LOCUS       NZ_CP018440             4510 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Klebsiella pneumoniae strain Kp_Goe_822917 plasmid pKp_Goe_917-6,
            complete sequence.
ACCESSION   NZ_CP018440
VERSION     NZ_CP018440.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05412453
            Assembly: GCF_001902415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Bohne,W., Bunk,B., Overmann,J., Gross,U. and Zautner,A.E.
  TITLE     A nosocomial outbreak of oxa48-expressing Klebsialla pneumoniae in
            Lower Saxony
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Bohne,W., Bunk,B., Overmann,J., Gross,U. and Zautner,A.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2016) Department for Medical Microbiology,
            University Medical Center Goettingen, Kreuzbergring 57, Goettingen,
            Niedersachsen 37075, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: SMRT Analysis Software v. 2.3.0
            Genome Coverage                   :: 117.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/04/2024 14:52:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,540
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,303
            CDSs (with protein)               :: 5,303
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 44 of 113
            Pseudo Genes (incomplete)         :: 69 of 113
            Pseudo Genes (internal stop)      :: 15 of 113
            Pseudo Genes (multiple problems)  :: 15 of 113
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018440.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Kp_Goe_822917"
                     /isolation_source="skin swab"
                     /host="Homo sapiens"
                     /culture_collection="DSM:103702"
                     /db_xref="taxon:573"
                     /plasmid="pKp_Goe_917-6"
                     /geo_loc_name="Germany: Goettingen"
                     /lat_lon="51.53919016 N 9.91156249 E"
                     /collection_date="2013-03-12"
                     /collected_by="Andreas Erich Zautner"
                     /genotype="MLST ST11"
     gene            join(4422..4510,1..232)
                     /locus_tag="BB746_RS27705"
                     /old_locus_tag="BB746_27515"
     CDS             join(4422..4510,1..232)
                     /locus_tag="BB746_RS27705"
                     /old_locus_tag="BB746_27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_072198282.1"
                     /translation="MQQNHDSDDDCRIQFRDDWLYQFYKYGKANRKIPATISVSLGRKL
                     DMLNAADKIGDLRSPPGNRFENLNPPLTGYSSIRVNDKYRLIFKWGENGVEDLYLDPHD
                     YR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            436..792
                     /locus_tag="BB746_RS27710"
                     /old_locus_tag="BB746_27520"
     CDS             436..792
                     /locus_tag="BB746_RS27710"
                     /old_locus_tag="BB746_27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_032413514.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIERVTPAHEIIER
                     KHNAESLEKKHAVA"
                     /name="HigA family addiction module antitoxin"
     gene            1621..1812
                     /locus_tag="BB746_RS27720"
                     /old_locus_tag="BB746_27530"
     CDS             1621..1812
                     /locus_tag="BB746_RS27720"
                     /old_locus_tag="BB746_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032421723.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1812..2222
                     /locus_tag="BB746_RS27725"
                     /old_locus_tag="BB746_27535"
     CDS             1812..2222
                     /locus_tag="BB746_RS27725"
                     /old_locus_tag="BB746_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_060578860.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKIIKVID
                     TSELIRVFGSLDGVIDTVKSDAGSTGVNSVGLHDLQHRIALLEAENDGLKGAVKARDEH
                     IDSLRQAMQLLEHKHEPSSPPDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            3009..3455
                     /locus_tag="BB746_RS27730"
                     /old_locus_tag="BB746_27540"
     CDS             3009..3455
                     /locus_tag="BB746_RS27730"
                     /old_locus_tag="BB746_27540"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125450400.1"
                     /translation="MNDKSGFSFMKIGAGAKVTGLRMKRNVMVVDDLSHVESFVTVGER
                     GEVINLDADGNELHTPDSYREKLKIERGELQASLEKAKEEINSIDNDITSQKIKNQLSA
                     ALSQPMDAEGEWNTKRILQGVLDLSKQVGCGVLTAAITHYLGVS"
                     /name="hypothetical protein"
     gene            complement(3997..4239)
                     /locus_tag="BB746_RS27740"
                     /old_locus_tag="BB746_27550"
     CDS             complement(3997..4239)
                     /locus_tag="BB746_RS27740"
                     /old_locus_tag="BB746_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_071993219.1"
                     /translation="MALVRSGNKTKIFEADPKFNPVFDTQHKPGDTCTYEGIYKCTGCG
                     KEVTIAGGKTLPPQNHHQHSPGQGDIRWQLFVYAE"
                     /name="protein L"
     Typing          complement(1093..1502)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcgaaaaatt ccagctacga ttagtgtttc attaggtcgc aagctggata tgctgaatgc
       61 tgctgataaa attggagatt tacggtctcc cccggggaac cgatttgaaa atctcaaccc
      121 tccattgaca gggtattcat ctataagggt gaatgataag taccgcttga tatttaagtg
      181 gggtgagaac ggagtcgagg atttgtatct cgatccacat gattaccgat aggtaacaga
      241 ccggaactgt tatcttacaa aacaagtagg tgaggcattt cctcacctac gttaaaacgt
      301 catcacaaat aatgattaat aaagtaacgc gacgtaacta tatttaagat aattactaat
      361 cgatacagct ggtaacagaa gaaatactta tcttcctcat ctaacagaga gaagcgacca
      421 attcaggagt aaagcatgtc ccaagcaacc agaaaaccat cgactgtagg agatattatt
      481 ctttatgaat atctcgagcc aagtggttta aaaatcaatg acttggctga aatgttaaac
      541 gtgcatagaa attctgcaag tgctttggtt aacaataata ggaaactgtc taccgatatg
      601 gcgtttcggt tagctaaagc attcgatact accgctaagt tctggttaaa tatacagcag
      661 tctgttgatt tgtgggaggt tacgagcagc ccacgccttc aggaagaaat agaaagagtc
      721 actcccgctc atgaaattat agaaaggaag cacaatgctg aatctttgga aaaaaaacat
      781 gcggtcgcat gattttttta aaaaaattta accaaactag aagaaggcct ctgtaatagt
      841 gtgccttctt tttttgtaac cgctaaaggt catcatcatt ttcttttacg agcacttgag
      901 atccggtttc ggtacgcgta atctcttgct ctgtaaacgg aaaaaccgcc ttgcagggcg
      961 gtttttccga aggttcaaac acttgcaggt gattgaacca aggtaactgg cttggaggag
     1021 cgcagtcacc aaaacttgtt ctttcagttt agccttaaca ggcgcatgac ttcaagacta
     1081 actcctctaa atcagttacc agtggctgct gccagtggcg cttttgcatg tcttaccggg
     1141 ttggactcaa gacgatagtt accggataag acgcagcagt cggactgaac ggggggttcg
     1201 tgcatacagt ccagcttgga gcgaactgcc ttcccggaac tgagtgtctg gcgtggaatg
     1261 agataaacgc ggccataaca gcggaatgac accggtaaac cgaatggcag gaacaggaga
     1321 gcgcacgagg gagccatcag ggggaaacgc ctgatatctt tatagtcctg tcgggtttcg
     1381 ccaccactga tttgagcgtc aatttctatg atgcttgtca ggggggcgga gcctatggaa
     1441 aaacggccgc taagcggcct ttccttctgc ccatcacgcg tccgctcggt tttctcgagc
     1501 atttatacgc atagtcctac cgctcgccgc agccgaacgg caggagcgta gcgactgagt
     1561 gagcgaggaa gcggaaaaga gactggtttt acactgagca ctgacgctct gaggcttctt
     1621 atgacgaaac aacagaaaac agcgctgaac atggctaaat tcatccagaa tcagtctctg
     1681 ctcctgctgg agaagcttaa cgagcttgat cttgatgcag aggccgacct ttgtgagaaa
     1741 ctccacgatg atgcggagca tcttttccgt acactgtcct cgagactgga tgaattacag
     1801 gatggtaact gatggccaga gtgtctatta gtgaagcggc acggcttgtt aaggttagtc
     1861 gccccacaat ttacaagatg attaacagtg ggaagttaag ttacacctct gttgtaaagc
     1921 acgggaagat cattaaagtt attgatactt cagagcttat tagagttttt ggttctcttg
     1981 atggtgtcat cgatactgta aaaagtgacg caggatctac aggtgttaac agcgttggtt
     2041 tacatgattt acaacataga attgctttac tagaagctga aaacgatggt cttaaagggg
     2101 ctgttaaggc tcgagatgag catattgatt cccttcgtca ggctatgcag cttctggagc
     2161 acaagcacga accttcttca ccacccgatt ccccgtggtg gaaattctgg aaaaaatcct
     2221 gactccgtca tgcttccgtt ttcctctccg gccatcccgg ggagttcctg aaccttccca
     2281 aaaatcgcca gatctctgaa cgctcctcac cactaaaaat acccacaccc cacgcaaaat
     2341 taaaattttg aagataattc attaatatca gttagttgtg ggttattaca ttacgcagta
     2401 ttactttctg taaggtgaat ccaagtcgtc ggcgattcgt gtacgacttg ccgacgactc
     2461 gtcatagagt catcgacgac tcacttaaga ctatcagtaa cttatgacga cttgactcta
     2521 gagtcgaatc gtcattttgg tgtttttttg accttggcga ctctagagtc aagtcacgag
     2581 tcgaatcgcc ggtgaatcgt gtggtgagtc gtccggcgac ttgcataacg tgattgccgg
     2641 ccgtcgacga acccgattcc tgaacgtgct gaaaaccgtt tttccggctg cgccggcaac
     2701 gcttcacggc cacgcaataa attgcgtccc cgttcaccgt tttcagtctg gtttggtgcg
     2761 ggttttgagt tgtctggcct gaattcaggc tgaaatccgc caccagtcag tctggtgcat
     2821 aacaattcat gcccgtaaaa cgccctggag cgattttgag gcgttttcag ggggtaagac
     2881 gtaggattta atggattacg ccccagaatc gtttctagag cgtttgagcg gtgcgcgtaa
     2941 tgaggcgtta tggtaaataa ttgctcgtga aaacgttatt aacattttta tcttatgagg
     3001 tgatgtgagt gaacgataaa tcaggcttta gttttatgaa gatcggtgca ggtgcaaaag
     3061 tgactggttt aagaatgaaa aggaatgtga tggttgttga tgatctgtct cacgtagaga
     3121 gctttgttac tgttggtgag cgaggcgaag ttattaatct cgatgcggat gggaacgaac
     3181 tacacacacc agacagttat cgtgaaaagt taaaaataga acgcggtgaa ctccaggctt
     3241 ccttggagaa agccaaggag gaaatcaact caatagataa cgatataact tcgcaaaaga
     3301 ttaagaacca gttgagtgct gcactatctc aaccaatgga tgcagaaggt gagtggaaca
     3361 ctaagagaat cctacaaggg gttttagatt tatctaaaca agtgggctgt ggtgtactca
     3421 cggctgcgat cacgcattat cttggagtta gttaataaac tacctatgcc cccatggtgg
     3481 gtatacaaaa accatgggga gcggagcgac cttcgtctag ctcttttctt gtgtgtctca
     3541 tcttgcaacg gattcgattt gtgtggggtt agtgtcagac ttcttggggt cagtttggat
     3601 atagagaatt attgtacggt aagcctgtgt tttgaacagg cttacctctc gcaatgatgt
     3661 actcccgcgc tatacgccgt ttcccgtgaa ttttccttaa tcggataacc taaatgcgat
     3721 acagaaagag cggtgactta aaagcgatcg tagctgaggt aaaatcaatc ccttacgcat
     3781 caatattggt taaataatgc gcttaacgta caaaagtttc cgatatccaa actgacccct
     3841 atttggcggc ttttcttatt ttcttgattt tcccgcatct gaacgtttct ctcggactct
     3901 tccattatta ttacgatctc gtggctgagt tccgcctcct ggtttaggga cgacgacatg
     3961 cgggttaagt ggtctacggt ctgccatttt catacctcac tctgcataaa caaatagttg
     4021 ccagcggatg tccccctgtc cgggactatg ttgatgatga ttttgtggtg gaagtgtttt
     4081 ccctcctgcg atagttactt ctttaccgca acctgtgcat ttgtaaattc cttcatacgt
     4141 gcacgtatcg ccaggcttgt gttgtgtatc aaaaactggg ttaaatttcg gatctgcttc
     4201 aaaaatcttg gttttatttc ctgagcgaac aagcgccata tgacctccaa ctattctttt
     4261 tttgcacagt agcactgtgc ttttgtgcat tttatatcat tatggttttt tgcacaagtg
     4321 cacagtgcta ttgtgcattt ctggcgtttg ttgtactatg gtggtggtaa aaggtgatct
     4381 agaacttgcc aagttgcggc aacatttgag gtaaaagaaa aatgcagcaa aatcatgata
     4441 gtgatgatga ttgccgcatt cagttccgag acgattggct ataccagttt tataaatacg
     4501 gtaaagccaa
//
