LOCUS       NZ_CP018941             4062 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_224 plasmid pEC224_7, complete
            sequence.
ACCESSION   NZ_CP018941
VERSION     NZ_CP018941.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511177
            Assembly: GCF_002011945.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002011945.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:33:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,090
            Genes (coding)                    :: 4,879
            CDSs (with protein)               :: 4,879
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 211
            CDSs (without protein)            :: 211
            Pseudo Genes (ambiguous residues) :: 0 of 211
            Pseudo Genes (frameshifted)       :: 76 of 211
            Pseudo Genes (incomplete)         :: 138 of 211
            Pseudo Genes (internal stop)      :: 38 of 211
            Pseudo Genes (multiple problems)  :: 39 of 211
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018941.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC224_7"
                     /geo_loc_name="Viet Nam: Hanoi"
                     /lat_lon="21.02 N 105.83 E"
                     /collection_date="2010"
                     /collected_by="Merck Study for Monitoring of Antimicrobial
                     Resistance Trends (SMART)"
     gene            join(3712..4062,1..1797)
                     /gene="mobQ"
                     /locus_tag="BE957_RS00030"
                     /old_locus_tag="BE957_00025"
     CDS             join(3712..4062,1..1797)
                     /gene="mobQ"
                     /locus_tag="BE957_RS00030"
                     /old_locus_tag="BE957_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_078207086.1"
                     /translation="MAIYHLSMKIISRNNGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHVHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(2045..2746)
                     /locus_tag="BE957_RS00035"
                     /old_locus_tag="BE957_00030"
     CDS             complement(2045..2746)
                     /locus_tag="BE957_RS00035"
                     /old_locus_tag="BE957_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281397.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3279..3533)
                     /gene="mobC"
                     /locus_tag="BE957_RS00045"
                     /old_locus_tag="BE957_00040"
     CDS             complement(3279..3533)
                     /gene="mobC"
                     /locus_tag="BE957_RS00045"
                     /old_locus_tag="BE957_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(3535..3711)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3712..4062
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.864"
                     /coverage="98.0"
                     /evalue="1.09e-177"
     Typing          complement(2046..2746)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..1794
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.342"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgattgctg atgtggcgtt ccatcacatg gataaaacaa atccccatgt gcacatcatg
       61 ctgaccacga gagctgtcgg acctgctgga ttcggtggaa aggtcaggga ctggaacgac
      121 cggacacatg ccgagacgtg gcgtgcatca tgggctgacc atgcgaacag ggcactggcg
      181 aacgccggtt atcaggaaga gatagaccat cgttcatacg agcgacaggg gcttgagaaa
      241 acgcctggta ttcacctcgg aaagtctgcc tgtgcgatgg aaacacgcgg tatcgaaaca
      301 gaacgcggcg aacagaatcg tctgattaac agacttaacc tggaaataca gatttcccgt
      361 accgaactca gaaacagcgg gctgacagag cctgcccgcc gtgtggctga ccttctgaat
      421 atcgtgcttc ctgacaacgc aacttcgtac acactacgtg acatcatcga agcgttgccg
      481 aaggacagta atgcggcatg gaaactcacc agtaatgcca tgtcgatgat ggcagatatg
      541 caggcgacgc gcagacgctg ggaagagctg aacaccgaac gaaaggaagc gatgagccat
      601 gctgccagcc tgaaaaaatc atcgccgttt tcgtccggtt tttcccgtat tcccctgatg
      661 caatgggcgg caccggaata ccgtagcgaa cagggaaaaa tccagtcgct gagccagcaa
      721 atggaaaaat tgcgacagca ttaccggaag gtggagaaac aggacattcc cgcgagtcag
      781 atggcatttg aaaaacagtg gcagcagtgg ggggaatccg gccttgcagc tctcagagcc
      841 cagttgctta aacgtgaaga ggaattgcgc cggaaggaac aggaggatac tgaacgtagt
      901 cgcgcggagc agctcaggaa aaatgacaat gccgttatgg attcaggggt actgagcgca
      961 gtcgttacgg ggtatggcga ggcccccatg cctggtaacg gggaaatgac ctgttacctt
     1021 ctgcttcaca accggaacgg agaatatacg ctctggggta acgagctgga gaaataccgc
     1081 accagaatct ttgagcgtgt tgatttaatg cgtgacagga gtggttatat ctgtgaccga
     1141 agcgaaatcg gccaacgtcc gccactgcaa cgtgtttatt cctctgcgac ctttgagcaa
     1201 ctgctgacgc aggtttgcca gacatggccg caacataccc gcaatcttcg ccagccgaag
     1261 acatggccgg agtctttctg tcttggagaa gaccgccagc cagccatgcc atcaccagcg
     1321 gcaaggaagg tggattttat gcagggacgt ctgccaccga cgctgatgcc ggtcatgagt
     1381 tctgtcgacc gggaaacccg tcagcttcag cttttgctgg tcatgggcgt ggatgactct
     1441 ctgggcggtg ttatccggct taatggtacg ctgtatcccg cgtttgccgt tccctcagcc
     1501 gataacaggc atctggtcat cagtgcgctg accgataagg ggttacggta tgcagggtat
     1561 ggtgttgccg tcaatcacga tgcagacagc cacatatcac ctgcaccaga acttatggag
     1621 tttcatctga aaacccgtga ggcaccgctt tttgctgctg tcaacacacc agaaaaacag
     1681 ccggatcatc tgttccgctc gctgggtttt aatcgtacct gggacgaatg gcggcgcgag
     1741 gaagatgcac gaacacacgc caccgagagg aggcacgatc gcggttggtc acagtgaatg
     1801 tgaacaatat tttgttttgt tttacatcat gttatgttct tcaatgttca tcgacaggga
     1861 tgaatgcaag aagcacacac aacaaactag ataatctccg catagtttct tctcccccgt
     1921 atatggggga tttttttagc tcacgagagc gattctgacg atgtttttgt gcttcgtagc
     1981 accatcgcat accttacgca aaaaaaacgc gccagagcgc gttttagatg ccttaaaatc
     2041 gattttagat cgtcatcttg atttgtttgt cttccttgaa gcgaaaatgc agggctacaa
     2101 ccgtgcgccc ctttttgatc gtctcaaccg ttacagccag gtcgcttttt ttgttcagtt
     2161 cgtcaatgca gggtcttaat aattgctggt taagtgattt atatgtttga tatttatgct
     2221 ctatatcaag cattgagcga aaatcatcga gagcgataat acgttcgcca gtactgcgga
     2281 actgctggag gagttcgtat atccttatac tgtaacttct tgataatcta gatatattct
     2341 tgattacaac tctagtaaat tgcccttgta gttgtgtcaa ataaggcatt actgcgtcag
     2401 aaaatgttat ttgtgctctg gcctcgcctt tagcgtactg tgcccgatac tggatccagc
     2461 ggaactcctc acgtttctcg tcatccttga gaatgattga cctgtcccac aaacgatcaa
     2521 ttgcttcctg taactgaacc tcggcatttt tccgccccat gtcagggaat gaatcgaaat
     2581 attccgcagc tgtaatggtc attgtctttt gcaattttgg agattctgca tcagccccag
     2641 acttcaatcg accgatgcaa agtaacaaaa atcgctgttc ctgtagggtc aatttataac
     2701 tggcttcaac cagcgcattg gatttatagg caattagatc cgacatagag catccctccc
     2761 caaaaggtaa tgaataaaag ataaacatac cctcaacgaa aggcaatgcc tttttatttt
     2821 aatatccctt tttctgtgaa tattacctta aatcatattg cctttacctt aaatcatatt
     2881 acctaaacac cttaaatcat attaccttta ccttaaatca tattacctat agcgttgtaa
     2941 cattttgttt tttataagga attttggcac taaaacaagt attaaaaaca agatctaaaa
     3001 aacaagatct aaaaacttaa cgacgagcct gtggataact tggagtgctg aaaacaaaaa
     3061 accaataaaa acaatgcgtt gcaaaaaagc agaattgcaa gttccgaata caccccctcc
     3121 aaccctgtgg aaatccagta ttcatgcggg ttcagcgcat atcacctccc gaaaaaccgt
     3181 gcgtatgtgc tcaatcaggc gacagattca cttccgcttc ctcgcccact cgctcgctaa
     3241 cgttcggtcg ctgggactgc ggcgagcgga cgatacaatc actgttctgg cagcgcctcc
     3301 agctcgttaa tcagcggcag tatgcgcgtc acatcagcct gtcgtgtcac ctctgccttc
     3361 aggaactgga gtaaccaggc acgcaactgt acctcctccg gctttctgac ctttgccagc
     3421 aatgctgcac ccacaacgac cttctgcgct gtgtccaggc tccggctctt cgccttcagg
     3481 cgctgtaatc tcgcctcagc gtcagcaatc ttctgttcga gcgttctgct catgatgtaa
     3541 ctccgtgcgc ggtgaaaaat cgcattttag cgcgtcactg gtagtttaaa aactgagctg
     3601 gcataatgca cggcaaatca cgaagtgcgc acttatacaa tcttcactcc gtttcgattg
     3661 tgtgcgccct gcggggctga aagaaaacca caaaaaggca ttacggcaga aatggcgatt
     3721 tatcatctca gcatgaaaat catctccaga aacaacggct acagcgccgt tgcttctgct
     3781 gcctatcgtt ccggttcact gatgcttgat gagcgtaccg gactgactca cgactacacc
     3841 cgcaaaagcg gtgtggctga agctgtgatt ctgacccccg cgacagcccc tgcctggtgt
     3901 acgaaccgtg ccgaactctg gaatgccgtt gagaaggccg aacgccggaa gaactcacaa
     3961 cttgcgcggg aaattgaact cgccatcccc cgtgaactgc cgcaggatgc cgcacgggaa
     4021 accgttctcg cctttgtcag agaaaacttt gtcagtcagg gc
//
