LOCUS       NZ_CP019023             3256 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_881 plasmid pEC881_6, complete
            sequence.
ACCESSION   NZ_CP019023
VERSION     NZ_CP019023.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511150
            Assembly: GCF_002012225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Stoesser et al,N.
  TITLE     Unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002012225.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:39:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,524
            CDSs (total)                      :: 5,400
            Genes (coding)                    :: 5,174
            CDSs (with protein)               :: 5,174
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 83 of 226
            Pseudo Genes (incomplete)         :: 157 of 226
            Pseudo Genes (internal stop)      :: 44 of 226
            Pseudo Genes (multiple problems)  :: 51 of 226
            Pseudo Genes (short protein)      :: 1 of 226
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019023.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_881"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC881_6"
                     /geo_loc_name="Colombia: Cali"
                     /lat_lon="3.45 N 76.54 W"
                     /collection_date="2013"
                     /collected_by="Merck Study for Monitoring of Antimicrobial
                     Resistance Trends (SMART)"
     gene            461..573
                     /locus_tag="BE930_RS30355"
                     /pseudo=""
     CDS             461..573
                     /locus_tag="BE930_RS30355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(804..1100)
                     /locus_tag="BE930_RS00045"
                     /old_locus_tag="BE930_00045"
     CDS             complement(804..1100)
                     /locus_tag="BE930_RS00045"
                     /old_locus_tag="BE930_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609270.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_000609270.1"
                     /translation="MINLVDPVHPGVFIREMFMEPFEISAADLSEKLHVSPSTLSRVLN
                     GKADLSVEMALRLSRVLGRSAESWINMQTQYSLNASRKSPNVIKELDELVPIF"
                     /name="HigA family addiction module antitoxin"
     gene            1445..1726
                     /locus_tag="BE930_RS00050"
                     /old_locus_tag="BE930_00050"
     CDS             1445..1726
                     /locus_tag="BE930_RS00050"
                     /old_locus_tag="BE930_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000586174.1"
                     /translation="MIINIKHKGLRKFAETGNNAGIPQQFTQRLKLILTALSTATNLQD
                     MMDLKALRCHPLKGDRQGEYSLEVNGNWRVVFKLEEPNVFDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2870..3064
                     /locus_tag="BE930_RS00060"
                     /old_locus_tag="BE930_00060"
     CDS             2870..3064
                     /locus_tag="BE930_RS00060"
                     /old_locus_tag="BE930_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041633.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2356..2611)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="89.0"
                     /evalue="1.21e-116"
     Typing          complement(2736..2834)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          complement(3137..3219)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.79e-36"
     Typing          2..530
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.727"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 gcacagatgc gtaaggagaa aatgcgcgtc caggcgcttt tccgcttcct cgctcactga
       61 ctcgctacgc tcggtcgttc ggctgcggcg agcggtactg acttacacaa aaacggcagc
      121 acggttatct acagaatcag gggataaggc cggaaagaac atgtgagcaa aagaccaaga
      181 acaggaagaa ggccgcgccg caggcgtttt tccataggct ccgcccccct gacgagcatc
      241 acagaaatcg acgctcaagt cagaggtggc gaaacccgac aggacttaaa gataccagac
      301 gtttccccct ggtggctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      361 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      421 tctcggttca gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      481 gcccgaccgc tgcgccttat ccggtaacta tcatcttgag tccaacccgg aaagacatgc
      541 aaaacaccat tggcagcagc cactggtaac tgatttagag gagttagtct tgaagttatg
      601 cgccagttaa ggctaaactg aaagaacaag ttttggtgac tgcgctcctc caagccagtt
      661 accctggttc agaaacttgc cggttactga accttcgaaa aaaccacctt gccgggtggt
      721 tttttcgttt acgagaaacg atacgatcca gttgcctgag agtcaagaca gaaaaaggcc
      781 gcatatagcg gccttttctt ttctcaaaat ataggaacca attcatctaa ttccttaatg
      841 acattaggtg acttcctgct cgcgttaaga ctgtattgag tctgcatgtt tatccacgac
      901 tcggcactgc gaccaagaac tcgagaaagc cttaatgcca tctcaacact taaatcagct
      961 tttccattca gcactctact tagagtggaa ggtgatacat gcagtttttc agacaggtcc
     1021 gcggctgata tttcaaacgg ttccataaac atctcacgga taaaaacccc tggatgtact
     1081 ggatcgacaa gattaatcat accttcctct gaagcctata ttagttttaa aaagataggc
     1141 ctattgcgtt ttcagattgc gcattgcgca taacgcaact cattgttgac taaaaaaaag
     1201 aatgacatta tgatgcacat tggtgcgtct aatcattcct tatgatgtgg gtacttgatg
     1261 tgaaattcaa ggccccggca ttgtacagcg catcaaacca atgtcaacag attaaaatgc
     1321 gacttggtta agtaaaacct acgtcattta ttcaaataga gcaaggggtt tgcaatggaa
     1381 atacggatga accggaaaaa taaactgcga tgtttctatc taaacaaata tgggatggtg
     1441 attaatgatc ataaatatca agcataaagg tttgcgtaag tttgccgaaa caggtaacaa
     1501 cgcaggaatt ccgcaacagt ttactcaacg tctgaagctg atcctaacag ccttaagcac
     1561 agcgacgaac ttgcaggata tgatggattt aaaggcattg cgctgccatc cattgaaggg
     1621 agaccgtcaa ggagagtatt cgttagaggt caatggaaac tggcgcgtag tgtttaagct
     1681 cgaagaacct aacgtctttg acgtggatct cgaggattat cactaagaag gtccttaaag
     1741 catcaaagtg acagtatcga tgctttaggg tctcataaaa aagcaaaacc tcggggtaag
     1801 ttatatgaag tattttctgt accagatagt ccctatagca gccatcatta gcgcagcaat
     1861 tctagcgcta aaaggtatca atggctgggg gtggttctta actgttgggt tcctgcttgc
     1921 aggggcagat acagtaaagg ctcagttatc taagtagtaa taaacactat caatagtggt
     1981 tggataattg ccaaaaaagc ctctatctgt tctgaaacag acaacaagga aaaatcggtg
     2041 taagccacct ccataggagg tggtattgat atcaacatag ataaaaccat cccgtaaatc
     2101 acagcattta ccataacgtc gcattacgcg caccgctaaa acgcgctgaa cgcgattctg
     2161 ccgcgaacac cgcaaaaaga gatgttttac caagcgaaaa ccggaaaaat gcgtcaggat
     2221 cgctttcagc tcactgcata gctatgcatg aaagtgaatg gcgatcggtt tgggcgcata
     2281 cggtgcccat gagacacatt ttcggacatt ttcagttcgt gaatccctga ctcattgcta
     2341 catatctgaa cagacacccc tagcagcgcc cccaggggcg tcctgtaaaa aaacacaccg
     2401 cgccgctagc agcaccccta atataaaatg atgtttttta tgaaaatagt cagtaccacc
     2461 cctaaaaagc ggtgttggcg cagctacaac accgcgccga caccgctttt ttaaatatca
     2521 caaagagagc aagaagaact aatttgtcat aactctctat ttataaagaa aaatcagcaa
     2581 aaaattgttt ttgcgtgggg tgtgggcatt tcaggcatga gaacagactc gcgtttttcc
     2641 ggagtatcag cggaattatt gattaagaaa cactcgacgc agagcgaaat tttatgcaac
     2701 agcggctgca ctgcacaggg attatttatc agttgatatg ctgtgccatg atacgtccta
     2761 aaaaaacagg acaggatgtg caattgtaat accgtcgccc gcaacgtctt acaattacca
     2821 cctggtcagg gtccacccga cacccggtac tgacggagaa caacggaagg tgaacaaaca
     2881 gcaacaaact gcgctgaata tggcgcgatt tatcaaaagc cagagcctga cactgcttga
     2941 aaaactggat gctctggatg ccgacgaaca ggcggccatg tgcgaacgac tgcacgaact
     3001 cgcggaagaa ctccagaaca gcatccaggt tcgctttgag accgaaagtg aaacaggaac
     3061 ataacgaagc tgacgcgccc tggcgggctt gtctgctgcc gaaaacgctc gcggacaagc
     3121 ccgccagggc gcgtcagcgg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
     3181 ggagtgtata ctggcttaac tatgcggcat cagtgcagat tgtatgaaaa gtgccccatg
     3241 ccgggtgtga aattcc
//
