LOCUS       NZ_CP018972             4073 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_545 plasmid pEC545_4, complete
            sequence.
ACCESSION   NZ_CP018972
VERSION     NZ_CP018972.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511169
            Assembly: GCF_002012045.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002012045.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:48:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,033
            Genes (coding)                    :: 4,867
            CDSs (with protein)               :: 4,867
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 62 of 166
            Pseudo Genes (incomplete)         :: 110 of 166
            Pseudo Genes (internal stop)      :: 30 of 166
            Pseudo Genes (multiple problems)  :: 31 of 166
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018972.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_545"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC545_4"
                     /geo_loc_name="Viet Nam: Ho Chi Minh City"
                     /lat_lon="10.82 N 106.62 E"
                     /collection_date="2011"
                     /collected_by="Merck Study for Monitoring of Antimicrobial
                     Resistance Trends (SMART)"
     gene            join(3681..4073,1..309)
                     /locus_tag="BE949_RS00020"
                     /old_locus_tag="BE949_00020"
     CDS             join(3681..4073,1..309)
                     /locus_tag="BE949_RS00020"
                     /old_locus_tag="BE949_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(567..2714)
                     /gene="mobQ"
                     /locus_tag="BE949_RS00025"
                     /old_locus_tag="BE949_00025"
     CDS             complement(567..2714)
                     /gene="mobQ"
                     /locus_tag="BE949_RS00025"
                     /old_locus_tag="BE949_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            2893..3147
                     /gene="mobC"
                     /locus_tag="BE949_RS00030"
                     /old_locus_tag="BE949_00030"
     CDS             2893..3147
                     /gene="mobC"
                     /locus_tag="BE949_RS00030"
                     /old_locus_tag="BE949_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(2357..2715)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2716..2892
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2..309
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.81e-160"
     Typing          complement(571..2801)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.989"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgggtagtcg tgaagaacgt ctccagcctt tccagcacct acagcatccg catttacgag
       61 ctgctccagc agttccgcag caccggtgac agaacgatag ctatcgatga tttccgaacc
      121 atgcttgccg ttgaagataa atatcaacaa ttcaaagact tgaataagat gataatcaag
      181 ccagccatcg ccgaactgaa cgaaaaaagc gatctggttg taactgttga gacgatcaaa
      241 aaggggcgca cggttgtagc cctgcatttt cgcttcaagg aagacaaaca gatcaagatg
      301 acgatctaaa atcgatttta aggcacctaa aacgctccct gctgcgtttt ttttgtgtta
      361 gtagtgcgaa ggtactattg aagtgaaaaa ttgcgtcaaa aacgctctga tgcagcgaaa
      421 aaagaccccc caatttttgg gggcggaaaa acaaaactat gcttgcgtta tgtgagtgtg
      481 cttcttgcat tcatccctgt cgatgaacat cgaagaacat cgacgaacat aacacgatgt
      541 taaacaaaac aaaatattgt tcacattcac tgtgaccaac cgcgatcatg tctcctctcg
      601 gtggtgtgtg ttcgtgcatc ttcctcgcgc tgccattcct cccaggtacg attaaaaccc
      661 agcgagcgga acagatgatc cggctgtttt tctggtgtgt tgacggcagc aaaaagcggt
      721 gcctcacggg ttttcagatg aaactgcatc agttctggtg caggtgatat gtggctgtct
      781 gcatcgtgat ttacggcaac accataccct gcataccgta accccttatc ggtcagcgca
      841 ctgatgacca gatgcctgtt atcggctgag ggaacggcaa acgcgggata cagcgtacca
      901 ttaagccgga taacaccgcc cagagagtca tccacgccca tgaccagcaa aagctgaagc
      961 tggcgggttt cccggtcgac agaactcatg accggcatca gcgtcggtgg cagacgtccc
     1021 tgcataaaat ccaccttcct tgccgctggt gatggcatgg ctggctggcg gtcttctcca
     1081 agacagaaag actccggcca tgtcttcggc tggcggagat tgcgggtatg ttgcggccat
     1141 gtctggcaaa cctgcgtcag cagttgctca aaggttgcag aggaataaac acgttgcagt
     1201 ggcggacgtt ggccgatttc gcttcggtca cagatataac cactcctgtc acgcattaaa
     1261 tcaacactct caaagattct ggtgcggtat ttctccagct cgttccccca gagcgtatat
     1321 tctccgttcc ggttgtgaag cagaaggtaa caggtcattt ccccgttacc aggcatggga
     1381 gcctcgccat accccgtaac gactgctctc agcacccctg aatccataac ggcattgtca
     1441 tttttcctga gctgctccgc gcgactacgt tcagtatctt cctgttcctt ccggcgcaat
     1501 tcctcttcac gtttaagcaa ctgggctctg agagctgcaa ggccggattc cccccactgc
     1561 tgccactgtt tttcaaatgc catctgactc gcgggaatgt cctgtttctc caccttccgg
     1621 taatgctgtc gtaatttttc catttgctgg ctcagagact ggatttttcc ctgctcacta
     1681 cggtattccg gtgccgccca ttgcatcagg ggaatacggg aaaaaccgga cgaaaacggc
     1741 gatgattttt tcaggctggc agcatggctc atcgcttcct ttcgttcggt gttcagctct
     1801 tcccagcgtc tgcgcgtcgc ctgcatatct gccatcatcg acatggcatt actggtgagt
     1861 ttccatgccg agttactgtc cttcggcaac gcttcgataa tgtcacgtag tgtgtacgaa
     1921 gttgcgttgt caggaagccc gatattcaga aggtcagcca cacggcgggc aggctctgtc
     1981 agccctccgt tcctgagttc ggtgcgggaa atctgtattt ccaggttaag cctgttaatc
     2041 agacgattct gttcgccgcg ttctgtttcg ataccgcgtg tttccatcgc acaggcagac
     2101 tttccgaggt gaataccagg cgttttctca agcccctgtc gctcgtatga acgatggtct
     2161 atctcttcct gataaccggc gttcgccagt gccctgtttg catggtcagc ccatgatgcc
     2221 cgccacgtct cggcatgtgt ccggtcgttc cagtccctga cctttccgct gaacccagca
     2281 ggtccgatag ccctcgtggt cagcatgatg tgcgcatggg gatttgtttt atccatgtga
     2341 tggaacgcca catcagcaat catgccctga ctgacaaagt tttctctgac aaaggcgaga
     2401 acggtttccc gtgcggcatc ctgcggcagt tcgtggggga tggcgagttc aatttcccgc
     2461 gcaagttgtg agttcttccg gcgttcggcc ttctcaacgg cattccagag ttcggcacgg
     2521 ttcgtacacc aggcaggggc ggtggcgggg gtcagaatca cagcctcagc cacaccgctt
     2581 ttgcgggtgt agtcgtgagt cagtccggta cgctcatcaa gcatcagtga accggaacga
     2641 taggcagcgg aagctacggc gctgtagccg ctgcttctgg agataatttt catgctgaga
     2701 tgataaatcg ccatttctgc cgtaatgcct ttttgtagtt ttctttcagc cccgcagggc
     2761 gcacacaatc gaaacggagt ggagattgta taagtgcgca cttcgtgatt tgccgtgcat
     2821 tatgccagct cagtttttaa actaccagtg acgcgctaaa atgcgatttt tcatcgcgca
     2881 cggagttaca tcatgagcag aacgctcgaa cagaagattg ctgatgctga ggcgagatta
     2941 cagcgcctga aggcgaagag ccggagcctg gacacagcgc agaaggttgt tgtgggtgct
     3001 gcattgctgg caaaggtcag aaagccggag gaggtgcagt tgcgtgcctg gttactccag
     3061 ttcctgaagg cagaggtgac acgacaggct gatgtgacgc gcatactgcc gctgattaac
     3121 gagctggagg cgctgccaga acagtgattg tatcgtccgc tcgccgcagc cccagcgacc
     3181 gagcgttagc gagcgagtgg gcgaggaagc ggaagtgaat cagtcgccag gttgagcaca
     3241 tacgcacggt ttttcggcag gtgatatgcg ctgaacccgc atgaatactg ggtttccaca
     3301 gggttggagg gggtgtattc gaaacttgca attctgcttt attgcaacgc attgttttta
     3361 ttggtttttt gttttcagca ccccaagtta tccacaggct cgccgttaag tttttaagat
     3421 cttattttta gatctttttt tttaaaacct ttttaggtct tgtaacacca tgattttaaa
     3481 gatgttctgg caagataagt ggagcttttg ggggtgaaag gtggagcttt tgggggtgaa
     3541 aggtggagct tttgggggtg aaaggtggag cttttggggg tgtaatccac agaaaaaggg
     3601 ggttgcaaaa aaaaaacttc cacccatatg ataagtggcg tacagtaaaa aaacgccacc
     3661 aatagcaacg agcaagcgaa atgtcagatc tgatagctta caaatcaaac gcccttatcg
     3721 aggcaagcta caagctcact ttgcaggaac agcgcttcct gctggtctgt atcagccgcc
     3781 taaaatccgg agaagattcc ccttcgccgg atgaacaaaa aaccatgacc atcacggctg
     3841 cagagtttca tgatgccttt cctgacatgg gacggcagca cgctgaagct aggctcaagg
     3901 aagcgataga tcgcctctgg gacaggtcag taatcatcaa gaacgaggaa aaacgagaag
     3961 agttccgctg ggtgcagtac cgggcgcagt atttcaaagg tgaggcgaaa gtagatatca
     4021 ccttctccga tgccatcatg ccgtatctga cacagctcaa ggggcagttt acg
//
