LOCUS       NZ_CP016009             8556 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain NGF1 plasmid pNGF1_pCol_let_like, complete
            sequence.
ACCESSION   NZ_CP016009
VERSION     NZ_CP016009.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05194365
            Assembly: GCF_001660585.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8556)
  AUTHORS   Karrenbelt,M.A.P., Bues,J. and Silver,P.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2016) Systems Biology, Harvard University, 200
            Longwood Avenue, Boston, MA 02115, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2016
            Assembly Method                   :: SPAdes v. v3.7.1
            Assembly Name                     :: NGF1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 22.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001660585.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 01:43:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,884
            CDSs (total)                      :: 4,771
            Genes (coding)                    :: 4,613
            CDSs (with protein)               :: 4,613
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 79 of 158
            Pseudo Genes (incomplete)         :: 100 of 158
            Pseudo Genes (internal stop)      :: 42 of 158
            Pseudo Genes (multiple problems)  :: 48 of 158
            Pseudo Genes (short protein)      :: 1 of 158
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP016009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="NGF1"
                     /isolation_source="gut flora of female BALB/c mice (Charles
                     River Laboratories)"
                     /host="Mus musculus"
                     /db_xref="taxon:562"
                     /plasmid="pNGF1_pCol_let_like"
                     /geo_loc_name="USA: Massachusetts, Boston"
                     /collection_date="2014"
     gene            complement(join(8516..8556,1..490))
                     /locus_tag="A9Z04_RS24735"
                     /old_locus_tag="A9Z04_24450"
     CDS             complement(join(8516..8556,1..490))
                     /locus_tag="A9Z04_RS24735"
                     /old_locus_tag="A9Z04_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_058813529.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTISTMDTHWQTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTE
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<528..1226)
                     /locus_tag="A9Z04_RS28295"
                     /pseudo=""
     CDS             complement(<528..1226)
                     /locus_tag="A9Z04_RS28295"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1168..1491)
                     /gene="mobC"
                     /locus_tag="A9Z04_RS24740"
                     /old_locus_tag="A9Z04_24455"
     CDS             complement(1168..1491)
                     /gene="mobC"
                     /locus_tag="A9Z04_RS24740"
                     /old_locus_tag="A9Z04_24455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_032286519.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQMAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="mobC"
     gene            1556..1747
                     /locus_tag="A9Z04_RS24745"
                     /old_locus_tag="A9Z04_24460"
     CDS             1556..1747
                     /locus_tag="A9Z04_RS24745"
                     /old_locus_tag="A9Z04_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_042634456.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENH"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2417..2575
                     /locus_tag="A9Z04_RS28300"
     CDS             2417..2575
                     /locus_tag="A9Z04_RS28300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(2772..2912)
                     /gene="crl"
                     /locus_tag="A9Z04_RS26495"
     CDS             complement(2772..2912)
                     /gene="crl"
                     /locus_tag="A9Z04_RS26495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R system lysis protein"
                     /protein_id="WP_010892547.1"
                     /translation="MKMILSGGMLVLMALLSGCQANYIRDVQGGTVAPSSSSKLTGIAV
                     Q"
                     /name="crl"
     gene            3032..3556
                     /gene="cui"
                     /locus_tag="A9Z04_RS24750"
                     /old_locus_tag="A9Z04_24465"
     CDS             3032..3556
                     /gene="cui"
                     /locus_tag="A9Z04_RS24750"
                     /old_locus_tag="A9Z04_24465"
                     /inference="COORDINATES: protein motif:HMM:NF015795.4"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity protein Cui"
                     /protein_id="WP_021534853.1"
                     /translation="MRKEHTDNKDLNRTLFLITFAGITPLIIIFITYTSNPKFYLISII
                     FDNTQNIPSIISAYNPVMTKVMDIYGKSAPLLALIAFTLQLRDRKLETIANREKLITAS
                     IFSPFFYAFYAYFFLWNNFELTTAGRTVRWMSDNDFTLFIFYACLYFCSFFMTYALCYI
                     PVVSYKLWKER"
                     /name="cui"
     gene            complement(3584..5473)
                     /gene="cya"
                     /locus_tag="A9Z04_RS27570"
                     /old_locus_tag="A9Z04_24470"
     CDS             complement(3584..5473)
                     /gene="cya"
                     /locus_tag="A9Z04_RS27570"
                     /old_locus_tag="A9Z04_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin Y"
                     /protein_id="WP_106372970.1"
                     /translation="MVCFKSQRGMNMPGFNYGGYGDGTGWSSESGGPAPGGGMHGNSGG
                     QRGDNANSSNSVSQQISAIQNDQKLKQKVVNMLIAARKMNPEAKMILGSIAPSGVMQVT
                     IEGITSTQAKQLGLGGLVMGYNASGVIGAVGEIDTGHRLNASGASTPGSETSVESFVNG
                     QKPAGEWHAVAKDSWTGAGPVNVGLVNNAIKSVRIIKKGYVTGVLLPEEVMNKAEYKAM
                     RQAFDSLPLAKQGEAVRQIVAAWSLAYQDFPVNLKKDMGRVTERVVDAVNLALILNQIS
                     SGMSASQKDVDAANRIINETVKAINDVNLKIAEKKKQQVPLLSLMKQKQKEVEELKKVF
                     KNHSYHRIRDAQRAYDDARNKNDLLVSDINALQAQVSGLTARKQQAEKNKAAAEKAKAD
                     AKAKAEAEKAAAEAKAKAEAEKARKEAEAKANNEKAVLTKASEIIISVGDKVGEYLGDK
                     YKALSREIAGNIKNFQGKTIRSYDEAMASVNKLMANPDLKINAADRDVIVNAWKAFDAE
                     DMGNKFAALGKTFKAADYVMKANNVREKSIEGYQTGNWGPLMREVESWVVSGIASAVAL
                     AIFSATLGAYLLAVGASAAVVGIIGIIIASFIGALIDDKFIDRLNNEIIRPAY"
                     /name="cya"
     gene            5699..6136
                     /locus_tag="A9Z04_RS24760"
                     /old_locus_tag="A9Z04_24475"
     CDS             5699..6136
                     /locus_tag="A9Z04_RS24760"
                     /old_locus_tag="A9Z04_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021536733.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKQNETQERHTVGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRHQESEREQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     gene            6217..6645
                     /locus_tag="A9Z04_RS24765"
                     /old_locus_tag="A9Z04_24480"
     CDS             6217..6645
                     /locus_tag="A9Z04_RS24765"
                     /old_locus_tag="A9Z04_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_021536732.1"
                     /translation="MKRSGGTRYLYLISLITVAAALTACTPKGSVEQYTRHYVYASDDR
                     SDPNFYTNKADTTRMMIPFFQQFREMGEKDKAAGVSAETAQQRIKEFHSEKFLQSLRST
                     TTFAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(8276..8509)
                     /locus_tag="A9Z04_RS24780"
                     /old_locus_tag="A9Z04_24495"
     CDS             complement(8276..8509)
                     /locus_tag="A9Z04_RS24780"
                     /old_locus_tag="A9Z04_24495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021534848.1"
                     /translation="MTELEKHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1833..1914)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          1943..2489
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.536"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(7..1178)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.629"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(8194..8555)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="82.812"
                     /coverage="99.0"
                     /evalue="7.94e-52"
ORIGIN
        1 cctccgtcca gttcgtttcg gctttcatcc ccttcggcaa cacaagaaaa cgcccgttgc
       61 tgtcctcgtg ataacgcacg ccccatgtct tcgcgttcag cttctccaga ctctctttct
      121 gctcgcggag aatattcatg tttgcattta tcttcatgcc ctgataccac agcagactcc
      181 cggtcgtggc aaacatcagc actgagacga taatcagcca cagccagcgt ttcacgctca
      241 ttttcagtac ggtttgccag tgtgtatcca tcgtgctgat ggtcgttttc tgatatctct
      301 ccagcagttc tttcagggct gttgtatggt cgttgatagc gttcctgatt aaagattcgt
      361 ttgaggtcag ttccctgttt acagattctt cgagcttcct gaactcgtcg ttcagcattt
      421 tctctgtaga ttcggctctc tctttcagct ttttctcgaa ctccgcgccc gtctgtaaaa
      481 gattgctcat aaaacgctcc tttcagtctg atattcttcc ctccgttcgg gtctgcaatg
      541 ctgatactgc ttcgcgtcac cctgaccact tcaatccctg cctccgtgag cgcctgaatc
      601 acatcctgac ggccttttat ctccccgaca tgataaagag cgtctatccc gcgtgtgacg
      661 ctctctgcaa gcgcctgttt cgtttccggc aggttatcag ggagagtcag tatccggcgg
      721 ttctccgggg cgttggggtc atgcagcccg taatggtgat taaccagcgt ctgccaggca
      781 tcaattctcg gcctgtctgc ccggtcgtag tacggctgga ggcgttttcc gctctgtagc
      841 tccatgttcg gaatgacaaa attcagctca agccgtccct tgtcctggtg ctccacccac
      901 aggatgctgt actgattttt ttcgagaccg ggcatcagta cacgctcaaa gctcgccatc
      961 actttttcac gtcctcccgg cggcagctcc ttctccgcga acgacagaac acctgacgtg
     1021 tatttcttcg caaatggcgt ggcatcgatg agttcccgga cttcttccgg attaccctga
     1081 agcaccgttg ccccttcccg gttacgctcc cttcccagca ggtaatcaac cggaccactg
     1141 ccaccacttt ttcccctggc atgaaactta actatcatcc cgcgtcccct gttccctgac
     1201 ggccagacgc agccggcgca gctcatcccc gatggccatc agtgcggcca ccacctgaac
     1261 ccggtcaccg gaagaccact gcccgctgtt caccttcctc gctgtctgat tcaggttatt
     1321 cccgatggcg gccagctgac gcagtaacgg cggtgccagt gtcggcagct tcccggaacg
     1381 ggcaaccggc tcacccaggc agacccgccg catccatacc gccatttgtt taccctcaca
     1441 gcgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctcgcg
     1501 tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac
     1561 aaaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct
     1621 gctggagaag ctcaacgaac tggacgcgga tgaacaggca gatatctgtg aatcgcttca
     1681 cgaccacgcc gatgagcttt accgcagctg cctcgcacgc ttcggggatg acggtgaaaa
     1741 ccactgacac atgcagctcc cggagacgta cacaactggt ctgtgaatgg atgccgagag
     1801 cagacaagcc cgtcagggcg cgtcagcagg ttttagcggg tgtcggggcg cagccctgac
     1861 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcgacatc agtgcggatt
     1921 gtatgaaaag tgcatcatag ccgatgtgaa atgccgcaca gatgcgtaag gagaaaatgc
     1981 aacttccgac actcttccgc ttcctcgctc actgactcgc tacgctcggt cgttcggctg
     2041 cggcgagcgg taccggcgca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2101 aaggccggaa agaacatgtg agcaaaaagg caaaggccaa cgaaaggccg cgccgctggc
     2161 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2221 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2281 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2341 aagcgtggcg ctttctcata gctcacgctg ttggtatctc ggcttggtgt aggtcgttcg
     2401 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2461 taactatcgt cttgagtcca acccggtaag gcacgcctta acgccactgg cagcagccac
     2521 tggtaaccgg attagcagag cgatgatggc acaaacggtg ctacagagtt cttgaagtag
     2581 tggcccgact acggctacac tgaaaggaca gtatttggta tctgcgctct gctgaagcca
     2641 gttaccttgg ttaagcagtt ccccaactga cctaaccttc gatcaaaccg cctccccagg
     2701 cggttttttc gtttacaggc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2761 ttttaacttt tctactgaac cgcgatcccc gtcagtttag aagaggagga tggtgcgaca
     2821 gtccctccct gaacatcgcg gatgtaatta gcttggcagc ctgatagcaa agccatcaag
     2881 acaagcatcc ctcctgataa tatcattttc atatattccc tcattaacac atacccctaa
     2941 tcttaggatg accattactt gtacagcatg atatgcttat acacatctac aactaaatta
     3001 ataataatga aaagcttgga gagttatacc aatgagaaaa gagcacactg acaacaaaga
     3061 tttaaacagg acgctttttt tgattacttt tgcaggcata acaccattaa tcattatttt
     3121 tataacttat acatcaaacc ccaagttcta tttaattagc atcatttttg ataacacaca
     3181 aaatatacct tcaataattt ccgcatacaa tcctgtgatg acaaaggtga tggatatcta
     3241 cgggaaaagc gctcccttac tggcacttat tgcttttact ctccaactcc gcgacagaaa
     3301 gctagagaca atagccaaca gagaaaaact aattactgcc agcatattta gtccattttt
     3361 ttatgctttt tatgcatatt tctttctgtg gaataatttc gaattaacca ctgcaggtag
     3421 gactgtcaga tggatgtccg ataatgattt tactttgttt attttttacg cctgcctcta
     3481 tttttgctct ttctttatga cttatgctct gtgttatatt ccggttgtgt catataaact
     3541 ctggaaagaa aggtagtctt cttgactacc tttcgactct aggttaatac gcagggcgaa
     3601 taatttcgtt atttaacctg tctataaatt tgtcatcaat caatgctcca ataaaactgg
     3661 ctataataat accgataatc cctacgactg cagctgatgc ccccacagca agcaggtaag
     3721 ctccaagggt ggcagaaaaa atagcaagag ccacagcaga agcaatacca ctgacaaccc
     3781 aggactcgac ctcccgcatt aacggccccc agtttccggt ctgatatccc tctatactct
     3841 tctcccggac gttatttgcc ttcatcacat aatctgcggc tttaaatgtc ttacccagcg
     3901 cggcaaactt attccccata tcctctgcat caaatgcttt ccaggcattc acaatgacat
     3961 ccctgtctgc agcattaatt ttaagatcag gattagccat cagtttattg acagaagcca
     4021 tcgcctcatc ataactacgg atcgtcttac cttgaaagtt tttgatatta cctgctatct
     4081 cacgagaaag agctttatat ttatcgccaa gatattctcc gaccttatca cccacactaa
     4141 tgataatttc actggctttt gtcagaacag ctttctcgtt atttgctttt gcttcagctt
     4201 cctttctggc tttttcagct tcagcttttg cttttgcttc agcagccgct ttttcagctt
     4261 cagctttcgc ctttgcatca gcttttgctt tttctgctgc cgccttgttc ttttctgcct
     4321 gctgtttcct tgcagtcagt ccggaaacct gtgcctgcag cgcattaata tcactgacca
     4381 gtaaatcatt tttgttccgg gcatcatcat atgctctctg agcatcacga atacggtgat
     4441 aagaatggtt cttaaatact ttttttagtt cttcaacctc tttctgtttt tgtttcataa
     4501 ggcttaataa tggtacctgt tgtttcttct tctccgctat tttaagattt acgtcgttaa
     4561 tggctttcac ggtctcatta ataatccggt tcgccgcatc aacatccttc tgtgaggccg
     4621 acattccgga tgatatctgg ttgagaatga gcgccaaatt aaccgcatcc accacccgtt
     4681 cagtaactcg ccccatatct tttttcagat ttaccgggaa atcctgatat gccagactcc
     4741 aggctgcaac aatctgtctg acagcctctc cctgttttgc cagtggaagg gaatcgaaag
     4801 cctgccgcat tgctttgtac tctgctttat tcatcacctc ttcaggcaag agaacaccag
     4861 tcacatagcc ttttttgata atccggacac ttttgatggc gttattaacc agccctacat
     4921 tcactggacc agcccctgtc cagctgtcct tcgccacagc atgccattct ccggcgggtt
     4981 tctgtccgtt cacaaaactc tccacagaag tttcgcttcc gggagtactt gctcctgatg
     5041 cattaaggcg gtgaccggtg tctatttcgc ccacagcccc aataacacca gaagcgttat
     5101 atcccatgac cagccctccc aaaccaagct gcttggcctg agtggaggtt atcccctcaa
     5161 tggtgacctg cataacacca gaaggagcaa tacttcccag aatcattttt gcttcaggat
     5221 tcatttttct cgcagcaatc agcatattca ccactttctg tttaagtttc tgatcattct
     5281 gaatggcaga aatctgctga ctcacagaat tgctactgtt tgcgttatct ccgcgctggc
     5341 ctccactatt tccatgcata ccaccgcctg gagctggacc accactttca gaactccagc
     5401 cagtaccgtc accataccca ccgtaattaa accccggcat attcattcct ctttgacttt
     5461 taaaacaaac cattaaaaac aaatactgta tacataacca ctggttttat gtacagtaca
     5521 aaagctacgc cgctgcattt tccctgtcaa gactgtggac ttctattttt gtgaaaacga
     5581 tcaaaaaaac agtctctcac accacgcgct attcccgccg gataccacaa aaaccggcgc
     5641 aaacattacc gttctcagac ctcattatgt tttactgaaa ccatgagatg agacacctat
     5701 gggacactgt aactttatgg catggcacac actccgggac gcactgaaaa tgactggaag
     5761 atcgcgttca cagttttacc gtgatatgcg ctcaggtctt gtcagttacc gtaccagcca
     5821 ggacggacga agggagtttg aaaccagtga actgatccgg gcatacggcg aattaaagca
     5881 aaatgagaca caagaaaggc acactgtggg acaggcagaa aatccacatg atcagcagac
     5941 agaacgcatt ctccgggaac tgaatgagct gaaacaatgc ctgacgctga tgcttgagga
     6001 taaacaggca caggatatgg atcgcaggca ccaggaatca gaacgggaac agttacagaa
     6061 tgagatagcc cagctcaggc aggcactgga acaggaaaag aaacggggat tctggtccag
     6121 actcttcggt cactgaactc ggtcagaggc tgatgataag atagtattcg gataataact
     6181 caccgagaat aaatacttta aggtaaggag acacgtatga aacgttctgg tggcacacgc
     6241 tatctgtatc ttatatccct gataactgtg gctgcagccc tcacggcctg taccccgaaa
     6301 ggcagtgtgg aacaatatac ccgacattat gtgtacgctt ccgatgaccg ttctgaccct
     6361 aacttctaca ccaacaaggc cgacaccaca cggatgatga ttccgttctt tcagcagttc
     6421 agggagatgg gggagaaaga caaagcagcc ggagtatcag cagaaacggc acagcaacgc
     6481 ataaaagaat tccactcaga aaaatttctt cagtctctca gaagcacaac cacctttgcg
     6541 ggcagaaaat atacgaacag tgatatgcca tcaccggaaa aaatgaagct actggctgac
     6601 acgatttctg cggtttatct cgatggctac gagggaagac agtaaatgga tttaccataa
     6661 tcccttaatt gtacgcacca ctgaaatgcg ttcagcgcga tcacggcagc agacaggtaa
     6721 aaatgacaac aaaccacccg aaaaaccgcc gcgatcgcac ctgataaatt ttaacctcat
     6781 gcatatctat gcagccatgc gaatcacgaa cgaattgcct gcctgatgta actgaaacgg
     6841 gggttttttc atattttcgg caggttcaac tcctgccaga tggaagcatc aggagggaaa
     6901 tattactgta cgtgacagaa ctcatcaccg tacacacagg tatcatgctc cagaaaaagg
     6961 aaacaattgc ctgataccag aaatcgcgtt acaacacttc tgagcgcgtt tctgacagtc
     7021 tccatcaact gagctgaatg ttcgtcacac aacatcgaaa cgtgctcacc atccgcgacc
     7081 aacgcccaat gaaactctct cttccggaag cgcccaattc gaaaaataac cggtagacct
     7141 cctgtctgtc tcaggattct ggcacagtaa tcagggaaca taactttaca gcgccccctg
     7201 acctgcatcg aaactaaaaa ccgccggaag aagtttttta aaatggcgaa ccagacatcc
     7261 acaactctcg acacagagcc cttgctcagc taaaaattta caggcgcgtt tctggtgaat
     7321 tgttcgttga tgtagaatgg gttagtcgca tcaccgccag aaacgcgctc ataagacaga
     7381 acttcactgc ctttatagtt actactcaaa acaatataaa acagagagaa aaagacaaaa
     7441 aaaagtccct caaggagcag ggactgttaa ttgcgtctga atataagaaa aaattagaag
     7501 aattcacttt cagcttaaga ataacggtac agagcaataa caatcaacaa aaatgatcag
     7561 attttacttt ctgttatttt gtcttttttg taaaaaacaa aaaaaaataa ccaggaaaat
     7621 tcctggcatc aatattcttg tttatagttt gttttatggt taatcttatt actctccaga
     7681 ggtgaccaac accaggatat ttttttgctc tgtatcactc agcccctccg tcaggtgggg
     7741 cttccttttt tcacacccat gaaaaaagcc ctcacggcag gaaatcagtg agggcgtaag
     7801 gtaaggactg aatcgatgga tagtatgtct agtcctgact tttgcatatc agaatataat
     7861 tcgcagactt caaatatgca aaaaaaaata tgcaaccaca tgtaagaact caatattaac
     7921 acatgagaga acgcgcatct ccgacccctt ccagattcgc tgtgtccaca tcggtattac
     7981 aaacatatcg gagatacgca attatatttt ttacataacc cgcgcattcc cgcaagttca
     8041 ttttcagaaa aatacctgtc ctctttacaa aaaaaacagc aacaacctgt acaaaaatca
     8101 ataaaccgca agaacgactg gttatattac caataggcag cctgacattt ctggaaaata
     8161 ttctgtttac gactcaaccg gactttcagt caccatccca gcgaagggcc atccagcttc
     8221 cgctcccgtt ccagagataa ctcatgttcg cgttcctgca tcagccgttc gtcccttatc
     8281 tcctgttctc gctgtataac tggctcaagc gctctgtccg ctcgctcaag tgctgcacct
     8341 gctgactcaa cttcatgacc cgctcgttca gcatcgcgtt gtcccgttgc gtaagcgaaa
     8401 acattttctg caattccacg aaggcgctct cccattcgtt cagccgctgc atatagtcct
     8461 gttgcagttg ctctaatgcg ttcagcaaat gtttttccag ctccgtcatg ctctgtcact
     8521 cctgtaccag tctcactgca ttcagtttgc cgttgt
//
