LOCUS       NZ_CP018068            13112 bp    DNA     circular CON 31-JAN-2024
DEFINITION  Enterococcus faecium strain E1 plasmid pE1_13, complete sequence.
ACCESSION   NZ_CP018068
VERSION     NZ_CP018068.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06019998
            Assembly: GCF_001886635.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13112)
  AUTHORS   Tedim,A.P., Lanza,V.F., Manrique,M., Pareja,E., Ruiz-Garbajosa,P.,
            Canton,R., Baquero,F., Coque,T.M. and Tobes,R.
  TITLE     Complete Genome Sequence of E. faecium ST117 a globally disseminated
            multidrug resistant clone
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13112)
  AUTHORS   Tedim,A.P., Lanza,V.F., Manrique,M., Pareja,E., Ruiz-Garbajosa,P.,
            Canton,R., Baquero,F., Coque,T.M. and Tobes,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2016) Microbiology Laboratory, Instituto Ramon y
            Cajal de Investigacion Sanitaria (IRYCIS), Carretera Colmenar Viejo
            km9.1, Madrid 28034, Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 800.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 03:04:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,196
            CDSs (total)                      :: 3,103
            Genes (coding)                    :: 3,003
            CDSs (with protein)               :: 3,003
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 35 of 100
            Pseudo Genes (incomplete)         :: 65 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 19 of 100
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018068.1.
            Bacteria and source DNA available from Teresa Coque. Hospital Ramon
            y Cajal. Microbiology department.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="E1"
                     /isolation_source="Blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pE1_13"
                     /geo_loc_name="Spain: Madrid"
                     /lat_lon="40.48 N 3.69 W"
                     /collection_date="2010-01-11"
                     /collected_by="Hospital Ramon y Cajal. Microbiology
                     Department"
                     /subtype="ST117"
                     /subgroup="BAPS2.1a"
     gene            complement(264..680)
                     /locus_tag="BO233_RS16720"
                     /old_locus_tag="BO233_15695"
     CDS             complement(264..680)
                     /locus_tag="BO233_RS16720"
                     /old_locus_tag="BO233_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002368067.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(682..963)
                     /locus_tag="BO233_RS16725"
                     /old_locus_tag="BO233_15700"
     CDS             complement(682..963)
                     /locus_tag="BO233_RS16725"
                     /old_locus_tag="BO233_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002368064.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIENDF
                     DQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            complement(1259..1747)
                     /locus_tag="BO233_RS16730"
                     /old_locus_tag="BO233_15705"
     CDS             complement(1259..1747)
                     /locus_tag="BO233_RS16730"
                     /old_locus_tag="BO233_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_060763418.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKEA
                     VTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQQK
                     LLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            complement(1757..2725)
                     /locus_tag="BO233_RS16735"
                     /old_locus_tag="BO233_15710"
     CDS             complement(1757..2725)
                     /locus_tag="BO233_RS16735"
                     /old_locus_tag="BO233_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002368293.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     ADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIANFS
                     KEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELLQQLAE
                     LERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
                     /name="replication initiation protein"
     gene            complement(<3413..3558)
                     /locus_tag="BO233_RS17505"
                     /pseudo=""
     CDS             complement(<3413..3558)
                     /locus_tag="BO233_RS17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            complement(4018..4344)
                     /gene="bacA"
                     /locus_tag="BO233_RS16745"
                     /old_locus_tag="BO233_15715"
     CDS             complement(4018..4344)
                     /gene="bacA"
                     /locus_tag="BO233_RS16745"
                     /old_locus_tag="BO233_15715"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="WP_181047671.1"
                     /translation="MEAASSRYNHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQI
                     ASFIPIGGVGTWVITNAFSSAVDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYAR
                     YVIK"
                     /name="bacA"
     gene            4598..5002
                     /gene="tnpA"
                     /locus_tag="BO233_RS16750"
                     /old_locus_tag="BO233_15720"
     CDS             4598..5002
                     /gene="tnpA"
                     /locus_tag="BO233_RS16750"
                     /old_locus_tag="BO233_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            5019..6167
                     /gene="tnpB"
                     /locus_tag="BO233_RS16755"
                     /old_locus_tag="BO233_15725"
     CDS             5019..6167
                     /gene="tnpB"
                     /locus_tag="BO233_RS16755"
                     /old_locus_tag="BO233_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(6946..8094)
                     /gene="tnpB"
                     /locus_tag="BO233_RS16760"
                     /old_locus_tag="BO233_15730"
     CDS             complement(6946..8094)
                     /gene="tnpB"
                     /locus_tag="BO233_RS16760"
                     /old_locus_tag="BO233_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(8111..8515)
                     /gene="tnpA"
                     /locus_tag="BO233_RS16765"
                     /old_locus_tag="BO233_15735"
     CDS             complement(8111..8515)
                     /gene="tnpA"
                     /locus_tag="BO233_RS16765"
                     /old_locus_tag="BO233_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            complement(8848..9264)
                     /locus_tag="BO233_RS16770"
                     /old_locus_tag="BO233_15740"
     CDS             complement(8848..9264)
                     /locus_tag="BO233_RS16770"
                     /old_locus_tag="BO233_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002368067.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(9266..9547)
                     /locus_tag="BO233_RS16775"
                     /old_locus_tag="BO233_15745"
     CDS             complement(9266..9547)
                     /locus_tag="BO233_RS16775"
                     /old_locus_tag="BO233_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002368064.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIENDF
                     DQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            complement(9843..10331)
                     /locus_tag="BO233_RS16780"
                     /old_locus_tag="BO233_15750"
     CDS             complement(9843..10331)
                     /locus_tag="BO233_RS16780"
                     /old_locus_tag="BO233_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_060763418.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKEA
                     VTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQQK
                     LLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            complement(10341..11309)
                     /locus_tag="BO233_RS16785"
                     /old_locus_tag="BO233_15755"
     CDS             complement(10341..11309)
                     /locus_tag="BO233_RS16785"
                     /old_locus_tag="BO233_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002368293.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     ADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIANFS
                     KEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELLQQLAE
                     LERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
                     /name="replication initiation protein"
     gene            complement(<11997..12142)
                     /locus_tag="BO233_RS17510"
                     /pseudo=""
     CDS             complement(<11997..12142)
                     /locus_tag="BO233_RS17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            complement(12602..12928)
                     /gene="bacA"
                     /locus_tag="BO233_RS16795"
                     /old_locus_tag="BO233_15760"
     CDS             complement(12602..12928)
                     /gene="bacA"
                     /locus_tag="BO233_RS16795"
                     /old_locus_tag="BO233_15760"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="WP_181047671.1"
                     /translation="MEAASSRYNHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQI
                     ASFIPIGGVGTWVITNAFSSAVDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYAR
                     YVIK"
                     /name="bacA"
     Typing          complement(1758..2725)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(10342..11309)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gttgtcggtc ttcgagttac caacggtaac cctcataccg aacggcattc atcacccacc
       61 tatagaggat gggcgacttc tgcctaattc tgttaaataa ccgatatttg gagattgggg
      121 cagcgcccca aaatgcaaaa agaaggattc gcacaagcgg atttttcttt ttttgtgcaa
      181 gttaccggta acttgcaaat cttgctggaa gactgccccc taaaaaagaa actgagaaat
      241 agaaaaaatt ttcggtttct ttttcatctt tttagaaagt ttgttgggtc aaaatttcta
      301 gaagtgaatg tttgattggt ttctttttga cgatttttgg gttgatttcg tcgggaggga
      361 ttgagttgat tttcaatttt ttctttcact tcttgatata ctttagaatt tgtaccgata
      421 agaacaccag tatcttttag atataaatca gcaaattctg caatgatttc ggacataata
      481 gaaatttctt ttcccatcgc atttagtttt gcttggttgc tggtagttgt cgtattagta
      541 ttaggttcat aactcataca tagaaaacga atggcttccg agtagttatt gatatctgga
      601 tttttttcga tgatggaatc taaaacgtca atgtgactgg gataaagggt aacgtgtttt
      661 tgaacagaag tttttctcat gttatattcc tccgataagt gtttcaatga gttggttgtt
      721 tgtatgaaca tagtcgttca atgtttcagt attttttgct tgtatatcga taaatttttc
      781 gagtaataaa tcgaattgat cttttttgta atgcctaagt gcctggtcaa aatcgttttc
      841 gattagaact tttaggtacg cattacggct ccattttttg ccagtttttg ttgtgagttc
      901 ttggcaacgt ctatctattt ctgaaataac ttttggatca atattccgaa ctaaaatgtc
      961 catagaagaa ttcctttcgc atttgtgtat aatttatgta tatcatacaa atatagtatt
     1021 tgtatagctg ttttttgccg aaacactata cataagctat ccacaatgac gaaaaagtga
     1081 tagcaaattt ttttcttaga aacgtggtag gcacagccta cctagcacct ttcgtaagaa
     1141 agtgataccg cgtttcctta tgctctttaa aaaatagcga cgtgagatca tgtcgctatg
     1201 cttgtaaaag caaaaaaaga aagtaaccca acaattttta tgaataaaaa ttgttgggtt
     1261 attgccagaa ttgccaccat tttttctcct tgttttgtcc tttttgagtt tcttcagtaa
     1321 cagaaagggg ttctttttct gaagattctt ctgcataggt tagctggagt tgttcgtgaa
     1381 gttttttgat ttgttgattg gcttgtaggg tgagttgttg ttgctggtcg agtaattttt
     1441 gctgcgtttc taaagctatt tgaagttttt caatttgcct atctttttct tcaatatgat
     1501 tattcttagt ttcaatctcc ttgagtaggt aatcggtagg taatgaatcg ggtaattcac
     1561 taggtaagtt acccgattca ttacctgata aaacctttgt aattgttgtt actgcttctt
     1621 ttttcaggta ggtaatattt ccttttttgt aggtaaaatt actaggtaat ttcctcagtt
     1681 gatacttcac tttatctttt gaaacaccta gaatatctgc taattctttg atagttttat
     1741 ctgtcataaa aaccccttat tctttaaata agttttggag attatctagt aagttttctt
     1801 taaattctgg ggtaatatcg gaattgttat ctgctatttg tttgttaatt ttcttacttg
     1861 gggtattttg ttttctttct aattctgcta actgttgtaa aagttctaat tttcgttttt
     1921 gttctttgat tttttcttgt aatgaagtag gttctttttc agattgaacc tgttgattag
     1981 ctagttccaa agctttattt tgttcttctt tggttggagc atgtttgtta aagtcgtctg
     2041 atttttttat ctcaggttta aatgtaaatt gatagccaat gacaggcttt ccccgtcctt
     2101 ttccgtatct cttttggatt gtcaaacctt tgaagattgc ggttagttct tggcgaatag
     2161 gctttataat ttttttattt atatcacttt ctctataact tttagggata tcaagtaatt
     2221 catgaaagtc ttcttttgaa aaattggcaa ttcctttggt tctgtattgt tttaataatc
     2281 taaacatggt tttggaataa ctgctttcta attcattgaa ttgttggaga gaatatcgaa
     2341 cccattcttc aagattatta agtaagggta aggctttctc atggacttgt atttcagcaa
     2401 aaggaatatc agcttttcca tcgattttaa attgattaaa taaaacaaat cgttcacgtt
     2461 gcaaaccgtc agcacttctt ctaccaaaac gtaaatccat taatttgtca tacgttgttt
     2521 ctaaatcatc tatgaatcga acatttgcag ttgctttata attgcttaaa tcttttaatt
     2581 gttcgaatgt aaattgtact tttttatcgc ctacatcacg cattctagat attatagaga
     2641 agaataaatt catttctact gacgtaaatt tgcgtaaagg aatcgtattt aattcatggt
     2701 ggtatttcac aatttcattt gccataaaat cctccttcaa gattaactac ttaacaaaat
     2761 tataaagagc tttctaatgg gggtcaaccc cctaattaaa gacccctaaa aacagacaaa
     2821 aagaccccta aaaacagaca aaaagacccc taaaaacaga caaaaagacc cctaaaaaca
     2881 gacaaaaaga cccctaaaag attctgcaca ccttgggaga gtaaggatac agccgacggt
     2941 aaagaggttt aaaaagaggt tataaaagag attataaaag aggggggcaa aatttcccgc
     3001 ctaaaatttt cctacttttt ttctccaaac aggttcgcga aatttcctaa actgggaatc
     3061 tgctccctac ggtcgcttta aaggtgtgat cgctacgctc tcacattaaa ctgcgcagtc
     3121 gcctaaaggc tcctttgctt aaaaacagtt cgtaaggagt ccttttagaa gctctgagcg
     3181 ctcatctacg gcgttttaag acaagtctag ataaaatccc ttatcttgtt tttaaagcgc
     3241 cttacaggcg gtcagggcaa gccctaacaa ccctaaaacg ttctacagaa cctctaaaat
     3301 tcgttttaag gcgttttaaa gtcgctttgg tataattaca taccagaacg tttaaaacgc
     3361 cttaaaacgc aaattagagc cctatgtata tatcgactaa tgttacctag acttttacgg
     3421 aagccggcac actcatttgc aaagcttcag agttgcaaat ttggattcgc tcactcgaaa
     3481 actgatttat tttgactctg atcaataggt gtatttttta gaaggattgc tagccaaatt
     3541 ttgctttaat ttgctcataa gtcccaccct tttgatacgt ccataggacg tatcgtttgt
     3601 aatgtgaaag tgactataag gtccctttct acaaaacaca ctaaagggct tggcaagctc
     3661 tatatttgct agccaaatac ctccgtattt ggtgcaaata accgcttgtc aaaggggcgc
     3721 aagctccccc ttgaatcccc ctcaaaaccc acgctccaca atacaaaaag tggctagaaa
     3781 atgctagcca ctttttgtaa aatttagtat gatttatttt tagctattta gagtttttat
     3841 ttccgtaaat acttctaaaa attgctaaga taaatgctat aggaattaga taaagaatac
     3901 ttttttgtaa gcttatattg aaaattagcc taaatatgaa gaacatagtg attaaaacta
     3961 ttattgaaat aattacagct aaaatctttt tattcatgag atagtttcac tttctgatta
     4021 ttttattaca taacgagcgt aatcattgta ttgatatgat gttgtattgc taaagtgagc
     4081 attatatgtt aattgtacac cttttccttg atttgcagct cttacaaaga cattcattgc
     4141 atcaacagca ctactaaaag catttgtaat aacccatgtt ccaactccac caataggaat
     4201 aaaagatgca atttgagcag gagtctttag cttattataa ttttttttca aatctctaca
     4261 cattttatta gtaacataat agttcatttt atagcaagta aatccacgat gattatgatt
     4321 atatcgtgaa cttgcagctt ccacttttat acctctagca ctaaaaggaa acagatttaa
     4381 cggagtactt gctccaacaa tcataccgaa caaaaataga caagtaataa ttttaacaga
     4441 attaggcaga agtcgcccat cctctatagg tgggtgatga atgccgttcg gtatgagggt
     4501 taccgttggt aactcgaaga ccgacaacta aggcgtattg cctttttatt ttgttccatg
     4561 ttacaatcag tctatacaag aaatgagttg atataacatg gaattagata caaataatca
     4621 ttcagtattt cttctttatt accaccttgt tttagtaacg aaatatcgta gacaagtgat
     4681 tgatgatgaa atatctgatt atgccaaaac tacttttgaa agaatttcag aatcgtatca
     4741 cattacttta gtggagtgga atcatgataa agaccacgtt catattatgt tcaaagctca
     4801 acctaaaaca gaattgacaa aattcatcaa tgcctataaa agtgccagct caagattgat
     4861 aaaacgtgac tttccaagag tcaaacaatt tctttggaaa gaaatgttct ggtctaaaag
     4921 tttttgtctt ctgacaactg gtggcgcacc aattgatgtg attaagaagt atatccaaaa
     4981 tcaaggaaac aatcataaat agaaagtagg tgaactttat ggaacaacta aaagcataca
     5041 aattcagaat ctatccaaca gaggaacaag aaatcttctt tgccaaatct tttggttgtg
     5101 tccgtaaggt ctacaatcta atgcttgatg accgaaaaaa ggcgtatgaa gaagttaaaa
     5161 atgattcttc taaaaaaatg acttttccga caccagctaa atacaagaaa gaatttccgt
     5221 ttttgaaaga aattgatagc cttgctttag ccaatgccca acttaattta gataaagcat
     5281 acaagaattt ttttcgggat aaatccgttg gatttccacg tttcaaaagt aagaaaaatc
     5341 ctgttcagag ttatacaacg aataatcaaa atggtacagt tgctttgatt gatagtaaat
     5401 tcatcaaagt tcctaaattg aagtcattag taagaattaa gcttcataga caaccaaaag
     5461 ggattatcaa atctgctaca atatcacgtc attctagcgg taaatactat atttctttgt
     5521 tgtgtaaaga agaaattatc gaattaccta aaactaattc cacaattggt attgacttag
     5581 gtattatgga ctttgcgatt ctttctgatg gacaaaaaat tgataataat aaattcacgt
     5641 ccaaaatgga aaagaaacta aagcgtgaac aacgcaaatt gtctaggcgt gctttattgg
     5701 ctaaaaataa aggtaccaat ctatttgaag ctaaaaatta tcaaaaacaa aaacgcaaag
     5761 tagctagatt gcatgaaaaa gtaatgaatc aacgtactga ttttctgaat aagttgagta
     5821 cagaaatgat caaaaatcac gatattatct gtattgaaga cttaaacaca aaaggtatgt
     5881 tgcgaaatca taaattagct aaaagtattt ctgatgtttc ttggtcaaag tttgtgacta
     5941 aattacaata caaggctgac tggtatggac gaaaaatcat caaagtagat aaatggtttc
     6001 cgtctagtca gatttgttca gagtgcggac ataaagatgg caagaaatct cttgaaattc
     6061 gagaatggac ttgtcctgtt tgtcatactc atcatgatcg tgatatcaat gctagtatca
     6121 atattctgac cgaaggtcta agactacatt ctatgggatt ggcttagatc caatcatcaa
     6181 gaaccgtagg aactacgggg atagcttggt aaataagaga caccgctgtt agtaaagaaa
     6241 tacgctatca agtatgctct attcccaaga agctcccact tcaagcgtta agaaccgtca
     6301 ggtttagcta agtggtgagt agttcactaa ttttattttt cattgttact tcctccttag
     6361 atataaaatc ctaggaaagc gctttctgta tatatttaga ataatcagaa aattaaatat
     6421 tgtcaagagg gtttatttta taaaacgtgt tattttaaag gttagttgac ataatgtcga
     6481 gtatgccaag tagcccttgt atcaaccgat acaggggctc actttgtgat ttttcattgg
     6541 tctagctaaa aggtacgttc ggaagcttga ttttccgcta ttgtctgttt acgattcttt
     6601 gtgaatctta accgattcct ttttagaaac ttgttatttt tcataaaata tggtaaattt
     6661 ttggcagcgc ttcataagcg cgctgctttt tcaagaataa acgctcatta ttttttattg
     6721 gctgctcgtt agagcagtct ttttttattt cagagtacgt tctatttttt tgagttacca
     6781 cgctagtgaa ctactcacca cttagctaaa cctgacggtt cttaacgctt gaagtgggag
     6841 cttcttggga atagagcata cttgatagcg tatttcttta ctaacagcgg tgtctcttat
     6901 ttaccaagct atccccgtag ttcctacggt tcttgatgat tggatctaag ccaatcccat
     6961 agaatgtagt cttagacctt cggtcagaat attgatacta gcattgatat cacgatcatg
     7021 atgagtatga caaacaggac aagtccattc tcgaatttca agagatttct tgccatcttt
     7081 atgtccgcac tctgaacaaa tctgactaga cggaaaccat ttatctactt tgatgatttt
     7141 tcgtccatac cagtcagcct tgtattgtaa tttagtcaca aactttgacc aagaaacatc
     7201 agaaatactt ttagctaatt tatgatttcg caacatacct tttgtgttta agtcttcaat
     7261 acagataata tcgtgatttt tgatcatttc tgtactcaac ttattcagaa aatcagtacg
     7321 ttgattcatt actttttcat gcaatctagc tactttgcgt ttttgttttt gataattttt
     7381 agcttcaaat agattggtac ctttattttt agccaataaa gcacgcctag acaatttgcg
     7441 ttgttcacgc tttagtttct tttccatttt ggacgtgaat ttattattat caattttttg
     7501 tccatcagaa agaatcgcaa agtccataat acctaagtca ataccaattg tggaattagt
     7561 tttaggtaat tcgataattt cttctttaca caacaaagaa atatagtatt taccgctaga
     7621 atgacgtgat attgtagcag atttgataat cccttttggt tgtctatgaa gcttaattct
     7681 tactaatgac ttcaatttag gaactttgat gaatttacta tcaatcaaag caactgtacc
     7741 attttgatta ttcgttgtat aactctgaac aggatttttc ttacttttga aacgtggaaa
     7801 tccaacggat ttatcccgaa aaaaattctt gtatgcttta tctaaattaa gttgggcatt
     7861 ggctaaagca aggctatcaa tttctttcaa aaacggaaat tctttcttgt atttagctgg
     7921 tgtcggaaaa gtcatttttt tagaagaatc atttttaact tcttcatacg ccttttttcg
     7981 gtcatcaagc attagattgt agaccttacg gacacaacca aaagatttgg caaagaagat
     8041 ttcttgttcc tctgttggat agattctgaa tttgtatgct tttagttgtt ccataaagtt
     8101 cacctacttt ctatttatga ttgtttcctt gattttggat atacttctta atcacatcaa
     8161 ttggtgcgcc accagttgtc agaagacaaa aacttttaga ccagaacatt tctttccaaa
     8221 gaaattgttt gactcttgga aagtcacgtt ttatcaatct tgagctggca cttttatagg
     8281 cattgatgaa ttttgtcaat tctgttttag gttgagcttt gaacataata tgaacgtggt
     8341 ctttatcatg attccactcc actaaagtaa tgtgatacga ttctgaaatt ctttcaaaag
     8401 tagttttggc ataatcagat atttcatcat caatcacttg tctacgatat ttcgttacta
     8461 aaacaaggtg gtaataaaga agaaatactg aatgattatt tgtatctaat tccatgttat
     8521 atcaactcat ttcttgtata gactgattgt aacatggaac aaaataaaaa ggcaatacgc
     8581 cttagttgtc ggtcttcgag ttaccaacgg taaccctcat accgaacggc attcatcacc
     8641 cacctataga ggatgggcga cttctgccta attctgttaa ataaccgata tttggagatt
     8701 ggggcagcgc cccaaaatgc aaaaagaagg attcgcacaa gcggattttt ctttttttgt
     8761 gcaagttacc ggtaacttgc aaatcttgct ggaagactgc cccctaaaaa agaaactgag
     8821 aaatagaaaa aattttcggt ttctttttca tctttttaga aagtttgttg ggtcaaaatt
     8881 tctagaagtg aatgtttgat tggtttcttt ttgacgattt ttgggttgat ttcgtcggga
     8941 gggattgagt tgattttcaa ttttttcttt cacttcttga tatactttag aatttgtacc
     9001 gataagaaca ccagtatctt ttagatataa atcagcaaat tctgcaatga tttcggacat
     9061 aatagaaatt tcttttccca tcgcatttag ttttgcttgg ttgctggtag ttgtcgtatt
     9121 agtattaggt tcataactca tacatagaaa acgaatggct tccgagtagt tattgatatc
     9181 tggatttttt tcgatgatgg aatctaaaac gtcaatgtga ctgggataaa gggtaacgtg
     9241 tttttgaaca gaagtttttc tcatgttata ttcctccgat aagtgtttca atgagttggt
     9301 tgtttgtatg aacatagtcg ttcaatgttt cagtattttt tgcttgtata tcgataaatt
     9361 tttcgagtaa taaatcgaat tgatcttttt tgtaatgcct aagtgcctgg tcaaaatcgt
     9421 tttcgattag aacttttagg tacgcattac ggctccattt tttgccagtt tttgttgtga
     9481 gttcttggca acgtctatct atttctgaaa taacttttgg atcaatattc cgaactaaaa
     9541 tgtccataga agaattcctt tcgcatttgt gtataattta tgtatatcat acaaatatag
     9601 tatttgtata gctgtttttt gccgaaacac tatacataag ctatccacaa tgacgaaaaa
     9661 gtgatagcaa atttttttct tagaaacgtg gtaggcacag cctacctagc acctttcgta
     9721 agaaagtgat accgcgtttc cttatgctct ttaaaaaata gcgacgtgag atcatgtcgc
     9781 tatgcttgta aaagcaaaaa aagaaagtaa cccaacaatt tttatgaata aaaattgttg
     9841 ggttattgcc agaattgcca ccattttttc tccttgtttt gtcctttttg agtttcttca
     9901 gtaacagaaa ggggttcttt ttctgaagat tcttctgcat aggttagctg gagttgttcg
     9961 tgaagttttt tgatttgttg attggcttgt agggtgagtt gttgttgctg gtcgagtaat
    10021 ttttgctgcg tttctaaagc tatttgaagt ttttcaattt gcctatcttt ttcttcaata
    10081 tgattattct tagtttcaat ctccttgagt aggtaatcgg taggtaatga atcgggtaat
    10141 tcactaggta agttacccga ttcattacct gataaaacct ttgtaattgt tgttactgct
    10201 tcttttttca ggtaggtaat atttcctttt ttgtaggtaa aattactagg taatttcctc
    10261 agttgatact tcactttatc ttttgaaaca cctagaatat ctgctaattc tttgatagtt
    10321 ttatctgtca taaaaacccc ttattcttta aataagtttt ggagattatc tagtaagttt
    10381 tctttaaatt ctggggtaat atcggaattg ttatctgcta tttgtttgtt aattttctta
    10441 cttggggtat tttgttttct ttctaattct gctaactgtt gtaaaagttc taattttcgt
    10501 ttttgttctt tgattttttc ttgtaatgaa gtaggttctt tttcagattg aacctgttga
    10561 ttagctagtt ccaaagcttt attttgttct tctttggttg gagcatgttt gttaaagtcg
    10621 tctgattttt ttatctcagg tttaaatgta aattgatagc caatgacagg ctttccccgt
    10681 ccttttccgt atctcttttg gattgtcaaa cctttgaaga ttgcggttag ttcttggcga
    10741 ataggcttta taattttttt atttatatca ctttctctat aacttttagg gatatcaagt
    10801 aattcatgaa agtcttcttt tgaaaaattg gcaattcctt tggttctgta ttgttttaat
    10861 aatctaaaca tggttttgga ataactgctt tctaattcat tgaattgttg gagagaatat
    10921 cgaacccatt cttcaagatt attaagtaag ggtaaggctt tctcatggac ttgtatttca
    10981 gcaaaaggaa tatcagcttt tccatcgatt ttaaattgat taaataaaac aaatcgttca
    11041 cgttgcaaac cgtcagcact tcttctacca aaacgtaaat ccattaattt gtcatacgtt
    11101 gtttctaaat catctatgaa tcgaacattt gcagttgctt tataattgct taaatctttt
    11161 aattgttcga atgtaaattg tactttttta tcgcctacat cacgcattct agatattata
    11221 gagaagaata aattcatttc tactgacgta aatttgcgta aaggaatcgt atttaattca
    11281 tggtggtatt tcacaatttc atttgccata aaatcctcct tcaagattaa ctacttaaca
    11341 aaattataaa gagctttcta atgggggtca accccctaat taaagacccc taaaaacaga
    11401 caaaaagacc cctaaaaaca gacaaaaaga cccctaaaaa cagacaaaaa gacccctaaa
    11461 aacagacaaa aagaccccta aaagattctg cacaccttgg gagagtaagg atacagccga
    11521 cggtaaagag gtttaaaaag aggttataaa agagattata aaagaggggg gcaaaatttc
    11581 ccgcctaaaa ttttcctact ttttttctcc aaacaggttc gcgaaatttc ctaaactggg
    11641 aatctgctcc ctacggtcgc tttaaaggtg tgatcgctac gctctcacat taaactgcgc
    11701 agtcgcctaa aggctccttt gcttaaaaac agttcgtaag gagtcctttt agaagctctg
    11761 agcgctcatc tacggcgttt taagacaagt ctagataaaa tcccttatct tgtttttaaa
    11821 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aacgttctac agaacctcta
    11881 aaattcgttt taaggcgttt taaagtcgct ttggtataat tacataccag aacgtttaaa
    11941 acgccttaaa acgcaaatta gagccctatg tatatatcga ctaatgttac ctagactttt
    12001 acggaagccg gcacactcat ttgcaaagct tcagagttgc aaatttggat tcgctcactc
    12061 gaaaactgat ttattttgac tctgatcaat aggtgtattt tttagaagga ttgctagcca
    12121 aattttgctt taatttgctc ataagtccca cccttttgat acgtccatag gacgtatcgt
    12181 ttgtaatgtg aaagtgacta taaggtccct ttctacaaaa cacactaaag ggcttggcaa
    12241 gctctatatt tgctagccaa atacctccgt atttggtgca aataaccgct tgtcaaaggg
    12301 gcgcaagctc ccccttgaat ccccctcaaa acccacgctc cacaatacaa aaagtggcta
    12361 gaaaatgcta gccacttttt gtaaaattta gtatgattta tttttagcta tttagagttt
    12421 ttatttccgt aaatacttct aaaaattgct aagataaatg ctataggaat tagataaaga
    12481 atactttttt gtaagcttat attgaaaatt agcctaaata tgaagaacat agtgattaaa
    12541 actattattg aaataattac agctaaaatc tttttattca tgagatagtt tcactttctg
    12601 attattttat tacataacga gcgtaatcat tgtattgata tgatgttgta ttgctaaagt
    12661 gagcattata tgttaattgt acaccttttc cttgatttgc agctcttaca aagacattca
    12721 ttgcatcaac agcactacta aaagcatttg taataaccca tgttccaact ccaccaatag
    12781 gaataaaaga tgcaatttga gcaggagtct ttagcttatt ataatttttt ttcaaatctc
    12841 tacacatttt attagtaaca taatagttca ttttatagca agtaaatcca cgatgattat
    12901 gattatatcg tgaacttgca gcttccactt ttatacctct agcactaaaa ggaaacagat
    12961 ttaacggagt acttgctcca acaatcatac cgaacaaaaa tagacaagta ataattttaa
    13021 cagaattagg cagaagtcgc ccatcctcta taggtgggtg atgaatgccg ttcggtatga
    13081 gggttaccgt tggtaactcg aagaccgaca ac
//
