LOCUS       NZ_CP018943             6647 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_224 plasmid pEC224_5, complete
            sequence.
ACCESSION   NZ_CP018943
VERSION     NZ_CP018943.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511177
            Assembly: GCF_002011945.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002011945.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:33:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,090
            Genes (coding)                    :: 4,879
            CDSs (with protein)               :: 4,879
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 211
            CDSs (without protein)            :: 211
            Pseudo Genes (ambiguous residues) :: 0 of 211
            Pseudo Genes (frameshifted)       :: 76 of 211
            Pseudo Genes (incomplete)         :: 138 of 211
            Pseudo Genes (internal stop)      :: 38 of 211
            Pseudo Genes (multiple problems)  :: 39 of 211
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018943.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC224_5"
                     /geo_loc_name="Viet Nam: Hanoi"
                     /lat_lon="21.02 N 105.83 E"
                     /collection_date="2010"
                     /collected_by="Merck Study for Monitoring of Antimicrobial
                     Resistance Trends (SMART)"
     gene            complement(261..1829)
                     /gene="cea"
                     /locus_tag="BE957_RS00075"
                     /old_locus_tag="BE957_00070"
     CDS             complement(261..1829)
                     /gene="cea"
                     /locus_tag="BE957_RS00075"
                     /old_locus_tag="BE957_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            2089..2526
                     /locus_tag="BE957_RS00080"
                     /old_locus_tag="BE957_00075"
     CDS             2089..2526
                     /locus_tag="BE957_RS00080"
                     /old_locus_tag="BE957_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            2607..3026
                     /locus_tag="BE957_RS00085"
                     /old_locus_tag="BE957_00080"
     CDS             2607..3026
                     /locus_tag="BE957_RS00085"
                     /old_locus_tag="BE957_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(3344..3577)
                     /locus_tag="BE957_RS27760"
     CDS             complement(3344..3577)
                     /locus_tag="BE957_RS27760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3584..4102)
                     /locus_tag="BE957_RS27765"
     CDS             complement(3584..4102)
                     /locus_tag="BE957_RS27765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4140..4838)
                     /locus_tag="BE957_RS28995"
                     /pseudo=""
     CDS             complement(<4140..4838)
                     /locus_tag="BE957_RS28995"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4780..5103)
                     /locus_tag="BE957_RS00105"
                     /old_locus_tag="BE957_00100"
     CDS             complement(4780..5103)
                     /locus_tag="BE957_RS00105"
                     /old_locus_tag="BE957_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5167..5358
                     /locus_tag="BE957_RS00110"
                     /old_locus_tag="BE957_00105"
     CDS             5167..5358
                     /locus_tag="BE957_RS00110"
                     /old_locus_tag="BE957_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6028..6186
                     /locus_tag="BE957_RS29000"
     CDS             6028..6186
                     /locus_tag="BE957_RS29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(6385..6522)
                     /locus_tag="BE957_RS00125"
                     /old_locus_tag="BE957_00120"
     CDS             complement(6385..6522)
                     /locus_tag="BE957_RS00125"
                     /old_locus_tag="BE957_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     Typing          complement(5444..5531)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          5542..6100
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.722"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3241..4790)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aacagcgaag ggtattattt ccttgtgtca gataagatgc tatatgcaat agtgataagc
       61 actattctat gtccatattc aaaatatgct attgaacaca tagcttttaa cttcataaag
      121 aaagattttt tcgaaagaag aaaaaaccta aataacgccc ccgtagcaaa attaaaccta
      181 tttatgctat ataatctact ttgtttggtc ctagcaatcc catttggatt gctaggactt
      241 tttatatcaa taaagaataa ttaaatccct aacacctcat ttatagtatt aagtttattc
      301 ttatcaatat aggagcatag aattcctgta acaatagcaa taccccaaat acctaatgta
      361 gttccagcaa gcaagctaaa aagtaaagca acaacataac tcacccctgc atctgcagct
      421 ttcttctcta atgtaagaaa tagtggcttc cagtcacctg tatccttaat ttttaggata
      481 tcagatacca catcatatcc aaaagaaaca tgccccgtaa tcttcaagta cttggcaaac
      541 tgatctaaat gtttagccca gtcatcatac ttcaccgatg ccaacgcatt aaaaatagca
      601 tcacgatcgg ctttgctgaa tttcttattt aaaacatcct tgtatttttc aaaagcagcg
      661 agagcttcat tcacattgcc gattttctta cctttagact tatcagcaag ttcctgtgcc
      721 attttcgaat atttttcacc atatttttca gtcagcgttt gataaaagct aactgttgca
      781 tcaacagcat ccttaatctg tgaattaagg agattattct gtgctttttt caaattttct
      841 tcagcttcat gaacacgagc gataccggca ttacgattat tactgacctg agaaatagcc
      901 ttctggatct gagttatatc agcatttatc cggttaatac gtgtttctga tgctgttacc
      961 tgtttttgtt tttcttctct aatcttaccg gccccaaccc gtcgtctggt tgcttcaaaa
     1021 aaaggacggt tctgaagcgg atcattggct cttggtgata gttttttgac cagctcatcc
     1081 agttctttat atttagcgga tgcctgagcc agttcatttc gttttccagc gagcgttttc
     1141 atttctgcat cacgggcatg gatactggag cttaaacgag aattgagagt cttaatctct
     1201 ccatccattt tcaccacttc agattgtgca gcagaaagtt ttttttgggc gatctcaaca
     1261 gctttagctt cttcactcaa tgcagccagt cgtttctctt cagcttcagc cagtttcaac
     1321 tggcgttctg tttcagcctt ctcccgttca atctctttac gtcgttgttc tgcttcctga
     1381 aaagcctttt ctgctgcttc cgcttcttta cgggcttttt cttctgcttt cgcaaggcgc
     1441 aaacgctctg cttccgcctg catagctgca ttattagcat gagcaagctc tgttgctgaa
     1501 ggcgtacgtg aggcattgtg acgaagagcc tcattcacga tatccttcag gcgctgagtc
     1561 agcgcatccc tgtttgcctt tgctttcgcc tgtgcttccg ctgcagcttt tgcccgggca
     1621 gcctgctctg cctgtgtttt ctttaattga gcagtagacc atttagcagt tgcatgaata
     1681 gctgcagaac tttcactttt actgcctcct tttccacctc cgccgccaga gccactcccg
     1741 tcaggagtac cattcaaaag agtaataatt acctgtccct tatcatcata aggaacacca
     1801 tctttatagt acgctaccgc ggtttccatt ataaaatcct ctttgacttt taaaacaata
     1861 agttaaaaat aaatactgta catataacca ctggttttat atacagcata aaagctacgc
     1921 cgctgcattt tccctgtcaa gactgtggac ttccattttt gtgaaaacga tcaaaaaaac
     1981 agtctttcac accacgcgct attctcgccc gatgccacaa aaaccagcac aaacattacc
     2041 gttctcagac ctcattatgt tttactgaaa ctatgagatg agacatctat gggacactgt
     2101 cactttatgg catggcacac actccgggac gcactaaaaa tgacaggcag atcgcgttca
     2161 cagttttacc gtgatatgcg cgcaggcctt gtcagttacc gtaccggcag ggacggacga
     2221 cgggagtttg aaaccagtga actgatccgg gcatacggcg aattaaagca gaatgagaca
     2281 ccagaaaggc acagtgaggg acatgcagaa aatccacatg atcagcagac agaacgcatt
     2341 ctccgggagc tgaatgagct gaaacaatgc ctgacgctga tgcttgagga taaacaggca
     2401 caggatatgg atcgcagacg ccaggaagca gaacgggaac agctacaaaa tgagatagcc
     2461 cagctcaggc aggcactgga actggaaaag aaacggggat tctggtccag gttgttcggt
     2521 cgctgaacgc tgtcagagac tgatgataaa atagtcttcg gataataact caccgagaat
     2581 aaatacttta aggtagggag acactcatga gacgtaccgg aaacaaactt tgtcttatcg
     2641 ccatgataac agcaacagta gctctcacag cctgtacccc aaagggcagc gtggaacaac
     2701 atacccggca ttacgtatat gcttctgatg acggttttga tcccaacttt tccacccaaa
     2761 aagccgacac aacacgaatg atggtgcctt tttttcggca gttctgggat atgggagcta
     2821 aagataaagc gacaggaaaa tcacggagtg atgtgcaaca acgcattcag cagtttcaca
     2881 gccaggaatt tttaaactca ctccggggca caactcaatt tgcgggtact gattaccgca
     2941 gcaaagacct taccccgaaa aaatccaggc tgctggctga cacgatttct gcggtttatc
     3001 tcgatggcta cgagggcaga cagtaagtgg atttaccata atcccttaat tgtacgcacc
     3061 gctaaaacgc gttcagcgcg atcacggcag cagacaggta aaaatggcaa caaaccaccc
     3121 gaaaaactgc cgcgatcgcg cctgataaat tttaaccgta tgaataccta tgcaaccaga
     3181 gggtacaggc cacattaccc ccacttaatc cactgaagct gccatttttc atggtttcac
     3241 catcccagcg aagggccatc cagcgtgcgt tcctgtattt ccggctgacg ctcccgttcc
     3301 agggataaca catgttcgcg ctcctgtatc agccgttcct ctcttatctc cagttctcgc
     3361 tgtataactg gctcaagcgt tctgtctgct cgctcaagtg ctgcacctgc tgactcaact
     3421 gcatgacccg ctcgttcagc atcgcgttgt cccgttgcgt aagcgaaaac atcttctgca
     3481 attccacgaa ggcgctctcc cattcgctca gccgctgcat atagtcctgt tgcagctgct
     3541 ctaaggcgtt cagcaaatgt gtttccagct ctgtcactct gtgtcactcc ttcagatgta
     3601 cccactcttt cccctgaaag ggaatcacct ccgctgattt cccgtacgga aggacaagga
     3661 atttcctgtt cccgtcctgc acaaactcca cgccccatgt cttcgcgttc agtttctgca
     3721 atgtctcttc ctgcttcctg atttcttcca ggttcgcctg tatcctccct ccaagatacc
     3781 agagcgtccc gccactcgcg gtaaacagga gaaatactat ccccagtaac atcatgcccg
     3841 tattccctgc cagctttaac acgtccctcc tgtgctgcat catcgcctct ttcacccctt
     3901 cccggtgttt ttccagcgat tcctctgtcg aggctgtgaa cagggctata gcgtctctga
     3961 ttttcgtctc gtttgatgtc acagcctcgc ttacagattc gccgagcctc ctgaactcgt
     4021 tgttcagcat tttctctgta gattcggctc tctctttcag ctttttctcg aactccgcgc
     4081 ccgtctgcaa aagattgctc ataaaatgct cctttcagcc tgatattctt cccgccgttc
     4141 ggatctgcaa tgctgatact gcttcgcgtc accctgacca cttccagccc cgcctcagtg
     4201 agcgcctgaa tcacatcctg acggcctttt atctctccgg catggtaaag tgcatctata
     4261 cctcgcgtga cgccctcagc aagcgcctgt ttcgtttcag gcaggttatc agggagtgtc
     4321 agcgtcctgc ggttctccgg ggcgttcggg tcatgcagcc cgtaatggtg attcaccagc
     4381 gtctgccagg catcaattct aggcctgtct gcgcggtcgt agtacggctg gaggcgtttt
     4441 ccggtctgta gctccatgtt cggaatgaca aaattcagct caagccgtcc cttgtcctgg
     4501 tgctccaccc acaggatgct gtactgattt ttttcgagac cgggcatcag tacacgctca
     4561 aagctcgcca tcactttttc acgtcctccc ggcggcagct ccttctccgc gaacgacaga
     4621 acaccggacg tgtatttctt cgcaaatggc gtggcatcga tgagttcccg gacttcttcc
     4681 ggattaccct gaagcaccgt tgcgccttcg cggttacgct ccctccccag caggtaatca
     4741 accggaccac tgccaccacc ttttcccctg gcatgaaatt taactatcat cccgcgcccc
     4801 ctgttccctg acggccagac gcagccggcg cagctcatcc ccgatggcca tcagtgcggc
     4861 caccacctga acccggtcac cggaagacca ctgcccgctg ttcaccttac gggctgtctg
     4921 attcaggtta tttccgatgg cggccagctg acgcagtaac ggcggtgcca gtgtcggcag
     4981 ttttccggaa cgggcaaccg gctcccccag gcagacccgc cgcatccata ccgccagttg
     5041 tttaccctca cagcgttcaa gtaaccgggc atgttcatca tcagtaaccc gtattgtgag
     5101 catcctctcg cgtttcatcg gtatcattac cccatgaaca gaaatccccc ttacacggag
     5161 gcatcagtga ctaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag
     5221 acattaacgc tgctggagaa gctcaacgaa ctggacgcag atgaacaggc cgatatttgt
     5281 gaatcgcttc acgaccacgc cgatgagctt taccgcagct gcctcgcacg tttcggggat
     5341 gacggtgaaa acctctgata catgcagctc ccggagacgg tcacagcttg tctgtgagcg
     5401 gatgccggga gctgacaagc ccgtcagggc gcgtcagcag gttttagcgg gtgtcggggc
     5461 gcagccctga cccagtcacg tagcgatagc ggagtgtata ctggcttaac catgcggcat
     5521 cagtgcggat tgtatgaaaa gtacgccatg ccgggtgtga aatgccgcac agatgcgtaa
     5581 ggagaaaatg cacgtccagg cgcttttccg cttcctcgct cactgactcg ctacgctcgg
     5641 tcgttcgact gcggcgagcg gtactgactc acacaaaaac ggtaacacag ttatccacag
     5701 aatcagggga taaggccgga aagaacatgt gagcaaaaga ccaggaacag gaagaaggcc
     5761 acgtagcagg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5821 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5881 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5941 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gttggtatct cagttcggtg
     6001 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6061 gccttatccg gtaactatcg tcttgagtcc aacccggtaa ggcacgcctt aacgccactg
     6121 gcagcagcca ctggtaaccg gattagcaga gcgatgatgg cacaaacggt gctacagagt
     6181 tcttgaagta gtggcccgac tacggctaca ctagaaggac agtatttggt atctgcgctc
     6241 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6301 ccgttggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6361 ctcaagaaga tcctttgatc ttttctactg aaccgcgatc cccgtcagtt tagaagagga
     6421 ggatggtgcg atggtccctc cctgaacatc acgtatatag ttagcctgac atccaacaag
     6481 gaggtttatc gcgaatattc ccacaaaaaa tcttttcctc ataactcgat ccttataaaa
     6541 tgaaaagaat atatggcgag gtttaattta tgagcttaag atactacata aaaaatattt
     6601 tatttggcct gtactgcaca cttatatata tataccttat aacaaaa
//
