LOCUS       NZ_CP018987             6994 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_AZ146 plasmid pECAZ146_4, complete
            sequence.
ACCESSION   NZ_CP018987
VERSION     NZ_CP018987.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511152
            Assembly: GCF_002012085.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002012085.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:49:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,795
            CDSs (total)                      :: 5,681
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 283
            CDSs (without protein)            :: 283
            Pseudo Genes (ambiguous residues) :: 0 of 283
            Pseudo Genes (frameshifted)       :: 103 of 283
            Pseudo Genes (incomplete)         :: 208 of 283
            Pseudo Genes (internal stop)      :: 51 of 283
            Pseudo Genes (multiple problems)  :: 66 of 283
            Pseudo Genes (short protein)      :: 1 of 283
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018987.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_AZ146"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pECAZ146_4"
                     /geo_loc_name="Italy: Pisa"
                     /lat_lon="43.72 N 10.40 E"
                     /collection_date="2012"
                     /collected_by="AstraZeneca global surveillance study of
                     antimicrobial resistance"
     gene            join(6684..6994,1..13)
                     /locus_tag="BE932_RS00125"
                     /old_locus_tag="BE932_00115"
     CDS             join(6684..6994,1..13)
                     /locus_tag="BE932_RS00125"
                     /old_locus_tag="BE932_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            222..>656
                     /locus_tag="BE932_RS31590"
                     /pseudo=""
     CDS             222..>656
                     /locus_tag="BE932_RS31590"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            691..1251
                     /locus_tag="BE932_RS30520"
     CDS             691..1251
                     /locus_tag="BE932_RS30520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            1258..1491
                     /locus_tag="BE932_RS30525"
     CDS             1258..1491
                     /locus_tag="BE932_RS30525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001554706.1"
                     /translation="MTELEMHLLNALEQLQQDYMQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1974..2396)
                     /locus_tag="BE932_RS00145"
                     /old_locus_tag="BE932_00135"
     CDS             complement(1974..2396)
                     /locus_tag="BE932_RS00145"
                     /old_locus_tag="BE932_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001255518.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            2518..2796
                     /locus_tag="BE932_RS00150"
                     /old_locus_tag="BE932_00140"
     CDS             2518..2796
                     /locus_tag="BE932_RS00150"
                     /old_locus_tag="BE932_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(2788..3078)
                     /locus_tag="BE932_RS00155"
                     /old_locus_tag="BE932_00145"
     CDS             complement(2788..3078)
                     /locus_tag="BE932_RS00155"
                     /old_locus_tag="BE932_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            3319..4971
                     /gene="cka"
                     /locus_tag="BE932_RS31085"
     CDS             3319..4971
                     /gene="cka"
                     /locus_tag="BE932_RS31085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_001554611.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSNSGVHWGGGSG
                     HGNNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNP
                     FGGVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVSNVINEK
                     QKQKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNS
                     YKNTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAE
                     SDVQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENEL
                     KNAVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIA
                     QDLAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTK
                     ISKSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVG
                     VPLGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(4968..5258)
                     /gene="cki"
                     /locus_tag="BE932_RS31090"
                     /old_locus_tag="BE932_00155"
     CDS             complement(4968..5258)
                     /gene="cki"
                     /locus_tag="BE932_RS31090"
                     /old_locus_tag="BE932_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            5288..5419
                     /locus_tag="BE932_RS00170"
                     /old_locus_tag="BE932_00160"
     CDS             5288..5419
                     /locus_tag="BE932_RS00170"
                     /old_locus_tag="BE932_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5614..5757)
                     /locus_tag="BE932_RS32045"
     CDS             complement(5614..5757)
                     /locus_tag="BE932_RS32045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(6425..6616)
                     /locus_tag="BE932_RS00180"
                     /old_locus_tag="BE932_00170"
     CDS             complement(6425..6616)
                     /locus_tag="BE932_RS00180"
                     /old_locus_tag="BE932_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001554612.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDNGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          6253..6339
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="2.47e-38"
     Typing          complement(5686..6242)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4..1598
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.436"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6577..6992
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.489"
                     /coverage="98.0"
                     /evalue="7.1900000000000015e-75"
ORIGIN
        1 ggatgatagt taagtttcat gccaggggaa aaggcggtgg cagtggtccg gttgattacc
       61 tgctgggccg ggagcgtaac cgggaagggg cgagagttct gcggggtgct cccgaagagg
      121 tgcgggaact catcgatgcc acgccgtttg cgaaaaaata cacctcgggg gttctgtctt
      181 ttgcagagca gaccctgcca ccgggagagc gtgaaagggt gatggagagc tttgagcggg
      241 tactgatgcc cggtcttgaa aaaaatcagt acagcatcct gtgggtggag caccaggaca
      301 aggggcgtct tgagctgaat tttgtcatcc cgaatatgga gctggcgagc ggaaaacggc
      361 tccagcccta ttacgaccga gcggaccgtc cgcgaatcaa tgcctggcag acactggtga
      421 atcaccatta cgggctgcac gacccgaacg caccggagaa caggcggaca cttgtcaccc
      481 cgaataatct gccgaaagcg aaacaggagg ccgcagaagc gattacgagg ggactggagg
      541 ctctttacca tgccggagcg ctaaaaacgc gtcaggatgt cactgaggcg cttacagcgg
      601 caggttttga ggttgtgagg acaaccagga gcagtatcag tatcgccgac ccggagggtg
      661 ggcgtaatct caggctgaaa ggggcgctgt atgagcaatc ttttacaaat ggggacagac
      721 ttcgagaaga aactgaaaga gcgtgccgtc tctacagaga acatgctgaa cagcgagttc
      781 aggaagctcg aagcgtctgt agacagggaa ctgaacttaa acgggcagaa aatcagagac
      841 gctatcagcg cacacacagc agcggtgaaa gaacaactgg agacgctgaa cagtgccgtg
      901 aacgcgcagt tcagcgccac agagaacgaa ctggcccggc ggcaggaaga gctgctctgg
      961 aagctggtca aagggcggat actgtacccg tcgctgacgg cgctttgcgt cacgggcggg
     1021 atttttctgg cgtcatgggg gctgattcag tggcaggaaa gccggattgc ggcgaacatc
     1081 ctggctatca gggaccagga agagacgctg gcgaaactcc gggaaaaaac atggggtgtg
     1141 acgtatcacg agggcaggaa cgggaaattt ctagtgttgc cgtccggggt gaagggggaa
     1201 aacaactgga cggtggagaa gaagaacgca gtaagactgg tcagggagta agtgagtatg
     1261 acagagctgg aaatgcactt actgaacgcc ttagagcaat tgcagcagga ctatatgcag
     1321 cggctgaaag actgggagag cgccttcacg gatttgcagc atatgttcga gctgacacga
     1381 caggagaacg ggcaactgag ggaacagtgc gacgacttga gtcagcaggt gcagagatta
     1441 gccgggcagg tggaccactt gagtcggtta tttatcacga acagcagata agggatgaac
     1501 aggaaaggca ggcacgcctg gaacgggagc gtgcgcggtc ctggcatgaa gagcaggaac
     1561 gggaagcacg ggtatggcgt ggcccgtcaa taggtatgtg acggtgagtt gttccgggag
     1621 gttgccagat ggtaccggaa cgcgttaaaa acgcatccgg gcgcgattct ggcgtgtcgt
     1681 ttttgttgct gtgttttgcc tctttgccag atcgcgctca ttcctcagcc tcatcccgcg
     1741 atgtctgcgt atgtcatcaa ctgaaaagat ggaaaaaaac agtgtcatga ggtttacgca
     1801 ggcaaaacca gcgttattca catggctgaa tagctatgca tacggttaaa atttatcagg
     1861 tgcgatcgcg gcagcttttc gggtggtttg ttgccatttt tacctgtcag cagccgtgat
     1921 cgcgctgaac gcatttcagc ggtgcgtaca attaagggat tatggtaaat cccttactgt
     1981 ctgccctcgt atccatcgag ataaaccgca gaaatggtgt ctgccattag tttcattttt
     2041 ttcggcgaag gcatatcgct gtttgtgtat tttctgccag caaaggctgt tgtgctccgg
     2101 agtgagtgaa aaaatttttc tgagtggaat tcttttacac gttgctgtgc cgtttctgct
     2161 gatactccgg ctgctttgtc tttcatcccc atatcccgga actgctgaaa gaacgggagc
     2221 atcatacgta tggtgtctgc tttctgggtg tagaagttag gatcaaatcc atcatctgaa
     2281 gcataaacat aatgccgggt atgtcgttcc aggctggtct ttggtgtgca tcctgtaaga
     2341 gctattgttg ctgttatcag aggtatcaga agcagcctgg cagcggcaga tctcataaac
     2401 atctccttgc gtttttaatc ttgatactca gggagttata tctccgaaga gtatctctta
     2461 tcatacgtcg gtttactccc aatgtgattt gatggtcaat gagtcaggaa taatcccgtg
     2521 aaaaaatgtg tgccagtcct gctttttagt gttctttctg cttcggttgc cagtcatgcg
     2581 gacagcaatg tttttcaaca aaaggccgag aagacaaaaa acggcattgt tgcggatgtt
     2641 aatgccagac agaaaatgtt cagtaataga ctgaagatac aacttcagat cgatgagttg
     2701 agagcagagc tggctggcac cagagatgcc caaaaaagag aggatttgcg taaaaaactg
     2761 gagtctctgg agcggcaaaa gaaatcctta cgctgatggg ggcgtatttt ccccggtttc
     2821 ttccataacc tttttaccgt tcagatattt acgggcgagg cgaatgcgtc ttgcctggta
     2881 taccgccgag taagcctggg tgggattcac accattactg atttcactga ccacatgacg
     2941 ctgatcagcc agtgtcagcg tgtaaacctg tttctggatg aggtcacggg ctttgtcatg
     3001 ctcatatccg gcagcaacaa gggtgtcgag aagtcgtttg tctgttttgt tcagtttatg
     3061 gcggggtgat ttgctcacat cgttctccgt tttttcatga tccaacagtt ttatatctca
     3121 tcctgatttg ctccggtgtt ctgtttcgac gttctcaatg cctgcctccc catagtgtga
     3181 tccttattgc aaaaatgaaa atccatgctc ttgacatgga caatgctgag tagtaggttt
     3241 ttactgtaca taaaaccagt ggttatatgt acagtatatt gtttttaatt tattgtttta
     3301 attatcaaag aggaatttat ggctaaagaa ctaagtggat atggaccaac tgctggtgag
     3361 tctatggggg gaacaggggc gaatctgaat cagcaaggtg gaaataataa tagtaatagt
     3421 aatagtgggg ttcactgggg tggtggctct gggcatggta ataatggtgg tcagggaaat
     3481 tcaaattcat ctggttctac atcaactgtg atgaaaaccg gtgagtcata tctgactccg
     3541 tggggtgatg ttgtcattaa taatgatggc cttccggtga tgaatggtat tgttatgaca
     3601 gaagagaatt caactctggt tgataatcca tttggagggg tctccagagt tcttaattct
     3661 ttaattagtg atatgccatc actttttgca gaaagctcag gaaataacaa caataacact
     3721 gcttcagtta atactgcacc aacgaatgct caggtcagtg atatggataa gagttcgaag
     3781 gtggtgagta atgtaattaa tgaaaaacaa aagcagaaga ataaaattgc tacacagatt
     3841 agtgaaaaac agaagaaaat cgaggagatg aaaaaagtat tcaagcatca ttcttatcat
     3901 ggtatcactg atcttgagag ggatgttgat gagttgcaaa aaaaatcaaa tcagcttgat
     3961 gcagatatta gcaaacttaa ttcttataaa aatacattgc aaagtaaaat tggtgatgtg
     4021 aataaacaga aggaagctga agaaaaggct cgtgaaaatg cagaggtcgc tgaacatgaa
     4081 actcttaatg aagagaaaca ggcggtggcg gaagcagaga agcgtttagc tgaggcgaaa
     4141 gctgaactgg cgaaggctga aagtgatgta cagagtaagc aagctactgt ttccagagtt
     4201 gcaggagagc ttgaaaacgc tcagaagagt gttgacgtga aggtcaccgg atttccggga
     4261 tggcgtgacg ttcagaaaaa actacagcgt cagcttgaag ctaagcaggc tgagtactcc
     4321 gcagttgaaa atgaacttaa gaatgctgtt agtttcaggg atggtaaggc cgctgaggtt
     4381 aaagaggcag agcagaaatt aaaagaagct caggatgctc ttgaaaagag ccagataaaa
     4441 gatgctgttg atactatggt tggattttat caatatataa ccgaacaata tggagaaaaa
     4501 tacgcaaaaa ttgctcagga tttagcggaa aagtctaaag gaaagaaaat tcaaggtgtt
     4561 gatgaagctc tggcagcatt tgaaaagtat aagaatgtac tggataagaa attcagtaag
     4621 gttgatcggg atgctatttt taatgcgtta gagtctgtta attatgatga gttatctaag
     4681 aatttaacga agatatcaaa gtcgcttaaa atcaccagtc gagtatcttt tctatatgat
     4741 gttggttctg attttaaaaa tgcaatagaa actggtaact ggcgaccttt gtttgtcacg
     4801 ctggagaaaa gtgcggtcga tgtaggtgtg gcaaaaattg tggcattaat gtttagtttt
     4861 attgttggtg tgcctcttgg tttctggggg attgcaattg tcacaggtat tgtttcttct
     4921 tacatagggg atgatgagtt gagcaaactt aatgagttgt tagggattta atttctcttt
     4981 agagtgtaat atatgaaact tggcaatcct actgggattg ccattaataa ataaaacaat
     5041 gcagataact tgcataagta ccagggttct ttttttaact ttgtattttc tttgatatat
     5101 ctagatatgg ttaatttaga gtatggatat agcaatacat gtattgatga tataaatata
     5161 aataacaaag gagtgttatc attgtcgtta aatattaaaa taagaatcca tggtataagt
     5221 gattcaggta aattatgtag gtagtatttt aaatgcataa gtatttttac ttttgttgga
     5281 aagaaatatg aaaataataa tttttctaat tacaatatta ttcttgtcag gatgtcaagc
     5341 aaactatatt cgtgatgtac aaggaggaac tgttgcacca tcttcgtctt ctgaactgac
     5401 ggggatcgcg gttcagtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5461 gtaatctgct gcctgtaaac gaaaaaaccg cctggggagg cggtttagtc gaaggttaag
     5521 tcagttgggg actgcttaac cgggtaactg gctttagtgg agcacagata ccaaatgctg
     5581 tccttccagt gtagccgcta taaggcttca acttcaagag ttctgcgtat ctaaatccac
     5641 tatccagtta ccaatggctg ctgccagtgg cgttaagtcg tgtcttaccg ggttggactc
     5701 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5761 gcccagcttg gagcgaacga cctacaccga actgagatac caacagcgtg agctatgaga
     5821 aagcgccacg cttcccaaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5881 aacaggagag cgcacgaggg agccaccagg gggaaacgcc tggtatcttt aagtcctgtc
     5941 gggtttcgcc acctctgact tgagcgtcgg tttttgtgat gctcgtcagg ggggcggagc
     6001 ctatggaaaa acgccagctg cgtggccttt tccgggttct tgttttttgc tcacatgttt
     6061 cttttggtgt tatcccctga ttctgtggat aaccgcatta ccgtctttga gtgagctgat
     6121 accgctcgcc gcagccgaac gaccgagcgg agcgagtcag tgagcgagga agcggaaaag
     6181 cgcctggacg agcattttct ccttacgcat ctgtgcggca tttcacaccc ggcatggtgc
     6241 acttttcata caatcctcac tgatgccaaa tgattaagcc agtatacact ccgctatcgc
     6301 tacgtgactg ggtcatggct gcgccccgac acccgctaac acccgctgac gcgccctgac
     6361 gggcttgtct gctcccggca tccgctcaca ggccagctgt gaccgtctcc gggagctgca
     6421 tgtgtcagag gttttcaccg ttatccccga agcgtgcgag gcagctgcgg taaagctcgt
     6481 cagcgtgatc gtgaagcgct tcgcagatgt cagcctggtc gtcggcgtcc agttcattca
     6541 gtttttccag cagggtcagc gtctggcttc tgatgaagcg ggccatgtta agggcggttt
     6601 tttcctgttt agtcacagtt ctgtttctcc atgtgtgggg agtctgtcgg aggtttttaa
     6661 tgataccggt gaaacgcgaa aggatgctca cgatacgggt cacggatgag gaacatgcgc
     6721 ggctgctggc acgttgtgag ggaaaacaac ttgcgtcatg gatgcgtaag gtctgcctgg
     6781 gggcaccgcc atcgaaaacg tcaggtcttc cgacactggc accgccgctg ttgcgccagt
     6841 tcgcttcggt cggcaataac ctgaatcaga tagcccggaa gataaacagc ggacagtggt
     6901 cggggcatga ccgggttcat gtggtggctg cactgatggc catcggacgt gaactgagtg
     6961 agctgaggga tgaggtccgt aaacagggag aacg
//
