LOCUS       NZ_CP018739             3541 bp    DNA     linear   CON 05-MAR-2024
DEFINITION  Klebsiella pneumoniae strain Kp_Goe_121641 plasmid pKp_Goe_641-5.
ACCESSION   NZ_CP018739
VERSION     NZ_CP018739.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05412452
            Assembly: GCF_001913175.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Bohne,W., Bunk,B., Overmann,J., Gross,U. and Zautner,A.E.
  TITLE     A nosocomial outbreak of oxa48-expressing Klebsialla pneumoniae in
            Lower Saxony
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Bohne,W., Bunk,B., Overmann,J., Gross,U. and Zautner,A.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2016) Department for Medical Microbiology,
            University Medical Center Goettingen, Kreuzbergring 57, Goettingen,
            Niedersachsen 37075, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: SMRT Analysis Software v. 2.3.0
            Genome Coverage                   :: 308.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/04/2024 14:58:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,592
            CDSs (total)                      :: 5,471
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 55 of 138
            Pseudo Genes (incomplete)         :: 78 of 138
            Pseudo Genes (internal stop)      :: 24 of 138
            Pseudo Genes (multiple problems)  :: 15 of 138
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018739.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Kp_Goe_121641"
                     /isolation_source="urine sample"
                     /host="Homo sapiens"
                     /culture_collection="DSM:103707"
                     /db_xref="taxon:573"
                     /plasmid="pKp_Goe_641-5"
                     /geo_loc_name="Germany: Goettingen"
                     /lat_lon="51.53919016 N 9.91156249 E"
                     /collection_date="2013-03-12"
                     /collected_by="Andreas Erich Zautner"
                     /genotype="MLST ST101"
     gene            <1..669
                     /locus_tag="BB745_RS29520"
                     /old_locus_tag="BB745_05519"
     CDS             <1..669
                     /locus_tag="BB745_RS29520"
                     /old_locus_tag="BB745_05519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="WP_074513115.1"
                     /translation="TVGRIIMDVSIMYEASKLEYIDYKYIIKIQPDDRTLRIAVRVVFK
                     DAVTGNLITFFLSHWPSIRSADEKIREDAAESLRNNVNKILDDHGIESQIICMGDYNTN
                     PYSVAINEKLYATRDYHRIKNKRKLLFNPFWYLLSDKKTNNLGTYHYKSGASNRWFVFD
                     QMLFSSSFIYGGKDCLKLDMNSLDFHRILSDDNQCLDQRFFNTFDHYPIFCRIYHDNQK
                     "
                     /name="endonuclease"
     gene            653..1090
                     /locus_tag="BB745_RS29525"
                     /old_locus_tag="BB745_05520"
     CDS             653..1090
                     /locus_tag="BB745_RS29525"
                     /old_locus_tag="BB745_05520"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032419556.1"
                     /translation="MTTKNEEFQERLKASLKKANETLILKEKIESDISSILSMLKGITS
                     DSIDFDIFQNSSKIPKYKDCKKIVRIKKNSSMAYPKGFILFGFSINESTGYPVQVETED
                     EAAECTDVDNLKESIVYIIEKRSIEIMKLISEQQEDDDIPF"
                     /name="hypothetical protein"
     gene            complement(1900..2301)
                     /locus_tag="BB745_RS29530"
                     /old_locus_tag="BB745_05521"
     CDS             complement(1900..2301)
                     /locus_tag="BB745_RS29530"
                     /old_locus_tag="BB745_05521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2301..2492)
                     /locus_tag="BB745_RS29535"
                     /old_locus_tag="BB745_05522"
     CDS             complement(2301..2492)
                     /locus_tag="BB745_RS29535"
                     /old_locus_tag="BB745_05522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2906..3019
                     /locus_tag="BB745_RS31520"
     CDS             2906..3019
                     /locus_tag="BB745_RS31520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072032591.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPKRHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            3317..>3541
                     /locus_tag="BB745_RS30590"
                     /pseudo=""
     CDS             3317..>3541
                     /locus_tag="BB745_RS30590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422436.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /name="endonuclease/exonuclease/phosphatase family protein"
     Typing          2614..3019
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.643"
                     /coverage="99.0"
                     /evalue="4.5799999999999997e-172"
ORIGIN
        1 actgttggtc ggataattat ggatgtatcc ataatgtacg aagcatcaaa acttgagtac
       61 atagattata aatatataat taaaatacaa ccagatgata gaactttgcg tattgctgtc
      121 agagttgttt ttaaggatgc agtaacagga aatctaataa cattcttttt atcacactgg
      181 ccatccataa gaagcgcaga tgaaaagatt cgagaggacg ctgccgagtc actgcgcaac
      241 aatgtgaata aaatactaga tgaccacggc attgagtccc aaataatatg catgggtgat
      301 tacaacacaa acccttactc tgttgcaata aacgaaaaac tttacgccac tagagattac
      361 catcgaataa agaataaacg aaaacttctt tttaaccctt tttggtattt actttcagac
      421 aaaaagacca ataacttagg aacttatcat tataaatcag gagcatcgaa tagatggttt
      481 gtttttgatc aaatgttgtt ttcatcatca tttatatacg gaggaaaaga ttgtcttaag
      541 ctagacatga acagccttga ttttcatcgt atacttagtg atgataacca atgtttagat
      601 caaagatttt ttaacacttt tgatcattac ccaatatttt gtaggattta ccatgacaac
      661 caaaaatgaa gagttccaag aaagactaaa ggcatcacta aaaaaagcca atgaaacact
      721 aattttaaaa gaaaaaatag agtcagatat ttcatcaatt ttgtcaatgc taaagggtat
      781 aacatccgac tcaattgact ttgatatttt tcagaatagt tcgaaaattc ccaaatataa
      841 agattgtaaa aaaattgtca gaataaagaa aaactctagc atggcatacc ctaaaggttt
      901 cattttattt ggatttagca taaatgagtc taccggatat cctgtacagg tagaaacgga
      961 agacgaagcc gcagagtgta cagatgtgga taacttaaaa gaatcaattg tttacataat
     1021 agaaaaaaga tctatagaga taatgaaatt aatatccgag caacaagaag acgatgacat
     1081 tcctttttaa aaaggtagca tgtaatgcca ttatcaacgg cactttttca tagaaggtgc
     1141 catatatctt agaatcaact aatttaacat aagaccataa ttgtacgcac cgctaaaacg
     1201 gcctcagggc gtttctgacg ggaaaccggt aaaacaggca acaaaccacc cgaaaacccg
     1261 ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcacgcaa gtgcatagcg
     1321 cgaaaattca cgccattcag ccagggtaag tcctgtttcc ggcgttcttc ccatccgaaa
     1381 acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg cagccggaaa
     1441 accggttttc agcacgttca ggaatcgggt tcgtcgacgg ccggcaatca cgttatggca
     1501 agtcgccgga cgactcacca cacgattcac cggcgactct tcactagagt cgtacccaga
     1561 gtcgccgcgt ctaaaaaaca accaaacggg gagtcgtgtc cagagtcggg tcgttattaa
     1621 ctcattgttt ttatagtcct gagtcgttac cgactctatg acgactcgcc ggggactcta
     1681 tgacgagtcg tcatagagtc ggatctatgt aaataaaatt taaaaaacga aatttcagga
     1741 aagtaaacaa ctgatattaa atacaaatct gcaaaatttt aattttgcgt agtgtgtggg
     1801 tatttttagt cgtgaggagc gttcagagat ctggcgattt ttgggaaggt tcaggaactc
     1861 cccgggatgg ccggagagga aaactcaagc atggcaggat caggattttt tccagaattt
     1921 ccaccatggt gccggggatt ccgttaattt tgcctggtga ttttcaagaa ggagcatggc
     1981 ctgtttcagg ctgtcaatgt gcgaatcctt ggacaaaagt aaggtattca ggtgttcaat
     2041 ctcctgtttc agatgacgaa tcacctgttc actctggtca gcagaggctg tatcacgctg
     2101 ttcagagacc tgtccagaat ggtgttcacc cttcggttgt tcaaagggac caaagacacg
     2161 taaaagctct gaagtatcta ccatttttgc atttcgttca ccgctacagg tggacaggtc
     2221 acctgcttta attaacctgt gcagtgttgt tctactctta cccgtcagtc gggcagcctc
     2281 actaatagac actctggcca tcagttacca tcctgtaatg catccagtct cgaggacagt
     2341 gtacggaaaa gatgctccgc atcatcgtgg agtttctcac aaaggtcggc ctcgacatca
     2401 agatcaagct cgttaagctt ctccagcagg agcagagact gattctggat gaatttagcc
     2461 atgttcagcg ctgttttctg ttgtttcgtc ataagaagcc tcagagcgtc agtgctcagt
     2521 gtaagaacag tctcttttcc gcttcctcgc tcactcagtc gctacgctcc ggtcgttcgg
     2581 ctgcggcgag cggcaggagc atgcgtataa aagctcgaga agaccgagcg gtcaaatggg
     2641 gaagaaggaa aggccgcaaa gcggccgttt ttccataggc tccgcccccc tgacaagcat
     2701 caccgaagcg gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag
     2761 gcgtttcccc ctgatggctc cctcgtgcgc tctcctgttc ctgccattcg gtttaccggt
     2821 gtcattccgc tgttatggcc gcgtttttct cattccacgc ctgacactca gttccgggta
     2881 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg actgctgcgc
     2941 cttatccggt aactatcatc ttgagtccaa cccgaaaaga cacgacaaag cgccactggc
     3001 agcagccact ggtaactgat ttagaggagt tagtcttgaa gttatgggtc agttaaggct
     3061 acactgaaag aacaagtttt ggtgactgcg ctcctctact agccagttac ctaggttcaa
     3121 aagacctgca agttatttga acccttgaaa aaaccgccct gcaaggcggt tttttcattt
     3181 ccagccaaga agataacgcc ttaaaagggg tagcgcaaca ccatttatca acaaacagat
     3241 aaataaaatg tttaaccccc acatcaaaag caatacaata aacccaatga atacaatgca
     3301 ttaagaaaga aacaccatgg aattgaattt atgttggtgg aatattggaa tatcaccacc
     3361 tgtaaaaggc gtgaagaaag acaaggctga ggctattaga ttggccaagc actatataaa
     3421 aaaaatgtct caagaaaaaa cattggattt tttgggtatc tgtgaaatat cagaagatga
     3481 ggctatagat tttaaacctt tggctgataa actacatctt gaatacttga atttatcagg
     3541 g
//
