LOCUS       NZ_CP019024             4063 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_881 plasmid pEC881_5, complete
            sequence.
ACCESSION   NZ_CP019024
VERSION     NZ_CP019024.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511150
            Assembly: GCF_002012225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Stoesser et al,N.
  TITLE     Unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002012225.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:39:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,524
            CDSs (total)                      :: 5,400
            Genes (coding)                    :: 5,174
            CDSs (with protein)               :: 5,174
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 83 of 226
            Pseudo Genes (incomplete)         :: 157 of 226
            Pseudo Genes (internal stop)      :: 44 of 226
            Pseudo Genes (multiple problems)  :: 51 of 226
            Pseudo Genes (short protein)      :: 1 of 226
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019024.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_881"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC881_5"
                     /geo_loc_name="Colombia: Cali"
                     /lat_lon="3.45 N 76.54 W"
                     /collection_date="2013"
                     /collected_by="Merck Study for Monitoring of Antimicrobial
                     Resistance Trends (SMART)"
     gene            join(3501..4063,1..139)
                     /locus_tag="BE930_RS00065"
                     /old_locus_tag="BE930_00065"
     CDS             join(3501..4063,1..139)
                     /locus_tag="BE930_RS00065"
                     /old_locus_tag="BE930_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(387..2534)
                     /gene="mobQ"
                     /locus_tag="BE930_RS00070"
                     /old_locus_tag="BE930_00070"
     CDS             complement(387..2534)
                     /gene="mobQ"
                     /locus_tag="BE930_RS00070"
                     /old_locus_tag="BE930_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            2713..2967
                     /gene="mobC"
                     /locus_tag="BE930_RS00075"
                     /old_locus_tag="BE930_00075"
     CDS             2713..2967
                     /gene="mobC"
                     /locus_tag="BE930_RS00075"
                     /old_locus_tag="BE930_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(2177..2535)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2536..2712
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3502..4063
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(391..2621)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.989"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gataatcaag ccagccatcg ccgaactgaa cgaaaaaagc gatctggttg taactgttga
       61 gacgatcaaa aaggggcgca cggttgtagc cctgcatttt cgcttcaagg aagacaaaca
      121 gatcaagatg acgatctaaa atcgatttta aggcacctaa aacgctccct gctgcgtttt
      181 ttttgtgtta gtagtgcgaa ggtactattg aagtgaaaaa ttgcgtcaaa aacgctctga
      241 tgcagcgaaa aaagaccccc caatttttgg gggcggaaaa acaaaactat gcttgcgtta
      301 tgtgagtgtg cttcttgcat tcatccctgt cgatgaacat cgacgaacat aacacgatgt
      361 taaacaaaac aaaatattgt tcacattcac tgtgaccaac cgcgatcatg tctcctctcg
      421 gtggtgtgtg ttcgtgcatc ttcctcgcgc tgccattcct cccaggtacg attaaaaccc
      481 agcgagcgga acagatgatc cggctgtttt tctggtgtgt tgacggcagc aaaaagcggt
      541 gcctcacggg ttttcagatg aaactgcatc agttctggtg caggtgatat gtggctgtct
      601 gcatcgtgat ttacggcaac accataccct gcataccgta accccttatc ggtcagcgca
      661 ctgatgacca gatgcctgtt atcggctgag ggaacggcaa acgcgggata cagcgtacca
      721 ttaagccgga taacaccgcc cagagagtca tccacgccca tgaccagcaa aagctgaagc
      781 tggcgggttt cccggtcgac agaactcatg accggcatca gcgtcggtgg cagacgtccc
      841 tgcataaaat ccaccttcct tgccgctggt gatggcatgg ctggctggcg gtcttctcca
      901 agacagaaag actccggcca tgtcttcggc tggcggagat tgcgggtatg ttgcggccat
      961 gtctggcaaa cctgcgtcag cagttgctca aaggttgcag aggaataaac acgttgcagt
     1021 ggcggacgtt ggccgatttc gcttcggtca cagatataac cactcctgtc acgcattaaa
     1081 tcaacactct caaagattct ggtgcggtat ttctccagct cgttccccca gagcgtatat
     1141 tctccgttcc ggttgtgaag cagaaggtaa caggtcattt ccccgttacc aggcatggga
     1201 gcctcgccat accccgtaac gactgctctc agcacccctg aatccataac ggcattgtca
     1261 tttttcctga gctgctccgc gcgactacgt tcagtatctt cctgttcctt ccggcgcaat
     1321 tcctcttcac gtttaagcaa ctgggctctg agagctgcaa ggccggattc cccccactgc
     1381 tgccactgtt tttcaaatgc catctgactc gcgggaatgt cctgtttctc caccttccgg
     1441 taatgctgtc gtaatttttc catttgctgg ctcagagact ggatttttcc ctgctcacta
     1501 cggtattccg gtgccgccca ttgcatcagg ggaatacggg aaaaaccgga cgaaaacggc
     1561 gatgattttt tcaggctggc agcatggctc atcgcttcct ttcgttcggt gttcagctct
     1621 tcccagcgtc tgcgcgtcgc ctgcatatct gccatcatcg acatggcatt actggtgagt
     1681 ttccatgccg agttactgtc cttcggcaac gcttcgataa tgtcacgtag tgtgtacgaa
     1741 gttgcgttgt caggaagccc gatattcaga aggtcagcca cacggcgggc aggctctgtc
     1801 agccctccgt tcctgagttc ggtgcgggaa atctgtattt ccaggttaag cctgttaatc
     1861 agacgattct gttcgccgcg ttctgtttcg ataccgcgtg tttccatcgc acaggcagac
     1921 tttccgaggt gaataccagg cgttttctca agcccctgtc gctcgtatga acgatggtct
     1981 atctcttcct gataaccggc gttcgccagt gccctgtttg catggtcagc ccatgatgcc
     2041 cgccacgtct cggcatgtgt ccggtcgttc cagtccctga cctttccgct gaacccagca
     2101 ggtccgatag ccctcgtggt cagcatgatg tgcgcatggg gatttgtttt atccatgtga
     2161 tggaacgcca catcagcaat catgccctga ctgacaaagt tttctctgac aaaggcgaga
     2221 acggtttccc gtgcggcatc ctgcggcagt tcgtggggga tggcgagttc aatttcccgc
     2281 gcaagttgtg agttcttccg gcgttcggcc ttctcaacgg cattccagag ttcggcacgg
     2341 ttcgtacacc aggcaggggc ggtggcgggg gtcagaatca cagcctcagc cacaccgctt
     2401 ttgcgggtgt agtcgtgagt cagtccggta cgctcatcaa gcatcagtga accggaacga
     2461 taggcagcgg aagctacggc gctgtagccg ctgcttctgg agataatttt catgctgaga
     2521 tgataaatcg ccatttctgc cgtaatgcct ttttgtagtt ttctttcagc cccgcagggc
     2581 gcacacaatc gaaacggagt ggagattgta taagtgcgca cttcgtgatt tgccgtgcat
     2641 tatgccagct cagtttttaa actaccagtg acgcgctaaa atgcgatttt tcatcgcgca
     2701 cggagttaca tcatgagcag aacgctcgaa cagaagattg ctgatgctga ggcgagatta
     2761 cagcgcctga aggcgaagag ccggagcctg gacacagcgc agaaggttgt tgtgggtgct
     2821 gcattgctgg caaaggtcag aaagccggag gaggtgcagt tgcgtgcctg gttactccag
     2881 ttcctgaagg cagaggtgac acgacaggct gatgtgacgc gcatactgcc gctgattaac
     2941 gagctggagg cgctgccaga acagtgattg tatcgtccgc tcgccgcagc cccagcgacc
     3001 gagcgttagc gagcgagtgg gcgaggaagc ggaagtgaat cagtcgccag gttgagcaca
     3061 tacgcacggt ttttcggcag gtgatatgcg ctgaacccgc atgaatactg ggtttccaca
     3121 gggttggagg gggtgtattc gaaacttgca attctgcttt attgcaacgc attgttttta
     3181 ttggtttttt gttttcagca ccccaagtta tccacaggct cgccgttaag tttttaagat
     3241 cttattttta gatctttttt tttaaaacct ttttaggtct tgtaacacca tgattttaaa
     3301 gatgttctgg caagataagt ggagcttttg ggggtgaaag gtggagcttt tgggggtgaa
     3361 aggtggagct tttgggggtg aaaggtggag cttttggggg tgtaatccac agaaaaaggg
     3421 ggttgcaaaa aaaaaacttc cacccatatg ataagtggcg tacagtaaaa aaacgccacc
     3481 aatagcaacg agcaagcgaa atgtcagatc tgatagctta caaatcaaac gcccttatcg
     3541 aggcaagcta caagctcact ttgcaggaac agcgcttcct gctggtctgt atcagccgcc
     3601 taaaatccgg agaagattcc ccttcgccgg atgaacaaaa aaccatgacc atcacggctg
     3661 cagagtttca tgatgccttt cctgacatgg gacggcagca cgctgaagct aggctcaagg
     3721 aagcgataga tcgcctctgg gacaggtcag taatcatcaa gaacgaggaa aaacgagaag
     3781 agttccgctg ggtgcagtac cgggcgcagt atttcaaagg tgaggcgaaa gtagatatca
     3841 ccttctccga tgccatcatg ccgtatctga cacagctcaa ggggcagttt acgcgggtag
     3901 tcgtgaagaa cgtctccagc ctttccagca cctacagcat ccgcatttac gagctgctcc
     3961 agcagttccg cagcaccggt gacagaacga tagctatcga tgatttccga accatgcttg
     4021 ccgttgaaga taaatatcaa caattcaaag acttgaataa gat
//
