LOCUS       NZ_CP015144             4204 bp    DNA     linear   CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_732 plasmid pEC732_6.
ACCESSION   NZ_CP015144
VERSION     NZ_CP015144.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04621897
            Assembly: GCF_001617565.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Stoesser,N., Sheppard,A., Peirano,G., Sebra,R., Lynch,T., Anson,L.,
            Kasarskis,A., Motyl,M., Kazmierczak,K., Crook,D. and Pitout,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2016) Department of MIcrobiology (Research), John
            Radcliffe Hospital, University of Oxford, Headley Way, Oxford OX3
            9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.2.0
            Genome Coverage                   :: 1x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001617565.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 01:47:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,381
            CDSs (total)                      :: 5,268
            Genes (coding)                    :: 5,043
            CDSs (with protein)               :: 5,043
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 84 of 225
            Pseudo Genes (incomplete)         :: 167 of 225
            Pseudo Genes (internal stop)      :: 38 of 225
            Pseudo Genes (multiple problems)  :: 54 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP015144.1.
            Bacteria available from Johann Pitout, University of Calgary
            Laboratory Services, Calgary, Alberta, Canada, or Nicole Stoesser,
            Department of Microbiology, John Radcliffe Hospital, Headley Way,
            Oxford, UK.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_732"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC732_6"
                     /geo_loc_name="Thailand: Bangkok"
                     /lat_lon="13.76 N 100.50 E"
                     /collection_date="2012"
                     /collected_by="Merck Study for Monitoring of Antimicrobial
                     Resistance Trends (SMART)"
     gene            <1..358
                     /locus_tag="A4X18_RS31600"
                     /old_locus_tag="A4X18_27360"
     CDS             <1..358
                     /locus_tag="A4X18_RS31600"
                     /old_locus_tag="A4X18_27360"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_205248782.1"
                     /translation="TRKHQAKPEKRVRIVFRALHNYARKRCAIGFVRSEHRTAQNDKFS
                     PLVRGALTGFPVSKLHISPGQKKTDLGSVARIRVYGKYFSYRMLNIHKRKKCLSDALKQ
                     PFQKESALYDARKR"
                     /name="hypothetical protein"
     gene            1507..1716
                     /locus_tag="A4X18_RS27380"
                     /old_locus_tag="A4X18_27365"
                     /old_locus_tag="A5H92_RS27365"
     CDS             1507..1716
                     /locus_tag="A4X18_RS27380"
                     /old_locus_tag="A4X18_27365"
                     /old_locus_tag="A5H92_RS27365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(1782..2315)
                     /locus_tag="A4X18_RS27385"
                     /old_locus_tag="A4X18_27370"
                     /old_locus_tag="A5H92_RS27370"
     CDS             complement(1782..2315)
                     /locus_tag="A4X18_RS27385"
                     /old_locus_tag="A4X18_27370"
                     /old_locus_tag="A5H92_RS27370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(2318..3244)
                     /locus_tag="A4X18_RS27390"
                     /old_locus_tag="A4X18_27375"
                     /old_locus_tag="A5H92_RS27375"
     CDS             complement(2318..3244)
                     /locus_tag="A4X18_RS27390"
                     /old_locus_tag="A4X18_27375"
                     /old_locus_tag="A5H92_RS27375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            3421..3819
                     /locus_tag="A4X18_RS27395"
                     /old_locus_tag="A4X18_27380"
                     /old_locus_tag="A5H92_RS27380"
     CDS             3421..3819
                     /locus_tag="A4X18_RS27395"
                     /old_locus_tag="A4X18_27380"
                     /old_locus_tag="A5H92_RS27380"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(4023..>4204)
                     /locus_tag="A4X18_RS31605"
                     /pseudo=""
     CDS             complement(4023..>4204)
                     /locus_tag="A4X18_RS31605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          complement(2322..3244)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gacaagaaaa caccaggcca aaccagaaaa acgcgtcaga atcgttttca gggcgttgca
       61 taactatgca cgaaagcgat gtgcgatcgg tttcgtgcgt tctgaacacc gaacggctca
      121 aaacgacaag ttttctcccc tggtgcgtgg cgctctgacg gggtttcctg tttccaaact
      181 tcacatctcg ccaggacaaa aaaaaaccga cctggggtcg gtggcaagaa tcagagtcta
      241 tgggaaatat tttagttatc ggatgctcaa tattcacaaa cgcaaaaaat gcctcagtga
      301 tgccctaaaa caacctttcc agaaggagtc cgcgctttac gatgcacgga agagataaaa
      361 aaccccacaa aagtggggaa ccaagagaaa caacaattac aggttgttgg ttgtgttcga
      421 acaacgatga agtagctacg ctaaataata attaccgtac aggccctaca tattcaacac
      481 taaatttaag atcctgacga aggatttata aatgcacgaa aacacataga cagacaaatt
      541 cactaaattc atacacaaaa acaagtgtag ttttctaagt aaattagatt cgacaaccaa
      601 ttagtttatc cacatcgcat tccactcttg ctacaccaat cacatttctt gatatgtaat
      661 agcagaaaaa ataaaaccag acaccaatag accaaaaaac aaacattcaa caagaaacat
      721 tttgaacaat atcacaaaac tgtcacaacc atcatcagac tattgaaaaa aactggttgc
      781 tgttttataa aagcgactgt aacaacctcc actctatagt tccattagtt taaagtgtca
      841 cacaaactga gcatccattt gttttcctta cttttgcccc tgtattccaa ggggtttttt
      901 tataacttaa agattagcac accacacaaa aacaataaaa aaaaacgaca agttatacaa
      961 taaaaccagt actatccata cagctaatta catataaata ctattaaacc atctggacat
     1021 taaaaataaa ctcttataac ataaaattaa tgcggttccg gtaccatatg tcagggataa
     1081 tgtgcaaatg gaggtatcgg aactgcattt cagttcatta ttaattaatg acaaaaaaaa
     1141 cctctgcgag cgcagagggg gtaaggaatg ttcccggtca taatatgtcg atagtgttta
     1201 tatacataca gaacaagaac aacaaccagc caactaataa atattttatt ttttagagct
     1261 gaaaccttaa agctcacttc cattccatac attgttataa accttatgtt gaaaactgaa
     1321 agtaagtacg gcagacttat taagttgcgt gcagtctttt cacgttatac aaacaagaac
     1381 acctgcgaag ttatttctcc ttacatatcc ccctatgcca acgggggatt ttttctggtt
     1441 cactaacgca taaataatag tgacacccta tcattcacaa accgtgataa tataaggatt
     1501 atcatcatgg caaaattagg tgaaaacacc ccacatatta tcgatgctac tgtagatttt
     1561 atggcatcaa gccaggcatt tcgtgagtat ctgaaaaaac ttccccctga taacacaata
     1621 ccgccagctc ttactgatga gcacgtaccg ttatatatac gaaggctgga atactatcgt
     1681 ctgctttatc gaccaaagca gacagaatca tattgaaaaa aacggtgaac ggttcgcgaa
     1741 attctttcgc gggtcgtgag gcgttggaca agcgaagcgc gtcagttttt ccggctcttc
     1801 cccgtgacag gtttctccac cagtttatct tccagtgtca caactttctg atacaccgcc
     1861 gctgtggcgt tgtagttcct gtcgagtatt gttttcattc cccatccgag cacacagaac
     1921 ataaccagaa aagccactga caccccaccc agccatttcc agctataccc tttaagggtg
     1981 ttgtattcct gcacgatatt gctgtgaatt gtcgcaatat ccctgttcag ggcatccgcg
     2041 tttgccttca tgtttgcgac tgactcttcc agccgctgaa aatcctggct gatgcgctca
     2101 cctatcagtc ctgccacgtc cagcttctgc acctcatttc tgacttcatc cctgacctgt
     2161 tttgccacac cgtcagaaat atcccgatat ccgcgaagag tttcccttgc cacagccaga
     2221 gaatcagcaa cagctttctg ttgctccatt gcggtatgtt gcagatgctc caccgataac
     2281 aggagcgtaa gtaatttttc ttctgtgctg gccatgttca gaatcccata gaaaatccac
     2341 gtgacggtga tttaacccgt tcaggtgcag ttcggttctg tctgctcact ctgtctctca
     2401 tgtcaaagcc atgctcccgc gcaacatcat gttgcagcct tctgaccagt tcatgttgct
     2461 taaccggagt gtccagccat tccctgatgt catgcccgac cgttcgcgcc tgttcaagat
     2521 ggcgggaata gtccggttca gaccgttttt gcatgatgtc atgacgctgg gcatcatcaa
     2581 gccgtccggc agcttcaaaa cccagatgca cttcgggacg tcgttcaatg ccctgttctt
     2641 tcagcgtccg gcagtcaatg aagccccttt caaagccatg ctttctcata tgttcgttgt
     2701 gcttcacctc ccagcgtttt cgcaattccc gcaactgctc tttttgctga gtgagagtca
     2761 ggcttccgga atcctttttc gcacctcccc gctcgggata cctggcgtta tagcgccgga
     2821 aatactgttc cggtgtacgt tcaatgccat cattcacacg ctgactcatc atgatatgcg
     2881 catgaggctg ttcgccgccg tcaatgctcg ctttcggatt gtgaatggca aaactccagg
     2941 cgtgccgttc gccagcttcc tgacgtacaa aatcctgcac cagttcaagg cgctgttccg
     3001 gtgtcagttc acggggtaat gcaatttcaa gttccctata ggtcgaaccg ttcgcacgtt
     3061 caaactggtc agccgcctgc cagaaatggg aaggattatc cctggcccat tccggcatgt
     3121 ttccgtgtgc ggaatattcc aaatcctgac ggtcgcggta tttctctttg cgctcgatgt
     3181 aatcggcgtg gtttgcagcc tgccccttgc caccaaattt aacgctgagg tgataggttg
     3241 ccacggtttc agccctctgc aggagcgcac tgtttctcga cgaaggagag aaacatataa
     3301 gtgcgcttcc ttctaatttt acgaattagc acggaagata ccccctgcac ccgccgaccg
     3361 caagcgatat actgcacgac cacaacacac cgcaaaaaaa caccagggag catcacgaac
     3421 atgcgcatca gcaacatcga atggctgaag aaacggatcg gattcatcag gaagctcgga
     3481 gaacagaccg cacgacagag acagattatt gacctgatcg acaacgaagc cggactcaca
     3541 gaacaggaac gcaaacttct ccacgtactg gcaacagcgg aaaaaaacga cttacaggca
     3601 caggagagcg aacgaaaaca ggcggtccaa aaacgaatcg agggaaaaaa acaacgacgg
     3661 gaacgcaatc accggctctt tctcgcggct gggctactga tcgaagcagg actggtagac
     3721 acaaaaacag gcgagctatg ctataaaaaa gacaggatat tacaagcact taaggaactt
     3781 aagtacgatc tggagacatc cccaaaccca gacgcgtaag tgcctccatc cgtgttccga
     3841 ttccctggtg cttcctcgct cattcgctcg ctacgctcgg tcatgagact gcggcgagca
     3901 ctaccaccac aatcacgcgg aaagccgcgc cattgcaggg ctgaacgggg aaaaacggca
     3961 aaaaacctgc tgctcaaaaa gacgccagaa aggcgctgtt atctgatata ggcttatttg
     4021 gtctactcta cgagacctcg ttttttcagt tggtaatacc aggcgcgact gatccctaat
     4081 gcctcccacg gtttcagcgt gcgtgcagat gtagctactg tgctgcgctt agacttagaa
     4141 ccgcccttgc gaccgcgtgc agcctgaatt tctggcgtgt gcgtatcagc cacatactgt
     4201 gcga
//
