LOCUS       NZ_CP019022             3087 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_881 plasmid pEC881_7, complete
            sequence.
ACCESSION   NZ_CP019022
VERSION     NZ_CP019022.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511150
            Assembly: GCF_002012225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002012225.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:39:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,524
            CDSs (total)                      :: 5,400
            Genes (coding)                    :: 5,174
            CDSs (with protein)               :: 5,174
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 83 of 226
            Pseudo Genes (incomplete)         :: 157 of 226
            Pseudo Genes (internal stop)      :: 44 of 226
            Pseudo Genes (multiple problems)  :: 51 of 226
            Pseudo Genes (short protein)      :: 1 of 226
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019022.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_881"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC881_7"
                     /geo_loc_name="Colombia: Cali"
                     /lat_lon="3.45 N 76.54 W"
                     /collection_date="2013"
                     /collected_by="Merck Study for Monitoring of Antimicrobial
                     Resistance Trends (SMART)"
     gene            complement(1409..1816)
                     /locus_tag="BE930_RS00020"
                     /old_locus_tag="BE930_00020"
     CDS             complement(1409..1816)
                     /locus_tag="BE930_RS00020"
                     /old_locus_tag="BE930_00020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053237188.1"
                     /translation="MGFIGNLFSMFTGKPTAYDKLTLIEFGDWLETYSSSNLLSQSSMA
                     TALMVQGVNLLVQNMGNEYSVLYSFIRNKKVSSKKVFDDLFIPFIVHCKQNDNAGEFDY
                     RTLMGMPARATMAYMIATIYDSDPQLLFDIE"
                     /name="hypothetical protein"
     gene            complement(1820..2734)
                     /locus_tag="BE930_RS00025"
                     /old_locus_tag="BE930_00025"
     CDS             complement(1820..2734)
                     /locus_tag="BE930_RS00025"
                     /old_locus_tag="BE930_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_053237187.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPDNGHAHLLYALKTSIRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHGHWKIAVWQPELYTLDWLADFLDLNAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPAYDQWLQACYDRASAYNQQFAAPLD
                     DNEVIGIAKSIAKWTFNIFSKEKFEAYVRDSHSSKIQSIRGRKGGLISKRGVSPRSQRT
                     SQPWLDLKISRATFYRRKKASEA"
                     /name="replication initiation protein"
     Typing          complement(952..1161)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.252"
                     /coverage="97.0"
                     /evalue="7.33e-68"
ORIGIN
        1 tcagccgggg taagtcctgt ttccgatgtt cttcttaccc gaaaacggtg aacggggacg
       61 caatttattg cgtggccgtg aggcgttgcc ggcacagccg gaaaaaccat tttcagcacg
      121 tgcaggaggc ccaggaagag ggggcggatg tacgaatagt tcagaggtca gatctgacat
      181 aaaaatgatg taaaagtgac gtaaaagtga tgtacttttg atgtaattct gttgtacacc
      241 cacgatctga cgtaaaagtg atgtaattgt gatgtacttt tgatgtaatt ctgttgtaca
      301 cccgcgttct ggggtaaagc gatgcaattt tgtagtttat gtgtagtcgc ttatcaggcg
      361 ggttctgctt ttgtagtctt ctcgtagttt atatgtagtc gcttatcagg cgagctctgc
      421 ttttgtagtc ttcttgtagt ttatgtgtag tcgcttatca ggtgggttct gcttttgtag
      481 tcttcttgta gtttatgtgt agtcgctgaa cccatagggt catacgagca tgaccctttt
      541 ccggaaaagg acgcttttta gccagaatca tccttaacct gcggcgccag cactcgggag
      601 tggtcgtttt atatacaaaa aaatgacata cacctaacgc agcggcgatt ttcatcgcaa
      661 ctgcatgaat tacataaaaa aaatgggatg caccgtttat tacggtaata cgcaggtatt
      721 aaaatcatct gtaatcgcat gaaaataaag aaataagatg taataccggc gtattaccac
      781 gtacgcgggc aagcgaaaaa agctataacg cttcccggcc gacaggtggc ccagaatcca
      841 caggggcatg caggttctct tcagtaacct tcgccattaa atcaaaaagg tcatattcgc
      901 cgggaagctg cgtatcagtc gaatcccgtt gttcatcatg ttcagacaga atggggtgat
      961 atccagtagc ggagaggttg ttagcgggtt tcggggcgca gccctgaacc agtcacgtag
     1021 cgctagcgga gtgtatactg gctaaatatg ttacggcgga gtaacactta atgaaagtgc
     1081 ttcatgtgac aggggaaaat gcagctacag ggcgtacgga agaaaatggg ggttacggtg
     1141 atatgacgcc tcctcgctca ctcggtcgct gccgctcctg ccgttcggct gcggcgggcg
     1201 ttacctgcac acggataaga gcaaaggcat acggcatgac aagcggggca gaaaggagct
     1261 gcgtatcagc agcatcccgt tgttcatcat gttcagacag aatggggtga tatccagtag
     1321 cggagaggtt gttagcgggt ttcggggcgc agccccctga gaaggggctg ttatctgata
     1381 taggcttatt tggtctcaat cggtgctgtc actcaatatc aaataaaagc tgggggtctg
     1441 agtcgtaaat agtagctatc atataagcca tcgtggctct ggcaggcatc cccatcaaag
     1501 tacgataatc aaactctccg gcattgtcgt tttgcttaca atggacaatg aacgggataa
     1561 ataaatcatc aaatactttc ttggaagaaa ctttcttatt cctaataaaa gaatacagta
     1621 ccgagtactc attccccata ttttgaacaa gcaaattaac accttgaacc ataagagcgg
     1681 ttgccattga tgattgggat agtaagttag atgaactata tgtctctaac cagtcaccaa
     1741 attcaatcaa agtaagcttg tcataagctg tcggtttacc agtaaacata ctgaatagat
     1801 tacctataaa tcccataagt catgcctccg aggctttttt tcttctgtaa aatgttgcgc
     1861 gagatatttt taagtccagc catggctgac ttgttctttg ggatcgaggt gaaacacctc
     1921 tcttcgagat taagccgcct tttcgccccc tgatagactg gattttcgat gagtgagagt
     1981 ctcttacata tgcttcgaat ttttccttcg aaaagatatt gaacgtccat ttagcaatgc
     2041 ttttagctat ccctattacc tcattgtcat ccagaggagc tgcaaattgc tgattatagg
     2101 cgctggcacg atcatagcag gcttgcagcc actggtcgta cgcaggccat ccctgacgaa
     2161 tggcgcggta tgcccacttg cgggttttgt cgaacagggt gcagttacgg cctaagccgt
     2221 agtcggcgac gatttcttta tcgttcgccg cgttcaggtc gaggaagtcg gccagccagt
     2281 ccagcgtgta gagttccggc tgccagacgg caattttcca gtggccgtgg ttcggatttt
     2341 tgcagattag gcccgaatac cccgcgtcag cctctaattt tttacgtagc gcgttctcca
     2401 cggcagcggc gtagcgcagc ggcttcattt ttccgtccgg tgcggtgcga atcgaggttt
     2461 tgagggcgta cagcagatgc gcgtgtccgt tgtcggggtt ggtgatggtc agcgtaggag
     2521 ctggcgcgtt cctgtcgctc cagtcaatgg ctgcgccggc gcgatcaacg tcaaatccca
     2581 gccagaacat ggcgtgaggc tggttgaact ggatatattt cgccaggatt gcacgttctt
     2641 ttcccgcaat gcgcacacca aagtgcagat catccgagaa gtacggctta tgcggtaagc
     2701 gctcgttaaa aagttcgagt gcggcgttat gcaatgtgcc ctgcgcttat ggcgggctgt
     2761 tgtctggcac ggttcatgtg ctaatatcct ttcaaggttt cgacctggcc ctggttatca
     2821 tgcgatccgc caagatatga catgatttcc ggggcttagt tttttctggg cttaaagtta
     2881 caccgtcttg cgcgatctgt cgatctcacc acgatttacc ataacatccg ttgcgtgcac
     2941 cgcctcagag gctctcaggg cgtttctgac gggaaaccgg taaaacaggc aacaaaccac
     3001 ccgaaaaccc gccagaaacg cgcaggctct gtttttacgg catgcatgag tatgcgcgca
     3061 agtgcatagc gcggaaattc acgccat
//
