LOCUS       NZ_CP018981            14029 bp    DNA     circular CON 14-JUN-2024
DEFINITION  Escherichia coli strain Ecol_867 plasmid pEC867_KPC, complete
            sequence.
ACCESSION   NZ_CP018981
VERSION     NZ_CP018981.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511148
            Assembly: GCF_002012205.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14029)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002012205.1-RS_2024_06_14
            Annotation Date                   :: 06/14/2024 01:50:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,987
            CDSs (total)                      :: 4,874
            Genes (coding)                    :: 4,677
            CDSs (with protein)               :: 4,677
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 197
            CDSs (without protein)            :: 197
            Pseudo Genes (ambiguous residues) :: 0 of 197
            Pseudo Genes (frameshifted)       :: 69 of 197
            Pseudo Genes (incomplete)         :: 146 of 197
            Pseudo Genes (internal stop)      :: 36 of 197
            Pseudo Genes (multiple problems)  :: 44 of 197
            Pseudo Genes (short protein)      :: 1 of 197
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018981.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..14029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_867"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC867_KPC"
                     /geo_loc_name="Canada: Toronto"
                     /lat_lon="43.65 N 79.38 W"
                     /collection_date="2013"
                     /collected_by="Merck Study for Monitoring of Antimicrobial
                     Resistance Trends (SMART)"
     gene            complement(1..780)
                     /gene="istB"
                     /locus_tag="BE928_RS00030"
                     /old_locus_tag="BE928_00030"
     CDS             complement(1..780)
                     /gene="istB"
                     /locus_tag="BE928_RS00030"
                     /old_locus_tag="BE928_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISKpn7 family helper ATPase
                     IstB"
                     /protein_id="WP_004152394.1"
                     /translation="MNLQHERIDALCQQLKLERVATHYPVLAQQAVREDFSFTDFLEHL
                     LRHEAGCRQQRSRELLTRMACFPGIKTLEDYDFSFNPGVPKALVQELGSLAFVERAENV
                     VLIGPSGIGKTHLAIALGYKAAQAGVKTRFITAADLMLQLSTAQRQGRLKEYFNRAILG
                     PRLLVIDEIGYLPFGREEANLFFQVVAKRYEKGAMILTSNLPFSQWADAFAGDTTLTAA
                     MLDRLLHHAHILTLSGESYRLKDKRKAGVVRKNSKPE"
                     /name="istB"
     gene            complement(777..1802)
                     /gene="istA"
                     /locus_tag="BE928_RS00035"
                     /old_locus_tag="BE928_00035"
     CDS             complement(777..1802)
                     /gene="istA"
                     /locus_tag="BE928_RS00035"
                     /old_locus_tag="BE928_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490293.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="WP_004199214.1"
                     /translation="MLTQEQTVEIRVLARQGHSIRHIARTLGVSRNTVRRYLRDPSVAR
                     YQPREPRPTKLGPFESYLRQRVEQAHPIWLPATVLNREIRAQGYGGGLSLLRAFLATLK
                     PARREAGPAVRFETEPGRQLQADFVVLRRARSPMSAFVATLGYSRMTFVTFVPDESFES
                     VRDSLLLAFDYLGGVPREVLFDNMKTVVLERDAYGDGKHRFHPGLLQLADDLGFRIRLC
                     RPYRAQTKGKVERFNRYFRESFYNPLLTRMKGTGLLLDCAAANRRVRDWLADEANVRVH
                     ATLNERPIDRWRQEREHLQPLPSRVRRDEAPLLDNSLRPVPLESLQHPLSVYDAIGEAC
                     R"
                     /name="istA"
     gene            complement(1909..4938)
                     /gene="tnpA"
                     /locus_tag="BE928_RS00040"
                     /old_locus_tag="BE928_00040"
     CDS             complement(1909..4938)
                     /gene="tnpA"
                     /locus_tag="BE928_RS00040"
                     /old_locus_tag="BE928_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152392.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn4401 family transposase"
                     /protein_id="WP_004152392.1"
                     /translation="MSKKNKLLSVLSDAEQEALYGLPEFDDAQQLEYLAVTETELALAN
                     SRPSLHAKVYCLLQIGYFKAKHAFFSFDWDEVEDDCAFVLSRYFHGEVFEPKAITKHER
                     YTQREQIAKLFGYWPWSAAFLPQLEQQAARTVRRDVTPGFVAAELIVWLNEHKIIRPGY
                     TTLQELVSETLSAERRRLGNLLEQALDESAKAALSQLLVRDDTLSQLAALKQDAKNFGW
                     RQMVREREKRATLEPLHAIAKTLLPKLDVSQQNLLYYASLANFYTVHDLRNLKADQTQL
                     YLLCYAWVRYRQLSDNLVDAMAYHMKQLEDESSAGAKQSFADEQVRRQQETPRVGRLLS
                     LYVDDSVADPTPFGEVRQRAYKIMPRDTLQTTAQRMSVKPASKLALQWQAVDGLADRMR
                     RHLRPLYVALDLSGSNPDSPWLAALAWAKGVFAKQQRLSQRPLDEWPAATLPKRLRPYL
                     LTFDADGKPTGLHADRYEFWLYRQIRKRLQSGELYLDDSLQHRHFSDELVEMDRKIDVL
                     AKMEIPFLQQPVHAQLDALTAELRTQWLAFNRELKQGKLTHLEYNKDTQKLTWRKPKSE
                     NEKAREKAFYEKLPFCDVADVFRFVNDECKFLSAFTPMQPRYAKKVADADSLMAVIIAQ
                     AMNHGNHVMARTSDIPYHVLDSGYQQYLRQATLHAANDCISNAIATLPIFPYYSFDLET
                     LYGAVDGQKFSVERPTVKARYSRKYFGRGKGVVAYTLLCNHIPLNGYLIGAHDYEAHHV
                     FDIWYRNTSDIMPTAITGDMHSVNKANFAILHWFGRRFEPRFTDLNAQLKELYCADDPA
                     QYQACLIRPVGKIDCDLIHREKPNIDRIVATLGLKEMTQGTLIRKLCTYTTTNPTRRAI
                     FEFDKLIRSIYTLRYLRDPQLERNVHRSQNRLESYHQLRSAIAQVGGKKELTGRTDIEI
                     EISNQCARLIANTIIFYNSAILSRLVTKYEAAGNSKALALITKISPAAWRHILLNGHYT
                     FQSSGKTIDLDAIVAGLELE"
                     /name="tnpA"
     gene            5048..6763
                     /gene="tnpR"
                     /locus_tag="BE928_RS00045"
                     /old_locus_tag="BE928_00045"
     CDS             5048..6763
                     /gene="tnpR"
                     /locus_tag="BE928_RS00045"
                     /old_locus_tag="BE928_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn4401 family resolvase TnpR"
                     /protein_id="WP_004152391.1"
                     /translation="MKTGQTTASSSTPVGFPDADSLAALRGWYAGLSSRAAVEHYLAAR
                     KAAGQSARGMLGHIRRQLIVFANSRHRPDLADVFQHAPEERQGRAGAVERAIRLLPTLP
                     APKPQISDSIDQWLSPRTVRALQAYGIHTLSALTVRIPRRRGWWKAIEGLGMTGARQVE
                     AFFAAHPQLTERARALIPIIQPSGIVPWEQLRLPNEVDGSQGNFRAPRNACTLNATNDY
                     EAVQAWLALHETVATQRAYRKEAARLILWAIVERGRALSSLTTDDAIAYRAFVRHPTPR
                     ERWVGPPRPRDSVEWRPFSGGLSARSAAYALTVLSALFRWLIEQRYVLANPFAGVKVRG
                     HALRPALDTARGFTEGEWLLLRAIADGLEWSYGWSEPAAQRLRFMLDFGYATGLRASEL
                     VGAVLGNVRLDEHGDHWLHLVGKGGKPGKVALPPLAYTALDQYLAQRQLPVSRERWNPK
                     TPLVGSLGEDGNAGITGPHLWCVLRRFFTQAAEAIEADHPVAAEKLRRATPHWMRHSHA
                     SHALARGAQLTAVRDNLRHASIATTSMYLHGDELERARQMRQAFGARHVKRGVD"
                     /name="tnpR"
     gene            complement(7175..7288)
                     /locus_tag="BE928_RS00050"
                     /old_locus_tag="BE928_00050"
     CDS             complement(7175..7288)
                     /locus_tag="BE928_RS00050"
                     /old_locus_tag="BE928_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072035093.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHDKTPLAAATGN"
                     /name="replication initiation protein"
     gene            7936..8259
                     /locus_tag="BE928_RS00060"
                     /old_locus_tag="BE928_00060"
     CDS             7936..8259
                     /locus_tag="BE928_RS00060"
                     /old_locus_tag="BE928_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024191642.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQICLDEKPSRAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQVVAVLMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            8468..>8902
                     /locus_tag="BE928_RS27315"
                     /pseudo=""
     CDS             8468..>8902
                     /locus_tag="BE928_RS27315"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8937..9422
                     /locus_tag="BE928_RS26830"
     CDS             8937..9422
                     /locus_tag="BE928_RS26830"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_061390563.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMISQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIQEQKSTQAMLSEKNNGVQLTTCGEERRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            9426..9641
                     /locus_tag="BE928_RS26835"
     CDS             9426..9641
                     /locus_tag="BE928_RS26835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_072048928.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            9770..10297
                     /locus_tag="BE928_RS00070"
                     /old_locus_tag="BE928_00070"
     CDS             9770..10297
                     /locus_tag="BE928_RS00070"
                     /old_locus_tag="BE928_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016246513.1"
                     /translation="MKCFWACSVSVVLAMSFTVAAAENNDNVTYTKPSAGRDLYLTCKG
                     YQVKGASVINVEKPSYLEGTGEQVEFAIITPVGASDGLKVFFDSWGFSSFGLIESHVMH
                     EHWSLVHVGGRASVKGEKIDYSFVDNQPQEGGSYKPKTGVFLYGCMNSDKRPSWVEKIY
                     TRTGVFRGGIED"
                     /name="hypothetical protein"
     gene            10552..10764
                     /locus_tag="BE928_RS00075"
                     /old_locus_tag="BE928_00075"
     CDS             10552..10764
                     /locus_tag="BE928_RS00075"
                     /old_locus_tag="BE928_00075"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_034169353.1"
                     /translation="MGDFERTFGAGADFDRIIDENNSEYFREDRRKTKNSKKKFSSFQD
                     ALVWAKANPGKTIIRSPDGVGFIEK"
                     /name="hypothetical protein"
     gene            11193..12512
                     /locus_tag="BE928_RS00080"
                     /old_locus_tag="BE928_00080"
     CDS             11193..12512
                     /locus_tag="BE928_RS00080"
                     /old_locus_tag="BE928_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
                     /protein_id="WP_004152397.1"
                     /translation="MAASYLPYRPDQSYLLPPSLGEWLPEGHLAYFISETVDTLDLSAF
                     HARYAGGGPGNQPFHPAMMVKVLIYGYASGVFSSRKLARKLYEDVALRVLAAGNFPAHR
                     TLSDFRALHLTELENLFVQVVQLARECGLVKLGTIAVDGTKVKANASRHKAMSYKRMKP
                     AEDELHCEIKALLDRAKATDDQERNEPELDIPAEISRREKRLEAIQAAKARLEARQREA
                     DQARGRSEDDGRRPRHPDGSDKGGGSYKREFGVPDDRDQESFTDPDSRIMKHAGGGSEQ
                     SYNGYTAVDAEHQIIVAAELTNCAADSQALLGMLAAVQANTGEMPAQTLADAGFRSEAV
                     LAKVADHHGDVIVALGREGREDAKVNAKTHPHTAAIAAKLKTEQGDAAYRRRKSIVEAP
                     NGWIKAVMGLRQFSMRGLDKVQAEWKLVCMALNLRRMAYL"
                     /name="IS1182-like element ISKpn6 family transposase"
     gene            complement(12762..13643)
                     /locus_tag="BE928_RS00085"
                     /old_locus_tag="BE928_00085"
     CDS             complement(12762..13643)
                     /locus_tag="BE928_RS00085"
                     /old_locus_tag="BE928_00085"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-3"
                     /protein_id="WP_004152396.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSIG
                     VYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALVPW
                     SPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFRLDR
                     WELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNHRIRA
                     AVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKYSEAVIAAAA
                     RLALEGLGVNGQ"
                     /name="carbapenem-hydrolyzing class A beta-lactamase KPC-3"
     ARG             complement(12766..13643)
                     /name="blaKPC-3"
                     /gene="blaKPC-3"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-3"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          7646..7823
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.222"
                     /coverage="83.0"
                     /evalue="4.5e-50"
     Typing          complement(7176..7696)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.564"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          8250..9733
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.717"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tcattcgggt ttggaatttt tcctgaccac tcccgccttc ctcttgtcct tcaagcggta
       61 gctttcgccg ctcagggtca ggatatgggc atggtgcagc agccgatcca gcatcgcggc
      121 tgtcagggtg gtatcgccgg caaacgcgtc ggcccactgg ctgaacggca ggttcgaggt
      181 caggatcatc gcccctttct catagcgctt cgccaccacc tgaaagaaca ggttcgcttc
      241 ctcgcgtccg aaaggcaggt agccgatctc atcgatcacc agcagccgtg gaccgaggat
      301 ggcgcggttg aagtattcct tcagccgccc ttgtcgctgc gccgtgctca gttgcagcat
      361 caggtcggct gccgtgatga agcgtgtctt gaccccggct tgcgcggcct tgtaacccaa
      421 ggcgatggcg aggtgggtct tgccgatgcc agagggtccg atcagcacca cgttctcggc
      481 ccgttcgaca aacgccagac tgcccagctc ctgcaccagt gccttgggca cgccggggtt
      541 gaagctgaag tcatagtcct ccagggtctt gatgcccggg aagcaagcca tccgcgtcag
      601 cagttcgcgg ctgcgctgct ggcgacaccc cgcttcatgc cgcaacaggt gttccaggaa
      661 gtcggtgaag ctgaagtcct cgcgcaccgc ctgctgcgcc aggacggggt aatgcgttgc
      721 cacccgttcg agtttgagtt gctggcacag ggcgtcgatc cgctcatgct ggaggttcat
      781 cggcaggcct ccccaattgc gtcatagaca gacagtgggt gctggagaga ttccagtggt
      841 accgggcgga gactgttatc cagcaggggg gcctcgtcgc gccgcacacg ggatggcaag
      901 ggttgcaggt gttcccgctc ctgccgccaa cggtcgatgg ggcgctcgtt cagggtggcg
      961 tggacccgca cattcgcctc gtctgccaac cagtcacgga cgcgtcggtt ggcagcagca
     1021 caatcgagca gcagccccgt gcccttcatc cgggtcagca acgggttgta gaagctctcg
     1081 cggaagtagc gattgaagcg ctcgaccttg cctttggtct gggcccgata cgggcgacac
     1141 agccggatcc ggaaccccag gtcatcggcc agttgaagca agccggggtg aaaacggtgc
     1201 ttgccgtcac cgtaggcatc gcgttccagc accacggtct tcatgttgtc gaacaacacc
     1261 tcgcggggca caccgcccag atagtcaaaa gccagcaaca gggagtcccg cacggactcg
     1321 aaggattcgt ccggtacgaa ggtgacaaac gtcatgcggc tgtatcccag cgtggcgacg
     1381 aaggccgaca tgggggatct ggcccggcgc aagaccacga aatcagcctg caactgccgt
     1441 ccgggctcgg tctcgaaacg gacggccgga cctgcttcgc ggcgggcggg cttgagcgtg
     1501 gcgaggaagg cgcgcaataa cgagagaccg ccgccatacc cttgagcgcg gatttcccga
     1561 ttcaggacgg tggcaggcag ccagatgggg tgggcctgct cgacccgttg ccgcagataa
     1621 gattcaaatg gccccagttt ggtggggcgt ggttcacggg gctggtatcg cgctaccgac
     1681 ggatcgcgga ggtagcggcg cacggtattg cgcgagaccc ccaacgtgcg ggcgatgtgc
     1741 cggatggagt ggccctgtcg ggcaagaact ctgatttcca cggtttgctc ctgggtcaac
     1801 atcgacggcc tcgaaaaggc cgccaaacta gcccaggtgg atcagtttta cttcgctggg
     1861 gtgggtcagt tttacactgc tgctaacacg gaaccgctga aaattccgtc actccagttc
     1921 caggcctgcg acgatcgcat cgagatcgat cgtcttgccg ctgctttgaa aggtgtagtg
     1981 cccgttgagc aggatgtgcc gccatgccgc aggcgaaatt tttgtgatga gtgccagcgc
     2041 cttgctgttg ccggccgcct cgtacttggt caccagccgc gacaagattg ccgagttgta
     2101 gaaaatgatc gtgttggcga tcagccgtgc gcactggttg ctgatctcga tttcaatgtc
     2161 ggtgcgaccg gtcagttcct tcttaccgcc gacctgggca atggccgagc gtagctgatg
     2221 gtaggactcg aggcgatttt gcgagcggtg cacgttgcgt tccagttgcg gatcgcgcag
     2281 gtagcgaagc gtgtagatgc tgcggatgag cttgtcgaac tcgaagatcg cgcgccgcgt
     2341 cgggttcgtc gtggtgtagg tgcacagctt gcggatcagc gtgccctgcg tcatttcctt
     2401 cagcccgagt gtggcgacga tccggtcgat gttcggcttc tcgcgatgga tgagatcgca
     2461 gtcgattttc ccgactggcc gaatcaggca cgcctgatac tgtgctggat cgtcagcgca
     2521 gtagagttct ttcaattgcg cgttgaggtc ggtgaagcgc ggctcgaacc gccggccaaa
     2581 ccaatgcagg atggcgaagt tggccttgtt gacgctgtgc atgtcgccgg tgatcgcggt
     2641 cggcatgatg tccgacgtgt tgcgatacca gatgtcgaac acgtggtggg cctcgtagtc
     2701 gtgcgcgccg atcaggtagc cgttgagcgg gatgtggttg cacagcagcg tgtaggcgac
     2761 cacgcccttg ccgcgcccga agtacttgcg cgaatagcgc gccttcacgg tcggccgttc
     2821 gacgctgaat ttctgcccgt cgacggcacc gtacagcgtt tcgaggtcga acgagtagta
     2881 tgggaagatc ggtagcgtgg cgatggcgtt gctgatgcag tcgttggccg cgtgcagcgt
     2941 tgcctggcgc aggtactgct ggtagccgct atccagcacg tggtacggga tgtcgctggt
     3001 gcgcgccatg acgtggttgc cgtggttcat cgcttgcgcg atgatgaccg ccatcaggct
     3061 gtcagcgtcg gcgaccttct tcgcgtagcg cggctgcatg ggcgtgaacg ccgacagaaa
     3121 cttgcactcg tcgttgacga agcgaaacac gtcggccacg tcgcagaacg gaagcttctc
     3181 gtagaatgcc ttttcgcgcg ctttctcatt ctcgctcttg ggcttgcgcc aggtcagttt
     3241 ctgcgtgtcc ttgttgtatt cgaggtgcgt cagcttgccc tgtttcagct cgcggttgaa
     3301 cgccagccac tgcgtgcgca gttcggcagt cagcgcatcg agttgggcat ggacgggttg
     3361 ctgcaggaag gggatctcca tttttgcgag cacatcgatc ttcctgtcca tctcaaccag
     3421 ttcatctgag aagtgccggt gttgcaggct gtcgtcgagg tagagttcac ccgattggag
     3481 acgcttcctg atctggcggt acagccagaa ctcgtagcgg tcagcgtgca ggccggttgg
     3541 tttaccgtcg gcgtcgaagg tcagcaggta cggccgcaag cgtttcggaa gtgtcgccgc
     3601 gggccactcg tcgagtggcc gttgcgacag ccgctgctgc ttggcaaaaa cacctttggc
     3661 ccaggccagc gcggcaagcc atggactgtc tggattggag ccggagaggt cgagcgcaac
     3721 gtacagtgga cgaagatggc ggcgcatgcg gtcggccagc ccgtccaccg cctgccactg
     3781 caaggccagc ttgctcgccg gcttcacgct catacgctgc gccgtggttt gcagcgtatc
     3841 gcgaggcatg atcttgtagg cgcgctgccg cacctcgccg aagggcgtgg gatcggccac
     3901 gctatcatca acgtagagtg acagcaggcg gcctacccgc ggcgtttcct gctgacggcg
     3961 cacctgctcg tcggcaaagg attgtttggc accagcgctg ctttcgtcct ccaactgctt
     4021 catgtggtac gccatcgcat cgaccaggtt gtcggagagc tgccggtagc gtacccaggc
     4081 atagcacagc aggtagagtt gggtctgatc ggccttcaga ttgcgcaggt cgtggacggt
     4141 gtagaagttt gccaggctcg cgtagtacag caggttctgc tgcgagacgt cgagtttagg
     4201 cagcagtgtc ttggcgattg cgtgcagcgg ctccagcgtg gcgcgctttt cgcgctcgcg
     4261 gaccatctgg cgccagccga aattcttggc gtcctgcttg agcgccgcca gttgcgacag
     4321 ggtgtcatca cggaccagaa gctggctcag cgcggccttg gcagattcgt ccaacgcttg
     4381 ctccagcaga ttgcccaggc gccggcgctc ggcggatagc gtttcgctga ccaactcttg
     4441 caaggtcgta tagccgggcc ggataatctt gtgctcattg agccagacga tcagctcggc
     4501 ggcgacgaac cccggcgtta cgtcacgccg cacggtccgt gcggcctgct gctcgagctg
     4561 cggcaggaag gcggccgacc acggccagta gccgaatagt ttggcgatct gctcgcgctg
     4621 ggtgtagcgt tcatgcttgg tgatcgcctt gggttcgaac acctcgccgt ggaaatagcg
     4681 gctcagcacg aaagcgcaat cgtcctcgac ttcgtcccag tcgaaactga agaaggcatg
     4741 cttggccttg aagtagccga tctgcaagag gcaatagacc ttggcatgaa gactgggccg
     4801 gctgttggca agcgccaact cggtttctgt cacagccaga tattccagct gctgcgcatc
     4861 atcgaactcc ggcaggccat acaaggcctc ctgttcggcg tcggatagga cgctgagtag
     4921 cttgttcttc ttgctcatgg cccgttcttg ctatcttgag gtttcgtaga aacatggggc
     4981 attttcgatt ctactgaaat aatcgttatt atcgcgttac tcgcttttcg aatcgcaacc
     5041 tgtccatatg aaaacgggcc agactacggc ttcgagttct accccagtcg gctttcccga
     5101 tgcggattcg ctggctgcct tgcgcggctg gtacgcggga ctcagctctc gcgcggccgt
     5161 tgaacactac ctggcggcac gcaaggccgc cggtcaatca gcgcgaggga tgctggggca
     5221 tatccgccgc cagctcatcg ttttcgccaa cagccgccac cggccggatc tggccgacgt
     5281 gttccagcac gcccccgaag agcgacaggg gcgcgcgggt gccgtcgaac gcgcgatccg
     5341 gctcttgccc acgctgccgg cgccgaagcc ccagatcagc gacagcatcg accagtggct
     5401 gtctccgagg accgtcaggg cactgcaggc gtacggtatc cacaccctct cggcgctgac
     5461 cgtgcgcatt ccccgccggc gtggctggtg gaaagccatc gagggactcg gcatgacagg
     5521 cgcccggcag gtcgaggctt tctttgccgc acatccgcag ctcaccgagc gcgcccgggc
     5581 cctgatcccg attattcagc ccagcggcat cgtgccgtgg gagcagttgc gtctgccgaa
     5641 cgaagtcgac ggctcgcagg gcaatttccg ggccccgcgc aacgcttgca cgctgaatgc
     5701 gaccaacgac tacgaggccg tgcaggcctg gctggcgctg cacgagactg tcgccacaca
     5761 gcgcgcctat cgcaaggagg ctgcgcggct aatcctgtgg gccatcgtcg aacgcggacg
     5821 ggcgctatca tccctgacca cggacgatgc aatcgcgtac cgggcctttg tccggcaccc
     5881 gaccccgcgc gagcgttggg tggggccacc tcgaccacgc gattcggtgg aatggcgccc
     5941 cttctcgggc ggcttgtccg cacgctcggc agcgtatgcg ctgacggtgc tctcggcgct
     6001 gttccggtgg ctcatcgagc agcgctatgt gctggccaac cctttcgccg gcgtcaaggt
     6061 acgcggtcac gccttgcggc ccgcactgga tacggcgcga ggctttaccg agggagaatg
     6121 gctactgctg cgcgccattg cagacggcct ggaatggtcc tacggctggt ccgagccggc
     6181 agcacaacgc ctgcgcttca tgctcgactt cggctatgcc acgggcctgc gcgctagcga
     6241 actggtcggc gccgtcctgg gtaatgtccg cctcgacgag cacggtgacc actggctgca
     6301 tttggttggc aaaggtggca aaccaggtaa ggtggccttg ccgccgctcg cgtatacggc
     6361 gctggaccaa tacttggcac agcgtcaact gccagtcagc cgggaacggt ggaatccgaa
     6421 aacgcccctg gtgggcagtc ttggggaaga cggtaatgcc ggcatcaccg gccctcacct
     6481 ttggtgcgtg ttgcgaagat tctttacgca agccgctgag gccattgagg ccgatcatcc
     6541 ggtcgccgcc gaaaagctgc gccgtgccac gcctcattgg atgcgtcaca gtcatgccag
     6601 ccatgcgttg gcccggggag ctcagctcac cgcggtgcgc gacaacctac gccatgcgtc
     6661 gatcgccacg acgtcgatgt atttgcatgg cgatgagctc gagcgcgcca ggcagatgcg
     6721 gcaggctttt ggcgctcgtc acgtcaagcg aggagtcgac tgaattttca tcatacggaa
     6781 tgacggaatt ttctgggatt cccgcttaga acccctcaca gtgtcgtatg ttggagtcta
     6841 tgttacgtac gttgcagggc ctattttgtt agttacaggc attcttgcat ggcttacgaa
     6901 acctagctgt aatccaaagt gactgggcta gacgaacttt atccccccat aaaacccgct
     6961 tttttaaacg gatcttcttg agatcattca tggagaatcg taatctctta gcactgtaaa
     7021 cgaaaaaacc gcccagcaag gcggtttttt cgaaggttct cagagcagca actctttgaa
     7081 ccgaggtaac tggcttggag gagcacagtc accaaaactt gtcctttcag tttagcctta
     7141 accggcgcat aacttcaaga ctaactcctc taaatcagtt accagtggct gctgccagtg
     7201 gcgttttgtc gtgtctttcc gggttggact caagtcgata gttaccggat aaggcgcagc
     7261 ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg
     7321 aactgagtgt caggcgtgga attagataaa cgcggccata acagcggaat gacaccggta
     7381 aaccgaaagg caggaacagg agagcgcacg agggagctac cagggggaaa cgcctgggat
     7441 ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg
     7501 tcaggggggc ggagcctatg gaaaaacggc aacgccgcat cctttttccg ccctttctga
     7561 ttgatttccg cttagtctct ttatccagcc attcgtaagc tggcaacgcc cgccgcagtc
     7621 tcacggcagg agcgcagcga ccgagtgagc gaggaggcgt catttcaccg ggatctgcat
     7681 ttcgcactta cgccccacag cagcattttc tccctgccac atgaagcact ttcctgaatc
     7741 cccactccga tttaccatgt taagccagta tacactccgc tagcgctacg tgactggttc
     7801 agggctgcgc cccgaaaccc gctgaaaagt actgccgcgc cctgcgggct tgcccaacac
     7861 cgctccggac aggaaaaaat ttcccgcccg aaccggtgtt atccggagga tggtttggca
     7921 gaaaagcgca acaaaatgct cactatgtgg gtgacagaag acgagcaccg ccgacttctg
     7981 gagcgctgtg agggtaaaca actcgcggcc tggatgcgcc agatatgcct ggacgaaaag
     8041 ccctcgcgtg ctggcaaact tccatccatc tcgccggcgc tgcttcgtca gcttgccggc
     8101 atgggtaaca acctcaacca gattgcccgg caggttaacg ccggtggcgg tactgggcac
     8161 gatcgcgtgc aggttgtcgc cgtgctgatg gccattgatg ccggactcga gcggctgcgg
     8221 cacgccgtgc tggaaaaggg ggcagacgat gatcgttaag tttcatccca gaggccgcgg
     8281 cggtggcggc ggtccggtgg attatctgct ggggaaagac cggcagcgcg acggcgccag
     8341 cgttctgcag ggtaagccgg aggaagtgcg ggagcttatt gacgcctcgc cttacgccaa
     8401 gaaatacacc tccggcgtgc tgtcttttgc cgaacaggat ttgccgcccg gccagcgtga
     8461 aaagctgatg gcgagctttg agcgggttct gatgcccgga ctcgataaag accagtacag
     8521 cgtactgtgg gttgagcacc gggacaaagg gcggctggag ctgaacttcc tgatcccgaa
     8581 cacggagctg ctgaccggca gacgtctcca gccgtattac gaccgcgccg accgtccgcg
     8641 cattgatgcc tggcagacca tagtgaacgg cagactgggg ctgcacgacc cgaacgcgcc
     8701 ggaaaaccgg cgggcgctgg ttacaccgtc cgcgctgccg gaagcgaagc aggaagccgc
     8761 ccaggcgatt acgcgcggcc tactttccct tgcctcatcc ggggagctga aaacccgtca
     8821 ggacgtcact gaggcgctgg aaagtgcggg ttttgaggtg gtgcgcacca cgaaaagcag
     8881 catcagcatt gccgaccctg acggggggcg aaacatccga ctgaagggag ccatctatga
     8941 acagtctttt aacgctggcg aaggacttag agcagaaatc gaaagcgcag cagcagacta
     9001 ccggcgagat gctgaaagcc gcattcagcg agcacgagaa gtctgtcaga gcggaactga
     9061 gcgaaagcga gaagagaatc agcgccgcca tcctcgacca cgaccggaag ctgtcctcag
     9121 ccatgagcca acgcacgaaa gggatgctgc gtatgatcag ccagacgtgg ctgaccatag
     9181 tcctggtctc cgcgctactg atcgcctcga gcgcgggcat tctgtggtgg caggggcaac
     9241 agatactcga caactacacg accatccagg aacagaagag cacgcaggcc atgctttcag
     9301 agaagaacaa cggcgtccag ctcacgacct gcggggagga gagacgccgc tgcgtgaggg
     9361 tgaacccgga agcgggaaga ttcggagagg attcgagctg gatgatactg gcggggaaat
     9421 agcacatgac ggaactggaa aaacagttgc tgagcgcatt agagcagcta cagcaggact
     9481 actcgaaaag gctggacgag tgggagaacg ccttcgcgga atggcggacg atgtctgggc
     9541 ttatgcaacg ggagaacgcg gcgctgagcg agcgcgtcac gggcttgagc aggcaggtgc
     9601 agagtttgag cgatcagctg cgccgattgt cgcaaggctg aatggcatcg aggcgcaccg
     9661 ggagcaggag cgcgcggcgc agcaccagaa agcgctggag ctggaacgat cgcagcggca
     9721 gaaaggtttt gagcttggaa tgtagttaat caatgaaaag ggatgcgtta tgaaatgttt
     9781 ctgggcttgc tcagtctcgg tggttttggc tatgtcattt acagtcgctg ctgcggagaa
     9841 taacgacaac gttacatata caaaaccgtc agccgggagg gacttatatc ttacctgtaa
     9901 aggttatcag gtgaaggggg catctgtaat taacgtcgag aaaccttctt accttgaagg
     9961 tactggtgag caggttgaat ttgcaatcat tactcctgtt ggtgcgtctg acggactcaa
    10021 ggtctttttt gattcatggg gtttttcttc atttggattg attgaaagcc atgtgatgca
    10081 tgagcactgg tctttagttc atgttggggg gcgtgcttca gtcaaaggtg aaaagattga
    10141 ttattcattt gtagataacc agccacagga gggggggagc tataaaccca aaacaggtgt
    10201 gttcctgtac ggctgcatga actcggataa aagaccatct tgggtcgaaa agatctacac
    10261 aagaacaggt gtgtttcgag ggggcataga agactaaaat actgcttaca gcatgaaaat
    10321 ccattgaaca gagtgtcagt tcatttccgt gcataactat gcataccgta agaacttacg
    10381 gggggcattt atcggcgttt ttacgtggta agaggtgctc ttttgcggtt catggcgcag
    10441 aacggtgctg aggctgtctg cggcggtgcg aacaagggat gttatggtaa ataagagtgt
    10501 cttttggata ttttaaatct gtttttttat taaatttaat gggggcagaa aatgggggat
    10561 tttgagcgta cctttggagc tggtgctgat tttgatcgga taattgatga gaataacagc
    10621 gaatattttc gagaagatcg ccgaaaaact aaaaattcaa aaaaaaaatt ctcttctttt
    10681 caagatgctt tggtttgggc gaaagctaat cctggcaaaa cgataataag atctccagac
    10741 ggtgtggggt ttattgaaaa atgaaagagt ggattcactg taaaacaaca gtcatttttt
    10801 ggacaacact gtctatatac gtactcatat gtttcacagg gggggtaagc gggaacccca
    10861 gaaaattccg ccattccgca ttgtggaatt ttttgggggg gtggtccgcg gcatgacgac
    10921 ccgccaaagg ctaccgcatc tatctgaggc gctgtggcgc gacgctgaaa ccccggggaa
    10981 ttccgctact ccagttccag gtccgcaata gttcgtccag gtcgggtggc gtacgccgga
    11041 aacgccgatt tttccgcaac cgtttctgcg ttgtcggcaa ggtggtctca gagagcgctc
    11101 aagaagcggt ttttctcgta tgtttagcta cggtggccta ggagcctgcg cggcagaagc
    11161 caaatcggcg gaagatcgag gacgatacgg ggatggctgc cagttacctt ccttaccgac
    11221 ccgaccaatc ctatctgctg cccccttctc tgggagagtg gctacctgaa gggcatcttg
    11281 cctacttcat cagcgagact gtcgatacgc tggacttgag cgcattccac gcccggtatg
    11341 ccggcggtgg tccgggcaat cagccgtttc atccggcgat gatggtcaaa gtgctgatct
    11401 atggttacgc gagcggcgtc ttctcttcgc gcaaactagc caggaagctg tacgaggatg
    11461 tcgcgttgcg tgtgctggcc gctggaaact tcccggccca ccgcacgctg agtgacttcc
    11521 gtgccctaca cctgaccgag cttgagaatt tgttcgttca ggtggtgcaa ctggcgcgcg
    11581 aatgtgggct ggtgaagctt ggcacgatcg cggtcgacgg caccaaggta aaagccaacg
    11641 ccagccgcca caaggcgatg agctataagc gcatgaagcc ggccgaggac gaattgcatt
    11701 gcgagatcaa ggcgctgctt gatcgcgcca aggctaccga cgaccaggag cgtaacgagc
    11761 cggagctgga cattcctgcc gagatttctc gccgcgagaa gcgcctggag gcgatccagg
    11821 cggcaaaggc gcgcctggaa gcgcgccagc gtgaagcgga ccaggcccgg gggcgcagcg
    11881 aagacgatgg ccgccggcct cgccatccgg atggctcgga caagggcggt ggctcgtaca
    11941 aacgcgagtt tggtgtgccg gatgaccgtg atcaggaaag cttcaccgat ccggacagcc
    12001 ggatcatgaa acacgccggt ggtggctccg agcagagcta caacgggtac acagcggtcg
    12061 atgccgagca ccagatcatc gtggcggcgg agttgaccaa ctgcgccgcg gacagtcagg
    12121 cgctgctggg catgctggca gcagttcagg ccaacaccgg agaaatgccg gcccagacgc
    12181 tggcggatgc gggattccgt agtgaggctg ttctggcaaa ggtcgccgat caccacggcg
    12241 atgtcatcgt tgccctcggc cgcgagggac gtgaagatgc caaggtcaat gccaagaccc
    12301 atccgcatac ggcggcgatt gcggcgaaat tgaaaacgga gcaaggcgat gcagcttacc
    12361 gccggcgcaa gtcgatcgtg gaggctccga atggttggat caaggcggtg atgggattgc
    12421 gtcagttcag catgaggggc ctggacaagg tgcaagccga gtggaagctc gtctgcatgg
    12481 cgctaaatct gaggcgaatg gcgtatctgt gagggcgaag gttaaatggg gcggctcaaa
    12541 tgcaccccag tcgtcataac acgccgcgcg ccgcaagatt ggtatccttg gcgccgacgc
    12601 cttgccaatt gcagagagcg ccgccgccat cgtcagtgct ctacagaaaa ccggtcacac
    12661 ggctctgccg cgcagactcc tagcctaaat gtgacagtgg ttggtaatcc atgccgcccg
    12721 caagggcggc ggtggtgggc caatagatga ttttcagagc cttactgccc gttgacgccc
    12781 aatccctcga gcgcgagtct agccgcagcg gcgatgacgg cctcgctgta cttgtcatcc
    12841 ttgttaggcg cccgggtgta gacggccaac acaataggtg cgcgcccagt gggccagacg
    12901 acggcatagt catttgccgt gccatacact ccgcaggttc cggttttgtc tccgactgcc
    12961 cagtctgccg gcaccgccgc gcggatgcgg tggttgccgg tcgtgtttcc ctttagccaa
    13021 tcaacaaact gctgccgctg cggcgcagcc agtgcagagc ccagtgtcag tttttgtaag
    13081 ctttccgtca cggcgcgcgg cgatgaggta tcgcgcgcat cgcctgggat ggcggagttc
    13141 agctccagct cccagcggtc cagacggaac gtggtatcgc cgatagagcg catgaaggcc
    13201 gtcagcccgg ccgggccgcc caactccttc agcaacaaat tggcggcggc gttatcactg
    13261 tattgcacgg cggccgcgga cagctccgcc accgtcatgc ctgttgtcag atatttttcc
    13321 gagatgggtg accacggaac cagcgcattt ttgccgtaac ggatgggtgt gtccagcaag
    13381 ccggcctgct gctggctgcg agccagcaca gcggcagcaa gaaagccctt gaatgagctg
    13441 cacagtggga agcgctcctc agcgcggtaa cttacagttg cgcctgagcc ggtatccatc
    13501 gcgtacacac cgatggagcc gccaaagtcc tgttcgagtt tagcgaatgg ttccgcgacg
    13561 aggttggtca gcgcggtggc agaaaagcca gccagcggcc atgagagaca agacagcaga
    13621 actagacggc gatacagtga catcaacgat attccttgtt tgaaggtgga gctaggtgta
    13681 gcggccggat gtaatcaggc cgctacagct gggattatca ccgagcggca agccgctccc
    13741 tctccagagg ctgtaacggc tgtaactgcc ccggatgggc acacatcggg ttggtaagtc
    13801 cctggcacca caacgtccag cgctattgaa aaacgaatca ggtcggcgtt catgggtcgg
    13861 ccacctcgcc tgacctcggc atccacgtta cggacggcct caggaagtcc tggccaagcc
    13921 ccgactattg gggcgcgaag atagcaccga gggtacgccg gtgtcaacac caactgaaaa
    13981 ctgacccact ttacccctga atcaccaaag taatactgac ccacctgga
//
