LOCUS       NZ_CP018208             4074 bp    DNA     circular CON 12-JUN-2024
DEFINITION  Escherichia coli strain MRSN346647 plasmid pMRSN346647_4.1, complete
            sequence.
ACCESSION   NZ_CP018208
VERSION     NZ_CP018208.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06067968
            Assembly: GCF_001890365.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Snesrud,E., Ong,A.C., Corey,B., Kwak,Y.I., Clifford,R., Gleeson,T.,
            Wood,S., Whitman,T.J., Lesho,E.P., Hinkle,M. and Mc Gann,P.
  TITLE     Analysis of Serial Isolates of mcr-1 positive Escheriichia coli
            Reveals a Highly Active ISApl1 Transposon
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Snesrud,E., Ong,A.C., Corey,B., Kwak,Y.I., Clifford,R., Gleeson,T.,
            Wood,S., Whitman,T.J., Lesho,E.P., Hinkle,M. and Mc Gann,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2016) Multidrug Resistant Organism Repository and
            Surveillance Network, Walter Reed Army Institute of Research, 503
            Robert Grant Ave., Silver Spring, MD 20910, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001890365.1-RS_2024_06_12
            Annotation Date                   :: 06/12/2024 04:04:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,981
            CDSs (total)                      :: 4,860
            Genes (coding)                    :: 4,685
            CDSs (with protein)               :: 4,685
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 64 of 175
            Pseudo Genes (incomplete)         :: 121 of 175
            Pseudo Genes (internal stop)      :: 38 of 175
            Pseudo Genes (multiple problems)  :: 40 of 175
            Pseudo Genes (short protein)      :: 1 of 175
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018208.1.
            Bacteria and source DNA available from
            http://wrair-www.army.mil/OtherServices_MRSN.aspx.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MRSN346647"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMRSN346647_4.1"
                     /geo_loc_name="USA"
                     /collection_date="2016-07"
                     /collected_by="MRSN"
     gene            299..991
                     /locus_tag="BSZ13_RS26875"
                     /old_locus_tag="BSZ13_26655"
     CDS             299..991
                     /locus_tag="BSZ13_RS26875"
                     /old_locus_tag="BSZ13_26655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1249..3408)
                     /gene="mobQ"
                     /locus_tag="BSZ13_RS26880"
                     /old_locus_tag="BSZ13_26660"
     CDS             complement(1249..3408)
                     /gene="mobQ"
                     /locus_tag="BSZ13_RS26880"
                     /old_locus_tag="BSZ13_26660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_016245161.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            3587..3841
                     /gene="mobC"
                     /locus_tag="BSZ13_RS26885"
                     /old_locus_tag="BSZ13_26665"
     CDS             3587..3841
                     /gene="mobC"
                     /locus_tag="BSZ13_RS26885"
                     /old_locus_tag="BSZ13_26665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091748.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKTEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(3051..3409)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.778"
                     /coverage="100.0"
                     /evalue="1.83e-175"
     Typing          3410..3586
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          300..991
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1253..3495)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.332"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccccaagtta tccacaggct cgccgttaag tttttagatc ttgtttttaa gatcttgttt
       61 ttaatacttg ttttaggggt gaaattcatt aataaaatca atatgttata gtggtatatg
      121 tggaggaatt taaggtaaag gtggaggaat ttaaggtgtt taggtggagg aatttaaggt
      181 aaaggtggag gaatttaagg tgttattcac agaaatgtga ggatataaaa aaaaacattc
      241 cacatttcct atttgtgggg cacattaaag catcaccaca tttgaacggg atctttgtat
      301 gtcggacttg attgcctaca agtcgaatgc tttggttgag gccagctaca agttgaccct
      361 acaggaacaa cgttttctgt tgctgtgtat cagtcgattg aaatctggtg ctgatgctga
      421 attgcaaaag acaatgacca ttaccgctgc ggaatatttt gattcattcc ctgacatggg
      481 gcggaaaaat gccgaggttc agttacagga agcgatcgat cgtttatggg acaggtcgat
      541 cattctcaag aatgacgaga agcgtgagga gttccgctgg atccagtatc gagcacagta
      601 cgctaaaggt gaggccaagg ctcgaataac cttttctgac gcggtaatgc cttatctgac
      661 gcaactgaag gggcaattca cgcgcgttgt tataaaaaac atatcaggtt tatcaagtag
      721 ttacagtata agaatatacg agttcctcca gcaattccgc agtacgggag agagaattat
      781 tgctcttaat gactttcgtt caatgttagg gattgaaaat aaatacaaac aattcagaga
      841 cttaaataaa atactaataa aaccctgtgt tgacgaactg aacaaaaaaa gcgacctggc
      901 tgtaacggtt gagacgatca aaaaggggcg cacggttgta gccctgcatt ttcgcttcaa
      961 ggaagacaaa cagatcaaga tgacgatcta aaatcgattt taaggcacct aaaacgctcc
     1021 ctgctgcgtt ttttttgtgt tagtagtgcg aaggtactat tgaagtgaaa aattgcgtca
     1081 aaaacgctct gatgcagcga aaaaagaacc cccaattttt gggggcggaa aaacaaaact
     1141 atgcttgcgt tatgtgagtg tgcttcttgc attcatccct gtcgatgaac attgaagaac
     1201 atcgacgaac ataacacgat gttaaacaaa acaaaatatt gttcacattc actgtgacca
     1261 accgcgatca tgtctcctct cggtggtgtg tgttcttgca tcttcctcgc gccgccattc
     1321 gtcccaggta cgattgaaac ccagcgagcg gaacagatga tccggctgtt tttctggtgt
     1381 gttgacggca gcaaaaagcg gtgcctcacg ggttttcaga tgaaactcca taagttccgg
     1441 cgcaggtgat atgtggctgt ctgcatcgtg atttacggca acaccatacc ctgcataccg
     1501 taacccctta tcggtcagtg cactgatgac cagctggctg ttatcggctg agggaacggc
     1561 aaacgcggga tacagcgtac cattaagccg gacaacaccg cccagagagt catccacgcc
     1621 catgaccagc aaaagctgaa gctggcgggt ttcccggtcg acagaactca ttaccggcat
     1681 cagcgtcggt ggcagacgtc cctgcgtaaa atccaccttc cttgctgcca gtgatggcat
     1741 ggctggctgg cggtcttctc caagacagaa agactccggc catgtcttcg gctggcggag
     1801 attgcgggta tattgcggcc atgtctggca aacctgcgtc agaagttgct caaaggtcgc
     1861 agaggaataa acacgttgca gtggtggatg ttgaccgatt tcgcttcggt cacagatata
     1921 accactcctg tcacgcatta aatcaacacg ctcaaagatt ctggtgcggt atttctccag
     1981 ctcgttcccc cagagcgtat attctccgtt ccggttgtga agcagaaggt aacaggtcat
     2041 ttccccgtta ccaggcatgg gggcctcgcc ataccccgta acgactgcgc tcagtacccc
     2101 tgaatccata acggcattgt catttttcct gagctgttcc gctcgtcgct ccgcgcgact
     2161 acgttctgta tcctcctgct ccttccggcg caattcctct tcacgtttaa gcaactggac
     2221 tctgagagct gcaaggccgg atgcccccca ctgctgccac tgtttttcaa atgccgtctg
     2281 actcgcggga atgtcctgtt tctccacctt ccggtaatgc tgtcgcaatt tttccatttg
     2341 ctggctcagc gactggattt ttccctgttc gctacggtat tccggtgccg cccattgcat
     2401 caggggaata cgggaaaaac cggacgaaaa cggcgatgat tttttcaggc tggcagcatg
     2461 gctcatcgct tcctttcgtt cggtgttcag ctcttcccag cgtctgcgcg tcgcctgcat
     2521 atctgccatc atcgacatgg cattactggt gagtttccat gccgcattac tgtccttcgg
     2581 caacgcttcg atgatgtcac gtagtgtgta cgaagttgcg ttgtcaggaa gcacgatatt
     2641 cagaaggtca gccacacggc gggcaggctc tgtcagcccg ctgttcctga gttcagtgcg
     2701 ggaaatctgt atttccaggt taagcctgtt aatcagacga ttctgttcgc cgcgttctgt
     2761 ttcgataccg cgtgtttcca tcgcacaggc agactttccg aggtgaatac caggcgtttt
     2821 ctcaagcccc tgtcgctcgt atgaacgatg gtctatctct tcctgataac cggcgttcgc
     2881 cagtgctctg tttgcatggt cagcccatga tgcacgccac gtctcggcat gtgtccggtc
     2941 gttccagtcc ctgacctttc cgccgaaccc agcaggtccg acagccctcg tggtcagcat
     3001 gatgtgcgca tggggatttg ttttatccat gtgatggaac gccacatcag caatcatgcc
     3061 ctgactgaca aaattttctc tgacaaaggc gagaacggtt tcccgtgcgg catcctgcgg
     3121 cagttcacgg gggatagcga gttcaatttc ccgcgcaagt tgtgagttct tccggcgttc
     3181 ggccttctca acggcattcc agagttcggc acggttcgta caccaagcag gggctgtcgc
     3241 gggggtcaga atcacagctt cagccacacc gcttttgcgg gtgtagtcgt gagtcagtcc
     3301 ggtacgctca tcaagcatca gtgaaccgga acgataggca gcggaagcta cggcgctgta
     3361 gccgctgctt ctggagataa ttttcatgct gagatgataa atcgccattt ctgccgtaat
     3421 gcctttttgt agttttcttt cagccccgca gggcgcacac aatcgaaacg gagtggagat
     3481 tgtataagtg cgcacttcgt gatttgtcgt gcattatgcc agctcagttt ttaaactacc
     3541 agtggcgcgc taaaatgcga tttttcatcg cgcacggagt tacatcatga gcagaacgct
     3601 cgaacagaag attgctgatg ctgaggcgag attacagcgc ctgaaggcga agagccggag
     3661 cctggataca gcgcagaagg ttgttgtggg tgcagcattg ctggcaaagg tcagaaagcc
     3721 ggaggaggtg cagttgcgtg cctggttact ccagttcctg aagacagagg tgacacgaca
     3781 ggctgatgtg acgcgcatac tgccgctgat taacgagctg gaggcgctgc caggacagtg
     3841 attgtatcgt ccgctcgccg cagccccagc gaccgagcgt tagcgagcga gtgggcgagg
     3901 aagcggaagt gaatccgtcg ccaggttgag cacatacgca cggtttttcg gcaggtgata
     3961 tgcgctgaac ccgcatgaat actggatttc cacagggttg gagggggagt attcggaact
     4021 tgcaattctg ctttcttgca acacattgtt tttattggtt ttttgttttt agca
//
