LOCUS       NZ_CP018980             4074 bp    DNA     circular CON 14-JUN-2024
DEFINITION  Escherichia coli strain Ecol_867 plasmid pEC867_3, complete
            sequence.
ACCESSION   NZ_CP018980
VERSION     NZ_CP018980.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511148
            Assembly: GCF_002012205.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002012205.1-RS_2024_06_14
            Annotation Date                   :: 06/14/2024 01:50:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,987
            CDSs (total)                      :: 4,874
            Genes (coding)                    :: 4,677
            CDSs (with protein)               :: 4,677
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 197
            CDSs (without protein)            :: 197
            Pseudo Genes (ambiguous residues) :: 0 of 197
            Pseudo Genes (frameshifted)       :: 69 of 197
            Pseudo Genes (incomplete)         :: 146 of 197
            Pseudo Genes (internal stop)      :: 36 of 197
            Pseudo Genes (multiple problems)  :: 44 of 197
            Pseudo Genes (short protein)      :: 1 of 197
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018980.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_867"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC867_3"
                     /geo_loc_name="Canada: Toronto"
                     /lat_lon="43.65 N 79.38 W"
                     /collection_date="2013"
                     /collected_by="Merck Study for Monitoring of Antimicrobial
                     Resistance Trends (SMART)"
     gene            join(3879..4074,1..497)
                     /locus_tag="BE928_RS00005"
                     /old_locus_tag="BE928_00005"
     CDS             join(3879..4074,1..497)
                     /locus_tag="BE928_RS00005"
                     /old_locus_tag="BE928_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(755..2914)
                     /gene="mobQ"
                     /locus_tag="BE928_RS00010"
                     /old_locus_tag="BE928_00010"
     CDS             complement(755..2914)
                     /gene="mobQ"
                     /locus_tag="BE928_RS00010"
                     /old_locus_tag="BE928_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_023146018.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWV
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            3093..3347
                     /gene="mobC"
                     /locus_tag="BE928_RS00015"
                     /old_locus_tag="BE928_00015"
     CDS             3093..3347
                     /gene="mobC"
                     /locus_tag="BE928_RS00015"
                     /old_locus_tag="BE928_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2557..2915)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.333"
                     /coverage="100.0"
                     /evalue="8.460000000000002e-179"
     Typing          2916..3092
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2..497
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.582"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(759..3001)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.198"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aggttcagtt acaggaagcg atcgatcgtt tatgggacag gtcgatcatt ctcaagaatg
       61 acgagaagcg tgaggagttc cgctggatcc agtatcgggc acagtacgct aaaggtgagg
      121 ccaaggctcg aataaccttt tctgacgcgg taatgcctta tctgacgcaa ctgaaggggc
      181 aattcacgcg cgttgttata aaaaacatat caggtttatc aagtagttac agtataagaa
      241 tatacgagtt cctccagcaa ttccgcagta cgggagagag aattattgct cttaatgact
      301 ttcgttcaat gttagggatt gaaaataaat acaaacaatt cagagactta aataaaatac
      361 taataaaacc ctgtgttgac gaactgaaca aaaaaagcga cctggctgta acggttgaga
      421 cgatcaaaaa ggggcgcacg gttgtagccc tgcattttcg cttcaaggaa gacaaacaga
      481 tcaagatgac gatctaaaat cgattttaag gcacctaaaa cactccgtgc tgcgtttttt
      541 ttgtgttagt agtgcgaagg tactattgaa gtgaaaaatt gcgtcaaaaa cgctctgatg
      601 cagcgaaaaa agaaccccca atttttgggg gcggaaaaac aaaactatgc ttgcgttatg
      661 tgagtgtgct tcttgcattc atccctgtcg atgaacatcg aagaacatcg acgaacataa
      721 cacgatgtta aacaaaacaa aatattgttc acattcactg tgaccaaccg cgatcatgtc
      781 tcctctcggt ggtgtgtgtt cttgcatctt cctcgcgccg ccattcgtcc caggtacgat
      841 tgaaacccag cgagcggaac agatgatccg gctgtttttc tggtgtgttg acggcagcaa
      901 aaagcggtgc ctcacgggtt ttcagatgaa actccataag ttccggcgca ggtgatatgt
      961 ggctgtctgc atcgtgattt acggcaacac cataccctgc ataccgtaac cccttatcgg
     1021 tcagtgcact gatgaccagc tggctgttat cggctgaggg aacggcaaac gcgggataca
     1081 gcgtaccatt aagccggaca acaccgccca gagagtcatc cacgcccatg accagcaaaa
     1141 gctgaagctg gcgggtttcc cggtcgacag aactcattac cggcatcagc gtcggtggca
     1201 gacgtccctg cgtaaaatcc accttccttg ctgccagtga tggcatggct ggctggcgat
     1261 cttctccaag acagaaagac tccggccatg tcttcggctg gcggagattg cgggtatatt
     1321 gcggccatgt ctggcaaacc tgcgtcagaa gttgctcaaa ggtcgcagag gaataaacac
     1381 gttgcagtgg tggatgttga ccgatttcgc ttcggtcaca gatataacca ctcctgtcac
     1441 gcattaaatc aacacgctca aagattctgg tgcggtattt ctccagctcg ttcccccaga
     1501 gcgtgtattc tccgttccgg ttgtgaagca gaaggtaaca ggtcatttcc ccgttaccag
     1561 gcatgggggc ctcgccatac cccgtaacga ctgcgctcag tacccctgaa tccataacgg
     1621 cattgtcatt tttcctgagc tgttccgctc gtcgctccgc gcgactacgt tctgtatcct
     1681 cctgctcctt ccggcgcaat tcctcttcac gtttaagcaa ctggactctg agagctgcaa
     1741 ggccggatgc cccccactgc tgccactgtt tttcaaatgc cgtctgactc gcgggaatgt
     1801 cctgtttctc caccttccgg taatgctgtc gcaatttttc catttgctgg ctcagcgact
     1861 ggatttttcc ctgttcgcta cggtattccg gtgccaccca ttgcatcagg ggaatacggg
     1921 aaaaaccgga cgaaaacggc gatgattttt tcaggctggc agcatggctc atcgcttcct
     1981 ttcgttcggt gttcagctct tcccagcgtc tgcgcgtcgc ctgcatatct gccatcatcg
     2041 acatggcatt actggtgagt ttccatgccg cattactgtc cttcggcaac gcttcgatga
     2101 tgtcacgtag tgtgtacgaa gttgcgttgt caggaagcac gatattcaga aggtcagcca
     2161 cacggcgggc aggctctgtc agcccgctgt tcctgagttc agtgcgggaa atctgtattt
     2221 ccaggttaag cctgttaatc agacgattct gttcgccgcg ttctgtttcg ataccgcgtg
     2281 tttccatcgc acaggcagac tttccgaggt gaataccagg cgttttctca agcccctgtc
     2341 gctcgtatga acgatggtct atctcttcct gataaccggc gttcgccagt gctctgtttg
     2401 catggtcagc ccatgatgca cgccacgtct cggcatgtgt ccggtcgttc cagtccctga
     2461 cctttccgcc gaacccagca ggtccgacag ccctcgtggt cagcatgatg tgcgcatggg
     2521 gatttgtttt atccatgtga tggaacgcca catcagcaat catgccctga ctgacaaagt
     2581 tttctctgac aaaggcgaga acggtttccc gtgcggcatc ctgcggcagt tcacggggga
     2641 tagcgagttc aatttcccgc gcaagttgtg agttcttccg gcgttcggcc ttctcaacgg
     2701 cattccagag ttcggcacgg ttcgtacacc aggcaggggc tgtcgcgggg gtcagaatca
     2761 cagcttcagc cacaccgctt ttgcgggtgt agtcgtgagt cagtccggta cgctcatcaa
     2821 gcatcagtga accggaacga taggcagcgg aagctacggc gctgtagccg ctgcttctgg
     2881 agataatttt catgctgaga tgataaatcg ccatttctgc cgtaatgcct ttttgtagtt
     2941 ttctttcagc cccgcagggc gcacacaatc gaaacggagt ggagattgta taagtgcgca
     3001 cttcgtgatt tgccgtgcat tatgccagct cattttttaa actaccagtg acgcgctaaa
     3061 atgcgatttt tcatcgcgca cggagttaca tcatgagcag aacgctcgaa cagaagattg
     3121 ctgatgctga ggcgagatta cagcgcctga aggcgaagag ccggagcctg gacacagcgc
     3181 agaaggttgt tgtgggtgca gcattgctgg caaaggtcag aaagccggag gaggtgcagt
     3241 tgcgtgcctg gttactccag ttcctgaagg cagaggtgac acgacaggct gatgtgacgc
     3301 gcatactgcc gctgattaac gagctggagg cgctgccagg acagtgattg tatcgtccgc
     3361 tcgccgcagc cccagcgacc gagcgttagc gagcgagtgg gcgaggaagc ggaagtgaat
     3421 ccgtcgctag gttgagcaca tacgcacggt ttttcggcag gtgatatgcg ctgaacccgc
     3481 atgaatactg gatttccaca gggttggaag gggagtattc ggaacttgca attctgcttt
     3541 cttgcaacac attgttttta ttgttttttt gttttcagca ccccaagtta tccacaggct
     3601 cgccgttaag tttttagatc ttgtttttaa gatcttgttt ttaatacttg ttttaggggt
     3661 gaaattcatt aataaaatca atatgttata gaggtatatg tggaggaatt taaggtaaag
     3721 gtggaggaat ttaaggtgtt taggtggagg aatttaaggt aaaggtggag gaatttaagg
     3781 tgttattcac agaaatgtga ggatataaaa aaaaatattc cacatttctt atttgtgggg
     3841 cacattaaag catcaccaca tttgaacggg atctttgtat gtcggacttg attgcctaca
     3901 agtcgaatgc tttggttgag gccagctaca agttgaccct acaggaacaa cgttttctgt
     3961 tgctgtgtat cagtcgattg aaatctggtg ctgatgctga attgcaaaag acaatgacca
     4021 ttaccgctgc ggaatatttt gattcattcc ctgacatggg gcggaaaaat gccg
//
