LOCUS       NZ_CP015391            11090 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Klebsiella pneumoniae strain NY9 plasmid pNY9_6, complete sequence.
ACCESSION   NZ_CP015391
VERSION     NZ_CP015391.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04889856
            Assembly: GCF_001952895.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11090)
  AUTHORS   Huang,W., Wang,G., Sebra,R., Zhuge,J., Yin,C.,
            Aguero-Rosenfeld,M.E., Schuetz,A.N., Dimitrova,N. and Fallon,J.T.
  TITLE     Emergence and Evolution of Multidrug-Resistant Klebsiella pneumoniae
            with Both blaKPC and blaCTX-M Integrated in Chromosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11090)
  AUTHORS   Huang,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2016) Pathology, New York Medical College, 15 Dana
            Road, Valhalla, NY 10595, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. 3.5.0; HGAP Assembly v. 3
            Genome Coverage                   :: 300x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/04/2024 15:22:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,930
            CDSs (total)                      :: 5,807
            Genes (coding)                    :: 5,641
            CDSs (with protein)               :: 5,641
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 62 of 166
            Pseudo Genes (incomplete)         :: 110 of 166
            Pseudo Genes (internal stop)      :: 20 of 166
            Pseudo Genes (multiple problems)  :: 23 of 166
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP015391.1.
            Bacteria available from Guiqing Wang (hank.wang@wmchealth.org).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11090
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="NY9"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pNY9_6"
                     /geo_loc_name="USA: New York"
     gene            1..420
                     /locus_tag="A6P56_RS31590"
                     /old_locus_tag="A6P56_29785"
     CDS             1..420
                     /locus_tag="A6P56_RS31590"
                     /old_locus_tag="A6P56_29785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            726..989
                     /locus_tag="A6P56_RS31595"
                     /old_locus_tag="A6P56_29790"
     CDS             726..989
                     /locus_tag="A6P56_RS31595"
                     /old_locus_tag="A6P56_29790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            979..1278
                     /locus_tag="A6P56_RS31600"
                     /old_locus_tag="A6P56_29795"
     CDS             979..1278
                     /locus_tag="A6P56_RS31600"
                     /old_locus_tag="A6P56_29795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1340..1717)
                     /locus_tag="A6P56_RS31605"
                     /old_locus_tag="A6P56_29800"
     CDS             complement(1340..1717)
                     /locus_tag="A6P56_RS31605"
                     /old_locus_tag="A6P56_29800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            1786..1965
                     /locus_tag="A6P56_RS31610"
                     /old_locus_tag="A6P56_29805"
     CDS             1786..1965
                     /locus_tag="A6P56_RS31610"
                     /old_locus_tag="A6P56_29805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1993..2523)
                     /locus_tag="A6P56_RS31615"
                     /old_locus_tag="A6P56_29810"
     CDS             complement(1993..2523)
                     /locus_tag="A6P56_RS31615"
                     /old_locus_tag="A6P56_29810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2530..3261)
                     /locus_tag="A6P56_RS31620"
                     /old_locus_tag="A6P56_29815"
     CDS             complement(2530..3261)
                     /locus_tag="A6P56_RS31620"
                     /old_locus_tag="A6P56_29815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(3261..5225)
                     /locus_tag="A6P56_RS31625"
                     /old_locus_tag="A6P56_29820"
     CDS             complement(3261..5225)
                     /locus_tag="A6P56_RS31625"
                     /old_locus_tag="A6P56_29820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(6706..6855)
                     /locus_tag="A6P56_RS31635"
                     /old_locus_tag="A6P56_29825"
     CDS             complement(6706..6855)
                     /locus_tag="A6P56_RS31635"
                     /old_locus_tag="A6P56_29825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6922..7626)
                     /locus_tag="A6P56_RS31640"
                     /old_locus_tag="A6P56_29830"
     CDS             complement(6922..7626)
                     /locus_tag="A6P56_RS31640"
                     /old_locus_tag="A6P56_29830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(<7650..7847)
                     /locus_tag="A6P56_RS31645"
                     /old_locus_tag="A6P56_29835"
                     /pseudo=""
     CDS             complement(<7650..7847)
                     /locus_tag="A6P56_RS31645"
                     /old_locus_tag="A6P56_29835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            8009..11014
                     /locus_tag="A6P56_RS31650"
                     /old_locus_tag="A6P56_29840"
     CDS             8009..11014
                     /locus_tag="A6P56_RS31650"
                     /old_locus_tag="A6P56_29840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143775.1"
                     /translation="MPVDFLTTEQVESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHDEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISQSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     Typing          6025..6603
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2534..3261)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.33e-150"
ORIGIN
        1 atgagacgtt ccggcagcaa actgtgtctt atcgccatga taacagcgac agcaactctc
       61 actgcctgtg ctccaaaggg cagcgtggaa cagcataccc ggcattacgt atatgcttca
      121 gatgacggtt ttgatcccaa tttttccacc cagaaagctg acacaacacg aatgatggtg
      181 ccttttttcc ggcagttctg ggatatgggc gcaaaggata aagcgaccgg aaaatcacgg
      241 agtgatgtgc agcaacgcat tcagcagttt cacagtcagg aatttttaaa ctcactccaa
      301 ggcacaacac aatttgcggg taccgattac cgcagcaaag acctcacacc gaaaaaatca
      361 aggctactgg ctgacaccat ttcagctgtt tatctcgatg gctatgaagg cagacagtaa
      421 gtggatttac cataacgcgt cattacgcgc accgctcaga cgggctcagg gcgtttccgg
      481 cggctacggg ataaaacagg caacaacccc cccggaaaac tgccggaaac gcgcccgata
      541 aattttatcc taatgcatgc ctatgcacgc aactgcatgg catatgtttt gacgtaaaat
      601 ggatgccgta agcccttttt taccgacttt ctgcttcatc agcctgactg acacgaccat
      661 attctttcag accgccccct gctaaacttc cttcatattg ccgcattaac ccgggaggct
      721 ccgcaatgag caacactaca cactacgaaa acgccaactt tcttcgggaa ctggctgaaa
      781 gcctgccccg catactgccg gaaggtggcc cggacaaagc tgcgctgttg cagcgactgg
      841 ctaacgaaga actggcccag gcagagtatg aagatcaggt tcgcgccaaa gtgaccgccg
      901 cccgtgctga cacgcgtccg ggtatgacca cggaacaact ccgccagcgc ctgcatggcc
      961 gttatcagga actgcgcgat gccgtatgac gtggaatgga agcagggagc catcgaggat
     1021 gtaacgacgc tgtttgacta tatcgcggaa aattcatccc tgtgggatgc gcagaacgtt
     1081 accgaccggg ttctcgctgc ggcggacaaa ctggccgact tcccgcgact ctatgaagtg
     1141 gactcacgct atggcgagga cgttcgccgg atcagtctga tgggacagca tgtcctctat
     1201 gatgttgatg accagaccag aaaagtccgg gttctggccg tggtcggcca gcgccagaat
     1261 ccccatattg ttctctaata accggtgacg gggacgcaat tcattgcgtg gccgtcgccg
     1321 gttggacaag cacagcgcgt cagcgttttc cccacggcca ccaccggcgc ggtgttccgg
     1381 ccggggtctc tgctgtctcc ggtttgtgtt ccagcagtct catggactgc atttcctgcc
     1441 gtagcccggc cacctcctcc ctgagtgctt ttatctccgc gatcaacgcg tcggaggcca
     1501 ttttttcagt ctcacccgac agtctcaact cgtcccgctc gtgagtctca tcctgtctta
     1561 actcaccgta cacccggata agctcgctgg tatccaccac ccggccgcca tcagcttccg
     1621 tacggtatga tacgcggcct ttcgccatat cccgatacag ggaacttcgg gctttacccg
     1681 tcagttccgt cgcctgtctc accgtcaccc acgccattgt ctcaccccgc tatttttgag
     1741 acagtatacc tgacaggcgc acaccatgcc cggaaggaaa gaatcatgac aaaacaggaa
     1801 aaaacagccc tcaatatggc taaatttatt aaggagcagt cgctgctatt gctcgataaa
     1861 ctgaatgagc tggatatgga taccccggcc gacatgtgcg agcggctaca cgaggatgcg
     1921 gaaacactct ggcaggtgat gcgggataag ctcggggagg aataacagca ccggcatggc
     1981 cggtgctggc atttattttt tgccgaacgg ccaccaccgg cgggcaggtt tgtcctccgg
     2041 cagttcaggt accggcacgt taaaatagcg gctgttatat tcccgcatca gctccgcttt
     2101 cagcttctcg ttcaccgcgc tttccccgtg ccgctcactc accacgtcaa tcatcgcgtt
     2161 aatctgctcc ggtgagcact tcccggcatc aaccacctgc acaccgttct cgatggcgaa
     2221 ccgctccacc ttgcgataga ggtaatcaca cagcttcatt cctgtgggtt tgttccgttc
     2281 ccattgcgga tttttaccag tgaaaaagta cgtggctttt tcgtcgctgt tgagccgctc
     2341 gtcatacatc ccgatggatt tcatcatcgc cagatgctgg gcccggtcga caaaacagaa
     2401 aacatacgcc ccggtctgcc aggcgagcac caccatatct tccagatgct cgctggtcgg
     2461 catgatgcag cgctcgccgt tccggtcctc cgcgtcttca ataaatacca tgtacagcgc
     2521 cataatccct cactccgttg tcataaattt aaagcacgca aacaaccgtt cacgatccag
     2581 ctctgccgtt ttgcgggcct cgccggtctg tgggttcacg atttcatcca ccgacaggat
     2641 tgcccgctgc accctgacct gcgtcgcccg gtccgggcag atgtaccaga tttcatcgat
     2701 gccgttagct ttgcggttga acagataccc ggacaccact tcctgatacc ggcgacggct
     2761 cttgacggtg cgctccacct caaacgccac cgttttaccg tccagctccg ccagggcgtc
     2821 cggcactttc atgcctttaa gactcagacg gtgcagaatg cgccagccgg tggcccctct
     2881 ggcttccata tcaaggcgca gggattgcac ggccagccgg tgcggcagat gtgctgccga
     2941 cacccttgac ggctgaaacg gaataacatc cgccagcggt tcgtcctcgt cgaacgagaa
     3001 cgccacgcca tgcggcgtca gcccccacac gctgacctga cggccgtcca ccggatattt
     3061 ggtccggacc agcagccctt tcgccaccag gctgtcgagg aagcggtaga ccggctgcac
     3121 cgccgcaatc cccagccgcc gcgcaaccag ctccgccgtg gaacacagct cttcccgcaa
     3181 ccagcgcagg gtcagccggg ttttctccgc gttccgcgcc tgtcgtttcg cgtggctgac
     3241 gttgtacagc tccagcgcca tcagtaaatc cccgcaaaat catcatccgc tttcggcccg
     3301 ccgccgtcgg ccgggggccg gaactccgcc aggggatcgg ttttcgccag caccgggaaa
     3361 cgctgcaacg ggatttcccg gcgctcaacc cgcagcggac tgctgaacgc catccgggcc
     3421 acacccagcc cggtcatgac cgcgaacccg tccggcaggt gttgcagggt gttcacgtca
     3481 aacagcggcc gcgcctcctt ctggatgaag ctgtccccgc tgatatgctc cacgttcccc
     3541 gcctcccggc tggcacggcg gcgctccacg tccaccctga tttccccggt ctgccccgcc
     3601 gcccactgcg cggactccag gctggccgcc cggtaaaacc agcggatcgg cgtgttatcc
     3661 agtaccgtgg ttttcacaaa ttcagcgggc agatcctgcc cgcactgccc gaaatcgccc
     3721 agcgactggt gcgcgagcaa cagattgcag tcccggctgc gcagcgtccc cagtgcgttc
     3781 agtacatact tcgacagcag atatttaatt tcatcgagca tcacgctgac gtgtggccac
     3841 tcccggaact cgtcccgcgc aatgatgatt tgcgcgcagc gggcaaacag cattttttgc
     3901 acacggatca ccgcctcgtc gtccatcgag ccgataacgt acaggcagcc gccgccgttg
     3961 ataatccccg ccacatcgat gccactgtcc gtctgcagga cgctcaggtc agcgacgttt
     4021 tccagcctag tgatcaaccc cttcacgtcc tttttcaggg cctccggcag cgcgtacgcc
     4081 gccgccagca cctgctgaat gtttgccccc tgcggaaact gctccgcaat cagcttcgcc
     4141 gctttctgct cgctgatgcg gtagtggtcc gccacgtcgc cggtctcgcc caggttaagc
     4201 ccggcaatca gcaggttttt cagcgcgtac tggtcgagat cgcggaacag attcagctgc
     4261 gccggtttcc ccggccgcag atccagcagg gtgaagtccg gacagtgcgc ccggaagacg
     4321 tgcggcgccc atgcgtcccc tttggggtcg aagtacacca ccgcgtcccc gaaatcgcgt
     4381 acgcagcgaa tgagcgcgtt ggtggccatc acgctcttgc cgctcccggg caggccgagg
     4441 atctgaatgt tggtcttgcg ccaggtatca cgcggaacca gcaccggctt gccctgctca
     4501 tccttaccca gaaacaccgc ctgctcctgc cgggcctgct ccgaggaaaa atcggccagc
     4561 gcattaaccg gtaccggctc cccgaaatgc aggtggcgca cgtcggtcat ctctcctgtc
     4621 cgctggctgc tgcgccggct ctcctgattc agtttcgcgt tcaccgcctc gtcagcagcc
     4681 gcaaaatgac cgtcaagcag caggaacgcc agcagccgcg ccaacacaat cccgacagcc
     4741 agcccgcccg cgcaccaggc aatcccgaac aggttagccg gctgcagcag gtaataatct
     4801 gcgtttgcgt agaggtccgg acgcaggacg gtttctcccc agctctcgat aaacacggcg
     4861 acaaccagcc cccccagaag ggccagaccg gccgcgccgg tcagccagta cgcgtagttc
     4921 cagacatgtc gacgtgccct gaccatccgc ccgggcgaat ccagcgaggc cagcaccgaa
     4981 aatggcgctg gccgtttatt atcgcgctta tagcgatcgg catactcccg tcgctcctta
     5041 tgactcagcc ccacaaaacg catccgtccg gcccaaagtt cagccagacc gaaaaacagg
     5101 ccgataacta ggaaagcccc ggccagaccg taatactcct ggtacagcca gctgacccac
     5161 ggataaatct gccgggccag ctccggaatg tgccacttcc aggcaagcga atccgtatta
     5221 aacatgttct ctctccttca gacgatggtt tgccggcgtg atgtgcagcc cccgctgcac
     5281 aagccgcgga catgccccaa ggcatggcgg cggcggtcac gtccgtggtg attgcgttca
     5341 gctttcacca cggaggccag cctctttccg ccccatgcgg aaaaggcgca gccccggagg
     5401 aacaacgagc acgccccagc gtgtgagagg aaaaggcggc accccagtgc ggtcttttcc
     5461 ggtgttccgg cccccgtcag agttcgccat agcgagcggc tgaccacccg caaaatcagg
     5521 agcagctttg ctgcttcgcc aggtgcgtcc agcaccgtgg atttttgtgg gtggtcaggc
     5581 gtgagcctga cgggggccgg gtgaatttct ggcgtggccg gcgtccagcc ggacggagcc
     5641 agaaagaggg ggagagaccg tgcgtcggtg gcgtccagcc agcgtaagca ggggatatcc
     5701 ccctgaggtc agccacgccc tcgcggggat gacctgtatt tctccgtaag gagaaaaaag
     5761 caacagagat aggttaacgt aaaatgttca gcgaaaaacc gatatgattc acctatcctg
     5821 gtaatttatt atcagggaat ataaagactt aatcagggga caatatgcca catcagcgcg
     5881 tccggtgtcc gtcttgtagg tgccggggaa attgcgcctc ctcgctcact cggtcgctac
     5941 gctccggccg tgagactgcg gcgggcgctg cggacacatg caaagttacc cacagattcc
     6001 gtgaataagc aggggactaa catgtgaggc aaaacggcaa ggccacgcag gtggcgtttt
     6061 tccataggct ccgccctcct gactgagttc acataaacag acgctttttc ggtgcatctg
     6121 tgggagccgg tggctcaacc atgaatcagt caggagtggc gaaacccgac aggacttaaa
     6181 gatcatcacc gtttccgatg aatcgctccc tcttgcgctc tcctgttcca accctgccgt
     6241 ttcccggata cctgttccgc ctttctccct tgcgggaagt gtggcgcttt ctcatagctc
     6301 acactctggt atctcggctc ggtgtaggtc gttcgctcca agctgggctg taagcaagaa
     6361 ccccccgttc agcccgactg ctgcgcctta tccggtaact gttcacttga gtccaacccg
     6421 gaaaagcacg caaaacgcca ctggcagcag ccattggtaa ctgggagttc gcagaggatt
     6481 tgtttagcta aacacgcggt tgctcttgaa gtgtgcgcca aagtccggct acactggaag
     6541 gacagatttg gttgctgtgc tctgcgaaag ccagttacca cggttaagca gttccccaac
     6601 tgacttaacc ttcgatcaaa ccacctcccc aggtggtttt ttcgtttaca gggcaaaaga
     6661 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctact gaaccgcgat
     6721 ccccgtcagt tcagaggagg acgatggtgc caccgttcca ccctgaacat cccggatata
     6781 gtttgcctga catgcgacca gaagaaagcc tgaaaaaatc aatataaaaa gaaaaacaac
     6841 ctttaccttt ttcatatacc ttccctgtag gcactgttgc aaagttagcg atgaggcagc
     6901 cttttgtctt attcaaaggc cttacatttc aaaaactctg cttaccaggc gcatttcgcc
     6961 caggggatca ccataataaa atgctgaggc ctggcctttg cgtagtgcac gcatcacctc
     7021 aatacctttg atggtggcgt aagccgtctt catggattta aatcccagcg tggcgccgat
     7081 tatccgtttc agtttgccat gatcgcattc aatcacgttg ttccggtact taatctgtcg
     7141 gtgttcaacg tcagacgggc accggccttc gcgtttgagc agagcaagcg cgcgaccata
     7201 ggcgggcgct ttatccgtgt tgatgaatcg cgggatctgc cacttcttca cgttgttgag
     7261 gattttaccc agaaaccggt atgcagcttt gctgttacga cgggaggaga gataaaaatc
     7321 gacagtgcgg ccccggctgt cgacggcccg gtacagatac gcccagcggc cattgacctt
     7381 cacgtaggtt tcatccatgt gccacgggca aagatcggaa gggttacgcc agtaccagcg
     7441 cagccgtttt tccatttcag gcgcataacg ctgaacccag cggtaaatcg tggagtgatc
     7501 gacattcact ccgcgttcag ccagcatctc ctgcagctca cggtaactga tgccgtattt
     7561 gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc ctttgaatgg
     7621 gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcaccacc gactatttgc
     7681 aacagtgcca tcctcagcaa atccagccct tcccgatctg ttgaactgcc ggatgccttg
     7741 tcggtaaaga tgcggttagc ttttacccct gcatctttga gcgctctgat ctgaatatcg
     7801 agggactgct ggctggttga gacccgcgca taaccaaaaa ttcgcataaa aatgtacctt
     7861 aaatcgaata tcagacacga tgtgtctatt atgccaaaat gacgatttaa tggacactca
     7921 aacgaagccg ttttactatg tctgataatt tataatattt cgaacggttg cagttgtgtt
     7981 aaaaaagccg tcaggcaggg aggccgatat gcccgttgat tttttgacca ctgagcaggt
     8041 tgagagttat ggcaggttca ctggcgaacc cgatgaactt cagctggcgc gttattttca
     8101 tcttgatgaa gcggataaag aatttatcgg gaaaagccgg ggtgatcaca atcgacttgg
     8161 tattgccctg caaatcgggt gtgtgcgttt tctgggcact tttcttactg acatgaatca
     8221 tattccttcc ggcgtccggc attttaccgc cagacagctc gggattcgtg atatcaccgt
     8281 tcttgcagaa tacggtcaga gggaaaatac ccgccgtgag catgcagcgc tgatacgtca
     8341 gcactatcag tatcgtgaat ttgcctggcc ctggacattt cgccttaccc gtcttttata
     8401 tacccggagc tggataagca acgaacgtcc tggcctgctt ttcgacctgg cgacagggtg
     8461 gcttatgcaa catcgtatta ttctccccgg agccaccacg ctgacccggt tgatttcaga
     8521 ggtaagggaa aaggcgacgt tgcgcctgtg gaacaaactg gcactgatac cgtcagccga
     8581 acagcgttca cagctggaga tgctgctggg gccaactgat tgcagccgcc tgtctttact
     8641 ggaatcactg aaaaaaggcc ctgtgaccat cagtggtccg gcgtttaatg aagcaattga
     8701 acgctggaaa actctgaacg attttggcct gcatgctgaa aacctgagta cactcccggc
     8761 tgtgcgcctg aaaaatctcg cacgttatgc tggtatgact tcggtgttca atattgccag
     8821 gatgtcaccg cagaaaagga tggcggttct ggttgccttt gtccttgcat gggaaacgct
     8881 ggcgctggat gatgcactgg acgttctgga cgccatgctg gccgttatca tccgtgacgc
     8941 cagaaagatt gggcagaaaa aacggctccg ctcgctgaag gatctggata aatctgcatt
     9001 ggcgctcgcc agcgcatgtt cgtacttgct gaaagaagaa acaccggacg aatcgattcg
     9061 tgctgaggtg ttcagctaca tccctaggca aaagctggct gaaatcatca cgcttgtccg
     9121 tgaaattgcc cggccctcag acgataattt tcatgacgaa atggtggagc agtacgggcg
     9181 cgttcgtcgt ttcctgcccc atctgctgaa taccgttaaa ttttcatccg cacctgccgg
     9241 ggttaccact ctgaatgcct gtgactacct cagccgggag ttcagctcac ggcggcagtt
     9301 ttttgacgac gcaccaacgg aaatcatcag tcagtcatgg aaacggctgg tgattaacaa
     9361 ggaaaaacat atcacccgaa ggggatacac gctctgcttt ctcagtaaac tgcaggatag
     9421 tctgagacgg agggatgtct acgttaccgg cagtaaccgg tggggagatc ctcgtgcaag
     9481 attactacag ggtgctgact ggcaggcaaa tcggattaag gtttatcgtt ctttggggca
     9541 cccgacagac ccgcaggaag caataaaatc tctgggccat cagcttgata gtcgttacag
     9601 acaggttgct gcacgtcttg gcgaaaatga ggctgtcgaa ctcgatgttt ctggcccgaa
     9661 gccccggttg acaatttctc ccctcgccag tcttgatgag ccggacagtc tgaaacgact
     9721 gagcaaaatg atcagtgatc tgctccctcc ggtggattta acggagttgc tgctcgaaat
     9781 taacgcccat accggatttg ctgatgagtt tttccatgcc agtgaagcca gtgccagagt
     9841 tgatgatctg cccgtcagca tcagcgccgt gctgatggct gaagcctgca atatcggtct
     9901 ggaaccactg atcagatcaa atgttcctgc actgacccga caccggctga actggacaaa
     9961 agcgaactat ctgcgggctg aaactatcac cagcgctaat gccagactgg ttgattttca
    10021 ggcaacgctg ccactggcac agatatgggg tggaggagaa gtggcatctg cagatggaat
    10081 gcgctttgtt acgccagtca gaacaatcaa tgccggaccg aaccgcaaat actttggtaa
    10141 taacagaggg atcacctggt acaactttgt gtccgatcag tattccggct ttcatggcat
    10201 cgttataccg gggacgctga gggactctat ctttgtgctg gaaggccttc tggaacagga
    10261 gaccgggctg aatccaaccg aaattatgac cgatacggca ggtgccagcg atcttgtctt
    10321 tggccttttc tggctgctgg gataccagtt ttctccacgc ctggctgatg ccggtgcttc
    10381 ggttttctgg cgaatggacc atgatgccga ctatggcgtg ctgaatgata ttgccagagg
    10441 gcaatcagat ccccgaaaaa tagtccttca gtgggacgaa atgatccgga ccgcaggctc
    10501 cctgaagctg ggcaaagtac aggcctcagt gctggtccgt tcattgctga aaagtgaacg
    10561 tccctccgga ctgactcagg caatcattga agtggggcgc atcaacaaaa cgctgtatct
    10621 gcttaattat attgatgatg aagattaccg ccggcgcatt ctgacccagc ttaatcgggg
    10681 agaaagccgt catgcagttg ccagagccat ctgtcacggt caaaaaggtg agataagaaa
    10741 acgatatacc gacggtcagg aagatcagtt gggagctctg gggctggtca ctaacgccgt
    10801 cgtgttatgg aacactattt atatgcaggc agctctggat catctccggg cgcagggtga
    10861 aacactgaat gatgaagata tcgcacgcct ctccccgctt tgccacggac atatcaatat
    10921 gctcggccat tattccttca cgctggcaga actggtgacc aaaggtcatc tgagaccatt
    10981 aaaagaggcg tcagaggcag aaaacgttgc ttaacgtgag ttttcgttcc actgagcgtc
    11041 agacccctat aactcaatga gaaaaaagaa ttaaaggtaa ggagacgctc
//
