LOCUS       NZ_CP015178             4227 bp    DNA     circular CON 14-MAY-2024
DEFINITION  Bacillus thuringiensis serovar alesti strain BGSC 4C1 plasmid
            pBMB4227, complete sequence.
ACCESSION   NZ_CP015178
VERSION     NZ_CP015178.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04628222
            Assembly: GCF_001640965.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis serovar alesti
  ORGANISM  Bacillus thuringiensis serovar alesti
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Wang,Y. and Sun,M.
  TITLE     Complete genome sequencing of Bacillus thuringiensis serovar alesti
            strain BGSC 4C1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Wang,Y. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2016) State Key Laboratory of Agricultural
            Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 1.9.0
            Genome Coverage                   :: 246
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001640965.1-RS_2024_05_14
            Annotation Date                   :: 05/14/2024 01:49:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,053
            CDSs (total)                      :: 5,914
            Genes (coding)                    :: 5,704
            CDSs (with protein)               :: 5,704
            Genes (RNA)                       :: 139
            rRNAs                             :: 12, 12, 12 (5S, 16S, 23S)
            complete rRNAs                    :: 12, 12, 12 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 94 of 210
            Pseudo Genes (incomplete)         :: 117 of 210
            Pseudo Genes (internal stop)      :: 65 of 210
            Pseudo Genes (multiple problems)  :: 50 of 210
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP015178.1.
            Source bacteria available from Prof. Ming Sun, State Key Laboratory
            of Agricultural Microbiology, Huazhong Agricultural University,
            Wuhan, PR China.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Bacillus thuringiensis serovar alesti"
                     /mol_type="genomic DNA"
                     /strain="BGSC 4C1"
                     /serovar="alesti"
                     /isolation_source="soil"
                     /db_xref="taxon:1440"
                     /plasmid="pBMB4227"
                     /geo_loc_name="Czechoslovakia"
                     /collection_date="1987"
     gene            306..848
                     /locus_tag="Bt4C1_RS28645"
                     /old_locus_tag="Bt4C1_29450"
     CDS             306..848
                     /locus_tag="Bt4C1_RS28645"
                     /old_locus_tag="Bt4C1_29450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360274.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000360273.1"
                     /translation="MDKNSEKKITVELNVDEFKKLQKLLELDEKNEQEQQKKENEKKNL
                     NFIQLYRENMPELRWLMANHNFASSLLFFIIEHMDNKNALACSYSIFEDYFGKSKMTIY
                     RGIKVLEENGFLSVLKMGTSNVYLVNEDLAWTNSNDKKKYAKYDGKILISKKENKDYVY
                     RKQFDRFKSLREREGLK"
                     /name="replication/maintenance protein RepL"
     gene            1001..1315
                     /locus_tag="Bt4C1_RS28650"
                     /old_locus_tag="Bt4C1_29455"
     CDS             1001..1315
                     /locus_tag="Bt4C1_RS28650"
                     /old_locus_tag="Bt4C1_29455"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001049715.1"
                     /translation="MNLKKYINNKFMVYCFEIGFESAIARVQQELQEVNLNHFIYQDLF
                     LCDPESIAKKTEEVTNTYHALLNRNTEQWFFIYRAKTLDFHSAKKLFKQLEELRLQSQH
                     "
                     /name="hypothetical protein"
     gene            complement(1359..1493)
                     /locus_tag="Bt4C1_RS32530"
     CDS             complement(1359..1493)
                     /locus_tag="Bt4C1_RS32530"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_256936609.1"
                     /translation="MISDTDNIMLFDGEPYKVDFINGQYIKTKIAWEQIPKHRHTELV"
                     /name="hypothetical protein"
     gene            complement(1516..2484)
                     /locus_tag="Bt4C1_RS28660"
                     /old_locus_tag="Bt4C1_29460"
     CDS             complement(1516..2484)
                     /locus_tag="Bt4C1_RS28660"
                     /old_locus_tag="Bt4C1_29460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_063676701.1"
                     /translation="MATTKLGNSKSCSKVINYAEKRAVVQSGVNCMPEFAKSQMKAVRM
                     VHGKDTGVQAHTIIQSFKPGEVTTEQANDLGRQLAESIAQGHQVAIYTHNDTEHIHNHI
                     VINSVHMETGRKYQSNASQIKLVKSENDRICKENGLSVASQKAEVRYTLAERSLLEKNE
                     RSWKDEVREVVDLAKQQTSNFQEFSEHLEEHGVGVLVRGKTITYTHMNENKKVRGKKLG
                     ELYTKEGVEDEFERRNTEQKRDTSNRDAETRFTSEVERTREKINGAKHDSSRVSEPNPR
                     LNEKPTIMDGIGLAAGRDVREEQKRRKRYEEWRRRSQERER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2466..2858)
                     /locus_tag="Bt4C1_RS28665"
                     /old_locus_tag="Bt4C1_29465"
     CDS             complement(2466..2858)
                     /locus_tag="Bt4C1_RS28665"
                     /old_locus_tag="Bt4C1_29465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001288267.1"
                     /translation="MSEERNAVSSNSLAKKKEPRRRDSIQISFRVNEQDYEKLKASADN
                     LSMSVTAFAKMKVQNARMMKPKFDKESSLQIITELNRIGNNVNQIAKYCNMRQSGRFGG
                     NQMDGLIEKNLKEVERELASLWQQLN"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          307..848
                     /name="rep_cluster_1931"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtaacgtttt gttttttact ctttacatct attacaacga ttcgttataa cacatcacgt
       61 ggagaagaac tctatttaga gatatagaaa gtacaagtct aaaaacgttg atatataagg
      121 gtttcagagt ttaataggta acatagcttg tgatacctat aggtaacata gcttgtgata
      181 cctatttgta gaacaatata acatttttgt ctttacatat atcataaaat gttataggat
      241 ataacaaaga ttatacatat aggtatcata gtgtgttacc ttcttgtaaa aaaaggggtg
      301 aaaaaatgga caaaaactct gaaaaaaaaa tcactgtaga attaaacgta gatgaattta
      361 aaaaacttca aaaattatta gaactagatg aaaaaaacga acaagagcaa caaaaaaaag
      421 agaatgaaaa aaagaatctt aatttcattc aactttatcg agaaaacatg ccagaactaa
      481 gatggttaat ggcaaaccac aatttcgcta gttcactttt atttttcata atcgaacaca
      541 tggataataa aaacgcatta gcatgctcat attcaatttt tgaagattac tttggaaaat
      601 caaaaatgac tatttacaga ggaatcaaag tacttgaaga aaacggattt ttgagtgtac
      661 taaaaatggg tacatcaaat gtttatttag taaatgagga tttagcgtgg actaattcaa
      721 acgacaagaa aaaatacgca aaatatgatg gaaaaatcct tatcagcaaa aaagaaaata
      781 aagactatgt atatcgtaaa caatttgacc gttttaaatc tttacgtgaa agagaaggtt
      841 taaaatgatt aaagattcaa acaaaaacaa ttaaaaaatg ttccctgcat actgaaatac
      901 agagaacact tatgcagata agattgagac ccctatttgc atactccagt atgcaggaac
      961 gttttctata aatcaaacta aattactgga ggctttactc atgaatttaa aaaagtatat
     1021 taacaacaaa tttatggttt actgctttga aattggattt gaatctgcta ttgctagggt
     1081 tcaacaggaa ttacaagagg taaatcttaa ccactttatc taccaagatt tatttttatg
     1141 tgacccagaa agtattgcaa agaaaacgga agaagtaaca aatacatatc atgccttact
     1201 caatagaaat actgaacaat ggtttttcat atatagagct aaaacattag attttcatag
     1261 tgcaaaaaaa ctctttaaac aacttgaaga attaaggtta caatcacaac attaacatac
     1321 aaaaggtgct gatccactac atggatagca cctttttgtt ataccaattc agtatgtcta
     1381 tgtttcggaa tttgctccca tgctattttt gtttttatat actgtccgtt tataaaatcc
     1441 actttgtaag gttcaccatc aaataacatg atgttatctg tatccgaaat cataggtaat
     1501 actgattttt tgggttcaac gctctctttc ttgagaacgg cgtcgccatt cttcataacg
     1561 ttttctacgt ttttgctctt ctctaacatc tctacccgct gcaagaccga ttccatccat
     1621 gattgttggt ttttcattaa gtctagggtt tggctcacta actcgactac tatcgtgttt
     1681 tgctccgttg attttctctc tagttcgctc aacttcgcta gtaaatcgtg tttcagcatc
     1741 tctatttgac gtatctcttt tttgttctgt atttcgtctt tcaaactcat cctctacacc
     1801 ttctttcgtg tacaactccc ctaatttctt tccacgtact tttttatttt cgttcatatg
     1861 cgtatatgta atggtttttc ctcgaaccaa cacacctact ccatgttctt ctaagtgctc
     1921 ggaaaactcc tgaaaattac ttgtttgttg ctttgccaaa tcaactactt ctcttacttc
     1981 atctttccag cttcgctcat ttttctctaa taatgaacgt tctgccaatg tataacgaac
     2041 ctctgctttt tggctagcca ctgaaagccc attttcttta caaattctat cattttcgga
     2101 ttttactaat tttatttggc tagcattact ttgatatttc ctacccgttt ccatatgtac
     2161 agaattaatc acaatgtgat tatgaatgtg ttccgtatca ttatgcgtgt aaatagcaac
     2221 ttgatgtcct tgtgcaatcg attcagcaag ttgtcgccct agatcgttag cttgttccgt
     2281 tgtcacttcc cctggtttaa aggattgaat aatagtatgt gcctgcacac ctgtatcctt
     2341 tccatgtacc atcctaacgg ctttcatttg cgactttgca aactcaggca tacaattgac
     2401 accactttgt acaaccgctc gtttctcggc ataattaatc accttagaac aactcttcga
     2461 attccctaat ttagttgttg ccataaggat gccaactctc tttctacttc tttcaaattc
     2521 ttttcaatca aaccgtccat ctgatttcca ccaaatcgac cggactgtct catattgcaa
     2581 tactttgcga tttggttcac gttgtttcca attcgattca attccgtaat aatctgcaaa
     2641 ctgctttctt tatcaaattt tggtttcatc atacgagcat tttgcacctt catttttgcg
     2701 aaagccgtca cactcatact taaattgtca gctgacgcct tcaatttttc gtaatcctgc
     2761 tcattcactc gaaagctaat ttgtatacta tctcttcttc ttggctcttt ctttttcgcc
     2821 aaagaattgc tagatactgc attacgctcc tcgctcattt tcaacccctc cagtcccgta
     2881 tttgagggtt gatattttgc ccttggctct ctatcaaccg ctatgacttc gatatgttta
     2941 tatctctagc atagcgcaac ctcaaacact tggcaagcga cgtatttgct agccacttta
     3001 cttcgttcag tgtctgcaaa taccacttgt tgggggacgt ttcactttcc cccaatcccc
     3061 cttttaaaaa caaaaaacga ggtctagctg ttcctcaatg aatgctattt attccacgtt
     3121 ttcttgacca cgagcctcta aaccctcaaa ctcccgaagg caatcccacc ggtcaaaaac
     3181 cgcccgatgg gaccacctcc cgtagtttgc atgagggttc ccctcatagt aaagaaactt
     3241 tcgcggcata atcgctctgt ttctctatac aaaaaagagt ctggatccag actcttttcc
     3301 ttgcctacta cggtaaggct ataccaattt aattcgcgct cactaaggta gagctactcc
     3361 aagtttcggt tttttattta gccgagcgta ctccgaaaaa ctgctcctat cttattaatc
     3421 attctaacac aaatcctgaa aaaataacga agtatagtat acctcaaagt atattatact
     3481 tctctctcat cttcgcactt gcgccgcaag ggaatggcaa ctgctttgct catttctttt
     3541 ctgtcatttt gtaccaagcc tttacaagat tccctatatt ctttctcctg tttagggaat
     3601 cttggcgaag acaacagact tctcacccct cagaaagccc tctgagcgtt tttaacttgt
     3661 ccgcatacaa aaatacttgg tgatcaaaac aaacgtctta aaaagattgt ataagccttt
     3721 aaaaaaggtt atcccccttg tttttgggtg tgaggaagtg gctggggtga agcgaagcga
     3781 ggggctagcc ccttttagtg tcatcaaaac atgaagtaag agtttattcg tgaacagtag
     3841 aattaggcgg ttcggcaggt acaattgcta atccaagtcg tgctacctaa ttcctcgttt
     3901 ttggtatgtt cggcttggcg atatgcccac atctgccgaa ttcctctaag caatggaaaa
     3961 aagggtggag atttttcaag tgatgttctt gaaaaatact acccgaccat tgcttagagg
     4021 aaagcttatt ttttatatag tcgaatccaa aacccaaaac catggtacaa gataagagag
     4081 gagagagttg ttgtaaaaat agtgtttaag atagatatat caagggttta agaggttttt
     4141 aggcattaca tgagtcatgt aagactatta cacgactcat gtaatgatgt tatatgagtc
     4201 gtgtaattat aattacaatg atttatt
//
