LOCUS       NZ_CP019025             6647 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_881 plasmid pEC881_4, complete
            sequence.
ACCESSION   NZ_CP019025
VERSION     NZ_CP019025.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511150
            Assembly: GCF_002012225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Stoesser et al,N.
  TITLE     Unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002012225.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:39:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,524
            CDSs (total)                      :: 5,400
            Genes (coding)                    :: 5,174
            CDSs (with protein)               :: 5,174
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 83 of 226
            Pseudo Genes (incomplete)         :: 157 of 226
            Pseudo Genes (internal stop)      :: 44 of 226
            Pseudo Genes (multiple problems)  :: 51 of 226
            Pseudo Genes (short protein)      :: 1 of 226
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019025.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_881"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC881_4"
                     /geo_loc_name="Colombia: Cali"
                     /lat_lon="3.45 N 76.54 W"
                     /collection_date="2013"
                     /collected_by="Merck Study for Monitoring of Antimicrobial
                     Resistance Trends (SMART)"
     gene            complement(139..1707)
                     /gene="cea"
                     /locus_tag="BE930_RS00090"
                     /old_locus_tag="BE930_00090"
     CDS             complement(139..1707)
                     /gene="cea"
                     /locus_tag="BE930_RS00090"
                     /old_locus_tag="BE930_00090"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            1967..2404
                     /locus_tag="BE930_RS00095"
                     /old_locus_tag="BE930_00095"
     CDS             1967..2404
                     /locus_tag="BE930_RS00095"
                     /old_locus_tag="BE930_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            2485..2904
                     /locus_tag="BE930_RS00100"
                     /old_locus_tag="BE930_00100"
     CDS             2485..2904
                     /locus_tag="BE930_RS00100"
                     /old_locus_tag="BE930_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(3222..3455)
                     /locus_tag="BE930_RS29190"
     CDS             complement(3222..3455)
                     /locus_tag="BE930_RS29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3462..3980)
                     /locus_tag="BE930_RS29195"
     CDS             complement(3462..3980)
                     /locus_tag="BE930_RS29195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4018..4716)
                     /locus_tag="BE930_RS30360"
                     /pseudo=""
     CDS             complement(<4018..4716)
                     /locus_tag="BE930_RS30360"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4658..4981)
                     /locus_tag="BE930_RS00120"
                     /old_locus_tag="BE930_00120"
     CDS             complement(4658..4981)
                     /locus_tag="BE930_RS00120"
                     /old_locus_tag="BE930_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5045..5236
                     /locus_tag="BE930_RS00125"
                     /old_locus_tag="BE930_00125"
     CDS             5045..5236
                     /locus_tag="BE930_RS00125"
                     /old_locus_tag="BE930_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5906..6064
                     /locus_tag="BE930_RS30365"
     CDS             5906..6064
                     /locus_tag="BE930_RS30365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(6263..6400)
                     /locus_tag="BE930_RS00140"
                     /old_locus_tag="BE930_00140"
     CDS             complement(6263..6400)
                     /locus_tag="BE930_RS00140"
                     /old_locus_tag="BE930_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     Typing          complement(5322..5409)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          5420..5978
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.544"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3119..4668)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agattttttc gaaagaagaa aaaacctaaa taacgccccc gtagcaaaat taaacctatt
       61 tatgctatat aatctacttt gtttggtcct agcaatccca tttggattgc taggactttt
      121 tatatcaata aagaataatt aaatccctaa cacctcattt atagtattaa gtttattctt
      181 atcaatatag gagcatagaa ttcctgtaac aatagcaata ccccaaatac ctaatgtagt
      241 tccagcaagc aagctaaaaa gtaaagcaac aacataactc acccctgcat ctgcagcttt
      301 cttctctaat gtaagaaata gtggcttcca gtcacctgta tccttaattt ttaggatatc
      361 agataccaca tcatatccaa aagaaacatg ccccgtaatc ttcaagtact tggcaaactg
      421 atctaaatgt ttagcccagt catcatactt caccgatgcc aacgcattaa aaatagcatc
      481 acgatcggct ttgctgaatt tcttatttaa aacatccttg tatttttcaa aagcagcgag
      541 agcttcattc acattgccga ttttcttacc tttagactta tcagcaagtt cctgtgccat
      601 tttcgaatat ttttcaccat atttttcagt cagcgtttga taaaagctaa ctgttgcatc
      661 aacagcatcc ttaatctgtg aattaaggag attattctgt gcttttttca aattttcttc
      721 agcttcatga acacgagcga taccggcatt acgattatta ctgacctgag aaatagcctt
      781 ctggatctga gttatatcag catttatccg gttaatacgt gtttctgatg ctgttacctg
      841 tttttgtttt tcttctctaa tcttaccggc cccaacccgt cgtctggttg cttcaaaaaa
      901 aggacggttc tgaagcggat cattggctct tggtgatagt tttttgacca gctcatccag
      961 ttctttatat ttagcggatg cctgagccag ttcatttcgt tttccagcga gcgttttcat
     1021 ttctgcatca cgggcatgga tactggagct taaacgagaa ttgagagtct taatctctcc
     1081 atccattttc accacttcag attgtgcagc agaaagtttt ttttgggcga tctcaacagc
     1141 tttagcttct tcactcaatg cagccagtcg tttctcttca gcttcagcca gtttcaactg
     1201 gcgttctgtt tcagccttct cccgttcaat ctctttacgt cgttgttctg cttcctgaaa
     1261 agccttttct gctgcttccg cttctttacg ggctttttct tctgctttcg caaggcgcaa
     1321 acgctctgct tccgcctgca tagctgcatt attagcatga gcaagctctg ttgctgaagg
     1381 cgtacgtgag gcattgtgac gaagagcctc attcacgata tccttcaggc gctgagtcag
     1441 cgcatccctg tttgcctttg ctttcgcctg tgcttccgct gcagcttttg cccgggcagc
     1501 ctgctctgcc tgtgttttct ttaattgagc agtagaccat ttagcagttg catgaatagc
     1561 tgcagaactt tcacttttac tgcctccttt tccacctccg ccgccagagc cactcccgtc
     1621 aggagtacca ttcaaaagag taataattac ctgtccctta tcatcataag gaacaccatc
     1681 tttatagtac gctaccgcgg tttccattat aaaatcctct ttgactttta aaacaataag
     1741 ttaaaaataa atactgtaca tataaccact ggttttatat acagcataaa agctacgccg
     1801 ctgcattttc cctgtcaaga ctgtggactt ccatttttgt gaaaacgatc aaaaaaacag
     1861 tctttcacac cacgcgctat tctcgcccga tgccacaaaa accagcacaa acattaccgt
     1921 tctcagacct cattatgttt tactgaaact atgagatgag acatctatgg gacactgtca
     1981 ctttatggca tggcacacac tccgggacgc actaaaaatg acaggcagat cgcgttcaca
     2041 gttttaccgt gatatgcgcg caggccttgt cagttaccgt accggcaggg acggacgacg
     2101 ggagtttgaa accagtgaac tgatccgggc atacggcgaa ttaaagcaga atgagacacc
     2161 agaaaggcac agtgagggac atgcagaaaa tccacatgat cagcagacag aacgcattct
     2221 ccgggaactg aatgagctga aacaatgcct gacgctgatg cttgaggata aacaggcaca
     2281 ggatatggat cgcagacgcc aggaagcaga acgggaacag ctacaaaatg agatagccca
     2341 gctcaggcag gcactggaac tggaaaagaa acggggattc tggtccaggt tgttcggtcg
     2401 ctgaacgctg tcagagactg atgataaaat agtcttcgga taataactca ccgagaataa
     2461 atactttaag gtagggagac actcatgaga cgtaccggaa acaaactttg tcttatcgcc
     2521 atgataacag caacagtagc tctcacagcc tgtaccccaa agggcagcgt ggaacaacat
     2581 acccggcatt acgtatatgc ttctgatgac ggttttgatc ccaacttttc cacccaaaaa
     2641 gccgacacaa cacgaatgat ggtgcctttt tttcggcagt tctgggatat gggagctaaa
     2701 gataaagcga caggaaaatc acggagtgat gtgcaacaac gcattcagca gtttcacagc
     2761 caggaatttt taaactcact ccggggcaca actcaatttg cgggtactga ttaccgcagc
     2821 aaagacctta ccccgaaaaa atccaggctg ctggctgaca cgatttctgc ggtttatctc
     2881 gatggctacg agggcagaca gtaagtggat ttaccataat cccttaattg tacgcaccgc
     2941 taaaacgcgt tcagcgcgat cacggcagca gacaggtaaa aatggcaaca aaccacccga
     3001 aaaactgccg cgatcgcgcc tgataaattt taaccgtatg aatacctatg caaccagagg
     3061 gtacaggcca cattaccccc acttaatcca ctgaagctgc catttttcat ggtttcacca
     3121 tcccagcgaa gggccatcca gcgtgcgttc ctgtatttcc ggctgacgct cccgttccag
     3181 ggataacaca tgttcgcgct cctgtatcag ccgttcctct cttatctcca gttctcgctg
     3241 tataactggc tcaagcgttc tgtctgctcg ctcaagtgct gcacctgctg actcaactgc
     3301 atgacccgct cgttcagcat cgcgttgtcc cgttgcgtaa gcgaaaacat cttctgcaat
     3361 tccacgaagg cgctctccca ttcgctcagc cgctgcatat agtcctgttg cagctgctct
     3421 aaggcgttca gcaaatgtgt ttccagctct gtcactctgt gtcactcctt cagatgtacc
     3481 cactctttcc cctgaaaggg aatcacctcc gctgatttcc cgtacggaag gacaaggaat
     3541 ttcctgttcc cgtcctgcac aaactccacg ccccatgtct tcgcgttcag tttctgcaat
     3601 gtctcttcct gcttcctgat ttcttccagg ttcgcctgta tcctccctcc aagataccag
     3661 agcgtcccgc cactcgcggt aaacaggaga aagactatcc ccagtaacat catgcccgta
     3721 ttccctgcca gctttaacac gtccctcctg tgctgcatca tcgcctcttt caccccttcc
     3781 cggtgttttt ccagcgattc ctctgtcgag gctgtgaaca gggctatagc gtctctgatt
     3841 ttcgtctcgt ttgatgtcac agcctcgctt acagattcgc cgagcctcct gaactcgttg
     3901 ttcagcattt tctctgtaga ttcggctctc tctttcagct ttttctcgaa ctccgcgccc
     3961 gtctgcaaaa gattgctcat aaaatgctcc tttcagcctg atattcttcc cgccgttcgg
     4021 atctgcaatg ctgatactgc ttcgcgtcac cctgaccact tccagccccg cctcagtgag
     4081 cgcctgaatc acatcctgac ggccttttat ctctccggca tggtaaagtg catctatacc
     4141 tcgcgtgacg ccctcagcaa gcgcctgttt cgtttcaggc aggttatcag ggagtgtcag
     4201 cgtcctgcgg ttctccgggg cgttcgggtc atgcagcccg taatggtgat ttaccagcgt
     4261 ctgccaggca tcaattctag gcctgtctgc gcggtcgtag tacggctgga ggcgttttcc
     4321 ggtctgtagc tccatgttcg gaatgacaaa attcagctca agccgtccct tgtcctggtg
     4381 ctccacccac aggatgctgt actgattttt ttcgagaccg ggcatcagta cacgctcaaa
     4441 gctcgccatc actttttcac gtcctcccgg cggcagctcc ttctccgcga acgacagaac
     4501 accggacgtg tatttcttcg caaatggcgt ggcatcgatg agttcccgga cttcttccgg
     4561 attaccctga agcaccgttg cgccttcgcg gttacgctcc ctccccagca ggtaatcaac
     4621 cggaccactg ccaccacctt ttcccctggc atgaaattta actatcatcc cgcgccccct
     4681 gttccctgac agccagacgc agccggcgca gctcatcccc gatggccatc agtgcggcca
     4741 ccacctgaac ccggtcaccg gaagaccact gcccgctgtt caccttacgg gctgtctgat
     4801 tcaggttatt tccgatggcg gccagctgac gcagtaacgg cggtgccagt gtcggcagtt
     4861 ttccggaacg ggcaaccggc tcccccaggc agacccgccg catccatacc gccagttgtt
     4921 taccctcaca gcgttcaagt aaccgggcat gttcatcatc agtaacccgt attgtgagca
     4981 tcctctcgcg tttcatcggt atcattaccc catgaacaga aatccccctt acacggaggc
     5041 atcagtgact aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac
     5101 attaacgctg ctggagaagc tcaacgaact ggacgcagat gaacaggccg atatttgtga
     5161 atcgcttcac gaccacgccg atgagcttta ccgcagctgc ctcgcacgtt tcggggatga
     5221 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtgagcgga
     5281 tgccgggagc tgacaagccc gtcagggcgc gtcagcaggt tttagcgggt gtcggggcgc
     5341 agccctgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacca tgcggcatca
     5401 gtgcggattg tatgaaaagt acgccatgcc gggtgtgaaa tgccgcacag atgcgtaagg
     5461 agaaaatgca cgtccaggcg cttttccgct tcctcgctca ctgactcgct acgctcggtc
     5521 gttcgactgc ggcgagcggt actgactcac acaaaaacgg taacacagtt atccacagaa
     5581 tcaggggata aggccggaaa gaacatgtga gcaaaagacc aggaacagga agaaggccac
     5641 gtagcaggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atagacgctc
     5701 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5761 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5821 cccttcggga agcgtggcgc tttctcatag ctcacgctgt tggtatctca gttcggtgta
     5881 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5941 cttatccggt aactatcgtc ttgagtccaa cccggtaagg cacgccttaa cgccactggc
     6001 agcagccact ggtaaccgga ttagcagagc gatgatggca caaacggtgc tacagagttc
     6061 ttgaagtagt ggcccgacta cggctacact agaaggacag tatttggtat ctgcgctctg
     6121 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6181 gttggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6241 caagaagatc ctttaatctt ttctactgaa ccgcgatccc cgtcagttta gaagaggagg
     6301 atggtgcgat ggtccctccc tgaacatcac gtatatagtt agcctgacat ccaacaagga
     6361 ggtttatcgc gaatattccc acaaaaaatc ttttcctcat aactcgatcc ttataaaatg
     6421 aaaagaatat atggcgaggt ttaatttatg agcttaagat actacataaa aaatatttta
     6481 tttggcctgt actgcacact tatatatata taccttataa caaaaaacag cgaagggtat
     6541 tatttccttg tgtcagataa gatgctatat gcaatagtga taagcactat tctatgtcca
     6601 tattcaaaat atgctattga atacatagct tttaacttca taaagaa
//
