LOCUS       NZ_CP018940             2883 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_224 plasmid pEC224_8, complete
            sequence.
ACCESSION   NZ_CP018940
VERSION     NZ_CP018940.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05511177
            Assembly: GCF_002011945.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Stoesser et al,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Microbiology (Research), University of
            Oxford, Headley Way, Oxford OX3 9DU, United Kingdom (Great Britain)
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: XX
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002011945.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:33:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,090
            Genes (coding)                    :: 4,879
            CDSs (with protein)               :: 4,879
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 211
            CDSs (without protein)            :: 211
            Pseudo Genes (ambiguous residues) :: 0 of 211
            Pseudo Genes (frameshifted)       :: 76 of 211
            Pseudo Genes (incomplete)         :: 138 of 211
            Pseudo Genes (internal stop)      :: 38 of 211
            Pseudo Genes (multiple problems)  :: 39 of 211
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018940.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC224_8"
                     /geo_loc_name="Viet Nam: Hanoi"
                     /lat_lon="21.02 N 105.83 E"
                     /collection_date="2010"
                     /collected_by="Merck Study for Monitoring of Antimicrobial
                     Resistance Trends (SMART)"
     gene            424..537
                     /locus_tag="BE957_RS00010"
     CDS             424..537
                     /locus_tag="BE957_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058651821.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPLAVATGN"
                     /name="hypothetical protein"
     gene            848..1156
                     /locus_tag="BE957_RS00015"
                     /old_locus_tag="BE957_00010"
     CDS             848..1156
                     /locus_tag="BE957_RS00015"
                     /old_locus_tag="BE957_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045351245.1"
                     /translation="MTDKLKGTASVLNQTKTYEELVQKHSPEVANGLLANAINNALPNA
                     GITSNDVAGFSKVTTALRTGEVDLAKTAEEANADAEAVSANILAGLTAKQKSTDEIK"
                     /name="hypothetical protein"
     gene            complement(1260..1895)
                     /locus_tag="BE957_RS00020"
                     /old_locus_tag="BE957_00015"
     CDS             complement(1260..1895)
                     /locus_tag="BE957_RS00020"
                     /old_locus_tag="BE957_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045351244.1"
                     /translation="MNDIFSLIESSRKIKKESIASTVRMPESLHTFIETLACDLELSKQ
                     EIMLKLLEQGARSAQEALAEIEKKELVELGAVEQLEPQTAAGFHILNTNKAHSDEDSEW
                     MLGKGIAAAFYDPWKWNINRIKPNEVVFLYENGKGIVAYGRGTGEVKIRDYHGDKDECH
                     YQELEGFKILENPLSAAQIKKILERNVVFLRTMSAMPDGQKVLNLIEG"
                     /name="hypothetical protein"
     gene            complement(2232..2468)
                     /locus_tag="BE957_RS00025"
                     /old_locus_tag="BE957_00020"
     CDS             complement(2232..2468)
                     /locus_tag="BE957_RS00025"
                     /old_locus_tag="BE957_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016230891.1"
                     /translation="MTRLTNLTPAEKKFIDDAIAAAERAAGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          44..533
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.112"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 aatgcagcta tggcgcgtac ggtgccaaag ataagcccgg agatattccg cttcctcgct
       61 cactgactcg ctccgctcgg tcgttcggct gcggcgagcg gtaccggctt actggcgggg
      121 cggaaatttc ctggcatctt ccaggaagaa gcttaacagg gaagcaagaa ggccacggca
      181 aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat
      241 cagtggtggc gaaacccgac aggacttaaa gataccaggc gtttccccct ggtggctccc
      301 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
      361 gtttatctca ttccacgcct gacactcagt tccgggaagg cagttcgctc caagctggac
      421 tgtatgcacg aaccccccgt tcagtccgac taccacgccc gttccggtaa ctatcaactt
      481 gagtccaacc cggaaagaca cgacaaatcg ccattggcgg tagccactgg taactgattt
      541 agaggaggag agtcttgaag ttcctggtgc gatgcagcta cactgaaagg acagttttgg
      601 tgactgcgct cctccaagcc agttaccttc ggaagaaaga gttggtagct cttgattccg
      661 gcaaacgaac caccgttggt agcggtggtt tttttgttta cagggaatga gattacgacg
      721 agagtaaaag gatcacaaga agatccggct gtttagcagt gatttggctt cagtatgagt
      781 gcccgcctaa cgcgagtctt gtacataaaa ttttctactc ctgactcatc acagactaga
      841 ggtttacatg acagacaaac tcaaaggaac agctagcgtt cttaatcaaa ccaaaactta
      901 tgaggaattg gttcagaagc attcacctga agtagctaac ggcttgctgg caaatgctat
      961 caataatgct ttgcctaacg caggaataac ttcgaatgac gtcgctggat tcagcaaagt
     1021 gacgacggcc ttgcgtacag gagaagttga tctagcaaaa acagccgaag aagccaatgc
     1081 cgatgcggaa gccgtttcag caaatattct tgctggcctg acagcaaaac aaaaaagtac
     1141 agatgaaata aaataatttc ccgcaggtac aaaaaagccc gaactagacg ggccaaggtt
     1201 tcgtatgtaa ttacttataa aaaaatttta accgcactca accccatcaa gtgctcggct
     1261 taaccttcga taaggttcag caccttttga ccatctggca tagccgacat tgttctcaaa
     1321 aacacaacat tccgctccaa tatcttctta atttgagcag ccgatagcgg attttcaaga
     1381 attttaaacc cttccagctc ttggtagtgg cattcgtctt tatcgccatg ataatcgcga
     1441 attttaacct caccagtacc cctaccatag gcaactatgc ctttaccgtt ttcatacaga
     1501 aaaactactt cattgggctt gattcgattg atgttccatt tccatggatc ataaaaagca
     1561 gcagcgattc ctttgccaag catccattca gagtcttcat cgctatgagc tttgtttgta
     1621 ttcaaaatat ggaagccagc ggctgtctgc ggctcgagtt gttctacagc gcctaactca
     1681 accaactcct tcttttcaat ctcagctaac gcctcttgag cactcctggc tccttgttca
     1741 aggagtttaa gcatgatctc ttgctttgaa agttcgagat cgcaggccag cgtttcgata
     1801 aacgtatgca acgactcggg cattcgcact gtggaagcta tcgattcttt tttaattttt
     1861 ctggagcttt cgattaaaga aaaaatgtca ttcattttat gaaccttaaa ttgggttggt
     1921 tatagaagca tatagcgaat caaaaaacga tgcaataatt gaatcaaaat agattctaac
     1981 tccaggtgat agatgatggc gtatgcacga atagtcatta cgatgtgtat ttaccataac
     2041 atccgttatc agcaccccct gacaggccct cagcgcaatt ctggcgcatc agagccgttt
     2101 ccggcacatt tcctcacccg catcccggaa aaacgcccct gatgatttct gcgcgactgc
     2161 atggctatgc acgcgggtga atcgcaggtg atttcgcagc gtcagcacca ctaaaccgcc
     2221 aaaatccgca atcagcggcg cggcgcgcgc ggacgtgacc aggcaaaatc ggactgctgc
     2281 cgctcatcct cacgtaatgc acgctggcgg tcggcgtaac gctgcgactc aatctgcgca
     2341 cgcgctctgt tcaggacgat gtgccggtta ggctgattga gcttcttccc ggccgcacgt
     2401 tcggcagcgg ctatagcgtc gtcgataaac tttttctcag cgggggtcag gtttgtcagt
     2461 ctcgtcatgt ttatctccgt atgagaatag tccctgcagt ctgccagaca cagtaggaac
     2521 gatgaagcgt aagcgacggt gaaaggcagt cagaaacggt gaacgggagc ggattttaat
     2581 ccggtgccgt gaggcgttgg acaagccgca ggcgcgtcag tggatttagc gggtttcggg
     2641 gcgcagccct gaaccagtca cgtagcgcta gcggagtgta tactggctaa acttcagaga
     2701 tgccaaggaa gggacaggat atgttttgta gcaccgccac cacggtggcg atactccaaa
     2761 acgcctggtc agggcacagc cccgacaccc cagagccgac tgtaggtcac tgggcgaacg
     2821 cggcggaatg atgtcggatg acgtcatttt gcggcgtttg ccctatcctg catcgtatta
     2881 aaa
//
