LOCUS       NZ_CP018971             3164 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_545 plasmid pEC545_5, complete
            sequence.
ACCESSION   NZ_CP018971
VERSION     NZ_CP018971.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511169
            Assembly: GCF_002012045.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002012045.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:48:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,033
            Genes (coding)                    :: 4,867
            CDSs (with protein)               :: 4,867
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 62 of 166
            Pseudo Genes (incomplete)         :: 110 of 166
            Pseudo Genes (internal stop)      :: 30 of 166
            Pseudo Genes (multiple problems)  :: 31 of 166
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018971.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_545"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC545_5"
                     /geo_loc_name="Viet Nam: Ho Chi Minh City"
                     /lat_lon="10.82 N 106.62 E"
                     /collection_date="2011"
                     /collected_by="Merck Study for Monitoring of Antimicrobial
                     Resistance Trends (SMART)"
     gene            883..1764
                     /locus_tag="BE949_RS00005"
                     /old_locus_tag="BE949_00005"
     CDS             883..1764
                     /locus_tag="BE949_RS00005"
                     /old_locus_tag="BE949_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_078207380.1"
                     /translation="MRNTALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPENGHAHLLYALETSIRTAPDGKMK
                     PLRYAAAVENALRKKLDADAGYSGLICKNPNHDHWKIAVWQPELYTLDWLADSLDLSAA
                     NDKEIVADYGLGRNCTLFDRTRKWAYRAIRQGWPEYEQWLQACYERASAYNLQFSVPLD
                     DKEVIGIAKSITKWTHSKLNEMTFSDYVARTHNSAIQSERGKKGGRPSLGEPWLDSGIS
                     RATYFRRKKKCT"
                     /name="replication initiation protein"
     gene            1755..2516
                     /locus_tag="BE949_RS00010"
                     /old_locus_tag="BE949_00010"
     CDS             1755..2516
                     /locus_tag="BE949_RS00010"
                     /old_locus_tag="BE949_00010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_078207381.1"
                     /translation="MYLVKSCKKKDHPHQSGTLKIGSLAEYRDTENKQVEDREEGFYRI
                     KFDLNDKWISTDLFNYLNYSHSSNLSAHIHLLNIKNQDFSSVRVDYKADYKWVNHNRFI
                     FCITKIERVEEAQGIFSGYDDSWSVNYNKIAWLKRSMEMAVLDKIKGMISSGNLIFGDS
                     FNNPDKISIESYSQDIIYQARDLYLGNKDIDPMTDTLISLFKNVKFIKPVYFKKEKELR
                     FVFDFYYDGQIVFPQINSLIIPSEGIVDLFD"
                     /name="hypothetical protein"
     gene            2864..3055
                     /locus_tag="BE949_RS00015"
                     /old_locus_tag="BE949_00015"
     CDS             2864..3055
                     /locus_tag="BE949_RS00015"
                     /old_locus_tag="BE949_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_078207382.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     KRLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
ORIGIN
        1 ttttttattt atttttcatg aagttgcgat gaaaatcgca gctgcgttag gtgtatgtca
       61 ttttttagta tataaaacga ccactcccga gtgctggcgc cgcaggttaa ggatgattct
      121 ggctaaaaag cgtccttttc cgggaaaggg tcatgctcgt atgaccctat gggttcagcg
      181 actacacata aactacaaaa ttgcatctgc ttttacccca gaacgcgggt gtacaacaga
      241 attacatcaa aagtacatca aaattacatc acttttacgt cagatcgcgg gtgtacaaca
      301 gaattacatc aaaagtacat cacttttacg tcacttttac atcattttta cgtcagatct
      361 gacctctgaa ctattcgtac atccgccccc tcttcctggg cctcctgcac gtgctgaaaa
      421 tggtttttcc ggctgtgccg gcaacgcctc acggccacgc aataaattgc gcatccgttc
      481 accgttatcg gggtggttcg tggtggcttt cagggcgttc atcggatttt agccgctaaa
      541 atcccggcca ggtatgtccg tgcatagcca tgcagtcgcg ccagaatcat caggggcgtt
      601 ttttctcgat atgggcacag aaaggtgtgt gtttcaggcc ggaggcctca gaaacgccct
      661 gagggcctct gaggcggtgc gggcaacgga tgttatggta aatatcggcg ggatcgacag
      721 aagtcgtcag acggtgtaac gttaaggcca gaaaaaacaa agccccggaa atcatgtcat
      781 atcttggcgg atcgcatgat aaccagggcc aggtcgaaac ctaagaagga tattagcaca
      841 tgaaccgtgc cagacaacag cccgccataa gcgcagggca cattgcgtaa cactgcactc
      901 gaacttttta acgaacgact cccccacaag ccgtacttct cggacgattt acacttcggt
      961 gtgcgcatcg ccgggaaaga gcgcgcgata ctcgcgaagt acatccagtt caaccagccg
     1021 cacgctatgt tctggctggg cttcgatgtc gatcgtgccg gtgccgccat cgactggagc
     1081 gaccggaacg cgcctgcgcc cacgctgacc atcaccaacc cggaaaacgg gcacgcgcac
     1141 ctgctgtacg cgctagaaac ctcgatacgc accgcacctg acgggaaaat gaagccgctg
     1201 cgctacgccg ccgccgtgga gaacgcgctg cgtaaaaaac tggacgctga cgcggggtat
     1261 tcggggctga tctgcaagaa tccgaaccac gaccactgga aaattgcagt ctggcagccg
     1321 gaactctaca cgctggactg gctggccgac tcgctcgacc tgagcgccgc aaatgacaaa
     1381 gagatcgttg ccgattacgg tttaggtcgt aactgtacgt tgttcgacag aacccgtaag
     1441 tgggcatatc gtgctattcg tcagggctgg cctgagtatg agcagtggct tcaagcatgc
     1501 tatgagcgag ccagcgccta caacctgcaa ttttctgtac cgctggatga taaagaggta
     1561 atagggatag ctaaaagcat tactaaatgg acacactcta agttgaatga aatgacattc
     1621 tcagattatg tcgctagaac tcataactct gccattcaat ccgaaagagg taaaaaaggt
     1681 ggcagaccat cactaggcga accgtggcta gattctggta tatccagagc tacttatttt
     1741 aggcggaaga agaaatgtac ttagtcaaaa gttgcaagaa gaaagatcac cctcatcaat
     1801 ccggaactct aaaaattggt tctcttgctg aatatagaga tacagaaaat aagcaggtag
     1861 aagatagaga agaagggttt tatagaatta agtttgacct gaatgataag tggataagca
     1921 ctgatttgtt taattatttg aactactcgc acagtagcaa cttgagcgct catattcacc
     1981 ttctaaatat taaaaatcaa gattttagta gcgttcgcgt tgattataag gcagattaca
     2041 aatgggttaa ccataatagg tttatctttt gtataacaaa aatagaaaga gtcgaagaag
     2101 cacagggtat atttagtggt tacgatgata gttggtctgt taattacaat aaaatagcat
     2161 ggttaaaaag atcaatggaa atggcggttt tagataaaat caaagggatg atttcgtccg
     2221 gaaatcttat ttttggtgac tcatttaata acccagacaa aatatcaata gaatcatact
     2281 cgcaggatat aatataccag gcaagagatc tttacttggg caataaagac attgacccca
     2341 tgaccgacac tttgatttct ctttttaaaa acgtaaaatt cataaagcct gtgtatttta
     2401 aaaaagagaa agaacttaga tttgtttttg atttttacta cgatggacaa attgtattcc
     2461 cacagatcaa tagcctcatt atcccttcag aagggattgt tgacttgttt gattgatagc
     2521 gaatgagact aaagcctata tcagataaca gccctctgac agcagagggc tttttttgta
     2581 cccagcaccg agttagatat acagcaatac catttaagcc agtacacacg gagtcccctc
     2641 tggcgagggt tccccgtgac tggttcaggg gattacatcc cccgaaaccc ccgtgagtat
     2701 gagtgcgtat ctttgcgaat ttttcgcact taaaacgcga atattctcac ttcattcgca
     2761 cgcaacgctc gccgcagact catgaccgaa cggagtgagc gaatgagcga ggaagcggaa
     2821 aaccatcaga acgtacagta agcactttcg cgccggggcc tttatgaaca aacaacaaaa
     2881 gacagccgtt aacatggcta aattcatcca ggatcagtcc ctgcttcttc tggacaggct
     2941 taatgagctg gatctggatc atgaggctga tttctgcgag aaactccatg aggatgcgaa
     3001 acgcctttac cgaagcttgt cagcgcgcct gaatgagcag caggacggtg cgtaatggct
     3061 ccgttgtggg tattaccgga gtaataccgg catcgcctta attttcattg tcttggcgct
     3121 taatattatt accccggtaa tacccagaat ccctttattt tttc
//
