LOCUS       NZ_CP018444             5234 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Klebsiella pneumoniae strain Kp_Goe_822917 plasmid pKp_Goe_917-5,
            complete sequence.
ACCESSION   NZ_CP018444
VERSION     NZ_CP018444.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05412453
            Assembly: GCF_001902415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Bohne,W., Bunk,B., Overmann,J., Gross,U. and Zautner,A.E.
  TITLE     A nosocomial outbreak of oxa48-expressing Klebsialla pneumoniae in
            Lower Saxony
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Bohne,W., Bunk,B., Overmann,J., Gross,U. and Zautner,A.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2016) Department for Medical Microbiology,
            University Medical Center Goettingen, Kreuzbergring 57, Goettingen,
            Niedersachsen 37075, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: SMRT Analysis Software v. 2.3.0
            Genome Coverage                   :: 117.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/04/2024 14:52:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,540
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,303
            CDSs (with protein)               :: 5,303
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 44 of 113
            Pseudo Genes (incomplete)         :: 69 of 113
            Pseudo Genes (internal stop)      :: 15 of 113
            Pseudo Genes (multiple problems)  :: 15 of 113
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018444.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Kp_Goe_822917"
                     /isolation_source="skin swab"
                     /host="Homo sapiens"
                     /culture_collection="DSM:103702"
                     /db_xref="taxon:573"
                     /plasmid="pKp_Goe_917-5"
                     /geo_loc_name="Germany: Goettingen"
                     /lat_lon="51.53919016 N 9.91156249 E"
                     /collection_date="2013-03-12"
                     /collected_by="Andreas Erich Zautner"
                     /genotype="MLST ST11"
     gene            complement(join(4684..5234,1..259))
                     /locus_tag="BB746_RS31235"
                     /old_locus_tag="BB746_29275"
     CDS             complement(join(4684..5234,1..259))
                     /locus_tag="BB746_RS31235"
                     /old_locus_tag="BB746_29275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032446875.1"
                     /translation="MSKDKKERSKNRYHQLVEKIFFDKYVDGDLEVLFERLDINSAAKD
                     LGIELPSNVGDVIYSIRFRTPMPESILLTQPEGKEWIIELAGRSKYRFRLVTISRILPN
                     PNMVAIKIPDNTPEIITGYSLDDEQALLAKVRYNRLIDIFLGITTYSLQNHLRTTVKGI
                     GQIEIDELYVGIDKYGCHYIIPVQAKGGTDQISMVQTSQDTAWCEQKYPNVRCRAISAQ
                     FVSSDLIALFELKIDEGQVKLIEERHYKLVPVKELDSSDISNYRMSV"
                     /name="hypothetical protein"
     gene            complement(252..1502)
                     /locus_tag="BB746_RS31240"
                     /old_locus_tag="BB746_29280"
     CDS             complement(252..1502)
                     /locus_tag="BB746_RS31240"
                     /old_locus_tag="BB746_29280"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160339.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001584442.1"
                     /translation="MTRPIAVDLFAGAGGLSLGFEQAGFDIMASVEIDPIHCSAHKYNF
                     PMCKTICRSVVDVTGEYVREVSGIGEKEIDVVIGGAPCQGFSLIGKRALDDDRNKLVYH
                     YVRLVLELKPKYFVFENVKGLTVGKHKQFLVEIMDAFKDGGYDVVSDYKVLNAADYGVP
                     QDRRRLFLMGGRKGIALPSYPEPLSIRTTVGDAIGDIPDAEKYPELWGRDWVYAKFGEQ
                     SLYSSYLHGVGKDPSDFSYPRIFNKDKLTCSLLTDHSDISRGRFVETLPDTVEPISRFK
                     KLSMNGLCNTLRAGTASDRGAFTSPRPIHPIYPRVITVREAARLHSYPDWFRFHVTKWH
                     GFRQVGNSVPPMLARSVGLQIMKVLGVTPKKPTKKIKLGDESLVAISMSDAASMFGVSS
                     NVIAKRIKNSNKEVVCE"
                     /name="DNA cytosine methyltransferase"
     gene            complement(1954..2169)
                     /locus_tag="BB746_RS30610"
     CDS             complement(1954..2169)
                     /locus_tag="BB746_RS30610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2176..2673)
                     /locus_tag="BB746_RS30615"
     CDS             complement(2176..2673)
                     /locus_tag="BB746_RS30615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2714..3412)
                     /locus_tag="BB746_RS31725"
                     /pseudo=""
     CDS             complement(<2714..3412)
                     /locus_tag="BB746_RS31725"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3351..3692)
                     /locus_tag="BB746_RS29485"
                     /old_locus_tag="BB746_29290"
     CDS             complement(3351..3692)
                     /locus_tag="BB746_RS29485"
                     /old_locus_tag="BB746_29290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4333..4464
                     /locus_tag="BB746_RS31540"
                     /old_locus_tag="BB746_29300"
     CDS             4333..4464
                     /locus_tag="BB746_RS31540"
                     /old_locus_tag="BB746_29300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072007956.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPKRHAKAPLAAATGNRIHGAE"
                     /name="replication initiation protein"
     Typing          complement(3777..3952)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.449"
                     /coverage="81.0"
                     /evalue="5.74e-59"
     Typing          4077..4461
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.832"
                     /coverage="89.0"
                     /evalue="2.91e-169"
     Typing          complement(1839..3361)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.803"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3358..3671)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.04e-58"
ORIGIN
        1 cagccagctc aataatccac tccttacctt ctggctgagt caacagaata ctttctggca
       61 taggcgttct aaatctgatt gaatatataa catcaccaac attagatggg agttctatac
      121 ctaaatcttt tgcagcactg tttatatcta acctttcaaa caaaacctct aagtcaccat
      181 caacatattt atcgaaaaat atcttttcta ccaactgatg gtatctattt ttggaccttt
      241 cttttttatc cttactcaca aacaacctcc ttgttactat tttttattct cttagcaatt
      301 acatttgatg acacaccaaa catagatgca gcatcggaca tagaaatagc aaccaaactt
      361 tcatcaccga gtttgatttt cttcgttggt ttttttggcg taacaccaag aaccttcata
      421 atctgcaagc caacacttct agctagcata ggaggaacac tattaccgac ctgtctaaat
      481 ccatgccact tagtcacatg aaatctgaac caatcaggat atgaatgcag tcttgctgcc
      541 tctctgaccg tgatgaccct aggatagatc ggatgtatag gacgaggaga tgtaaaagca
      601 cctctgtcac ttgctgttcc tgcccttaaa gtattacata gcccattcat cgagagcttt
      661 ttaaacctgc tgattggttc taccgtgtca ggcaaagttt caacaaaccg ccctctagaa
      721 atgtcagagt gatctgtcag taaagaacac gttaatttgt ctttattaaa aatccttggg
      781 tagctaaaat cactaggatc ttttcctacg ccatgcaaat aggatgagta tagtgattgc
      841 tcaccaaatt tagcataaac ccaatctcta ccccatagct caggatattt ttccgcatca
      901 ggaatatccc caatagcgtc cccaaccgtt gttctaatgg ataatggctc agggtatgat
      961 ggcagagcaa ttcctttcct tccccccatt aagaaaagac gacggcgatc ttgaggaact
     1021 ccataatcag ctgcgtttag cactttataa tcgcttacaa cgtcgtaccc gccatcttta
     1081 aatgcatcca ttatctctac taaaaactgc ttatgcttac caaccgtcaa cccttttaca
     1141 ttttcaaaaa caaagtattt aggctttagc tcaagaacca gccgtacata gtgatagacc
     1201 agtttgtttc tatcatcatc aagcgcgcgt tttcctatta atgaaaagcc ttggcaggga
     1261 gcgcctccga tcactacatc aatctctttc tcaccaatac ctgaaacttc cctaacgtat
     1321 tctccggtaa catcaacaac acttctacat atcgttttgc acataggaaa gttatatttg
     1381 tgagcagagc aatgtattgg atctatttca actgatgcca ttatatcaaa gccagcttgc
     1441 tcgaaaccga gactcagccc acccgctcca gcaaataaat caacagcaat cggtctagtc
     1501 attctttaca cccacgttat ataaaatcaa catgatgcta tcagaaaaac gatcgcttta
     1561 ccgatcgttt ttctgatttc gattgaacat aacatctctt acccgcaccg cgccggaggc
     1621 actcagggcg tttctgatgg ctgacgggca aaaggtggtg gcaagtacac cgaaaaccac
     1681 cagaaacgca cctgatttat tttacgcata tgcatcactt attcacatca ctgcatagcc
     1741 ggtgatttcg gttaaaaaag actctctgtg tgctgaaatc gcagaaaatg gccatgatcc
     1801 ccgtcacgct caggccacct gagacgggcg tttatcagtc tccatactcc ctgtcaccat
     1861 gagataatgt gcgttgccgt tccggtacca gatctgctgt cttctctggt tccttttccg
     1921 ttgtttgccc gtatcgctgt tgctcaatct cattcagcct gtcgataacc ggcgcagctg
     1981 ctcgctcaaa ctctgcacct gcctgctcaa gcccgacacg cgctcgctca gcgccgcgtt
     2041 ctcccgttgc ataagaccag acatcttccg ccattcctcg aaggcgctct cccactcgtc
     2101 cagccttctc gagtagtcct gctgtagctg ctctaacgcg ctcaacaatt gtttttccag
     2161 ttccgtcatt ctgctctact cccgtaccag cttcaccgcg ttccgcgtct tgttgtccac
     2221 cgtccggccc gtttcggctt tcatcccctc cggcagcacc agaaaccgtc cgttgcggtt
     2281 ctccagatag gtcacgcccc acgtctgcat gtgcagtttc gacagcgtat ctctctgctg
     2341 cctgatttcc gccaggttcg ccgctatccg gttcccctgg taccacagca ccccgctcag
     2401 gctggcgaac agcagccctg acaccatggt gatggtcagc cacgtccgcc cgaccattcg
     2461 cagcacgctc agccggttcg actgcatcgc cgcgttcatc ccctgttcgt gggcgctgat
     2521 ggcgtcgttg attctcttcg cgcttgcgtt cagttccgat ttgacaacgt tctcgtgctt
     2581 gctgaatgcg gctttcagcc tctcttcggt aagctgctgc tgtgctttcg atttctcctc
     2641 gaagtccctc gaggccgtta aaagactgct cataaatggc tcccctcagt cggatatttc
     2701 gccccccgtc cgggtcggca atgctgatac tgttttttgt ggttctgacc acctcaaaac
     2761 cggccgctgt gagcgcccct gtgacgtcat cacgcgtttt cagctctcct gatgaggcta
     2821 acatcagcaa accccgtgta atcgtctctg cggcttcctg cttcgtttct ggcagtcctg
     2881 aaggcgtgac cagcgcccgc cggttctccg ggtcgttcgg atcatgtagc cccagcctgc
     2941 cgttgaccac tgtctgccac gcatcgatgc gcggccggtc ggcacgatcg taatacggct
     3001 ggaggcgttt ccccgtcagt aattcggtat tggggatcag aaaattcagc tcaagacgac
     3061 ccttgtctgc gtgttccacc cacagaatgc tgtactggtc tttatcgagt ccgggcatca
     3121 gaacccgctc aaagctggcc atcagttttt cacgctgacc cggcggcagg tccgcctccg
     3181 cgaatgacag caccccggag gtatattttt tcacatacgg cgacgcatca atcagctccc
     3241 ggacttcttc cggcttcccc tgcaggacgc tggcaccttc acgctgccgg tctttaccca
     3301 gaagataatc cacaggaccg gcgcccccgc cgcgaccccg gggatggaat ttaacaatca
     3361 tcgtccggcc ctttttccag tacggcatgc cgcaaccgtt ccagaccggc atcgatcgcc
     3421 atcagcgctg ccacgatctg cacccggtca tgaccggcac caccggcgtt cactttccgg
     3481 gcgatctggt tcaggttatt gcccatgccg gcaagctgac gcagcagcgc gggcgaaatc
     3541 gagggaagcc tgccggcgcg tgtcggtttc tcatcaaggc aggtctggcg catccacgcc
     3601 gccagttgtt ttccgtcgca ccgctcaagc aaccgccggt gttcgtcttc cgtcacccac
     3661 atcgtgagca tcttgttgcg tttatctgcc agaatcgcct cctgataacg ccgtgacgga
     3721 cgggaaagcc tttcccggtc ggcgcggggt tggacaagcc gcaggcgcgt cagtggggtt
     3781 tcggggcgca gccctgaacc agtcacgtag cgctagcgga gtgtatactg gcttagtatg
     3841 ttacggcgag atggagattc ggggaagtgc ttcatgtggc aagggggaaa atacggctgc
     3901 ggggcgtaag tgcaaaatgc aggtaacgat gaaatgacgc ctcctcgctc actcggtcgc
     3961 tgcgctcctg ccgtgagact gcggcgggcg ttaccagctc acgaaggacg agataacgag
     4021 atcgcgctca agtcaggaaa gtcaggaaag tcaggaaagt caggaaagtc aggaaaaagg
     4081 atgcggcgtt gccgtttttc cataggctcc gcccccctga caagcatcac aaaatctgac
     4141 gctcaaatca gtggtggcga aacccgacag gactataaag atcccaggcg tttccccctg
     4201 gtagctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     4261 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     4321 aagctggact gtatgcacga accccccgtt cagtccgact gctgcgcctt gtccggtaac
     4381 tatcgtcttg agtccaaccc gaaaagacat gcaaaagcgc cactggcagc agccactggt
     4441 aatcggattc acggagcgga gtagagttat ctacagagtt cttgaagtgg tggcctgact
     4501 acggctacac tgaaaggaca gttttggtat ctgcgctccg tgaaagccag ttaccttcgg
     4561 aagaaagagt tggtagctct aaattccggc aaacgaacca ccgttggtag cggtggtttt
     4621 tttgtttacg gcttaggaga ttacggctag aacgaaagga tctcaagaag atcctctcag
     4681 aaattataca gacatgcggt agtttgagat gtcagagcta tctaactctt ttaccggaac
     4741 cagtttgtaa tgcctttcct caatcaattt cacttgtcct tcgtctatct ttagctcaaa
     4801 cagtgcaatc aaatcgctcg aaacaaactg agcggaaata gctctacatc taacatttgg
     4861 gtatttttgt tcacaccacg ctgtatcttg cgatgtttgc accattgata tttgatctgt
     4921 cccacctttt gcttgaactg gtattatata atggcaaccg tatttatcta tacctacata
     4981 aagttcatcg atctctattt gaccaattcc cttcaccgta gttcttaaat gattctgtaa
     5041 agaatatgtg gttataccta gaaatatatc tattagccgg ttatatctca ccttcgccag
     5101 caatgcctgc tcgtcgtcta aagaataacc tgtaattatc tccggggtat tgtctggaat
     5161 ttttattgcc accatattag gatttggcaa aatcctacta atagttacta atctaaatcg
     5221 atactttgac ctac
//
