LOCUS       NZ_CP019003            12602 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_AZ155 plasmid pECAZ155_3, complete
            sequence.
ACCESSION   NZ_CP019003
VERSION     NZ_CP019003.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511168
            Assembly: GCF_002012125.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12602)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002012125.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:27:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,043
            CDSs (total)                      :: 4,919
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 233
            CDSs (without protein)            :: 233
            Pseudo Genes (ambiguous residues) :: 0 of 233
            Pseudo Genes (frameshifted)       :: 77 of 233
            Pseudo Genes (incomplete)         :: 149 of 233
            Pseudo Genes (internal stop)      :: 56 of 233
            Pseudo Genes (multiple problems)  :: 45 of 233
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_AZ155"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pECAZ155_3"
                     /geo_loc_name="China: Beijing"
                     /lat_lon="39.90 N 116.41 E"
                     /collection_date="2013"
                     /collected_by="AstraZeneca global surveillance study of
                     antimicrobial resistance"
     gene            complement(1..984)
                     /locus_tag="BE948_RS02450"
                     /old_locus_tag="BE948_02515"
     CDS             complement(1..984)
                     /locus_tag="BE948_RS02450"
                     /old_locus_tag="BE948_02515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_151166972.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENKGLSVARNVAIESSTGEYIWFLDSDDKISHGSLKLIDKI
                     TNEKNYGIIFFNTHVFFDTPELKKKGWHDYKRSTFGEMDALTFFEKEMTNKKLIVSACC
                     YVFRRELLNGNAFIPHIYHEDNHFIATLIFDNYSTICLSLDEALYLRRVRNDSIMTSKK
                     SMRHIIGYSTCIDELTKYKKTKGIQNKSFDRFVNGMVLSLFSIAETIKTKEAMNYVARY
                     KKHPDLSFKDRALINYPLIKKTYLKLKNGKVFNYLRNGIINKKSNP"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(1069..1437)
                     /locus_tag="BE948_RS02455"
                     /old_locus_tag="BE948_02520"
     CDS             complement(1069..1437)
                     /locus_tag="BE948_RS02455"
                     /old_locus_tag="BE948_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020936656.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            complement(1468..2696)
                     /locus_tag="BE948_RS02460"
                     /old_locus_tag="BE948_02525"
     CDS             complement(join(1468..2381,2381..2696))
                     /locus_tag="BE948_RS02460"
                     /old_locus_tag="BE948_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="WP_088895425.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQ
                     LFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYG
                     RGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLR
                     IHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKR
                     AHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQ
                     DVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACN
                     GLSDNRCLEI"
                     /name="IS3-like element IS2 family transposase"
     gene            complement(3156..3305)
                     /locus_tag="BE948_RS27765"
     CDS             complement(3156..3305)
                     /locus_tag="BE948_RS27765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_167776965.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHALVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            4390..4707
                     /locus_tag="BE948_RS02490"
                     /old_locus_tag="BE948_02555"
     CDS             4390..4707
                     /locus_tag="BE948_RS02490"
                     /old_locus_tag="BE948_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="WP_032242326.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAVNVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="lipoprotein"
     gene            5891..6025
                     /locus_tag="BE948_RS02500"
                     /old_locus_tag="BE948_02565"
     CDS             5891..6025
                     /locus_tag="BE948_RS02500"
                     /old_locus_tag="BE948_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072003336.1"
                     /translation="MHEPPVQSDRCVLSGNYRLESNPERHAKTPLAAAIGKQDVREMQ"
                     /name="replication initiation protein"
     gene            complement(6455..6823)
                     /locus_tag="BE948_RS02510"
                     /old_locus_tag="BE948_02575"
     CDS             complement(6455..6823)
                     /locus_tag="BE948_RS02510"
                     /old_locus_tag="BE948_02575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032242306.1"
                     /translation="MFSYLKAMYHQSKIQAELKAQIHEQTTVNAICHHPESIEIIAVCS
                     TDAYYRKRKDAAFLTTCSVLMRTLKDESVPMVLRKTAWRLLNERYQRIKLNQAYRIENF
                     LLVADFEYALEEHDELAE"
                     /name="hypothetical protein"
     gene            7168..7404
                     /locus_tag="BE948_RS02520"
                     /old_locus_tag="BE948_02585"
     CDS             7168..7404
                     /locus_tag="BE948_RS02520"
                     /old_locus_tag="BE948_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_032242304.1"
                     /translation="MTEMERQLLSALESLQTSYEQQKQAWQDSYSSLQRMFETTSQALE
                     RNDRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            7549..7881
                     /locus_tag="BE948_RS02525"
                     /old_locus_tag="BE948_02590"
     CDS             7549..7881
                     /locus_tag="BE948_RS02525"
                     /old_locus_tag="BE948_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032242302.1"
                     /translation="MSHAIEFIETTMFTRQIKQIATDDEIKELQKELIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSITDAEKAELKKLTQ
                     RLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7883..8167
                     /gene="nadS"
                     /locus_tag="BE948_RS02530"
                     /old_locus_tag="BE948_02595"
     CDS             7883..8167
                     /gene="nadS"
                     /locus_tag="BE948_RS02530"
                     /old_locus_tag="BE948_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_032242301.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFDELAAH"
                     /name="nadS"
     gene            8366..9242
                     /locus_tag="BE948_RS02535"
                     /old_locus_tag="BE948_02600"
                     /pseudo=""
     CDS             8366..9242
                     /locus_tag="BE948_RS02535"
                     /old_locus_tag="BE948_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946055.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            9387..>9651
                     /locus_tag="BE948_RS02540"
                     /old_locus_tag="BE948_02605"
                     /pseudo=""
     CDS             9387..>9651
                     /locus_tag="BE948_RS02540"
                     /old_locus_tag="BE948_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /name="IS1-like element transposase"
     gene            complement(9744..11288)
                     /locus_tag="BE948_RS02545"
                     /old_locus_tag="BE948_02610"
     CDS             complement(9744..11288)
                     /locus_tag="BE948_RS02545"
                     /old_locus_tag="BE948_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_201360865.1"
                     /translation="MKNYDVFYGGIFKTITDYKLLKIQKDIKNNMTALVSSFKWVGIGQ
                     LVKIATQLVSLFYLTHLIPPSSYGLLAMSSVVMNLASIFNDMGTGAAVIQRQEASIGFY
                     NYIYRINIFTGLFVMLLVIITSPLIVVYFNTPELYPVLFLLAFSFPISSLTITHKAKLE
                     KEMNFKVIIAIEIFSSLTGMVLAIVLANLGFGVYSLIFQTLTTISISTICFLKVSGLSL
                     SLRIKNISHSDKSGVIGFSGYLLSFNLINYFSRNLDSILIGRFFSSAILGVYSIAYRIM
                     LFPLQSLTLVFSRVFLPHLSKNINNTNNTNKQDYLNALKLILSLSAPLMLGLSATSFSF
                     TRIFFDPKWHLLGDLLHWLAPTAIIQSALSTTGSVFMAYGKTRWLFFLGCIGAFLMGLS
                     FIIGVHFDIKTLVYLYFFANLINSLPAFILLKNILKFSYIELVNVFFRTISPAFLMFLL
                     IKVWQHFSFFKNASIDFSFNVALGIFSYIVFYTLFNFNEVVRTGVIHKLRCMTKLK"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            11447..12144
                     /locus_tag="BE948_RS02550"
                     /old_locus_tag="BE948_02620"
     CDS             join(11447..11696,11696..12144)
                     /locus_tag="BE948_RS02550"
                     /old_locus_tag="BE948_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223368647.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     Typing          complement(3341..3514)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="5.58e-78"
     Typing          3481..3989
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5634..5999
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.185"
                     /coverage="89.0"
                     /evalue="2.91e-169"
ORIGIN
        1 ttaagggttg ctttttttat taataatgcc attccttagg taattaaaca ccttcccgtt
       61 ttttagtttt aaataagtct ttttaattaa tgggtaatta attaatgctc tatctttaaa
      121 tgataggtcg gggtgttttt tatatctcgc cacatagttc attgcttcct ttgtttttat
      181 tgtttccgct atagagaata acgacaaaac cattccattc acaaatctat caaagctctt
      241 gttttgtatg ccttttgttt ttttatactt cgtaagctca tcgatgcatg ttgaatatcc
      301 aatgatgtgc ctcattgatt ttttagacgt cattatggaa tcatttctta ccctcctaag
      361 atataacgcc tcatccaacg agaggcatat agttgaataa ttgtcaaaaa tcagggttgc
      421 tataaagtga ttatcctcat gatatatatg cggaatgaat gcattgccat ttaataattc
      481 ccttctaaaa acatagcagc atgctgacac tattagtttt ttgttcgtca tttctttttc
      541 aaagaaagtt aatgcatcca tttcaccaaa tgttgaacgc ttataatcat gccagccttt
      601 ttttttcagt tctggagtgt caaaaaaaac atgtgtatta aagaaaataa tcccataatt
      661 tttttcattg gttatcttgt ctattaactt tagacttcca tgtgatattt tatcatcact
      721 atctaaaaac catatatatt caccagttga tgattcaatg gcgacatttc ttgccaccga
      781 caggccttta ttttcctgat ttattacttt gattctttgg tcatcaaatg acagtatgat
      841 atctagactg ccatctgttg aaccatcatt tacacagatt atctcatagt cgttaaaatc
      901 ctgatctaat actgattgaa tacattccct taagtatttt tcaacgttat atacaggtac
      961 tattatcgaa aatttttttt tcacagtaat ctccaaacaa tatttagtta tactaacatc
     1021 aagttgttat ttatggaatg cgatttatca acggctttgt tgaataaatc agaatccttt
     1081 cttcctgcta aggctatctc tgaatgactc aaccactctt tgagattctt cttctgctcg
     1141 catgaagtca ttcccggaca gaaaaaaacc attgtcaggc ccactggctt cagagaaatt
     1201 cgtttctgca gcagacacag aatccacatc agcaagagac ggttcatctg tactttttct
     1261 caatctgtaa agagccttat caaacgcaac cagtgagata ttcaggccgt aacgttcatt
     1321 cagcacagtc acaatctctt ccctacgaat accatttttc ttccaggttt ctatatcgtt
     1381 cttggaatcc ctgacagctg caatggctgt tcttggacca gggttaaaat tcgccatata
     1441 ccgcccataa tgtctttgga tttgccccta tatttccaga catctgttat cacttaaccc
     1501 attacaagcc cgctgccgca gatattcccg tggcgagcga taacccagcg cactatgcgg
     1561 atgccattcg ttataatgct cgaacgcctc tgcaaggttc tttgctgccg ttaacccgtc
     1621 tggtttgggc atgatactga tgtagtcacg ctttatcgtt ttcacgaagc tctctgctat
     1681 tccgttactc tccggactcc gcaccgccgt gttcttcggt tcaagtccca acatccgggc
     1741 gaactggcgt gtttcattag cccggtagca tgaaccatta tccgtcagcc actccactgg
     1801 agacgacgga agatcgttgc cgaagcggcg ttccaccgct cccagcatga cgtcctgtac
     1861 tgtttcactg ttgaagccgc cggtagtgac cgcccagtgc agtgcctcac gatcacagca
     1921 gtccagcgcg aacgtgacac gcagtctctc tccgttatca cagcagaact cgaacccgtc
     1981 agagcaccat cgctgattgc tttctttcac ggccactctg cctgtatgtg cccgtttcga
     2041 tggcggtaca gcaggttttc gctcaagcaa cagcgcattc tggcgcatga tccggtaaac
     2101 acgtttggca ttgatcgcag gcataccatc aagttctgcc tgtctgcgaa gcagcgccca
     2161 tacccgacga taaccatacg tgggcagctc tccgataaca tggtgtatac ggagaagcac
     2221 atccgtatca tcagtgtgac gactgcggcg gccatccatc cagtcatcgg ttcgtctgag
     2281 aatgacgtgc aactgcgcac gcgacacccg gagacaacgg ctgactaagc ttactcccca
     2341 tccccgggca ataagggcgc gtgcgctatc cacttttttg cccgtccata ttcaacggct
     2401 tctttgagga gttcattttc catcgttttc ttgccgagca ggcgctggag ttctttaatc
     2461 tgcttcatgg cggcagcaag ttcagaggca ggaacaacct gttctccggc ggcgacagca
     2521 gtaagacttc cttcctggta ttgcttacgc cagagaaata actggctggc tgctacacca
     2581 tgttgccggg caacgaggga gaccgtcatc cccggttcaa agctctgctg aacaattgcg
     2641 atcttttcct gtgtggtacg ccgtctgcgt ttctccggcc ctaagacatc aatcatctgt
     2701 tctccaatga ctagtctaaa aactagtatt aagactatca cttatttaag tgatactggt
     2761 tgtctggaga ttcagggggc cagtctagtc ttacatttgt ctcataatag tccaaaataa
     2821 gagtaaaacg caaaaaatgt cttacaattg tcttattttt gtcctttccg gttcatggat
     2881 acatgaaccg cctgatgtgg aacgcttttt atcgatctca tgcgctgcca aagatagatt
     2941 cattctttaa aagtcaggtt acattatccc ttaaatggtc atacaccata cgcaaccgcg
     3001 attttcatcg caattgcatg aattaaaaga taaaaactga agtccccgtt tatttatgta
     3061 atacgcaacg tattacaacc acctgtaatt acatgaaaat aaagaaataa gatgtaatac
     3121 cggcgtatta ccacgtacgc gggtaagcga aaaagctata acgcttcccg gccgacaggc
     3181 ggcccaaaat ccaccagggc atgcaggttc tcttcagtaa cctgcgccat taaatcaaaa
     3241 aggtcatatt cgccgggaag ctgcgtatca gtcgaatcca gttgttcatc atgttcagat
     3301 ggcatggggt gatatccagt agctgagagg ttgttagagg gtttcggggc gcagccctga
     3361 accagtcacg tagcgctagc ggagtgtata ctggcttact atgttggcac tgatgaggga
     3421 gtcagtgaag tgcttcatgt ggcaggacaa aaaaggcggc accggtgcgt cagcagaata
     3481 tgtgatacag gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac
     3541 tgcggcgagc ggaaatggct tacgaacggg gcggagatgt cctggaagat gccagggaaa
     3601 tacttaacag ggaagcgata gggccgcggc aaagccgttt ttccataggc tccgcccccc
     3661 tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata
     3721 aagataccag gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg
     3781 gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca
     3841 gttccgggaa ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg
     3901 actgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga cacgacaaag
     3961 cgccactggc agcagccact ggtaacagga ttagcagagc gagttaatgt aggtggtgct
     4021 atagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     4081 tgcgctctgc tgaagccagt taccaggtta agcagttccc caactgactt aaccttcgaa
     4141 aaaccacctc cccaggtggt tttttcgttt tcagagcaag agattacgcg tagaaaaaaa
     4201 ggaactcaag aagatccttt ctatgctcaa ccaccgcatg gcccatagcc gcgctggccg
     4261 ttgccggtca gctccagaat cctcgaccaa tatggaacct tgcccctccc aaaattcaac
     4321 taaaaaaccc tcatttcctg ccgataaaat gggtatctct taactatgcc taaataaata
     4381 aggataattt tgaaaaaaat attcatccta tcggcagcgc tgatcatggc aggatgcgca
     4441 acaaagcaat atccacaggc cgtgaacgtc accggtccag aatcttccgc aatggattgc
     4501 aaagcggtag ctctggaaat cgccaaaacg cgcagtgtac aacaagaaat tgaggaaacc
     4561 ggcgaatttg acgggcgcac cgtattaggt ttcctgggtg attttgggat aggaaatggc
     4621 atggcaaaag gcgaagcccg caaaaaagca gctaaccggc taaatcagct ggaagccctg
     4681 aaggctgcaa agtgtgcaga tcaataaggt acaaaaaagc cctcggggct ttttttgtac
     4741 ctgccaatca gctgaattta ccataacatc cgttgcgtgc accatctcag acgccctcag
     4801 ggcatttctg cgccctcctg cctatttccg gcatctttcc ctgtccatat cgtgaaaaaa
     4861 cgcgcctgat gatttcttcg cgtctgcatg gctatgcaca cgaacgcatg gacgtcattt
     4921 tagcggctaa aatccggcga gcgccctgaa agccaccacg aaccccaccg ataacggtga
     4981 aagggtgcgc agtttactgt gcgaccgtga ggcgttggcc aggccgtcag gcatggcagg
     5041 atttattgct aagggggtcg gatttaacct ggattaatca gcacacagtg atgaacggga
     5101 ttcggaaggg gcacccttgc ggcgcggcga tgtatacatc gctaacgtgc cttggtcatt
     5161 tattgcaaga agggagaggt agcattttga tagcaaaaaa gtagcaaaag gatagcaacc
     5221 gaaagaccag aagactctgg agatagcaaa aaagtagcaa aatggtagca aaaagatagc
     5281 aaacatgcgg gagctaacca ggagtcttta gcgggccggc ggtactccaa aaacacattt
     5341 tgtcccgctg ttttgtgaat aacccagcat agctgtgggc acaaaacatt tcctgtccgc
     5401 tgctttaacc tttgtaatcc aggaacgtca ggtgacgtca ctttgcggcg ttagccctat
     5461 cctgcatcct atgaaaaaat gcagttacgg agtgtaaggt gccggacgtg aggccgaaga
     5521 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcggctgcg gcgagcggac
     5581 ggagaatatt cacaaaggca tatggcgatg aagacgagat aaagcgaggg aggaaaggcc
     5641 gcggcgctgc cggttttcca taggctccgc ccccctgaca agcatcacag aatctgacgc
     5701 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat cccaggcgtt tccccctggt
     5761 agctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta
     5821 tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
     5881 gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgtcttgt ccggtaacta
     5941 tcgtcttgag tccaatccgg aaagacacgc aaaaacgcca ctggcagcgg ccattggtaa
     6001 acaggatgtg cgagagatgc agtaagagtt cttgaagtga tggcctgagt gcggctacac
     6061 tgaaaggaca gatttggtga catgtctcgc acaagactgt taccgcggtt agaaaatctg
     6121 ccaggattag ctaaccttcg aaaaaccacc tgccagggtg gttttttcgt ttacagagca
     6181 agagattacg acgcaacaga aaggatctca agaagttcct tgctgtattg cgggtccgct
     6241 tggaaaaagg aaattatcct acgttaagcg ttttaagcta acgggtgatt tcgttccatt
     6301 gagcgcgaac ccctgaccgt aagggatgta cgaaatgatc cggctgtccg ttaatggttt
     6361 tgcttacttt cggacatgtt gtctacatgc tgtacgtaat cggacgttta agtcacatgc
     6421 acgttaactg tcggtattca tcgcctcact agtactactc tgctagttca tcatgttctt
     6481 cgagagcata ttcaaaatcg gcaacaagca agaaattttc tatcctataa gcctgattta
     6541 acttaattcg ttggtatctt tcgttcaata atcgccatgc tgttttccgt agtaccatcg
     6601 gtacggattc atcttttaaa gtacgcatga gcacagaaca ggttgtcaga aaagcagcgt
     6661 ccttacgttt tcggtaatac gcatcagtcg aacaaaccgc aatgatctcg attgattcag
     6721 ggtgatgaca gatagcatta accgtagttt gttcatgtat ctgtgccttg agttcagcct
     6781 gaatttttga ttgatgatac atagccttga gatagctaaa catgatttcc ccatcaaaca
     6841 gtaagtcgtg tcaattccta gagttaacac atttctgatc atgactcaca cgttacgttc
     6901 agtcggtagc catctgtgca aaaaatgcgc ttagcgtagg ataatttcct ttttccaagc
     6961 ggaccccaga gcggcgcagg ggtagcgatt tacaaggacg acaagcagaa gaacacctat
     7021 ctggtggtac tgccgaagaa agcgcatggc accgagacat acacatcgac agccggaaac
     7081 accgtgatga agtacaccgc gaaataaccc cgtcagacac cacagaaacg ccacagcacg
     7141 tttttatcac tgaggacagg aaaaaccatg accgagatgg aaaggcagct tctgagcgcc
     7201 ttagaaagct tacaaacaag ctacgaacag cagaagcagg cgtggcagga cagttacagc
     7261 agcttgcaac gcatgttcga gactacctca caggcactgg agcgcaacga cagggtatgt
     7321 ctgaacttga gcaatcaggt gaacgccttg cacgaacagg tcgaacgctt gagcagcgat
     7381 ctgagccgat taacacgctc gtaaagcagc aggaacggca gttacagcag gagaagcaag
     7441 caacccgcta tcgaggtcag tccatgtaac gtctgatggc acagaacgcg gcttttcaga
     7501 acacgcctct tggtgtacat tgtacatcaa aatatacagg agacagccgt gagtcacgcg
     7561 attgagttta tcgagacaac gatgtttacc cgtcagataa agcaaatcgc cacggatgat
     7621 gagatcaaag agcttcagaa agagcttatc gagtcaccgg acaaaggtga cctcatccag
     7681 aaaacgggcg gtctgcgaaa gatcaggatg gcgacgggga atcagggaaa gagcggcagt
     7741 gcgcgggtga tttacttcct tgccaccgca gaggtgattt atctggtgat ggcctaccct
     7801 aaaagcacca aagacagcat cacagacgca gagaaagcag aactgaagaa gctgacccaa
     7861 cgactgaaag acgaggtgta agatgagttt ttttgacgag ctgaaagcct ctctggaaga
     7921 ggccgttgaa atcaaaaacg gaaccaaagc ccccgcccgt gtgacccgct acgagctggc
     7981 agacgtcagg gctatcagag aaaacctgaa tattactcag agcgaaatgg cgaaagtgct
     8041 gggaaccagc ctcgatacca tcaaaagctg ggaaaccgga cgccgtaacc caaccggact
     8101 ggcagcaaaa gtgctggcca ccattcaggc taacccaaag ttttttgatg aactggcggc
     8161 gcattaagct gatgtgacag aagaaattcg acaggcgagg ccgttcgcgt gtaactttaa
     8221 cggcaacaga aaaaacaaag ccccgataat catgatttca acttggcgga agagacatga
     8281 taaatcaggg ctaggtataa aacctaagaa ggatattagc acatgaaccg tgccagacaa
     8341 cagcccgcca taagttcagg gcacattgcg taacgcagca ctcgaacttt tcaacgaccg
     8401 cttgccgcat aaaccgtact tctcggatga tttacacttt ggtgtccgca tcgcagggaa
     8461 agagcgcgct attctggcta aatacatcca gtttaatcag cctcacgcca tgttctggct
     8521 gggatttgac gttgaccgca tcggcgcagc cattgactgg agcgaccgca acgcccccgc
     8581 gccgacactg accatcacca accccgaaaa cggacacgcg cacctgctgt acgcactgaa
     8641 aacctcgata cgcaccgcgc cagacggaaa aatgaaaccg ttacgttacg ccgctgctgt
     8701 agagagcgcg ctacgtaaaa aattgggcgc tgacacgggg tattcggggc taatttgcaa
     8761 gaatccgaac cacagtcact ggaaaattgc ccgtatggca gcctgaactc tacacgctgg
     8821 actggttagc cgaatcactc gacctgaacg ctgcgaacga taaaaaaatc gtcgccgatt
     8881 atggcttagg tcgtaactgc atactgtttg ataaaaccag aaagtgggca tatcgcgcta
     8941 tccgtcaggg ctggcctgag tacgaacagt ggctacaagc ctgccatgaa cgcgcctgcg
     9001 cttacaatct gcaatttgca gtgcctttga atgagaatga agtaacggga atagcgaaaa
     9061 gtatctgcaa gtggacattt ttgaaattta ataagcaaaa tttttcactt atacaagcca
     9121 aaagaggaaa agttggtggt gaaaaatcat caggaggagg taggctgtct ctaggggagc
     9181 cttgggttgc tttaggtatt agtcgcgcta cattttttag aaaaaggaaa atttataatt
     9241 aagattcagt attttacata ctgaatctta attattgaat caccattact taagtgttga
     9301 tagtgttttt tttccagcag taacaactgt ttgtgatgat gccaacttgc tggtttagtg
     9361 tctaatggtg tttttgaggt gctcccgtgg cttccatttc catcagttat ccctcctgtt
     9421 cagctactga aggcgtggtg cgtaacggta aaagcactac cggacatcaa cgctatctct
     9481 gctctcactg tcgtaaaaca tagcaagcaa ctacagttca cttacaccgc ctctgagccc
     9541 ggtacacacc agaaaatcat tgatatggcc atgaatggcg ttggatgccg ggccacatcc
     9601 cgcattatgg gcactgtcct caacacgatt ttacgacact taaaaaaatt aggccgaagt
     9661 cggtatcggg cattatctga acataaaaca caagcagtaa gttggagtca ttaccaattc
     9721 atttaatgaa tttaataaat gaatcacttt aacttggtca tgcatcttaa cttatgaatt
     9781 actcctgtcc taacaacctc attgaaatta aacaaggtat aaaaaacaat ataactaaaa
     9841 atacccaatg cgacattaaa agaaaaatca atgctggcat ttttgaaaaa ggaaaaatgc
     9901 tgccatactt taataagtaa gaacatcaaa aaagctgggg agatagttct aaaaaacacg
     9961 tttaccaatt caatgtaact aaacttcaag atattcttaa gtaaaataaa tgccgggagt
    10021 gaattaatta agtttgcaaa aaaataaagg tagaccaatg tttttatatc gaaatgaaca
    10081 cctataatga aagacaaccc catcaaaaaa gcgccaatgc aacctaagaa aaacaaccac
    10141 cttgtttttc cgtaggccat aaaaacagaa cctgttgtac tcaaggctga ttggataatc
    10201 gctgtaggtg ccaaccaatg caataagtct ccaagtaggt gccacttagg atcaaagaaa
    10261 atcctcgtaa aagaaaaaga tgtagcagat aaacctagca tcagtggcgc gcttaatgat
    10321 agtataagtt ttaatgcatt taagtaatct tgtttattag tgttattagt gttatttatg
    10381 tttttgctaa ggtgtggcaa gaaaactcta gaaaaaacaa gcgtcaggct ctgcaacgga
    10441 aataacataa tgcggtaggc aatcgaatag accccaagta ttgcggatga gaaaaaacgg
    10501 cctattaaaa tagaatcaag attcctggaa aaataattta ttaaattaaa agacaataaa
    10561 taaccactaa accctataac tcctgacttg tcactatggc tgatattttt aattctcaat
    10621 gataaactca acccggaaac cttcaaaaaa catattgtgg atattgatat tgttgtcagt
    10681 gtttgaaaaa tcaaactata aacaccaaag cctaaattag ctaaaactat tgccagcacc
    10741 attcctgtaa ggctagagaa aatttcaatt gcaatgatga ctttaaagtt catttctttt
    10801 tctaattttg ctttgtgagt tattgttaag ctggaaattg gaaagctaaa agccaataga
    10861 aatagaaccg ggtatagctc gggtgtgtta aagtacacga caattaatgg ggatgttatt
    10921 attacaagca gcataacaaa caacccagta aaaatgttta tcctgtatat gtaattataa
    10981 aaaccgatcg acgcctcttg tcgctgaatt actgccgccc cagttcccat gtcattgaat
    11041 atgcttgcta aattcataac cacagatgac attgctaata gtccatagct cgatgggggg
    11101 attaagtgcg tcaagtagaa taacgaaacc aattgtgttg ctatttttac aagttgtcca
    11161 atgccaaccc atttaaaact actgactagt gcagtcatat tgtttttaat atccttttga
    11221 attttcaata atttataatc cgtgatggtt ttaaagatac caccataaaa gacatcataa
    11281 ttcttcaagc tatcatctac gttgtagatt ccgatttagc taaactttag gactttataa
    11341 gttcgtatcc cgtatgatta taaggcatta tgatcatctc caccaatagt gggtaatgct
    11401 gccaacttac tgatttagtg tatgatggtg attttaaggt gcttgcgtgg cttccatttc
    11461 catcagatgt ccttcctgct ccgctactga aggcgtggtg cgtaacggca aaagcactgc
    11521 cggacatcag cgctatctct gctctcattg ccgtaaaaca tggcaactac agttcactta
    11581 caccgcctct cagcccggta cgcaccagaa aatcattgat atggccatga atggcgtcgg
    11641 atgtcgcgcc agtgcacgca ttatgggcgt tggcctcaac acggttttac gtcacttaaa
    11701 aaactcaggc cgcagtcggt aacctcgcgc atacaaccgg gaagtgatgt gattgtctgc
    11761 gctgaaatgg acgaacattg gggctacgtc ggtgctaaat cacgtcagcg ctggctgttt
    11821 tacgcgtatg acaggatacg gaggacggtt gtggcgcacg tcttcggtga acgcactctg
    11881 gccacactgg agcgtcttct gagcctgctg tcggcctttg aggtcgtggt atggatgacg
    11941 gatggctggc cgctgtatga atcacgcctg aagggaaagc tgcacgttat cagcaagcga
    12001 tatacgcagc gcattgagcg acataatctg aatctgagac aacatctggc aaggctggga
    12061 cggaagtcac tgtcgttctc aaaatcggtg gagctgcatg acaaggtcat cgggcattat
    12121 ctgaacataa aacactatca gtaagttgga gtcattaccc ggtctgcgtt gtcgggaaga
    12181 tgcgtgatct ggggttagtt tggatataga gaattattgt acggtaagcc tgttttttga
    12241 acagacttac ctatagcaat agggtgtact cccgcgctat acggcatttt cagaggtttg
    12301 accttaatcg gataacctaa atgcgatact gcaagagcga caacttaaaa gcgatagcca
    12361 ctgacgcaaa atcaacccct tacgaaccaa gattggttaa ataatgcgct taacgtacaa
    12421 aaatttccga tctccaaact gaccccgatc tgatccttca actcagcaaa agttcgattt
    12481 attcaacaaa gccccggaaa gcgtgcgtat cgcctgaaaa cccaacccgt cacggggata
    12541 cctatccgaa atcttattta ttcaacaaag aaaaaccttt attaattatc gctttgcttt
    12601 ta
//
