LOCUS       NZ_CP017224             2454 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Escherichia coli strain FAM21845 plasmid pFAM21845_4, complete
            sequence.
ACCESSION   NZ_CP017224
VERSION     NZ_CP017224.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05762409
            Assembly: GCF_002077195.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Marti,R., Schmid,M., Kulli,S., Schneeberger,K., Naskova,J.,
            Knochel,S., Ahrens,C.H. and Hummerjohann,J.
  TITLE     Biofilm Formation Potential of Heat-Resistant Escherichia coli Dairy
            Isolates and the Complete Genome of Multidrug-Resistant,
            Heat-Resistant Strain FAM21845
  JOURNAL   Appl Environ Microbiol 83 (15), e00628-17 (2017)
   PUBMED   28550056
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Marti,R., Schmid,M., Kulli,S., Schneeberger,K., Naskova,J.,
            Ahrens,C.H. and Hummerjohann,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2016) Institute for Food Sciences IFS, Agroscope,
            Schwarzenburgstrasse 161, Bern, Bern 3003, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v3; SPAdes v. v3.9.0
            Genome Coverage                   :: not specified
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 02:40:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,124
            CDSs (total)                      :: 5,004
            Genes (coding)                    :: 4,741
            CDSs (with protein)               :: 4,741
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 70 of 263
            Pseudo Genes (incomplete)         :: 177 of 263
            Pseudo Genes (internal stop)      :: 47 of 263
            Pseudo Genes (multiple problems)  :: 29 of 263
            Pseudo Genes (short protein)      :: 4 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP017224.1.
            Source DNA/strain available from Noam Shani, Agroscope Culture
            Collection, Schwarzenburgstrasse 161, 3003 Bern, Switzerland.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FAM21845"
                     /serovar="O68:H14"
                     /isolation_source="raw milk cheese"
                     /db_xref="taxon:562"
                     /plasmid="pFAM21845_4"
                     /geo_loc_name="Switzerland"
                     /collection_date="2010-11"
     gene            58..297
                     /locus_tag="BHT24_RS27140"
                     /old_locus_tag="BHT24_25870"
     CDS             58..297
                     /locus_tag="BHT24_RS27140"
                     /old_locus_tag="BHT24_25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001560795.1"
                     /translation="MIKKICITVIVVFLLLVGYGAWIGSEQNQRGVSLFEVAYTYNAMN
                     PISRIGYTFMLKRNHALVERAGEVKKSIDSMSGE"
                     /name="hypothetical protein"
     gene            321..620
                     /locus_tag="BHT24_RS27145"
                     /old_locus_tag="BHT24_25875"
     CDS             321..620
                     /locus_tag="BHT24_RS27145"
                     /old_locus_tag="BHT24_25875"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016247612.1"
                     /translation="MKKILFCSLLLLSASSFAQQFHYQCEGRDLKIMLSPNAAFLDGKS
                     FEYKNGETNGETTKMTFFGPSGEVLKFLVNPVNNLYRMGLTSGATFEEINCTEI"
                     /name="hypothetical protein"
     gene            1816..2043
                     /locus_tag="BHT24_RS27160"
                     /old_locus_tag="BHT24_25880"
     CDS             1816..2043
                     /locus_tag="BHT24_RS27160"
                     /old_locus_tag="BHT24_25880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224019247.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSYMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            complement(2091..2279)
                     /locus_tag="BHT24_RS27165"
                     /old_locus_tag="BHT24_25885"
     CDS             complement(2091..2279)
                     /locus_tag="BHT24_RS27165"
                     /old_locus_tag="BHT24_25885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_081178427.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCEQMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(854..1344)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.132"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 gtgcgggcaa cggatgttat gttcaattct gttttcttca tattatctgg atagggaatg
       61 ataaaaaaaa tctgcattac tgttatcgtc gtttttttgc tgttggttgg ctatggtgct
      121 tggattggat ctgagcaaaa tcagcgagga gtatctttat ttgaggttgc atatacatac
      181 aacgcaatga atccaattag tcggatcggc tatacattta tgctgaagag aaatcatgct
      241 ttagtcgaga gggctggaga ggttaagaaa tctattgata gcatgagtgg tgagtgattt
      301 tttaaattta aaggactgat atgaaaaaga tacttttttg ttcgctgctt cttctttctg
      361 caagttcatt tgcacaacaa ttccattatc agtgtgaagg acgagattta aagataatgc
      421 tttcacctaa tgcagctttt cttgatggta aatcattcga atataagaat ggagagacga
      481 atggggaaac gacaaagatg accttctttg gtccttctgg tgaggttctc aagtttttgg
      541 ttaatcctgt caacaactta tatcgtatgg ggctgacttc aggtgctaca tttgaagaga
      601 taaattgcac tgaaatctag aaatatttta tctgattaat aagatgatct tcttgagatc
      661 gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg cagggcggtt
      721 tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt ggaggagcgc
      781 agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc aagactaact
      841 cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct ttccgggttg
      901 gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg gggttcgtgc
      961 atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga
     1021 caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg
     1081 cacgagggag ccaccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1141 ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa
     1201 cggcttcgcc gtggccttct tgcttccctg ttaagcttct tcctggaaga tgccaggaaa
     1261 tttccgcccc gccagtaagc cggtaccgct cgccgcagcc gaacgaccga gcggagcgag
     1321 tcagtgagcg aggaagcgga atatctccgg gcttatcttt ggcaccgtac gcgccatagc
     1381 tgcatttttt aatacgatgc aggatagggc agacgccgca aaatgacgtc atccgacatc
     1441 attccgccgc gttcgcccag tgacctacag tcggctctgg ggtgtcgggg ctgtgccctg
     1501 accaggcgtt ttggagtatc gccaccgtgg tggcggtgct acaaaacata tcctgtccct
     1561 tccttggcat ctctgaagtt tagcctgtat acactccgct agcgctacgt gactggttca
     1621 gggctgcgcc ccgacacccg ctaaaagcac tgacgcgcct gcggcttgcc caaccccgcg
     1681 ccgtccggga aagaatttcc cgatcgaccc gacgtttctg tttacctgtg atgttaacgt
     1741 ttggatcttt ttcccgggtt tacctatttt cttctaatga agcgttcaca gctgacgtta
     1801 atagatgggt gcttcatgtt tggtttttca ccaggagaac ttatgaacct ttgccccgat
     1861 gaacgtctgc tctttgtgcg gatgatttcc gctatgcttc gccgttcggg cggtgatgcg
     1921 ggtgctgtta tgtttgaggc gtatcgccat attgtgtccg ataccaatca agctcgtcgt
     1981 tcctacatgc tcgacctgct cgaaagcgtc aggcatgact atgtacacgg tggatataca
     2041 tagttgccat ggatacgctt ttcgtctaag tgagcgttct tttcgtctct ttattcgccg
     2101 tcctgtatgc cgaagcgtgc gctcagcttc tgataaagcg cttctgcctg ttcgtgcatt
     2161 tgctcacagt cggctgcctc gtcatcaaga tcgagcacgt cgagtttttc gagcagcagc
     2221 agcgactgcg cccggataaa ttttgccatg ttcagtgcgg ctgattcctg ttttgtcata
     2281 cggctttttc ccggttttat ggtgatcgca gactgaaatc ttacttcatt cacttgagtg
     2341 catagccatg caggtacgca gaaatcatca ggggcgtttt ttctcgatat gggcacagaa
     2401 aggtgtatgt ttcaggccgg agtcctcaga aacgccctga gggcgcctga ggtg
//
