LOCUS       NZ_CP018985             5631 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_AZ146 plasmid pECAZ146_6, complete
            sequence.
ACCESSION   NZ_CP018985
VERSION     NZ_CP018985.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511152
            Assembly: GCF_002012085.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002012085.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:49:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,795
            CDSs (total)                      :: 5,681
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 283
            CDSs (without protein)            :: 283
            Pseudo Genes (ambiguous residues) :: 0 of 283
            Pseudo Genes (frameshifted)       :: 103 of 283
            Pseudo Genes (incomplete)         :: 208 of 283
            Pseudo Genes (internal stop)      :: 51 of 283
            Pseudo Genes (multiple problems)  :: 66 of 283
            Pseudo Genes (short protein)      :: 1 of 283
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018985.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_AZ146"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pECAZ146_6"
                     /geo_loc_name="Italy: Pisa"
                     /lat_lon="43.72 N 10.40 E"
                     /collection_date="2012"
                     /collected_by="AstraZeneca global surveillance study of
                     antimicrobial resistance"
     gene            complement(346..1212)
                     /locus_tag="BE932_RS00030"
                     /old_locus_tag="BE932_00030"
     CDS             complement(346..1212)
                     /locus_tag="BE932_RS00030"
                     /old_locus_tag="BE932_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(1214..2266)
                     /locus_tag="BE932_RS00035"
                     /old_locus_tag="BE932_00035"
     CDS             complement(1214..2266)
                     /locus_tag="BE932_RS00035"
                     /old_locus_tag="BE932_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001053268.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            complement(2747..2980)
                     /locus_tag="BE932_RS00050"
     CDS             complement(2747..2980)
                     /locus_tag="BE932_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2987..3517)
                     /locus_tag="BE932_RS00055"
     CDS             complement(2987..3517)
                     /locus_tag="BE932_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3555..4253)
                     /locus_tag="BE932_RS32040"
                     /pseudo=""
     CDS             complement(<3555..4253)
                     /locus_tag="BE932_RS32040"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4195..4518)
                     /locus_tag="BE932_RS00065"
                     /old_locus_tag="BE932_00055"
     CDS             complement(4195..4518)
                     /locus_tag="BE932_RS00065"
                     /old_locus_tag="BE932_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4583..4774
                     /locus_tag="BE932_RS00070"
                     /old_locus_tag="BE932_00060"
     CDS             4583..4774
                     /locus_tag="BE932_RS00070"
                     /old_locus_tag="BE932_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(4869..4950)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(5407..5594)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(2644..4205)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaagtggctg ccggagtgcg gctacactag aagaacaatt ttggtgactc gtctcgcata
       61 agacagttac ctggttaagc agtctgcaag ctgacttaac cttcgatcaa accgccctgc
      121 aaggcggttt tttcgtttac aggcagcaga ttacgcgcag aaaaaaagga tctcaagagg
      181 atcctttgat ctttttgtta caaacgtctc tatttgactc ccctgagagc gttttagcgc
      241 gcctctgacc cccttggtta cattccgaca ccagaaacgc tcccgttttt tacgtgcatt
      301 tggcacggta tcgatggagg ccggggggca atcctgaaca gaatgttact cctctgagta
      361 ttttctgtta agaaaatata cacttagcac agttacaata aatcctaccc cctgtaagaa
      421 tgtgaattgt tcatccaaaa acaaccatga caaaaataat gcaactagcg gaataaagta
      481 caacattatc cctgccaaat gaactgggag ccaacgcaag gagttcaagt aaaaacaaaa
      541 agctaaaaaa tattgtaata ttccagatga aaaaacaata atcacggatt ccaacggaag
      601 ataaattgat gatttagata taaaaataaa gagcgatgac agtataaggg agaatgtcaa
      661 ctgtccgctc aataatgtga taggctcaaa cgatttaata aggcgagacg atataacaac
      721 ataaaatgct gccgataaca caccggcaac aacaagacaa tatccaacaa tattattcga
      781 tccattactg atgtctggca aggctaccaa aatactgcca acaattgcac ctataaggaa
      841 caatataaaa tattgcgggg aattaatttt aactgagcaa ataagaacag acaacaccaa
      901 tataaacact gattcaagtg aaaaaataat tgatgcgctt cctgctgaaa taaaataaag
      961 tccgtagagt gtaaaagtat acgctaagaa tggttcaaaa acgcctgtcg cataagcaag
     1021 tatcgctttt gagtttaatg ggagtttttt tctttttata aaaagaacta tccaagatag
     1081 tattactgca ctacaaatct gccagaaaaa cacccctccg gcatctagtt cttcaactcc
     1141 ttttttgctc attacgatag ctgcccccca tgagaacgct gctaaaaaag caaaaagcat
     1201 tcctttaatc atattaatca tcctctttct cataaagggg tttgttttca aatttccttg
     1261 gttgaaagga gttaaaaaac atagatgcat catctacagg aataccaata aactctccat
     1321 aataaggatc atctatagga aactcttcta atgaaacata aaacaataca ttccagttga
     1381 agatcattgg atcactattt tctatttcat ggagcaatct ttcatcctta gttaattctc
     1441 ctggaattaa tacatcagta tgataaaaac cgctgtaata agaactatta ccggtgtgca
     1501 ctgggcatcc aaaacgatcc aaattagatt ctgaatgcca tgttgtagaa atcccttccg
     1561 gatgtgaata cccaccctta ccaacagact gaatagtcat gttacgaata tccgctaacg
     1621 cttgttcttg atctttatag aaaccttttt ctatcgccag cctcataaaa tgccccgggg
     1681 catttacaga aataagtatt ccccctggta ttcttctatc atgatttctt gttctatgag
     1741 cagaagagta aaaaacatta acaccagcct tatatatttt tagcccatct ttatcctgaa
     1801 atggtacgct ttcagtataa ataacatcct cttttattgg atagcactct ggcatatgca
     1861 gcgaacaaat atatttttgt atgtccacta actcttctga tggacgagca aaggcaagtt
     1921 gtggatgaat aaaataaatg agatgcgcac tgacctctcc tcttcttgcg cgttctttaa
     1981 aacgaactct ttcattacgc aaaaaatcaa acaaatgacc atcatcatta tatagttggg
     2041 ggctatttac aatagataaa tggaaatcaa gccctttaat tttcttgctt gccaatttac
     2101 caaatacaca gggctgattg cctgacaccc attttcttag taaatcttca attttatctt
     2161 tagaagtttc gttaaagata aatgaatgaa tattatcaat atcttttcca aatgaaaaac
     2221 tggatgataa ttgtggtagt ttatttatac gttgttgaag attcatgaaa gctcccacaa
     2281 gacacctcat atattttttt atatactgaa actacattat atttattacc ggcgtaaaac
     2341 gaaataattt attatcgtta ccgatatttt aaaaaaatca ttatttgcac agtgttcgag
     2401 ttgttagtat cactccttcc tgaaaatccg ctccatttac cataacgtcg cattgtacgc
     2461 accgccaaaa cgcgttcggt gcgatcacgg cagcagacag gtaaaaatgg caacaaacca
     2521 cccgaaaaac tgccgcgatc gcacctgata aattttaacc gcatgaatac ctatgcaacc
     2581 agatgctaca ggccacatta gcccgcctta atccactgaa cacgtcattt ttcatggttt
     2641 caccatccca gcgaagggcc atccagtgtc cgctcctgga tttccggctg acgctcccgc
     2701 tccagagata actcatgttc gcgttcctgc atcagccgtt catcccttat ctcctgttct
     2761 cgctgtataa ctggctcaag cgttctgtcc gctcgctcaa gtgctgcacc tgctgactca
     2821 actgcatgac tcgcccgttc agcatcgcgt tgtcccgttg cgtaagcgaa aacattttct
     2881 gcaattccac gaaggcgttc tcccattcgt tcagccgctg catatagtcc tgttgcagtt
     2941 gctctaatgc gttcagcaaa tgtctttcca gctccgtcat gctctgtcac tcctgcacca
     3001 gtctcactgc gttcagtttg ccgttgtcct tcgtccagtt cgtttcggct ttcatcccct
     3061 tcggcaacac cagaaaacgt ccgttgctgt cctcgtgata acgcacgccc catgtcttcg
     3121 cgttcagctt ctccagactc tctttctgct cgcggagaat attcatattc gcgtttatct
     3181 tcatgccctg gtaccacagc agactcccgg tcgtggcaaa catcagtact gagacgataa
     3241 tcagccacag ccagcgtttc acgctcattt tcagtacggt tttccagtgc gcgtccatcg
     3301 tatcgacggt cgttttctga tacctctcca gcagttcttt cagggctgtt gtatggtcgt
     3361 tgatagcgtt cctgattaaa gattcgtttg aggtcagttc cctgtttaca gattcttcga
     3421 gcttcctgaa ctcgtcgttc agcattttct ctgtagattc ggctctctct ttcagctttt
     3481 tctcgaactc cgcgcccgtc tgtaaaagat tgctcataaa acgctccttt cagtctgata
     3541 ttcctcccgc cgttcgggtc tgcaatgctg atactgcttc gcgtcaccct gaccacttca
     3601 agccccgcct ctgtgagcgc ctgaatcaca tcctgacggc cttttatctc tccggcatgg
     3661 taaagcgcgt ctatcccgcg tgtgacgccc tcagcaagcg cctgtttcgt ttccggcagg
     3721 ttatcaggaa gggtcagcgt ccggcggttc tccggggcgt tggggtcatg cagaccgtaa
     3781 tggtgattca ccagcgtctg ccaggcatca attctcggcc tgtctgcccg gtcgtagtac
     3841 ggctggaggc gttttccggt ctgtagctcc atgttcggaa tgacaaaatt cagctcaagc
     3901 cgtcccttgt cctggtgctc cacccacagg atgctgtact gatttttctc gagaccgggc
     3961 atcagtacac gctcaaagct cgccatcact ttttcacgcc ctccaggcgg cagctccttc
     4021 tccgcgaacg acagaacacc tgacgtgtat ttcttcgcaa atggcgtggc atcgatgagt
     4081 tcccggacct cttccggatt cccctgaagc accgttgccc cttcccggtt acgctccctt
     4141 cccagcaggt aatcaaccgg accactgcca ccaccttttc ccctggcatg aaacttaact
     4201 atcatcccgc gccccctgtt ccctgaccgc cagacgcagc cggcgcagct catccccgat
     4261 ggccatcagt gcggccacca cctgaacccg gtcaccggaa gaccactgcc cgctgttcac
     4321 cttcctcgct gtctggttaa ggttattccc gatggcggcc agctgacgca gtaacggcgg
     4381 tgccagtgtc ggcagcttcc cggaacgggc aaccggctca cccaggcaga cccgccgcat
     4441 ccataccgcc agttgtttac cctcacagcg ttccagtaac cgggcatgtt catcatcagt
     4501 aacccgtatc gtgagcatcc tctcgcgttt catcggtatc attaccccca tgaacagaac
     4561 ccccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg
     4621 cttcatcaga ggccagacat taacgctgct ggagaagctc aacgaactgg acgcggatga
     4681 acaggcagac atctgtgaat cgcttcacga ccacgccgat gagctttacc gcagttgcct
     4741 cgcacgcttc ggggatgacg gtgaaaaccg ctgacacatg cagctcccgg agacggtcac
     4801 agcttgtctg tgagcggatg ccgggagcag acaagcccgc cagggcgcgt caggccgttg
     4861 acctttgggg tgtcggggcg aagccctgac ccagtcacgt agcgatagcg gagtgtatac
     4921 tggcttaacc attcggcatc agtgcggatt gtatgaaaag tgcgccatgt ccggtgtgaa
     4981 atgccgcaca gatgcgtaag gagaaaatgc cagttcaggc gctcttccgc ttcctcgctc
     5041 actgactcgc tacgctcggt cgttcggctg cggcgagcgg actctccttt ctcaaaaacg
     5101 ctaaccctgt tatccacaga acccggggat aaaccaggaa agaacatgtg cgcaaaaaac
     5161 gaaaccccgg gaaaggccgc gccggaggcg ctttttccat aggctccgcc cccctgacgg
     5221 acttcacaaa acagacactt tttcggtgca tctgtgggag ccttgcggct caaccatgaa
     5281 atccgccagc ggtggcgaaa cccgacgtgg cttaaagatc atcaccgttt ccggtgaatc
     5341 gctccctcct gcgcccccac gttcccgcca tatcgattac cggatacctt ttccgccttt
     5401 ctcccttacg ggacacgtgg cgctttctca tagctcacgt cttctggtat ctcggggcga
     5461 cttaggcagt tcgctccggg ctgggctgta tgcaggaacc ccccgttcag cccgactgct
     5521 gcgccttctc cggtaactgt tctcttgggt ccaacccgga aaagacacca caaagcgcca
     5581 ctggcagcag ccattggtaa aagtcatgtg cgagatattt agagagtact t
//
