LOCUS       NZ_CP018829            13141 bp    DNA     circular CON 03-APR-2024
DEFINITION  Enterococcus faecium strain ISMMS_VRE_12 plasmid p3_ISMMS_VRE12,
            complete sequence.
ACCESSION   NZ_CP018829
VERSION     NZ_CP018829.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03988631
            Assembly: GCF_001953235.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13141)
  AUTHORS   Bashir,A., Attie,O., Sullivan,M., Sebra,R., Singh,K.V., Altman,D.,
            Pak,T., Dutta,J., Chacko,K., Webster,E., Lewis,M., Hamula,C., Delli
            Carpini,K.W., Murray,B.E., Kasarskis,A., van Bakel,H. and
            Huprikar,S.
  TITLE     Genomic confirmation of vancomycin-resistant Enterococcus
            transmission from deceased donor to liver transplant recipient
  JOURNAL   PLoS One 12 (3), e0170449 (2017)
   PUBMED   28301471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13141)
  AUTHORS   Bashir,A., Attie,O., Sebra,R., Singh,K.V., Altman,D., Pak,T.,
            Sullivan,M., Chacko,K., Lewis,M., Dutta,J., Hamula,C., Delli
            Carpini,K.W., Murray,B.E., Kasarskis,A., van Bakel,H. and
            Huprikar,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2016) Genetics and Genomic Sciences, Icahn School
            of Medicine at Mount Sinai, One Gustave Levy Place, New York, NY
            10029, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP3 v. v2.2.0
            Genome Coverage                   :: 278.48; 196.4
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001953235.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 02:06:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,103
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 45 of 132
            Pseudo Genes (incomplete)         :: 76 of 132
            Pseudo Genes (internal stop)      :: 34 of 132
            Pseudo Genes (multiple problems)  :: 19 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018829.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="ISMMS_VRE_12"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p3_ISMMS_VRE12"
                     /geo_loc_name="USA: New York City"
                     /lat_lon="40.7127 N 74.0059 W"
                     /collection_date="2011-05-05"
                     /collected_by="Shirish Huprikar"
                     /note="Vancomycin-resistant Enterococcus faecium from an
                     infection of the liver of the donor of a liver transplant"
     gene            join(13141,1..689)
                     /locus_tag="AL026_RS16715"
                     /old_locus_tag="AL026_16755"
     CDS             join(13141,1..689)
                     /locus_tag="AL026_RS16715"
                     /old_locus_tag="AL026_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347611.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            1541..2473
                     /locus_tag="AL026_RS16725"
                     /old_locus_tag="AL026_16765"
     CDS             1541..2473
                     /locus_tag="AL026_RS16725"
                     /old_locus_tag="AL026_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002313300.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            2490..2960
                     /locus_tag="AL026_RS16730"
                     /old_locus_tag="AL026_16770"
     CDS             2490..2960
                     /locus_tag="AL026_RS16730"
                     /old_locus_tag="AL026_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002302544.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            3044..3361
                     /locus_tag="AL026_RS16735"
                     /old_locus_tag="AL026_16775"
     CDS             3044..3361
                     /locus_tag="AL026_RS16735"
                     /old_locus_tag="AL026_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_002302541.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKIMVKNYGKKSYKNTNTIISFCNFFKFSFHLNSVRILL
                     F"
                     /name="helix-turn-helix transcriptional regulator"
     gene            3651..4832
                     /locus_tag="AL026_RS16740"
                     /old_locus_tag="AL026_16780"
     CDS             3651..4832
                     /locus_tag="AL026_RS16740"
                     /old_locus_tag="AL026_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /GO_process="GO:0051301 - cell division [Evidence IEA];
                     GO:0009252 - peptidoglycan biosynthetic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="WP_002302539.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVR
                     ESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHV
                     FENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            complement(5061..6209)
                     /gene="tnpB"
                     /locus_tag="AL026_RS16745"
                     /old_locus_tag="AL026_16785"
     CDS             complement(5061..6209)
                     /gene="tnpB"
                     /locus_tag="AL026_RS16745"
                     /old_locus_tag="AL026_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(6226..6630)
                     /gene="tnpA"
                     /locus_tag="AL026_RS16750"
                     /old_locus_tag="AL026_16790"
     CDS             complement(6226..6630)
                     /gene="tnpA"
                     /locus_tag="AL026_RS16750"
                     /old_locus_tag="AL026_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            complement(7167..7628)
                     /locus_tag="AL026_RS16760"
                     /old_locus_tag="AL026_16800"
     CDS             complement(7167..7628)
                     /locus_tag="AL026_RS16760"
                     /old_locus_tag="AL026_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000389746.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            7761..8135
                     /locus_tag="AL026_RS16765"
                     /old_locus_tag="AL026_16805"
     CDS             7761..8135
                     /locus_tag="AL026_RS16765"
                     /old_locus_tag="AL026_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000713874.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            8189..10315
                     /locus_tag="AL026_RS16770"
                     /old_locus_tag="AL026_16810"
     CDS             8189..10315
                     /locus_tag="AL026_RS16770"
                     /old_locus_tag="AL026_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002289343.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            10312..10932
                     /locus_tag="AL026_RS16775"
                     /old_locus_tag="AL026_16815"
     CDS             10312..10932
                     /locus_tag="AL026_RS16775"
                     /old_locus_tag="AL026_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            11046..11183
                     /locus_tag="AL026_RS17625"
                     /old_locus_tag="AL026_16820"
     CDS             11046..11183
                     /locus_tag="AL026_RS17625"
                     /old_locus_tag="AL026_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            11625..12005
                     /gene="mobC"
                     /locus_tag="AL026_RS16790"
                     /old_locus_tag="AL026_16830"
     CDS             11625..12005
                     /gene="mobC"
                     /locus_tag="AL026_RS16790"
                     /old_locus_tag="AL026_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            11987..12901
                     /locus_tag="AL026_RS16795"
                     /old_locus_tag="AL026_16835"
     CDS             11987..12901
                     /locus_tag="AL026_RS16795"
                     /old_locus_tag="AL026_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002305249.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            12861..13139
                     /locus_tag="AL026_RS18020"
                     /old_locus_tag="AL026_16840"
     CDS             12861..13139
                     /locus_tag="AL026_RS18020"
                     /old_locus_tag="AL026_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     BGC             2761..13135
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1542..2473
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          11988..12898
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggcatttta tttaccctat ctactcattt ttgttagtat ttcaggaagt atttggctca
       61 tctataaaat ttttcaaaca agatattcat taaaaggctc taaaattcga tttaagcgat
      121 ttttctttct tggctgtata ttttccttga taatcgtttc aagcggctta ttcggcgttt
      181 tagagggaaa taagcgcgtt tctagaagta ttttattagg aaatgtcact caaaagtatg
      241 aatcagcgcg tgataaaaag aaaaaagagc aagcattagc acaaaaaatg gaagagttta
      301 ccacttgtta tgaggaaatg aacgatattt ttgtgaatca agaaaaacgt ttgacggata
      361 aaaacatgga aaaactcact cgtctttatc aaaacttgcc agaaaaacag caaaaagagg
      421 ttcaagacaa ctatgagcga accaaaaaag acgtgcaata tgtgaaagat acgaaagtgg
      481 aagaagcttg tagtgattta tttggagata caattccttt ttcaactagc gaacaagaac
      541 gaaaagaacg acaacaatca gtcacatatg aacgctatga aatcttgctt caacaagcaa
      601 ttaacataca aaatctgact aaaaaagaaa cagctttaaa ttatttaaaa agcgtcaagg
      661 aatggttaga tcagcaacag caaaattgaa tttgattgaa attcatgtta aactaaacat
      721 gcaaatgttc aaacattaga tacacgaaag ccccagagag gtcggattct ctggggcttt
      781 ttttaggttg atgtcgcctt tactgatttt acttcttgtt tcggttattc aaccaataac
      841 tgaatacaga gatacaaact cctacaagaa gggggcaaat aaacagttca aacatcagat
      901 acacctcctt ccagtcgcaa gactgtcgga taggcgacaa gttattatac cctacaaaat
      961 taaatctgtg tggcaatttt aagtatctct tatttccggg ttgttagggc ttgccctgac
     1021 cgcctgtagg gcgctttaaa ttccagataa agttttttat actaagactg tcttaaaacg
     1081 gcttagacaa gctcttagag cctcaaccct cctttttgaa caatggtttt agcaaaggag
     1141 ctggaggcga ctgcgcaggt ataatgtgag agcgtagcga tcacaccttt ttggcgaccg
     1201 tagggagcga ggttttgaat tagcagttta gaaaagttcg cgaacctgtt taaagaaaaa
     1261 ataattgttt ttttaggtgg gaaattttgt ctccctcttt tataatcttt ttttaaacct
     1321 ctttttaaac ctctttaacg tcggctgtgt ccttactccc ccaaggtata caaaatttta
     1381 taggcatgaa attatctgtt tataggcatg aaattatctg tttataggca tgaaattatc
     1441 tgtttatagg catgaaatta tctgttttag gtattgtttt tagacataat atgtcctaaa
     1501 atgatgtcaa taaaatcatt tagactggag taacaactaa atggcaaatg aaatcgtaaa
     1561 gtatgaacca gaattaaata ccattccttt aagaaaattt accccaatag aaatgaactt
     1621 gtttttttct attgtctcta gaatgcgcga taaaggtaat caaaaaatac gcttttattt
     1681 cagccaactt aaagatttaa gtgcgtataa gcagactgca ggagaagtct ttataaaaga
     1741 tttggcaaga acatacgatc atttgatgga tttacgtttc ggaagaaaaa gcaatggtgg
     1801 attaacaata gaacgattta caatgtttac tgattttgaa attcatggag aagtcgataa
     1861 cccttatgta gatataaaag tccacgaaaa agctttgcct ttacttaata atctagaaag
     1921 ctgggtacgt ttctctttaa cagaatttcg agatttaaat agcagttatg ctaaaacaat
     1981 gtttagattg ctaaaacaat accgaactca aggatgggct gaattttcta aggaagattt
     2041 tttagagcta ttagatattc ctaaaagcta tagacaaaca aatataaatc agtttgtact
     2101 caaaccaata aaagaagaac taactccact atttaaagga ttaactattc gtaaaaaata
     2161 tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt acttggaaag ctgaaatcaa
     2221 ccatgctgac gatttttcaa aagggaagca agaagattta cgtataaaat tatttaatat
     2281 tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt gctaaagatc gaatactaaa
     2341 tcttccgtta ggtactcatg aagctgattt taaaaaacaa caacaaaccg aaaaagaaga
     2401 agcggaaaaa caatcaatct caaatgagct taaacaagat ttattagaaa acttacaaaa
     2461 cttattcgat tgaagcataa ggagtattta tgatagataa aacgatcaaa gaactcgcgg
     2521 aagaatttca tgtttcaaaa caagcaatta ggaaaaggct atcagctgat tttagagaaa
     2581 actatgtgca aactgttact aggaacggag tgaaaacctt agttgtcagc tacaaaggat
     2641 atttgctttt aaaacaacat tttttaggta gtaacaccac tggtaaccaa gtagaaaccg
     2701 ttgctagtaa cgatgaaaac tcgattattg tttttttaca tgaacagctc caagaaaaag
     2761 atactcaaat aaaacaaatg caaaagttac tcgaccagca acaacaactc accttgcagg
     2821 caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt agcttatacg gaagaacctg
     2881 aagaagagga attaacttct gtcaataaag aaaacaacaa tgcaataaca acagaaaaaa
     2941 agtggtggaa attttggtaa caattaccaa ctttccataa agaagaaatc aatttctata
     3001 gactctatct ttctaagaat aaaaaatatg tttaggtgat caaatgacta taagtaaagt
     3061 aatcaaacaa aaaagaatag aaaaacaact aactcaagaa gatttagctg aaatgctttt
     3121 agtatctaaa aaaaccattt ctaattggga aaatggtcga acaataccag atacagaaaa
     3181 tttaattcag tttgctaagt tatttgattt atcactagac aatattttgt taggagaaga
     3241 aaaaattatg gtaaaaaatt atggtaaaaa atcatacaaa aacactaata ctattatttc
     3301 attttgtaat ttttttaagt ttagttttca ccttaattca gtccggattc ttttatttta
     3361 gccattcaat tgccacttgg ttattaatat tttttctaag tttactatta ataattaata
     3421 cattaataat taatacttta attattatct ttaataaaca tagaaactta tttcttcaca
     3481 gcatatttcc aattgtttta ttaatttttc tatactttat ataaaaagat agaactattt
     3541 agtaagataa aaaagtagct aattgtaatt ttcgtctaaa actcaaaact agtaaaaata
     3601 actttattgt gttattttta tttataaatt atgaaaaagg acctttcgaa atgaaaaata
     3661 atagaatcta caaaaatcag ttgaattatg ggcttttatt gccagttttt ctgctatgta
     3721 tcattggatt actttcttta tatgttgctt tatatcacga ctcttacact aattcaaaaa
     3781 tcacaaaaga attaattaaa caagtattgt ggtatagtat tgggttcact tcagcaataa
     3841 ctatttattt ttttaatcct aaaattttgt ggaaattaac tcctatattt tacggattat
     3901 gcatcataat gttattactt ctcttgaaat tctataatcc acaattagca gtcttaactg
     3961 gagcaaaaaa ttggttttat attgctggtt tttcgtttca acctactgag ttagtcaaaa
     4021 taggatacac ccttatgcta gctaaaataa ccgtcaatta tcagcaaaaa tctattacta
     4081 gtaatacgaa agatgattgg atttatttac ttaaaacttt tattgctact cttccaatag
     4141 gaatattatt attttttcag aacgatctag gaacaatgtt agtcttcatt gttatttata
     4201 tttttatttt gtttacgtct aacataaata taaaaataat tgccccaata atcgccattg
     4261 gtgttgtttt atccttaata tttttatatt tggttatatc agatacagga agagatttct
     4321 tattaaaaat aggatttcac gactatcagt ttaaaagaat agactcatgg ttgaatccat
     4381 ttttagatcc aaatggatct agctatcagt tagcaaatag tttaattgct attggctctg
     4441 gcggactttt aggtactggt tttaatgtat caaacattca cgttcctgtt agagaatccg
     4501 atatgatatt ttctgtaatt ggagaaaatt ttggctttat aggaagttgt cttgtaatat
     4561 tcctatactt ttatttaatt tatcaaatgt ataaaacatg ttttaaaagc aataacttat
     4621 ttttcaccta tatcggtata ggcattgttg caatgatttt ttttcatgtt tttgaaaata
     4681 taggtgcaag tatcggatta ctacctctaa ctggtattcc actcccctat attagtcaag
     4741 gtggttctgc cattcttagt aattttttgg ggttgggatt tatgttgtgt tgtagtagat
     4801 atactaacga aaaaaaagga acacaaaaat gaattcaaaa taacacttta tgaatagaat
     4861 gtctatttca ataaaaatta tgatacctta aagttaatca gtgaactact caccacttag
     4921 ctaaacctga cggttcttaa cgcttgaagt gggagcttct tgggaataga gcatacttga
     4981 tagcgtattt ctttactaac agcggtgtct cttatttacc aagctatccc cgtagttcct
     5041 acggttcttg atgattggat ctaagccaat cccatagaat gtagtcttag accttcggtc
     5101 agaatattga tactagcatt gatatcacga tcatgatgag tatgacaaac aggacaagtc
     5161 cattctcgaa tttcaagaga tttcttgcca tctttatgtc cgcactctga acaaatctga
     5221 ctagacggaa accatttatc tactttgatg atttttcgtc cataccagtc agccttgtat
     5281 tgtaatttag tcacaaactt tgaccaagaa acatcagaaa tacttttagc taatttatga
     5341 tttcgcaaca taccttttgt gtttaagtct tcaatacaga taatatcgtg atttttgatc
     5401 atttctgtac tcaacttatt cagaaaatca gtacgttgat tcattacttt ttcatgcaat
     5461 ctagctactt tgcgtttttg tttttgataa tttttagctt caaatagatt ggtaccttta
     5521 tttttagcca ataaagcacg cctagacaat ttgcgttgtt cacgctttag tttcttttcc
     5581 attttggacg tgaatttatt attatcaatt ttttgtccat cagaaagaat cgcaaagtcc
     5641 ataataccta agtcaatacc aattgtggaa ttagttttag gtaattcgat aatttcttct
     5701 ttacacaaca aagaaatata gtatttaccg ctagaatgac gtgatattgt agcggatttg
     5761 ataatccctt ttggttgtct atgaagctta attcttacta atgacttcaa tttaggaact
     5821 ttgatgaatt tactatcaat caaagcaact gtaccatttt gattattcgt tgtataactc
     5881 tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc ccgaaaaaaa
     5941 ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct atcaatttct
     6001 ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat ttttttagaa
     6061 gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag attgtagacc
     6121 ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt tggatagatt
     6181 ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt tatgattgtt
     6241 tccttgattt tggatatact tcttaatcac atcaattggt gcgccaccag ttgtcagaag
     6301 acaaaaactt ttagaccaga acatttcttt ccaaagaaat tgtttgactc ttggaaagtc
     6361 acgttttatc aatcttgagc tggcactttt ataggcattg atgaattttg tcaattctgt
     6421 tttaggttga gctttgaaca taatatgaac gtggtcttta tcatgattcc actccactaa
     6481 agtaatgtga tacgattctg aaattctttc aaaagtagtt ttggcataat cagatatttc
     6541 atcatcaatc acttgtctac gatatttcgt tactaaaaca aggtggtaat aaagaagaaa
     6601 tactgaatga ttatttgtat ctaattccat gttatatcaa ctcatttctt gtatagactg
     6661 attgtaacat ggaacaaaat aaaaaggcaa tacgccttag ttgtcggtct tcgagttacc
     6721 aacggtaacc ctcataccga acggcattca tcacccacct atagaggatg ggcgacttct
     6781 gcctaattct gttaaattca aattattaca acagtcctaa cgaagaattg tttgtaaaac
     6841 aaacggataa ataataaaaa agactgctct ttcgagcagc cagttaaaaa ataatgagtg
     6901 tttattcttg aaaaagcaac gcgcttatga agcgctacca aaaatttatc atattttatg
     6961 aaaaataaca ccccagtaaa aaatgactgg taaaaattca caaagaaacg gaaagaagca
     7021 actgcagaaa atcaagcctt aaaaagagct tttgacctag accaatgaaa aatcacaaag
     7081 tgagcccctg tatcccttgg tagagaggct acttggcata gtcgacatta tgtcaactat
     7141 gcaattaaat aactaactac ttcatattag cttaataaaa taatcaaaat cactagggga
     7201 aaaaatattg tataaaaaaa tttttcattt tttaaataag aatagtaaat ataaaaataa
     7261 tttaagatat ctattactat taatccagat agagcaaacc aagtaaatat tccgtgtgta
     7321 aagctaaaaa aattcatttg aatcaattca aataaaccta atatagtaac aaatatatta
     7381 aaaataatag ctgtcaatac cagtaaatta ttttgtttat tttttaaatt atcttcattt
     7441 aaaatagaat ttatgttagt actataaaat tcacaaattt tttttagtgt ctctatatct
     7501 ggtgttgttt tgttattttc ccactttgaa atagtttgac gtgtaacatt aaattttctt
     7561 gccacatatt cttgagtata cccttttttc actcttaatt ctcttagctt agatcctaaa
     7621 tcctccattt tttctcctat gtaaacgatt atttacataa aattttatca taaaaatact
     7681 aacgcgccaa aaaagttctt tattttataa aacacactaa aataaaataa ttctagtaaa
     7741 aaagatagga gaaataagtt atgaaaaaat ttttatgtct ttctgcttta atgggtgtct
     7801 tattaactag tggaggaata gttagtgcta cagaagcttt aaatctagat gtacccgaag
     7861 accataccgc catttacgga ggaggaatgg aagctacaga agaaggattt tcttctagta
     7921 aattgagata tgctgctggc ggcggagatt tcaactgtgg ggttaatgga tttaaagttt
     7981 atgcaaatta ctaccatgct agagctaaac atagtgcaac agctaaaaat ggacgaggcg
     8041 gtcaagtacg atcagttcag aaagctggag taagagctta tgcaacatgc aatgccacac
     8101 ttactggaaa tactggttgg tggaatgttt attaaaaaag agagtttaaa ctctcttttt
     8161 tatttacatt tgaaaggaaa ttcaaaatat gttaaaaaaa ataaacgctg ttttatcact
     8221 ttttgtctgc attacaatac tattcttttc tactaatgtt ataaatggta taaagacttc
     8281 tgaagcgcta tataaaaata ccgctgaaat tcatatgcaa tataaagatg atatggactt
     8341 taacagttat ataaacgctt tgtatgaaat atctacacaa gaaaatatta atatttctca
     8401 atatagtttt actgctgtta accaactaac tattcttgct acaaacccta acgctaacaa
     8461 acactggaaa ttttctaaaa aaaatatatt agcaagaaac gattctaaag tccattatac
     8521 taataacaaa aattctaagt accaattaaa acttccaaat aattttttaa atataaatat
     8581 ttatcctttt tctcatgtgg aaaatattgg attaagtgaa atattatatg tacaaggaaa
     8641 ctcaaacaaa ttaattcctg tactgaaaaa atatggaaca cctactattt ccaatatcaa
     8701 tcaatcagat acttttcaaa ttaatactaa tattgttctt gttatctgct atcttagtat
     8761 atttattgta attactacaa ttatatttgc tttttcaaaa atgaaagaaa tcactctaaa
     8821 aaaaatgtta ggctataact cttttgatac tataaaagat tctattaaag agttaaacct
     8881 cttattttgt tctttgatta taactttcat tttatctatt tttttgagca tatattacag
     8941 taacgaaaat tttttcctat catttcttct atgtagtatt tttcatttta taattagttt
     9001 tttatttatc atagttactc taagtatcta tcatttatct agtataaaaa attatctaaa
     9061 aaattcacga ccacttaagt taaatctttg tatattaaat atctgtctat tcttaagcat
     9121 gatccttctg ttaatagctt ctacaaaagt aataaatatc cataatgaag ccgaaaataa
     9181 ttctcttaaa tattgggaaa gaactactaa tttatataaa acaaatatta ctaatcaatt
     9241 aaatagaaac aacaccgtag aagaaaataa ctacttgaaa aaagctagta aatttgttta
     9301 taaaatacag aagaattata aaacttttat tatcgcgcca tacaattacg caactatcca
     9361 agaaaataat aaagaatttt ttattggaca aaaagtttat ccaaattttg aagactatgt
     9421 tagtcaacct gctggtgcag gaatatgtgt tgatttaaac tatctaaaat attacaatcc
     9481 tatttctttt gaagaatcta ctgacttaaa tttaataaac tctgatccac taactactta
     9541 cattctagta cctaaaaaat ataaagaata tgcaaaaatg attgaaaaaa attatttaga
     9601 agacatacag tttagacttg aagactctcc tcctcaagct ccttataaaa ctcctaattt
     9661 gaaaaattta aaaataaaac taatatttgt taataataac caaaagtatt ttagttttaa
     9721 taccttatat ggaaaggcaa aagataacta tacaataact gatcccatag ttagagttat
     9781 taatccagaa ataacagaat cattattttg gggaaatata ttgactagtg atggtggatt
     9841 attttttgat cagaaacata cttcaaacca aaactttttt aatcaaatta atccgtatat
     9901 aaaagaattt aacctagaaa atatcataaa tttttcttat tctgtttttc aagaaactgg
     9961 tgaattatta tctcaaaaaa aagtaaataa attaaatgca tatctacaat atcttttaat
    10021 attaggatta tttataataa ttaattttca aatgattttt gttagtttta ctataaattc
    10081 aaaaaaaata gctttaaaaa aaatatttgg atattcacca ataagtcaag ttttagattt
    10141 atgtttactc ccttttacaa tagagttatt tgctactatg tgttttcaat tgatattttc
    10201 aaaaaacact tacttgtatt ttataatttt tatgatcttt ttaataaatc taattacttc
    10261 tattattatt tataaaaaaa atagttttaa atattttaag gagaatattt tatgatatta
    10321 tcagttgaga atttcaccct gtcaattaat aaaaaaaaga tcaaacaaaa ttggaacttt
    10381 tcaatatcag aaaaagattt tattatagta acaggaaaaa gtggtagtgg aaaaactact
    10441 ctgttaaata atttaagcct aatagataaa caatataccg gtaacttatt ctattttgaa
    10501 aagaaagttt ctaaacatga tatacaacgt ctacgaagaa atcatattag ctatttattt
    10561 caaaactatg ctcttcttga aaatcaaaca gtacaataca atttcgaatt agcaaaaaag
    10621 tttaacagta atatttccaa tgatcatatt tataatttat taacagattt ttctttgcca
    10681 aaagatatct taaaagaaaa aatctttctc ttaagcggtg gagaacaaca aagaatagca
    10741 ttaattagaa atatgttaaa accatttgat atattatttg cagatgaacc tacaggaaat
    10801 ttagataaag aaaactcaac ttttataatg aactttctga aaaatttaac agaaaaagaa
    10861 aaaaaagccg ttgtactagt aacacatgac ttaaatgttc taaactatgg aactaatatt
    10921 atagaattat aatttttacc cgtgatgcta gctgatttaa aactagcacc acgggtattt
    10981 attttatatt ccatctctcc tatcccaaaa tttaaataat cacttaataa aaaggaggca
    11041 cccaagtgat tttattgaaa aaagagtatg aaaaatttta tgatagactt atttattctt
    11101 tgcaattgcc aaaaaaatta aaccgtcaat tacaaaagac ttatgaaact aaatcaactg
    11161 aagaatggga gagttattgc tagatttttg gtagaagtaa caaaaagtgg acatattatc
    11221 ataatgactc gccgaaagtc tgataaaaaa ttggctaaaa atattgctga atgtttaata
    11281 aaactatcag ttttagctaa atagttagta gtttacacaa aaaagcattt atgttttttg
    11341 ttgcaaagca acaatttcat cttcggtgaa ataagcgaaa ctttacaaaa agtggctagc
    11401 atttgctagc tttttttgta ttgtggagcg tggcttttga gggggattca agggggagct
    11461 tgcgcccctt tgacaagcgg ttatttgcac caaatacgga ggtatttggc tagcaaatat
    11521 agagcttgcc aaaccctttt agtgagcttt actcattgta cctttttagg tacaatggta
    11581 ttgcgaacga tacgtccgta ggacgtactg aaagggtggg acttatgagc gaacaaaatc
    11641 aaaatttggc tagcaattct tctaaaaaat atacctatcg atccgagcca aaacaaatca
    11701 gttttcgagt gagcgaatcc gaatttgcaa agctgaagca atcggctgaa gctttgcaaa
    11761 tgagtgtgcc ggctttcgtg aaagccaagg cacaaaacgc gcgtctggtg acaccgaaag
    11821 tggcgccaga cattgcccag gcaatcgcgc gcgatttagc caaagctgga gggaatatca
    11881 atcaaattgc taagtggtgc aatacacacc aacatgacgt tgcttctggt gacgctcaac
    11941 gtttatccga aaacctaaaa atcatgcaaa aggagctaca aaagatatgg caacaattaa
    12001 ggtaagccga accacttctt gtagtcgtgc aattaattac gcagaaccac gagcaaccat
    12061 taaaacagga ataaactgtg acgtcgatta tgccaaaagt gaaatgaaac aaattcgcat
    12121 gctgtatgga aaagacgacc gtgtacaggc acacttattg attcaatctt ttcgaccagg
    12181 agaaattacc gctgaaaaag ctaatcaact aggggcagag tatgcaaaaa aaatagcccc
    12241 cgatcaccaa atagctattt acacacatac agacaaagac catattcata atcacattgt
    12301 cattaactcc gttaatctgg agactggaaa aaaatttcaa gctcatggtc aagcatttct
    12361 aaacaaatgt tatgacctaa atgacgagat ttgtcttgca cataacttac caatcactga
    12421 gcgaggaaag aaaccagaaa aacgaaccat gtctgaaata aaattaaaag aaaaaaatga
    12481 acctgtttgg aaagacgaaa ttcggattgc gatcgatcaa acaatgaaaa acaaaaaaac
    12541 cagaacctat gaccaatttt gtaattccct caaaacattg gggattcatt gcttcaatcg
    12601 cgggaaaaat ttcacttacg aattgatcca caaaaagaaa aaagtacgat ccaacagact
    12661 aggaaaggac tacgaaaagg agacgatttt acatgagctg gacagacgag aacgaaccta
    12721 taaccaacag cgaaccaacg agctacgaag agctcgagct agcattcgcg aaactgcaga
    12781 agaagtacga agagaacaac gaactcttac gcctgaacaa ccggaaatta gaccggctcg
    12841 aagaaaccat gaacaacacg ttgaaagagg aacagaccac caacaagaat tatcgctcta
    12901 aaacgttaaa agacatagaa aacgaaataa agaaacaacg tgcttttctc gttccttata
    12961 cgcaagaaag gatacaaact acgattgatt tacaagttaa aaaactccat caagagctat
    13021 tagaccagcg aaataaggaa cagaaaaagg tgcttattca acgatcattg gattttcttg
    13081 cccccttatg ttgcttagtc ctgacactgt tcgcattttg gttagtatgg ggaggctaaa
    13141 a
//
