LOCUS       NZ_CP018453             4075 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Klebsiella pneumoniae strain Kp_Goe_71070 plasmid pKp_Goe_070-3,
            complete sequence.
ACCESSION   NZ_CP018453
VERSION     NZ_CP018453.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05412456
            Assembly: GCF_001902515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Bohne,W., Bunk,B., Overmann,J., Gross,U. and Zautner,A.E.
  TITLE     A nosocomial outbreak of oxa48-expressing Klebsialla pneumoniae in
            Lower Saxony
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Bohne,W., Bunk,B., Overmann,J., Gross,U. and Zautner,A.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2016) Department for Medical Microbiology,
            University Medical Center Goettingen, Kreuzbergring 57, Goettingen,
            Niedersachsen 37075, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: SMRT Analysis Software v. 2.3.0
            Genome Coverage                   :: 123.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/04/2024 14:59:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,627
            CDSs (total)                      :: 5,507
            Genes (coding)                    :: 5,364
            CDSs (with protein)               :: 5,364
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 60 of 143
            Pseudo Genes (incomplete)         :: 78 of 143
            Pseudo Genes (internal stop)      :: 24 of 143
            Pseudo Genes (multiple problems)  :: 15 of 143
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018453.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Kp_Goe_71070"
                     /isolation_source="urine sample"
                     /host="Homo sapiens"
                     /culture_collection="DSM:103699"
                     /db_xref="taxon:573"
                     /plasmid="pKp_Goe_070-3"
                     /geo_loc_name="Germany: Goettingen"
                     /lat_lon="51.53919016 N 9.91156249 E"
                     /collection_date="2013-04-05"
                     /collected_by="Andreas Erich Zautner"
                     /genotype="MLST ST101"
     gene            1401..1859
                     /locus_tag="BB748_RS29800"
                     /old_locus_tag="BB748_29635"
     CDS             1401..1859
                     /locus_tag="BB748_RS29800"
                     /old_locus_tag="BB748_29635"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016245712.1"
                     /translation="MIETTLLAVLTGALSNLTSDAIKGIIGKLASKHPEVEQQASLLET
                     PLPFDEVDKLFSQMQGAIQAAAANGEIQLDGAELKALSGIKFDHQAGTVLINGSQVQAS
                     ILVTGGGQHATGKTIIQGDTTMTSQGTSIKMSGGAQIVMTGGARIIQN"
                     /name="hypothetical protein"
     gene            complement(2367..2579)
                     /locus_tag="BB748_RS30910"
     CDS             complement(2367..2579)
                     /locus_tag="BB748_RS30910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2583..3068)
                     /locus_tag="BB748_RS30915"
     CDS             complement(2583..3068)
                     /locus_tag="BB748_RS30915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_055314654.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIAMSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<3106..3783)
                     /locus_tag="BB748_RS31925"
                     /pseudo=""
     CDS             complement(<3106..3783)
                     /locus_tag="BB748_RS31925"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3746..4050)
                     /locus_tag="BB748_RS29810"
                     /old_locus_tag="BB748_29645"
                     /pseudo=""
     CDS             complement(3746..4050)
                     /locus_tag="BB748_RS29810"
                     /old_locus_tag="BB748_29645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(123..302)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.407"
                     /coverage="83.0"
                     /evalue="1.57e-69"
     Typing          279..765
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.235"
                     /coverage="93.0"
                     /evalue="5.96e-162"
     Typing          complement(2252..3756)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.223"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3890..4050)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.185"
                     /coverage="95.0"
                     /evalue="8.77e-40"
ORIGIN
        1 acatcggtga gcatcttgtt gcgtttgtct gccagagtag cctcctgata acaccgggac
       61 gggcgggaaa tgtttcccgg tcggcacggt gttggacaag ccgcaggcgc gtcagtggct
      121 tttagcgggt ttcggggcgc agccctgaac cagtcacgta gcgctagcgg agtgtatact
      181 ggctaaatat gttacggcgg agtggtaatg tatgaaagtg cttcatgtgg gaaggggaaa
      241 atgtggctga cgggcgtaag tgcaacatgc tggtaaaggt gaaatgacgc ctcctcgctc
      301 actcagtcgc tgcgctcctg ccgtgagact gcggcgggcg ttaccagctc acagatgacg
      361 ggatacgcaa gcagcgctca aatcaggaga agccgggaag aggatgcggc gttgccgttt
      421 ttccataggc tccgcccccc tgacgagtat cataaaatct gacgctcaaa tcagtggtgg
      481 cgaaacccga caggactata aagatcccag gcgtttcccc ctggtagctc cctcgtgcgc
      541 tctcctgttc ctgccattcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct
      601 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca
      661 cgatcccccc gttcagtccg actaccacgc tcttttcggt aactatcgtc ttgagtccaa
      721 cccgaaaagg cacgacaaat cgccattggc agtagccact ggtaaacagg atgtgcgaga
      781 gatgtattca gagttcttga agtggtggcc ttactgcggc tacactggaa ggacagcttt
      841 ggtgacatgt ctcgcacaag actgttacca cggttagtaa atctgccagg attaactaac
      901 cttcgaaaaa ccacctgcca gggtggtttt ttcgtttaca gagcaaaaga ctacgctcag
      961 aaagagatta tctcaagaag atcatctctt tctcttactc aagtcaaagc aaaaaaacac
     1021 cactccatca caaactcagg ttatagtcgg tatgcaacta ataacactgt aatttctgtg
     1081 caaaatcgag gttatgcata tgcaaatcaa catcaactgc aagagtatcc caacatcttt
     1141 cgatgttgtc tgctcgaatt gtgaatggac cggcgtggct acattagatt atccggaaaa
     1201 cgtaaaaacc ctatctgtcg gaggagaaat aaatttcact cataatgacg agacgccaaa
     1261 atgtccacaa tgtagcgttg gatatatata tggtaaaagt ggcacttata aacgtaatag
     1321 tgcaactaac catatggaca gaatcggaga ttataaagag taaataatac gcatcacagc
     1381 aatctaacga gggaaatata atgattgaaa ccacactact tgccgttctc actggcgcat
     1441 taagcaatct tacttctgat gcaatcaaag gcatcatagg gaagttagca agtaaacatc
     1501 ctgaagttga acaacaagcc tcactgcttg aaacaccatt accttttgac gaagttgata
     1561 aattattttc tcagatgcag ggggccattc aggctgcagc agccaatggt gaaattcagc
     1621 tagatggagc tgaattaaaa gcattgagcg gaataaaatt tgatcaccaa gccggaactg
     1681 ttttgataaa tggatctcaa gtacaagcgt caattttagt cactggtgga ggtcaacatg
     1741 ctactggaaa aacgatcata caaggtgata cgacaatgac tagccagggt acaagtataa
     1801 aaatgtcagg tggtgctcaa atagtaatga ctggtggagc ccgaatcatt cagaactaat
     1861 atgatttacc ataacatctc ttgcccgcac cgctccggac atgttcgtca caaacggggc
     1921 ggcagtcagg taaaaccggc aacaaaccac ccgatacacc gccccgttcg tgacagataa
     1981 attttacccg gctgcatggt catgcagcca gatgcatacg gtctgatttc aggccagaat
     2041 ctggatgaac gccttaaaaa caggaaaatg cttctccagc cagtcccaga tctccagcgc
     2101 catgtacagg cagttcagca ccgtctcaat cacgttaaac agtctcataa tccctccgtt
     2161 gtatacgccg tgatgttttc atcacgaccg tatttagcgg gaaatttatg agcccttctt
     2221 cgtggggttt gtcactggtg aagcctgtca gcccagcgtc ggaccgttat agctccgctg
     2281 acgggaacgg gcttccgcca gctctttttc gtgctgtcgc tgctcgtatg cccgttcggc
     2341 ggccaccgcc atttcatgcc gctgcactac tggctcaaac gctgtaactg cctgctcaag
     2401 ttcgccacct gctgactcaa gcgcgtgacg cgctcgttca gcgccgcgtt ctcccgttgc
     2461 ataagaccag acatcttctg ccattccgcg aaggcgctct cccactcgtc cagcctttgc
     2521 gagtagtcct gatgtagctg ctctaatgcg gtcagcaact gtttttccag ttccgtcatg
     2581 ggctatttcc tctccagtac catccagttc ccttcctccc cgtaagctcc cgctttcggg
     2641 ttcactttga cgcaggacag tttcttctcc ccgcagagcg acagctgaac gccgtggttt
     2701 ttctccgaca acatggcctg cgtccgctcc tgcgcccgga tagtctggta attcccggtg
     2761 atctgctgcc cctgccacca cagaatgctc ccgctcatgg cgatcagcag taccgagacc
     2821 agggtgatgg tcagccacgt ccggccgacc atacgcagca cgctcaggcg gttcgactgc
     2881 atcgccgcgg tcataccctg ctcgtgggcg ctgatggcgt cgctgattct cttcgcgctt
     2941 gcgctcagtt ccgcttttac agatttctcg tgctcgctga atgcggcttt cagcatctcg
     3001 ccggtactct gctgctgcgc tttcgattgc tgctccaagt ccttcgccag cgctaaaaga
     3061 ctgctcataa atggctccct tcagtcggat gtttcgcccc ccgtccgggt cggcaatgct
     3121 gatactgttt tttgtagtgc ggaccacctc aaaacctgcc gctgtgagcg cacctgtgac
     3181 gtcctcgcga gttttgagct ccccggatga ggctaaggcc agcaacccac gtgtaatcgc
     3241 ctgagcggct tccagcttcg tttccggcag tgcggacggc gtaaccagta accgccggtt
     3301 ctccggggcg tttggatcat gcagtcccag tctgccgttc accacggtct gccaggcatc
     3361 gatgcgcggc cggtcggcgc gatcgtaata cggctgcagg cgtttcccgg tcagcagttc
     3421 agtgttgggg atcaggaaat tcagctccag ccgccccttg tcggtgtgct ccacccacag
     3481 aatgctgtac tggtctttat cgagcccggg catcagaacc cgctcaaagc tggccatgat
     3541 ctgctcccgc tgcccgggca gcagttcagc ctccgcgaac gacagcacgc cggaggtgta
     3601 ctttttcgca tacggcgagg catcgataag ctcccggact tcctccggtt ttccctgcag
     3661 cacggtcgcc ccctcgcgct gccggtcttt tcccagcagg taatccaccg gaccggcacc
     3721 accaccgcgc ccccggggat gaaatttaac gatcatcatc cccccctttt tccagcaccg
     3781 catgccgcag ccgctccagt cccgcgtcta ttgccatcag ggcggccaca atctgcaccc
     3841 gatcatgacc ggcaccaacg gcattcactt tccgggcgat ctggttcagg ttgttcccat
     3901 accggcaagc tggcgcagca gcgccggcga tattgacggc agtctgcagg cacgcgccgg
     3961 cttctcatcg aggcaggact gacgcatcca cgccgccagc tgcctgccgt cgcaccgctc
     4021 gagcagccgc cggtgttcgt cttccgtcaa cctacactag gttattcgaa gctgg
//
