LOCUS       NZ_CP016859            21841 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Staphylococcus aureus strain 1971.C01 plasmid p1971.C01b, complete
            sequence.
ACCESSION   NZ_CP016859
VERSION     NZ_CP016859.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04966145
            Assembly: GCF_001717685.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21841)
  AUTHORS   LaBreck,P.T., Rice,G.K., Paskey,A.C., Elassal,E.M., Cer,R.Z.,
            Law,N.N., Schlett,C.D., Bennett,J.W., Millar,E.V., Ellis,M.W.,
            Hamilton,T., Bishop-Lilly,K.A. and Merrell,D.S.
  TITLE     Conjugative Transfer of a Novel Staphylococcal Plasmid Encoding the
            Biocide Resistance Gene, qacA
  JOURNAL   Front Microbiol 9, 2664 (2018)
   PUBMED   30510541
  REMARK    Erratum:[Front Microbiol. 2020 May 14;11:877. doi:
            10.3389/fmicb.2020.00877. PMID: 32477301] Publication Status:
            Online-Only
REFERENCE   2  (bases 1 to 21841)
  AUTHORS   LaBreck,P.T., Rice,G.K., Paskey,A.C., Elassal,E.M., Cer,R.Z.,
            Law,N., Schlett,C.D., Bennett,J.W., Millar,E.V., Ellis,M.W.,
            Hamilton,T., Bishop-Lilly,K.A. and Merrell,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2017) Microbiology and Immunology, Uniformed
            Services University, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
  REMARK    Nucleotide sequence updated by submitter
REFERENCE   3  (bases 1 to 21841)
  AUTHORS   LaBreck,P.T., Schlett,C.D., Elassal,E.M., Lanier,J.B., Law,N.,
            Bennett,J.W., Millar,E.V., Ellis,M.W. and Merrell,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-AUG-2016) Department of Microbiology and Immunology,
            Uniformed Services University, 4301 Jones Bridge Road, Bethesda, MD
            20814, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 96.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001717685.1-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 00:41:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,876
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 79
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 46 of 85
            Pseudo Genes (incomplete)         :: 44 of 85
            Pseudo Genes (internal stop)      :: 17 of 85
            Pseudo Genes (multiple problems)  :: 20 of 85
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP016859.2.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..21841
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="1971.C01"
                     /isolation_source="Purulent absess from military trainee"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="p1971.C01b"
                     /geo_loc_name="USA: Ft. Benning, Georgia"
                     /lat_lon="32.3913 N 84.8229 W"
                     /collection_date="2011-09-08"
                     /collected_by="Uniformed Services University"
     gene            complement(join(21306..21841,1..109))
                     /locus_tag="A7U47_RS15325"
                     /old_locus_tag="A7U47_3024"
                     /pseudo=""
     CDS             complement(join(21306..21841,1..109))
                     /locus_tag="A7U47_RS15325"
                     /old_locus_tag="A7U47_3024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            321..449
                     /locus_tag="A7U47_RS15750"
     CDS             321..449
                     /locus_tag="A7U47_RS15750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_256262982.1"
                     /translation="MIIDDIVKWIEEQPYWQQVIAKKILDKSHIYDEDIEDFFLYL"
                     /name="hypothetical protein"
     gene            446..2638
                     /locus_tag="A7U47_RS15330"
                     /old_locus_tag="A7U47_05530"
     CDS             446..2638
                     /locus_tag="A7U47_RS15330"
                     /old_locus_tag="A7U47_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_237268897.1"
                     /translation="MKYNNLEHGNFNREKIEFRNITNTTDEHKIANWKGLDNVIGVNAI
                     KDNANLVIGPQLTLIYGENGSGKSGFTRLLNNIFISRGDTRLLSNIFKDNPSPPSANIN
                     FETNDGNIDKLYYPKDSNHPYTDHVSVFDSHSALHDLTQESELSFSPTEFKFFDKLLEI
                     VQKLKEKFEAKINDKTLDNTFINHFDKETSIKRLLLNLNTKDDFDELKIKVKLTENDME
                     NHANNNKRISELQSMQIFDKKQEYQNLISILEDVKKAIKKLNPLFSSAYLSEVKGLIEE
                     RNHYKDVSLTEGIAQFKDENIEKLGSKEWKDFIESAYKYYTTINHSVNYCIFCHQDISN
                     IKLIDKYWKYLKNESEKILAVKNNSIKKEERRFKLINTTILNKDSILSYFLKDDNIDLF
                     NSINQASNNFESLKDKVLKALNNLQWDSQIKGYNFDYNEIEKVINNLNNLIEDLDEDSV
                     KKEIEELQIKENEFNDKLKVEKLLPKIEDFINNKEWVDNASKIKINTRSITSKQNSLFS
                     KYVTDTYIETFNNECQKLNANFSAEIKQRGRKGTTLNKLTIQEKKPIDILSEGEQRAIA
                     LANFLSETSMNKNNVCIVFDDPVSSLDHKRKEIIADRLIDEAQQKQVVIFTHDLPFLLL
                     MQNKSSEKQLDCRHNTLRKIKNETGISTNSHPWISMTVKRRIGYLKEQLQNITTYYNNI
                     SSDAPEEQLEYSYKAKMWCEELRETWEKSIEELLLN"
                     /name="AAA family ATPase"
     gene            complement(3000..3095)
                     /locus_tag="A7U47_RS15480"
     CDS             complement(3000..3095)
                     /locus_tag="A7U47_RS15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000889978.1"
                     /translation="MLEILVHITTTVISGCIIALFTHWLRNRNNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            5435..5587
                     /locus_tag="A7U47_RS15340"
                     /old_locus_tag="A7U47_05540"
     CDS             5435..5587
                     /locus_tag="A7U47_RS15340"
                     /old_locus_tag="A7U47_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012818049.1"
                     /translation="MLDSAHRYTALKKIGCQYVMCQVVEKDDYTIEIWNHQISHNDFLK
                     ISPNV"
                     /name="hypothetical protein"
     gene            complement(<5560..>5688)
                     /locus_tag="A7U47_RS15345"
                     /old_locus_tag="A7U47_05545"
                     /pseudo=""
     CDS             complement(<5560..>5688)
                     /locus_tag="A7U47_RS15345"
                     /old_locus_tag="A7U47_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /name="DDE-type integrase/transposase/recombinase"
     gene            complement(5765..6937)
                     /locus_tag="A7U47_RS15350"
                     /old_locus_tag="A7U47_05550"
     CDS             complement(5765..6937)
                     /locus_tag="A7U47_RS15350"
                     /old_locus_tag="A7U47_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555828.1"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="WP_223201780.1"
                     /translation="MLTLFQFSKIIYLLLIVTFFERFSHFLIIPFLSIYLIQEFSYSSL
                     EIGIILSSSGCTALIISFILAPMLSTVNKKKIILCSIFTAFISFVIFPYIHSYYGFLFF
                     TIINTIASSLLSPTYKTLLTLYSETSRKRLVFNIRYYLINIAAALGPFISIQIQEVSSL
                     KNVCIFISFIYFINFIFFLFSSIEEKRSENPKLNYLTSFGIIKRNKAYVLLLLGSIFFV
                     FGYSQILSSIPQFFAIKSHSKATSIYGYLLSINGVIALTCQYFIYLLNKRYSHKSCIIL
                     GAITLTLGLFTFGLTENIVLLAFAMIVFTMGEMLVFTTIDIRIDEITDKYNKGAYYSLL
                     GLQNIGSLLAPVIGGLIITQFKSGMLIFSILSLITSFSIVLFYKSNKIKI"
                     /name="MFS transporter"
     gene            complement(6939..7799)
                     /locus_tag="A7U47_RS15355"
                     /old_locus_tag="A7U47_05555"
     CDS             complement(6939..7799)
                     /locus_tag="A7U47_RS15355"
                     /old_locus_tag="A7U47_05555"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="WP_237268898.1"
                     /translation="MSYQSQAKGDKLCIILIILNYQLKIFKKYFLKLQPNTKSNFFDYE
                     LSSLKELTDKNIPVPQIINKGELDNNSFLLLEFIENGHAYPESYRKLGKIVANMHKNIN
                     SLNLFGFSHNFNGGTIEFTNKWTSSWGDLFIKSRMDKLCSEIYKKRLFSISDLLLYEDI
                     RKIMLKSLYYHQSSPSLLHGDLWKGNILFQKNGDPILCDPVCLYGDREFDIGYTLALEK
                     FPLDFYQEYNNIYPLCVGYEKRIEFYKLYVFMIHLFAIGTDFYDKVKDTMYNILNNYGV
                     DFIKG"
                     /name="fructosamine kinase family protein"
     gene            complement(7834..10908)
                     /gene="mupA"
                     /locus_tag="A7U47_RS15360"
                     /old_locus_tag="A7U47_3004"
     ARG             complement(7838..10908)
                     /name="mupA"
                     /gene="mupA"
                     /gene_name="mupirocin-resistant isoleucine--tRNA ligase
                     MupA"
                     /tool="amrfinderplus"
                     /drug_class="MUPIROCIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(10935..11681)
                     /locus_tag="A7U47_RS15365"
                     /old_locus_tag="A7U47_3006"
     CDS             complement(10935..11681)
                     /locus_tag="A7U47_RS15365"
                     /old_locus_tag="A7U47_3006"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081004.1"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]; GO:1904047 - S-adenosyl-L-methionine
                     binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_001081004.1"
                     /translation="MNRLNTNYFDNFYIDWQNNEISENYVEFCQKQINNLSHIVPSLKK
                     EKRKIIDTCCGCGFHSKFLIDKGHHVISFDKSEEMLNFAYKKNGILGKRQSLDEEFPEN
                     HVDLILNLNTSIGYKKNDLKNLLRNSYTSLKKGGILIIDLMNPAFFYKNFEEQMIFNFK
                     DYEVNQTIKINKIYNSIVSTWDVYKNNEYSKDLSRELKFKLYTTNDIICSSLYYNFTLS
                     NISFYGDMKGNEFHENSPRLIVKITK"
                     /name="class I SAM-dependent methyltransferase"
     gene            <12430..12690
                     /locus_tag="A7U47_RS15710"
                     /pseudo=""
     CDS             <12430..12690
                     /locus_tag="A7U47_RS15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822410.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /name="IS6 family transposase"
     gene            complement(12704..14623)
                     /locus_tag="A7U47_RS15380"
                     /old_locus_tag="A7U47_05570"
     CDS             complement(12704..14623)
                     /locus_tag="A7U47_RS15380"
                     /old_locus_tag="A7U47_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_105165752.1"
                     /translation="MDKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTVKPESMILTPQNAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDLDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDMNNPHAHVLLTQRPFNSDGTWGKKTKTRTR
                     YDENGDAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEEQ
                     GLEKIAEIPLTREEYRLEKNEQKRCEKEGIEYQPVTYYGQKNEEIRRYNRDEVSEIYSD
                     KEKEEAQNAIRLLVQNANDRVSKNEQSYSVLIDRYQRDVGYSEAKETIKNTFDSASTFG
                     RIIQNDLLKNEIKRIQLKFYADNYDTNYQFRKELESKGYSQQEFINTVKSNFKALLNEK
                     NKLESREEKRKAIYQSAKDVYLTEIEKNNLIVQHMYPKSHHRFSHDEKAFIVDEAQKGN
                     FIKATNVQYLFKQNNNIPTDVLLEDTYKKASKDIFFSERQLQHLEKDSFEYNVELQFIR
                     EKEKEIESYKEHIDDDLRIDIGDRNFEKVKDQTPNEKVQLLIKLEKYNHIDQEEVIGKH
                     INQVDRNDNDNHLEHEYEQSNHINLPTINASDFLMYYANEIEKQNDINHKKKKKQERNN
                     HMRR"
                     /name="MobA/MobL family protein"
     gene            14827..15108
                     /locus_tag="A7U47_RS15385"
                     /old_locus_tag="A7U47_05575"
     CDS             14827..15108
                     /locus_tag="A7U47_RS15385"
                     /old_locus_tag="A7U47_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002489259.1"
                     /translation="MSKIKQTDITKLSQLEQQIEQLKKQKKKQEEQLSNKIGDYFLNKL
                     DLNMINSSDELYKIMDKVIEEFNKYIEQNSSDENNENLTENKINQNSK"
                     /name="hypothetical protein"
     gene            15113..15352
                     /locus_tag="A7U47_RS15390"
                     /old_locus_tag="A7U47_05580"
     CDS             15113..15352
                     /locus_tag="A7U47_RS15390"
                     /old_locus_tag="A7U47_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_002489265.1"
                     /translation="MNDFKNLKKTNAAIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKIYANYINTNKPNKFKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(15577..16512)
                     /locus_tag="A7U47_RS15395"
                     /old_locus_tag="A7U47_05585"
     CDS             complement(15577..16512)
                     /locus_tag="A7U47_RS15395"
                     /old_locus_tag="A7U47_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_002489267.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSIQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELISFNLLTQERVGLN
                     KPNRLYLLKPNYDIKATHSKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTENNDTD
                     YIKTENNDTNDLNDNKLTFPSNHTNHSNHNNLNFNNEALKFQLLEELPQSIQNYLSNFE
                     VAEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNESVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            16953..17720
                     /locus_tag="A7U47_RS15400"
                     /old_locus_tag="A7U47_05590"
     CDS             16953..17720
                     /locus_tag="A7U47_RS15400"
                     /old_locus_tag="A7U47_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002489261.1"
                     /translation="MRTIKMVADELGVTKQTIVNNAKSLNIAFEKENGINYIDDKDYLK
                     IIEKITKKESTIQNKESVKKELFGENTAKAEKNIYNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILALESNKKIQKLENQLEEERQLN
                     YSFDTATNNRQNINSQEATFTEESQNINQQQTKRQPTEVNYKDIAEEKKNDSTNEGELT
                     KEDKFVEETKSDPDDNREVEPPKKGFWSRFFGN"
                     /name="DUF536 domain-containing protein"
     gene            complement(18044..18604)
                     /locus_tag="A7U47_RS15410"
                     /old_locus_tag="A7U47_3021"
     CDS             complement(18044..18604)
                     /locus_tag="A7U47_RS15410"
                     /old_locus_tag="A7U47_3021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(18809..20353)
                     /gene="qac"
                     /locus_tag="A7U47_RS15415"
                     /old_locus_tag="A7U47_3022"
     CDS             complement(18809..20353)
                     /gene="qac"
                     /locus_tag="A7U47_RS15415"
                     /old_locus_tag="A7U47_3022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_002505088.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIVSSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFVIIAIVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTMFAMTSVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGTASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            20530..21096
                     /gene="qacR"
                     /locus_tag="A7U47_RS15420"
                     /old_locus_tag="A7U47_3023"
     ARG             20531..21093
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.47"
                     /coverage="100.0"
     ARG             complement(18813..20353)
                     /name="qacB"
                     /gene="qacB"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacB"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.42"
                     /coverage="100.0"
     ARG             complement(7835..10908)
                     /name="Staphylococcus aureus mupA conferring resistance to
                     mupirocin"
                     /gene="Staphylococcus aureus mupA conferring resistance to
                     mupirocin"
                     /gene_name="antibiotic-resistant isoleucyl-tRNA synthetase
                     (ileS)"
                     /tool="rgi"
                     /drug_class="mupirocin-like antibiotic"
                     /identity="99.61"
                     /coverage="100.0"
     Typing          complement(15578..16512)
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.304"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(12708..14623)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccgactgttt ctctgtaaag attttctcca caccaaacgt ctttaaatta tccaattgtc
       61 tttctaaatt ttgatctatc gatgatactc tcgcataacc tataatcatt catcttcact
      121 ccataattaa tatacaccct aatcatacgt tctaattgcg ataatttcaa caacttattt
      181 tgtatgatta gggtgtacct ttatcagcct aatttttttg ataatatata cacaataaaa
      241 taataaaaat atatcttcta cttttaaagt attagtataa taaagttgca atctatattt
      301 ttataaaagg agaatgaaaa gtgattattg atgatatagt aaaatggatt gaagagcaac
      361 cttactggca acaagttata gctaaaaaaa tactagacaa gagtcacatt tatgatgaag
      421 atatagaaga tttttttcta tatttatgaa atataacaac cttgaacatg gaaattttaa
      481 tagagaaaaa atagaattcc gaaatattac taatactact gatgaacata aaatagcaaa
      541 ttggaaaggt ttagataatg ttattggtgt aaatgccatt aaagataatg ctaatttagt
      601 tattggtcct cagttaacac taatctatgg tgaaaatggt agtggtaaat cagggtttac
      661 tcgtttactt aataatattt ttatttcaag aggagatact agattacttt ccaatatatt
      721 caaggataat ccgtcgcctc cttcagctaa tattaacttt gaaactaatg atggtaatat
      781 agataaacta tattatccaa aagattcgaa ccatccttat acagaccatg tatcggtatt
      841 tgattcacat agtgctttac atgatttaac ccaagaatct gaactatcat tttcaccaac
      901 agaatttaaa ttttttgata aattattaga aattgttcaa aaattaaaag aaaaatttga
      961 agctaaaatc aatgataaaa ccttagacaa tacatttata aaccattttg ataaagaaac
     1021 ttctatcaaa agattgttac taaatttaaa tacaaaggat gattttgatg aattaaaaat
     1081 aaaagtaaaa ttaactgaaa atgatatgga aaatcatgct aataataata agcgaatatc
     1141 agaattacaa tcaatgcaaa tatttgataa aaaacaagag taccaaaatt taatatcaat
     1201 tcttgaagat gttaaaaaag caataaaaaa actcaaccca ctttttagtt ctgcttattt
     1261 aagtgaagtt aaaggtttga tagaagagag aaatcattac aaagacgtat ctttaactga
     1321 agggatagct caatttaaag atgaaaacat agaaaaatta ggttcaaaag aatggaaaga
     1381 ttttatagaa tcagcctata aatattatac aactataaat catagtgtta attactgtat
     1441 tttttgtcac caagatatta gtaatataaa actaatagat aaatattgga aatatttaaa
     1501 aaacgagtcc gaaaaaatat tagccgttaa aaataattct atcaagaagg aagaaagacg
     1561 ctttaaactt ataaatacaa ctattttaaa taaagattct atattaagct actttctaaa
     1621 agatgacaat atagatttat ttaattctat taatcaagct tcaaataatt ttgaaagtct
     1681 caaagataag gtccttaaag ctctcaataa tttacaatgg gattcacaaa ttaaaggtta
     1741 caattttgat tataatgaaa ttgaaaaagt tataaacaat ttaaataatt tgattgaaga
     1801 ccttgatgaa gacagtgtaa aaaaggaaat tgaagaacta caaattaaag agaatgaatt
     1861 taatgataaa ttaaaagtgg aaaaattact tcctaaaata gaagacttta taaacaacaa
     1921 agagtgggta gataatgcta gcaaaataaa aattaacaca cgttctatca cttcaaaaca
     1981 aaacagttta ttttctaaat acgtaactga cacatatatt gagacattca ataatgaatg
     2041 tcaaaaatta aatgctaact tttcagctga aatcaaacaa agggggagaa aaggtacaac
     2101 cttaaataag ttaaccatac aagaaaagaa accaatagac atactgagcg agggagaaca
     2161 acgggctatt gctttagcaa attttttgtc tgaaacctca atgaacaaaa ataatgtttg
     2221 tattgtattt gacgaccctg tttcttctct tgatcacaaa agaaaagaaa taattgctga
     2281 tcgattaatt gatgaagcac aacaaaagca agtagtaatt ttcactcatg acctaccttt
     2341 tttactatta atgcaaaata agtcttcaga aaaacaactt gattgtcgtc ataatacact
     2401 tagaaaaatt aaaaatgaaa caggtatttc tacaaactca catccttgga taagtatgac
     2461 tgttaaaaga agaattggtt atctaaaaga acaacttcaa aatatcacaa cttattataa
     2521 caatatctct tctgatgctc cggaagaaca gttagaatat agttataagg caaaaatgtg
     2581 gtgtgaagaa ttaagggaaa cttgggaaaa aagtatagaa gaattattat taaattgatc
     2641 tatcgaaaga tttaatccag caattcaaac gtctagacta aaaaaagctc catttaatag
     2701 agaattatat gaagaagttg aaaaaggtat gacagaatct tcaaaatggg ttcatgatag
     2761 gtcatctgcc ctaggcgaag aaacaccgag ccccgaacaa ttgaaggaat atattgatga
     2821 tttggaaaaa ttcacaaaaa aactaaaaaa attaataaag tagtagtagt atatatatat
     2881 agttttgcat ttctctacta accagtgtta taatttagtt aacaaggaaa ctagcataga
     2941 tgcaccaatc ccctcactac ggcaatagtg aggggatttt taggtgtggc tatagtcgcc
     3001 tatttattat tgcgattacg tagccaatgc gtaaataacg caataataca accactgatg
     3061 actgtggtcg tgatgtgaac aaggatttct agcatagatt agcacctcct ctctgcgtct
     3121 gaattgacgc ctgagagata ggcgacctac atattatatc atctccgcat atacgatgat
     3181 agataacttc cgataagtca ttttatgtaa tgatagtgag attcagatcc atttctatga
     3241 tatctagagg caaaagagat actttataag tatatttcaa gtttattata gtaattaaac
     3301 acatcactat aagtaaataa atttactagt ttactaatga ctattgcatt tttaatagaa
     3361 ttattgattt tatacttact ttccaataat actttttagc accttttaaa atctaatatg
     3421 cttttacttg atcctatata ctcttaaatt tgcgtcattt catttaacag ctttcctaat
     3481 aattttcgct ttgaacatta gaaaattgat gatgatttat gttttcttta actttaattt
     3541 ataggagaaa aatcatgatt ctgttaattt atgaaatctt attattttta ataatttcat
     3601 tctcttattt tttattcaaa ctatttttat ggagattcat tttggaatct tagcttcaat
     3661 attcggaatg tttacaacaa atttgtttat gtgtgacatg cttctatata aaagtccaga
     3721 atacaatgac aagaaaatct ttaagatttt tataaactta atcaatctcg taataattat
     3781 tattagctta ataatactca atttattaac tgtaaaataa gtacagaatt agaattgaaa
     3841 ttaatgtatt aacgttgatc tttgaagctc ttaacaattt tcaaacttat aaaattgtcg
     3901 actaaatcgc tcataccata cgatattcat cttaaaattt agttaagtat actcacaaca
     3961 ttattaaaat ttcttatcat aaatgtgcgc tctacttttg tttttaccct tgtctaacat
     4021 atggaaaaat gacgcataaa ttctgaaaaa attaatcaaa atattccatt atttcgttcg
     4081 attatttaac aagaacctct gaaaaaatgc agaacttatt aatgacttaa aatgaatttt
     4141 atatcaaaaa tgtatattaa gtttcggtgt aattacttaa ataaaaacat aaacttttaa
     4201 atttagataa cactgaatat ggtaatttaa ttcaaaaaat gataaagaaa aaaacacatt
     4261 caattacagc aattgggcac ttcgaaaaca aaattatctc ttaaaaaata gggaacttta
     4321 gctaaaaaag ctagcatttg ttattgaaaa gtcgaaaaaa aagtatgggt acaaaatggg
     4381 tacagataca atatgtaggg gtacattttg ggtacaaaca caaaatttag gcaccaaaag
     4441 aaaaactctg aaacctttga aatgtctggc ttcgccagac ctacctttgt aaaagatggc
     4501 aaatttccct tatgctctta attcatgatt tgggttcaaa tcaaaatttt ggtgttttaa
     4561 aagaaaaatg tctccgaaaa aactaaaaaa caaagttggt ttttaaatga ttttatgcca
     4621 aattttaatt tacatgtaac tgataagcat ctcaaaaatt tgtctctggt tcatcaaaaa
     4681 agtttagact tgttttttta attagctcat atttgtgttc tcagatcctt taaatgattt
     4741 tgaattaaat atacataaaa tacgcctaaa tgttctaaaa gtatctttaa ataacaaata
     4801 aggtatttaa aattttaata ggacttgtga taaagtttgt tgaaaaggaa ctctatatgt
     4861 tttggaattt ttgtatgctt gttaaatatc ttagtccctt tagcaacagt ttttgaagaa
     4921 gagacaacaa atttatttgc taaaataaaa gataaaaata aagaagagaa tcattaaaaa
     4981 aaggagtgtt tttagatatc atcttgtatt gttgtccaac tattattatc tattttgatg
     5041 cttgttttta tacatcaaca aataaatcaa agccatttag atttttggtt tattatttac
     5101 ttcttatttt ttatagttgt ggttttgttt tggaaacctt cgaaagacaa aaagtagacc
     5161 acacatttat gcagagaaaa tttattgtgc gttgagaaga acccttaact aaacttgtag
     5221 acgaatgtcg gcataacgtg agctattaag ccaaccgttc gacaagtttt gggtttgtta
     5281 agggttccga ggctcaacgt caataaaaca attggaataa aaaactgata taattatttt
     5341 aattaactgg ggagattttt tgaaatataa agttattaga gaggaaaaac aaagaaaccc
     5401 tattatcgtt acaaagtaca atagaggtta tcttgtgtta gatagtgcac ataggtatac
     5461 agctttgaaa aagataggtt gtcaatatgt aatgtgtcaa gttgtagaaa aagatgatta
     5521 tacaatagaa atatggaatc atcaaatttc ccataacgac tttttaaaaa taagtcctaa
     5581 tgtgtgacag tctgtatcgt gcgattgata cagatagcac cattttcctt tgattttgat
     5641 atatgtctca ttaatacgcc gtttacaata ggctttttta ggctttttca tcaaaataat
     5701 tcccccacta aagttgagtt ttaaggttca actttagggg gggaattcaa aaaatataca
     5761 gcaattatat ttttatttta ttacttttat aaaataatac aatgctgaaa gatgtaatta
     5821 aagaaaggat gctaaaaatt aacattccag atttaaattg agtaataatt aaaccaccaa
     5881 ttactggagc taataatgaa ccgatgtttt gtaaacctaa caaagagtaa taagctcctt
     5941 tattatactt atcggtaatt tcgtctattc taatatctat agtagtaaat actaacattt
     6001 cccccatagt gaatacaatc atagcaaaag caagtaatac aatattttct gttaagccaa
     6061 aagtaaataa tcctaaagtt aaagtaattg ctcctaatat aatgcatgat ttatgagaat
     6121 atcgcttgtt taataaataa ataaagtatt gacaagttaa agctatcaca ccatttatag
     6181 acagtaagta accataaata gaagtagctt tagagtgcga ttttatggca aagaattgag
     6241 gtatagaact tagaatttga gaataaccaa atacaaagaa tatacttcct aaaagcaata
     6301 atacataagc tttatttctt ttgataatac caaaagaggt aagatagttt aatttaggat
     6361 tttcgcttct tttttcttct atagaagaaa aaagaaaaaa gataaaatta ataaagtaga
     6421 taaaagaaat aaagatgcat acatttttta aagaagatac ttcttgaatt tgtatagaaa
     6481 taaaaggtcc taatgcagca gctatgttta tgagatagta ccttatatta aatactaatc
     6541 gttttcttga tgtttcagag tataaagtta ataaggtttt atatgtagga gagagtaaac
     6601 tacttgctat agtattaatt atagtaaaga ataaaaaacc ataataacta tgtatgtatg
     6661 gaaatattac aaagctgata aaagctgtaa aaatactaca tagtataatt ttcttcttat
     6721 ttactgtact taacatcgga gctaatataa aactgatgat taatgctgta cacccggaag
     6781 aagataaaat aatccctatc tctaaactag aatatgaaaa ttcctgaatt aaataaatag
     6841 ataaaaaagg tattatgagg aaatgtgaaa atcgttcgaa aaaagtgact attagaagta
     6901 gataaatgat tttagaaaat tgaaacaaag tcaacaccct atccttttat aaaatcaacc
     6961 ccgtaattat ttaaaatatt atacatggta tctttaactt tatcataaaa atctgtacct
     7021 atagcaaata aatgaatcat aaacacatat aacttgtaaa attctattcg cttttcatat
     7081 ccaacacaca acggatatat attattatat tcttgataaa aatctaatgg aaatttttct
     7141 aatgctagag tatatccaat atcaaattct ctatctccat ataaacagac tggatcacat
     7201 agaataggat ctccattttt ttgaaaaaga atatttcctt tccaaagatc accatgaagt
     7261 aatgatggtg acgattggtg ataatataag ctcttcaaca taatttttcg tatatcttca
     7321 tataataaaa gatccgaaat tgaaaataaa cgtttcttgt atatttcaga acatagctta
     7381 tccatcctac ttttaataaa taaatccccc caagaagaag tccatttatt agtgaattca
     7441 atcgttcctc cattgaaatt atgactaaag ccaaatagat ttagtgaatt tatattttta
     7501 tgcatgtttg caactatttt acctagtttt ctatagcttt caggataagc atggccattt
     7561 tcaatgaact ctaacagtaa aaaagaatta ttgtctaatt ctcctttatt aataatttga
     7621 ggaacaggaa tatttttatc agttaattct tttaaactgg ataactcata atcaaaaaaa
     7681 tttgatttag tattaggttg tagctttaaa aaatattttt tgaatatttt taattggtaa
     7741 tttagaataa tcaaaattat acatagttta tcaccttttg cttggctttg ataactcatg
     7801 gccactctac taaaatagag tggccatgtt tgtttaatat aataaggaaa tttttatttc
     7861 tttattatta aaatgaatag tattttcata ttcatctata acatttaagt gtatattttt
     7921 aatcagcaaa ttttctttaa acatattttt attggttttt attgattcta ataattcccc
     7981 agttacaccg atataaatat caatacgttg atttattggt aaatttttct tttttcttaa
     8041 ttgttgaatt aatctaatga gctctctagc atgtccttcg cgaattaatt caggagataa
     8101 atttttatct aataatataa cgcagtcatt atcttcactg agttgataac tatccttagg
     8161 taaggtttta attataaaat catccttagt taactcagca tcagaagata aacttttgaa
     8221 gttattagac tcaattaaac tttttttatc atactctgat agggagtcta tcaatgtttg
     8281 aatatttttc gttttgttcc ctagttttgg tcccacagaa gaaaaattca atttaagctc
     8341 ataggtaata tagtcattaa tattatcagt taattcaatg ttttttatat ttaattcgtc
     8401 ttttattatt gaatagtaat taggtaaaaa acttaagttt aattgactat taggtttaat
     8461 caccattttc gataaaggtt gcttaatttt taaatctgca tttttgcgag cctgtctaga
     8521 taattctaca atttttataa cggtatgcat ttcatcttcc aatgcttgat taataaaact
     8581 ttctttatat tgtggataat cttgtaaatg cacacttttt ccagtcaaat tataatggat
     8641 tttttcagat ataaatggaa caaatggagc cactagtctt gataaagttg ttaaaatttc
     8701 ataaagcgta ttgtacgcat cttttttatc gttagaaatt ccttgttccc aaaatcgtcc
     8761 tctcgatctc cgaatatacc aattacttat tgtattggta tattcgttaa ttaatctggc
     8821 tgcggaagtg aaatcgtaat tatttacata aatattactt tcttttatta aggtgtttaa
     8881 gcgagaaaga gcccaattat ctaatgtatt tctacttttt gtaatttcat tattaggatt
     8941 atagtgatct attttattat acatattata aaatttataa atattatcaa gcgtatctat
     9001 aaatttcgat ttggtctgag ctactatatt ttctgagaat cttttgttat tccatggagc
     9061 actatccgaa attaaggccc atcttaaaga atcggctccg tacttattaa ttaattcagt
     9121 tggattaata acgtttcctt tacttttaga cattttttta ccattactgt ctagaatatg
     9181 tcctaaagat aaagcacgtt tataagaaga ttttcctttt agaatagtag aaattactag
     9241 taaactgtaa aaccagcctc tcgtttgatc aactccttct gcaataaaat cagctggaaa
     9301 gtgttgatta aaaattttct ggttatcaaa aggataatga tgctgagcaa acggcataga
     9361 gccgctatca aaccaaacat cgattacttc ttctactcga gacattttcc cattacactt
     9421 agggcaacta agagtgatat tatcaacata aggtctatgc aactcaatat cttcatcaat
     9481 tttattgatg gaattatttt gtaaatcctt aatactactt ggtgcgtatt cgtgattaca
     9541 atcattgcaa atccatacat ttaatggtgt tccccaatat ctatttctac caatgttcca
     9601 atcaaccata ttttctaaga aatttcccat tctcccttcc ttaatatgag aaggaaacca
     9661 ctctatatta ttattattgt taataatttc attcttaaaa ttagttgttt taataaacca
     9721 accttccatc gcataatata tcaaaggatt accacatctc caacaatgag gataattatg
     9781 ctcatatttt tgttttttat ataaaagttg ttttttggat aataatttta tgatttctat
     9841 atcactattt ttagctttat taccaactaa ttcagggaac ctatcattat atactccttc
     9901 tcttgttata acatttaaga aatccaaatc acgctctaaa accaattggt agtcatcttc
     9961 cccatgagct ggtgctatat gaacaatacc agttccttct gagttagtaa caaattctcc
    10021 atcaacaacg taatatgcat taactaaacc gtcgctttca aaaggaggaa tgtattttaa
    10081 attaattaaa ttacttcctg aaaaggtatc aataatttcg tatttttcag ttattataga
    10141 attaattaga tctgtagcta agatataata ctcattttct acccgaattt ttgaataatt
    10201 aagatcttta tttatagcta atgctacatt tgcaggcaaa gtccagggag tggtagtcca
    10261 acttaagaaa taagtatctt tactatttgt aagttgaaat ttaacaacag ctgataaatc
    10321 tttaacgttt ttatagcctt gcgctacttc atgagaactt aaagcggttt gatcatgtgt
    10381 acaataagga gtcaccttat gtcccttata taatagtcct tttttatgga atgtagataa
    10441 tatattccat acactttcaa tataattatt ctcaagagtt atataggggg agtccatgtc
    10501 aacccagtat cctaaatctt tagaaaaatc ccgccattct ttttcataat taaatacact
    10561 ttttttacat tcatttataa aattttctat tccatacttt tcaatgtctt gttttccttt
    10621 aattccaatt tttttttcaa cctctaattc aactggtaag ccatgggtat cccatcctgc
    10681 ttttctttct acataaaaac cttgcatagt ttttaatctt gcaactaaat ccttgattac
    10741 tcttccaaga acatggccag catgaggaag gccatttgca gttggggggc cgtcataaaa
    10801 aacaaaactt tcctgtcctt ttctattgtc aattgatttt ttaaatatct tttgagtatt
    10861 ccaaaatgct gatatttcat tctgggtgtt taaatatttc tttgtcaaaa cttttcagcc
    10921 tcctagagta tcacttattt cgttattttt actattagtc ttggtgaatt ttcatgaaat
    10981 tcatttcctt tcatgtctcc atagaaagat atattggata aagtaaagtt ataatacaaa
    11041 ctactgcaaa ttatatcatt agtagtatat aatttaaatt ttaattctct tgaaaggtct
    11101 ttgctatatt cgttattttt ataaacatcc catgttgaaa ctatactatt ataaatttta
    11161 tttattttaa tagtttgatt aacttcgtaa tctttgaagt tgaaaatcat ttgttcttca
    11221 aaatttttat agaaaaaggc ggggttcatc aaatctataa ttaaaatacc accttttttt
    11281 aaggaggtat aactatttct taacaaattt tttaaatcat tttttttata tccgatagaa
    11341 gtatttaaat taagaattaa gtcgacgtga ttttctggga attcttcatc taaagattgt
    11401 cttttaccta aaatgccatt ttttttatat gcaaagttca acatttcctc agatttgtca
    11461 aagctaataa catgatgacc tttatctatc aaaaatttag aatgaaaacc acagccacaa
    11521 caagtatcga tgatttttct tttctctttt tttaatgaag gaacaatgtg acttaagtta
    11581 ttaatttgct tttgacaaaa ttcaacataa ttttctgata tttcattgtt ctgccaatca
    11641 atataaaagt tatcaaagta atttgtattt agccgattca ttattgctct ctccattcct
    11701 gtatactttt actaataata gtgcagaccc tttttacatc attgatagat aaacgattat
    11761 gtaagggtaa tgtaactaaa cctttagaaa cctcctctac aattggtaat aagttttttg
    11821 attggttctg ttgcaaagtt aaaattatat gcacatctat acaaaactat aaaagcaaat
    11881 attgttttaa cagtaaagag ttttgttagt attaattatt tttcgttttt cagtataaat
    11941 attacatgat ttttaacttt gcaacagaac catttttttt atgttcatac ttagaattta
    12001 ttttatccat ttcaggtgta taatttatta actggtcttc tatattgttg taagcattgt
    12061 ttatagcgtc tatttggtta tttttttgtt attattgttc ttcactaact ggttatacct
    12121 tgattctatt tgacttactg tatatttcaa attgtataac attaaaagat atgattctaa
    12181 ttcgtttata ttcatatttc gtaatacttt attatcgtcg atatttttat atttactttt
    12241 aaaatcatta acactttgta tgttgaaatt attcattata ttgttccctt cattttaatt
    12301 attctattaa ttacaataat acaataaata gtgggttttt ttgctcttat tttaaatttt
    12361 cttttttaat atttaaatat ttatagcgca aaagtttcaa taaattcaat ttaactcgtg
    12421 acgtaacttg agtttgataa acctcgaatg ataattatca accaggcact ttaaacgaag
    12481 ataacaatat ctaaactgat taaaatttta aacttaatcc taactgtcat tgtatctcta
    12541 aatatcttaa taacctcatt gattaatgat taccatcata ttacagtgag aaaaacaagg
    12601 tatcaaagta tcaatatggc aaagaatact ttaacaggta ttaaatatat ttatgtccta
    12661 tataaaaaga accgaatgaa ctttgtataa ttcccaccac ctattatctt ctcatatggt
    12721 tatttctctc ttgttttttc tttttcttat gattaatatc attttgcttt tcaatttcat
    12781 tcgcatagta cataagaaag tcactggcat ttatcgttgg taaattaatg tgattagatt
    12841 gttcatattc atgttcaaga tggttgtcat tatcatttct atccacttga ttaatatgtt
    12901 ttccaataac ttcttcttga tcgatatgat tatatttctc aagtttaatg agtaattgaa
    12961 ctttctcatt aggtgtttgg tctttgactt tttcaaagtt tctatcgcct atatctattc
    13021 ttaaatcatc atcaatatgt tctttataac tttcaatttc tttctctttt tctctaatga
    13081 attgcaactc aacattatat tcaaaactat ctttttctaa gtgttgaagc tgtctttcac
    13141 taaagaaaat atccttactt gcttttttat aagtatcttc cagcaataca tctgtaggaa
    13201 tattgttatt ttgtttgaat agatattgaa cattagtcgc ttttataaag ttaccttttt
    13261 gcgcttcatc aacgataaat gctttctcat catgactaaa acgatgatga gatttagggt
    13321 acatgtgttg aacaattagg ttattttttt cgatttcagt tagatataca tccttcgctg
    13381 attgataaat tgctttacgt ttttcttctc tactttctaa cttattcttt tcattgagta
    13441 acgctttaaa gttagactta actgtattaa taaactcttg ttgactatat cctttgcttt
    13501 caagttcttt tctaaattga taattagtat cgtaattgtc agcatagaat tttaattgaa
    13561 ttctcttaat ttcatttttg agtaagtcat tttgaattat acgaccaaag gtagatgcac
    13621 tatcaaacgt atttttaatg gtttctttcg cttctgaata gccaacgtca cgttgatatc
    13681 tatcaattaa aacagaataa ctctgttcat ttttgcttac tcgatcatta gcgttttgaa
    13741 caagtaatct aattgcattt tgtgcttctt ctttctcttt atcactatat atttctgata
    13801 cttcgtcacg attatatcgt ctaatctctt catttttctg tccgtaatac gtaacgggtt
    13861 ggtactcgat accctctttt tcacagcgtt tttgttcatt tttttctagt cggtattctt
    13921 ctctagtcag tggaatttcg gcgatttttt ctaagccttg ttcttcaaaa gaacgactat
    13981 catatcgtct taaactttgt tctctttctg aatattgatt taattttaac tcccaatgct
    14041 tacgaacttc tttaaaatca aagtcggcaa agcgttcttg ttttctaact ttattaccat
    14101 ttttatttaa aatcgcatcc ccgttttcat catagcgtgt tctcgttttt gtcttttttc
    14161 cccaagtacc gtctgaatta aacggtcttt gggtcaataa cacatgcgcg tgtgggttgt
    14221 tcatatcatc cctatgaatt gaaatatcag ccaccatacc tttatctaca aattcacttt
    14281 tgataaaatc ttttgttaat tctgtttgta cttctctatc taggtcatta ggcaaactta
    14341 acaagacttc ttttgctaat ctagggtttt tcgttttcgc attattcttt tcgactttat
    14401 cgacttcatt ccataaacgc tgtctatctt tagtccattc gggtgcattt tgaggagtta
    14461 aaatcatgct ttcaggctta acagtatgat ttctaaactt aattttttca tctgtacgtt
    14521 cactatataa cgcttcatca gaacgatacg atgctgacgc aacgatattt ttagttgctc
    14581 gattaatgtt agtcactctc aacataaagt aacctttatc catgatttta cctcctctct
    14641 ttttttgaat ttaaacccct gtgggaacta taacgatcaa cccgtagggt tgcccacttt
    14701 tatgccaatt tttaatttgc atgtaaatgg gcagtgtctt aagaactggt tatattatac
    14761 catgaatgaa cgacttgacc aatgaattat gatatacttt atatagataa actgaaggag
    14821 ttttttatgt ctaaaatcaa acaaacagat attacaaaat taagtcaatt agaacaacaa
    14881 attgaacaat tgaaaaagca aaagaaaaaa caagaagaac aactatctaa taaaattggt
    14941 gattattttt taaacaaact tgatttaaat atgatcaact ctagtgatga attgtataaa
    15001 atcatggata aagtcattga agaatttaat aaatatattg aacaaaatag cagcgatgag
    15061 aacaacgaaa atttaacaga aaacaaaatt aaccagaata gtaagtgata cgatgaatga
    15121 ttttaaaaat ctcaaaaaaa caaatgctgc aattgaaaaa gctgaattaa gaaaacatag
    15181 attaaaaaat ttagatcgca aagaaagagc gcatagactt attcgaaaag gcgccatgct
    15241 tgaaaagtat tttgaatgtg agcatttaag tcctgatgaa acagaagaat tgttaaagat
    15301 ttatgccaac tacattaata cgaataagcc aaataaattt aaaaagaagt aatttatcat
    15361 gcttacaaat acctgagttc cactaaaaag aaaaaattgc tgatagtagt aacaatgaat
    15421 cataattgtt tataaaaatg ggactaaaat cgaaaagttt tagtcccact tacttaatta
    15481 agtacatttc attgaaaata aagttagcct acagttaaga aaacatctaa ttaaagatat
    15541 tctctaattg aatcattgtt ggcttatgaa gttgtattat tgattaaata aagaagtgat
    15601 gggtaaatga tcacgtcgtt tattaagcgt atgtacttct gcaaattcag atttaagaga
    15661 tttcattaag tacccttgca ttgcttcaac gctttcattt ttttgaatta atgtagcttt
    15721 aaagcgctta agaacattaa caatttcaaa ttccatatct tctaataaat aatatgcatc
    15781 aatagaattg ttgaaggatg ttttggcttt taataacaca gatttaataa gtttaatttc
    15841 agctacttca aagttactta gatagttttg aatactttgc ggtagttctt cgagtaattg
    15901 gaattttaaa gcttcattat taaagtttaa attgttgtga tttgaatgat ttgtatgatt
    15961 actaggaaaa gttagtttat tatcattcaa atcattcgta tcattattct cagtcttaat
    16021 ataatcagta tcattattct cagtatcatt agactgtgaa tttggaagtt cttgaccact
    16081 taaattcaca gttctagaac ttccaaattc attgttctgg aactgtgaat ttggaagttc
    16141 cttgctatga gtggctttaa tgtcataatt aggttttaat aggtataatc tatttggttt
    16201 attaaggcca acacgttctt gagttagtaa attaaaactt attaactctt tttttaattt
    16261 tataattttt tgttctgcat aacctaagta ttcacacaaa tgtttattac tgaaaataaa
    16321 gtaaatgtta ttatttttat ctacccattc attttggatg gataattcaa agcgatcttt
    16381 taataacgca tacataattt tggcttccgc cgacattttt ttaaaatatg catcttcgaa
    16441 taaatattta ggtagttgat aaaacctttc agtttttact tgtgataaat tgtatttttc
    16501 aaaattgggc ataataaaaa cctcatttcc atttttagga ttgaggtaat aaaacaattt
    16561 ttacgtaagt tatatgtttt gtgcttgcac ttagatgatt agatagatat aatacaagta
    16621 tcaaaaaata tataagcaaa cgagtgcgaa tcgttttatt accgagagtc aatgctacgc
    16681 caatagcatt gactctttta atatttataa agcaatatta acacttttaa gaaagtatat
    16741 aaagagtttt aaatgtagtt aaaagcacat tgccatggca aaatgtgtag ttcattgttt
    16801 tagtacctaa ttaagcttcg aaaacatatt atagggagct aattaggtgc ataggtagta
    16861 aataggtagt aaatttaggc ggtaaatgat gaaataaact tcattaggtg gtaaaattta
    16921 ggtactaaat taggtaccta agggtgaatg ttatgagaac tattaaaatg gttgctgatg
    16981 aattaggtgt aactaaacaa actattgtta ataatgctaa aagtttaaat atagcttttg
    17041 aaaaagaaaa tgggattaat tatattgatg ataaggatta tttgaaaatt atagaaaaaa
    17101 ttactaagaa agagagtaca atccaaaata aagaatcagt aaaaaaagaa ttgtttggtg
    17161 aaaatactgc aaaagcagaa aagaatatat ataataattc aaatagttct gagacattaa
    17221 aaacaaaagt aaatgaatta gaaaagcaaa tagaaatctt tgaaactaga gctaaaaatg
    17281 atgaaaaata tattgagaat ttaactaagc aattagatca acaaaatagc aatgttaata
    17341 cattaaataa attgttggag aatcaacaaa tattagcttt agaaagcaat aaaaaaattc
    17401 aaaaactaga aaaccaatta gaagaagaaa gacagcttaa ttattctttt gatacagcga
    17461 cgaataatag acaaaatatt aattcgcaag aagcaacttt tactgaagaa tcgcaaaata
    17521 tcaaccaaca acaaacaaag cgtcaaccaa cagaagtaaa ttacaaggat atagccgaag
    17581 agaaaaagaa cgacagtaca aatgaaggtg aattaacaaa agaggataag ttcgttgaag
    17641 aaacgaagag tgatcctgat gataataggg aggtagaacc acctaaaaag ggattttgga
    17701 gtcgattctt cggtaattaa aaatgaatgt aaaaggtgta tcaagggggt tataaatctt
    17761 tgatacacct tttatagtga tggaacttct gataatcatc agttatgtta aactctaaca
    17821 aaaactaaat tatcagaaga attatctagg gtattaaaca taggtaaatc aattttctat
    17881 cgccaatagt ataaacatca tccatattaa tctgaagata gctacataat tctttaacag
    17941 catagccttt atttgtataa ggcgccataa actctattaa atatggtttg ttttgaggtt
    18001 tctatgttta atcatttaat ttttcattat ataaatcgaa taattataaa aagtcatcat
    18061 actttttatt ttttaaaaat atttgaaaat ttaatagtaa tccaattata agatcaaata
    18121 tcattatcat tataatcaag gcaactataa tcactgtgac actcgttaca taacaagcta
    18181 gtaatctcca gataaaattg acattttcac caattccata acttaacata ttacaaagta
    18241 gtaaaagaat aataatacct atactgtaaa ttaaaattgt cagtgtatat tttttaataa
    18301 ctctattagg aaacaatagt tcatctatta ggttcttttt caccagtatc tctcgttcaa
    18361 ttctatcttt gtcacagatt ttgattttac tattttttat tttttcctct aataaaataa
    18421 cctcgaacga tctgctaatt tttaataaat atgcaggaaa agctaatgtt aagcataatg
    18481 taattaattc atttacattg ttcatgtaac ttataaaata gatgaaagtt gctactagtg
    18541 ctcctaaaaa tatagttata ttaagttcta catcaaatat tttaaaaata tctgctctat
    18601 tcatcagtta atcatccttt caaggtttat taatactaat aaattattag atataggtat
    18661 atcatattat taatttaaga aattgtcttt tataatttta cttaataata aaaaagtaga
    18721 accatttaaa attaatggtt ctacttttta actattacta attttaaaaa taaactaatc
    18781 tacaatatct aaaaatatat gtttagtact atttttgttt agttatatca agtgatttgg
    18841 gaattaacaa atagacaact attgaaatga taatcatgat aatcccacct actaaagcag
    18901 ttgctacaaa agcatcatta aatgatgtta cagcctcgtt tgcaagctgt tttatccccg
    18961 tagctttagc tacttcgaca gcgcccacta cagattcttc agctacatga gctaaatctc
    19021 caactatacc ttttgatgaa aaagatgaaa tatctaaaaa tacacgataa agcatagaag
    19081 ataggctacc aagtactgct acaccaaaaa catttccaag gtcatacata gactcttcaa
    19141 cagcagctgc attacctgct tttgatgtag gtgtttctaa cattattaga gcagatgcaa
    19201 ctgctagtga agccgtacca gctccaacta aaattaatgc taaagccatt gtagaatatg
    19261 ataatggatg accaaagaaa tacataataa acatgccaat ggctgcaatt ccaattccgg
    19321 aaggtaacac tattttcggt ccaaatcgcg ccgctaatcc gggtgcaatt ggtgcaaaca
    19381 ccatatctcc tattgccata ggtaataggt ataagccagc tttaaaagga gaaagttctt
    19441 ccacaacctg taaccattgt gaagctaata acaaaacaga tgtcattgca aacattgtca
    19501 taaatgcagc aattgtacca gctgaaaatg atctcttttt aaaaagtctt acgtctaaca
    19561 ttggatcaga acttgataaa ttacgtttaa caaatatcac tatcatggta attgctaata
    19621 ctattacaac ccatggaata atatctgcta gtccttcttt tgaaaattct ttgatactcc
    19681 ataccagtcc aatcatgcct gcaattgata aaattgtaga aggaatatcc cacgagtgag
    19741 acttttcttt tgataactta gactctggta ataaaaataa acctgctaca attgctatta
    19801 tcacaaacgg tacattaatt aaaaatgccg agtgccatga aaattgctca agtaaagctc
    19861 ctccgataat tggtccaaaa acagcaccta tcgatgaaac gattgaccat acagctaatg
    19921 cagtggccct ttctttaggg ttttcaaaaa ttactcttat cattgaaaga gtagttggca
    19981 ttattaaagc acctgcaata ccaagtaaaa atcgaatagc tattacgaac tctgcacttt
    20041 ctgcgaaaaa tatagctaat gaaacgaggc caaataaagc aaatccagtt aataatgctt
    20101 tttttcttcc ccatttatca gcaaaggcac tcaatggaat tataaagcca gctaaaacaa
    20161 gagagtatat atcaactatc cataactgtt gggtaccaga aggctctaac tctcttacta
    20221 attccggtaa agccataatt aatattgtca tatccattgt aacaacaaac aaactaacag
    20281 ctaatactac tagtgcagtc catctttttt ttgatgtcat catatcagta gtttttgtaa
    20341 aaaatgaaat catttaagtt ccctccaatc cttatagacc gatcgatcgg tctataagga
    20401 ttataatcta tttttttaca tattacaaca aaaaatcttg cgaaatttaa atggctgtat
    20461 tgtgttatac aattattctt tgaatttttg tgctatcatt gatagtactt aatactcatt
    20521 aaaggcgtga tgaacttgaa agataaaata ctaggtgtcg caaaggaatt atttataaaa
    20581 aatggatata atgccactac tactggagaa attgttaaat tatcagaaag tagtaaaggg
    20641 aatctttatt atcactttaa aacaaaagaa aatctatttt tagaaatttt aaatatagaa
    20701 gaatctaaat ggcaagaaca gtggaaaaag gaacaaatca aatgtaaaac taatagagaa
    20761 aaattttatt tatataatga actatcttta acaaccgaat actattatcc acttcaaaac
    20821 gcaataattg aattttgcac tgaatattat aaaactaata gcattaatga aaaaatgaat
    20881 aaattagaaa acaaatatat agatgcttat catgtaattt ttaaggaggg taatttaaat
    20941 ggcgaatggt gtattaatga tgttaatgct gttagtaaaa tagcagcaaa cgctgttaat
    21001 ggaattgtta cctttacaca tgaacaaaat attaatgaaa gaattaaact tatgaacaag
    21061 ttctctcaaa tttttttaaa tggacttagt aaataattat atatatattc ttgaaatacc
    21121 aataaatatt atttctaaat tacgttcatt attgataaaa aatttaaata gtattactac
    21181 acaaaaagag agtgcgaatt cgcactctct tttttcttgt ataattatta aaagatcatt
    21241 tcaataatat aatatgagtt ttgagactct gtgagagcct cttatttgca ttgacgaccc
    21301 atttatcaag atgatccctt atcatgctta attctataaa ctgtctgtct tgtgatatta
    21361 acttctttcg caatcttact aattgctttc ccttcttcta acatttccac cacgcgatga
    21421 taaataatac gtttctgtgg gtctttggca tttggtgaat aaagtaaggg gcgcccttta
    21481 tatacgcctt tttccttcgc aacttgaatt ccttgcgctt gtcgacgttt actttcattt
    21541 ctctcttgtt ctgaaaccat tgctaaaatt tgaacgatta aatctttcat aaacttatcc
    21601 agtaatggat tgccaatcac ttcattcatc ataggtaagc tggtaatcat taactgaacg
    21661 tctttctctt ttaaataact cacagtttca ataatttcat cataatcacg tcccaaacga
    21721 tcaatagatt ccacaacgaa tcgatctccc attctgacaa aattgagtgc ttcttgaaat
    21781 acaggtctat tttccactga ctttcccgac tgtttctctg taaagatttt ctccacacca
    21841 a
//
