LOCUS       NZ_CP017791            10382 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Enterococcus faecium strain E232 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP017791
VERSION     NZ_CP017791.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05933398
            Assembly: GCF_002777275.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10382)
  AUTHORS   Huang,W.
  TITLE     Complete Genome Analysis Revealed a New Mechanism of Daptomycin
            Resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10382)
  AUTHORS   Huang,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2016) Pathology, New York Medical College, 15 Dana
            Road, Valhalla, NY 10595, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3; SPAdes v. 3.5.0
            Genome Coverage                   :: about 200x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 03:34:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,124
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 46 of 128
            Pseudo Genes (incomplete)         :: 73 of 128
            Pseudo Genes (internal stop)      :: 32 of 128
            Pseudo Genes (multiple problems)  :: 19 of 128
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP017791.1.
            Bacteria available from Dr. Guiqing Wang.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="E232"
                     /isolation_source="Wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: NY"
                     /collection_date="2013-04-14"
                     /collected_by="G. Wang"
     gene            1..738
                     /locus_tag="BK413_RS16370"
                     /old_locus_tag="BK413_15440"
     CDS             1..738
                     /locus_tag="BK413_RS16370"
                     /old_locus_tag="BK413_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            766..1305
                     /locus_tag="BK413_RS16375"
                     /old_locus_tag="BK413_15445"
     CDS             766..1305
                     /locus_tag="BK413_RS16375"
                     /old_locus_tag="BK413_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            1321..1491
                     /locus_tag="BK413_RS16670"
     CDS             1321..1491
                     /locus_tag="BK413_RS16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            1969..2928
                     /locus_tag="BK413_RS16385"
                     /old_locus_tag="BK413_15450"
     CDS             1969..2928
                     /locus_tag="BK413_RS16385"
                     /old_locus_tag="BK413_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_002301399.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLFF
                     KQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCS
                     ARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITF
                     DCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFF
                     IQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
                     /name="IS30 family transposase"
     gene            complement(2892..3329)
                     /locus_tag="BK413_RS16390"
                     /old_locus_tag="BK413_15455"
     CDS             complement(2892..3329)
                     /locus_tag="BK413_RS16390"
                     /old_locus_tag="BK413_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_099745862.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLNCQIKLDRSSILT"
                     /name="helix-turn-helix domain-containing protein"
     gene            3462..3836
                     /locus_tag="BK413_RS16395"
                     /old_locus_tag="BK413_15460"
     CDS             3462..3836
                     /locus_tag="BK413_RS16395"
                     /old_locus_tag="BK413_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000713874.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            3890..6016
                     /locus_tag="BK413_RS16400"
                     /old_locus_tag="BK413_15465"
     CDS             3890..6016
                     /locus_tag="BK413_RS16400"
                     /old_locus_tag="BK413_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002289343.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            6013..6633
                     /locus_tag="BK413_RS16405"
                     /old_locus_tag="BK413_15470"
     CDS             6013..6633
                     /locus_tag="BK413_RS16405"
                     /old_locus_tag="BK413_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            6747..6884
                     /locus_tag="BK413_RS16675"
     CDS             6747..6884
                     /locus_tag="BK413_RS16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            7326..7706
                     /gene="mobC"
                     /locus_tag="BK413_RS16410"
                     /old_locus_tag="BK413_15475"
     CDS             7326..7706
                     /gene="mobC"
                     /locus_tag="BK413_RS16410"
                     /old_locus_tag="BK413_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            7688..8602
                     /locus_tag="BK413_RS16415"
                     /old_locus_tag="BK413_15480"
     CDS             7688..8602
                     /locus_tag="BK413_RS16415"
                     /old_locus_tag="BK413_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002305249.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8562..8840
                     /locus_tag="BK413_RS16985"
     CDS             8562..8840
                     /locus_tag="BK413_RS16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            8842..9531
                     /locus_tag="BK413_RS16425"
                     /old_locus_tag="BK413_15485"
     CDS             8842..9531
                     /locus_tag="BK413_RS16425"
                     /old_locus_tag="BK413_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(9652..9738)
                     /locus_tag="BK413_RS16510"
     CDS             complement(9652..9738)
                     /locus_tag="BK413_RS16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     BGC             1..8836
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..738
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7689..8599
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgagtaacg atatagttcg atataataat ggattaaatt ctgtcccttt aagaaaattt
       61 acccctatag atatggattt attttgggga atatgttcta aaatgaaaag aaaaggtaca
      121 aaagaagaaa tttttacatt taaacaaata aaagaattaa ttgattacaa tataaatgca
      181 cgtggcgacc gttttgctaa tgatttaata aaaatgtcaa aaaaaatcat tactttgtct
      241 tttatttatg aagacgaaaa aacatatgaa caattaaatc tgtttcaaaa atttaaaatc
      301 gataaagtga atgaaacatt attaataaag gtttctgaag aatttgaata tatacttaat
      361 tcaatcggaa ctaattttac tcgtttcgaa ttagaaaata tgacaaactt aaagagtagc
      421 tacgttaaag agttatatcg ccaacttatg gctcataaag acagaagatc taaaaaaggt
      481 cactggtttg taaaaataga agattttaga gctctattag caataccaga aagttatcgc
      541 atgagagata ttgataaaca aatttttgcc caagcgaaaa aagaattttt aaacgttaat
      601 tcaactcacc ctgctatttt taaaactttt gaaatcaaaa aaattaaagc ctacaaagga
      661 aataagatag gatctctgca aatttatttt gaagaaaata taccttcaat taccttagat
      721 aattggttag agaaataata cctaacaatt taggaggcaa ttatcatgca acccaaagaa
      781 acctttacaa taaaggaatt atctgaatta tttgaaataa gtaggcaagc aatctctaaa
      841 catgttgata aattagaccc gtccttatta gctaaaaatg agcgtggtta taaggttgta
      901 ttacgttctg gagtattgca acttgctagc aacttaggta accaaaggtt actatatagg
      961 ttgcatgagt atgaacaaga aaacgataaa aacgtttata tatcaagcaa aaacgtgatt
     1021 gtaaacgagt tgctagataa gttgcgtgaa gaaaatgaat ttttacgaga acaactttcc
     1081 ctcaaagagc aaaataattc taatatgcaa aaattactcg accagcaaca acagctcacc
     1141 ctacaggcaa atcaacagat cgaaaaactg caagaacagt tacaattagc ttatacggaa
     1201 gaaactccag aagatgaatc aactactatt ctttctgaag aaacagaatc gatagaaaaa
     1261 cacacaaaaa agaagaagca aaaatggtgg caactttgga aataactgga gggaacattc
     1321 atgacaagaa aaaatcttct attcggttct attttactag gaattctagg cttcagtccg
     1381 atgttattgt cagtaatcaa taaagggaat aaacatggtg aattgaaatt gaatccaaat
     1441 tattacaaca gtcctaacga agaattgttt gtaaaagaga cggataaata ataaaaaaga
     1501 ctgctcttcc gagcagccag ttaaaaaata atgagtgttt attcttgaaa aagcacgcgc
     1561 ttataaagcg ctaccaaaat tttatcatat tttataaaaa ataacaatcc agtaaaaaat
     1621 gactggtaaa aattcacaaa gaaacggaaa gaagcaacta cagaaaatca agccttaaaa
     1681 agagcttttt acctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga
     1741 ggggctactt ggcatagtgt acattatgtc aactatgcaa ttaaataact aactacttca
     1801 tattagctta ataaaataat caaaatcact aggggaaaaa atattgtata aaaaaatttt
     1861 tcatttttta aataacgccg attgtaaaat taagctagac aactaaaaaa gtcatttgtg
     1921 ctacactcaa aatagttccg accaaagaat tataaggagt gagtacaaat gacctatacc
     1981 catcttacaa cggatgaact tgtaataata gagtcttatt tcaaaatgaa tcaatctgtt
     2041 gctaaaactg cccattgctt gaatcgttca agacaaacga tccataaagt atatctgttc
     2101 ttcaagcaag gaaaatcagc cttagaatat tatcaacagt ataagaaaaa caaatcaaac
     2161 tgtggtagac gtccgcttgt tttacccgag gaacaatcag aatatattca aagaaaggtt
     2221 gttcaaggat ggacacccga tgtgattgtt ggtcgtgcag cgtttcctat tagttgttct
     2281 gctcgtacca tttatcgtat gttcaaaaag gggctatttg attcttctga cttaccgatg
     2341 aaaggcaagc gtaaaccgaa tggacatcaa gaaagacgtg gaaaacaaac tttccgccgc
     2401 tctattcatg aacgtgaaaa ggattatagc caattctcaa atgagtttgg tcaccttgaa
     2461 ggtgacacta tcgtaggtct gaaacataaa agtgctgtaa ttaccttagt tgaacgatta
     2521 tcaaaagtta tcatcacatt gaaaccgtgt ggtagacaag cgattgatat tgaaaaaaaa
     2581 ttaaatcaat ggtttgaatc tgtaccgaaa aacctattca aatccatcac ttttgattgt
     2641 ggaaaggaat tttcaaattg gaaacagatc agtaatgtca atgatattgc catttatttc
     2701 gctgatccag gaacgccgtc tcaaagaggc ctaaacgaga attctaacgg attgttacgt
     2761 agagatggtt tattgaaatc tatggatttc aattcagtag atgaattttt tattcaatct
     2821 gtcgcatcta aacgaaataa tattcctaga aaatcactgg attatcgaac acctttggaa
     2881 gtatttttga gttacgtaag tattgatgat ctgtctaact taatttgaca attaagataa
     2941 gaatagtaaa tataaaaata atttaagata tctattacta ttaatccaga tagagcaaac
     3001 caagtaaata ttccgtgtgt aaagctaaaa aaattcattt gaatcaattc aaataaacct
     3061 aatatagtaa caaatatatt aaaaataata gctgtcaata ccagtaaatt attttgttta
     3121 ttttttaaat tatcttcatt taaaatagaa tttatgttag tactataaaa ttcacaaatt
     3181 ttttttagtg tctctatatc tggtgttgtt ttgttatttt cccactttga aatagtttga
     3241 cgtgtaacat taaattttct tgccacatat tcttgagtat accctttttt cactcttaat
     3301 tctcttagct tagatcctaa atcctccatt ttttctccta tgtaaacgat tatttacata
     3361 aaattttatc ataaaaatac taacgcgcca aaaaagttct ttattttata aaacacacta
     3421 aaataaaata attctagtaa aaaagatagg agaaataagt tatgaaaaaa tttttatgtc
     3481 tttctgcttt aatgggtgtc ttattaacta gtggaggaat agttagtgct acagaagctt
     3541 taaatctaga tgtacccgaa gaccataccg ccatttacgg aggaggaatg gaagctacag
     3601 aagaaggatt ttcttctagt aaattgagat atgctgctgg cggcggagat ttcaactgtg
     3661 gggttaatgg atttaaagtt tatgcaaatt actaccatgc tagagctaaa catagtgcaa
     3721 cagctaaaaa tggacgaggc ggtcaagtac gatcagttca gaaagctgga gtaagagctt
     3781 atgcaacatg caatgccaca cttactggaa atactggttg gtggaatgtt tattaaaaaa
     3841 gagagtttaa actctctttt ttatttacat ttgaaaggaa attcaaaata tgttaaaaaa
     3901 aataaacgct gttttatcac tttttgtctg cattacaata ctattctttt ctactaatgt
     3961 tataaatggt ataaagactt ctgaagcgct atataaaaat accgctgaaa ttcatatgca
     4021 atataaagat gatatggact ttaacagtta tataaacgct ttgtatgaaa tatctacaca
     4081 agaaaatatt aatatttctc aatatagttt tactgctgtt aaccaactaa ctattcttgc
     4141 tacaaaccct aacgctaaca aacactggaa attttctaaa aaaaatatat tagcaagaaa
     4201 cgattctaaa gtccattata ctaataacaa aaattctaag taccaattaa aacttccaaa
     4261 taatttttta aatataaata tttatccttt ttctcatgtg gaaaatattg gattaagtga
     4321 aatattatat gtacaaggaa actcaaacaa attaattcct gtactgaaaa aatatggaac
     4381 acctactatt tccaatatca atcaatcaga tacttttcaa attaatacta atattgttct
     4441 tgttatctgc tatcttagta tatttattgt aattactaca attatatttg ctttttcaaa
     4501 aatgaaagaa atcactctaa aaaaaatgtt aggctataac tcttttgata ctataaaaga
     4561 ttctattaaa gagttaaacc tcttattttg ttctttgatt ataactttca ttttatctat
     4621 ttttttgagc atatattaca gtaacgaaaa ttttttccta tcatttcttc tatgtagtat
     4681 ttttcatttt ataattagtt ttttatttat catagttact ctaagtatct atcatttatc
     4741 tagtataaaa aattatctaa aaaattcacg accacttaag ttaaatcttt gtatattaaa
     4801 tatctgtcta ttcttaagca tgatccttct gttaatagct tctacaaaag taataaatat
     4861 ccataatgaa gccgaaaata attctcttaa atattgggaa agaactacta atttatataa
     4921 aacaaatatt actaatcaat taaatagaaa caacaccgta gaagaaaata actacttgaa
     4981 aaaagctagt aaatttgttt ataaaataca gaagaattat aaaactttta ttatcgcgcc
     5041 atacaattac gcaactatcc aagaaaataa taaagaattt tttattggac aaaaagttta
     5101 tccaaatttt gaagactatg ttagtcaacc tgctggtgca ggaatatgtg ttgatttaaa
     5161 ctatctaaaa tattacaatc ctatttcttt tgaagaatct actgacttaa atttaataaa
     5221 ctctgatcca ctaactactt acattctagt acctaaaaaa tataaagaat atgcaaaaat
     5281 gattgaaaaa aattatttag aagacataca gtttagactt gaagactctc ctcctcaagc
     5341 tccttataaa actcctaatt tgaaaaattt aaaaataaaa ctaatatttg ttaataataa
     5401 ccaaaagtat tttagtttta ataccttata tggaaaggca aaagataact atacaataac
     5461 tgatcccata gttagagtta ttaatccaga aataacagaa tcattatttt ggggaaatat
     5521 attgactagt gatggtggat tattttttga tcagaaacat acttcaaacc aaaacttttt
     5581 taatcaaatt aatccgtata taaaagaatt taacctagaa aatatcataa atttttctta
     5641 ttctgttttt caagaaactg gtgaattatt atctcaaaaa aaagtaaata aattaaatgc
     5701 atatctacaa tatcttttaa tattaggatt atttataata attaattttc aaatgatttt
     5761 tgttagtttt actataaatt caaaaaaaat agctttaaaa aaaatatttg gatattcacc
     5821 aataagtcaa gttttagatt tatgtttact cccttttaca atagagttat ttgctactat
     5881 gtgttttcaa ttgatatttt caaaaaacac ttacttgtat tttataattt ttatgatctt
     5941 tttaataaat ctaattactt ctattattat ttataaaaaa aatagtttta aatattttaa
     6001 ggagaatatt ttatgatatt atcagttgag aatttcaccc tgtcaattaa taaaaaaaag
     6061 atcaaacaaa attggaactt ttcaatatca gaaaaagatt ttattatagt aacaggaaaa
     6121 agtggtagtg gaaaaactac tctgttaaat aatttaagcc taatagataa acaatatacc
     6181 ggtaacttat tctattttga aaagaaagtt tctaaacatg atatacaacg tctacgaaga
     6241 aatcatatta gctatttatt tcaaaactat gctcttcttg aaaatcaaac agtacaatac
     6301 aatttcgaat tagcaaaaaa gtttaacagt aatatttcca atgatcatat ttataattta
     6361 ttaacagatt tttctttgcc aaaagatatc ttaaaagaaa aaatctttct cttaagcggt
     6421 ggagaacaac aaagaatagc attaattaga aatatgttaa aaccatttga tatattattt
     6481 gcagatgaac ctacaggaaa tttagataaa gaaaactcaa cttttataat gaactttctg
     6541 aaaaatttaa cagaaaaaga aaaaaaagcc gttgtactag taacacatga cttaaatgtt
     6601 ctaaactatg gaactaatat tatagaatta taatttttac ccgtgatgct agctgattta
     6661 aaactagcac cacgggtatt tattttatat tccatctctc ctatcccaaa atttaaataa
     6721 tcacttaata aaaaggaggc acccaagtga ttttattgaa aaaagagtat gaaaaatttt
     6781 atgatagact tatttattct ttgcaattgc caaaaaaatt aaaccgtcaa ttacaaaaga
     6841 cttatgaaac taaatcaact gaagaatggg agagttattg ctagattttt ggtagaagta
     6901 acaaaaagtg gacatattat cataatgact cgccgaaagt ctgataaaaa attggctaaa
     6961 aatattgctg aatgtttaat aaaactatca gttttagcta aatagttagt agtttacaca
     7021 aaaaagcatt tatgtttttt gttgcaaagc aacaatttca tcttcggtga aataagcgaa
     7081 actttacaaa aagtggctag catttgctag ctttttttgt attgtggagc gtggcttttg
     7141 agggggattc aagggggagc ttgcgcccct ttgacaagcg gttatttgca ccaaatacgg
     7201 aggtatttgg ctagcaaata tagagcttgc caaacccttt tagtgagctt tactcattgt
     7261 acctttttag gtacaatggt attgcgaacg atacgtccgt aggacgtact gaaagggtgg
     7321 gacttatgag cgaacaaaat caaaatttgg ctagcaattc ttctaaaaaa tatacctatc
     7381 gatccgagcc aaaacaaatc agttttcgag tgagcgaatc cgaatttgca aagctgaagc
     7441 aatcggctga agctttgcaa atgagtgtgc cggctttcgt gaaagccaag gcacaaaacg
     7501 cgcgtctggt gacaccgaaa gtggcgccag acattgccca ggcaatcgcg cgcgatttag
     7561 ccaaagctgg agggaatatc aatcaaattg ctaagtggtg caatacacac caacatgacg
     7621 ttgcttctgg tgacgctcaa cgtttatccg aaaacctaaa aatcatgcaa aaggagctac
     7681 aaaagatatg gcaacaatta aggtaagccg aaccacttct tgtagtcgtg caattaatta
     7741 cgcagaacca cgagcaacca ttaaaacagg aataaactgt gacgtcgatt atgccaaaag
     7801 tgaaatgaaa caaattcgca tgctgtatgg aaaagacgac cgtgtacagg cacacttatt
     7861 gattcaatct tttcgaccag gagaaattac cgctgaaaaa gctaatcaac taggggcaga
     7921 gtatgcaaaa aaaatagccc ccgatcacca aatagctatt tacacacata cagacaaaga
     7981 ccatattcat aatcacattg tcattaactc cgttaatctg gagactggaa aaaaatttca
     8041 agctcatggt caagcatttc taaacaaatg ttatgaccta aatgacgaga tttgtcttgc
     8101 acataactta ccaatcactg agcgaggaaa gaaaccagaa aaacgaacca tgtctgaaat
     8161 aaaattaaaa gaaaaaaatg aacctgtttg gaaagacgaa attcggattg cgatcgatca
     8221 aacaatgaaa aacaaaaaaa ccagaaccta tgaccaattt tgtaattccc tcaaaacatt
     8281 ggggattcat tgcttcaatc gcgggaaaaa tttcacttac gaattgatcc acaaaaagaa
     8341 aaaagtacga tccaacagac taggaaagga ctacgaaaag gagacgattt tacatgagct
     8401 ggacagacga gaacgaacct ataaccaaca gcgaaccaac gagctacgaa gagctcgagc
     8461 tagcattcgc gaaactgcag aagaagtacg aagagaacaa cgaactctta cgcctgaaca
     8521 accggaaatt agaccggctc gaagaaacca tgaacaacac gttgaaagag gaacagacca
     8581 ccaacaagaa ttatcgctct aaaacgttaa aagacataga aaacgaaata aagaaacaac
     8641 gtgcttttct cgttccttat acgcaagaaa ggatacaaac tacgattgat ttacaagtta
     8701 aaaaactcca tcaagagcta ttagaccagc gaaataagga acagaaaaag gtgcttattc
     8761 aacgatcatt ggattttctt gcccccttat gttgcttagt cctgacactg ttcgcatttt
     8821 ggttagtatg gggaggctaa aatggcattt tatttaccct atctactcat ttttgttagt
     8881 atttcaggaa gtatttggct catctataaa atttttcaaa caagatattc attaaaaggc
     8941 tctaaaattc gatttaagcg atttttcttt cttggctgta tattttcctt gataatcgtt
     9001 tcaagcggct tattcggcgt tttagaggga aataagcgcg tttctagaag tattttatta
     9061 ggaaatgtca ctcaaaagta tgaatcagcg cgtgataaaa agaaaaaaga gcaagcatta
     9121 gcacaaaaaa tggaagagtt taccacttgt tatgaggaaa tgaacgatat ttttgtgaat
     9181 caagaaaaac gtttgacgga taaaaacatg gaaaaactca ctcgtcttta tcaaaacttg
     9241 ccagaaaaac agcaaaaaga ggttcaagac aactatgagc gaaccaaaaa agacgtgcaa
     9301 tatgtaaaag atacaaagat agaagaagcc tgtagtgatc tatttggaga cacaaaccct
     9361 tggtttgcca gtgaagaaga aaaaaaagaa aaacaacaat ctgtcacata tgaacgctat
     9421 gaaatcttac ttcaacaagc aactaacatg caaaatccga caaaaaaaga aaccgcctta
     9481 aactatctaa aaagtgtcaa ggaatggtta gatcagcaac aacaaaattg aatttgattt
     9541 aaaatcatgt taaactgaat gtgcaaatga tcaaacatta gatacacgaa agccccagag
     9601 aggtcggatt ctctggggct ttttttcagg ttgatgtcgc ctttattgat tttacttctt
     9661 gtttcggtta ttcaaccaat aactgaataa agagacacaa actcctacaa gaagggggca
     9721 aatgaacagt tcaaacatta gatacacctc cttccagtcg caagactgtc ggataggcga
     9781 caagttatta tatcttaaaa aattaaattt gtgtggtgtt tttaagtatc acttgtttta
     9841 gggttgttag ggcttgccct gaccgcctgt aaggcgcttc tcgactagat taagggaaat
     9901 taatcaagaa ttgtcttaga acggcttaga caagctttca gagtctcaca cctcctcttt
     9961 gaacaacggt tttagcaaag gagccggagg cgactgcgca gtataatatg agagcatagc
    10021 gatcacacct ttttagcgac cgtagggagc aagttttcca gtttagaaaa attcgcgaac
    10081 ctttttggag gaaaaagtag gagtttttta ggcgggaaat tcaatgtgct cttaagatat
    10141 actgttagat atactgttag atatactgtt aataataact gttagggatt agcaacctta
    10201 tgggagagta agtattgaca taggtagtgg gggataaatt gtcacactat gggggataaa
    10261 ttgtcacact atgggggata aattgtcaca ctatggggga taaattgtca cactatgggg
    10321 gataaattgt gttttacatc ctcttatgtc tatgataaca taatttattg aggtgatttt
    10381 ta
//
