LOCUS       NZ_CP018740             4052 bp    DNA     linear   CON 05-MAR-2024
DEFINITION  Klebsiella pneumoniae strain Kp_Goe_121641 plasmid pKp_Goe_641-4.
ACCESSION   NZ_CP018740
VERSION     NZ_CP018740.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05412452
            Assembly: GCF_001913175.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Bohne,W., Bunk,B., Overmann,J., Gross,U. and Zautner,A.E.
  TITLE     A nosocomial outbreak of oxa48-expressing Klebsialla pneumoniae in
            Lower Saxony
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Bohne,W., Bunk,B., Overmann,J., Gross,U. and Zautner,A.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2016) Department for Medical Microbiology,
            University Medical Center Goettingen, Kreuzbergring 57, Goettingen,
            Niedersachsen 37075, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: SMRT Analysis Software v. 2.3.0
            Genome Coverage                   :: 308.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/04/2024 14:58:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,592
            CDSs (total)                      :: 5,471
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 55 of 138
            Pseudo Genes (incomplete)         :: 78 of 138
            Pseudo Genes (internal stop)      :: 24 of 138
            Pseudo Genes (multiple problems)  :: 15 of 138
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018740.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Kp_Goe_121641"
                     /isolation_source="urine sample"
                     /host="Homo sapiens"
                     /culture_collection="DSM:103707"
                     /db_xref="taxon:573"
                     /plasmid="pKp_Goe_641-4"
                     /geo_loc_name="Germany: Goettingen"
                     /lat_lon="51.53919016 N 9.91156249 E"
                     /collection_date="2013-03-12"
                     /collected_by="Andreas Erich Zautner"
                     /genotype="MLST ST101"
     gene            75..416
                     /locus_tag="BB745_RS29550"
                     /old_locus_tag="BB745_05524"
     CDS             75..416
                     /locus_tag="BB745_RS29550"
                     /old_locus_tag="BB745_05524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_057172823.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQSCLDEKPAR
                     ACRLPSISPALLRQLAGMGNNLNQIARKVNAVGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGGDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            625..>1056
                     /locus_tag="BB745_RS31560"
                     /pseudo=""
     CDS             625..>1056
                     /locus_tag="BB745_RS31560"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1094..1579
                     /locus_tag="BB745_RS30600"
                     /old_locus_tag="BB745_05525"
     CDS             1094..1579
                     /locus_tag="BB745_RS30600"
                     /old_locus_tag="BB745_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_055314654.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIAMSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            1583..1795
                     /locus_tag="BB745_RS30605"
                     /old_locus_tag="BB745_05526"
     CDS             1583..1795
                     /locus_tag="BB745_RS30605"
                     /old_locus_tag="BB745_05526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2303..2761)
                     /locus_tag="BB745_RS29560"
     CDS             complement(2303..2761)
                     /locus_tag="BB745_RS29560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016245712.1"
                     /translation="MIETTLLAVLTGALSNLTSDAIKGIIGKLASKHPEVEQQASLLET
                     PLPFDEVDKLFSQMQGAIQAAAANGEIQLDGAELKALSGIKFDHQAGTVLINGSQVQAS
                     ILVTGGGQHATGKTIIQGDTTMTSQGTSIKMSGGAQIVMTGGARIIQN"
                     /name="hypothetical protein"
     Typing          3861..4040
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.407"
                     /coverage="83.0"
                     /evalue="1.57e-69"
     Typing          complement(3398..3878)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.501"
                     /coverage="92.0"
                     /evalue="5.96e-162"
     Typing          407..1911
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.223"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgcctgcggc ttgtccaaca ccgtgccgac cgggaaacat ttcccgcccg tcccggtgtt
       61 atcaggaggc tactctggca gacaaacgca acaagatgct cacgatgtgg gtgacggaag
      121 acgaacaccg gcggctgctc gagcggtgcg acggcaggca gctggcggcg tggatgcgtc
      181 agtcctgcct cgatgagaag ccggcgcgtg cctgcagact gccgtcaata tcgccggcgc
      241 tgctgcgcca gcttgccggt atgggtaaca acctgaacca gatcgcccgg aaagtgaatg
      301 ccgttggtgc cggtcatgat cgggtgcaga ttgtggccgc cctgatggca atagacgcgg
      361 gactggagcg gctgcggcat gcggtgctgg aaaaaggggg ggatgatgat cgttaaattt
      421 catccccggg ggcgcggtgg tggtgccggt ccggtggatt acctgctggg aaaagaccgg
      481 cagcgcgagg gggcgaccgt gctgcaggga aaaccggagg aagtccggga gcttatcgat
      541 gcctcgccgt atgcgaaaaa gtacacctcc ggcgtgctgt cgttcgcgga ggctgaactg
      601 ctgcccgggc agcgggagca gatcatggcc agctttgagc gggttctgat gcccgggctc
      661 gataaagacc agtacagcat tctgtgggtg gagcacaccg acaaggggcg gctggagctg
      721 aatttcctga tccccaacac tgaactgctg accgggaaac gcctgcagcc gtattacgat
      781 cgcgccgacc ggccgcgcat cgatgcctgg cagaccgtgg tgaacggcag actgggactg
      841 catgatccaa acgccccgga gaaccggcgg ttactggtta cgccgtccgc actgccggaa
      901 acgaagctgg aagccgctca ggcgattaca cgtgggttgc tggccttagc ctcatccggg
      961 gagctcaaaa ctcgcgagga cgtcacaggt gcgctcacag cggcaggttt tgaggtggtc
     1021 cgcactacaa aaaacagtat cagcattgcc gacccggacg gggggcgaaa catccgactg
     1081 aagggagcca tttatgagca gtcttttagc gctggcgaag gacttggagc agcaatcgaa
     1141 agcgcagcag cagagtaccg gcgagatgct gaaagccgca ttcagcgagc acgagaaatc
     1201 tgtaaaagcg gaactgagcg caagcgcgaa gagaatcagc gacgccatca gcgcccacga
     1261 gcagggtatg accgcggcga tgcagtcgaa ccgcctgagc gtgctgcgta tggtcggccg
     1321 gacgtggctg accatcaccc tggtctcggt actgctgatc gccatgagcg ggagcattct
     1381 gtggtggcag gggcagcaga tcaccgggaa ttaccagact atccgggcgc aggagcggac
     1441 gcaggccatg ttgtcggaga aaaaccacgg cgttcagctg tcgctctgcg gggagaagaa
     1501 actgtcctgc gtcaaagtga acccgaaagc gggagcttac ggggaggaag ggaactggat
     1561 ggtactggag aggaaatagc ccatgacgga actggaaaaa cagttgctga ccgcattaga
     1621 gcagctacat caggactact cgcaaaggct ggacgagtgg gagagcgcct tcgcggaatg
     1681 gcagaagatg tctggtctta tgcaacggga gaacgcggcg ctgaacgagc gcgtcacgcg
     1741 cttgagtcag caggtggcga acttgagcag gcagttacag cgtttgagcc agtagtgcag
     1801 cggcatgaaa tggcggtggc cgccgaacgg gcatacgagc agcgacagca cgaaaaagag
     1861 ctggcggaag cccgttcccg tcagcggagc tataacggtc cgacgctggg ctgacaggct
     1921 tcaccagtga caaaccccac gaagaagggc tcataaattt cccgctaaat acggtcgtga
     1981 tgaaaacatc acggcgtata caacggaggg attatgagac tgtttaacgt gattgagacg
     2041 gtgctgaact gcctgtacat ggcgctggag atctgggact ggctggagaa gcattttcct
     2101 gtttttaagg cgttcatcca gattctggcc tgaaatcaga ccgtatgcat ctggctgcat
     2161 gaccatgcag ccgggtaaaa tttatctgtc acgaacgggg cggtgtatcg ggtggtttgt
     2221 tgccggtttt acctgactgc cgccccgttt gtgacgaaca tgtccggagc ggtgcgggca
     2281 agagatgtta tggtaaatca tattagttct gaatgattcg ggctccacca gtcattacta
     2341 tttgagcacc acctgacatt tttatacttg taccctggct agtcattgtc gtatcacctt
     2401 gtatgatcgt ttttccagta gcatgttgac ctccaccagt gactaaaatt gacgcttgta
     2461 cttgagatcc atttatcaaa acagttccgg cttggtgatc aaattttatt ccgctcaatg
     2521 cttttaattc agctccatct agctgaattt caccattggc tgctgcagcc tgaatggccc
     2581 cctgcatctg agaaaataat ttatcaactt cgtcaaaagg taatggtgtt tcaagcagtg
     2641 aggcttgttg ttcaacttca ggatgtttac ttgctaactt ccctatgatg cctttgattg
     2701 catcagaagt aagattgctt aatgcgccag tgagaacggc aagtagtgtg gtttcaatca
     2761 ttatatttcc ctcgttagat tgctgtgatg cgtattattt actctttata atctccgatt
     2821 ctgtccatat ggttagttgc actattacgt ttataagtgc cacttttacc atatatatat
     2881 ccaacgctac attgtggaca ttttggcgtc tcgtcattat gagtgaaatt tatttctcct
     2941 ccgacagata gggtttttac gttttccgga taatctaatg tagccacgcc ggtccattca
     3001 caattcgagc agacaacatc gaaagatgtt gggatactct tgcagttgat gttgatttgc
     3061 atatgcataa cctcgatttt gcacagaaat tacagtgtta ttagttgcat accgactata
     3121 acctgagttt gtgatggagt ggtgtttttt tgctttgact tgagtaagag aaagagatga
     3181 tcttcttgag ataatctctt tctgagcgta gtcttttgct ctgtaaacga aaaaaccacc
     3241 ctggcaggtg gtttttcgaa ggttagttaa tcctggcaga tttactaacc gtggtaacag
     3301 tcttgtgcga gacatgtcac caaagctgtc cttccagtgt agccgcagta aggccaccac
     3361 ttcaagaact ctgaatacat ctctcgcaca tcctgtttac cagtggctac tgccaatggc
     3421 gatttgtcgt gccttttcgg gttggactca agacgatagt taccgaaaag agcgtggtag
     3481 tcggactgaa cggggggatc gtgcatacag tccagcttgg agcgaactgc ctacccggaa
     3541 ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa
     3601 ccgaatggca ggaacaggag agcgcacgag ggagctacca gggggaaacg cctgggatct
     3661 ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagattttat gatactcgtc
     3721 aggggggcgg agcctatgga aaaacggcaa cgccgcatcc tcttcccggc ttctcctgat
     3781 ttgagcgctg cttgcgtatc ccgtcatctg tgagctggta acgcccgccg cagtctcacg
     3841 gcaggagcgc agcgactgag tgagcgagga ggcgtcattt cacctttacc agcatgttgc
     3901 acttacgccc gtcagccaca ttttcccctt cccacatgaa gcactttcat acattaccac
     3961 tccgccgtaa catatttagc cagtatacac tccgctagcg ctacgtgact ggttcagggc
     4021 tgcgccccga aacccgctaa aagccactga cg
//
