LOCUS       NZ_CP017225             1934 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Escherichia coli strain FAM21845 plasmid pFAM21845_5, complete
            sequence.
ACCESSION   NZ_CP017225
VERSION     NZ_CP017225.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05762409
            Assembly: GCF_002077195.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Marti,R., Schmid,M., Kulli,S., Schneeberger,K., Naskova,J.,
            Knochel,S., Ahrens,C.H. and Hummerjohann,J.
  TITLE     Biofilm Formation Potential of Heat-Resistant Escherichia coli Dairy
            Isolates and the Complete Genome of Multidrug-Resistant,
            Heat-Resistant Strain FAM21845
  JOURNAL   Appl Environ Microbiol 83 (15), e00628-17 (2017)
   PUBMED   28550056
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Marti,R., Schmid,M., Kulli,S., Schneeberger,K., Naskova,J.,
            Ahrens,C.H. and Hummerjohann,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2016) Institute for Food Sciences IFS, Agroscope,
            Schwarzenburgstrasse 161, Bern, Bern 3003, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v3; SPAdes v. v3.9.0
            Genome Coverage                   :: not specified
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 02:40:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,124
            CDSs (total)                      :: 5,004
            Genes (coding)                    :: 4,741
            CDSs (with protein)               :: 4,741
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 70 of 263
            Pseudo Genes (incomplete)         :: 177 of 263
            Pseudo Genes (internal stop)      :: 47 of 263
            Pseudo Genes (multiple problems)  :: 29 of 263
            Pseudo Genes (short protein)      :: 4 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP017225.1.
            Source DNA/strain available from Noam Shani, Agroscope Culture
            Collection, Schwarzenburgstrasse 161, 3003 Bern, Switzerland.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FAM21845"
                     /serovar="O68:H14"
                     /isolation_source="raw milk cheese"
                     /db_xref="taxon:562"
                     /plasmid="pFAM21845_5"
                     /geo_loc_name="Switzerland"
                     /collection_date="2010-11"
     gene            305..607
                     /locus_tag="BHT24_RS27170"
                     /old_locus_tag="BHT24_25890"
     CDS             305..607
                     /locus_tag="BHT24_RS27170"
                     /old_locus_tag="BHT24_25890"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150476.1"
                     /translation="MAGSIRARSLYLYLRDNSGDKKWHQALRTKASGLICVVEGFGSIL
                     LGVVALKATPATTAIVRGCDHLQKFDSKRQPTGDTAMITRQMSHRPAWDGVHAKP"
                     /name="hypothetical protein"
     gene            594..1580
                     /locus_tag="BHT24_RS27175"
                     /old_locus_tag="BHT24_25895"
     CDS             594..1580
                     /locus_tag="BHT24_RS27175"
                     /old_locus_tag="BHT24_25895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_016245711.1"
                     /translation="MQNPKLQNLTDYSPSDAPWDAHKSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGETLNRMNMAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPSMFTRDYVKHDRWVELWRECLRVNYDPNVDVRAVKPRKPKDGE
                     SLASATAELVRGAVAETLKYSTKPADMVADPEWFLELTKQTHKRRFVATGGALKDILKL
                     DQETDADMVIGDDIPEGDDDGSRIAFEWKTESKKYRRSPSKDKAESD"
                     /name="protein rep"
     Typing          complement(1307..1495)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.947"
                     /coverage="98.0"
                     /evalue="1.39e-59"
ORIGIN
        1 tagtgattga cgacattgct gattgaaaaa ttggctgagg tggttggatt cacgaagtga
       61 ggccaaccac ctggaagacg gtttttaatt gtctggtacc ggatacaacc agaacgaatg
      121 gagttggttc tgtcgtgatg cgaaaaagta tgggttcgca aatcacagac taaatagatt
      181 tacccaattc cattcgagtg aggttgttat gttttttgag gttcttaatc atgttgatag
      241 tgcgttgagc gttgctgttt cgctcctggc gttgtggcag tttttggtgg cgggtgggcg
      301 ttgaatggct ggttcgatcc gcgcgcgatc cttgtattta tacttaaggg ataactctgg
      361 cgataaaaaa tggcatcaag ccttgcgtac caaggcttcg gggcttattt gcgttgtaga
      421 aggttttgga agtattctgt tgggagtggt cgccttaaaa gcgacccccg caacgacggc
      481 catcgttcgg gggtgtgacc acctgcagaa attcgattcg aagcgacaac caacaggtga
      541 tacggcaatg ataacacgtc aaatgagcca tcgcccagca tgggacggtg tgcatgcaaa
      601 accctaaact ccagaacctc acggattact ccccctcaga cgcgccctgg gacgctcaca
      661 aaagcgtttc tgacgacgta ggtgggattt accttctcgc agccgaatat gagcgctacg
      721 gggcgcgtat ggcttcctgt ggcggtttac tgaggtttgg ttggtctacc ctcaaagaaa
      781 ctggagaaac tcgccttagg ctacgtgagg cgcatttctg ccgtgtccgg cactgtcctg
      841 tctgccagtg gaggcgatcc ctgatgtggc aagcccgttt ttatcagtcc ctgccgcgta
      901 ttgttgcgga ttatccagat gcacgctgga tgttcctgac cctcacagtg cgaaactgcg
      961 cgatcgggga attgggtgag acacttaacc gaatgaacat ggccttccag cgacttaagg
     1021 atcgcaaaga gtttcgacct gttcagggct ggatccgcac cacggaagtt acacgtggaa
     1081 gtgacggtag tgcgcacccg cactttcaca ctctgatgat ggttcctccc tcaatgttca
     1141 cgcgagatta cgttaaacat gaccgctggg tagagctttg gcgagagtgt ttgcgtgtga
     1201 attacgatcc gaatgttgat gttcgagcag taaaaccccg caagcctaag gacggagaat
     1261 cattggcttc tgcaactgcg gaacttgtac ggggcgcagt ggctgaaacc ctgaaatatt
     1321 ccacgaagcc ggctgacatg gttgctgatc ctgaatggtt cctcgaattg actaagcaga
     1381 cacataagcg ccgatttgtg gctaccggtg gcgcactcaa agacatactg aaactcgatc
     1441 aggaaactga tgccgatatg gtaatcgggg atgatattcc cgaaggcgat gatgatggtt
     1501 cacgcattgc ttttgagtgg aaaaccgaat ccaaaaagta ccgtcgctca ccgtcaaaag
     1561 ataaagctga atcagactga cagggtgtcg gggcgcagcc ctgaccacga cgtttttgag
     1621 aggacgccgc ttgtcggcgg tactccaaaa acacatcttg tcctattggt tctactccga
     1681 tgcaggatag ggctaacgcc gcaaagtgac gtcactcaat tactgagttc ggtttccgaa
     1741 ctcagttggg acaagatgtg tttttggtac accgccatca cgatgtcggt gccaaaaacg
     1801 attcgtcagg gctgcgcccc gacaccctgc caacacctca cgcagtcaga ttaaaatctg
     1861 ccagcgctcg ccgtttccga aatcgtcaaa ctctaaatcg tggggtcccg ctggcttgcc
     1921 agtgggttcc gatt
//
