LOCUS       NZ_CP017845             5538 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain FMU073332 plasmid pEcoFMU073332a, complete
            sequence.
ACCESSION   NZ_CP017845
VERSION     NZ_CP017845.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05945146
            Assembly: GCF_001888075.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Saldana-Ahuactzi,Z., Cruz-Cordova,A., Porta,H., Cevallos,M.A.,
            Eslava-Campos,C., Navarro,A. and Xicohtencatl-Cortes,J.
  TITLE     Genome Sequence of the Enterotoxigenic Escherichia coli strain
            FMU073332
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   Cevallos,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2016) Programa de Genomica Evolutiva, Centro de
            Ciencias Genomicas, Av. Universidad s/n, Col. Chamilpa, Cuernavaca,
            Morelos 62210, Mexico
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: HGAP_3 in SMRT analysis v. 2.3, Spades v. 3.5.0
            Genome Coverage                   :: 290X
            Sequencing Technology             :: Illumina HiSeq and PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001888075.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 02:27:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,014
            CDSs (total)                      :: 4,891
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 356
            CDSs (without protein)            :: 356
            Pseudo Genes (ambiguous residues) :: 0 of 356
            Pseudo Genes (frameshifted)       :: 95 of 356
            Pseudo Genes (incomplete)         :: 258 of 356
            Pseudo Genes (internal stop)      :: 63 of 356
            Pseudo Genes (multiple problems)  :: 49 of 356
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP017845.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FMU073332"
                     /serotype="O6:H16"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEcoFMU073332a"
                     /geo_loc_name="Mexico: Tlaltizpan, Morelos"
                     /lat_lon="18.688857 N 99.119482 W"
                     /collection_date="1987"
                     /collected_by="Carlos Eslava Campos, Armando Navarro Ocana,
                     Alejandro Cravioto"
                     /genotype="st+, lt+, lngA+, cstH+, ecpA+, etpA+, etpB+,
                     tibA-, leoA-, tia-, eatA-, cfAI, cfAIII-, cs1-."
     gene            join(5355..5538,1..155)
                     /locus_tag="BLJ80_RS25760"
                     /old_locus_tag="BLJ80_23505"
     CDS             join(5355..5538,1..155)
                     /locus_tag="BLJ80_RS25760"
                     /old_locus_tag="BLJ80_23505"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000818065.1"
                     /translation="MKRERMLTIRVTDDEHARLLERCEGKQLAVWMRRVCLSEPVARSG
                     KLPTLAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAVLMAIERELRSLRQVVR
                     EHGARDDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            364..>795
                     /locus_tag="BLJ80_RS30345"
                     /pseudo=""
     CDS             364..>795
                     /locus_tag="BLJ80_RS30345"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            833..1351
                     /locus_tag="BLJ80_RS29515"
     CDS             833..1351
                     /locus_tag="BLJ80_RS29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015059036.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTTSTEESLEKHREGVKEAMMQHRKDVLKLAGNTGMRLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            1358..1591
                     /locus_tag="BLJ80_RS29520"
     CDS             1358..1591
                     /locus_tag="BLJ80_RS29520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137441.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2160..2984)
                     /locus_tag="BLJ80_RS25780"
                     /old_locus_tag="BLJ80_23520"
     CDS             complement(2160..2984)
                     /locus_tag="BLJ80_RS25780"
                     /old_locus_tag="BLJ80_23520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000213997.1"
                     /translation="MTTTIYREFEFAACDSKWSVDGHLRRDVIEKKAIHIDFKGKEAIS
                     LMCGDHPMIVLMQAMQLKVITPEQYASLMSLYMDVEDFTFGMVVLDKHSGAILVGMYTP
                     PMIAIKSKQIVYDLGSTGGEFAAHCLYYAEKRLRKKKRHRGYSTSSVGCKITSAVKYAY
                     RRDRYSGGKVYKIIWKNNGIMYNNIPLSNNSVKNHYRRILKETLKIIHSRYEEKINMRQ
                     NAQLNTAAASLSQTGQPKTQSQPHNQAPSASADKSHQMTMSRAINYARLFSD"
                     /name="hypothetical protein"
     gene            complement(3040..3576)
                     /locus_tag="BLJ80_RS25785"
                     /old_locus_tag="BLJ80_23525"
     CDS             complement(3040..3576)
                     /locus_tag="BLJ80_RS25785"
                     /old_locus_tag="BLJ80_23525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032328591.1"
                     /translation="MKDNKAAVVFTGKDLNIMRAEGGSGYWLAKAERLNDAKYLIAVRN
                     RRETWAVKDLEHGTGFLIAKITGCFKNPDYDDRNVITFEEYAEISVKKAWKMLTGGQRY
                     PVAYLTAEEAFSRIGITLEQLEWKKLQTSTLSQPIPSAEAPKKLSLNEAVERAKQDISN
                     ATDIDVSSITITINI"
                     /name="hypothetical protein"
     gene            complement(5114..5305)
                     /locus_tag="BLJ80_RS25810"
                     /old_locus_tag="BLJ80_23530"
     CDS             complement(5114..5305)
                     /locus_tag="BLJ80_RS25810"
                     /old_locus_tag="BLJ80_23530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          4941..5022
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="95.0"
                     /evalue="1.44e-35"
     Typing          complement(4371..4919)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.756"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          146..1695
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.603"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5338..5537
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.015"
                     /coverage="99.0"
                     /evalue="3.9e-35"
ORIGIN
        1 ggaataacct gaatcagacg gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc
       61 gggttcaggt ggtggccgta ctgatggcca tcgagagaga gttgcgcagc ttacggcagg
      121 tcgtcaggga acatggagcg cgggatgata gttaagtttc atgccagggg aaaaggtggt
      181 ggcagtggtc cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg
      241 cttcagggta atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa
      301 tacacgtcag gtgttctgtc gttcgcggag aaggtgctgc cgccgggagg acgtgaaaaa
      361 gtgatggcga gctttgagcg cgtactgatg cccggtctcg aaaagaatca gtacagcatc
      421 ctgtgggtgg agcaccagga caagggacgg cttgagctta attttgtcat tccgaacatg
      481 gagctacaga gcggaaaacg cctccagccg tactacgacc gggcagacag gccgagaatt
      541 gatgcctggc agacgctggt gaatcaccat tacgggctgc atgaccccaa cgccccggag
      601 aaccgccgga tactgactct ctctgataac ctgccggaaa cgaaacaggc gcttgctgag
      661 agcgttacac ggggaataga cgctctttat catgtcgggg agataaaagg ccgtcaggat
      721 gtgattcagg cgctcacaga ggcggggctt gaagttgtca gggtgacgcg aagcagtatc
      781 agcattgcag acccgaacgg cgggaagaat atcaggctga aaggagcatt ttatgagcaa
      841 tcttttacag acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga
      901 gacaatgctg aacgacgagt tcagcaagct cgaaaaatct gtaagcaagg ctgtgacctc
      961 aaacgagacg aaaatcaaag acgctatagc cctgttcaca acgtcgacag aggaatcgct
     1021 ggaaaaacac cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa
     1081 gctggcaggg aatacgggca tgaggttgct ggggatagtc tttctcctgt ttaccgcgag
     1141 tggcgggacg ctctggtatc ttggcgggcg gatacaggcg aacctggaag aaatcaggat
     1201 acaggaagaa acattgcaga aactgaacgc gaagacatgg ggcgtggagt ttgtgcagga
     1261 cgggagaagg aaatttctgg tccttccgta cgggaaatcg gcaacggtga ttccctatca
     1321 ggggaaggac tgggtacaac tgacggagtg acacagagtg acggagctgg aaagacattt
     1381 gctgaacgca ttagagcaac tgcaacagga ctatatgcag cggctgagcg aatgggagag
     1441 cgccttcgtg gaattgcaga aaatgttttc gcttacgcaa cgggacaacg cgatgctgaa
     1501 cgagcaggtc atgcagttga gtcagcaggt gcagcacttg agcgagcgga cagaacgctt
     1561 gagccagtta tacagcgaga acaggagata agggacgaac ggctgataca ggagcgcgaa
     1621 catgcgttat ccctggagcg tgagcgccag ccggaaatac aggaacgcac actggatggc
     1681 ccttcgctgg gatggtaatc agagacaggc taaaatgttg tcagcgcgtt tccggtgcat
     1741 tttttgggat aatggttggt tcgggggcac cgggaacgcg cttcagtcag accttgctga
     1801 aaacacagga acgcgttaaa acgcatacgg gcgcgattct ggtgtgtctt ttgttgctgt
     1861 gttttgcctc tattgccaga tcgcgctcat tcccctgaac ttctgttttt ccgctccggt
     1921 tccgtcttct gcctttccga aagtatgaaa aaaatccgtt tcagcagtct ctggctggca
     1981 attcctgcgt gattcacatg gctgcatagc tatgcatgtg gttaaaattt atcgggtgcg
     2041 atcgcggcag tttttcgggt ggtttgttgc catttttacc tgtctgctgc cgtgatcgca
     2101 ccgaacgcgt ttctgcggtg cacgcaataa gggattatgg taaatctagc tgtacagtat
     2161 cagtcagaaa aaaggcgagc atagttaatg gctcgagaca tagtcatctg atggctttta
     2221 tcagcactcg cactaggtgc ttggttatgt ggctgacttt gtgttttcgg ctgacccgtc
     2281 tgagagagcg aggctgccgc tgtatttaac tgggcattct gtctcatgtt tattttctct
     2341 tcgtacctgc tatgaatgat ctttaatgtt tcttttaaaa tccgccggta atggtttttt
     2401 accgagttgt tagataacgg tatgttgttg tacatgatgc cgttattttt ccaaatgatt
     2461 ttgtatactt taccaccgga gtagcgatct cgtctgtatg catatttgac agcgctggtg
     2521 atcttacatc ccactgacga tgtagagtat ccacgatgac gctttttttt tctaagcctt
     2581 ttttccgcat agtagagaca atgtgctgca aattcgccgc cagttgaacc taaatcgtaa
     2641 actatttgtt tgctttttat cgcaatcatc gggggggtat acatacctac taatatagca
     2701 cctgagtgtt tatcgaggac caccatgccg aaggtgaagt cttctacatc catgtagaga
     2761 ctcattaagc tagcgtactg ctctggagtt atgactttca gctgcatagc ttgcataagg
     2821 acgatcatcg gatgatcacc acacataagg gatattgctt cttttccttt gaaatctata
     2881 tgtattgctt ttttttcaat aacatctctt cgaagatgtc catcaactga ccatttgcta
     2941 tcacaagcag caaattcaaa ctctctgtat attgttgttg tcatatcaat ggcttaattc
     3001 tttttcgact atcgttaaga taccagatat acgaaaaaat cagatgttga tagttatcgt
     3061 aatagaagat acatcgatat cagttgcatt gcttatatct tgcttagctc tttcaactgc
     3121 ctcgttgagg cttagttttt tcggcgcttc tgcagagggg attggctgag atagtgttga
     3181 agtctggagt tttttccact ccagctgctc tagcgttatc cctatacggg aaaaagcttc
     3241 ctctgctgtc agataagcaa ccggataacg ctgaccgcca gttagcattt tccatgcctt
     3301 tttcactgaa atttcagcat attcctcaaa tgtaattacg tttctgtcgt cgtaatcagg
     3361 gtttttaaag catccggtga tctttgcaat cagaaagcca gttccgtgtt cgaggtcttt
     3421 caccgcccat gtttctctcc ggtttcttac tgcgatgaga tatttggcgt cattaagccg
     3481 ttctgctttt gctagccaat aacctgaacc tccctcagct ctcattatgt ttagatcttt
     3541 gcctgtaaat acgacagctg ctttattatc tttcatatag aaatcccatg ctttagtttt
     3601 ttatcgtcat gattttaatt atatacgatt aaattagggg tgtggtttta tttatgcgat
     3661 aaaaacaagc ctacaaattc tgtgcttatc tctttcttat ttttgcttat tttgtgctta
     3721 agttgttttt atttgatttt atattttttt atgtgtatct tattttgatg ttattctttt
     3781 tgggggttat atgtcgatat ctatgcagca aatagattca tgtattgaaa cgaccatcaa
     3841 tcgtctttct agcgaagctg gtacgatggt tagtaatttc tatcttgacc tgcgctctcc
     3901 cggtagacag cgcattactg aaaagctggt agagcaatca atagatcttt gcaggtctcg
     3961 tggtatctat gctgagcggg aaggtaatgg gttgcttgtg agagtagatc tgagaacgtg
     4021 ctatctgaat ccaggtcaag ctgaaatgtt taatatagct attggatata cgcgttcagt
     4081 tcacgggaat catctataag ctgtctacaa aaaggatctt cttgagatcc tttttttcta
     4141 cgcgtaatct ctagctctga aaacgaaaaa accaccctgg caggtggttt ttcgaaggtt
     4201 agttaatcct ggcagattct ctaaccgtgg taacagtctt gtgcgagaca tgccaccaaa
     4261 tctgtccttc cagtgtagcc gcactaaggg caccacttca agaactctcg atacatctct
     4321 cgcacatcct gtttaccaat ggccgctgcc agtggcgttt tgtcgtgtct ttccgggttg
     4381 gactcaagtt gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4441 acacagccca gcttggagcg aacgacctac accgaactga gataccaaca gcgtgagcta
     4501 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4561 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4621 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt gtgatgctcg tcaggggggc
     4681 ggagcctatg gaaaaacgcc agcggcgcgg ccttttgttg tcctttgcct ttttgctcac
     4741 atgttctttc cggccttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     4801 gccgactccg ctcgccgcag ccgaacgacc gagcgtagcg agtcagtgag cgaggaagcg
     4861 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggcatttc acaccgcata
     4921 tggtgctctt ttcttataat ctgcactgat gccgcatggt taagccagta tacactccgc
     4981 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gcaaggtcaa cggcctgacg
     5041 cgccctgacg ggcttgtctg ctcccggcat ccgctcacag acaagcagtg accgtctccg
     5101 ggagctgtat gtgtcagagg ttttcaccgt catccccgaa gcgtgcgagg cagctgcggt
     5161 aaagctcgtc ggcgtggtcg tgaagtgatt cacaaatatc ggcctgttca tctgcgtcca
     5221 gttcgttgag tttctccagc agcgttaatg tctggcttct gataaagcgg gccatgttaa
     5281 gggcggtttt ttcctgttta gtcactgatg cctccgtgta agggggattt ctgttcatgg
     5341 gggtaatgat accgatgaaa cgcgagagaa tgctcacgat acgggttact gatgatgaac
     5401 atgcccgttt gctggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggtct
     5461 gcctgagtga gccggttgcc cgttccggga agctgccgac actggcaccg ccgttactgc
     5521 gccagctggc tgccatcg
//
