LOCUS       NZ_CP017933            11638 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Klebsiella oxytoca strain CAV1015 plasmid pCAV1015-12, complete
            sequence.
ACCESSION   NZ_CP017933
VERSION     NZ_CP017933.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03733631
            Assembly: GCF_001870185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11638)
  AUTHORS   Mathers,A.J., Stoesser,N., Sheppard,A.E., Pankhurst,L., Giess,A.,
            Yeh,A.J., Didelot,X., Turner,S.D., Sebra,R., Kasarskis,A., Peto,T.,
            Crook,D. and Sifri,C.D.
  TITLE     Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae at
            a single institution: insights into endemicity from whole-genome
            sequencing
  JOURNAL   Antimicrob Agents Chemother 59 (3), 1656-1663 (2015)
   PUBMED   25561339
REFERENCE   2  (bases 1 to 11638)
  AUTHORS   Mathers,A.J., Stoesser,N., Sheppard,A.E., Pankhurst,L., Giess,A.,
            Yeh,A.J., Didelot,X., Turner,S.D., Sebra,R., Kasarskis,A., Peto,T.,
            Crook,D. and Sifri,C.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2016) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. unspecified
            Genome Coverage                   :: 120x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/04/2024 14:21:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,356
            CDSs (total)                      :: 6,238
            Genes (coding)                    :: 6,125
            CDSs (with protein)               :: 6,125
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 50 of 113
            Pseudo Genes (incomplete)         :: 74 of 113
            Pseudo Genes (internal stop)      :: 22 of 113
            Pseudo Genes (multiple problems)  :: 24 of 113
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP017933.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11638
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="CAV1015"
                     /isolation_source="Blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:571"
                     /plasmid="pCAV1015-12"
                     /geo_loc_name="USA:Virginia"
                     /lat_lon="38.14 N 78.45 W"
                     /collection_date="2007-08"
                     /collected_by="Clinical Microbiology University of Virginia
                     Health System"
     gene            complement(27..308)
                     /locus_tag="AGF18_RS31085"
                     /old_locus_tag="AGF18_31085"
     CDS             complement(27..308)
                     /locus_tag="AGF18_RS31085"
                     /old_locus_tag="AGF18_31085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000594459.1"
                     /translation="MIKNIKHKGLRSFAENGNTAGIQPHLAKKLRMILTTLSTATCLQD
                     IVDVRALGCHPLKGERKGEFSVEVNGNWRVVFKVEEPNVLDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            646..939
                     /locus_tag="AGF18_RS31090"
                     /old_locus_tag="AGF18_31090"
     CDS             646..939
                     /locus_tag="AGF18_RS31090"
                     /old_locus_tag="AGF18_31090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609270.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_000609279.1"
                     /translation="MINLYDPVHPGEFIREMFMEPFEIPAAELADKLEVSQSALSRLLN
                     GKTDLSYEMALRLSKVIGRSAESWVNMQAQYSLSKSRKTLSETLEHLHPMFP"
                     /name="HigA family addiction module antitoxin"
     gene            complement(2471..2686)
                     /locus_tag="AGF18_RS31100"
                     /old_locus_tag="AGF18_31100"
     CDS             complement(2471..2686)
                     /locus_tag="AGF18_RS31100"
                     /old_locus_tag="AGF18_31100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2803..3360)
                     /locus_tag="AGF18_RS31105"
                     /old_locus_tag="AGF18_31105"
     CDS             complement(2803..3360)
                     /locus_tag="AGF18_RS31105"
                     /old_locus_tag="AGF18_31105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897068.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_003100847.1"
                     /translation="MLIGYARVSTQDQNLELQREALSKAGCKKVFEDKVSGTRADRPGL
                     AKTLEMLREGDTLVVWKLDRLGRSVKQLVDLVGDLHKHGVQFRSLTDSIDTGTPSGRFF
                     FHVMASLAEMERELTVERTRAGLEVAKQLGRKGGRKPKMTDSKIESAKKLLASGVPPKD
                     VAKNLGVSIPTLYRWVPASTHA"
                     /name="recombinase family protein"
     gene            complement(3354..3725)
                     /locus_tag="AGF18_RS31110"
                     /old_locus_tag="AGF18_31110"
     CDS             complement(3354..3725)
                     /locus_tag="AGF18_RS31110"
                     /old_locus_tag="AGF18_31110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_003100853.1"
                     /translation="MSTSTIEALASAWARIAEEAEFPADYEGTATPQAHRASEAIQEQI
                     RERIVATNDMRLFSLLHLLGQASLRMEQALWPEDYERMTREVEEALRQATDANARSYTH
                     EEVMQAMQERIDRARDKPC"
                     /name="hypothetical protein"
     gene            complement(3722..4222)
                     /locus_tag="AGF18_RS31115"
                     /old_locus_tag="AGF18_31115"
     CDS             complement(3722..4222)
                     /locus_tag="AGF18_RS31115"
                     /old_locus_tag="AGF18_31115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_003100856.1"
                     /translation="MKLSFHQHIALFWMIGAPGVFAPVIENAKRPDAGAVMAWGVAIVA
                     VMILFTPLLLRCPPFRRWYGRTDALSERQRQALAERGLRRYYQTAFDDGYVPRVMPYVW
                     RIIWTVGGLMAVTAVLPTNTGRPAFDALVVFSTWYPIGVMLLVFASRPLGRLIRQRAQE
                     RRK"
                     /name="hypothetical protein"
     gene            complement(4219..4545)
                     /locus_tag="AGF18_RS31120"
                     /old_locus_tag="AGF18_31120"
     CDS             complement(4219..4545)
                     /locus_tag="AGF18_RS31120"
                     /old_locus_tag="AGF18_31120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_003100858.1"
                     /translation="MSRSRRKTPIVGHTTCGSEREDKKLWHQRWRTRERTALTSASPEA
                     LSAHLPLLENQASSVWSMGKDGRSYWPVKRQAATADRIANHKGRNPQERASLKKRLLRK
                     WMSK"
                     /name="hypothetical protein"
     gene            complement(4800..5156)
                     /locus_tag="AGF18_RS31125"
                     /old_locus_tag="AGF18_31125"
     CDS             complement(4800..5156)
                     /locus_tag="AGF18_RS31125"
                     /old_locus_tag="AGF18_31125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_000215515.1"
                     /translation="MTVESRIFSVAEYVQPSEGEPIRSVVLETRDSIIVVWHVHPGQEI
                     AAHIHPHGQDTWTVLSGMADYFQGNGIVRALREGEIAVARPGQVHGARNTGTEPFVLVS
                     VVASANAGFVLAER"
                     /name="cupin domain-containing protein"
     gene            complement(5146..5547)
                     /locus_tag="AGF18_RS31130"
                     /old_locus_tag="AGF18_31130"
     CDS             complement(5146..5547)
                     /locus_tag="AGF18_RS31130"
                     /old_locus_tag="AGF18_31130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="WP_001293886.1"
                     /translation="MSENRLPDYLDHIQQAATDARSFVEGMAKDDFLADKRTQQAVIMS
                     LIVIGEAATKVMDGYVEFTQAHADVPWRSMRNMRNRMAHGYFDINLDVVWETVQEWLPA
                     LLQQLPAVRQDADDEDRNDNCERGISDDC"
                     /name="DUF86 domain-containing protein"
     gene            complement(5544..5834)
                     /locus_tag="AGF18_RS31135"
                     /old_locus_tag="AGF18_31135"
     CDS             complement(5544..5834)
                     /locus_tag="AGF18_RS31135"
                     /old_locus_tag="AGF18_31135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="WP_001247892.1"
                     /translation="MRPSVVLDMKRSAVREAVGRFRAANPRVFGSVLHGTDRDGSDLDL
                     LVDALPGATLLDLGDLEEELKSLLGVDVDLLTPGDLPPKFRAKVLAEAQPI"
                     /name="nucleotidyltransferase"
     gene            5993..8959
                     /locus_tag="AGF18_RS31140"
                     /old_locus_tag="AGF18_31140"
     CDS             5993..8959
                     /locus_tag="AGF18_RS31140"
                     /old_locus_tag="AGF18_31140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834018.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element ISPa38 family transposase"
                     /protein_id="WP_047719753.1"
                     /translation="MPRRSILSAAERESLLALPDTKDELIRHYTFSESDLSIIRQRRGP
                     ANRLGFAVQLCYLRFPGVILGADEPPFPPLLRLVANQLKVGIESWDEYGQREQTRREHL
                     VELQTVFGFQPFTIGHYRQAVQLLTELAMQTDKGIVLARALIEHLRRQSVIVPALNAVE
                     RASAEAITRANRRLYDALAEPLTDVHRRRLDDLLKRRDNGKTTWLAWLRQSPVKPNSRH
                     MLEHIERLKAWQALDLPSGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYATLVAL
                     AIEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLFGRIGQALIEA
                     KQAGRDPFAAIEAVMSWDAFAESVTEAQRLAQPEDFDFLHRIGESYATLRRYAPEFLDV
                     LKLRAAPAAKDVLDAIEVLRSINSDNARKVPTDAPTEFIKPRWQKLVMTDTGIDRRYYE
                     LCALSELKNALRSGDIWVQGSRQFKDFEDYLVPPAKFASLKQASELPLAVATDCDQYLH
                     DRLTLLETQLATVNRMALANELPDAIITESGLKITPLDAAVPDTAQALIDQTAMILPHV
                     KITELLLEVDEWTGFTRHFAHLKSGDLAKDKNLLLTTILADAINLGLTKMAESCPGTTY
                     AKLAWLQAWHIRDETYGAALAELVNAQFRHPFAGHWGDGTTSSSDGQNFRTGSKAESTG
                     HINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGVRDSTYVLDGLLYHESDLRIEEHYTD
                     TAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDATYEALKPMIGGTLNIKHVRAH
                     WDEILRMATSIKQGTATASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVEL
                     RRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYL
                     ERAANALRGHGQAVDDGLLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
                     /name="Tn3-like element ISPa38 family transposase"
     gene            complement(8966..9412)
                     /locus_tag="AGF18_RS33925"
     CDS             complement(8966..9412)
                     /locus_tag="AGF18_RS33925"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_227518097.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLIGVVSENGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<9447..10151)
                     /locus_tag="AGF18_RS34100"
                     /pseudo=""
     CDS             complement(<9447..10151)
                     /locus_tag="AGF18_RS34100"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(10090..10413)
                     /locus_tag="AGF18_RS31150"
                     /old_locus_tag="AGF18_31150"
     CDS             complement(10090..10413)
                     /locus_tag="AGF18_RS31150"
                     /old_locus_tag="AGF18_31150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_171853543.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMTIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(10527..10700)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          10624..11211
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.54"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(2380..2768)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.692"
                     /coverage="100.0"
                     /evalue="2.39e-71"
     Typing          complement(8995..10100)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.935"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 tggattagta acttacttat tgattactaa tggtaatctt caagatcaac gtctaagacg
       61 tttggttctt ccaccttgaa aaccaccctc cagtttccgt taacctctac ggaaaactca
      121 cctttacgtt caccttttag ggggtgacac cccagcgccc ttacatccac aatatcttga
      181 agacatgttg cggtgcttag cgtagtcaat atcatgcgaa gttttttcgc taagtgaggc
      241 tgaattccgg cggtgttacc gttttcagca aaactccgaa gccctttatg ctttatgttt
      301 tttatcatca atcatctcca taaaattttc gtcgatatct atctctataa acagaaaaca
      361 atttgacagc gtccatttga accccttgca ttattaaaat aattgacgta actttgtctc
      421 tgaggtcgat tacctcgttt gacttccaag agcgctaacc gtacaatatg gcgccttgaa
      481 aaagtcggta aagacaccat atcagcggta gctttctgct ccaccgagca tcctaaagct
      541 tactgctttt tttgtcaata acagacactt gcgtattgtg caatgcgcaa cgcgaaagaa
      601 acaaaatgtt ttgtgtaatc aaatacaaaa ccaatagagg aaggcatgat aaatctttac
      661 gatcctgtac accccggtga attcatcagg gaaatgttca tggaaccgtt cgaaatacct
      721 gccgcagagt tagctgataa gcttgaagta tcacaatctg cattaagtag attgctcaat
      781 ggaaaaactg atcttagtta tgagatggct cttcgactat ctaaagttat aggccgctct
      841 gcagagtcgt gggttaatat gcaggctcag tacagcctct cgaaaagtcg taaaacttta
      901 tccgagacgc tagaacacct tcatccgatg tttccataag taaaagcccg ctcgtgcggg
      961 ctttttgccg taaaaccccc gcatacctac ctttagtttg atttttagtt cagttcttag
     1021 atcttgaaat gattgttgtt tgaacaccaa taagatgccc atacaaggct ctaaaaagga
     1081 gaacaaaatg gcaatttatg taggaccaca tggcaaaata ggtcgtgatg gtggctcatt
     1141 ccaagaatgt gacatcaaca caaacactca tactgttacc cgcaaatatg gacgatactg
     1201 caccattcgc gataatgagt tcgcaccacc aacgagcgca cctggcaggt actggctaca
     1261 gatctaccga accccagaca acactgagta acagaagcgc cagtaacgac aaagcccgca
     1321 taagcgggct ttgtctctta caaaattcct cgtcgtcgca attcagcttc tatcgcctca
     1381 catgcggcat ccacaatctc cgtgttgtag tacctggaca tgccactgct aatgatatcg
     1441 tccattaggg cacggttatg agctaaggta cgtcgtaagt attcaggggt atgttgctcc
     1501 atcggattct ccttttcatg actgagcatt attgcttacc tgtatttatc cccgtcagtg
     1561 ctggtcacgt tgaaaagccc taaaaaacca cttttttagc tgccttaatc attgaaaaat
     1621 ggcacttaaa tccacatcta tcgcgctggg gaaatcgccc gcacacagcc actgataaaa
     1681 taaaagtgag ataaaatgga tccaatggat aagatttgcg atgtttgtgc cctccctgcc
     1741 agcgcctgta cctgtggctt ctgcgatgac tgcaacgaga agctggcaga ctgcgtctgt
     1801 gagcgctgca gcgattgtgg aaatttagat gagcactgta gctgctcacg ttgcgacgag
     1861 tgcggtgaaa aactggatga gtgcatatgt gaggagtgcg aagaatgtct ccgcagtcca
     1921 catgattgta tctgcgaaaa ctgacctaag gccgaattct ctttcaagag cgaccgggac
     1981 ggtcgctctt atttaccata acatccgttg ggcgcaccgc tgaaacgggg ttggctgcgt
     2041 ttccggtgcc agacgcgaga aagtggcgac agacataacg aaaaaacacc agaaacgcag
     2101 ccgctgaata ttacactggt gcatagtcat gcagccagat gcatggatgt taaagtcaca
     2161 ctaaaatccg cgccaatgcc ttaaaaaccg acaaatgctt ctccagccag tcccacagct
     2221 ccgccgccag atacaggcag ttaagcaatg attcaataag atttaacagc ttcatttccc
     2281 gctcctgaat aagtcgtgac ctttttgatc acgaacctat ttagcgggag aaataagagc
     2341 ccctcttcgt gcgggttgtc agctgcctgg atggtttaaa gtgtcggccc gcggtaggtt
     2401 ttctgcggct ggcgctccag ctccagtgtt ttctgatact cattctccct ctgccggagc
     2461 tcgatggcat tcagttcccg gacaagcggc gcagctgctc gcttaaactc agcacctgcg
     2521 tgctcaagtc cgacacgcgc tcgttcagca ccgcgttctc ccgttgcata agcccacaca
     2581 tgctccgcca ttccgcgaag gcgctctccc attcgtccag cctttgcgag tagtcctgct
     2641 gtagctgctc taatgcgctc agcaactgct tttccagctc tgtcattttc agctactcct
     2701 gtttcaggcg gatccatgtc gtcccgtcgt aagggatggc ttcggtttgt atccccggcg
     2761 gcaggataag gggtcgtctc agaaaacgga aaataaagca cgctaagcgt gcgtggaggc
     2821 tggcacccag cggtacagcg tcggaatgga cacgccgagg ttcttggcca cgtccttggg
     2881 cggcaccccg ctggccagca gcttcttggc cgactcgatc ttgctgtcgg tcatcttcgg
     2941 cttgcggccg cctttgcggc cgagctgctt ggcgacttcc agcccggcgc gggtgcgctc
     3001 gacggtcagc tcgcgctcca tttcggcaag gctcgccatg acgtggaaga agaaccgccc
     3061 ggatggtgtg ccggtgtcga tggagtcggt gaggctcctg aactggacac cgtgcttgtg
     3121 cagatcgccg accagatcga ccagttgctt gaccgaccgg cccagccggt cgagcttcca
     3181 gacgaccaaa gtatcgcctt cgcgcagcat ttcgagcgtc ttggccaagc caggccggtc
     3241 tgcccgcgtg ccactcacct tgtcctcgaa gaccttttta catccggcct tgctcaaggc
     3301 ttcgcgttgc agctccaggt tctgatcctg cgtcgagacg cgcgcatagc caatcaacat
     3361 ggcttgtctc gcgcccggtc gatgcgttcc tgcatcgcct gcatcacttc ttcgtgggtg
     3421 tacgatctgg cgttggcgtc ggtggcttgc cgcagggctt cctcaacctc gcgcgtcatc
     3481 cgctcgtaat cctccggcca cagcgcttgc tccatgcgca gcgacgcctg acccagcagg
     3541 tgcagcaggc tgaacagccg catgtcgttg gtggcgacga tgcgctcccg aatctgctcc
     3601 tgaatagctt cgctagcccg atgcgcttgt ggtgtggcag tcccctcgta gtcagcgggg
     3661 aattccgctt cctcggcaat ccttgcccag gcgctggcca gcgcctcgat ggttgacgtg
     3721 ctcatttccg ccgctcctgt gctctttggc gaatcaaccg gccaaggggc ctcgacgcga
     3781 aaaccagcag catcacgcct atcggatacc aggtcgagaa gaccaccaag gcatcgaagg
     3841 ctggccgtcc ggtgttggta ggcagtacgg cggtcacagc catcaacccg ccgaccgtcc
     3901 agatgatgcg ccacacgtag ggcatcacgc ggggcacgta gccgtcatcg aaagcggtct
     3961 ggtagtagcg gcgcaggccg cgctcggcaa gcgcctggcg ctgccgctcc gacagcgcat
     4021 ccgtccggcc ataccagcgc cggaatggtg gacagcgcag cagcaaaggg gtgaagagga
     4081 tcatcaccgc cacgatcgcg acaccccacg ccatgacggc gccggcatcg ggccgcttgg
     4141 cgttctcgat cacgggcgcg aagacgcccg gagcaccgat catccagaac agcgcaatgt
     4201 gctgatggaa ggagagcttc atttgctcat ccacttgcgc agcaggcgct ttttcaggga
     4261 ggcgcgttct tgcgggttgc gtcccttgtg attggcgatg cgatccgccg tggcggcctg
     4321 gcgcttgacg ggccagtagg agcggccatc cttgcccatc gaccagacgc tgctggcctg
     4381 gttttccagc aggggcagat gggcgctcag ggcttcgggc gacgcgctgg tcagcgccgt
     4441 gcgctcacgg gtgcgccagc gctgatgcca gagcttcttg tcctcgcgct cgctgccgca
     4501 ggtcgtgtgc ccgacgatgg gtgttttgcg gcggctgcgg ctcataacgt agtggtctcc
     4561 aagaacattc aggactgatc ccaggcgtcg atggtcaaga cctgatcggc aggacaggcg
     4621 gctacgcggt cgggaactgg atggtgttca gtctcatgcc gaccccattc gattgatcgc
     4681 gtcgcttgcg gcacgctgcg acgcaaggag gtttgcgacc tggtttagca gccgtgtccg
     4741 ctgcatgtct gttacctatc tccccgaccg ctcattggac caactccaga gattgggctc
     4801 tatcgctcag ccaatacgaa accggcattg gctgatgcca caaccgagac taacacaaat
     4861 ggctcggtac ctgtatttcg cgccccgtgc acttggcccg gtcttgccac ggctatctca
     4921 ccttccctga gggcacgaac aatcccattg ccctgaaagt aatcagccat tcccgacaaa
     4981 acagtccacg tgtcttggcc gtgaggatga atgtgagccg caatttcctg cccgggatgg
     5041 acatgccaaa ccacgataat tgagtctcgg gtttcaagca caacggaacg aataggctcg
     5101 ccttcggacg gctgaacata ctcggctaca gaaaatattc tcgattcaac agtcatcgga
     5161 gatccctctt tcacagttgt cgttacggtc ttcatcgtcg gcatcctgac gcacggcggg
     5221 caattgctgg agcaacgccg gcagccattc ctgtaccgtc tcccacacca catcgaggtt
     5281 gatgtcgaaa tagccgtgag ccatgcgatt acgcatattg cgcatgctgc gccacggcac
     5341 gtcggcatgc gcctgggtga actcgacgta gccatccatc acctttgtgg ccgcctcgcc
     5401 gatgacgatc aggctcatga tgacggcctg ctgggtgcgc ttgtcggcca agaagtcgtc
     5461 cttggccatc ccttccacga agctgcgcgc atcggttgcg gcctgctgaa tgtggtcgag
     5521 gtaatcgggc aggcggttct cgctcatatc ggttgcgcct ccgcgagcac cttggcccgg
     5581 aacttcggcg gcaggtcgcc gggagtcagc agatcgacgt caacgccgag cagcgatttc
     5641 agttcttctt ccaaatcgcc caagtccaac aacgtggcac cgggcagcgc atcgaccaac
     5701 aggtcgaggt cgctgccatc ccggtcggtg ccatgcagca ccgagccgaa gacgcgcggg
     5761 ttcgcggcgc gaaagcggcc taccgcttca cgcactgcgc ttcgcttcat gtcaagcaca
     5821 acagacggtc gcatgcgcat cctttcttat cgaaactcgt tgagatgata tgcaatcaag
     5881 aatagaattt caagaactat tttcgagaat cgcaatgcct tgattcccgt gccgcgccgg
     5941 ttgccttggc gggcttgtca caaacctacg ttttcaagaa gaaggaaacc gcatgccccg
     6001 ccgttcgatc ctctccgccg ccgagcgcga aagcctgctg gcgttgccgg acaccaagga
     6061 tgagttgatc cgtcactaca cgttcagcga aagcgacctc tccatcatcc ggcagcggcg
     6121 cggcccggcc aatcggctgg gcttcgcggt gcagctctgc tacctgcgct ttcccggcgt
     6181 catccttggc gctgatgagc caccgttccc gccattgctg agactggtcg ccaaccagct
     6241 caaggtcggc atcgaaagct gggacgagta cgggcagcgt gagcagaccc gacgcgagca
     6301 cctggtcgag ctgcaaacgg tgttcggctt ccagccgttc acgattggcc actaccggca
     6361 ggctgtccag ttgctgaccg agctggccat gcaaaccgac aagggcatcg tgctggccag
     6421 agccttgatc gagcacctgc ggcggcagtc ggtcattgtg cccgccctca acgccgtcga
     6481 gcgggcgagc gccgaagcga ttacccgcgc caaccggcgt ctctacgacg ccttggctga
     6541 gccgctgacg gacgtgcatc gccgtcgcct cgacgatctg ctcaagcgcc gcgacaacgg
     6601 caagacgacg tggctggcct ggctgcggca atccccggtc aaaccgaact cgcggcacat
     6661 gctggaacac atcgaacgcc tcaaggcgtg gcaggcgctc gacctgccct ccggcatcga
     6721 gcggctggtt caccagaacc ggctgctcaa gatcgcccgc gagggcggcc agatgacgcc
     6781 cgccgacctg gcgaagttcg agccgcagcg gcgttacgcg accctggtgg cgctcgccat
     6841 cgagggcatg gccaccgtca ccgacgaaat catcgacctg catgaccgca tcctgggcaa
     6901 gctgttcaat gccgccaaga acaagcatca gcagcagttc caggcatccg gcaaggcgat
     6961 caatgccaag gtgcggctgt tcgggcgcat cggccaggcg ctgatcgagg ccaagcaagc
     7021 gggccgcgat ccgttcgccg ccatcgaggc cgtcatgtcc tgggatgctt tcgccgagag
     7081 cgtcaccgaa gcgcagcggc tcgcgcaacc cgaggacttc gatttcctgc accgcatcgg
     7141 cgagagctac gccacgctgc gccgctacgc gccggaattt ctcgacgtgc tcaagttgcg
     7201 ggccgcgccc gccgccaagg acgtactcga cgccatcgag gtgctgcgca gcattaacag
     7261 cgacaacgcc cgcaaggtgc ccaccgacgc gccgaccgag ttcatcaagc cgcgctggca
     7321 gaagctggtg atgaccgaca ccggcatcga ccggcgctac tacgaactgt gcgcgctgtc
     7381 ggagctgaag aacgcgctgc gctccggcga catctgggtg caaggctcgc gccagttcaa
     7441 ggacttcgag gactacctgg tgccgcccgc gaaattcgcc agcctcaagc aggccagcga
     7501 attgccgctg gccgtggcca ccgattgcga ccagtacctg catgaccggc tgacgctgct
     7561 ggaaacgcag ctcgccaccg tcaaccgcat ggcgctggcc aacgagctgc cggacgccat
     7621 catcacggag tcgggcctga agatcacgcc gctcgatgcg gcggtgcccg ataccgcaca
     7681 ggccctgatc gaccagacgg cgatgatcct accgcacgtc aagatcaccg aattgctgct
     7741 ggaggtagac gaatggacgg gcttcacccg gcacttcgcc cacctgaagt caggcgacct
     7801 ggccaaggac aaaaacctgt tgctgaccac gatcctcgcc gacgcgatca acctgggcct
     7861 gaccaagatg gcggaatcgt gccccggcac gacctacgcc aagctggcct ggctgcaagc
     7921 ctggcacatc cgcgacgaaa cctacggggc ggcactggcc gagctggtca acgcgcagtt
     7981 ccgacatccc ttcgccgggc attggggcga cggcaccacg tcatcgtcgg acggccagaa
     8041 cttccgcacc ggcagcaagg ccgagagcac cggccacatc aatccgaaat acggcagcag
     8101 cccagggcgg acgttctaca cccatatctc cgaccagtac gcgccgttcc acaccaaggt
     8161 cgtgaacgtc ggcgtgcgcg actcgaccta cgtgctcgac ggcctgctgt atcacgaatc
     8221 cgacctgcgg atcgaggagc actacaccga cacggcaggg ttcacggacc acgtcttcgc
     8281 gttgatgcac ctgctgggct tccgcttcgc cccgcgcatt cgtgacctgg gcgacaccaa
     8341 gctctacatc ccgaagggcg atgccaccta cgaggcgttg aaaccgatga tcggcggcac
     8401 gctcaacatc aagcacgtcc gcgcccattg ggatgaaatc ctgcggatgg ccacctcgat
     8461 caagcagggc acggcgacgg cctcgctgat gctcaggaag cttggcagct acccgcgcca
     8521 gaacggcctg gccgtcgccc tgcgtgagct gggacgcatc gagcgcacac tgttcatcct
     8581 ggactggctg caaagcgtcg agctgcgccg ccgcgtgcat gccgggctga acaaaggcga
     8641 ggcgcgcaac gcgctggccc gcgccgtgtt cttcaaccgc ctgggggaaa tccgcgaccg
     8701 cagcttcgag cagcagcgct accgggccag cggcctcaac ctggtgacgg cggccatcgt
     8761 gctatggaac acggtctatc tggagcgggc cgcgaacgcc ttgcgtggcc acggtcaagc
     8821 cgtcgatgac ggcctgttgc agtacctgtc gccgctcggc tgggagcaca tcaacctgac
     8881 cggcgattac ctctggcgca gcagcgccaa gatcggcgcg ggcaagttca ggccgctacg
     8941 gccgctgcaa ccggcttagc gtgctttatt ttccgttttc tgagacgacc ccgataagaa
     9001 acctccggcc gtcgctgctc tcctgatagc gcactcccca cgtccgggcg gtcattttcg
     9061 ccagggtatc ttcctgctga cgaagatgcg tgtaattgtc ggttatctgc tgcccctgcc
     9121 accacagaat gctcccgctc gtgccgatca gcagcgctga caccatcagg atggtgagcc
     9181 atgtccgccc ggcagtccgc agtacgctcc gccggttttt ctccatcgcc tcgctcatac
     9241 tctgctcgtg ggcgaggatg gcgtcgctga ttctcctcgc gcttgcgctc agttccgctc
     9301 tgacagactg ctcgtgctcg ctgaatgcgg ctttcagcat ctcgccggta ctctgctgct
     9361 gcgctttcga ttgctgctct aagtccttcg ccagcgttaa aagactgctc atagatggct
     9421 cccttcagtc ggatgtttcg ccccccgtcc gggtcggcaa tgctgatact gcttttcgtg
     9481 gtgcgcacca cctcaaaacc tgcaccttca agagtgtcag tgacatcctg acggcttttc
     9541 agctccccgg acgaagcaag ggtcagcagt ccccgggtaa tggcttctgc ggcctcctgc
     9601 ttcgttttcg gcagcccgga cggcgtgacc agagcccggc ggttttccgg cgcgttcggg
     9661 tcgtgcagcc ccagtctgcc gttcacgata gtctgccagg catcgatgcg cggccggtcg
     9721 gcgcggtcgt agtaaggctg gagccgcctg ccggtcagca gctcggtatt ggggatcaga
     9781 aagttcagct ccagacgccc cttgtccgcg tgctccaccc acagaatgct gtactgatct
     9841 ttatcgagtc cgggcatcag aacccgctcg aagctggcca tcagtttttc ccgctggccg
     9901 ggaggcagat cggcctccgc aaacgacaga acgccggagg tgtatttctt aacgtaaggc
     9961 gacgcgtcaa tcagctcccg gacttcttcc ggcttcccct gcaggacggt ggcgccttca
    10021 cgctgccggt ctttacccag cagatagtcc accggcccgg cgccaccgcc gcggccgcgc
    10081 ggatgaaatt taacgatcat cgtccgttcc tttctcaaga acggcgtgcc gcagccgctc
    10141 aaggccggca tcgatggtca tcagcgccgc cacaatctgt accctgtcat ggccggtgcc
    10201 gccgccggcg ttaacacggc gggctatctg gttcaggtta ttgcccatgc cggcaagctg
    10261 gcgcagcagc gccggcgaga gtgaggggag ctttccggaa cgtgccggct tttcgtccag
    10321 gcagatctgc cgcatccacg ccgccagctg cctgccgtca cagcgctcga gcagccgccg
    10381 gtgctcgtcc tccgtcaccc acatggtgag catcttgctc cgtttctctg acaaaccggc
    10441 ctcctgataa cgccgggacg ggcgggaaag cctttcccgg tcggcgcggg gttggacaag
    10501 ccgcaggcgc gtcaggcttt tggcgggttt cggggcgcag ccctgaacca gtcacgtagc
    10561 gctagcggag tgtatactgg cttactatgt tggcacggat gagggtgtca gtgaagtgct
    10621 tcatgtggca ggagaaaaaa ggcggcaccg gtgcgtcagc agaatatgtg atacaggata
    10681 tattccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggaa
    10741 atggcttacg aacggggcgg aaatttcctg gaggatgcca ggaagaagct taacagggaa
    10801 gcgagaaggc cacgacaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg
    10861 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
    10921 ttccccctgg tggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
    10981 ctccgctgtt atggccgcgt ttatctcatt ccacgcctga cactcagttc cgggaaggca
    11041 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg cagcgtctta
    11101 tccggtaact atcgacttga gtccaacccg gaaagacaca acaaaacgcc actggctgcg
    11161 gccattggta agagggtgta caagagatta ctgatgcaga gttcttgaag tcagagccat
    11221 actgaggcta aactggaagg acagaatttg gtttcagcgg tcttgtacac cctgttacca
    11281 cggttcagaa gttccccaac ttactgaacc ctcgaaaaaa ccgcctcccc aggcggtttt
    11341 ttcgtttcga acataagata cgctttgtcg atttaatcag tcaaggaaga accctgaaag
    11401 cctcaatcgc ttacagaatt attcttgtta gagacatgtt tctgcaccgt atcaacacca
    11461 gtacacaaaa cagctgcaaa caggaaccat ccccacccat ctaggccacg aacagcaaga
    11521 accccagcaa tgactacaca aagcaaaggg aacaacaaaa tcataaaggc tctcataaat
    11581 cctccagagg tacttatttt tgaataaagt ccctaatcca tccatattgc cactagga
//
