LOCUS       NZ_CP015245             8122 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Escherichia coli O91 str. RM7190 plasmid pRM7190-1, complete
            sequence.
ACCESSION   NZ_CP015245
VERSION     NZ_CP015245.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04623227
            Assembly: GCF_002716885.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O91 str. RM7190
  ORGANISM  Escherichia coli O91 str. RM7190
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Parker,C.T., Cooper,K., Huynh,S. and Sams,C.
  TITLE     Genome Sequence of Escherichia coli O91 str. RM7190
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8122)
  AUTHORS   Parker,C.T., Cooper,K., Huynh,S. and Sams,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2016) Produce Safety and Microbiology Research
            Unit, USDA ARS, 800 Buchanan St, Albany, CA 94710, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2016
            Assembly Method                   :: SMRT analysis v. Feb-2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 03:48:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,916
            CDSs (total)                      :: 4,791
            Genes (coding)                    :: 4,624
            CDSs (with protein)               :: 4,624
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 167
            CDSs (without protein)            :: 167
            Pseudo Genes (ambiguous residues) :: 0 of 167
            Pseudo Genes (frameshifted)       :: 84 of 167
            Pseudo Genes (incomplete)         :: 91 of 167
            Pseudo Genes (internal stop)      :: 37 of 167
            Pseudo Genes (multiple problems)  :: 41 of 167
            Pseudo Genes (short protein)      :: 2 of 167
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP015245.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8122
                     /organism="Escherichia coli O91 str. RM7190"
                     /mol_type="genomic DNA"
                     /strain="RM7190"
                     /serotype="O91"
                     /isolation_source="pig feces"
                     /db_xref="taxon:1055545"
                     /plasmid="pRM7190-1"
                     /geo_loc_name="USA: Monterey, California"
                     /lat_lon="36.58 N 121.66 W"
                     /collection_date="16-Sep-2008"
                     /collected_by="USDA ARS"
     gene            join(8094..8122,1..2191)
                     /gene="mobQ"
                     /locus_tag="I51_RS25410"
                     /old_locus_tag="I51_25085"
     CDS             join(8094..8122,1..2191)
                     /gene="mobQ"
                     /locus_tag="I51_RS25410"
                     /old_locus_tag="I51_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_024224537.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKEQDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSLGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            2424..4169
                     /gene="col"
                     /locus_tag="I51_RS25415"
                     /old_locus_tag="I51_25090"
     CDS             2424..4169
                     /gene="col"
                     /locus_tag="I51_RS25415"
                     /old_locus_tag="I51_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="WP_009451403.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAVPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMSGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKA
                     TGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRRKFWEEVSKDPELSKQ
                     FNPGNKKRLSQGLAPRARNKDTVGGRRSFELHHDKPISQDGGVYDMDNLRITTPKRHID
                     IHRGQ"
                     /name="col"
     gene            4172..4429
                     /locus_tag="I51_RS25420"
                     /old_locus_tag="I51_25095"
     CDS             4172..4429
                     /locus_tag="I51_RS25420"
                     /old_locus_tag="I51_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000421100.1"
                     /translation="MELKNSISDYTETEFKKIIEDIINCEGDEKKQDDNLEHFISVTEH
                     PSGSDLIYYPEGNNDGSPEAVIKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            4491..4634
                     /locus_tag="I51_RS25425"
                     /old_locus_tag="I51_25100"
     CDS             4491..4634
                     /locus_tag="I51_RS25425"
                     /old_locus_tag="I51_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            5100..5240
                     /locus_tag="I51_RS25435"
     CDS             5100..5240
                     /locus_tag="I51_RS25435"
                     /inference="COORDINATES: protein motif:HMM:NF020569.4"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /protein_id="WP_231237649.1"
                     /translation="MYEWDSQHGELEGYRASDGQHLGSFDPKTGNQLKGPDPKRNIKKY
                     L"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            5250..5507
                     /locus_tag="I51_RS25440"
                     /old_locus_tag="I51_25110"
     CDS             5250..5507
                     /locus_tag="I51_RS25440"
                     /old_locus_tag="I51_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_000523347.1"
                     /translation="MGLKLHINWFDKTTEEFKGGEYSKDFGDDGSVIERLGMPLKDNIN
                     NGWFDVIAEWVPLLQPYFNHQIDISGNEYFVSFDYRDGDW"
                     /name="colicin E3-like toxin immunity protein"
     gene            5527..5793
                     /locus_tag="I51_RS25445"
                     /old_locus_tag="I51_25115"
     CDS             5527..5793
                     /locus_tag="I51_RS25445"
                     /old_locus_tag="I51_25115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000861936.1"
                     /translation="MKVELKKSISDYTEAEFLELVKKICRAEGATEEDDNKLVREFERL
                     TEHPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            5855..5998
                     /locus_tag="I51_RS25450"
                     /old_locus_tag="I51_25120"
     CDS             5855..5998
                     /locus_tag="I51_RS25450"
                     /old_locus_tag="I51_25120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000724383.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            6495..7422
                     /locus_tag="I51_RS25460"
                     /old_locus_tag="I51_25125"
                     /pseudo=""
     CDS             6495..7422
                     /locus_tag="I51_RS25460"
                     /old_locus_tag="I51_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(7640..7915)
                     /locus_tag="I51_RS25465"
                     /old_locus_tag="I51_25130"
     CDS             complement(7640..7915)
                     /locus_tag="I51_RS25465"
                     /old_locus_tag="I51_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091751.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPKPPAKPQNH"
                     /name="hypothetical protein"
     BGC             1..8122
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2..330)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.394"
                     /coverage="92.0"
                     /evalue="8.58e-169"
     Typing          complement(7920..8093)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          6496..7422
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.336"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..2188
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.903"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 catttcgcga aaaaacggct acagtgctgt tgcttctgct gcctaccgtt ccggctctgt
       61 catacccgat gaccgtaccg gattaatcca cgattacacc cgtaaacgcg gcgttgatga
      121 tgcggtcatt ctcacccctg cgaatgcacc gtcctggtgt ggtgaccgtt ccgttctctg
      181 gaatgcggtc gagaaagccg aacagcgccg gaactcccag ctggcaaggg agattgaact
      241 cgccattccc cgtgagattt cccgcgaggc cgcacgggag accgttctcg cttttgtccg
      301 ggaaaacttt gtcagtcggg gcatgattgc cgatgtggcg ttccatcaca tggaccggac
      361 caatccccat gcgcacatca tgctgaccac gagagctgtc ggggaaacgg gattcgcagg
      421 aaaggtcagg gactggaacg accgggcact cgccgagacg tggcgcgcat catgggctga
      481 ccatgcgaac agagcgcttg cgaacgccgg ctaccaggaa gagatagacc atcgttcata
      541 cgagcgtcag ggactggaga aagcgccggg ccttcacctc ggaaaggctg cctgtgcgat
      601 ggaaaaacgg ggaatggaaa cagaacgcgg tgagcagaac cgcctgatta acagccttaa
      661 cctggaaata caggtttccc gcacaaggct ggcagtcatc caggtacagg agaacgtgcg
      721 gaaacgggaa ctcagcgatg ccgcacgtcg tgcagcggaa gcccttaacc tgaccattcc
      781 cgctgcgaat gcctcagcgg ataccctgcg ggaattcatt gccacgttgc cgcaggaatg
      841 cggcaacgcg tgggagatga ccccggagtt cctggcgatg agcgggaagg tgaacgacat
      901 cgaacgtgag gggaatgcgc tgctgaaaga gcaggccatt ctcgaaaagg agatgaccgg
      961 actcaaaaaa gcacgccctg tcgcgtccct tctgtcagag attcccctga tgacatgggc
     1021 tgaaccggaa taccgcaaaa gacaactccg tttctggaaa ctcgggaaac agattgaatc
     1081 tcttcgccgc acctacaggg ccgtgaaaga acaggacatt cccgcccgtc gtcaggcctt
     1141 tgaaacgcag tggaatacgt ggattgcgcc gggaatggca gagctgaaag aaaaactgtc
     1201 agcacgggaa gcggagcggc gcagggagga agccgaagcg gaagcgcgcc ggaaggaaca
     1261 ggagcatgag gcgcggctga aacgtcatga taaccaccgt ctgagccgtg aaacggcatt
     1321 agtcggggtt attacggagc tggggcgtgc cagagagccg ggaacgggca ggataacccg
     1381 ctacatgatg ctgagtaaca gagccggaga attcacggta tggggtgatg agctggcgca
     1441 ttacccccag agtgttcatg acccggtgaa tgtttacctg tcgctaggcg gggctgtgat
     1501 ggtctcggat atacgtgagg gaatgccaga atctcatgag acgatggcgc ggcctgagcg
     1561 tgtgagaatg tattccggtg cgacggtccg gcatgtactg gaacagatgc gccaggggtg
     1621 gccctcttac ggttttccgg cgctgccgca tcactggccg gataattttt atttcagcga
     1681 cgaccgcagg cccgtagcct ctccgctgcc gtcagcgcac cgggtggata tcaccgctga
     1741 tgctgtaccg gaacaactca tgcctgttgt attttcgaga gagggaaaca gcaggacgct
     1801 gaatctgttg ttatgcaaag ggccggagga aatgcttgtc gggtttgttc gccgggaagg
     1861 agggttttgc cccgttcttg tgcttccgtc gccggattac agtcatctga tagtcagcac
     1921 catcacggag aacggggtat gcctggcagg ttacggagaa gctataaacc atgatgagga
     1981 tactccgtac ccaccggaac cgcacctgat gcagttccgg ctcaaaggcc atcatgacag
     2041 gcttttggct gctgtccaca aaccggaaga gatgccggat tatctcttcc gtcaactcgg
     2101 ttttaatcag acctggcatg agtggaagcg ggacgaacag cacaggcaac aacaacgccg
     2161 ccccgggcac ttccggggca tgagtatgtg atgtccgggg ctgcaccccg gaccccgcca
     2221 acacatcacg ggccacaaaa ttttttgtgg cccgctctgc gttttctaag tgttatccct
     2281 cctgatttct aaaaaatttt ccacctgagc ttgacagaaa aaacgatgac gggtactttt
     2341 tgatctgtac ataaaaccag tggttttatg tacagtatta atcatgtaat taattgtttt
     2401 aacgcttaaa agagggaatt tttatgagcg gtggcgatgg acgcggccat aacacgggcg
     2461 cgcatagcac aagtggtaac attaatggtg gcccgaccgg gcttggtgta ggtggtggtg
     2521 cttctgatgg ctccggatgg agttcggaaa ataacccgtg gggtggtggt tccggtagcg
     2581 gcattcactg gggtggtggt tccggtcatg gtaatggcgg ggggaatggt aattccggtg
     2641 gtggctcggg aacaggcggt aatctgtcag cagtagctgt gccagtggca tttggttttc
     2701 cggcactttc cactccagga gctggcggtc tggcggtcag tatttcagcg ggagcattat
     2761 cggcagctat tgctgatatt atggctgccc tgaaaggacc gtttaaattt ggtctttggg
     2821 gggtggcttt atatggtgta ttgccatcac aaatagcgaa agatgacccc aatatgatgt
     2881 caaagattgt gacgtcatta cccgcagatg atattactga atcacctgtc agttcattac
     2941 ctctcgataa ggcaacagta aacgtaaatg ttcgtgttgt tgatgatgta aaagacgaac
     3001 gacagaatat ttcggttgtt tcaggtgttc cgatgagtgt tccggtggtt gatgcaaaac
     3061 ctaccgaacg tccgggtgtt tttacggcat caattccagg tgcacctgtt ctgaatattt
     3121 cagttaataa cagtacgcca gcagtacaga cattaagccc aggtgttaca aataatactg
     3181 ataaggatgt tcgcccggca ggatttactc agggtggtaa taccagggat gcagttattc
     3241 gattcccgaa ggacagcggt cataatgccg tatatgtttc agtgagtgat gttcttagcc
     3301 ctgaccaggt aaaacaacgt caggatgaag aaaatcgccg tcagcaggaa tgggatgcta
     3361 cgcatccggt tgaagcggct gagcgaaatt atgaacgcgc gcgtgcagag ctgaatcagg
     3421 caaatgaaga tgttgccaga aatcaggagc gacaggctaa agctgttcag gtttataatt
     3481 cgcgtaaaag cgaacttgat gcagcgaata agactcttgc tgatgcaata gctgaaataa
     3541 aacaatttga tcgatttgcc catgacccaa tgtctggcgg tcacagaatg tggcaaatgg
     3601 ccgggcttaa agcccagcgg gcgcagacgg atgtaaataa taagcaggct gcatttgatg
     3661 ctgctgcaaa agagaagtca gatgctgatg ctgcattaag tgccgcgcag gagcgccgca
     3721 aacagaagga aaataaagaa aaggacgcta aggataaatt agataaggag agtaaacgga
     3781 ataagccagg gaaggcgaca ggtaaaggta aaccagttgg tgataaatgg ctggatgatg
     3841 caggtaaaga ttcaggagcg ccaattccag atcgcattgc tgataagttg cgtgataaag
     3901 aatttaaaaa cttcgacgat tttcggagaa aattctggga agaagtgtcg aaagatcctg
     3961 agttaagtaa gcaatttaat ccaggtaata aaaaacgctt gagccaagga ttagctccac
     4021 gggctaggaa taaagatact gtcggtgggc gtcgctcatt tgagcttcat cacgacaagc
     4081 caatcagtca ggatggtggt gtttatgata tggacaacct ccgtatcact acgccgaaac
     4141 gccatattga tattcacaga ggtcagtaaa aatggaacta aaaaacagca ttagtgatta
     4201 cactgaaact gaattcaaaa aaattattga agacatcatc aattgtgaag gtgatgaaaa
     4261 aaaacaggat gataacctcg agcattttat aagtgttact gagcatccta gtggttctga
     4321 tctgatttat tacccagaag gtaataatga tggtagccct gaagctgtta ttaaagagat
     4381 taaagaatgg cgagctgcta acggtaagtc aggatttaaa cagggctgaa atatgaatgc
     4441 cagttgttta aggatgaatg actggcattc tttcacaaca aggagtcgtt atgaaaaaaa
     4501 taacagggat tattttattg cttcttgcag tcattattct ggctgcatgt caggcaaact
     4561 atatccggga tgttcagggc gggaccgtat ctccgtcatc aacagctgaa gtgaccggat
     4621 tagcaacgca gtaacccgaa atcctctttg acaaaaacaa agcgtgtcag gctgattctg
     4681 atgcgctttt tttgaaatgt cacaaaagtt ccatgtggga gatgggatct aaaatccctg
     4741 tgcagaacct tccatccggt gggagaaaac ttgtcatttt gacctgttcg ccattcgggg
     4801 gggggcgctt ggaaaacgga aattttccca cgctaagaga attgagttga acgagtggtt
     4861 acatctaggc cagctaactc actgaacgta aaggctgtac gaaatgttcc gaccgtccga
     4921 taaccatttg gcttacttcc gtacatttcc ttgcgtagat gtacagaatt gtacgatttc
     4981 ggacaccaaa accgctgttg tcccgccatt ctggcgattc ctgaaagtta tttgactaaa
     5041 aaatagtctt aacgtggttt attttccgaa ttccaagcgg accccataaa ggccgtaaga
     5101 tttatgaatg ggactcccag cacggtgaac ttgaagggta tcgtgccagt gatggtcagc
     5161 atcttggctc attcgatcct aagactggta atcagttgaa aggacctgat ccaaaacgaa
     5221 atatcaaaaa gtatctttga gaggatgtta tggggcttaa attacatatt aattggtttg
     5281 ataagacgac cgaggaattt aaaggtggtg agtattcaaa agatttcggt gatgatggct
     5341 cggtcattga acgtcttgga atgcctttaa aagataatat caataatggt tggtttgatg
     5401 ttatagctga atgggtacct ttgctacaac catactttaa tcatcaaatt gatatttccg
     5461 gtaatgagta ttttgtttca tttgattatc gtgatggtga ttggtgatca aatattctca
     5521 ggagagatga aggtggagct aaagaaaagt attagtgatt ataccgaggc tgaatttctg
     5581 gagcttgtaa aaaaaatatg tagagctgaa ggtgctactg aagaggatga caataaatta
     5641 gtgagagagt ttgagcgatt aactgagcac ccagatggtt cagatctgat ttattatcct
     5701 cgcgatgaca gggaagatag tcctgaaggg attgtcaagg aaattaaaga atggcgagct
     5761 gctaacggta agtcaggatt taaacagggc tgaaatatga atgccggttg tttatggatg
     5821 aatggctggc attctttcac aacaaggagt cgttatgaaa aaaataacag ggattatttt
     5881 attgcttctt gcagtcatta ttctgtctgc atgtcaggca aactatatcc gggatgttca
     5941 gggcgggacc gtatctccgt catcaacagc tgaagtgacc ggattagcaa cgcagtaacc
     6001 cgaaatcctc tttgacaaaa acaaagcgtg tcaggctgat tctgatgcgc tttttttttt
     6061 gaaatgtcac aaaaattcca tgtgggagat gggatctaaa atcctcgtgc agaactttcc
     6121 atccaggggg agaaaacttg tcgttttgag ccgttcggtg ttcagaacgc acgaaaccga
     6181 tcgcgcatcg ctttcgtgaa tagttatgca ggcccctgaa aacgattctg acgcgttttt
     6241 tcggttttgc ctggtgtttt cctgtctttt tgcgtttttt gcgtcagaac gcgtctgagg
     6301 gcgttttaag gggtgcgtac aacgggagtt atggtaaatg gatcgggttt tcgggaaggt
     6361 tcgacaggat ttgccgttgg gtgtagtgta agcgactgaa aaacaaacgc ccctgaaatt
     6421 cgtgtctctc cgccaagatt gtcacgagaa tacaggggcg aggtcggtcg ataacctgga
     6481 aggaatcgta acacatgagc gccgcgcttc aatacttcga agaaaattta ccccaccgtc
     6541 cctatcacac ggatgatctc gcttttggtc ttcgcatctc cggcaaaggg cgtgcgcttc
     6601 ttggcgcggt acatccagca gaaccagcct catgcgcagt tctggctggt ttttgacgtt
     6661 gaccgcgagg gggctgcgat tgactggagc gaccggaacg cccccgcgcc gaacatcacc
     6721 gtaaaaaatc ccgtgaacgg tcatgctcac ctgctctatg cactcaacat cgccgtgaga
     6781 accgcgcctg atgcgtcggt taaggcactg aagtacgctg ccgcgataga gcgtgcgctg
     6841 tgtgagaaac ttggtgcaga tgtgaactac agcggactga tttgcaaaaa tccgttccac
     6901 ctggaatggc tggtgatgga gtggcgcgag gaagcctata ccctcgatga actggctgat
     6961 tatctcgatt tgagcgcctc agagcgtcgt agcatcgata aacattacgg gatggggcga
     7021 aactgccacc tgttcgaaat gacgcgcaaa tgggcttaca gggcgattcg tcagggctgg
     7081 cctgtattct cacaatggct tgatgccgtg atccagcgtg tcgaaatgta caacgtatcg
     7141 cttcccgttc cgctttctcc ggcggaatgt cgggctattg gcaagagcat tgcgaaatac
     7201 acgcacagga acttcacgcc ggaaactttc gcacagtatg tggctgatac gcacacgcca
     7261 gaaattcagg ctgcacgtgg tcgcaagggc gggaaaattg gaggtgctaa gtctaagcgt
     7321 ggtgctgttg ccacatctgc gcgaacgttg aaaccgtggg agactcttgg aattagccgc
     7381 gcctggtatt accaactgaa aaaacgaggt cttgtagagt agaccaaata aaacctatat
     7441 cagataacag cgcctttttg gcgctttttt gagcagcttg gtttgttgct atttccctcg
     7501 ttgaatcccg caatggcgcg gctttccgca tgattgaggt tgtagcgctc gccgcagtct
     7561 catgaccgag cgtagcgagc gaatgagcga ggaagcgcaa aggcgtccgg tggtgcatgt
     7621 ggctcttacg cgccggggct tagtggttct gcggtttcgc aggtggtttg ggtagcttct
     7681 ccagctcgtt aatcagcggt tgtagtcggt tcacatccgc ctgtcttgtg acttcctttc
     7741 gcagaaactg gagcaggaac gcacgcagtt gcgcctcttc cggcctccgt accctcgcca
     7801 gcatggcagc ccccacaatg actttttgcg ccgtgtccag gctccggctc ttcgccttca
     7861 ggcgctgtaa tctggcctca gcttcggcaa tcttctgttc gagtgttctg ctcattttgt
     7921 gactccgtgc gcggtgaaaa atcgcatttt atcgcgtcac tggtagttgc aaaactgaac
     7981 tggcataatg cacggcacat cacgaagtgc gcacttatac aatctccact tcgtttcgat
     8041 tgtgtgcggt ctgcgacgct aaaagaaaac ggcaaaaagg cattacggca gaaatggcga
     8101 tttatcatct cagcatgaaa at
//
