LOCUS       NZ_CP018986             6075 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_AZ146 plasmid pECAZ146_5, complete
            sequence.
ACCESSION   NZ_CP018986
VERSION     NZ_CP018986.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511152
            Assembly: GCF_002012085.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002012085.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:49:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,795
            CDSs (total)                      :: 5,681
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 283
            CDSs (without protein)            :: 283
            Pseudo Genes (ambiguous residues) :: 0 of 283
            Pseudo Genes (frameshifted)       :: 103 of 283
            Pseudo Genes (incomplete)         :: 208 of 283
            Pseudo Genes (internal stop)      :: 51 of 283
            Pseudo Genes (multiple problems)  :: 66 of 283
            Pseudo Genes (short protein)      :: 1 of 283
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018986.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_AZ146"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pECAZ146_5"
                     /geo_loc_name="Italy: Pisa"
                     /lat_lon="43.72 N 10.40 E"
                     /collection_date="2012"
                     /collected_by="AstraZeneca global surveillance study of
                     antimicrobial resistance"
     gene            complement(93..236)
                     /locus_tag="BE932_RS00090"
                     /old_locus_tag="BE932_00080"
     CDS             complement(93..236)
                     /locus_tag="BE932_RS00090"
                     /old_locus_tag="BE932_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001560790.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSTAELTGVE
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(298..561)
                     /locus_tag="BE932_RS00095"
                     /old_locus_tag="BE932_00085"
     CDS             complement(298..561)
                     /locus_tag="BE932_RS00095"
                     /old_locus_tag="BE932_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_001560791.1"
                     /translation="MELKNSISDYTEAEFVQLLKEIEKENVAATDDVLDVLLEHFVKIT
                     EHPDGTDLIYYPSDNRDDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     gene            complement(563..2293)
                     /locus_tag="BE932_RS00100"
                     /old_locus_tag="BE932_00090"
     CDS             complement(563..2293)
                     /locus_tag="BE932_RS00100"
                     /old_locus_tag="BE932_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E7"
                     /protein_id="WP_021530049.1"
                     /translation="MSGGDGRGHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGHGNGGGNSNSGGGSNSSVAAPMAFGFPALAAPGAGTLGISVSG
                     EALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTNVQ
                     VSTLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGV
                     PSLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPVYVS
                     VTDVLTPAQVKQRQDEEKRLQQEWNDAHPVEVAERNYEQARAELNQANKDVARNQERQA
                     KAVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDV
                     NNKKAAFDAAAKEKSDADVALSSALERRKQKENKEKDAKAKLDKESKRNKPGKATGKGK
                     PVNNKWLNNAGKDLGSPVPDRIANKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFSRNN
                     NDRMKVGKAPKTRTQDVSGKRTSFELHHEKPISQNGGVYDMDNISVVTPKRHIDIHRGK
                     "
                     /name="colicin E7"
     gene            complement(2514..3047)
                     /locus_tag="BE932_RS00105"
                     /old_locus_tag="BE932_00095"
     CDS             complement(2514..3047)
                     /locus_tag="BE932_RS00105"
                     /old_locus_tag="BE932_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031944069.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQRLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFSVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(3050..3976)
                     /locus_tag="BE932_RS00110"
                     /old_locus_tag="BE932_00100"
     CDS             complement(3050..3976)
                     /locus_tag="BE932_RS00110"
                     /old_locus_tag="BE932_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_023148976.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKGEYAKRKDLEYTEHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQDRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            4153..4551
                     /locus_tag="BE932_RS00115"
                     /old_locus_tag="BE932_00105"
     CDS             4153..4551
                     /locus_tag="BE932_RS00115"
                     /old_locus_tag="BE932_00105"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001560793.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(4755..5669)
                     /locus_tag="BE932_RS00120"
                     /old_locus_tag="BE932_00110"
     CDS             complement(4755..5669)
                     /locus_tag="BE932_RS00120"
                     /old_locus_tag="BE932_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_032333168.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPEFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGRSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     BGC             1..6075
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4895..5669)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.396"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(3054..3976)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.104"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcttccacat ggaatttttg tgacacttca acaaaaaaag cgcgtcagaa tcagcctgac
       61 acgctttgtt cttgtcaaat gaggattgca ggttactgcg tttccactcc ggtcagttca
      121 gcagttgacg acggtgatac tgtcccgccc tgaacatcac ggatatagtt tgcctgacat
      181 gcagcaagaa taatggctgc aagaagcaat aaaataatcc ctgttatttt tttcataacg
      241 actccttgtt gtgaaagaat gccagtcatt catccttaaa caaccggcat tcatatttta
      301 gccctgttta aatcctggct taccgttagc agctcgccat tctttaattt ccttgacaat
      361 cccttcgggg ctatcgtctc tattatcact aggataataa atcagatccg ttccatctgg
      421 atgctcagta atttttacaa agtgttcgag taacacatct aacacatcat cagttgcagc
      481 aacattctct ttttcaattt ccttaagaag ttgaacaaac tcagcctctg tgtaatcact
      541 aatactattt ttcagttcca tattatttac ctcggtgaat atcaatatgc cttttaggtg
      601 ttaccacgct gatgttatcc atatcataga caccaccatt ttggctgatc ggcttctcat
      661 gatgaagctc gaatgaagtt ctcttccctg aaacatcctg ggttctagtc ttgggcgctt
      721 ttccaacctt cattcgatca ttattgttcc tactaaattg tttacttaac tcaggatctt
      781 ttgacacttc ttcccagaat ttcttacgaa aatcatcgaa acttttaaac tccttatcac
      841 gtagtttatt agctatacga tctggaacag gagaacctaa gtctttacct gcattattta
      901 accacttatt attgacaggt tttcctttac ctgttgcctt ccctggctta ttccgtttac
      961 tctccttatc taatttggct ttagcgtcct tttctttatt ttctttctgt ttgcggcgct
     1021 ccaacgcaga actcaatgca acatcagcat ctgacttttc ttttgcagca gcatcaaatg
     1081 cagccttctt attatttaca tccgtctgtg cccgctgggc cttaagccct gccatttgcc
     1141 acattctgtg accagcagcc attggttctc gggcaaatcg ctcgaattgt tttatttcag
     1201 cctttgcatc agcaagagtt ttattcgctg catcaagttc acttttacga gaattataaa
     1261 cctgaacagc tttagcctgt cgttcctggt ttctggcaac atctttatta gcctgattca
     1321 gttctgcgcg ggcctgttca taattgcgtt cagccacttc caccggatgt gcgtcattcc
     1381 attcctgctg gaggcgtttt tcctcatcct gacgttgttt tacctgtgct ggggtaagaa
     1441 catctgtcac tgatacataa accggcttct gcccactttc tttcggaaag cgaatcaccg
     1501 cttcatgtga accaccacca aaggtaaatc ccgcaggaac ggcagtccgg cctttatcct
     1561 ccgtaatacc acggggaagg gttgttgaca ccggaaggcc tttgacagtg ctgactgtca
     1621 gagaagggac accaggaaat gatgcgtgaa atacacctgg agtacgtgtt ggtttggcat
     1681 tcacaacagg cacactcata ggcacacctg caactacggc aatatgctgt cgtgtgtcct
     1741 tcacaacatc tgtcacacgt ttcgtcacgc tgacagttgc ctggtccagc ggaagtgtac
     1801 tgacctgaac attcgtcact gtttctgccg gtaatgacgt cacaatcttt gacatcatat
     1861 tcgggtcatc ttttgctatt tcagatggca gaatgccgta aagcgcaata ccccatgcgc
     1921 tgaatttaaa cggacctttc agggctgcaa agatatccgc tattgccgca gataaagcct
     1981 caccagatac ggaaatacca agagttccgg caccaggagc agccaaagca ggaaaaccaa
     2041 atgccatagg tgctgctacg gatgaattgc tgccaccacc ggaattacta ttccccccgc
     2101 cattgccatg gccggagcca cctccccagt gaacaccact accggaaccg ccaccccatg
     2161 ggttattttc cgaactccat ccggagccgt cagaagcccc accatttcca ccaagccccg
     2221 tagggccgcc attaatgtta ccacctgtgt tatgtgcgcc actgttgtgg ccacgtccat
     2281 ctccaccgct cataaaaatt ccctctttta agcgttaaaa caattaatta catagttaat
     2341 actgtacata aaaccactgg ttttatgtac agatcaaaaa gtactcgcca ttattttctc
     2401 tgtcaagtca gggagggata ttttttggca gttcggagga gtcactcatg gaaacggtga
     2461 acggttcgcg aaatcctttc gcggtccgtg aggcgttgga caaacgaagc gcgtcagttt
     2521 ttccggctct tacccgtgac aggtttctcc gccagtttat cttccagtgt cacaactttc
     2581 tgatacaccg ccgctgtggc gttgtagttc ctgtcgagaa ttgttttcat cccccatccg
     2641 agcacactga acataaccag aaaggccact gacaccccac ccagccattt ccagctatac
     2701 cctttaaggg tgttgtattc ctgcacgata ttgctgtgaa ttgtcgcaat atccctgttc
     2761 agggcatccg cgtttgcctt catgtttgcg actgactctt ccagccgctg aaaatcctga
     2821 ctgatgcgct cacctatcag ccctgcaaca tccagcctct gcacttcgtt tctgacttca
     2881 tccctgacct gttttgccac accgtcagaa atatcccgat atccgcgaag tgtttccctt
     2941 gccacagcca gagaatcagc aacagctttc tgttgctcca ttgcggtatg ttgcagatgc
     3001 tccaccgata acaggagcgt gagtaatttt tcttctgtgc tggccatgtt cagaatccca
     3061 tagaaaatcc acgtgacggt gatttaaccc gttcaggttc agttcggtcc tgtctgctca
     3121 ctctgtctct catgtcaaag ccatgctccc gcgcaacatc atgttgaagc cttctgacca
     3181 gttcatgttg cttaaccgga gtgtccagcc attccctgat gtcatgcccg accgttcgcg
     3241 cctgttcaag atggcgggaa tagtccggtt cagaccgttt ttgcatgatg tcatgacgct
     3301 gggcatcatc aagccgtccg gcagcttcaa aacccagatg cacttcggga cgtcgttcaa
     3361 tgccctgttc tttcagcgtc cggcagtcaa tgaagcccct ttcaaagcca tgctttctca
     3421 tatgttcgtt gtgcttcacc tcccagcgtt ttcgcaattc ccgcaactgc tctttttgct
     3481 gagtgagagt caggcttccg gaatcctttt tcgcacctcc ccgctcggga tacctggcgt
     3541 tatagcgccg gaaatactgt tccggtgtac ggtcaatgcc atcattcaca cgctggctca
     3601 tcatgatatg cgcatgaggc tgttcgccgc cgtcaatgct cgctttcgga ttgtgaatgg
     3661 caaaactcca ggcgtgccgt tcgccggctt cctgacgtac aaaatcctgc accagttcaa
     3721 ggcgctgttc cggtgtcagt tcacggggca gcgcgatttc cagttcccga taagtcgaac
     3781 cgttcgcacg ttcaaactgg tcagccgcct gccagaaatg ggaaggatta tccctggccc
     3841 attccggcat gtttccgtgc tccgtgtatt ccagatcttt ccgtttcgca tactcccctt
     3901 tgcgctcgat gtaatcggcg tggtttgcag cctgtccctt gcctccaaat ttgacgctga
     3961 ggtgataggt tgccacggtt tcagccctcc gcaggagcgc actgtttctc gacgaaggag
     4021 agaaacatat aagtgcgctt ccttctaatt ttacgaatta gcacggaaga tacccccctg
     4081 cacccaccga ccgcaagcga tatactgcac gaccacaaca caccgcaaaa aacaccaggg
     4141 agcaccacga acatgcgcat cagcaacatc gaatggctga agaaacggat cggattcatc
     4201 aggaagctcg gagaacagac cgcacgacag agacagatta ttgacctgct cgacaacgaa
     4261 gcaggactca cagaacagga acgcaaactc ctccacgtac tggcaacagc ggaaaaaaac
     4321 gacttacagg cacaggagag cgaacgaaaa caggcggtcc aaaaacgaat cgagggaaaa
     4381 aaacaacgac gggaacgcaa tcaccggctc tttctcgcgg ctgggctact gatcgaagca
     4441 ggactggtag acacaaaaac aggcgagcta tgctataaaa aagacaggat attacaggca
     4501 cttaaggaac ttaagtacga tctggagaca tccccaaacc cagacgcgta agtgcctcca
     4561 tccgtgttcc gattccctgg tgcttcctcg ctcattcgct cgctacgctc ggtcatgaga
     4621 ctgcggcgag cactaccagc gcaatcatgc ggaaaacagc accattgcag ggctgaacgg
     4681 ggaaaaacgg caaaaaacct gctgctcaaa aagacgccaa aaaggcgctg ttatctgata
     4741 taggcttatt tggtctactc tacgagacct cgttttttca gttggtaata ccaggcgcga
     4801 ctgatcccta atgcctccca cggtttcagc gtgcgtgcag atgtagccac tgtgctgcgc
     4861 ttagacttag aaccgccctt gcgaccgcgt gcagcctgaa tttctggtgt gtgcgtatca
     4921 gccacatact gtgcgaaagt ttccggcgtg aagttcctgt gcgtgtattt cgcaatgctc
     4981 ctgccaatag cccgacattc aggcggtgaa agcggaacgg gaagcgatgc gttgtacatt
     5041 tcgacacgct gaatcacggc atcaagccac tgtgagaatt caggccagcc ctgacgaatc
     5101 gccctgtaag cccatttgcg cgtcatttcg aacaggtggc agtttcgccc catcccgtaa
     5161 tgtttatcga tgctacggcg cgctgaggcg ctcaaatcga gataatcagc cagttcatcg
     5221 agggtatagg cttcctctcg ccactccatc acctgccatt ccaggtggaa cggattttta
     5281 caaatcagtc cgctgtagtt cacatcagca caaagtttct cacacagcga acgttcaatc
     5341 gcagcggcgt atttcagcgc cttaaccgag ctgtccggcg cggttctcac ggcgatattg
     5401 agcgcgtaga gcaggtgagc atgaccgttc acaggatttt ttacggtgat gttcggcgcg
     5461 ggagcgttcc ggtcgctcca gtcaatcgct gccccctcgc ggtcaacgtc aaaaaccagc
     5521 cagaactgcg catgaggctg gttctgctgt atgtaccgcg caagaagcgc acgccctttg
     5581 ccggagatgc gaagaccaaa agcgagatca tccgtgtgat agggacggtg gggtaaattt
     5641 tcgtcgaagt attgaagcgc ggcgctcatg tgttacgatt ctctccgggt tatgcgacct
     5701 acacgccctc tgtatttcat gttgaatctt gccggatctg gacatgattt caggggcgtt
     5761 tgtttttcag tcgcttacac tacacccaac ggcaaatcct gtcgaacctt cccgaaaacc
     5821 cgatccattt accataactc ccgttaaacg caccgctaaa acgcgctcaa tcgctttctg
     5881 acgcaaaaaa cgcaaaaaga caaggaaaca ccaggctgaa ccagaaaaat gcgtcagaat
     5941 cgtttttagg ggcctgcata actatgcacg aaagcgatgc gcgatcgatt tcgactgttc
     6001 cgaagggcga acaggtcaaa atgacaagtt ttctccctcc ggatggaagt ttctgcacgg
     6061 ggattttaga tccca
//
