LOCUS       NZ_CP018984             2571 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_AZ146 plasmid pECAZ146_7, complete
            sequence.
ACCESSION   NZ_CP018984
VERSION     NZ_CP018984.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511152
            Assembly: GCF_002012085.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002012085.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:49:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,795
            CDSs (total)                      :: 5,681
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 283
            CDSs (without protein)            :: 283
            Pseudo Genes (ambiguous residues) :: 0 of 283
            Pseudo Genes (frameshifted)       :: 103 of 283
            Pseudo Genes (incomplete)         :: 208 of 283
            Pseudo Genes (internal stop)      :: 51 of 283
            Pseudo Genes (multiple problems)  :: 66 of 283
            Pseudo Genes (short protein)      :: 1 of 283
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018984.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_AZ146"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pECAZ146_7"
                     /geo_loc_name="Italy: Pisa"
                     /lat_lon="43.72 N 10.40 E"
                     /collection_date="2012"
                     /collected_by="AstraZeneca global surveillance study of
                     antimicrobial resistance"
     gene            complement(join(2529..2571,1..395))
                     /locus_tag="BE932_RS00005"
                     /old_locus_tag="BE932_00005"
     CDS             complement(join(2529..2571,1..395))
                     /locus_tag="BE932_RS00005"
                     /old_locus_tag="BE932_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001557684.1"
                     /translation="MNTQNLIAEHREKHDRDNLGFTQVYPLGWKRLMALAKGNQGAFGL
                     YTFLAQHIDGACGAVVADQAFLADQFDVTTRTVRNWISYLEEQKAIVKIPVAGRVCAYA
                     LNPHEVWKGYDNNKPTAAFLTKTLVSKDGEIKRRIMAMFSN"
                     /name="hypothetical protein"
     gene            complement(1641..1853)
                     /locus_tag="BE932_RS30510"
     CDS             complement(1641..1853)
                     /locus_tag="BE932_RS30510"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128936982.1"
                     /translation="MRPLTENELKNVSGALYESVNTNCVLASGAMIVSAFGGPIGLIST
                     GLAAIGACATSSSSSSASASAGNGS"
                     /name="hypothetical protein"
     gene            complement(1873..2190)
                     /locus_tag="BE932_RS00025"
                     /old_locus_tag="BE932_00025"
     CDS             complement(1873..2190)
                     /locus_tag="BE932_RS00025"
                     /old_locus_tag="BE932_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V family bacteriocin"
                     /protein_id="WP_021574241.1"
                     /translation="MREITLNEMNNVSGAGDVNWVDVGKTVATNGAGVVGGAFGAGLCG
                     PVCAGVFAVGSSAAVGALYDAAGNSKPTKQKPEGLPPEAWNYAEGRMCNWSPNNLNDVC
                     L"
                     /name="colicin V family bacteriocin"
     Typing          complement(795..879)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
ORIGIN
        1 tctttgctga ctaaggtctt tgttaagaat gcagctgttg gcttgttatt gtcatagcct
       61 ttccacacct cgtgtgggtt aagagcatag gcgcaaactc ttcccgcaac gggaattttg
      121 actatggctt tctgctcttc taggtacgag atccaattgc gtacagttcg agtggtcaca
      181 tcaaactgat cagccagaaa agcctgatcc gctacaacag caccgcacgc accatcgata
      241 tgctgtgcca aaaaagtata caagccaaat gcgccctgat ttcctttggc aagagccatc
      301 agccgcttcc atcctagtgg atacacttga gtgaatccga ggttatctct gtcatgtttt
      361 tctcgatgtt ctgcgattag attctgggta ttcatagcga ttcctatcaa ccggaaatta
      421 tcattcctat agtaggaaat ttattttcca catcaacgct tatttcattc ctactttcag
      481 aagtgatagg aaacacatgt gtttcctatc atgcggaaat acatgtgttt cctatcaggc
      541 tacatttcta taaataaaaa caacaactta catgttccgc taattgttat cttaagagca
      601 ctatgtttcc taatgcacga tcgtttggtt ttggtttttt tggggtttcg gggcgaagcc
      661 ctgaaccagt cacgcagcga tagcggagtg tatactggct tactcctctg caagcctcgt
      721 cagccgtgat ttttgtccga tttcgtgctc tccacgttca acagctccat catgtttttg
      781 ccgtccgttc accgctaaaa cggctcagaa atcggaacag gatgtgggaa tgtcggaccg
      841 cgcacgcgca ctcacacatt ccccacctgg tcagggcttc accccgacac cccgcatcag
      901 tgcgtaagtg ctctatgtgg cttccggttc tcttccgctt cctcgcccac tcgctcacta
      961 cgttcggtcg ctggggctgc ggcgagcggt gtcacctctc gcatgttgtc agcgattctg
     1021 ttgctgttct gttcgaaaat gcgttctgta agccatccag agcaccagaa cgcgattttc
     1081 tggtgtctgt aatggttggg tacatattgg cccttaaaac gcgctcagaa gctcccagac
     1141 gcacgaaacg gggtttaaat gcccgctagg gcatggaagg cgcgaaaaac gcttacagag
     1201 cgtttcagga ggtcaggcgc gccgataccc ctaaccaggg gctttcaggt cgccaggaac
     1261 cgcgccagcc ccctgcaaag cccatttgca cggaacagac agccactcgg ctggtaggtg
     1321 gtcgtagacc gtcgatgttg cagcgatggg ggtctggggt tctccccaga cggtttccgg
     1381 ttgcgctcaa ggcgctgccg gaaccgtgaa agttatttag ctgactgaat tcgtcttatt
     1441 ttcaaaagaa gaattctctc ccatataatt aatattcctg taaagaatat taaccagtct
     1501 ttattaggta gctgatataa aatgaacatc aacattacag aaataatagt tgtaaccatg
     1561 atattcacgt attctttcga gaaaaatata gatttaagtt ctaacttaat caattcgtcg
     1621 aacatgaata ctcctaatag ttaagatccg ttccctgcag aagcactagc cgaagatgac
     1681 gatgatgaag tggcgcaagc acctatggct gctaatccag tagatattaa acctataggt
     1741 ccaccgaaag cagatactat cattgctcca gaagctaaaa cacagttagt attaacgctt
     1801 tcatacaaag caccagatac atttttcaat tcattttctg ttaatggtct cattaaataa
     1861 ttccttttca tattataaac aaacatcatt aagattattt ggactccaat tacacattct
     1921 accttcagca tagttccatg cttctggtgg cagtccttct ggtttttgtt tcgttggttt
     1981 ggaatttcct gctgcatcat acaacgcacc aacagcagca gaagatccga cagcgaacac
     2041 accggcacaa acagggccgc atagccccgc tccgaacgca ccgccgacca caccagctcc
     2101 gtttgtagct acagtttttc cgacatcaac ccaattgaca tcaccagcac cagacacatt
     2161 attcatttca tttagcgtta tttctctcat acgtcactta tctcctatta acaaaaatag
     2221 atatcataaa tgcaatagcg ataccgctaa ctatagactg aacaaggttg ctagcattta
     2281 gctgtttcgc aacaagacta ataataatgc ttatgggtaa tataatgaag aatacttgcc
     2341 acgttttcat ttatattcct atgcttaatt tatagtaacg ttcacaatac gatcgttcat
     2401 ttcatattaa aagttgtata tataaaaata tgatttgggt aacaatttta ttaccattgg
     2461 ataaatgcag gcagacaatc atggtcaaaa cgttaacacg tagccctgat tgtctgcctg
     2521 ccgctggctc aatttgaaaa catggccata atccggcgct taatttcccc a
//
