LOCUS       NZ_CP018942             4063 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_224 plasmid pEC224_6, complete
            sequence.
ACCESSION   NZ_CP018942
VERSION     NZ_CP018942.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511177
            Assembly: GCF_002011945.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002011945.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:33:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,090
            Genes (coding)                    :: 4,879
            CDSs (with protein)               :: 4,879
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 211
            CDSs (without protein)            :: 211
            Pseudo Genes (ambiguous residues) :: 0 of 211
            Pseudo Genes (frameshifted)       :: 76 of 211
            Pseudo Genes (incomplete)         :: 138 of 211
            Pseudo Genes (internal stop)      :: 38 of 211
            Pseudo Genes (multiple problems)  :: 39 of 211
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018942.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC224_6"
                     /geo_loc_name="Viet Nam: Hanoi"
                     /lat_lon="21.02 N 105.83 E"
                     /collection_date="2010"
                     /collected_by="Merck Study for Monitoring of Antimicrobial
                     Resistance Trends (SMART)"
     gene            1..702
                     /locus_tag="BE957_RS00050"
                     /old_locus_tag="BE957_00045"
     CDS             1..702
                     /locus_tag="BE957_RS00050"
                     /old_locus_tag="BE957_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(950..3097)
                     /gene="mobQ"
                     /locus_tag="BE957_RS00055"
                     /old_locus_tag="BE957_00050"
     CDS             complement(950..3097)
                     /gene="mobQ"
                     /locus_tag="BE957_RS00055"
                     /old_locus_tag="BE957_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            3276..3530
                     /gene="mobC"
                     /locus_tag="BE957_RS00060"
                     /old_locus_tag="BE957_00055"
     CDS             3276..3530
                     /gene="mobC"
                     /locus_tag="BE957_RS00060"
                     /old_locus_tag="BE957_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(2740..3098)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3099..3275
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2..702
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(954..3184)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.989"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtcagatc tgatagctta caaatcaaac gcccttatcg aggcaagcta caagctcact
       61 ttgcaggaac agcgcttcct gctggtctgt atcagccgcc taaaatccgg agaagattcc
      121 ccttcgccgg atgaacaaaa aaccatgacc atcacggctg cagagtttca tgatgccttt
      181 cctgacatgg gacggcagca cgctgaagct aggctcaagg aagcgataga tcgcctctgg
      241 gacaggtcag taatcatcaa gaacgaggaa aaacgagaag agttccgctg ggtgcagtac
      301 cgggcgcagt atttcaaagg tgaggcgaaa gtagatatca ccttctccga tgccatcatg
      361 ccgtatctga cacagctcaa ggggcagttt acgcgggtag tcgtgaagaa cgtctccagc
      421 ctttccagca cctacagcat ccgcatttac gagctgctcc agcagttccg cagcaccggt
      481 gacagaacga tagctatcga tgatttccga accatgcttg ccgttgaaga taaatatcaa
      541 caattcaaag acttgaataa gatgataatc aagccagcca tcgccgaact gaacgaaaaa
      601 agcgatctgg ttgtaactgt tgagacgatc aaaaaggggc gcacggttgt agccctgcat
      661 tttcgcttca aggaagacaa acagatcaag atgacgatct aaaatcgatt ttaaggcacc
      721 taaaacgctc cctgctgcgt tttttttgtg ttagtagtgc gaaggtacta ttgaagtgaa
      781 aaattgcgtc aaaaacgctc tgatgcagcg aaaaaagacc ccccaatttt tgggggcgga
      841 aaaacaaaac tatgcttgcg ttatgtgagt gtgcttcttg cattcatccc tgtcgatgaa
      901 catcgacgaa cataacacga tgttaaacaa aacaaaatat tgttcacatt cactgtgacc
      961 aaccgcgatc atgtctcctc tcggtggtgt gtgttcgtgc atcttcctcg cgctgccatt
     1021 cctcccaggt acgattaaaa cccagcgagc ggaacagatg atccggctgt ttttctggtg
     1081 tgttgacggc agcaaaaagc ggtgcctcac gggttttcag atgaaactgc atcagttctg
     1141 gtgcaggtga tatgtggctg tctgcatcgt gatttacggc aacaccatac cctgcatacc
     1201 gtaacccctt atcggtcagc gcactgatga ccagatgcct gttatcggct gagggaacgg
     1261 caaacgcggg atacagcgta ccattaagcc ggataacacc gcccagagag tcatccacgc
     1321 ccatgaccag caaaagctga agctggcggg tttcccggtc gacagaactc atgaccggca
     1381 tcagcgtcgg tggcagacgt ccctgcataa aatccacctt ccttgccgct ggtgatggca
     1441 tggctggctg gcggtcttct ccaagacaga aagactccgg ccatgtcttc ggctggcgga
     1501 gattgcgggt atgttgcggc catgtctggc aaacctgcgt cagcagttgc tcaaaggttg
     1561 cagaggaata aacacgttgc agtggcggac gttggccgat ttcgcttcgg tcacagatat
     1621 aaccactcct gtcacgcatt aaatcaacac tctcaaagat tctggtgcgg tatttctcca
     1681 gctcgttccc ccagagcgta tattctccgt tccggttgtg aagcagaagg taacaggtca
     1741 tttccccgtt accaggcatg ggagcctcgc cataccccgt aacgactgct ctcagcaccc
     1801 ctgaatccat aacggcattg tcatttttcc tgagctgctc cgcgcgacta cgttcagtat
     1861 cttcctgttc cttccggcgc aattcctctt cacgtttaag caactgggct ctgagagctg
     1921 caaggccgga ttccccccac tgctgccact gtttttcaaa tgccatctga ctcgcgggaa
     1981 tgtcctgttt ctccaccttc cggtaatgct gtcgtaattt ttccatttgc tggctcagag
     2041 actggatttt tccctgctca ctacggtatt ccggtgccgc ccattgcatc aggggaatac
     2101 gggaaaaacc ggacgaaaac ggcgatgatt ttttcaggct ggcagcatgg ctcatcgctt
     2161 cctttcgttc ggtgttcagc tcttcccagc gtctgcgcgt cgcctgcata tctgccatca
     2221 tcgacatggc attactggtg agtttccatg ccgagttact gtccttcggc aacgcttcga
     2281 taatgtcacg tagtgtgtac gaagttgcgt tgtcaggaag cccgatattc agaaggtcag
     2341 ccacacggcg ggcaggctct gtcagccctc cgttcctgag ttcggtgcgg gaaatctgta
     2401 tttccaggtt aagcctgtta atcagacgat tctgttcgcc gcgttctgtt tcgataccgc
     2461 gtgtttccat cgcacaggca gactttccga ggtgaatacc aggcgttttc tcaagcccct
     2521 gtcgctcgta tgaacgatgg tctatctctt cctgataacc ggcgttcgcc agtgccctgt
     2581 ttgcatggtc agcccatgat gcccgccacg tctcggcatg tgtccggtcg ttccagtccc
     2641 tgacctttcc gctgaaccca gcaggtccga tagccctcgt ggtcagcatg atgtgcgcat
     2701 ggggatttgt tttatccatg tgatggaacg ccacatcagc aatcatgccc tgactgacaa
     2761 agttttctct gacaaaggcg agaacggttt cccgtgcggc atcctgcggc agttcgtggg
     2821 ggatggcgag ttcaatttcc cgcgcaagtt gtgagttctt ccggcgttcg gccttctcaa
     2881 cggcattcca gagttcggca cggttcgtac accaggcagg ggcggtggcg ggggtcagaa
     2941 tcacagcctc agccacaccg cttttgcggg tgtagtcgtg agtcagtccg gtacgctcat
     3001 caagcatcag tgaaccggaa cgataggcag cggaagctac ggcgctgtag ccgctgcttc
     3061 tggagataat tttcatgctg agatgataaa tcgccatttc tgccgtaatg cctttttgta
     3121 gttttctttc agccccgcag ggcgcacaca atcgaaacgg agtggagatt gtataagtgc
     3181 gcacttcgtg atttgccgtg cattatgcca gctcagtttt taaactacca gtgacgcgct
     3241 aaaatgcgat ttttcatcgc gcacggagtt acatcatgag cagaacgctc gaacagaaga
     3301 ttgctgatgc tgaggcgaga ttacagcgcc tgaaggcgaa gagccggagc ctggacacag
     3361 cgcagaaggt tgttgtgggt gctgcattgc tggcaaaggt cagaaagccg gaggaggtgc
     3421 agttgcgtgc ctggttactc cagttcctga aggcagaggt gacacgacag gctgatgtga
     3481 cgcgcatact gccgctgatt aacgagctgg aggcgctgcc agaacagtga ttgtatcgtc
     3541 cgctcgccgc agccccagcg accgagcgtt agcgagcgag tgggcgagga agcggaagtg
     3601 aatcagtcgc caggttgagc acatacgcac ggtttttcgg caggtgatat gcgctgaacc
     3661 cgcatgaata ctgggtttcc acagggttgg agggggtgta ttcgaaactt gcaattctgc
     3721 tttattgcaa cgcattgttt ttattggttt tttgttttca gcaccccaag ttatccacag
     3781 gctcgccgtt aagtttttaa gatcttattt ttagatcttt ttttttaaaa cctttttagg
     3841 tcttgtaaca ccatgatttt aaagatgttc tggcaagata agtggagctt ttgggggtga
     3901 aaggtggagc ttttgggggt gaaaggtgga gcttttgggg gtgaaaggtg gagcttttgg
     3961 gggtgtaatc cacagaaaaa gggggttgca aaaaaaaaac ttccacccat atgataagtg
     4021 gcgtacagta aaaaaacgcc accaatagca acgagcaagc gaa
//
