LOCUS       NZ_CP018996             4073 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_AZ153 plasmid pECAZ153_4, complete
            sequence.
ACCESSION   NZ_CP018996
VERSION     NZ_CP018996.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511160
            Assembly: GCF_002012245.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002012245.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:51:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,216
            CDSs (total)                      :: 5,103
            Genes (coding)                    :: 4,891
            CDSs (with protein)               :: 4,891
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 73 of 212
            Pseudo Genes (incomplete)         :: 159 of 212
            Pseudo Genes (internal stop)      :: 39 of 212
            Pseudo Genes (multiple problems)  :: 50 of 212
            Pseudo Genes (short protein)      :: 1 of 212
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018996.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_AZ153"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pECAZ153_4"
                     /geo_loc_name="China: Beijing"
                     /lat_lon="39.90 N 116.41 E"
                     /collection_date="2013"
                     /collected_by="AstraZeneca global surveillance study of
                     antimicrobial resistance"
     gene            join(3674..4073,1..302)
                     /locus_tag="BE940_RS00005"
                     /old_locus_tag="BE940_00005"
     CDS             join(3674..4073,1..302)
                     /locus_tag="BE940_RS00005"
                     /old_locus_tag="BE940_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(560..2707)
                     /gene="mobQ"
                     /locus_tag="BE940_RS00010"
                     /old_locus_tag="BE940_00010"
     CDS             complement(560..2707)
                     /gene="mobQ"
                     /locus_tag="BE940_RS00010"
                     /old_locus_tag="BE940_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            2886..3140
                     /gene="mobC"
                     /locus_tag="BE940_RS00015"
                     /old_locus_tag="BE940_00015"
     CDS             2886..3140
                     /gene="mobC"
                     /locus_tag="BE940_RS00015"
                     /old_locus_tag="BE940_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(2350..2708)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2709..2885
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2..302
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.9600000000000003e-156"
     Typing          3675..4073
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.5"
                     /coverage="98.0"
                     /evalue="4.82e-174"
     Typing          complement(564..2794)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.989"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcgtgaagaa cgtctccagc ctttccagca cctacagcat ccgcatttac gagctgctcc
       61 agcagttccg cagcaccggt gacagaacga tagctatcga tgatttccga accatgcttg
      121 ccgttgaaga taaatatcaa caattcaaag acttgaataa gatgataatc aagccagcca
      181 tcgccgaact gaacgaaaaa agcgatctgg ttgtaactgt tgagacgatc aaaaaggggc
      241 gcacggttgt agccctgcat tttcgcttca aggaagacaa acagatcaag atgacgatct
      301 aaaatcgatt ttaaggcacc taaaacgctc cctgctgcgt tttttttgtg ttagtagtgc
      361 gaaggtacta ttgaagtgaa aaattgcgtc aaaaacgctc tgatgcagcg aaaaaagacc
      421 ccccaatttt tgggggcgga aaaacaaaac tatgcttgcg ttatgtgagt gtgcttcttg
      481 cattcatccc tgtcgatgaa catcgaagaa catcgacgaa cataacacga tgttaaacaa
      541 aacaaaatat tgttcacatt cactgtgacc aaccgcgatc atgtctcctc tcggtggtgt
      601 gtgttcgtgc atcttcctcg cgctgccatt cctcccaggt acgattaaaa cccagcgagc
      661 ggaacagatg atccggctgt ttttctggtg tgttgacggc agcaaaaagc ggtgcctcac
      721 gggttttcag atgaaactgc atcagttctg gtgcaggtga tatgtggctg tctgcatcgt
      781 gatttacggc aacaccatac cctgcatacc gtaacccctt atcggtcagc gcactgatga
      841 ccagatgcct gttatcggct gagggaacgg caaacgcggg atacagcgta ccattaagcc
      901 ggataacacc gcccagagag tcatccacgc ccatgaccag caaaagctga agctggcggg
      961 tttcccggtc gacagaactc atgaccggca tcagcgtcgg tggcagacgt ccctgcataa
     1021 aatccacctt ccttgccgct ggtgatggca tggctggctg gcggtcttct ccaagacaga
     1081 aagactccgg ccatgtcttc ggctggcgga gattgcgggt atgttgcggc catgtctggc
     1141 aaacctgcgt cagcagttgc tcaaaggttg cagaggaata aacacgttgc agtggcggac
     1201 gttggccgat ttcgcttcgg tcacagatat aaccactcct gtcacgcatt aaatcaacac
     1261 tctcaaagat tctggtgcgg tatttctcca gctcgttccc ccagagcgta tattctccgt
     1321 tccggttgtg aagcagaagg taacaggtca tttccccgtt accaggcatg ggagcctcgc
     1381 cataccccgt aacgactgct ctcagcaccc ctgaatccat aacggcattg tcatttttcc
     1441 tgagctgctc cgcgcgacta cgttcagtat cttcctgttc cttccggcgc aattcctctt
     1501 cacgtttaag caactgggct ctgagagctg caaggccgga ttccccccac tgctgccact
     1561 gtttttcaaa tgccatctga ctcgcgggaa tgtcctgttt ctccaccttc cggtaatgct
     1621 gtcgtaattt ttccatttgc tggctcagag actggatttt tccctgctca ctacggtatt
     1681 ccggtgccgc ccattgcatc aggggaatac gggaaaaacc ggacgaaaac ggcgatgatt
     1741 ttttcaggct ggcagcatgg ctcatcgctt cctttcgttc ggtgttcagc tcttcccagc
     1801 gtctgcgcgt cgcctgcata tctgccatca tcgacatggc attactggtg agtttccatg
     1861 ccgagttact gtccttcggc aacgcttcga taatgtcacg tagtgtgtac gaagttgcgt
     1921 tgtcaggaag cccgatattc agaaggtcag ccacacggcg ggcaggctct gtcagccctc
     1981 cgttcctgag ttcggtgcgg gaaatctgta tttccaggtt aagcctgtta atcagacgat
     2041 tctgttcgcc gcgttctgtt tcgataccgc gtgtttccat cgcacaggca gactttccga
     2101 ggtgaatacc aggcgttttc tcaagcccct gtcgctcgta tgaacgatgg tctatctctt
     2161 cctgataacc ggcgttcgcc agtgccctgt ttgcatggtc agcccatgat gcccgccacg
     2221 tctcggcatg tgtccggtcg ttccagtccc tgacctttcc gctgaaccca gcaggtccga
     2281 tagccctcgt ggtcagcatg atgtgcgcat ggggatttgt tttatccatg tgatggaacg
     2341 ccacatcagc aatcatgccc tgactgacaa agttttctct gacaaaggcg agaacggttt
     2401 cccgtgcggc atcctgcggc agttcgtggg ggatggcgag ttcaatttcc cgcgcaagtt
     2461 gtgagttctt ccggcgttcg gccttctcaa cggcattcca gagttcggca cggttcgtac
     2521 accaggcagg ggcggtggcg ggggtcagaa tcacagcctc agccacaccg cttttgcggg
     2581 tgtagtcgtg agtcagtccg gtacgctcat caagcatcag tgaaccggaa cgataggcag
     2641 cggaagctac ggcgctgtag ccgctgcttc tggagataat tttcatgctg agatgataaa
     2701 tcgccatttc tgccgtaatg cctttttgta gttttctttc agccccgcag ggcgcacaca
     2761 atcgaaacgg agtggagatt gtataagtgc gcacttcgtg atttgccgtg cattatgcca
     2821 gctcagtttt taaactacca gtgacgcgct aaaatgcgat ttttcatcgc gcacggagtt
     2881 acatcatgag cagaacgctc gaacagaaga ttgctgatgc tgaggcgaga ttacagcgcc
     2941 tgaaggcgaa gagccggagc ctggacacag cgcagaaggt tgttgtgggt gctgcattgc
     3001 tggcaaaggt cagaaagccg gaggaggtgc agttgcgtgc ctggttactc cagttcctga
     3061 aggcagaggt gacacgacag gctgatgtga cgcgcatact gccgctgatt aacgagctgg
     3121 aggcgctgcc agaacagtga ttgtatcgtc cgctcgccgc agccccagcg accgagcgtt
     3181 agcgagcgag tgggcgagga agcggaagtg aatcagtcgc caggttgagc acatacgcac
     3241 ggtttttcgg caggtgatat gcgctgaacc cgcatgaata ctgggtttcc acagggttgg
     3301 agggggtgta ttcgaaactt gcaattctgc tttattgcaa cgcattgttt ttattggttt
     3361 tttgttttca gcaccccaag ttatccacag gctcgccgtt aagtttttaa gatcttattt
     3421 ttagatcttt ttttttaaaa cctttttagg tcttgtaaca ccatgatttt aaagatgttc
     3481 tggcaagata agtggagctt ttgggggtga aaggtggagc ttttgggggt gaaaggtgga
     3541 gcttttgggg gtgaaaggtg gagcttttgg gggtgtaatc cacagaaaaa gggggttgca
     3601 aaaaaaaaac ttccacccat atgataagtg gcgtacagta aaaaaacgcc accaatagca
     3661 acgagcaagc gaaatgtcag atctgatagc ttacaaatca aacgccctta tcgaggcaag
     3721 ctacaagctc actttgcagg aacagcgctt cctgctggtc tgtatcagcc gcctaaaatc
     3781 cggagaagat tccccttcgc cggatgaaca aaaaaccatg accatcacgg ctgcagagtt
     3841 tcatgatgcc tttcctgaca tgggacggca gcacgctgaa gctaggctca aggaagcgat
     3901 agatcgcctc tgggacaggt cagtaatcat caagaacgag gaaaaacgag aagagttccg
     3961 ctgggtgcag taccgggcgc agtatttcaa aggtgaggcg aaagtagata tcaccttctc
     4021 cgatgccatc atgccgtatc tgacacagct caaggggcag tttacgcggg tag
//
