LOCUS       NZ_CP018949            16559 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_276 plasmid pEC276_KPC, complete
            sequence.
ACCESSION   NZ_CP018949
VERSION     NZ_CP018949.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511184
            Assembly: GCF_002011965.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16559)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002011965.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:29:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,101
            CDSs (total)                      :: 4,988
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 67 of 186
            Pseudo Genes (incomplete)         :: 139 of 186
            Pseudo Genes (internal stop)      :: 37 of 186
            Pseudo Genes (multiple problems)  :: 49 of 186
            Pseudo Genes (short protein)      :: 1 of 186
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018949.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..16559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_276"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC276_KPC"
                     /geo_loc_name="USA: Pittsburgh"
                     /lat_lon="40.44 N 80.00 W"
                     /collection_date="2010"
                     /collected_by="Merck Study for Monitoring of Antimicrobial
                     Resistance Trends (SMART)"
     gene            join(15963..16559,1..2433)
                     /gene="tnpA"
                     /locus_tag="BE964_RS00005"
                     /old_locus_tag="BE964_00005"
     CDS             join(15963..16559,1..2433)
                     /gene="tnpA"
                     /locus_tag="BE964_RS00005"
                     /old_locus_tag="BE964_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152392.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn4401 family transposase"
                     /protein_id="WP_004152392.1"
                     /translation="MSKKNKLLSVLSDAEQEALYGLPEFDDAQQLEYLAVTETELALAN
                     SRPSLHAKVYCLLQIGYFKAKHAFFSFDWDEVEDDCAFVLSRYFHGEVFEPKAITKHER
                     YTQREQIAKLFGYWPWSAAFLPQLEQQAARTVRRDVTPGFVAAELIVWLNEHKIIRPGY
                     TTLQELVSETLSAERRRLGNLLEQALDESAKAALSQLLVRDDTLSQLAALKQDAKNFGW
                     RQMVREREKRATLEPLHAIAKTLLPKLDVSQQNLLYYASLANFYTVHDLRNLKADQTQL
                     YLLCYAWVRYRQLSDNLVDAMAYHMKQLEDESSAGAKQSFADEQVRRQQETPRVGRLLS
                     LYVDDSVADPTPFGEVRQRAYKIMPRDTLQTTAQRMSVKPASKLALQWQAVDGLADRMR
                     RHLRPLYVALDLSGSNPDSPWLAALAWAKGVFAKQQRLSQRPLDEWPAATLPKRLRPYL
                     LTFDADGKPTGLHADRYEFWLYRQIRKRLQSGELYLDDSLQHRHFSDELVEMDRKIDVL
                     AKMEIPFLQQPVHAQLDALTAELRTQWLAFNRELKQGKLTHLEYNKDTQKLTWRKPKSE
                     NEKAREKAFYEKLPFCDVADVFRFVNDECKFLSAFTPMQPRYAKKVADADSLMAVIIAQ
                     AMNHGNHVMARTSDIPYHVLDSGYQQYLRQATLHAANDCISNAIATLPIFPYYSFDLET
                     LYGAVDGQKFSVERPTVKARYSRKYFGRGKGVVAYTLLCNHIPLNGYLIGAHDYEAHHV
                     FDIWYRNTSDIMPTAITGDMHSVNKANFAILHWFGRRFEPRFTDLNAQLKELYCADDPA
                     QYQACLIRPVGKIDCDLIHREKPNIDRIVATLGLKEMTQGTLIRKLCTYTTTNPTRRAI
                     FEFDKLIRSIYTLRYLRDPQLERNVHRSQNRLESYHQLRSAIAQVGGKKELTGRTDIEI
                     EISNQCARLIANTIIFYNSAILSRLVTKYEAAGNSKALALITKISPAAWRHILLNGHYT
                     FQSSGKTIDLDAIVAGLELE"
                     /name="tnpA"
     gene            2540..3565
                     /gene="istA"
                     /locus_tag="BE964_RS00010"
                     /old_locus_tag="BE964_00010"
     CDS             2540..3565
                     /gene="istA"
                     /locus_tag="BE964_RS00010"
                     /old_locus_tag="BE964_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490293.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="WP_004199214.1"
                     /translation="MLTQEQTVEIRVLARQGHSIRHIARTLGVSRNTVRRYLRDPSVAR
                     YQPREPRPTKLGPFESYLRQRVEQAHPIWLPATVLNREIRAQGYGGGLSLLRAFLATLK
                     PARREAGPAVRFETEPGRQLQADFVVLRRARSPMSAFVATLGYSRMTFVTFVPDESFES
                     VRDSLLLAFDYLGGVPREVLFDNMKTVVLERDAYGDGKHRFHPGLLQLADDLGFRIRLC
                     RPYRAQTKGKVERFNRYFRESFYNPLLTRMKGTGLLLDCAAANRRVRDWLADEANVRVH
                     ATLNERPIDRWRQEREHLQPLPSRVRRDEAPLLDNSLRPVPLESLQHPLSVYDAIGEAC
                     R"
                     /name="istA"
     gene            3562..4341
                     /gene="istB"
                     /locus_tag="BE964_RS00015"
                     /old_locus_tag="BE964_00015"
     CDS             3562..4341
                     /gene="istB"
                     /locus_tag="BE964_RS00015"
                     /old_locus_tag="BE964_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISKpn7 family helper ATPase
                     IstB"
                     /protein_id="WP_004152394.1"
                     /translation="MNLQHERIDALCQQLKLERVATHYPVLAQQAVREDFSFTDFLEHL
                     LRHEAGCRQQRSRELLTRMACFPGIKTLEDYDFSFNPGVPKALVQELGSLAFVERAENV
                     VLIGPSGIGKTHLAIALGYKAAQAGVKTRFITAADLMLQLSTAQRQGRLKEYFNRAILG
                     PRLLVIDEIGYLPFGREEANLFFQVVAKRYEKGAMILTSNLPFSQWADAFAGDTTLTAA
                     MLDRLLHHAHILTLSGESYRLKDKRKAGVVRKNSKPE"
                     /name="istB"
     gene            4338..4577
                     /locus_tag="BE964_RS28110"
                     /old_locus_tag="BE964_00020"
     CDS             4338..4577
                     /locus_tag="BE964_RS28110"
                     /old_locus_tag="BE964_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004152395.1"
                     /translation="MIQVGQYYFGDSGVKWVSFQLVLTPAYPRCYLRAPIVGAWPGLPE
                     AVRNVDAEVRRGGRPMNADLIRFSSSVIIPAVAA"
                     /name="hypothetical protein"
     gene            4629..5510
                     /locus_tag="BE964_RS00025"
                     /old_locus_tag="BE964_00025"
     CDS             4629..5510
                     /locus_tag="BE964_RS00025"
                     /old_locus_tag="BE964_00025"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /protein_id="WP_004199234.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSIG
                     VYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALVPW
                     SPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFRLDR
                     WELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNHRIRA
                     AVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKHSEAVIAAAA
                     RLALEGLGVNGQ"
                     /name="carbapenem-hydrolyzing class A beta-lactamase KPC-2"
     gene            complement(5760..7079)
                     /locus_tag="BE964_RS00030"
                     /old_locus_tag="BE964_00030"
     CDS             complement(5760..7079)
                     /locus_tag="BE964_RS00030"
                     /old_locus_tag="BE964_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
                     /protein_id="WP_004152397.1"
                     /translation="MAASYLPYRPDQSYLLPPSLGEWLPEGHLAYFISETVDTLDLSAF
                     HARYAGGGPGNQPFHPAMMVKVLIYGYASGVFSSRKLARKLYEDVALRVLAAGNFPAHR
                     TLSDFRALHLTELENLFVQVVQLARECGLVKLGTIAVDGTKVKANASRHKAMSYKRMKP
                     AEDELHCEIKALLDRAKATDDQERNEPELDIPAEISRREKRLEAIQAAKARLEARQREA
                     DQARGRSEDDGRRPRHPDGSDKGGGSYKREFGVPDDRDQESFTDPDSRIMKHAGGGSEQ
                     SYNGYTAVDAEHQIIVAAELTNCAADSQALLGMLAAVQANTGEMPAQTLADAGFRSEAV
                     LAKVADHHGDVIVALGREGREDAKVNAKTHPHTAAIAAKLKTEQGDAAYRRRKSIVEAP
                     NGWIKAVMGLRQFSMRGLDKVQAEWKLVCMALNLRRMAYL"
                     /name="IS1182-like element ISKpn6 family transposase"
     gene            complement(<7438..7893)
                     /locus_tag="BE964_RS28115"
                     /pseudo=""
     CDS             complement(<7438..7893)
                     /locus_tag="BE964_RS28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /name="MbeB family mobilization protein"
     gene            complement(<7931..8629)
                     /locus_tag="BE964_RS28120"
                     /pseudo=""
     CDS             complement(<7931..8629)
                     /locus_tag="BE964_RS28120"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(8571..8894)
                     /locus_tag="BE964_RS00040"
                     /old_locus_tag="BE964_00040"
     CDS             complement(8571..8894)
                     /locus_tag="BE964_RS00040"
                     /old_locus_tag="BE964_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            8958..9149
                     /locus_tag="BE964_RS00045"
                     /old_locus_tag="BE964_00045"
     CDS             8958..9149
                     /locus_tag="BE964_RS00045"
                     /old_locus_tag="BE964_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            9819..9977
                     /locus_tag="BE964_RS28125"
     CDS             9819..9977
                     /locus_tag="BE964_RS28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(10176..10313)
                     /locus_tag="BE964_RS00060"
                     /old_locus_tag="BE964_00060"
     CDS             complement(10176..10313)
                     /locus_tag="BE964_RS00060"
                     /old_locus_tag="BE964_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            10475..10702
                     /locus_tag="BE964_RS00065"
                     /old_locus_tag="BE964_00065"
     CDS             10475..10702
                     /locus_tag="BE964_RS00065"
                     /old_locus_tag="BE964_00065"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(10699..12267)
                     /gene="cea"
                     /locus_tag="BE964_RS00070"
                     /old_locus_tag="BE964_00070"
     CDS             complement(10699..12267)
                     /gene="cea"
                     /locus_tag="BE964_RS00070"
                     /old_locus_tag="BE964_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            12527..12964
                     /locus_tag="BE964_RS00075"
                     /old_locus_tag="BE964_00075"
     CDS             12527..12964
                     /locus_tag="BE964_RS00075"
                     /old_locus_tag="BE964_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021532742.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSKGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            13045..13464
                     /locus_tag="BE964_RS00080"
                     /old_locus_tag="BE964_00080"
     CDS             13045..13464
                     /locus_tag="BE964_RS00080"
                     /old_locus_tag="BE964_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(13782..14015)
                     /locus_tag="BE964_RS00095"
                     /old_locus_tag="BE964_00095"
     CDS             complement(13782..14015)
                     /locus_tag="BE964_RS00095"
                     /old_locus_tag="BE964_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(14138..15853)
                     /gene="tnpR"
                     /locus_tag="BE964_RS00100"
                     /old_locus_tag="BE964_00100"
     CDS             complement(14138..15853)
                     /gene="tnpR"
                     /locus_tag="BE964_RS00100"
                     /old_locus_tag="BE964_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn4401 family resolvase TnpR"
                     /protein_id="WP_004152391.1"
                     /translation="MKTGQTTASSSTPVGFPDADSLAALRGWYAGLSSRAAVEHYLAAR
                     KAAGQSARGMLGHIRRQLIVFANSRHRPDLADVFQHAPEERQGRAGAVERAIRLLPTLP
                     APKPQISDSIDQWLSPRTVRALQAYGIHTLSALTVRIPRRRGWWKAIEGLGMTGARQVE
                     AFFAAHPQLTERARALIPIIQPSGIVPWEQLRLPNEVDGSQGNFRAPRNACTLNATNDY
                     EAVQAWLALHETVATQRAYRKEAARLILWAIVERGRALSSLTTDDAIAYRAFVRHPTPR
                     ERWVGPPRPRDSVEWRPFSGGLSARSAAYALTVLSALFRWLIEQRYVLANPFAGVKVRG
                     HALRPALDTARGFTEGEWLLLRAIADGLEWSYGWSEPAAQRLRFMLDFGYATGLRASEL
                     VGAVLGNVRLDEHGDHWLHLVGKGGKPGKVALPPLAYTALDQYLAQRQLPVSRERWNPK
                     TPLVGSLGEDGNAGITGPHLWCVLRRFFTQAAEAIEADHPVAAEKLRRATPHWMRHSHA
                     SHALARGAQLTAVRDNLRHASIATTSMYLHGDELERARQMRQAFGARHVKRGVD"
                     /name="tnpR"
     ARG             4630..5507
                     /name="blaKPC-2"
                     /gene="blaKPC-2"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(9235..9322)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          9333..9891
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.544"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7434..8581)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.739"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(13679..14085)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.105"
                     /coverage="100.0"
                     /evalue="1.94e-51"
ORIGIN
        1 cttctggtcc gtgatgacac cctgtcgcaa ctggcggcgc tcaagcagga cgccaagaat
       61 ttcggctggc gccagatggt ccgcgagcgc gaaaagcgcg ccacgctgga gccgctgcac
      121 gcaatcgcca agacactgct gcctaaactc gacgtctcgc agcagaacct gctgtactac
      181 gcgagcctgg caaacttcta caccgtccac gacctgcgca atctgaaggc cgatcagacc
      241 caactctacc tgctgtgcta tgcctgggta cgctaccggc agctctccga caacctggtc
      301 gatgcgatgg cgtaccacat gaagcagttg gaggacgaaa gcagcgctgg tgccaaacaa
      361 tcctttgccg acgagcaggt gcgccgtcag caggaaacgc cgcgggtagg ccgcctgctg
      421 tcactctacg ttgatgatag cgtggccgat cccacgccct tcggcgaggt gcggcagcgc
      481 gcctacaaga tcatgcctcg cgatacgctg caaaccacgg cgcagcgtat gagcgtgaag
      541 ccggcgagca agctggcctt gcagtggcag gcggtggacg ggctggccga ccgcatgcgc
      601 cgccatcttc gtccactgta cgttgcgctc gacctctccg gctccaatcc agacagtcca
      661 tggcttgccg cgctggcctg ggccaaaggt gtttttgcca agcagcagcg gctgtcgcaa
      721 cggccactcg acgagtggcc cgcggcgaca cttccgaaac gcttgcggcc gtacctgctg
      781 accttcgacg ccgacggtaa accaaccggc ctgcacgctg accgctacga gttctggctg
      841 taccgccaga tcaggaagcg tctccaatcg ggtgaactct acctcgacga cagcctgcaa
      901 caccggcact tctcagatga actggttgag atggacagga agatcgatgt gctcgcaaaa
      961 atggagatcc ccttcctgca gcaacccgtc catgcccaac tcgatgcgct gactgccgaa
     1021 ctgcgcacgc agtggctggc gttcaaccgc gagctgaaac agggcaagct gacgcacctc
     1081 gaatacaaca aggacacgca gaaactgacc tggcgcaagc ccaagagcga gaatgagaaa
     1141 gcgcgcgaaa aggcattcta cgagaagctt ccgttctgcg acgtggccga cgtgtttcgc
     1201 ttcgtcaacg acgagtgcaa gtttctgtcg gcgttcacgc ccatgcagcc gcgctacgcg
     1261 aagaaggtcg ccgacgctga cagcctgatg gcggtcatca tcgcgcaagc gatgaaccac
     1321 ggcaaccacg tcatggcgcg caccagcgac atcccgtacc acgtgctgga tagcggctac
     1381 cagcagtacc tgcgccaggc aacgctgcac gcggccaacg actgcatcag caacgccatc
     1441 gccacgctac cgatcttccc atactactcg ttcgacctcg aaacgctgta cggtgccgtc
     1501 gacgggcaga aattcagcgt cgaacggccg accgtgaagg cgcgctattc gcgcaagtac
     1561 ttcgggcgcg gcaagggcgt ggtcgcctac acgctgctgt gcaaccacat cccgctcaac
     1621 ggctacctga tcggcgcgca cgactacgag gcccaccacg tgttcgacat ctggtatcgc
     1681 aacacgtcgg acatcatgcc gaccgcgatc accggcgaca tgcacagcgt caacaaggcc
     1741 aacttcgcca tcctgcattg gtttggccgg cggttcgagc cgcgcttcac cgacctcaac
     1801 gcgcaattga aagaactcta ctgcgctgac gatccagcac agtatcaggc gtgcctgatt
     1861 cggccagtcg ggaaaatcga ctgcgatctc atccatcgcg agaagccgaa catcgaccgg
     1921 atcgtcgcca cactcgggct gaaggaaatg acgcagggca cgctgatccg caagctgtgc
     1981 acctacacca cgacgaaccc gacgcggcgc gcgatcttcg agttcgacaa gctcatccgc
     2041 agcatctaca cgcttcgcta cctgcgcgat ccgcaactgg aacgcaacgt gcaccgctcg
     2101 caaaatcgcc tcgagtccta ccatcagcta cgctcggcca ttgcccaggt cggcggtaag
     2161 aaggaactga ccggtcgcac cgacattgaa atcgagatca gcaaccagtg cgcacggctg
     2221 atcgccaaca cgatcatttt ctacaactcg gcaatcttgt cgcggctggt gaccaagtac
     2281 gaggcggccg gcaacagcaa ggcgctggca ctcatcacaa aaatttcgcc tgcggcatgg
     2341 cggcacatcc tgctcaacgg gcactacacc tttcaaagca gcggcaagac gatcgatctc
     2401 gatgcgatcg tcgcaggcct ggaactggag tgacggaatt ttcagcggtt ccgtgttagc
     2461 agcagtgtaa aactgaccca ccccagcgaa gtaaaactga tccacctggg ctagtttggc
     2521 ggccttttcg aggccgtcga tgttgaccca ggagcaaacc gtggaaatca gagttcttgc
     2581 ccgacagggc cactccatcc ggcacatcgc ccgcacgttg ggggtctcgc gcaataccgt
     2641 gcgccgctac ctccgcgatc cgtcggtagc gcgataccag ccccgtgaac cacgccccac
     2701 caaactgggg ccatttgaat cttatctgcg gcaacgggtc gagcaggccc accccatctg
     2761 gctgcctgcc accgtcctga atcgggaaat ccgcgctcaa gggtatggcg gcggtctctc
     2821 gttattgcgc gccttcctcg ccacgctcaa gcccgcccgc cgcgaagcag gtccggccgt
     2881 ccgtttcgag accgagcccg gacggcagtt gcaggctgat ttcgtggtct tgcgccgggc
     2941 cagatccccc atgtcggcct tcgtcgccac gctgggatac agccgcatga cgtttgtcac
     3001 cttcgtaccg gacgaatcct tcgagtccgt gcgggactcc ctgttgctgg cttttgacta
     3061 tctgggcggt gtgccccgcg aggtgttgtt cgacaacatg aagaccgtgg tgctggaacg
     3121 cgatgcctac ggtgacggca agcaccgttt tcaccccggc ttgcttcaac tggccgatga
     3181 cctggggttc cggatccggc tgtgtcgccc gtatcgggcc cagaccaaag gcaaggtcga
     3241 gcgcttcaat cgctacttcc gcgagagctt ctacaacccg ttgctgaccc ggatgaaggg
     3301 cacggggctg ctgctcgatt gtgctgctgc caaccgacgc gtccgtgact ggttggcaga
     3361 cgaggcgaat gtgcgggtcc acgccaccct gaacgagcgc cccatcgacc gttggcggca
     3421 ggagcgggaa cacctgcaac ccttgccatc ccgtgtgcgg cgcgacgagg cccccctgct
     3481 ggataacagt ctccgcccgg taccactgga atctctccag cacccactgt ctgtctatga
     3541 cgcaattggg gaggcctgcc gatgaacctc cagcatgagc ggatcgacgc cctgtgccag
     3601 caactcaaac tcgaacgggt ggcaacgcat taccccgtcc tggcgcagca ggcggtgcgc
     3661 gaggacttca gcttcaccga cttcctggaa cacctgttgc ggcatgaagc ggggtgtcgc
     3721 cagcagcgca gccgcgaact gctgacgcgg atggcttgct tcccgggcat caagaccctg
     3781 gaggactatg acttcagctt caaccccggc gtgcccaagg cactggtgca ggagctgggc
     3841 agtctggcgt ttgtcgaacg ggccgagaac gtggtgctga tcggaccctc tggcatcggc
     3901 aagacccacc tcgccatcgc cttgggttac aaggccgcgc aagccggggt caagacacgc
     3961 ttcatcacgg cagccgacct gatgctgcaa ctgagcacgg cgcagcgaca agggcggctg
     4021 aaggaatact tcaaccgcgc catcctcggt ccacggctgc tggtgatcga tgagatcggc
     4081 tacctgcctt tcggacgcga ggaagcgaac ctgttctttc aggtggtggc gaagcgctat
     4141 gagaaagggg cgatgatcct gacctcgaac ctgccgttca gccagtgggc cgacgcgttt
     4201 gccggcgata ccaccctgac agccgcgatg ctggatcggc tgctgcacca tgcccatatc
     4261 ctgaccctga gcggcgaaag ctaccgcttg aaggacaaga ggaaggcggg agtggtcagg
     4321 aaaaattcca aacccgaatg atccaggtgg gtcagtatta ctttggtgat tcaggggtaa
     4381 agtgggtcag ttttcagttg gtgttgacac cggcgtaccc tcggtgctat cttcgcgccc
     4441 caatagtcgg ggcttggcca ggacttcctg aggccgtccg taacgtggat gccgaggtca
     4501 ggcgaggtgg ccgacccatg aacgccgacc tgattcgttt ttcaagctcg gtgataatcc
     4561 cagctgtagc ggcctgatta catccggccg ctacacctag ctccaccttc aaacaaggaa
     4621 tatcgttgat gtcactgtat cgccgtctag ttctgctgtc ttgtctctca tggccgctgg
     4681 ctggcttttc tgccaccgcg ctgaccaacc tcgtcgcgga accattcgct aaactcgaac
     4741 aggactttgg cggctccatc ggtgtgtacg cgatggatac cggctcaggc gcaactgtaa
     4801 gttaccgcgc tgaggagcgc ttcccactgt gcagctcatt caagggcttt cttgctgccg
     4861 ctgtgctggc tcgcagccag cagcaggccg gcttgctgga cacacccatc cgttacggca
     4921 aaaatgcgct ggttccgtgg tcacccatct cggaaaaata tctgacaaca ggcatgacgg
     4981 tggcggagct gtccgcggcc gccgtgcaat acagtgataa cgccgccgcc aatttgttgc
     5041 tgaaggagtt gggcggcccg gccgggctga cggccttcat gcgctctatc ggcgatacca
     5101 cgttccgtct ggaccgctgg gagctggagc tgaactccgc catcccaggc gatgcgcgcg
     5161 atacctcatc gccgcgcgcc gtgacggaaa gcttacaaaa actgacactg ggctctgcac
     5221 tggctgcgcc gcagcggcag cagtttgttg attggctaaa gggaaacacg accggcaacc
     5281 accgcatccg cgcggcggtg ccggcagact gggcagtcgg agacaaaacc ggaacctgcg
     5341 gagtgtatgg cacggcaaat gactatgccg tcgtctggcc cactgggcgc gcacctattg
     5401 tgttggccgt ctacacccgg gcgcctaaca aggatgacaa gcacagcgag gccgtcatcg
     5461 ccgctgcggc tagactcgcg ctcgagggat tgggcgtcaa cgggcagtaa ggctctgaaa
     5521 atcatctatt ggcccaccac cgccgccctt gcgggcggca tggattacca accactgtca
     5581 catttaggct aggagtctgc gcggcagagc cgtgtgaccg gttttctgta gagcactgac
     5641 gatggcggcg gcgctctctg caattggcaa ggcgtcggcg ccaaggatac caatcttgcg
     5701 gcgcgcggcg tgttatgacg actggggtgc atttgagccg ccccatttaa ccttcgccct
     5761 cacagatacg ccattcgcct cagatttagc gccatgcaga cgagcttcca ctcggcttgc
     5821 accttgtcca ggcccctcat gctgaactga cgcaatccca tcaccgcctt gatccaacca
     5881 ttcggagcct ccacgatcga cttgcgccgg cggtaagctg catcgccttg ctccgttttc
     5941 aatttcgccg caatcgccgc cgtatgcgga tgggtcttgg cattgacctt ggcatcttca
     6001 cgtccctcgc ggccgagggc aacgatgaca tcgccgtggt gatcggcgac ctttgccaga
     6061 acagcctcac tacggaatcc cgcatccgcc agcgtctggg ccggcatttc tccggtgttg
     6121 gcctgaactg ctgccagcat gcccagcagc gcctgactgt ccgcggcgca gttggtcaac
     6181 tccgccgcca cgatgatctg gtgctcggca tcgaccgctg tgtacccgtt gtagctctgc
     6241 tcggagccac caccggcgtg tttcatgatc cggctgtccg gatcggtgaa gctttcctga
     6301 tcacggtcat ccggcacacc aaactcgcgt ttgtacgagc caccgccctt gtccgagcca
     6361 tccggatggc gaggccggcg gccatcgtct tcgctgcgcc cccgggcctg gtccgcttca
     6421 cgctggcgcg cttccaggcg cgcctttgcc gcctggatcg cctccaggcg cttctcgcgg
     6481 cgagaaatct cggcaggaat gtccagctcc ggctcgttac gctcctggtc gtcggtagcc
     6541 ttggcgcgat caagcagcgc cttgatctcg caatgcaatt cgtcctcggc cggcttcatg
     6601 cgcttatagc tcatcgcctt gtggcggctg gcgttggctt ttaccttggt gccgtcgacc
     6661 gcgatcgtgc caagcttcac cagcccacat tcgcgcgcca gttgcaccac ctgaacgaac
     6721 aaattctcaa gctcggtcag gtgtagggca cggaagtcac tcagcgtgcg gtgggccggg
     6781 aagtttccag cggccagcac acgcaacgcg acatcctcgt acagcttcct ggctagtttg
     6841 cgcgaagaga agacgccgct cgcgtaacca tagatcagca ctttgaccat catcgccgga
     6901 tgaaacggct gattgcccgg accaccgccg gcataccggg cgtggaatgc gctcaagtcc
     6961 agcgtatcga cagtctcgct gatgaagtag gcaagatgcc cttcaggtag ccactctccc
     7021 agagaagggg gcagcagata ggattggtcg ggtcggtaag gaaggtaact ggcagccatc
     7081 cccgtatcgt cctcgatctt ccgccgattt ggcttctgcc gcgcaggctc ctaggccacc
     7141 gtagctaaac atacgagaaa aaccgcttct tgagcgctct ctgagaccac cttgccgaca
     7201 acgcagaaac ggttgcggaa aaatcggcgt ttccggcgta cgccacccga cctggacgaa
     7261 ctattgcgga cctggaactg gagtagcgga attccccggg gtttcagcgt cgcgccacag
     7321 cgcctcagat agatgcggta gcctttggcg ggtcgtcatg ccgcggacca cccccccaaa
     7381 aaattccaca atgcggaatg gcggaatttt ctggggttcc cgcttacccc ccccgtacgg
     7441 aaggacaagg aatttcctgt tcccgtcctg cacaaactcc acgccccatg tcttcgcgtt
     7501 cagtttctgc aatgtctctt cctgcttcct gatttcttcc aggttcgcct gtatcctccc
     7561 tccaagatac cagagcgtcc cgccactcgc ggtaaacagg agaaagacta tccccagtaa
     7621 catcatgccc gtattccctg ccagctttaa cacgtccctc ctgtgctgca tcatcgcctc
     7681 tttcacccct tcccggtgtt tttccagcga ttcctctgtc gaggctgtga acagggctat
     7741 agcgtctctg attttcgtct cgtttgatgt cacagcctcg cttacagatt cgccgagcct
     7801 cctgaactcg ttgttcagca ttttctctgt agattcggct ctctctttca gctttttctc
     7861 gaactccgcg cccgtctgca aaagattgct cataaaatgc tcctttcagc ctgatattct
     7921 tcccgccgtt cggatctgca atgctgatac tgcttcgcgt caccctgacc acttccagcc
     7981 ccgcctcagt gagcgcctga atcacatcct gacggccttt tatctctccg gcatggtaaa
     8041 gtgcatctat acctcgcgtg acgccctcag caagcgcctg tttcgtttca ggcaggttat
     8101 cagggagtgt cagcgtcctg cggttctccg gggcgttcgg gtcatgcagc ccgtaatggt
     8161 gattcaccag cgtctgccag gcatcaattc taggcctgtc tgcgcggtcg tagtacggct
     8221 ggaggcgttt tccggtctgt agctccatgt tcggaatgac aaaattcagc tcaagccgtc
     8281 ccttgtcctg gtgctccacc cacaggatgc tgtactgatt tttttcgaga ccgggcatca
     8341 gtacacgctc aaagctcgcc atcacttttt cacgtcctcc cggcggcagc tccttctccg
     8401 cgaacgacag aacaccggac gtgtatttct tcgcaaatgg cgtggcatcg atgagttccc
     8461 ggacttcttc cggattaccc tgaagcaccg ttgcgccttc gcggttacgc tccctcccca
     8521 gcaggtaatc aaccggacca ctgccaccac cttttcccct ggcatgaaat ttaactatca
     8581 tcccgcgccc cctgttccct gacagccaga cgcagccggc gcagctcatc cccgatggcc
     8641 atcagtgcgg ccaccacctg aacccggtca ccggaagacc actgcccgct gttcacctta
     8701 cgggctgtct gattcaggtt atttccgatg gcggccagct gacgcagtaa cggcggtgcc
     8761 agtgtcggca gttttccgga acgggcaacc ggctccccca ggcagacccg ccgcatccat
     8821 accgccagtt gtttaccctc acagcgttca agtaaccggg catgttcatc atcagtaacc
     8881 cgtattgtga gcatcctctc gcgtttcatc ggtatcatta ccccatgaac agaaatcccc
     8941 cttacacgga ggcatcagtg actaaacagg aaaaaaccgc ccttaacatg gcccgcttta
     9001 tcagaagcca gacattaacg ctgctggaga agctcaacga actggacgca gatgaacagg
     9061 ccgatatttg tgaatcgctt cacgaccacg ccgatgagct ttaccgcagc tgcctcgcac
     9121 gtttcgggga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     9181 gtctgtgagc ggatgccggg agctgacaag cccgtcaggg cgcgtcagca ggttttagcg
     9241 ggtgtcgggg cgcagccctg acccagtcac gtagcgatag cggagtgtat actggcttaa
     9301 ccatgcggca tcagtgcgga ttgtatgaaa agtacgccat gccgggtgtg aaatgccgca
     9361 cagatgcgta aggagaaaat gcacgtccag gcgcttttcc gcttcctcgc tcactgactc
     9421 gctacgctcg gtcgttcgac tgcggcgagc ggtactgact cacacaaaaa cggtaacaca
     9481 gttatccaca gaatcagggg ataaggccgg aaagaacatg tgagcaaaag accaggaaca
     9541 ggaagaaggc cacgtagcag gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9601 aaaatagacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9661 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9721 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgttggtatc
     9781 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9841 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta aggcacgcct
     9901 taacgccact ggcagcagcc actggtaacc ggattagcag agcgatgatg gcacaaacgg
     9961 tgctacagag ttcttgaagt agtggcccga ctacggctac actagaagga cagtatttgg
    10021 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    10081 caaacaaacc accgttggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    10141 aaaaaaagga tctcaagaag atcctttgat cttttctact gaaccgcgat ccccgtcagt
    10201 ttagaagagg aggatggtgc gatggtccct ccctgaacat cacgtatata gttagcctga
    10261 catccaacaa ggaggtttat cgcgaatatt cccacaaaaa atcttttcct cataactcga
    10321 tccttataaa atgaaaagaa tatatggcga ggtttaattt atgagcttaa gatactacat
    10381 aaaaaatatt ttatttggcc tgtactgcac acttatatat atatacctta taacaaaaaa
    10441 cagcgaaggg tattatttcc ttgtgtcaga taagatgcta tatgcaatag tgataagcac
    10501 tattctatgt ccatattcaa aatatgctat tgaatacata gcttttaact tcataaagaa
    10561 agattttttc gaaagaagaa aaaacctaaa taacgccccc gtagcaaaat taaacctatt
    10621 tatgctatat aatctacttt gtttggtcct agcaatccca tttggattgc taggactttt
    10681 tatatcaata aagaataatt aaatccctaa cacctcattt atagtattaa gtttattctt
    10741 atcaatatag gagcatagaa ttcctgtaac aatagcaata ccccaaatac ctaatgtagt
    10801 tccagcaagc aagctaaaaa gtaaagcaac aacataactc acccctgcat ctgcagcttt
    10861 cttctctaat gtaagaaata gtggcttcca gtcacctgta tccttaattt ttaggatatc
    10921 agataccaca tcatatccaa aagaaacatg ccccgtaatc ttcaagtact tggcaaactg
    10981 atctaaatgt ttagcccagt catcatactt caccgatgcc aacgcattaa aaatagcatc
    11041 acgatcggct ttgctgaatt tcttatttaa aacatccttg tatttttcaa aagcagcgag
    11101 agcttcattc acattgccga ttttcttacc tttagactta tcagcaagtt cctgtgccat
    11161 tttcgaatat ttttcaccat atttttcagt cagcgtttga taaaagctaa ctgttgcatc
    11221 aacagcatcc ttaatctgtg aattaaggag attattctgt gcttttttca aattttcttc
    11281 agcttcatga acacgagcga taccggcatt acgattatta ctgacctgag aaatagcctt
    11341 ctggatctga gttatatcag catttatccg gttaatacgt gtttctgatg ctgttacctg
    11401 tttttgtttt tcttctctaa tcttaccggc cccaacccgt cgtctggttg cttcaaaaaa
    11461 aggacggttc tgaagcggat cattggctct tggtgatagt tttttgacca gctcatccag
    11521 ttctttatat ttagcggatg cctgagccag ttcatttcgt tttccagcga gcgttttcat
    11581 ttctgcatca cgggcatgga tactggagct taaacgagaa ttgagagtct taatctctcc
    11641 atccattttc accacttcag attgtgcagc agaaagtttt ttttgggcga tctcaacagc
    11701 tttagcttct tcactcaatg cagccagtcg tttctcttca gcttcagcca gtttcaactg
    11761 gcgttctgtt tcagccttct cccgttcaat ctctttacgt cgttgttctg cttcctgaaa
    11821 agccttttct gctgcttccg cttctttacg ggctttttct tctgctttcg caaggcgcaa
    11881 acgctctgct tccgcctgca tagctgcatt attagcatga gcaagctctg ttgctgaagg
    11941 cgtacgtgag gcattgtgac gaagagcctc attcacgata tccttcaggc gctgagtcag
    12001 cgcatccctg tttgcctttg ctttcgcctg tgcttccgct gcagcttttg cccgggcagc
    12061 ctgctctgcc tgtgttttct ttaattgagc agtagaccat ttagcagttg catgaatagc
    12121 tgcagaactt tcacttttac tgcctccttt tccacctccg ccgccagagc cactcccgtc
    12181 aggagtacca ttcaaaagag taataattac ctgtccctta tcatcataag gaacaccatc
    12241 tttatagtac gctaccgcgg tttccattat aaaatcctct ttgactttta aaacaataag
    12301 ttaaaaataa atactgtaca tataaccact ggttttatat acagcataaa agctacgccg
    12361 ctgcattttc cctgtcaaga ctgtggactt ccatttttgt gaaaacgatc aaaaaaacag
    12421 tctttcacac cacgcgctat tctcgcccga tgccacaaaa accagcacaa acattaccgt
    12481 tctcagacct cattatgttt tactgaaact atgagatgag acatctatgg gacactgtca
    12541 ctttatggca tggcacacac tccgggacgc actaaaaatg acaggcagat cgcgttcaca
    12601 gttttaccgt gatatgcgcg caggccttgt cagttaccgt accggcaggg acggacgacg
    12661 ggagtttgaa accagtgaac tgatccgggc atacggcgaa ttaaagcaga atgagacacc
    12721 agaaaggcac agtaagggac atgcagaaaa tccacatgat cagcagacag aacgcattct
    12781 ccgggagctg aatgagctga aacaatgcct gacgctgatg cttgaggata aacaggcaca
    12841 ggatatggat cgcagacgcc aggaagcaga acgggaacag ctacaaaatg agatagccca
    12901 gctcaggcag gcactggaac tggaaaagaa acggggattc tggtccaggt tgttcggtcg
    12961 ctgaacgctg tcagagactg atgataaaat agtcttcgga taataactca ccgagaataa
    13021 atactttaag gtagggagac actcatgaga cgtaccggaa acaaactttg tcttatcgcc
    13081 atgataacag caacagtagc tctcacagcc tgtaccccaa agggcagcgt ggaacaacat
    13141 acccggcatt acgtatatgc ttctgatgac ggttttgatc ccaacttttc cacccaaaaa
    13201 gccgacacaa cacgaatgat ggtgcctttt tttcggcagt tctgggatat gggagctaaa
    13261 gataaagcga caggaaaatc acggagtgat gtgcaacaac gcattcagca gtttcacagc
    13321 caggaatttt taaactcact ccggggcaca actcaatttg cgggtactga ttaccgcagc
    13381 aaagacctta ccccgaaaaa atccaggctg ctggctgaca cgatttctgc ggtttatctc
    13441 gatggctacg agggcagaca gtaagtggat ttaccataat cccttaattg tacgcaccgc
    13501 taaaacgcgt tcagcgcgat cacggcagca gacaggtaaa aatggcaaca aaccacccga
    13561 aaaactgccg cgatcgcgcc tgataaattt taaccgtatg aatacctatg caaccagagg
    13621 gtacaggcca cattaccccc acttaatcca ctgaagctgc catttttcat ggtttcacca
    13681 tcccagcgaa gggccatcca gcgtgcgttc ctgtatttcc ggctgacgct cccgttccag
    13741 ggataacaca tgttcgcgct cctgtatcag ccgttcctct cttatctcca gttctcgctg
    13801 tataactggc tcaagcgttc tgtctgctcg ctcaagtgct gcacctgctg actcaactgc
    13861 atgacccgct cgttcagcat cgcgttgtcc cgttgcgtaa gcgaaaacat cttctgcaat
    13921 tccacgaagg cgctctccca ttcgctcagc cgctgcatat agtcctgttg cagctgctct
    13981 aaggcgttca gcaaatgtgt ttccagctct gtcactctgt gtcactcctt cagatgtacc
    14041 cactctttcc cctgaaaggg aatcacctcc gctgatttcc cgtacggggt tctaagcggg
    14101 aatcccagaa aattccgtca ttccgtatga tgaaaattca gtcgactcct cgcttgacgt
    14161 gacgagcgcc aaaagcctgc cgcatctgcc tggcgcgctc gagctcatcg ccatgcaaat
    14221 acatcgacgt cgtggcgatc gacgcatggc gtaggttgtc gcgcaccgcg gtgagctgag
    14281 ctccccgggc caacgcatgg ctggcatgac tgtgacgcat ccaatgaggc gtggcacggc
    14341 gcagcttttc ggcggcgacc ggatgatcgg cctcaatggc ctcagcggct tgcgtaaaga
    14401 atcttcgcaa cacgcaccaa aggtgagggc cggtgatgcc ggcattaccg tcttccccaa
    14461 gactgcccac caggggcgtt ttcggattcc accgttcccg gctgactggc agttgacgct
    14521 gtgccaagta ttggtccagc gccgtatacg cgagcggcgg caaggccacc ttacctggtt
    14581 tgccaccttt gccaaccaaa tgcagccagt ggtcaccgtg ctcgtcgagg cggacattac
    14641 ccaggacggc gccgaccagt tcgctagcgc gcaggcccgt ggcatagccg aagtcgagca
    14701 tgaagcgcag gcgttgtgct gccggctcgg accagccgta ggaccattcc aggccgtctg
    14761 caatggcgcg cagcagtagc cattctccct cggtaaagcc tcgcgccgta tccagtgcgg
    14821 gccgcaaggc gtgaccgcgt accttgacgc cggcgaaagg gttggccagc acatagcgct
    14881 gctcgatgag ccaccggaac agcgccgaga gcaccgtcag cgcatacgct gccgagcgtg
    14941 cggacaagcc gcccgagaag gggcgccatt ccaccgaatc gcgtggtcga ggtggcccca
    15001 cccaacgctc gcgcggggtc gggtgccgga caaaggcccg gtacgcgatt gcatcgtccg
    15061 tggtcaggga tgatagcgcc cgtccgcgtt cgacgatggc ccacaggatt agccgcgcag
    15121 cctccttgcg ataggcgcgc tgtgtggcga cagtctcgtg cagcgccagc caggcctgca
    15181 cggcctcgta gtcgttggtc gcattcagcg tgcaagcgtt gcgcggggcc cggaaattgc
    15241 cctgcgagcc gtcgacttcg ttcggcagac gcaactgctc ccacggcacg atgccgctgg
    15301 gctgaataat cgggatcagg gcccgggcgc gctcggtgag ctgcggatgt gcggcaaaga
    15361 aagcctcgac ctgccgggcg cctgtcatgc cgagtccctc gatggctttc caccagccac
    15421 gccggcgggg aatgcgcacg gtcagcgccg agagggtgtg gataccgtac gcctgcagtg
    15481 ccctgacggt cctcggagac agccactggt cgatgctgtc gctgatctgg ggcttcggcg
    15541 ccggcagcgt gggcaagagc cggatcgcgc gttcgacggc acccgcgcgc ccctgtcgct
    15601 cttcgggggc gtgctggaac acgtcggcca gatccggccg gtggcggctg ttggcgaaaa
    15661 cgatgagctg gcggcggata tgccccagca tccctcgcgc tgattgaccg gcggccttgc
    15721 gtgccgccag gtagtgttca acggccgcgc gagagctgag tcccgcgtac cagccgcgca
    15781 aggcagccag cgaatccgca tcgggaaagc cgactggggt agaactcgaa gccgtagtct
    15841 ggcccgtttt catatggaca ggttgcgatt cgaaaagcga gtaacgcgat aataacgatt
    15901 atttcagtag aatcgaaaat gccccatgtt tctacgaaac ctcaagatag caagaacggg
    15961 ccatgagcaa gaagaacaag ctactcagcg tcctatccga cgccgaacag gaggccttgt
    16021 atggcctgcc ggagttcgat gatgcgcagc agctggaata tctggctgtg acagaaaccg
    16081 agttggcgct tgccaacagc cggcccagtc ttcatgccaa ggtctattgc ctcttgcaga
    16141 tcggctactt caaggccaag catgccttct tcagtttcga ctgggacgaa gtcgaggacg
    16201 attgcgcttt cgtgctgagc cgctatttcc acggcgaggt gttcgaaccc aaggcgatca
    16261 ccaagcatga acgctacacc cagcgcgagc agatcgccaa actattcggc tactggccgt
    16321 ggtcggccgc cttcctgccg cagctcgagc agcaggccgc acggaccgtg cggcgtgacg
    16381 taacgccggg gttcgtcgcc gccgagctga tcgtctggct caatgagcac aagattatcc
    16441 ggcccggcta tacgaccttg caagagttgg tcagcgaaac gctatccgcc gagcgccggc
    16501 gcctgggcaa tctgctggag caagcgttgg acgaatctgc caaggccgcg ctgagccag
//
