LOCUS       NZ_CP018997             4921 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_AZ153 plasmid pECAZ153_3, complete
            sequence.
ACCESSION   NZ_CP018997
VERSION     NZ_CP018997.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511160
            Assembly: GCF_002012245.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002012245.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:51:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,216
            CDSs (total)                      :: 5,103
            Genes (coding)                    :: 4,891
            CDSs (with protein)               :: 4,891
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 73 of 212
            Pseudo Genes (incomplete)         :: 159 of 212
            Pseudo Genes (internal stop)      :: 39 of 212
            Pseudo Genes (multiple problems)  :: 50 of 212
            Pseudo Genes (short protein)      :: 1 of 212
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018997.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_AZ153"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pECAZ153_3"
                     /geo_loc_name="China: Beijing"
                     /lat_lon="39.90 N 116.41 E"
                     /collection_date="2013"
                     /collected_by="AstraZeneca global surveillance study of
                     antimicrobial resistance"
     gene            complement(378..1292)
                     /locus_tag="BE940_RS00030"
                     /old_locus_tag="BE940_00030"
     CDS             complement(378..1292)
                     /locus_tag="BE940_RS00030"
                     /old_locus_tag="BE940_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271348.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            3029..3238
                     /locus_tag="BE940_RS00035"
                     /old_locus_tag="BE940_00035"
     CDS             3029..3238
                     /locus_tag="BE940_RS00035"
                     /old_locus_tag="BE940_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(3304..3837)
                     /locus_tag="BE940_RS00040"
                     /old_locus_tag="BE940_00040"
     CDS             complement(3304..3837)
                     /locus_tag="BE940_RS00040"
                     /old_locus_tag="BE940_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(3840..4766)
                     /locus_tag="BE940_RS00045"
                     /old_locus_tag="BE940_00045"
     CDS             complement(3840..4766)
                     /locus_tag="BE940_RS00045"
                     /old_locus_tag="BE940_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     Typing          complement(518..1292)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(3844..4766)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaaacaac gacgggaacg caatcaccgg ctctttctcg cggctgggct actgatcgaa
       61 gcaggactgg tagacacaaa aacaggcgag ctatgctata aaaaagacag gatattacaa
      121 gcacttaagg aacttaagta cgatctggag acatccccaa acccagacgc gtaagtgcct
      181 ccatccgtgt tccgattccc tggtgcttcc tcgctcattc gctcgctacg ctcggtcatg
      241 agactgcggc gagcactacc accacaatca cgcggaaagc cgcgccattg cagggctgaa
      301 cggggaaaaa cggcaaaaaa cctgctgctc aaaaagacgc cagaaaggcg ctgttatctg
      361 atataggctt atttggtcta ctctacgaga cctcgttttt tcagttggta ataccaggcg
      421 cgactgatcc ctaatgcctc ccacggtttc agcgtgcgtg cagatgtagc tactgtgctg
      481 cgcttagact tagaaccgcc cttgcgaccg cgtgcagcct gaatttctgg cgtgtgcgta
      541 tcagccacat actgtgcgaa agtttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
      601 ctcttgccaa tagcccgaca ttcagccagt gaaagcggaa cgggaagcga tgcattgtac
      661 atttcgacac gctggatcac cgcttcaagc cactgtgaga acgctggcca gccctgacga
      721 atcgccctgt aagcccattt gcgcgtcatt tcgaacaggt ggcagtttcg ccccatcccg
      781 taatgtttat cgatgctacg acgctctgag gcgctcaaat cgagataatc agccagttca
      841 tcgagggtat aggcttcctc gcgccactcc atcaccagcc attccaggtg gaacggattt
      901 ttgcaaatca ggccgctgta gttcacatcc gcacaaagtt tctcacacag cgaacgttca
      961 accgctgcgg catatttcag cgccttaacc gaggcatcag gcgcggttct cacggcgatg
     1021 ttgagtgcgt agagcaggtg agcgtgtccg ttcacaggat ttttaacggt gatgttcggc
     1081 gcgggagcgt tccggtcgct ccagtcaatc gcagccccct cgcggtcaac gtcaaaaacc
     1141 agccagaact gcgcatgagg ctggttctgc tgtatgtacc gcgcaagaag cgcacgccct
     1201 ttgccggaga tgcgaagacc aaaagcgaga tcatccgtgt gatagggacg atggggtaaa
     1261 ttttcgtcga agtattgaag cgcggcgctc atgtgttacg attctctccg ggttatgcga
     1321 cctacacgcc ctctgtattt catgttgaat cttgccggat ctggacatga tttcaggggc
     1381 gtttgttttt cagtcgctta cactacaccc aacggcaaat cctgtcgaac cttcccgaaa
     1441 acccgatcca tttaccataa ctcccgttgt acgcccccct taaaacgccc tcagtcgctt
     1501 tctggcgcaa aaaacgcaaa aagacaagaa aacaccaggc caaaccagaa aaacgcgtca
     1561 gaatcgtttt cagggcgttg cataactatg cacgaaagcg atgtgcgatc ggtttcgtgc
     1621 gttctgaaca ccgaacggct caaaacgaca agttttctcc cctggtgcgt ggcgctctga
     1681 cggggtttcc tgtttccaaa cttcacatct cgccaggaca aaaaaaaacc gacctggggt
     1741 cggtggcaag aatcagagtc tatgggaaat attttagtta tcggatgctc aatattcaca
     1801 aacgcaaaaa atgcctcagt gatgccctaa aacaaccttt ccagaaggag tccgcgcttt
     1861 acgatgcacg gaagagataa aaaaccccac aaaagtgggg aaccaagaga aacaacaatt
     1921 acaggttgtt ggttgtgttc gaacaacgat gaagtagcta cgctaaataa taattaccgt
     1981 acaggcccta catattcaac actaaattta agatcctgac gaaggattta taaatgcacg
     2041 aaaacacata gacagacaaa ttcactaaat tcatacacaa aaacaagtgt agttttctaa
     2101 gtaaattaga ttcgacaacc aattagttta tccacatcgc attccactct tgctacacca
     2161 atcacatttc ttgatatgta atagcagaaa aaataaaacc agacaccaat agaccaaaaa
     2221 acaaacattc aacaagaaac attttgaaca atatcacaaa actgtcacaa ccatcatcag
     2281 actattgaaa aaaactggtt gctgttttat aaaagcgact gtaacaacct ccactctata
     2341 gttccattag tttaaagtgt cacacaaact gagcatccat ttgttttcct tacttttgcc
     2401 cctgtattcc aaggggtttt tttataactt aaagattagc acaccacaca aaaacaataa
     2461 aaaaaaacga caagttatac aataaaacca gtactatcca tacagctaat tacatataaa
     2521 tactattaaa ccatctggac attaaaaata aactcttata acataaaatt aatgcggttc
     2581 cggtaccata tgtcagggat aatgtgcaaa tggaggtatc ggaactgcat ttcagttcat
     2641 tattaattaa tgacaaaaaa aacctctgcg agcgcagagg gggtaaggaa tgttcccggt
     2701 cataatatgt cgatagtgtt tatatacata cagaacaaga acaacaacca gccaactaat
     2761 aaatatttta ttttttagag ctgaaacctt aaagctcact tccattccat acattgttat
     2821 aaaccttatg ttgaaaactg aaagtaagta cggcagactt attaagttgc gtgcagtctt
     2881 ttcacgttat acaaacaaga acacctgcga agttatttct ccttacatat ccccctatgc
     2941 caacggggga ttttttctgg ttcactaacg cataaataat agtgacaccc tatcattcac
     3001 aaaccgtgat aatataagga ttatcatcat ggcaaaatta ggtgaaaaca ccccacatat
     3061 tatcgatgct actgtagatt ttatggcatc aagccaggca tttcgtgagt atctgaaaaa
     3121 acttccccct gataacacaa taccgccagc tcttactgat gagcacgtac cgttatatat
     3181 acgaaggctg gaatactatc gtctgcttta tcgaccaaag cagacagaat catattgaaa
     3241 aaaacggtga acggttcgcg aaattctttc gcgggtcgtg aggcgttgga caagcgaagc
     3301 gcgtcagttt ttccggctct tccccgtgac aggtttctcc accagtttat cttccagtgt
     3361 cacaactttc tgatacaccg ccgctgtggc gttgtagttc ctgtcgagta ttgttttcat
     3421 tccccatccg agcacacaga acataaccag aaaagccact gacaccccac ccagccattt
     3481 ccagctatac cctttaaggg tgttgtattc ctgcacgata ttgctgtgaa ttgtcgcaat
     3541 atccctgttc agggcatccg cgtttgcctt catgtttgcg actgactctt ccagccgctg
     3601 aaaatcctgg ctgatgcgct cacctatcag tcctgccacg tccagcttct gcacctcatt
     3661 tctgacttca tccctgacct gttttgccac accgtcagaa atatcccgat atccgcgaag
     3721 agtttccctt gccacagcca gagaatcagc aacagctttc tgttgctcca ttgcggtatg
     3781 ttgcagatgc tccaccgata acaggagcgt aagtaatttt tcttctgtgc tggccatgtt
     3841 cagaatccca tagaaaatcc acgtgacggt gatttaaccc gttcaggtgc agttcggttc
     3901 tgtctgctca ctctgtctct catgtcaaag ccatgctccc gcgcaacatc atgttgcagc
     3961 cttctgacca gttcatgttg cttaaccgga gtgtccagcc attccctgat gtcatgcccg
     4021 accgttcgcg cctgttcaag atggcgggaa tagtccggtt cagaccgttt ttgcatgatg
     4081 tcatgacgct gggcatcatc aagccgtccg gcagcttcaa aacccagatg cacttcggga
     4141 cgtcgttcaa tgccctgttc tttcagcgtc cggcagtcaa tgaagcccct ttcaaagcca
     4201 tgctttctca tatgttcgtt gtgcttcacc tcccagcgtt ttcgcaattc ccgcaactgc
     4261 tctttttgct gagtgagagt caggcttccg gaatcctttt tcgcacctcc ccgctcggga
     4321 tacctggcgt tatagcgccg gaaatactgt tccggtgtac gttcaatgcc atcattcaca
     4381 cgctgactca tcatgatatg cgcatgaggc tgttcgccgc cgtcaatgct cgctttcgga
     4441 ttgtgaatgg caaaactcca ggcgtgccgt tcgccagctt cctgacgtac aaaatcctgc
     4501 accagttcaa ggcgctgttc cggtgtcagt tcacggggta atgcaatttc aagttcccta
     4561 taggtcgaac cgttcgcacg ttcaaactgg tcagccgcct gccagaaatg ggaaggatta
     4621 tccctggccc attccggcat gtttccgtgt gcggaatatt ccaaatcctg acggtcgcgg
     4681 tatttctctt tgcgctcgat gtaatcggcg tggtttgcag cctgcccctt gccaccaaat
     4741 ttaacgctga ggtgataggt tgccacggtt tcagccctct gcaggagcgc actgtttctc
     4801 gacgaaggag agaaacatat aagtgcgctt ccttctaatt ttacgaatta gcacggaaga
     4861 taccccctgc acccgccgac cgcaagcgat atactgcacg accacaacac accgcaaaaa
     4921 a
//
