 ataatattgg taatcatatt gtacatggtg atattactga aattgattat gcaggaattc
    13801 cgagcgcaga tatcat
//
